MD04G1024000.v1.1

Ribosomal protein S1-like RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005054
Physical Location & Seq
Forward (+)
2756763 .. 2762690
5928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1024000.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGCCACTGCAAACAAGTGCACTGACTCTGGGTTCAGCCTCCTCTAAGCTCTTTACTACCGGCGATGCCATTGCCTTCTGCAACAATGGCAGTAAGTCTCGTCTGTTGAACCACATCAACTTCTCAAGGCATTCCCAAAAGTCTCACTGCAAGAGAAGATGTTCGGTTTACGCGTCGAGCAGAGTCTCCGACACCCAACATTCCGGACTGGTTCAGGACTCGGCTGCCTCTGCAGAGGCCCTCCAAGACGCTCGGAGATCTGCTGATTGGAAGGTAGCAAAGGCTTATAGTGACAGTGGATTGATCTATGAGGGCATGGTTGAAGGATATAATGGTGGAGGTTTGCTGATTAAGTTTCATTCCCTAGTGGGGTTTCTTCCATTTCCACAGTTGAGCCCATCACATTTTTGCAAAGAACGGGGAAAAGGCCTCCAAGAGATTGCAAAAGGTTTACCTGGTTCACTTATATCCATGAAGGTGATCCAAGCAGACGAAGAGAACAAGAAACTAATTTTCTCAGAAAAGGAAGCTGTCTGGTCAAAGTTTTCTGAGCAAATTAGTGTTGGGGATGTTTTTGAAGCTAGGGTGGGTTCTATGGAGGATTATGGTGCATTTGTTCACCTGCGATTTCCTGATGGTCTTTATCATCTGACTGGACTAGTACATGTCTCAGAGGTTTCATGGGATTTAGTTCAGGATATAAGAGACATTTTAACTGAGGGTGACGACGTAAGGGTCAAGATTATTAATATTGATAGAGAAAAGTCAAGAATTACACTTTCCATTAAACAATTGGAGGAAGATCCGCTTCTAGAAACATTGGACAAAGTAATACCACAGGATGGCTCTGTAGATCCTGATTCTTTGAGCACTAGTACTAGCACTAGCAATGATATAGAACCTCTTCCAGGGCTTGCATCCATACTTGAAGAGCTAGTACAGGAAGATGGTATAGATGATGTACGAATCAGTCGCCAGGGGTTTGAGAAAAGGGTGGTTTCACAGGACCTGCAGCTTTGGCTTTCGAATTCACCGGCCATTAATCGGAAATTTACTCTCCTTGCTCGTGCGGGAAGGCAGGTTCAGGAAATACAACTGACGACAACACTTGATCAGGAAGGTATCAAAAAGGCCCTGCAGCATGTACTGGAGCGTGTGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1

Protein Analysis

388

Amino Acids

42.78

Weight (kDa)

5.65

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 188 - 263 1.1e-16 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 630
AasI GACNNNNNNGTC 1 cut(s) 734
Acc36I ACCTGC 3 cut(s) 630, 1017, 1069
AccB7I CCANNNNNTGG 1 cut(s) 842
AccII CGCG 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 203
AciI CCGC 2 cut(s) 806, 1070
AclWI GGATC 3 cut(s) 475, 797, 848
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 2 cut(s) 1027, 1049
AfaI GTAC 5 cut(s) 663, 877, 939, 963, 1146
AfiI CCNNNNNNNGG 4 cut(s) 369, 842, 908, 1044
AflIII ACRYGT 2 cut(s) 171, 664
AgsI TTSAA 4 cut(s) 109, 323, 578, 929
AhlI ACTAGT 2 cut(s) 658, 872
AjnI CCWGG 3 cut(s) 454, 907, 975
AloI GAACNNNNNNTCC 2 cut(s) 1065, 1097
AluBI AGCT 5 cut(s) 49, 530, 581, 934, 1015
AluI AGCT 5 cut(s) 49, 530, 581, 934, 1015
Alw21I GWGCWC 2 cut(s) 22, 872
Alw26I GTCTC 5 cut(s) 102, 147, 190, 673, 699
Alw44I GTGCAC 1 cut(s) 18
AlwI GGATC 3 cut(s) 475, 797, 848
AlwNI CAGNNNCTG 1 cut(s) 1009
Aor13HI TCCGGA 1 cut(s) 203
AoxI GGCC 4 cut(s) 237, 427, 1035, 1131
ApaLI GTGCAC 1 cut(s) 18
ApeKI GCWGC 3 cut(s) 224, 1012, 1138
ApoI RAATTY 2 cut(s) 1027, 1049
AseI ATTAAT 2 cut(s) 747, 1041
Asp700I GAANNNNTTC 2 cut(s) 160, 903
AspS9I GGNCC 3 cut(s) 238, 1006, 1132
AsuHPI GGTGA 4 cut(s) 490, 611, 734, 1023
AsuII TTCGAA 1 cut(s) 1025
AvaII GGWCC 1 cut(s) 1006
BaeGI GKGCMC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 398
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
BauI CACGAG 1 cut(s) 1065
Bbv12I GWGCWC 2 cut(s) 22, 872
BbvI GCAGC 3 cut(s) 211, 1024, 1150
BccI CCATC 4 cut(s) 406, 629, 836, 941
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 3 cut(s) 456, 909, 977
BclI TGATCA 1 cut(s) 1111
BcoDI GTCTC 5 cut(s) 102, 147, 190, 673, 699
BcuI ACTAGT 2 cut(s) 658, 872
BfaI CTAG 8 cut(s) 365, 582, 659, 812, 873, 879, 885, 935
BfmI CTRYAG 4 cut(s) 231, 849, 1010, 1136
BfuAI ACCTGC 3 cut(s) 630, 1017, 1069
BglII AGATCT 1 cut(s) 257
BisI GCNGC 3 cut(s) 225, 1013, 1139
BlsI GCNGC 3 cut(s) 226, 1014, 1140
BmcAI AGTACT 1 cut(s) 877
Bme1390I CCNGG 3 cut(s) 456, 909, 977
Bme18I GGWCC 1 cut(s) 1006
BmgT120I GGNCC 3 cut(s) 238, 1006, 1132
BmrFI CCNGG 3 cut(s) 456, 909, 977
BmsI GCATC 2 cut(s) 55, 926
Bpu14I TTCGAA 1 cut(s) 1025
BpuEI CTTGAG 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 908, 976
BsaWI WCCGGW 1 cut(s) 203
Bsc4I CCNNNNNNNGG 4 cut(s) 369, 842, 908, 1044
Bse118I RCCGGY 2 cut(s) 59, 1033
Bse1I ACTGG 3 cut(s) 213, 658, 1152
Bse3DI GCAATG 2 cut(s) 69, 895
BseAI TCCGGA 1 cut(s) 203
BseBI CCWGG 3 cut(s) 456, 909, 977
BseDI CCNNGG 2 cut(s) 908, 976
BseGI GGATG 3 cut(s) 574, 847, 917
BseLI CCNNNNNNNGG 4 cut(s) 369, 842, 908, 1044
BseMI GCAATG 2 cut(s) 69, 895
BseMII CTCAG 4 cut(s) 531, 540, 684, 708
BseNI ACTGG 3 cut(s) 213, 658, 1152
BseRI GAGGAG 1 cut(s) 31
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 3 cut(s) 211, 1024, 1150
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 4 cut(s) 239, 429, 1037, 1133
BsiHKAI GWGCWC 2 cut(s) 22, 872
BsiSI CCGG 3 cut(s) 60, 204, 1034
BslI CCNNNNNNNGG 4 cut(s) 369, 842, 908, 1044
BsmAI GTCTC 5 cut(s) 102, 147, 190, 673, 699
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 4 cut(s) 239, 429, 1037, 1133
Bsp119I TTCGAA 1 cut(s) 1025
Bsp1286I GDGCHC 3 cut(s) 22, 398, 872
Bsp13I TCCGGA 1 cut(s) 203
Bsp143I GATC 6 cut(s) 257, 303, 480, 802, 853, 1111
BspACI CCGC 2 cut(s) 806, 1070
BspANI GGCC 4 cut(s) 239, 429, 1037, 1133
BspCNI CTCAG 4 cut(s) 530, 541, 683, 709
BspEI TCCGGA 1 cut(s) 203
BspFNI CGCG 1 cut(s) 173
BspMAI CTGCAG 3 cut(s) 235, 1014, 1140
BspMI ACCTGC 3 cut(s) 630, 1017, 1069
BspPI GGATC 3 cut(s) 475, 797, 848
BspQI GCTCTTC 1 cut(s) 924
BspT104I TTCGAA 1 cut(s) 1025
BsrDI GCAATG 2 cut(s) 69, 895
BsrFI RCCGGY 2 cut(s) 59, 1033
BsrI ACTGG 3 cut(s) 213, 658, 1152
BssAI RCCGGY 2 cut(s) 59, 1033
BssECI CCNNGG 2 cut(s) 908, 976
BssMI GATC 6 cut(s) 257, 303, 480, 802, 853, 1111
BssSI CACGAG 1 cut(s) 1065
Bst2BI CACGAG 1 cut(s) 1065
Bst2UI CCWGG 3 cut(s) 456, 909, 977
Bst4CI ACNGT 2 cut(s) 296, 390
Bst6I CTCTTC 3 cut(s) 489, 909, 924
BstBI TTCGAA 1 cut(s) 1025
BstC8I GCNNGC 1 cut(s) 915
BstDEI CTNAG 5 cut(s) 45, 517, 549, 670, 717
BstENI CCTNNNNNAGG 1 cut(s) 906
BstF5I GGATG 3 cut(s) 574, 847, 917
BstFNI CGCG 1 cut(s) 173
BstKTI GATC 6 cut(s) 260, 306, 483, 805, 856, 1114
BstMAI GTCTC 5 cut(s) 102, 147, 190, 673, 699
BstMBI GATC 6 cut(s) 257, 303, 480, 802, 853, 1111
BstMWI GCNNNNNNNGC 3 cut(s) 87, 230, 1018
BstNI CCWGG 3 cut(s) 456, 909, 977
BstNSI RCATGY 2 cut(s) 668, 1145
BstSCI CCNGG 3 cut(s) 454, 907, 975
BstSFI CTRYAG 4 cut(s) 231, 849, 1010, 1136
BstSLI GKGCMC 1 cut(s) 22
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 211, 1024, 1150
BstX2I RGATCY 3 cut(s) 257, 802, 853
BstYI RGATCY 3 cut(s) 257, 802, 853
BsuRI GGCC 4 cut(s) 239, 429, 1037, 1133
BtgZI GCGATG 1 cut(s) 78
BtsCI GGATG 3 cut(s) 574, 847, 917
BtsI GCAGTG 2 cut(s) 5, 145
BtsIMutI CAGTG 4 cut(s) 5, 20, 145, 301
BveI ACCTGC 3 cut(s) 630, 1017, 1069
Cac8I GCNNGC 1 cut(s) 915
CaiI CAGNNNCTG 1 cut(s) 1009
Cfr10I RCCGGY 2 cut(s) 59, 1033
Cfr13I GGNCC 3 cut(s) 238, 1006, 1132
CseI GACGC 2 cut(s) 162, 257
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 5 cut(s) 662, 876, 938, 962, 1145
CviAII CATG 5 cut(s) 316, 472, 665, 681, 1142
CviQI GTAC 5 cut(s) 662, 876, 938, 962, 1145
DdeI CTNAG 5 cut(s) 45, 517, 549, 670, 717
DpnI GATC 6 cut(s) 259, 305, 482, 804, 855, 1113
DpnII GATC 6 cut(s) 257, 303, 480, 802, 853, 1111
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 3 cut(s) 489, 909, 924
EarI CTCTTC 3 cut(s) 489, 909, 924
Eco147I AGGCCT 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 398
Eco47I GGWCC 1 cut(s) 1006
EcoNI CCTNNNNNAGG 1 cut(s) 906
EcoO109I RGGNCCY 3 cut(s) 238, 1006, 1132
EcoRI GAATTC 1 cut(s) 1027
EcoRII CCWGG 3 cut(s) 454, 907, 975
EcoT38I GRGCYC 1 cut(s) 398
FaeI CATG 5 cut(s) 319, 475, 668, 684, 1145
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FatI CATG 5 cut(s) 315, 471, 664, 680, 1141
FauI CCCGC 1 cut(s) 1063
FbaI TGATCA 1 cut(s) 1111
Fnu4HI GCNGC 3 cut(s) 225, 1013, 1139
FokI GGATG 3 cut(s) 581, 854, 904
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 3 cut(s) 225, 1013, 1139
FspBI CTAG 8 cut(s) 365, 582, 659, 812, 873, 879, 885, 935
GluI GCNGC 3 cut(s) 225, 1013, 1139
HaeIII GGCC 4 cut(s) 239, 429, 1037, 1133
HapII CCGG 3 cut(s) 60, 204, 1034
HgaI GACGC 2 cut(s) 162, 257
Hin1II CATG 5 cut(s) 319, 475, 668, 684, 1145
HinfI GANTC 5 cut(s) 25, 183, 218, 860, 966
HpaII CCGG 3 cut(s) 60, 204, 1034
HphI GGTGA 4 cut(s) 490, 611, 734, 1023
Hpy166II GTNNAC 5 cut(s) 20, 169, 452, 461, 619
Hpy188I TCNGA 7 cut(s) 190, 255, 520, 550, 651, 673, 1047
Hpy8I GTNNAC 5 cut(s) 20, 169, 452, 461, 619
Hpy99I CGWCG 2 cut(s) 178, 731
HpyAV CCTTC 6 cut(s) 85, 265, 317, 469, 1068, 1112
HpyCH4III ACNGT 2 cut(s) 296, 390
HpyCH4IV ACGT 1 cut(s) 729
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 230, 1018
HpyF3I CTNAG 5 cut(s) 45, 517, 549, 670, 717
HpySE526I ACGT 1 cut(s) 729
Hsp92II CATG 5 cut(s) 319, 475, 668, 684, 1145
Kpn2I TCCGGA 1 cut(s) 203
Ksp22I TGATCA 1 cut(s) 1111
Kzo9I GATC 6 cut(s) 257, 303, 480, 802, 853, 1111
LguI GCTCTTC 1 cut(s) 924
LmnI GCTCC 1 cut(s) 1150
Lsp1109I GCAGC 3 cut(s) 211, 1024, 1150
LweI GCATC 2 cut(s) 55, 926
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 8 cut(s) 365, 582, 659, 812, 873, 879, 885, 935
MaeII ACGT 1 cut(s) 729
MaeIII GTNAC 2 cut(s) 290, 722
MalI GATC 6 cut(s) 259, 305, 482, 804, 855, 1113
MboI GATC 6 cut(s) 257, 303, 480, 802, 853, 1111
MboII GAAGA 7 cut(s) 168, 368, 506, 812, 896, 941, 956
MfeI CAATTG 1 cut(s) 791
MflI RGATCY 3 cut(s) 257, 802, 853
MhlI GDGCHC 3 cut(s) 22, 398, 872
MluCI AATT 6 cut(s) 510, 555, 771, 791, 1027, 1049
MluI ACGCGT 1 cut(s) 171
MlyI GAGTC 3 cut(s) 19, 192, 212
MmeI TCCRAC 1 cut(s) 213
MroI TCCGGA 1 cut(s) 203
MroXI GAANNNNTTC 2 cut(s) 160, 903
MseI TTAA 5 cut(s) 351, 713, 747, 786, 1041
MslI CAYNNNNRTG 1 cut(s) 476
MspI CCGG 3 cut(s) 60, 204, 1034
MspR9I CCNGG 3 cut(s) 456, 909, 977
MunI CAATTG 1 cut(s) 791
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 3 cut(s) 456, 909, 977
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 87, 230, 1018
NdeII GATC 6 cut(s) 257, 303, 480, 802, 853, 1111
NlaIII CATG 5 cut(s) 319, 475, 668, 684, 1145
NmeAIII GCCGAG 1 cut(s) 200
NmuCI GTSAC 2 cut(s) 290, 722
NspI RCATGY 2 cut(s) 668, 1145
NspV TTCGAA 1 cut(s) 1025
PaqCI CACCTGC 1 cut(s) 630
PceI AGGCCT 1 cut(s) 429
PciI ACATGT 1 cut(s) 664
PciSI GCTCTTC 1 cut(s) 924
PcsI WCGNNNNNNNCGW 1 cut(s) 170
PctI GAATGC 1 cut(s) 130
PdmI GAANNNNTTC 2 cut(s) 160, 903
PfeI GAWTC 2 cut(s) 860, 966
PflMI CCANNNNNTGG 1 cut(s) 842
PkrI GCNGC 3 cut(s) 226, 1014, 1140
PleI GAGTC 3 cut(s) 19, 191, 212
PpsI GAGTC 3 cut(s) 19, 191, 212
PpuMI RGGWCCY 1 cut(s) 1006
PscI ACATGT 1 cut(s) 664
PshBI ATTAAT 2 cut(s) 747, 1041
Psp5II RGGWCCY 1 cut(s) 1006
Psp6I CCWGG 3 cut(s) 454, 907, 975
PspGI CCWGG 3 cut(s) 454, 907, 975
PspPI GGNCC 3 cut(s) 238, 1006, 1132
PspPPI RGGWCCY 1 cut(s) 1006
PstI CTGCAG 3 cut(s) 235, 1014, 1140
PstNI CAGNNNCTG 1 cut(s) 1009
PsuI RGATCY 3 cut(s) 257, 802, 853
RsaI GTAC 5 cut(s) 663, 877, 939, 963, 1146
RsaNI GTAC 5 cut(s) 662, 876, 938, 962, 1145
RseI CAYNNNNRTG 1 cut(s) 476
SapI GCTCTTC 1 cut(s) 924
SaqAI TTAA 5 cut(s) 351, 713, 747, 786, 1041
SatI GCNGC 3 cut(s) 225, 1013, 1139
Sau3AI GATC 6 cut(s) 257, 303, 480, 802, 853, 1111
Sau96I GGNCC 3 cut(s) 238, 1006, 1132
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 3 cut(s) 19, 192, 212
ScrFI CCNGG 3 cut(s) 456, 909, 977
SduI GDGCHC 3 cut(s) 22, 398, 872
SexAI ACCWGGT 1 cut(s) 454
SfaNI GCATC 2 cut(s) 55, 926
SfcI CTRYAG 4 cut(s) 231, 849, 1010, 1136
SfuI TTCGAA 1 cut(s) 1025
SinI GGWCC 1 cut(s) 1006
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
SpeI ACTAGT 2 cut(s) 658, 872
Sse9I AATT 6 cut(s) 510, 555, 771, 791, 1027, 1049
SseBI AGGCCT 1 cut(s) 429
SsiI CCGC 2 cut(s) 806, 1070
SspI AATATT 1 cut(s) 751
SspMI CTAG 8 cut(s) 365, 582, 659, 812, 873, 879, 885, 935
StuI AGGCCT 1 cut(s) 429
StyD4I CCNGG 3 cut(s) 454, 907, 975
TaaI ACNGT 2 cut(s) 296, 390
TaiI ACGT 1 cut(s) 732
TaqI TCGA 2 cut(s) 176, 1025
TasI AATT 6 cut(s) 510, 555, 771, 791, 1027, 1049
TatI WGTACW 4 cut(s) 661, 875, 937, 1144
TfiI GAWTC 2 cut(s) 860, 966
Tru1I TTAA 5 cut(s) 351, 713, 747, 786, 1041
Tru9I TTAA 5 cut(s) 351, 713, 747, 786, 1041
TscAI CASTG 4 cut(s) 12, 27, 152, 301
TseFI GTSAC 2 cut(s) 290, 722
TseI GCWGC 3 cut(s) 224, 1012, 1138
Tsp45I GTSAC 2 cut(s) 290, 722
TspDTI ATGAA 3 cut(s) 347, 488, 669
TspRI CASTG 4 cut(s) 12, 27, 152, 301
Van91I CCANNNNNTGG 1 cut(s) 842
VneI GTGCAC 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 1006
VspI ATTAAT 2 cut(s) 747, 1041
XagI CCTNNNNNAGG 1 cut(s) 906
XapI RAATTY 2 cut(s) 1027, 1049
XbaI TCTAGA 1 cut(s) 811
XceI RCATGY 2 cut(s) 668, 1145
XcmI CCANNNNNNNNNTGG 1 cut(s) 790
XmnI GAANNNNTTC 2 cut(s) 160, 903
XspI CTAG 8 cut(s) 365, 582, 659, 812, 873, 879, 885, 935
ZrmI AGTACT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.