MD04G1022600.v1.1

U11 U12 small nuclear ribonucleoprotein 35 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009123
Physical Location & Seq
Reverse (-)
2644667 .. 2647181
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1022600.v1.1.491

Sequence Viewer

Length: 939 bp
ATGAGTGGGGGCGGACGAAACGTGAACGGAAACTTAAACGCGGTGTTCTACGCGGAGGCGTATCATCCGATTCAGGCGGGAAGCATAGACGGCACCGACACTCTCCCTCACGACAACGCCGTCTACCGAGCCTTCCTCTGCTCCTCCGCTGGCCTCTATGACCCCCAGGGGGACCCCAAACTCCTCGGCGACCCTTATTGTACTCTCTTCGTTGGTCACCTTTCTCATCTCACTACCGAACGCACTCTTCGCAAGGCTATGAGCAAGTATGGCCAGGTCAAAAACTTGCGCTTGGTCAGGCACATTGTAACTGGTGCTTCGCGCGGATATGCTTTTGTTGAATATGAAACCGAAAGGGAGATGCAGCGTGCATATAAGGAAGCTCACCATTCCTTGATTGATGATTCTAAAGTTATCGTTGATTACAACAGACAGCAGTTGATGCCAGGGTGGATTCCAAGAAGATTAGGAGGTGGTTTCTGTGGTAAGAAGGAATCTGGACAGCTTCGTTTTGGAGGACGAGAAAAACCATTTCGAGCTCCCCTGCGTCCAATCCCATATGACGAGCTGGAGAGACTTGGGATCCCACCTCCACCAGAAGGAAGATACATGTCACCCTATCAGGTCCCGTCCCCACCTAGAAGGAAAGGGAGCTCCATGGATAGAAAAGAAAGTGCCCATGAAAGGTCCATGGACAGGGAAGCGAACATGTGCAGAAGTAGTCCTGTGGAGAACGAAGAAAGGTTTTACAAAAGATCTATTGACCCAGAGGACAGTTCTGTAAGGAGCTCAACTGAGACAGAAGAACACTATCACAACAGGAGCTCGGTTGACAGAGCAGAGCGCTCTCACAGGAGCTCTAGGGATAGGGATGAGCGTGCTTCCAAGAGGCACAAGTCCCGTCACCGTGAGAGGTCCACCGGCCGTGATCGCTACTAA

Protein Analysis

313

Amino Acids

35.79

Weight (kDa)

9.47

Isoelectric Point (pI)

68.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 69 - 137 1e-17 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 92
AccI GTMKAC 1 cut(s) 123
AccII CGCG 4 cut(s) 41, 53, 322, 324
AciI CCGC 6 cut(s) 12, 41, 53, 77, 147, 324
AclWI GGATC 2 cut(s) 577, 590
AcoI YGGCCR 2 cut(s) 271, 922
AfaI GTAC 1 cut(s) 202
AfeI AGCGCT 1 cut(s) 845
AfiI CCNNNNNNNGG 4 cut(s) 169, 599, 684, 696
AflIII ACRYGT 2 cut(s) 609, 708
AgsI TTSAA 1 cut(s) 341
AjnI CCWGG 3 cut(s) 165, 273, 445
AluBI AGCT 8 cut(s) 383, 505, 539, 568, 654, 789, 825, 858
AluI AGCT 8 cut(s) 383, 505, 539, 568, 654, 789, 825, 858
Alw21I GWGCWC 5 cut(s) 541, 656, 791, 827, 860
Alw26I GTCTC 2 cut(s) 568, 791
AlwI GGATC 2 cut(s) 577, 590
Aor51HI AGCGCT 1 cut(s) 845
AoxI GGCC 3 cut(s) 151, 271, 922
ApeKI GCWGC 1 cut(s) 364
AspLEI GCGC 3 cut(s) 291, 324, 846
AspS9I GGNCC 4 cut(s) 172, 625, 687, 915
AsuHPI GGTGA 4 cut(s) 209, 377, 606, 896
AvaII GGWCC 4 cut(s) 172, 625, 687, 915
BaeGI GKGCMC 1 cut(s) 679
BalI TGGCCA 1 cut(s) 273
BamHI GGATCC 1 cut(s) 582
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 5 cut(s) 541, 656, 791, 827, 860
Bbv12I GWGCWC 5 cut(s) 541, 656, 791, 827, 860
BbvI GCAGC 1 cut(s) 376
BceAI ACGGC 3 cut(s) 104, 106, 909
BciT130I CCWGG 3 cut(s) 167, 275, 447
BcoDI GTCTC 2 cut(s) 568, 791
BfaI CTAG 2 cut(s) 639, 861
BfoI RGCGCY 1 cut(s) 847
BglII AGATCT 1 cut(s) 755
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 3 cut(s) 167, 275, 447
Bme18I GGWCC 4 cut(s) 172, 625, 687, 915
BmgT120I GGNCC 4 cut(s) 172, 625, 687, 915
BmiI GGNNCC 5 cut(s) 94, 173, 174, 584, 627
BmrFI CCNGG 3 cut(s) 167, 275, 447
BmsI GCATC 2 cut(s) 351, 432
BplI GAGNNNNNCTC 2 cut(s) 120, 152
BpmI CTGGAG 1 cut(s) 590
BsaJI CCNNGG 6 cut(s) 165, 166, 184, 446, 657, 690
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 599, 684, 696
Bse118I RCCGGY 1 cut(s) 920
Bse1I ACTGG 1 cut(s) 316
BseBI CCWGG 3 cut(s) 167, 275, 447
BseDI CCNNGG 6 cut(s) 165, 166, 184, 446, 657, 690
BseGI GGATG 2 cut(s) 64, 877
BseLI CCNNNNNNNGG 4 cut(s) 169, 599, 684, 696
BseMII CTCAG 1 cut(s) 786
BseNI ACTGG 1 cut(s) 316
BseRI GAGGAG 2 cut(s) 133, 173
BseSI GKGCMC 1 cut(s) 679
BseX3I CGGCCG 1 cut(s) 922
BseXI GCAGC 1 cut(s) 376
BsgI GTGCAG 1 cut(s) 733
Bsh1236I CGCG 4 cut(s) 41, 53, 322, 324
Bsh1285I CGRYCG 1 cut(s) 925
BshFI GGCC 3 cut(s) 153, 273, 924
BshNI GGYRCC 1 cut(s) 92
BsiEI CGRYCG 1 cut(s) 925
BsiHKAI GWGCWC 5 cut(s) 541, 656, 791, 827, 860
BsiSI CCGG 1 cut(s) 921
BslFI GGGAC 4 cut(s) 185, 611, 616, 883
BslI CCNNNNNNNGG 4 cut(s) 169, 599, 684, 696
BsmAI GTCTC 2 cut(s) 568, 791
BsmFI GGGAC 4 cut(s) 185, 611, 616, 883
BsnI GGCC 3 cut(s) 153, 273, 924
Bsp1286I GDGCHC 6 cut(s) 541, 656, 679, 791, 827, 860
Bsp143I GATC 3 cut(s) 582, 755, 928
Bsp19I CCATGG 2 cut(s) 657, 690
BspACI CCGC 6 cut(s) 12, 41, 53, 77, 147, 324
BspANI GGCC 3 cut(s) 153, 273, 924
BspCNI CTCAG 1 cut(s) 787
BspFNI CGCG 4 cut(s) 41, 53, 322, 324
BspLI GGNNCC 5 cut(s) 94, 173, 174, 584, 627
BspPI GGATC 2 cut(s) 577, 590
BspT107I GGYRCC 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 920
BsrI ACTGG 1 cut(s) 316
BssAI RCCGGY 1 cut(s) 920
BssECI CCNNGG 6 cut(s) 165, 166, 184, 446, 657, 690
BssMI GATC 3 cut(s) 582, 755, 928
BssT1I CCWWGG 2 cut(s) 657, 690
Bst2UI CCWGG 3 cut(s) 167, 275, 447
Bst4CI ACNGT 2 cut(s) 776, 908
Bst6I CTCTTC 2 cut(s) 212, 252
BstAPI GCANNNNNTGC 1 cut(s) 442
BstC8I GCNNGC 3 cut(s) 151, 369, 879
BstDEI CTNAG 1 cut(s) 795
BstDSI CCRYGG 2 cut(s) 657, 690
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 2 cut(s) 64, 877
BstFNI CGCG 4 cut(s) 41, 53, 322, 324
BstH2I RGCGCY 1 cut(s) 847
BstHHI GCGC 3 cut(s) 291, 324, 846
BstKTI GATC 3 cut(s) 585, 758, 931
BstMAI GTCTC 2 cut(s) 568, 791
BstMBI GATC 3 cut(s) 582, 755, 928
BstMCI CGRYCG 1 cut(s) 925
BstMWI GCNNNNNNNGC 5 cut(s) 90, 249, 270, 442, 930
BstNI CCWGG 3 cut(s) 167, 275, 447
BstNSI RCATGY 2 cut(s) 613, 712
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 3 cut(s) 165, 273, 445
BstSLI GKGCMC 1 cut(s) 679
BstUI CGCG 4 cut(s) 41, 53, 322, 324
BstV1I GCAGC 1 cut(s) 376
BstX2I RGATCY 2 cut(s) 582, 755
BstYI RGATCY 2 cut(s) 582, 755
BstZI CGGCCG 1 cut(s) 922
BsuRI GGCC 3 cut(s) 153, 273, 924
BtgI CCRYGG 2 cut(s) 657, 690
BtsCI GGATG 2 cut(s) 64, 877
Cac8I GCNNGC 3 cut(s) 151, 369, 879
CfoI GCGC 3 cut(s) 291, 324, 846
Cfr10I RCCGGY 1 cut(s) 920
Cfr13I GGNCC 4 cut(s) 172, 625, 687, 915
CseI GACGC 1 cut(s) 536
Csp6I GTAC 1 cut(s) 201
CviAII CATG 5 cut(s) 610, 658, 680, 691, 709
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 1 cut(s) 795
DpnI GATC 3 cut(s) 584, 757, 930
DpnII GATC 3 cut(s) 582, 755, 928
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 2 cut(s) 271, 922
EagI CGGCCG 1 cut(s) 922
Eam1104I CTCTTC 2 cut(s) 212, 252
EarI CTCTTC 2 cut(s) 212, 252
EciI GGCGGA 1 cut(s) 27
Ecl136II GAGCTC 5 cut(s) 539, 654, 789, 825, 858
EclXI CGGCCG 1 cut(s) 922
Eco130I CCWWGG 2 cut(s) 657, 690
Eco24I GRGCYC 5 cut(s) 541, 656, 791, 827, 860
Eco47I GGWCC 4 cut(s) 172, 625, 687, 915
Eco47III AGCGCT 1 cut(s) 845
Eco52I CGGCCG 1 cut(s) 922
Eco53kI GAGCTC 5 cut(s) 539, 654, 789, 825, 858
Eco91I GGTNACC 1 cut(s) 215
EcoICRI GAGCTC 5 cut(s) 539, 654, 789, 825, 858
EcoO109I RGGNCCY 2 cut(s) 172, 625
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 3 cut(s) 165, 273, 445
EcoT14I CCWWGG 2 cut(s) 657, 690
EcoT38I GRGCYC 5 cut(s) 541, 656, 791, 827, 860
ErhI CCWWGG 2 cut(s) 657, 690
FaeI CATG 5 cut(s) 613, 661, 683, 694, 712
FaqI GGGAC 4 cut(s) 185, 611, 616, 883
FatI CATG 5 cut(s) 609, 657, 679, 690, 708
FauI CCCGC 1 cut(s) 70
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 365
FokI GGATG 2 cut(s) 51, 884
FriOI GRGCYC 5 cut(s) 541, 656, 791, 827, 860
Fsp4HI GCNGC 1 cut(s) 365
FspBI CTAG 2 cut(s) 639, 861
GlaI GCGC 3 cut(s) 290, 323, 845
GluI GCNGC 1 cut(s) 365
GsuI CTGGAG 1 cut(s) 590
HaeII RGCGCY 1 cut(s) 847
HaeIII GGCC 3 cut(s) 153, 273, 924
HapII CCGG 1 cut(s) 921
HgaI GACGC 1 cut(s) 536
HhaI GCGC 3 cut(s) 291, 324, 846
Hin1II CATG 5 cut(s) 613, 661, 683, 694, 712
Hin6I GCGC 3 cut(s) 289, 322, 844
HinP1I GCGC 3 cut(s) 289, 322, 844
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HinfI GANTC 4 cut(s) 70, 404, 454, 494
HpaII CCGG 1 cut(s) 921
HphI GGTGA 4 cut(s) 209, 377, 606, 896
Hpy166II GTNNAC 4 cut(s) 25, 124, 832, 918
Hpy188I TCNGA 1 cut(s) 69
Hpy188III TCNNGA 2 cut(s) 110, 498
Hpy8I GTNNAC 4 cut(s) 25, 124, 832, 918
HpyAV CCTTC 4 cut(s) 142, 484, 593, 636
HpyCH4III ACNGT 2 cut(s) 776, 908
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 3 cut(s) 364, 371, 714
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 249, 270, 442, 930
HpyF3I CTNAG 1 cut(s) 795
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 5 cut(s) 613, 661, 683, 694, 712
HspAI GCGC 3 cut(s) 289, 322, 844
KflI GGGWCCC 1 cut(s) 172
Kzo9I GATC 3 cut(s) 582, 755, 928
LmnI GCTCC 7 cut(s) 146, 544, 651, 659, 786, 822, 855
Lsp1109I GCAGC 1 cut(s) 376
LweI GCATC 2 cut(s) 351, 432
MaeI CTAG 2 cut(s) 639, 861
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 4 cut(s) 215, 307, 612, 902
MalI GATC 3 cut(s) 584, 757, 930
MboI GATC 3 cut(s) 582, 755, 928
MboII GAAGA 6 cut(s) 199, 239, 474, 615, 749, 815
MflI RGATCY 2 cut(s) 582, 755
MhlI GDGCHC 6 cut(s) 541, 656, 679, 791, 827, 860
MlsI TGGCCA 1 cut(s) 273
MluNI TGGCCA 1 cut(s) 273
Mox20I TGGCCA 1 cut(s) 273
MscI TGGCCA 1 cut(s) 273
MseI TTAA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 1 cut(s) 921
MspR9I CCNGG 3 cut(s) 167, 275, 447
MvaI CCWGG 3 cut(s) 167, 275, 447
MvnI CGCG 4 cut(s) 41, 53, 322, 324
MwoI GCNNNNNNNGC 5 cut(s) 90, 249, 270, 442, 930
NcoI CCATGG 2 cut(s) 657, 690
NdeI CATATG 1 cut(s) 559
NdeII GATC 3 cut(s) 582, 755, 928
NlaIII CATG 5 cut(s) 613, 661, 683, 694, 712
NlaIV GGNNCC 5 cut(s) 94, 173, 174, 584, 627
NmeAIII GCCGAG 1 cut(s) 165
NmuCI GTSAC 3 cut(s) 215, 612, 902
NspI RCATGY 2 cut(s) 613, 712
PasI CCCWGGG 1 cut(s) 166
PciI ACATGT 2 cut(s) 609, 708
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 4 cut(s) 70, 404, 454, 494
PkrI GCNGC 1 cut(s) 366
PpuMI RGGWCCY 2 cut(s) 172, 625
PscI ACATGT 2 cut(s) 609, 708
Psp124BI GAGCTC 5 cut(s) 541, 656, 791, 827, 860
Psp5II RGGWCCY 2 cut(s) 172, 625
Psp6I CCWGG 3 cut(s) 165, 273, 445
PspEI GGTNACC 1 cut(s) 215
PspGI CCWGG 3 cut(s) 165, 273, 445
PspN4I GGNNCC 5 cut(s) 94, 173, 174, 584, 627
PspPI GGNCC 4 cut(s) 172, 625, 687, 915
PspPPI RGGWCCY 2 cut(s) 172, 625
PsuI RGATCY 2 cut(s) 582, 755
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
SacI GAGCTC 5 cut(s) 541, 656, 791, 827, 860
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 365
Sau3AI GATC 3 cut(s) 582, 755, 928
Sau96I GGNCC 4 cut(s) 172, 625, 687, 915
ScrFI CCNGG 3 cut(s) 167, 275, 447
SduI GDGCHC 6 cut(s) 541, 656, 679, 791, 827, 860
SfaNI GCATC 2 cut(s) 351, 432
SinI GGWCC 4 cut(s) 172, 625, 687, 915
SsiI CCGC 6 cut(s) 12, 41, 53, 77, 147, 324
SspMI CTAG 2 cut(s) 639, 861
SstI GAGCTC 5 cut(s) 541, 656, 791, 827, 860
StyD4I CCNGG 3 cut(s) 165, 273, 445
StyI CCWWGG 2 cut(s) 657, 690
TaaI ACNGT 2 cut(s) 776, 908
TaiI ACGT 1 cut(s) 24
TaqI TCGA 1 cut(s) 535
TatI WGTACW 1 cut(s) 200
TfiI GAWTC 4 cut(s) 70, 404, 454, 494
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TseFI GTSAC 3 cut(s) 215, 612, 902
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 3 cut(s) 215, 612, 902
TspDTI ATGAA 2 cut(s) 360, 696
TspGWI ACGGA 1 cut(s) 42
VpaK11BI GGWCC 4 cut(s) 172, 625, 687, 915
XceI RCATGY 2 cut(s) 613, 712
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 2 cut(s) 639, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.