MD04G1042000.v1.1

FAR1 DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004298
Physical Location & Seq
Reverse (-)
4969420 .. 4973244
3825 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1042000.v1.1.491

Sequence Viewer

Length: 501 bp
ATGCTTTTTGAATCAGAAGAAGCTGCCAAAGTATTCTATGATGATTATGCCAGGCGTGTTGGATTTGTGACCCGTGTTTTGTCATCCCGGAAGTCTGAGCGTGATGGGTCAATAATCTCTCGTGGACTTGGATGCAGAGGGGTCTCTGAGAATCGTAGAAAAGTAATGCAGAAGGAGTGTGGAGAAGGAGAAGTTTGTCCAGCCATGGTCTTGTTGAAACAAGAGATGCCTGGAAGATGGGTTATCAAGAAATTTTTGGGGGACCATAATCATCTGTTAGTGATTCAATCACCAAAGATCCGCAGAACGCTTGATGAGAAAGATAAGAAAATCCAGGAGTTATCTGCAGAGCTACGAGTTAAGAAGCGGTTAAGTGCAGCGTATAGAGATCAGCTACTTGCCTTTATCAAGGATATTGAAGACCACAACGCGCACCTTTCAACAAAAGTTCAATCGGTTTCTAACAAGCTAAAAGAGCTTGAAGCTAAAACAGTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

167

Amino Acids

19.03

Weight (kDa)

9.56

Isoelectric Point (pI)

57.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 12 - 94 9.2e-17 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 431
AciI CCGC 2 cut(s) 301, 367
AclWI GGATC 1 cut(s) 292
AcsI RAATTY 1 cut(s) 251
AgsI TTSAA 7 cut(s) 11, 217, 287, 419, 441, 452, 482
AjnI CCWGG 3 cut(s) 50, 229, 333
AluBI AGCT 6 cut(s) 23, 352, 394, 469, 478, 485
AluI AGCT 6 cut(s) 23, 352, 394, 469, 478, 485
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 1 cut(s) 292
ApeKI GCWGC 2 cut(s) 23, 377
ApoI RAATTY 1 cut(s) 251
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 282
AvaII GGWCC 1 cut(s) 262
BauI CACGAG 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 426
BbvI GCAGC 2 cut(s) 10, 389
BccI CCATC 2 cut(s) 98, 231
BciT130I CCWGG 3 cut(s) 52, 231, 335
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 148
BfmI CTRYAG 1 cut(s) 345
BisI GCNGC 2 cut(s) 24, 378
BlsI GCNGC 2 cut(s) 25, 379
Bme1390I CCNGG 4 cut(s) 52, 88, 231, 335
Bme18I GGWCC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 4 cut(s) 52, 88, 231, 335
BmsI GCATC 2 cut(s) 122, 216
BpiI GAAGAC 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 88
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 204
BseBI CCWGG 3 cut(s) 52, 231, 335
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 2 cut(s) 83, 137
BseMII CTCAG 2 cut(s) 87, 138
BseXI GCAGC 2 cut(s) 10, 389
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 431
BsiSI CCGG 1 cut(s) 88
BslFI GGGAC 1 cut(s) 275
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 275
Bso31I GGTCTC 1 cut(s) 148
Bsp143I GATC 2 cut(s) 297, 388
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 2 cut(s) 301, 367
BspCNI CTCAG 2 cut(s) 88, 139
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 1 cut(s) 263
BspMAI CTGCAG 1 cut(s) 349
BspPI GGATC 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 148
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 2 cut(s) 297, 388
BssSI CACGAG 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 120
Bst2UI CCWGG 3 cut(s) 52, 231, 335
Bst4CI ACNGT 1 cut(s) 493
BstDEI CTNAG 2 cut(s) 96, 147
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 2 cut(s) 83, 137
BstFNI CGCG 1 cut(s) 431
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 2 cut(s) 300, 391
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 2 cut(s) 297, 388
BstMWI GCNNNNNNNGC 1 cut(s) 475
BstNI CCWGG 3 cut(s) 52, 231, 335
BstSCI CCNGG 4 cut(s) 50, 86, 229, 333
BstSFI CTRYAG 1 cut(s) 345
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 2 cut(s) 10, 389
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 1 cut(s) 297
BstYI RGATCY 1 cut(s) 297
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 2 cut(s) 83, 137
CfoI GCGC 1 cut(s) 433
Cfr13I GGNCC 1 cut(s) 262
CviAII CATG 1 cut(s) 205
CviJI RGCY 7 cut(s) 23, 203, 352, 394, 469, 478, 485
CviKI_1 RGCY 7 cut(s) 23, 203, 352, 394, 469, 478, 485
DdeI CTNAG 2 cut(s) 96, 147
DpnI GATC 2 cut(s) 299, 390
DpnII GATC 2 cut(s) 297, 388
Eco130I CCWWGG 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 262
EcoRII CCWGG 3 cut(s) 50, 229, 333
EcoT14I CCWWGG 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 1 cut(s) 208
FaiI YATR 5 cut(s) 39, 48, 206, 267, 384
FaqI GGGAC 1 cut(s) 275
FatI CATG 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 24, 378
FokI GGATG 2 cut(s) 70, 144
Fsp4HI GCNGC 2 cut(s) 24, 378
GlaI GCGC 1 cut(s) 432
GluI GCNGC 2 cut(s) 24, 378
HapII CCGG 1 cut(s) 88
HhaI GCGC 1 cut(s) 433
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HinfI GANTC 3 cut(s) 11, 151, 283
HpaII CCGG 1 cut(s) 88
HphI GGTGA 1 cut(s) 282
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 3 cut(s) 16, 97, 148
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 1 cut(s) 125
HpyAV CCTTC 2 cut(s) 166, 179
HpyCH4III ACNGT 1 cut(s) 493
HpyCH4V TGCA 4 cut(s) 135, 169, 347, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 475
HpyF3I CTNAG 2 cut(s) 96, 147
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 1 cut(s) 431
Kzo9I GATC 2 cut(s) 297, 388
LpnPI CCDG 8 cut(s) 37, 64, 101, 213, 216, 243, 320, 347
Lsp1109I GCAGC 2 cut(s) 10, 389
LweI GCATC 2 cut(s) 122, 216
MaeIII GTNAC 1 cut(s) 67
MalI GATC 2 cut(s) 299, 390
MboI GATC 2 cut(s) 297, 388
MboII GAAGA 3 cut(s) 29, 246, 431
MflI RGATCY 1 cut(s) 297
MluCI AATT 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 40
MnlI CCTC 1 cut(s) 131
MseI TTAA 2 cut(s) 360, 371
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 4 cut(s) 52, 88, 231, 335
MvaI CCWGG 3 cut(s) 52, 231, 335
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 1 cut(s) 475
NciI CCSGG 1 cut(s) 88
NcoI CCATGG 1 cut(s) 204
NdeII GATC 2 cut(s) 297, 388
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 1 cut(s) 263
NmuCI GTSAC 1 cut(s) 67
PfeI GAWTC 3 cut(s) 11, 151, 283
PfoI TCCNGGA 2 cut(s) 86, 333
PkrI GCNGC 2 cut(s) 25, 379
Psp6I CCWGG 3 cut(s) 50, 229, 333
PspGI CCWGG 3 cut(s) 50, 229, 333
PspN4I GGNNCC 1 cut(s) 263
PspPI GGNCC 1 cut(s) 262
PstI CTGCAG 1 cut(s) 349
PsuI RGATCY 1 cut(s) 297
SaqAI TTAA 2 cut(s) 360, 371
SatI GCNGC 2 cut(s) 24, 378
Sau3AI GATC 2 cut(s) 297, 388
Sau96I GGNCC 1 cut(s) 262
ScrFI CCNGG 4 cut(s) 52, 88, 231, 335
SetI ASST 7 cut(s) 25, 354, 396, 438, 471, 480, 487
SfaNI GCATC 2 cut(s) 122, 216
SfcI CTRYAG 1 cut(s) 345
SinI GGWCC 1 cut(s) 262
Sse9I AATT 1 cut(s) 251
SsiI CCGC 2 cut(s) 301, 367
StyD4I CCNGG 4 cut(s) 50, 86, 229, 333
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 493
TasI AATT 1 cut(s) 251
TfiI GAWTC 3 cut(s) 11, 151, 283
Tru1I TTAA 2 cut(s) 360, 371
Tru9I TTAA 2 cut(s) 360, 371
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 23, 377
Tsp45I GTSAC 1 cut(s) 67
VpaK11BI GGWCC 1 cut(s) 262
XapI RAATTY 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.