MD04G1102400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010135
Physical Location & Seq
Reverse (-)
18915569 .. 18916758
1190 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1102400.v1.1.491

Sequence Viewer

Length: 285 bp
ATGGAGAAGCTTAAGAACTCTACAATCTTAACCACCTGCCTTTTTCTCTTCCTCCTTGCCAAACCTAGCTTTTCTAAAGTAGGTGGATCGGATTTGATGGATACTGACGTTTATGAGATTGATTATAGAGGTCCGGAGACACACTCATCAGTTCCGCCGCCTCATCATTATCATGGGAAGAAGCCTAAGCCATTGATTCACAAAGAAAACGCCATGGCAACCCCAAAACCTGAGAGCATTAAAGCTGCTAAGATGGGAAGAAAGGTGAAGAAAGTACATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

95

Amino Acids

10.55

Weight (kDa)

9.52

Isoelectric Point (pI)

30.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 2 cut(s) 155, 158
AclWI GGATC 1 cut(s) 94
AfaI GTAC 1 cut(s) 276
AflII CTTAAG 1 cut(s) 11
AluBI AGCT 3 cut(s) 10, 69, 245
AluI AGCT 3 cut(s) 10, 69, 245
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 94
Aor13HI TCCGGA 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 245
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 277
AvaII GGWCC 1 cut(s) 131
BaeI ACNNNNGTAYC 2 cut(s) 93, 126
BbvI GCAGC 1 cut(s) 232
BccI CCATC 2 cut(s) 91, 247
BciVI GTATCC 1 cut(s) 94
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 1 cut(s) 66
BfrI CTTAAG 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 44
BfuI GTATCC 1 cut(s) 94
BisI GCNGC 2 cut(s) 158, 246
BlsI GCNGC 2 cut(s) 159, 247
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BplI GAGNNNNNCTC 2 cut(s) 128, 160
Bpu10I CCTNAGC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 133
BseAI TCCGGA 1 cut(s) 133
BseDI CCNNGG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 232
BsiSI CCGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 131
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 1 cut(s) 86
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 2 cut(s) 155, 158
BspCNI CTCAG 1 cut(s) 223
BspEI TCCGGA 1 cut(s) 133
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 94
BspTI CTTAAG 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 213
Bst6I CTCTTC 1 cut(s) 53
BstAFI CTTAAG 1 cut(s) 11
BstDEI CTNAG 3 cut(s) 186, 231, 249
BstDSI CCRYGG 1 cut(s) 213
BstKTI GATC 1 cut(s) 89
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 232
BsuI GTATCC 1 cut(s) 94
BtgI CCRYGG 1 cut(s) 213
BveI ACCTGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 275
CviAII CATG 3 cut(s) 173, 214, 278
CviJI RGCY 5 cut(s) 10, 69, 184, 190, 245
CviKI_1 RGCY 5 cut(s) 10, 69, 184, 190, 245
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 3 cut(s) 186, 231, 249
DpnI GATC 1 cut(s) 88
DpnII GATC 1 cut(s) 86
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 3 cut(s) 176, 217, 281
FaiI YATR 5 cut(s) 114, 126, 174, 215, 279
FatI CATG 3 cut(s) 172, 213, 277
Fnu4HI GCNGC 2 cut(s) 158, 246
Fsp4HI GCNGC 2 cut(s) 158, 246
FspBI CTAG 1 cut(s) 66
GluI GCNGC 2 cut(s) 158, 246
HapII CCGG 1 cut(s) 134
Hin1II CATG 3 cut(s) 176, 217, 281
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 1 cut(s) 196
HpaII CCGG 1 cut(s) 134
HphI GGTGA 1 cut(s) 277
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 1 cut(s) 134
HpyCH4IV ACGT 1 cut(s) 108
HpyF3I CTNAG 3 cut(s) 186, 231, 249
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 3 cut(s) 176, 217, 281
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 1 cut(s) 86
LpnPI CCDG 3 cut(s) 49, 147, 243
Lsp1109I GCAGC 1 cut(s) 232
MaeI CTAG 1 cut(s) 66
MaeII ACGT 1 cut(s) 108
MalI GATC 1 cut(s) 88
MboI GATC 1 cut(s) 86
MboII GAAGA 4 cut(s) 40, 190, 270, 280
MnlI CCTC 3 cut(s) 62, 122, 171
MroI TCCGGA 1 cut(s) 133
MseI TTAA 3 cut(s) 12, 29, 240
MslI CAYNNNNRTG 1 cut(s) 171
MspCI CTTAAG 1 cut(s) 11
MspI CCGG 1 cut(s) 134
NcoI CCATGG 1 cut(s) 213
NdeII GATC 1 cut(s) 86
NlaIII CATG 3 cut(s) 176, 217, 281
PaqCI CACCTGC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 196
PkrI GCNGC 2 cut(s) 159, 247
PspPI GGNCC 1 cut(s) 131
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
RseI CAYNNNNRTG 1 cut(s) 171
SaqAI TTAA 3 cut(s) 12, 29, 240
SatI GCNGC 2 cut(s) 158, 246
Sau3AI GATC 1 cut(s) 86
Sau96I GGNCC 1 cut(s) 131
SgeI CNNG 7 cut(s) 48, 68, 78, 146, 185, 226, 242
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
SsiI CCGC 2 cut(s) 155, 158
SspMI CTAG 1 cut(s) 66
StyI CCWWGG 1 cut(s) 213
TaiI ACGT 1 cut(s) 111
TatI WGTACW 1 cut(s) 274
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 3 cut(s) 12, 29, 240
Tru9I TTAA 3 cut(s) 12, 29, 240
TseI GCWGC 1 cut(s) 245
Vha464I CTTAAG 1 cut(s) 11
VpaK11BI GGWCC 1 cut(s) 131
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.