MD04G1042100.v1.1

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004298
Physical Location & Seq
Reverse (-)
4974048 .. 4984779
10732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1042100.v1.1.491

Sequence Viewer

Length: 570 bp
ATGGAATTCGAATCTGAAGATGCTGCCAAGATATTTTATGATGAGTATGCACGGCATGTTGGATTTGTCATGCGTGTGATGTCATGTCGTCGGTCTGAAAGAGATGGTAGGATTCTTGCTCGTCGACTTGGGTGTAATAAGGAGGGACACTGTGTTAGTATTCGAGGTAAATTTGGGCCGGTTCGAAAACCACGAGCAAGTACAAGGGAAGGTTGCAAGGCAATGATTCACGTAAAGTATGATAAATCCAGTAAATGGGTGATTACAAAATTCATAAAGGATCATAATCATCCACTAGTGGTGTCCCCACGTGAAGCTCGTCAAACCTTGGATGAGAAGGATAAGAAAATTCAAGAATTAACTGCAGAGCTTCGGAACAAGAAACGGCTGTGCACTACATATCAGGAACAGCTAACGGCATTTACTGAGATCGTTGAAGAGTATAATGAACAGCTATCCAAGAAAGTTCAAAATTGCAGGCTGCAGCCATGCGAGCAAATCCAATTTGTCCGTGACTGCGCAGACGTGCAAGCACTGATGGAGCTGCTTAAAACGGTGACCAGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1

Protein Analysis

190

Amino Acids

22.06

Weight (kDa)

9.19

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 12 - 100 1.5e-27 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 520
AccB7I CCANNNNNTGG 1 cut(s) 255
AccI GTMKAC 1 cut(s) 124
AclWI GGATC 1 cut(s) 288
AcsI RAATTY 4 cut(s) 5, 170, 269, 348
AcuI CTGAAG 1 cut(s) 36
AcvI CACGTG 1 cut(s) 311
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 1 cut(s) 255
AgsI TTSAA 3 cut(s) 353, 437, 470
AhlI ACTAGT 1 cut(s) 295
AjiI CACGTC 1 cut(s) 526
AluBI AGCT 5 cut(s) 317, 370, 412, 454, 544
AluI AGCT 5 cut(s) 317, 370, 412, 454, 544
Alw21I GWGCWC 1 cut(s) 395
Alw44I GTGCAC 1 cut(s) 391
AlwI GGATC 1 cut(s) 288
AoxI GGCC 1 cut(s) 176
ApaLI GTGCAC 1 cut(s) 391
ApeKI GCWGC 4 cut(s) 23, 481, 484, 544
ApoI RAATTY 4 cut(s) 5, 170, 269, 348
AspLEI GCGC 1 cut(s) 521
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 2 cut(s) 271, 568
AsuII TTCGAA 2 cut(s) 9, 184
BaeGI GKGCMC 1 cut(s) 395
BauI CACGAG 1 cut(s) 192
BbrPI CACGTG 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 4 cut(s) 10, 468, 496, 531
BccI CCATC 2 cut(s) 98, 532
BceAI ACGGC 3 cut(s) 68, 401, 432
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 2 cut(s) 363, 482
BisI GCNGC 4 cut(s) 24, 482, 485, 545
BlsI GCNGC 4 cut(s) 25, 483, 486, 546
BmgBI CACGTC 1 cut(s) 526
BmgT120I GGNCC 1 cut(s) 176
BmsI GCATC 1 cut(s) 10
Bpu14I TTCGAA 2 cut(s) 9, 184
BsaAI YACGTR 2 cut(s) 232, 311
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 1 cut(s) 327
Bsc4I CCNNNNNNNGG 1 cut(s) 255
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 249, 561
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 2 cut(s) 289, 337
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 1 cut(s) 255
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 2 cut(s) 249, 561
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 4 cut(s) 10, 468, 496, 531
BshFI GGCC 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 395
BsiSI CCGG 1 cut(s) 179
BslFI GGGAC 2 cut(s) 159, 289
BslI CCNNNNNNNGG 1 cut(s) 255
BsmFI GGGAC 2 cut(s) 159, 289
BsnI GGCC 1 cut(s) 178
Bsp119I TTCGAA 2 cut(s) 9, 184
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 2 cut(s) 280, 429
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 1 cut(s) 418
BspHI TCATGA 1 cut(s) 566
BspMAI CTGCAG 2 cut(s) 367, 486
BspPI GGATC 1 cut(s) 288
BspT104I TTCGAA 2 cut(s) 9, 184
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 2 cut(s) 249, 561
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 2 cut(s) 280, 429
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 152, 556
Bst6I CTCTTC 1 cut(s) 432
BstBAI YACGTR 2 cut(s) 232, 311
BstBI TTCGAA 2 cut(s) 9, 184
BstC8I GCNNGC 3 cut(s) 479, 494, 531
BstDEI CTNAG 1 cut(s) 426
BstEII GGTNACC 1 cut(s) 556
BstF5I GGATG 2 cut(s) 289, 337
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 2 cut(s) 283, 432
BstMBI GATC 2 cut(s) 280, 429
BstMWI GCNNNNNNNGC 1 cut(s) 493
BstNSI RCATGY 1 cut(s) 59
BstPI GGTNACC 1 cut(s) 556
BstSFI CTRYAG 2 cut(s) 363, 482
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 4 cut(s) 10, 468, 496, 531
BsuRI GGCC 1 cut(s) 178
BtrI CACGTC 1 cut(s) 526
BtsCI GGATG 2 cut(s) 289, 337
BtsIMutI CAGTG 2 cut(s) 148, 533
Cac8I GCNNGC 3 cut(s) 479, 494, 531
CciI TCATGA 1 cut(s) 566
CfoI GCGC 1 cut(s) 521
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 201
CviAII CATG 5 cut(s) 56, 70, 84, 489, 567
CviJI RGCY 9 cut(s) 178, 317, 370, 388, 412, 454, 481, 487, 544
CviKI_1 RGCY 9 cut(s) 178, 317, 370, 388, 412, 454, 481, 487, 544
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 1 cut(s) 426
DpnI GATC 2 cut(s) 282, 431
DpnII GATC 2 cut(s) 280, 429
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 327
Eco57I CTGAAG 1 cut(s) 36
Eco72I CACGTG 1 cut(s) 311
Eco91I GGTNACC 1 cut(s) 556
EcoO65I GGTNACC 1 cut(s) 556
EcoRI GAATTC 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 5 cut(s) 59, 73, 87, 492, 570
FaqI GGGAC 2 cut(s) 159, 289
FatI CATG 5 cut(s) 55, 69, 83, 488, 566
FblI GTMKAC 1 cut(s) 124
Fnu4HI GCNGC 4 cut(s) 24, 482, 485, 545
FokI GGATG 2 cut(s) 276, 344
Fsp4HI GCNGC 4 cut(s) 24, 482, 485, 545
FspBI CTAG 1 cut(s) 296
FspI TGCGCA 1 cut(s) 520
GlaI GCGC 1 cut(s) 520
GluI GCNGC 4 cut(s) 24, 482, 485, 545
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 179
HhaI GCGC 1 cut(s) 521
Hin1II CATG 5 cut(s) 59, 73, 87, 492, 570
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HincII GTYRAC 1 cut(s) 125
HindII GTYRAC 1 cut(s) 125
HinfI GANTC 3 cut(s) 11, 112, 226
HpaII CCGG 1 cut(s) 179
HphI GGTGA 2 cut(s) 271, 568
Hpy166II GTNNAC 2 cut(s) 125, 393
Hpy188I TCNGA 3 cut(s) 16, 97, 375
Hpy188III TCNNGA 3 cut(s) 353, 404, 567
Hpy8I GTNNAC 2 cut(s) 125, 393
Hpy99I CGWCG 2 cut(s) 93, 126
HpyAV CCTTC 2 cut(s) 203, 331
HpyCH4III ACNGT 2 cut(s) 152, 556
HpyCH4IV ACGT 3 cut(s) 231, 310, 525
HpyCH4V TGCA 7 cut(s) 50, 216, 365, 393, 477, 484, 529
HpyF10VI GCNNNNNNNGC 1 cut(s) 493
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 3 cut(s) 231, 310, 525
Hsp92II CATG 5 cut(s) 59, 73, 87, 492, 570
HspAI GCGC 1 cut(s) 519
Kzo9I GATC 2 cut(s) 280, 429
LmnI GCTCC 1 cut(s) 541
LpnPI CCDG 4 cut(s) 192, 262, 389, 463
Lsp1109I GCAGC 4 cut(s) 10, 468, 496, 531
LweI GCATC 1 cut(s) 10
MaeI CTAG 1 cut(s) 296
MaeII ACGT 3 cut(s) 231, 310, 525
MaeIII GTNAC 2 cut(s) 512, 556
MalI GATC 2 cut(s) 282, 431
MboI GATC 2 cut(s) 280, 429
MboII GAAGA 2 cut(s) 29, 449
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 7 cut(s) 5, 170, 269, 348, 356, 472, 503
MmeI TCCRAC 1 cut(s) 40
MnlI CCTC 2 cut(s) 136, 158
MseI TTAA 2 cut(s) 359, 549
MslI CAYNNNNRTG 1 cut(s) 74
MspI CCGG 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 493
NdeII GATC 2 cut(s) 280, 429
NlaIII CATG 5 cut(s) 59, 73, 87, 492, 570
NmuCI GTSAC 2 cut(s) 512, 556
NsbI TGCGCA 1 cut(s) 520
NspI RCATGY 1 cut(s) 59
NspV TTCGAA 2 cut(s) 9, 184
PagI TCATGA 1 cut(s) 566
PcsI WCGNNNNNNNCGW 2 cut(s) 190, 316
PfeI GAWTC 3 cut(s) 11, 112, 226
PflMI CCANNNNNTGG 1 cut(s) 255
PkrI GCNGC 4 cut(s) 25, 483, 486, 546
PmaCI CACGTG 1 cut(s) 311
PmlI CACGTG 1 cut(s) 311
Ppu21I YACGTR 2 cut(s) 232, 311
PspCI CACGTG 1 cut(s) 311
PspEI GGTNACC 1 cut(s) 556
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 2 cut(s) 367, 486
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 359, 549
SatI GCNGC 4 cut(s) 24, 482, 485, 545
Sau3AI GATC 2 cut(s) 280, 429
Sau96I GGNCC 1 cut(s) 176
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 1 cut(s) 10
SfcI CTRYAG 2 cut(s) 363, 482
SfuI TTCGAA 2 cut(s) 9, 184
SmiMI CAYNNNNRTG 1 cut(s) 74
SpeI ACTAGT 1 cut(s) 295
Sse9I AATT 7 cut(s) 5, 170, 269, 348, 356, 472, 503
SspMI CTAG 1 cut(s) 296
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 152, 556
TaiI ACGT 3 cut(s) 234, 313, 528
TaqI TCGA 4 cut(s) 9, 124, 163, 184
TaqII GACCGA 1 cut(s) 81
TasI AATT 7 cut(s) 5, 170, 269, 348, 356, 472, 503
TatI WGTACW 1 cut(s) 200
TfiI GAWTC 3 cut(s) 11, 112, 226
Tru1I TTAA 2 cut(s) 359, 549
Tru9I TTAA 2 cut(s) 359, 549
TscAI CASTG 2 cut(s) 155, 540
TseFI GTSAC 2 cut(s) 512, 556
TseI GCWGC 4 cut(s) 23, 481, 484, 544
Tsp45I GTSAC 2 cut(s) 512, 556
TspDTI ATGAA 3 cut(s) 262, 462, 555
TspGWI ACGGA 1 cut(s) 500
TspRI CASTG 2 cut(s) 155, 540
Van91I CCANNNNNTGG 1 cut(s) 255
VneI GTGCAC 1 cut(s) 391
XapI RAATTY 4 cut(s) 5, 170, 269, 348
XceI RCATGY 1 cut(s) 59
XmiI GTMKAC 1 cut(s) 124
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.