Rw4G013970

Ribosomal protein S1-like RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005054
Physical Location & Seq
Reverse (-)
32669449 .. 32676367
6919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013970.1

Sequence Viewer

Length: 1236 bp
ATGGGCATATGGGCAGCGAGGGTTCTGTGGGCTAAGATAGGCCACTTGGAAGGTTCAAACACTGCGTTCATGTCTTCCACACCAAAAAATATGATACGTTATCCAATCCCCCATTTTGCAAAACCCACAATCCAAATGCTAATGCTGCTCGATGCCTCCTCCTCCTCCTCCTCTAATACTTGCTCAGTCTCTTCTTGTTTCTTAAACCACTCCAACTTCCCAAAGCAATCCCACAAGTCTGTAAGCAAGAAGAGAACCTCTCTAGTTTGCGCTGCCAGCAAGACTGAAGTGCAAGACTCCTCCCCTCTACTGAGCAGGCTCGGGTGTGTTCAAAGGTCTGCTGATTGGAAGGTAGCAAAAGCTTACAGTGATAGTGGATTCATCTTTGATGGGATGGTTGAAGGGTATAACAGTGGAGGTTTGCTGGTTCGGTTTCATTCTTTAGTGGGTTTTCTTCCATTTCCACAATTGAGCCCATCACACTTTTGCCAAGAACCGGGGAAAGGCGTCCAGGAGATTGCAAAAGGATTACTTGGTTCTCTTATATCTGTTAAGGTAATCCAAGCAGATGAAGAGAACAAGAAACTGATATTCTCAGAAAAGGAAGCTGTATGGTCCAAGTTTTCTGAGAAAATTAGTGTTGGGGATGTTTTCGAAGCTAGGGTTGGTTCTGTGGAGGATTATGGTGCTTTTGTTCATCTACTATTTCCTGATGGTCTTTATCATTTGACTGGGCTAGTACATGTCTCGGAGGTTTCATGGGATCTAGTTCAGGATGTAAGAGACATTTTGTCGGAGGGCGATGCAGTAAGAGTTAAGATCATTAATATTGATCGAGAAAAGTCAAGAATCACATTATCAATTAAACAGTTGGAGGAAGATCCACTTTTAGAAACATTGGACAAAGTGATACCCCAGGATGGCTCTGACGGTCCTGATTCTTTGAACCCTAACAATAGCAATAATATAGAACCTCTTCCAGGCCTTGAAGCCATATTCGAAGAGCTACTACAGGAACAGGGAATAGATGATGTAGGAATCACTCGCCAAGGATTTGAGAAAAGGGTGGTTTCACAGGATCTGCAGCTTTGGCTTTCTAATTCACCAGCCATTAACCAGAAGTTTACTCTCCTTGCTCGGGCAGGAAGGCAGGTACAGGAAATACAACTGACGACAACACTTGATCAGGAAGGTATAAAACAAGCACTCCAGCGAGTATTGGAGCGTGTCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
S1

Protein Analysis

411

Amino Acids

45.41

Weight (kDa)

5.68

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 213 - 289 1.6e-17 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1141
AclWI GGATC 3 cut(s) 771, 875, 1086
AcuI CTGAAG 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 2 cut(s) 741, 1155
AfiI CCNNNNNNNGG 5 cut(s) 496, 503, 920, 980, 1138
AflIII ACRYGT 1 cut(s) 742
AgsI TTSAA 5 cut(s) 57, 332, 401, 946, 989
AhdI GACNNNNNGTC 1 cut(s) 790
AjnI CCWGG 3 cut(s) 510, 915, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AluBI AGCT 5 cut(s) 362, 608, 659, 1006, 1087
AluI AGCT 5 cut(s) 362, 608, 659, 1006, 1087
Alw26I GTCTC 3 cut(s) 193, 751, 777
AlwI GGATC 3 cut(s) 771, 875, 1086
AlwNI CAGNNNCTG 1 cut(s) 1081
Ama87I CYCGRG 2 cut(s) 320, 1137
AoxI GGCC 2 cut(s) 40, 982
ApeKI GCWGC 4 cut(s) 14, 145, 272, 1084
AseI ATTAAT 1 cut(s) 825
Asp700I GAANNNNTTC 1 cut(s) 975
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 2 cut(s) 615, 932
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 1 cut(s) 1095
AsuII TTCGAA 2 cut(s) 654, 999
AvaI CYCGRG 2 cut(s) 320, 1137
AvaII GGWCC 2 cut(s) 615, 932
BanII GRGCYC 1 cut(s) 476
BarI GAAGNNNNNNTAC 2 cut(s) 993, 1025
BbsI GAAGAC 1 cut(s) 66
BbvI GCAGC 4 cut(s) 26, 132, 259, 1096
BccI CCATC 5 cut(s) 383, 388, 484, 707, 914
BciT130I CCWGG 3 cut(s) 512, 917, 981
BclI TGATCA 1 cut(s) 1183
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 3 cut(s) 193, 751, 777
BfaI CTAG 4 cut(s) 263, 660, 737, 767
BfmI CTRYAG 2 cut(s) 1010, 1082
BfuAI ACCTGC 1 cut(s) 1141
BisI GCNGC 4 cut(s) 15, 146, 273, 1085
BlsI GCNGC 4 cut(s) 16, 147, 274, 1086
Bme1390I CCNGG 4 cut(s) 498, 512, 917, 981
Bme18I GGWCC 2 cut(s) 615, 932
BmeRI GACNNNNNGTC 1 cut(s) 790
BmeT110I CYCGRG 2 cut(s) 320, 1137
BmgT120I GGNCC 2 cut(s) 615, 932
BmrFI CCNGG 4 cut(s) 498, 512, 917, 981
BmrI ACTGGG 1 cut(s) 741
BmsI GCATC 2 cut(s) 142, 793
BmuI ACTGGG 1 cut(s) 741
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 244, 276
BpmI CTGGAG 1 cut(s) 1193
Bpu14I TTCGAA 2 cut(s) 654, 999
BpuMI CCSGG 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 3 cut(s) 497, 915, 1048
BsaXI ACNNNNNCTCC 2 cut(s) 1191, 1221
Bsc4I CCNNNNNNNGG 5 cut(s) 496, 503, 920, 980, 1138
Bse1I ACTGG 1 cut(s) 736
BseBI CCWGG 3 cut(s) 512, 917, 981
BseDI CCNNGG 3 cut(s) 497, 915, 1048
BseGI GGATG 4 cut(s) 399, 652, 781, 925
BseLI CCNNNNNNNGG 5 cut(s) 496, 503, 920, 980, 1138
BseMII CTCAG 4 cut(s) 198, 302, 609, 618
BseNI ACTGG 1 cut(s) 736
BseRI GAGGAG 6 cut(s) 148, 151, 154, 157, 160, 289
BseXI GCAGC 4 cut(s) 26, 132, 259, 1096
BshFI GGCC 2 cut(s) 42, 984
BsiHKCI CYCGRG 2 cut(s) 320, 1137
BsiSI CCGG 1 cut(s) 497
BslFI GGGAC 1 cut(s) 1214
BslI CCNNNNNNNGG 5 cut(s) 496, 503, 920, 980, 1138
BsmAI GTCTC 3 cut(s) 193, 751, 777
BsmFI GGGAC 1 cut(s) 1214
BsnI GGCC 2 cut(s) 42, 984
BsoBI CYCGRG 2 cut(s) 320, 1137
Bsp119I TTCGAA 2 cut(s) 654, 999
Bsp1286I GDGCHC 1 cut(s) 476
Bsp143I GATC 6 cut(s) 763, 819, 832, 880, 1078, 1183
BspANI GGCC 2 cut(s) 42, 984
BspCNI CTCAG 4 cut(s) 197, 303, 608, 619
BspMAI CTGCAG 1 cut(s) 1086
BspMI ACCTGC 1 cut(s) 1141
BspPI GGATC 3 cut(s) 771, 875, 1086
BspQI GCTCTTC 1 cut(s) 996
BspT104I TTCGAA 2 cut(s) 654, 999
BsrI ACTGG 1 cut(s) 736
BssECI CCNNGG 3 cut(s) 497, 915, 1048
BssMI GATC 6 cut(s) 763, 819, 832, 880, 1078, 1183
BssNI GRCGYC 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 1048
Bst2UI CCWGG 3 cut(s) 512, 917, 981
Bst4CI ACNGT 4 cut(s) 368, 413, 870, 932
Bst6I CTCTTC 5 cut(s) 196, 245, 567, 981, 996
BstACI GRCGYC 1 cut(s) 507
BstBI TTCGAA 2 cut(s) 654, 999
BstC8I GCNNGC 2 cut(s) 277, 317
BstDEI CTNAG 5 cut(s) 33, 184, 311, 595, 627
BstENI CCTNNNNNAGG 1 cut(s) 978
BstF5I GGATG 4 cut(s) 399, 652, 781, 925
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 6 cut(s) 766, 822, 835, 883, 1081, 1186
BstMAI GTCTC 3 cut(s) 193, 751, 777
BstMBI GATC 6 cut(s) 763, 819, 832, 880, 1078, 1183
BstMWI GCNNNNNNNGC 3 cut(s) 145, 276, 1090
BstNI CCWGG 3 cut(s) 512, 917, 981
BstNSI RCATGY 1 cut(s) 746
BstSCI CCNGG 4 cut(s) 496, 510, 915, 979
BstSFI CTRYAG 2 cut(s) 1010, 1082
BstV1I GCAGC 4 cut(s) 26, 132, 259, 1096
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 3 cut(s) 763, 880, 1078
BstYI RGATCY 3 cut(s) 763, 880, 1078
BsuRI GGCC 2 cut(s) 42, 984
BtgZI GCGATG 1 cut(s) 816
BtsCI GGATG 4 cut(s) 399, 652, 781, 925
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 3 cut(s) 60, 373, 418
BveI ACCTGC 1 cut(s) 1141
Cac8I GCNNGC 2 cut(s) 277, 317
CaiI CAGNNNCTG 1 cut(s) 1081
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 615, 932
CseI GACGC 1 cut(s) 496
Csp6I GTAC 2 cut(s) 740, 1154
CviAII CATG 3 cut(s) 70, 743, 759
CviQI GTAC 2 cut(s) 740, 1154
DdeI CTNAG 5 cut(s) 33, 184, 311, 595, 627
DpnI GATC 6 cut(s) 765, 821, 834, 882, 1080, 1185
DpnII GATC 6 cut(s) 763, 819, 832, 880, 1078, 1183
DriI GACNNNNNGTC 1 cut(s) 790
Eam1104I CTCTTC 5 cut(s) 196, 245, 567, 981, 996
Eam1105I GACNNNNNGTC 1 cut(s) 790
EarI CTCTTC 5 cut(s) 196, 245, 567, 981, 996
Eco130I CCWWGG 1 cut(s) 1048
Eco147I AGGCCT 1 cut(s) 984
Eco24I GRGCYC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 615, 932
Eco57I CTGAAG 1 cut(s) 306
Eco88I CYCGRG 2 cut(s) 320, 1137
EcoNI CCTNNNNNAGG 1 cut(s) 978
EcoRII CCWGG 3 cut(s) 510, 915, 979
EcoT14I CCWWGG 1 cut(s) 1048
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 1048
FaeI CATG 3 cut(s) 73, 746, 762
FalI AAGNNNNNCTT 4 cut(s) 516, 548, 870, 902
FaqI GGGAC 1 cut(s) 1214
FatI CATG 3 cut(s) 69, 742, 758
FauNDI CATATG 1 cut(s) 8
FbaI TGATCA 1 cut(s) 1183
Fnu4HI GCNGC 4 cut(s) 15, 146, 273, 1085
FokI GGATG 4 cut(s) 406, 659, 788, 932
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 4 cut(s) 15, 146, 273, 1085
FspBI CTAG 4 cut(s) 263, 660, 737, 767
GlaI GCGC 1 cut(s) 271
GluI GCNGC 4 cut(s) 15, 146, 273, 1085
GsuI CTGGAG 1 cut(s) 1193
HaeIII GGCC 2 cut(s) 42, 984
HapII CCGG 1 cut(s) 497
HgaI GACGC 1 cut(s) 496
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 3 cut(s) 73, 746, 762
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 5 cut(s) 296, 378, 849, 938, 1038
HpaII CCGG 1 cut(s) 497
HphI GGTGA 1 cut(s) 1095
Hpy166II GTNNAC 1 cut(s) 1125
Hpy188I TCNGA 5 cut(s) 598, 628, 751, 796, 928
Hpy188III TCNNGA 6 cut(s) 710, 773, 836, 846, 935, 1187
Hpy8I GTNNAC 1 cut(s) 1125
HpyAV CCTTC 5 cut(s) 44, 343, 395, 1140, 1184
HpyCH4III ACNGT 4 cut(s) 368, 413, 870, 932
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 5 cut(s) 119, 292, 521, 806, 1084
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 276, 1090
HpyF3I CTNAG 5 cut(s) 33, 184, 311, 595, 627
HpySE526I ACGT 1 cut(s) 97
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 3 cut(s) 73, 746, 762
HspAI GCGC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 1183
Kzo9I GATC 6 cut(s) 763, 819, 832, 880, 1078, 1183
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 1 cut(s) 1222
Lsp1109I GCAGC 4 cut(s) 26, 132, 259, 1096
LweI GCATC 2 cut(s) 142, 793
MaeI CTAG 4 cut(s) 263, 660, 737, 767
MaeII ACGT 1 cut(s) 97
MalI GATC 6 cut(s) 765, 821, 834, 882, 1080, 1185
MboI GATC 6 cut(s) 763, 819, 832, 880, 1078, 1183
MboII GAAGA 8 cut(s) 66, 183, 262, 446, 584, 890, 968, 1013
MfeI CAATTG 1 cut(s) 467
MflI RGATCY 3 cut(s) 763, 880, 1078
MhlI GDGCHC 1 cut(s) 476
MluCI AATT 4 cut(s) 467, 633, 861, 1099
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 3 cut(s) 237, 774, 852
MroXI GAANNNNTTC 1 cut(s) 975
MseI TTAA 6 cut(s) 203, 552, 816, 825, 864, 1113
MspI CCGG 1 cut(s) 497
MspR9I CCNGG 4 cut(s) 498, 512, 917, 981
MunI CAATTG 1 cut(s) 467
MvaI CCWGG 3 cut(s) 512, 917, 981
MwoI GCNNNNNNNGC 3 cut(s) 145, 276, 1090
NciI CCSGG 1 cut(s) 498
NdeI CATATG 1 cut(s) 8
NdeII GATC 6 cut(s) 763, 819, 832, 880, 1078, 1183
NlaIII CATG 3 cut(s) 73, 746, 762
NspI RCATGY 1 cut(s) 746
NspV TTCGAA 2 cut(s) 654, 999
PceI AGGCCT 1 cut(s) 984
PciI ACATGT 1 cut(s) 742
PciSI GCTCTTC 1 cut(s) 996
PdmI GAANNNNTTC 1 cut(s) 975
PfeI GAWTC 4 cut(s) 378, 849, 938, 1038
PfoI TCCNGGA 1 cut(s) 510
PkrI GCNGC 4 cut(s) 16, 147, 274, 1086
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
PscI ACATGT 1 cut(s) 742
PshBI ATTAAT 1 cut(s) 825
Psp6I CCWGG 3 cut(s) 510, 915, 979
PspGI CCWGG 3 cut(s) 510, 915, 979
PspPI GGNCC 2 cut(s) 615, 932
PstI CTGCAG 1 cut(s) 1086
PstNI CAGNNNCTG 1 cut(s) 1081
PsuI RGATCY 3 cut(s) 763, 880, 1078
RsaI GTAC 2 cut(s) 741, 1155
RsaNI GTAC 2 cut(s) 740, 1154
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 6 cut(s) 203, 552, 816, 825, 864, 1113
SatI GCNGC 4 cut(s) 15, 146, 273, 1085
Sau3AI GATC 6 cut(s) 763, 819, 832, 880, 1078, 1183
Sau96I GGNCC 2 cut(s) 615, 932
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 4 cut(s) 498, 512, 917, 981
SduI GDGCHC 1 cut(s) 476
SfaNI GCATC 2 cut(s) 142, 793
SfcI CTRYAG 2 cut(s) 1010, 1082
SfuI TTCGAA 2 cut(s) 654, 999
SinI GGWCC 2 cut(s) 615, 932
Sse9I AATT 4 cut(s) 467, 633, 861, 1099
SseBI AGGCCT 1 cut(s) 984
SspI AATATT 1 cut(s) 829
SspMI CTAG 4 cut(s) 263, 660, 737, 767
StuI AGGCCT 1 cut(s) 984
StyD4I CCNGG 4 cut(s) 496, 510, 915, 979
StyI CCWWGG 1 cut(s) 1048
TaaI ACNGT 4 cut(s) 368, 413, 870, 932
TaiI ACGT 1 cut(s) 100
TaqI TCGA 4 cut(s) 150, 654, 835, 999
TasI AATT 4 cut(s) 467, 633, 861, 1099
TatI WGTACW 1 cut(s) 739
TfiI GAWTC 4 cut(s) 378, 849, 938, 1038
Tru1I TTAA 6 cut(s) 203, 552, 816, 825, 864, 1113
Tru9I TTAA 6 cut(s) 203, 552, 816, 825, 864, 1113
TscAI CASTG 3 cut(s) 67, 373, 418
TseI GCWGC 4 cut(s) 14, 145, 272, 1084
TspDTI ATGAA 6 cut(s) 58, 370, 425, 585, 686, 747
TspRI CASTG 3 cut(s) 67, 373, 418
VpaK11BI GGWCC 2 cut(s) 615, 932
VspI ATTAAT 1 cut(s) 825
XagI CCTNNNNNAGG 1 cut(s) 978
XceI RCATGY 1 cut(s) 746
XmnI GAANNNNTTC 1 cut(s) 975
XspI CTAG 4 cut(s) 263, 660, 737, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.