pycom12g12110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010135
Physical Location & Seq
Reverse (-)
14617003 .. 14618569
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g12110.2

Sequence Viewer

Length: 297 bp
ATGGAGAAGCTCAAGAACTCTACAATCTTCACCACCTGCCTTTTTCTCTTCCTCCTTGCCACACCTTGCTTTTCCAAAGGTGAATCGGATTTGATGGATACTGAGGTTTATGAAATCGATTATAGAGGTCCGGAGACTCACTCATCAGTTCCACCACCTGGTCACTCTCATGGGAAGAAGCCTTTGCCTTTGATCCACAAAGAAAATGCCATGGCAACCCCAAAACCTAAGAGCATTGAAGGTGCTAACATGGGAAGAAAGAAAGTGGGATCCGGATCAAACCACGTCAACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

99

Amino Acids

10.74

Weight (kDa)

8.6

Isoelectric Point (pI)

27.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AccB7I CCANNNNNTGG 1 cut(s) 158
AccIII TCCGGA 2 cut(s) 130, 272
AclWI GGATC 4 cut(s) 187, 264, 277, 283
AfiI CCNNNNNNNGG 1 cut(s) 158
AgsI TTSAA 1 cut(s) 239
AjiI CACGTC 1 cut(s) 286
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 1 cut(s) 10
AluI AGCT 1 cut(s) 10
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 4 cut(s) 187, 264, 277, 283
Aor13HI TCCGGA 2 cut(s) 130, 272
AspS9I GGNCC 1 cut(s) 128
AsuHPI GGTGA 2 cut(s) 22, 92
AvaII GGWCC 1 cut(s) 128
BaeI ACNNNNGTAYC 2 cut(s) 90, 123
BamHI GGATCC 1 cut(s) 269
BccI CCATC 1 cut(s) 88
BciT130I CCWGG 1 cut(s) 159
BciVI GTATCC 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 44
BfuI GTATCC 1 cut(s) 91
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 271
BmrFI CCNGG 1 cut(s) 159
BplI GAGNNNNNCTC 2 cut(s) 125, 157
Bsa29I ATCGAT 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 274
BsaJI CCNNGG 1 cut(s) 210
BsaWI WCCGGW 2 cut(s) 130, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse8I GATNNNNATC 1 cut(s) 274
BseAI TCCGGA 2 cut(s) 130, 272
BseBI CCWGG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 210
BseJI GATNNNNATC 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 93
BshVI ATCGAT 1 cut(s) 117
BsiSI CCGG 2 cut(s) 131, 273
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 1 cut(s) 128
Bsp13I TCCGGA 2 cut(s) 130, 272
Bsp143I GATC 3 cut(s) 192, 269, 275
Bsp19I CCATGG 1 cut(s) 210
BspCNI CTCAG 1 cut(s) 94
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 2 cut(s) 130, 272
BspLI GGNNCC 1 cut(s) 271
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 4 cut(s) 187, 264, 277, 283
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 3 cut(s) 192, 269, 275
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 159
Bst6I CTCTTC 1 cut(s) 53
BstDEI CTNAG 2 cut(s) 102, 228
BstDSI CCRYGG 1 cut(s) 210
BstKTI GATC 3 cut(s) 195, 272, 278
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 3 cut(s) 192, 269, 275
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstX2I RGATCY 1 cut(s) 269
BstYI RGATCY 1 cut(s) 269
Bsu15I ATCGAT 1 cut(s) 117
BsuI GTATCC 1 cut(s) 91
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 210
BtrI CACGTC 1 cut(s) 286
BveI ACCTGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 128
ClaI ATCGAT 1 cut(s) 117
CsiI ACCWGGT 1 cut(s) 157
CviAII CATG 3 cut(s) 170, 211, 250
CviJI RGCY 2 cut(s) 10, 181
CviKI_1 RGCY 2 cut(s) 10, 181
DdeI CTNAG 2 cut(s) 102, 228
DpnI GATC 3 cut(s) 194, 271, 277
DpnII GATC 3 cut(s) 192, 269, 275
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 3 cut(s) 173, 214, 253
FaiI YATR 5 cut(s) 111, 123, 171, 212, 251
FatI CATG 3 cut(s) 169, 210, 249
HapII CCGG 2 cut(s) 131, 273
Hin1II CATG 3 cut(s) 173, 214, 253
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 2 cut(s) 83, 136
HpaII CCGG 2 cut(s) 131, 273
HphI GGTGA 2 cut(s) 22, 92
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 3 cut(s) 13, 131, 273
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 1 cut(s) 233
HpyCH4IV ACGT 1 cut(s) 285
HpyF3I CTNAG 2 cut(s) 102, 228
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 3 cut(s) 173, 214, 253
Kpn2I TCCGGA 2 cut(s) 130, 272
Kzo9I GATC 3 cut(s) 192, 269, 275
LpnPI CCDG 5 cut(s) 49, 144, 144, 171, 286
MabI ACCWGGT 1 cut(s) 157
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 161
MalI GATC 3 cut(s) 194, 271, 277
MboI GATC 3 cut(s) 192, 269, 275
MboII GAAGA 4 cut(s) 19, 40, 187, 267
MflI RGATCY 1 cut(s) 269
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 3 cut(s) 62, 97, 119
MroI TCCGGA 2 cut(s) 130, 272
MseI TTAA 1 cut(s) 295
MslI CAYNNNNRTG 1 cut(s) 168
MspI CCGG 2 cut(s) 131, 273
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
NcoI CCATGG 1 cut(s) 210
NdeII GATC 3 cut(s) 192, 269, 275
NlaIII CATG 3 cut(s) 173, 214, 253
NlaIV GGNNCC 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 161
PaqCI CACCTGC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 158
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 271
PspPI GGNCC 1 cut(s) 128
PsuI RGATCY 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 1 cut(s) 295
Sau3AI GATC 3 cut(s) 192, 269, 275
Sau96I GGNCC 1 cut(s) 128
SchI GAGTC 1 cut(s) 130
ScrFI CCNGG 1 cut(s) 159
SexAI ACCWGGT 1 cut(s) 157
SinI GGWCC 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 1 cut(s) 210
TaiI ACGT 1 cut(s) 288
TaqI TCGA 1 cut(s) 117
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 295
Tru9I TTAA 1 cut(s) 295
TseFI GTSAC 1 cut(s) 161
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 1 cut(s) 126
Van91I CCANNNNNTGG 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.