RchiOBHm_Chr4g0412301

U11 U12 small nuclear ribonucleoprotein 35 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009123
Physical Location & Seq
Forward (+)
36024854 .. 36029464
4611 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38298

Sequence Viewer

Length: 1092 bp
ATGAGGAAGACAGGGGCAGTGTTCTACGCGGATACCTACCATCCGATCCAAGCCGGCAGCATCGACGGCACCGACACTCTCCCTCACGACAGCGCCGTCTATCGAGCCTGGCTTTGCTCCTCCGCCGCCCTCTATGACCCGTCCGGCGATCCGAAGCTCATCGGCGACCCCTACCGCACTCTCTTCGTCGCCCGTCTCTCTCCCCTCACCACCGAAAACACTCTTCGCCAGGCTATGAGCAAGTATGGTCGGGTTAAGAACTTGCGTTTGGTCAGGCATATCGTAACCGGTGCCTCGCGTGGCTATGCTTTTGTTGAGTATGAAACCGAAAGGGAGATGCGGCGTGGATACAAGGAAGCTCACCATTCCCTTGTTGATGATTATGAAATTATCGTGGATTACAACAGACAGCAGTTGATGCCAGGATGGATTCCAAGACGATTAGGAGGGGGTTTTGGTGGTAAGAAGGAATCTGGACAACTTCGATTTGGAGGACGAGAAAGACCATTTCGAGCTCCCATTCGTATCCCATATGATGATCTGGAGAAACTTGGAATCCCACCTCCACCAGAAGGGCAATACATGTCCCGGTATCAGGACCCATCCCCACCTAGAAGAAAAAGAAGCTCAGTAGATGGGGAAAAAGGTACTCACAGAAGGTCAATGGATAGGGAAGCGAACATACGCAAAAGCAATCCTGCAGATAATGATGACAGGTTTCATAAAAGATCTATGGATGAGGACTACCATTCACCCAGAAGGAGCTCTAATGAGTCAGCTCAACGCCATCACAGTAGGAGCTCTAGAGATAGAGAAGGAGGTACTCACAGAAGGTCTACTGATAGGGAAGCAAGCATGCCCAAAAGCAGTCCTCTCGACAAGGAAGAGTCGTTTCGTAAAAGTTCTATGGACCAGGAAGACCGTACATTCAAAAGGAGCTCTATTGAGACCGAAGAATTCCATCGCAGTAGGAGATCTGTGGACACGGAGGAGCGTTCACACAAAAGGAGCTCTAGAGATAGGGATGAGCATTCTCACAAGCGGCACAAGTCCCGTCATTATGAGAGGTCCACTAGTTATGATCGCTCTTAA

Protein Analysis

363

Amino Acids

42.29

Weight (kDa)

9.79

Isoelectric Point (pI)

81.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 61 - 130 1.6e-15 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 68, 290
AccI GTMKAC 1 cut(s) 836
AccII CGCG 2 cut(s) 29, 298
AciI CCGC 6 cut(s) 29, 123, 126, 175, 340, 1042
AclWI GGATC 2 cut(s) 40, 143
AcsI RAATTY 1 cut(s) 956
AfaI GTAC 3 cut(s) 649, 823, 925
AfiI CCNNNNNNNGG 2 cut(s) 572, 595
AflIII ACRYGT 1 cut(s) 582
AgeI ACCGGT 1 cut(s) 287
AgsI TTSAA 1 cut(s) 931
AhlI ACTAGT 1 cut(s) 1073
AjnI CCWGG 4 cut(s) 107, 228, 421, 912
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 9 cut(s) 157, 359, 515, 627, 765, 779, 801, 939, 1011
AluI AGCT 9 cut(s) 157, 359, 515, 627, 765, 779, 801, 939, 1011
Alw21I GWGCWC 5 cut(s) 517, 767, 803, 941, 1013
Alw26I GTCTC 2 cut(s) 200, 941
AlwI GGATC 2 cut(s) 40, 143
ApeKI GCWGC 1 cut(s) 57
ApoI RAATTY 1 cut(s) 956
AsiGI ACCGGT 1 cut(s) 287
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 3 cut(s) 598, 910, 1068
AsuC2I CCSGG 1 cut(s) 589
AsuHPI GGTGA 3 cut(s) 199, 353, 744
AvaII GGWCC 3 cut(s) 598, 910, 1068
BanI GGYRCC 2 cut(s) 68, 290
BanII GRGCYC 5 cut(s) 517, 767, 803, 941, 1013
BarI GAAGNNNNNNTAC 2 cut(s) 666, 698
BbsI GAAGAC 2 cut(s) 14, 924
Bbv12I GWGCWC 5 cut(s) 517, 767, 803, 941, 1013
BbvI GCAGC 1 cut(s) 69
BccI CCATC 6 cut(s) 48, 420, 610, 629, 795, 969
BceAI ACGGC 2 cut(s) 80, 82
BcgI CGANNNNNNTGC 4 cut(s) 166, 200, 856, 890
BciT130I CCWGG 4 cut(s) 109, 230, 423, 914
BciVI GTATCC 3 cut(s) 25, 341, 536
BcnI CCSGG 1 cut(s) 589
BcoDI GTCTC 2 cut(s) 200, 941
BcuI ACTAGT 1 cut(s) 1073
BfaI CTAG 4 cut(s) 612, 804, 1014, 1074
BfmI CTRYAG 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 96
BfuI GTATCC 3 cut(s) 25, 341, 536
BglII AGATCT 2 cut(s) 728, 974
BisI GCNGC 4 cut(s) 58, 126, 341, 1043
BlsI GCNGC 4 cut(s) 59, 127, 342, 1044
Bme1390I CCNGG 5 cut(s) 109, 230, 423, 589, 914
Bme18I GGWCC 3 cut(s) 598, 910, 1068
BmgT120I GGNCC 3 cut(s) 598, 910, 1068
BmiI GGNNCC 3 cut(s) 70, 292, 600
BmrFI CCNGG 5 cut(s) 109, 230, 423, 589, 914
BmsI GCATC 3 cut(s) 69, 327, 408
BpiI GAAGAC 2 cut(s) 14, 924
BpmI CTGGAG 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 589
BsaI GGTCTC 1 cut(s) 941
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 2 cut(s) 572, 595
Bse118I RCCGGY 2 cut(s) 53, 287
BseBI CCWGG 4 cut(s) 109, 230, 423, 914
BseGI GGATG 5 cut(s) 40, 431, 602, 742, 1030
BseLI CCNNNNNNNGG 2 cut(s) 572, 595
BseMII CTCAG 1 cut(s) 642
BseRI GAGGAG 2 cut(s) 109, 1004
BseXI GCAGC 1 cut(s) 69
Bsh1236I CGCG 2 cut(s) 29, 298
BshNI GGYRCC 2 cut(s) 68, 290
BshTI ACCGGT 1 cut(s) 287
BsiHKAI GWGCWC 5 cut(s) 517, 767, 803, 941, 1013
BsiSI CCGG 4 cut(s) 54, 144, 288, 589
BslFI GGGAC 2 cut(s) 571, 1036
BslI CCNNNNNNNGG 2 cut(s) 572, 595
BsmAI GTCTC 2 cut(s) 200, 941
BsmBI CGTCTC 1 cut(s) 200
BsmFI GGGAC 2 cut(s) 571, 1036
BsmI GAATGC 1 cut(s) 1030
Bso31I GGTCTC 1 cut(s) 941
Bsp1286I GDGCHC 5 cut(s) 517, 767, 803, 941, 1013
Bsp143I GATC 6 cut(s) 45, 148, 538, 728, 974, 1081
BspACI CCGC 6 cut(s) 29, 123, 126, 175, 340, 1042
BspCNI CTCAG 1 cut(s) 641
BspFNI CGCG 2 cut(s) 29, 298
BspLI GGNNCC 3 cut(s) 70, 292, 600
BspMAI CTGCAG 1 cut(s) 703
BspPI GGATC 2 cut(s) 40, 143
BspT107I GGYRCC 2 cut(s) 68, 290
BspTNI GGTCTC 1 cut(s) 941
BsrFI RCCGGY 2 cut(s) 53, 287
BssAI RCCGGY 2 cut(s) 53, 287
BssMI GATC 6 cut(s) 45, 148, 538, 728, 974, 1081
Bst2UI CCWGG 4 cut(s) 109, 230, 423, 914
Bst4CI ACNGT 2 cut(s) 794, 923
Bst6I CTCTTC 3 cut(s) 188, 228, 879
BstAPI GCANNNNNTGC 1 cut(s) 418
BstC8I GCNNGC 3 cut(s) 55, 853, 857
BstDEI CTNAG 1 cut(s) 628
BstF5I GGATG 5 cut(s) 40, 431, 602, 742, 1030
BstFNI CGCG 2 cut(s) 29, 298
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 6 cut(s) 48, 151, 541, 731, 977, 1084
BstMAI GTCTC 2 cut(s) 200, 941
BstMBI GATC 6 cut(s) 45, 148, 538, 728, 974, 1081
BstMWI GCNNNNNNNGC 2 cut(s) 66, 418
BstNI CCWGG 4 cut(s) 109, 230, 423, 914
BstNSI RCATGY 2 cut(s) 586, 859
BstSCI CCNGG 5 cut(s) 107, 228, 421, 587, 912
BstSFI CTRYAG 1 cut(s) 699
BstUI CGCG 2 cut(s) 29, 298
BstV1I GCAGC 1 cut(s) 69
BstV2I GAAGAC 2 cut(s) 14, 924
BstX2I RGATCY 2 cut(s) 728, 974
BstYI RGATCY 2 cut(s) 728, 974
BsuI GTATCC 3 cut(s) 25, 341, 536
BtgZI GCGATG 1 cut(s) 947
BtsCI GGATG 5 cut(s) 40, 431, 602, 742, 1030
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 3 cut(s) 55, 853, 857
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 2 cut(s) 53, 287
Cfr13I GGNCC 3 cut(s) 598, 910, 1068
Csp6I GTAC 3 cut(s) 648, 822, 924
CspAI ACCGGT 1 cut(s) 287
CviAII CATG 2 cut(s) 583, 856
CviQI GTAC 3 cut(s) 648, 822, 924
DdeI CTNAG 1 cut(s) 628
DpnI GATC 6 cut(s) 47, 150, 540, 730, 976, 1083
DpnII GATC 6 cut(s) 45, 148, 538, 728, 974, 1081
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 3 cut(s) 188, 228, 879
EarI CTCTTC 3 cut(s) 188, 228, 879
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 5 cut(s) 515, 765, 801, 939, 1011
Eco24I GRGCYC 5 cut(s) 517, 767, 803, 941, 1013
Eco31I GGTCTC 1 cut(s) 941
Eco47I GGWCC 3 cut(s) 598, 910, 1068
Eco53kI GAGCTC 5 cut(s) 515, 765, 801, 939, 1011
EcoICRI GAGCTC 5 cut(s) 515, 765, 801, 939, 1011
EcoO109I RGGNCCY 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 956
EcoRII CCWGG 4 cut(s) 107, 228, 421, 912
EcoT38I GRGCYC 5 cut(s) 517, 767, 803, 941, 1013
Esp3I CGTCTC 1 cut(s) 200
FaeI CATG 2 cut(s) 586, 859
FaqI GGGAC 2 cut(s) 571, 1036
FatI CATG 2 cut(s) 582, 855
FauNDI CATATG 1 cut(s) 532
FblI GTMKAC 1 cut(s) 836
Fnu4HI GCNGC 4 cut(s) 58, 126, 341, 1043
FokI GGATG 5 cut(s) 27, 438, 589, 749, 1037
FriOI GRGCYC 5 cut(s) 517, 767, 803, 941, 1013
Fsp4HI GCNGC 4 cut(s) 58, 126, 341, 1043
FspBI CTAG 4 cut(s) 612, 804, 1014, 1074
GlaI GCGC 1 cut(s) 94
GluI GCNGC 4 cut(s) 58, 126, 341, 1043
GsuI CTGGAG 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 96
HapII CCGG 4 cut(s) 54, 144, 288, 589
HhaI GCGC 1 cut(s) 95
Hin1II CATG 2 cut(s) 586, 859
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HinfI GANTC 5 cut(s) 430, 470, 555, 773, 887
HpaII CCGG 4 cut(s) 54, 144, 288, 589
HphI GGTGA 3 cut(s) 199, 353, 744
Hpy166II GTNNAC 4 cut(s) 837, 982, 998, 1071
Hpy188I TCNGA 2 cut(s) 45, 153
Hpy188III TCNNGA 7 cut(s) 86, 474, 542, 596, 804, 875, 1014
Hpy8I GTNNAC 4 cut(s) 837, 982, 998, 1071
Hpy99I CGWCG 2 cut(s) 68, 191
HpyAV CCTTC 6 cut(s) 460, 566, 651, 753, 809, 825
HpyCH4III ACNGT 2 cut(s) 794, 923
HpyCH4V TGCA 1 cut(s) 701
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 418
HpyF3I CTNAG 1 cut(s) 628
Hsp92II CATG 2 cut(s) 586, 859
HspAI GCGC 1 cut(s) 93
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 6 cut(s) 45, 148, 538, 728, 974, 1081
LmnI GCTCC 7 cut(s) 122, 520, 762, 798, 936, 991, 1008
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 3 cut(s) 69, 327, 408
MaeI CTAG 4 cut(s) 612, 804, 1014, 1074
MaeIII GTNAC 1 cut(s) 283
MalI GATC 6 cut(s) 47, 150, 540, 730, 976, 1083
MboI GATC 6 cut(s) 45, 148, 538, 728, 974, 1081
MboII GAAGA 7 cut(s) 19, 175, 215, 627, 896, 929, 965
MflI RGATCY 2 cut(s) 728, 974
MhlI GDGCHC 5 cut(s) 517, 767, 803, 941, 1013
MluCI AATT 2 cut(s) 387, 956
MlyI GAGTC 2 cut(s) 782, 896
MroNI GCCGGC 1 cut(s) 53
MseI TTAA 2 cut(s) 255, 1090
MspI CCGG 4 cut(s) 54, 144, 288, 589
MspR9I CCNGG 5 cut(s) 109, 230, 423, 589, 914
Mva1269I GAATGC 1 cut(s) 1030
MvaI CCWGG 4 cut(s) 109, 230, 423, 914
MvnI CGCG 2 cut(s) 29, 298
MwoI GCNNNNNNNGC 2 cut(s) 66, 418
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 589
NdeI CATATG 1 cut(s) 532
NdeII GATC 6 cut(s) 45, 148, 538, 728, 974, 1081
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 2 cut(s) 586, 859
NlaIV GGNNCC 3 cut(s) 70, 292, 600
NspI RCATGY 2 cut(s) 586, 859
PaeI GCATGC 1 cut(s) 859
PciI ACATGT 1 cut(s) 582
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 93
PctI GAATGC 1 cut(s) 1030
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 430, 470, 555
PinAI ACCGGT 1 cut(s) 287
PkrI GCNGC 4 cut(s) 59, 127, 342, 1044
PleI GAGTC 2 cut(s) 781, 895
PpsI GAGTC 2 cut(s) 781, 895
PpuMI RGGWCCY 1 cut(s) 598
PscI ACATGT 1 cut(s) 582
Psp124BI GAGCTC 5 cut(s) 517, 767, 803, 941, 1013
Psp5II RGGWCCY 1 cut(s) 598
Psp6I CCWGG 4 cut(s) 107, 228, 421, 912
PspGI CCWGG 4 cut(s) 107, 228, 421, 912
PspN4I GGNNCC 3 cut(s) 70, 292, 600
PspPI GGNCC 3 cut(s) 598, 910, 1068
PspPPI RGGWCCY 1 cut(s) 598
PstI CTGCAG 1 cut(s) 703
PsuI RGATCY 2 cut(s) 728, 974
RsaI GTAC 3 cut(s) 649, 823, 925
RsaNI GTAC 3 cut(s) 648, 822, 924
SacI GAGCTC 5 cut(s) 517, 767, 803, 941, 1013
SaqAI TTAA 2 cut(s) 255, 1090
SatI GCNGC 4 cut(s) 58, 126, 341, 1043
Sau3AI GATC 6 cut(s) 45, 148, 538, 728, 974, 1081
Sau96I GGNCC 3 cut(s) 598, 910, 1068
SchI GAGTC 2 cut(s) 782, 896
ScrFI CCNGG 5 cut(s) 109, 230, 423, 589, 914
SduI GDGCHC 5 cut(s) 517, 767, 803, 941, 1013
SfaNI GCATC 3 cut(s) 69, 327, 408
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 3 cut(s) 598, 910, 1068
SpeI ACTAGT 1 cut(s) 1073
SphI GCATGC 1 cut(s) 859
Sse9I AATT 2 cut(s) 387, 956
SsiI CCGC 6 cut(s) 29, 123, 126, 175, 340, 1042
SspMI CTAG 4 cut(s) 612, 804, 1014, 1074
SstI GAGCTC 5 cut(s) 517, 767, 803, 941, 1013
StyD4I CCNGG 5 cut(s) 107, 228, 421, 587, 912
TaaI ACNGT 2 cut(s) 794, 923
TaqI TCGA 5 cut(s) 63, 103, 484, 511, 876
TaqII GACCGA 1 cut(s) 965
TasI AATT 2 cut(s) 387, 956
TauI GCSGC 3 cut(s) 128, 343, 1045
TfiI GAWTC 3 cut(s) 430, 470, 555
Tru1I TTAA 2 cut(s) 255, 1090
Tru9I TTAA 2 cut(s) 255, 1090
TscAI CASTG 1 cut(s) 24
TseI GCWGC 1 cut(s) 57
TspDTI ATGAA 3 cut(s) 336, 399, 710
TspGWI ACGGA 1 cut(s) 1001
TspRI CASTG 1 cut(s) 24
VpaK11BI GGWCC 3 cut(s) 598, 910, 1068
XapI RAATTY 1 cut(s) 956
XbaI TCTAGA 2 cut(s) 803, 1013
XceI RCATGY 2 cut(s) 586, 859
XmiI GTMKAC 1 cut(s) 836
XspI CTAG 4 cut(s) 612, 804, 1014, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.