RchiOBHm_Chr4g0412221

U11 U12 small nuclear ribonucleoprotein 35 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009123
Physical Location & Seq
Forward (+)
35967952 .. 35972895
4944 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38294

Sequence Viewer

Length: 1092 bp
ATGAGGAAGACAGAGGCAGTGTTCTACGCGGATACCTACCATCCGATCCAAGCCGGAAGCATCGACGGCACGGACACTCTCCCCCACGACAGCGCCGTCTATCGCGCCTGGCTTTGCTCCTCCGCCGCCCTCTATGACCCCTCCGGCGATCCCAAGCTCATCGGCGACCCCTATTTCACTCTCTTCGTCGCCCGCCTCTCTCCCCTCAGCACCGAAAACACTCTTCGTCAGGCTATGAGCAAGTATGGTCGGGTTAAGAACTTGCGTTTGGTCAGGCATATTGTAACCGGTGCCTCGCGTGGCTATGCTTTCGTTGAGTATGAAACCGAAAGGGAGATGCGGCGTGCATACAAGGAAGCTCACCATTCCCTTGTTGATGATTATGAAATTATCGTGGATTACAACAGACAGCAGTTGATGCCAGGATGGATTCCAAGACGATTAGGAGGGGGTTTCGGTGGTAAGAAGGAATCTGGACAACTTCGATTTGGAGGACGAGAAAGACCATTTCGAGCTCCCATTCGTATCCCATATGATGACCTGGAGAAACTTGGAATCCCACCTCCACCAGAAGGGCGATACATGTCCCGCTATCAGGACCCATCCCCACCTAGAAGAAAAAGAAGCTCAGTAGATAGGGAAGAAGGTACTCACAGAAGGTCAATGGATAGGGAAGCGAACATACACAAAAGCAATCCTGCAGATAACGATGACAGGTTTCACAAAAGATCTATGGATGGGGACTACCATTCATCCAGAAGGAGCTCTGATGAGTCAGCTCAACGCCATCGCAGTAGGAGCTCTAGAGATAGAGAAGAAGGTACTCACAGAAGGTCTACTGATAGGGAAGCAAGCATACCCAAAAGCAGTCCTGTCGACAAGGAAGAGTCGTTTCGTAAAAGATCTATGGACCAGGAGGGCCGTTCATTCAAAAGGAGCTCTGTTGAGACCGAAGAATTCCATCGCAGCAGGAGATCTGTGGACATGGAAGAGCGTTCACACAAAAGGAGCTCTAGGGATAGGGATGAGCGTTCTCACAAGCGGCACCAGTCCCGTCACTATGAGAGGTCTACTAGTCATGATCGCTCTTAA

Protein Analysis

363

Amino Acids

42.45

Weight (kDa)

9.8

Isoelectric Point (pI)

87.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 61 - 130 2.8e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 290, 1044
AccI GTMKAC 3 cut(s) 836, 876, 1070
AccII CGCG 3 cut(s) 29, 105, 298
AciI CCGC 7 cut(s) 29, 123, 126, 193, 340, 589, 1042
AclWI GGATC 2 cut(s) 40, 143
AcsI RAATTY 1 cut(s) 956
AfaI GTAC 2 cut(s) 649, 823
AfiI CCNNNNNNNGG 2 cut(s) 572, 595
AflIII ACRYGT 1 cut(s) 582
AgeI ACCGGT 1 cut(s) 287
AgsI TTSAA 1 cut(s) 931
AhlI ACTAGT 1 cut(s) 1073
AjnI CCWGG 4 cut(s) 107, 421, 540, 912
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 9 cut(s) 157, 359, 515, 627, 765, 779, 801, 939, 1011
AluI AGCT 9 cut(s) 157, 359, 515, 627, 765, 779, 801, 939, 1011
Alw21I GWGCWC 5 cut(s) 517, 767, 803, 941, 1013
Alw26I GTCTC 1 cut(s) 941
AlwI GGATC 2 cut(s) 40, 143
AoxI GGCC 1 cut(s) 919
ApeKI GCWGC 1 cut(s) 966
ApoI RAATTY 1 cut(s) 956
AsiGI ACCGGT 1 cut(s) 287
AspLEI GCGC 2 cut(s) 95, 107
AspS9I GGNCC 3 cut(s) 598, 910, 919
AsuHPI GGTGA 1 cut(s) 353
AvaII GGWCC 2 cut(s) 598, 910
BanI GGYRCC 2 cut(s) 290, 1044
BanII GRGCYC 5 cut(s) 517, 767, 803, 941, 1013
BarI GAAGNNNNNNTAC 4 cut(s) 666, 698, 840, 872
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 5 cut(s) 517, 767, 803, 941, 1013
BbvCI CCTCAGC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 978
BccI CCATC 6 cut(s) 48, 420, 610, 731, 795, 969
BceAI ACGGC 3 cut(s) 80, 82, 906
BcgI CGANNNNNNTGC 2 cut(s) 856, 890
BciT130I CCWGG 4 cut(s) 109, 423, 542, 914
BciVI GTATCC 2 cut(s) 25, 536
BcoDI GTCTC 1 cut(s) 941
BcuI ACTAGT 1 cut(s) 1073
BfaI CTAG 4 cut(s) 612, 804, 1014, 1074
BfmI CTRYAG 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 96
BfuI GTATCC 2 cut(s) 25, 536
BglII AGATCT 3 cut(s) 728, 902, 974
BisI GCNGC 4 cut(s) 126, 341, 967, 1043
BlsI GCNGC 4 cut(s) 127, 342, 968, 1044
Bme1390I CCNGG 4 cut(s) 109, 423, 542, 914
Bme18I GGWCC 2 cut(s) 598, 910
BmgT120I GGNCC 3 cut(s) 598, 910, 919
BmiI GGNNCC 3 cut(s) 292, 600, 1046
BmrFI CCNGG 4 cut(s) 109, 423, 542, 914
BmsI GCATC 3 cut(s) 69, 327, 408
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 206
BsaI GGTCTC 1 cut(s) 941
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 2 cut(s) 572, 595
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 1 cut(s) 1048
BseBI CCWGG 4 cut(s) 109, 423, 542, 914
BseGI GGATG 6 cut(s) 40, 431, 602, 742, 752, 1030
BseLI CCNNNNNNNGG 2 cut(s) 572, 595
BseMII CTCAG 2 cut(s) 220, 642
BseNI ACTGG 1 cut(s) 1048
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 978
Bsh1236I CGCG 3 cut(s) 29, 105, 298
BshFI GGCC 1 cut(s) 921
BshNI GGYRCC 2 cut(s) 290, 1044
BshTI ACCGGT 1 cut(s) 287
BsiHKAI GWGCWC 5 cut(s) 517, 767, 803, 941, 1013
BsiSI CCGG 3 cut(s) 54, 144, 288
BslFI GGGAC 3 cut(s) 571, 755, 1036
BslI CCNNNNNNNGG 2 cut(s) 572, 595
BsmAI GTCTC 1 cut(s) 941
BsmFI GGGAC 3 cut(s) 571, 755, 1036
BsnI GGCC 1 cut(s) 921
Bso31I GGTCTC 1 cut(s) 941
Bsp1286I GDGCHC 5 cut(s) 517, 767, 803, 941, 1013
Bsp143I GATC 6 cut(s) 45, 148, 728, 902, 974, 1081
BspACI CCGC 7 cut(s) 29, 123, 126, 193, 340, 589, 1042
BspANI GGCC 1 cut(s) 921
BspCNI CTCAG 2 cut(s) 219, 641
BspFNI CGCG 3 cut(s) 29, 105, 298
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 3 cut(s) 292, 600, 1046
BspMAI CTGCAG 1 cut(s) 703
BspPI GGATC 2 cut(s) 40, 143
BspQI GCTCTTC 1 cut(s) 984
BspT107I GGYRCC 2 cut(s) 290, 1044
BspTNI GGTCTC 1 cut(s) 941
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 1 cut(s) 1048
BssAI RCCGGY 1 cut(s) 287
BssMI GATC 6 cut(s) 45, 148, 728, 902, 974, 1081
Bst2UI CCWGG 4 cut(s) 109, 423, 542, 914
Bst6I CTCTTC 4 cut(s) 188, 228, 879, 984
BstAPI GCANNNNNTGC 1 cut(s) 418
BstC8I GCNNGC 3 cut(s) 193, 345, 853
BstDEI CTNAG 2 cut(s) 206, 628
BstF5I GGATG 6 cut(s) 40, 431, 602, 742, 752, 1030
BstFNI CGCG 3 cut(s) 29, 105, 298
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 2 cut(s) 95, 107
BstKTI GATC 6 cut(s) 48, 151, 731, 905, 977, 1084
BstMAI GTCTC 1 cut(s) 941
BstMBI GATC 6 cut(s) 45, 148, 728, 902, 974, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 66, 418, 798
BstNI CCWGG 4 cut(s) 109, 423, 542, 914
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 4 cut(s) 107, 421, 540, 912
BstSFI CTRYAG 1 cut(s) 699
BstUI CGCG 3 cut(s) 29, 105, 298
BstV1I GCAGC 1 cut(s) 978
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 3 cut(s) 728, 902, 974
BstYI RGATCY 3 cut(s) 728, 902, 974
BsuI GTATCC 2 cut(s) 25, 536
BsuRI GGCC 1 cut(s) 921
BtgZI GCGATG 2 cut(s) 773, 947
BtsCI GGATG 6 cut(s) 40, 431, 602, 742, 752, 1030
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 3 cut(s) 193, 345, 853
CciI TCATGA 1 cut(s) 1078
CfoI GCGC 2 cut(s) 95, 107
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 3 cut(s) 598, 910, 919
Csp6I GTAC 2 cut(s) 648, 822
CspAI ACCGGT 1 cut(s) 287
CviAII CATG 3 cut(s) 583, 985, 1079
CviQI GTAC 2 cut(s) 648, 822
DdeI CTNAG 2 cut(s) 206, 628
DpnI GATC 6 cut(s) 47, 150, 730, 904, 976, 1083
DpnII GATC 6 cut(s) 45, 148, 728, 902, 974, 1081
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 4 cut(s) 188, 228, 879, 984
EarI CTCTTC 4 cut(s) 188, 228, 879, 984
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 5 cut(s) 515, 765, 801, 939, 1011
Eco24I GRGCYC 5 cut(s) 517, 767, 803, 941, 1013
Eco31I GGTCTC 1 cut(s) 941
Eco47I GGWCC 2 cut(s) 598, 910
Eco53kI GAGCTC 5 cut(s) 515, 765, 801, 939, 1011
EcoICRI GAGCTC 5 cut(s) 515, 765, 801, 939, 1011
EcoO109I RGGNCCY 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 956
EcoRII CCWGG 4 cut(s) 107, 421, 540, 912
EcoT38I GRGCYC 5 cut(s) 517, 767, 803, 941, 1013
FaeI CATG 3 cut(s) 586, 988, 1082
FaqI GGGAC 3 cut(s) 571, 755, 1036
FatI CATG 3 cut(s) 582, 984, 1078
FauI CCCGC 2 cut(s) 200, 596
FauNDI CATATG 1 cut(s) 532
FblI GTMKAC 3 cut(s) 836, 876, 1070
Fnu4HI GCNGC 4 cut(s) 126, 341, 967, 1043
FokI GGATG 6 cut(s) 27, 438, 589, 739, 749, 1037
FriOI GRGCYC 5 cut(s) 517, 767, 803, 941, 1013
Fsp4HI GCNGC 4 cut(s) 126, 341, 967, 1043
FspBI CTAG 4 cut(s) 612, 804, 1014, 1074
GlaI GCGC 2 cut(s) 94, 106
GluI GCNGC 4 cut(s) 126, 341, 967, 1043
GsuI CTGGAG 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 1 cut(s) 921
HapII CCGG 3 cut(s) 54, 144, 288
HhaI GCGC 2 cut(s) 95, 107
Hin1II CATG 3 cut(s) 586, 988, 1082
Hin6I GCGC 2 cut(s) 93, 105
HinP1I GCGC 2 cut(s) 93, 105
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 5 cut(s) 430, 470, 555, 773, 887
HpaII CCGG 3 cut(s) 54, 144, 288
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 5 cut(s) 837, 877, 982, 998, 1071
Hpy188I TCNGA 2 cut(s) 45, 769
Hpy188III TCNNGA 5 cut(s) 474, 596, 756, 804, 1079
Hpy8I GTNNAC 5 cut(s) 837, 877, 982, 998, 1071
Hpy99I CGWCG 2 cut(s) 68, 191
HpyAV CCTTC 7 cut(s) 460, 566, 638, 651, 753, 812, 825
HpyCH4V TGCA 2 cut(s) 347, 701
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 418, 798
HpyF3I CTNAG 2 cut(s) 206, 628
Hsp92II CATG 3 cut(s) 586, 988, 1082
HspAI GCGC 2 cut(s) 93, 105
Kzo9I GATC 6 cut(s) 45, 148, 728, 902, 974, 1081
LguI GCTCTTC 1 cut(s) 984
LmnI GCTCC 6 cut(s) 122, 520, 762, 798, 936, 1008
Lsp1109I GCAGC 1 cut(s) 978
LweI GCATC 3 cut(s) 69, 327, 408
MaeI CTAG 4 cut(s) 612, 804, 1014, 1074
MaeIII GTNAC 2 cut(s) 283, 1055
MalI GATC 6 cut(s) 47, 150, 730, 904, 976, 1083
MboI GATC 6 cut(s) 45, 148, 728, 902, 974, 1081
MboII GAAGA 9 cut(s) 19, 175, 215, 627, 653, 827, 896, 965, 1001
MflI RGATCY 3 cut(s) 728, 902, 974
MhlI GDGCHC 5 cut(s) 517, 767, 803, 941, 1013
MluCI AATT 2 cut(s) 387, 956
MlyI GAGTC 2 cut(s) 782, 896
MseI TTAA 2 cut(s) 255, 1090
MspI CCGG 3 cut(s) 54, 144, 288
MspR9I CCNGG 4 cut(s) 109, 423, 542, 914
MvaI CCWGG 4 cut(s) 109, 423, 542, 914
MvnI CGCG 3 cut(s) 29, 105, 298
MwoI GCNNNNNNNGC 3 cut(s) 66, 418, 798
NdeI CATATG 1 cut(s) 532
NdeII GATC 6 cut(s) 45, 148, 728, 902, 974, 1081
NlaIII CATG 3 cut(s) 586, 988, 1082
NlaIV GGNNCC 3 cut(s) 292, 600, 1046
NmuCI GTSAC 1 cut(s) 1055
NspI RCATGY 1 cut(s) 586
PagI TCATGA 1 cut(s) 1078
PciI ACATGT 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 984
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 3 cut(s) 430, 470, 555
PinAI ACCGGT 1 cut(s) 287
PkrI GCNGC 4 cut(s) 127, 342, 968, 1044
PleI GAGTC 2 cut(s) 781, 895
PpsI GAGTC 2 cut(s) 781, 895
PpuMI RGGWCCY 1 cut(s) 598
PscI ACATGT 1 cut(s) 582
Psp124BI GAGCTC 5 cut(s) 517, 767, 803, 941, 1013
Psp5II RGGWCCY 1 cut(s) 598
Psp6I CCWGG 4 cut(s) 107, 421, 540, 912
PspGI CCWGG 4 cut(s) 107, 421, 540, 912
PspN4I GGNNCC 3 cut(s) 292, 600, 1046
PspPI GGNCC 3 cut(s) 598, 910, 919
PspPPI RGGWCCY 1 cut(s) 598
PstI CTGCAG 1 cut(s) 703
PsuI RGATCY 3 cut(s) 728, 902, 974
RsaI GTAC 2 cut(s) 649, 823
RsaNI GTAC 2 cut(s) 648, 822
SacI GAGCTC 5 cut(s) 517, 767, 803, 941, 1013
SalI GTCGAC 1 cut(s) 875
SapI GCTCTTC 1 cut(s) 984
SaqAI TTAA 2 cut(s) 255, 1090
SatI GCNGC 4 cut(s) 126, 341, 967, 1043
Sau3AI GATC 6 cut(s) 45, 148, 728, 902, 974, 1081
Sau96I GGNCC 3 cut(s) 598, 910, 919
SchI GAGTC 2 cut(s) 782, 896
ScrFI CCNGG 4 cut(s) 109, 423, 542, 914
SduI GDGCHC 5 cut(s) 517, 767, 803, 941, 1013
SfaNI GCATC 3 cut(s) 69, 327, 408
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 2 cut(s) 598, 910
SpeI ACTAGT 1 cut(s) 1073
Sse9I AATT 2 cut(s) 387, 956
SsiI CCGC 7 cut(s) 29, 123, 126, 193, 340, 589, 1042
SspMI CTAG 4 cut(s) 612, 804, 1014, 1074
SstI GAGCTC 5 cut(s) 517, 767, 803, 941, 1013
StyD4I CCNGG 4 cut(s) 107, 421, 540, 912
TaqI TCGA 4 cut(s) 63, 484, 511, 876
TaqII GACCGA 1 cut(s) 965
TasI AATT 2 cut(s) 387, 956
TauI GCSGC 3 cut(s) 128, 343, 1045
TfiI GAWTC 3 cut(s) 430, 470, 555
Tru1I TTAA 2 cut(s) 255, 1090
Tru9I TTAA 2 cut(s) 255, 1090
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 1055
TseI GCWGC 1 cut(s) 966
Tsp45I GTSAC 1 cut(s) 1055
TspDTI ATGAA 4 cut(s) 336, 399, 741, 915
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 1 cut(s) 24
VpaK11BI GGWCC 2 cut(s) 598, 910
XapI RAATTY 1 cut(s) 956
XbaI TCTAGA 1 cut(s) 803
XceI RCATGY 1 cut(s) 586
XmiI GTMKAC 3 cut(s) 836, 876, 1070
XspI CTAG 4 cut(s) 612, 804, 1014, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.