MD04G1123900.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005672
Physical Location & Seq
Reverse (-)
21002121 .. 21002342
222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1123900.v1.1.491

Sequence Viewer

Length: 222 bp
ATGGCTAGTGGCAAGCAAATTGTTGGTGAAGACAAGCAGCGTGCCCGGACAGAGATCGTGTACGGCCCCGAAGATTGCTACCAGCAGTCCATAGACCTACTAGAAGAGCTAGGGTTTCCCAAAGGCATCCTCCCTCTGCAGGACCTCGTGGAGTGCGGGAGGGTCAGAGAAACTGGGTTCGTGTGGATGAAGCAGAAGCAACCCTACGAGCACTTTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

74

Amino Acids

8.48

Weight (kDa)

5.02

Isoelectric Point (pI)

61.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 32 - 70 3.2e-08 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 156
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 1 cut(s) 139
AluBI AGCT 1 cut(s) 109
AluI AGCT 1 cut(s) 109
Alw21I GWGCWC 1 cut(s) 213
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 65, 142
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 38
AvaII GGWCC 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 46
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 49
BceAI ACGGC 1 cut(s) 79
BcnI CCSGG 1 cut(s) 46
BfaI CTAG 3 cut(s) 6, 101, 110
BfmI CTRYAG 1 cut(s) 137
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 65, 142
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 1 cut(s) 46
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 1 cut(s) 135
BmuI ACTGGG 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 36
BpuMI CCSGG 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 178
BseGI GGATG 2 cut(s) 126, 192
BseLI CCNNNNNNNGG 1 cut(s) 139
BseNI ACTGG 1 cut(s) 178
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 1 cut(s) 49
BshFI GGCC 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 139
BsnI GGCC 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 46, 213
Bsp143I GATC 1 cut(s) 54
BspACI CCGC 1 cut(s) 156
BspANI GGCC 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 67
BspMAI CTGCAG 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 99
BsrI ACTGG 1 cut(s) 178
BssMI GATC 1 cut(s) 54
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 14, 42
BstF5I GGATG 2 cut(s) 126, 192
BstKTI GATC 1 cut(s) 57
BstMBI GATC 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 44
BstSFI CTRYAG 1 cut(s) 137
BstSLI GKGCMC 1 cut(s) 46
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 36
BsuRI GGCC 1 cut(s) 66
BtsCI GGATG 2 cut(s) 126, 192
Cac8I GCNNGC 2 cut(s) 14, 42
Cfr13I GGNCC 2 cut(s) 65, 142
Csp6I GTAC 1 cut(s) 61
CviJI RGCY 3 cut(s) 5, 66, 109
CviKI_1 RGCY 3 cut(s) 5, 66, 109
CviQI GTAC 1 cut(s) 61
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 142
FaiI YATR 1 cut(s) 92
FauI CCCGC 1 cut(s) 149
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 2 cut(s) 113, 199
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 3 cut(s) 6, 101, 110
GluI GCNGC 1 cut(s) 38
HaeIII GGCC 1 cut(s) 66
HapII CCGG 1 cut(s) 46
HpaII CCGG 1 cut(s) 46
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 61
HpyCH4V TGCA 1 cut(s) 139
Kzo9I GATC 1 cut(s) 54
LguI GCTCTTC 1 cut(s) 99
LpnPI CCDG 4 cut(s) 59, 95, 125, 159
Lsp1109I GCAGC 1 cut(s) 49
LweI GCATC 1 cut(s) 135
MaeI CTAG 3 cut(s) 6, 101, 110
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 3 cut(s) 41, 83, 116
MhlI GDGCHC 2 cut(s) 46, 213
MluCI AATT 1 cut(s) 18
MnlI CCTC 4 cut(s) 140, 144, 153, 155
MspI CCGG 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 46
NciI CCSGG 1 cut(s) 46
NdeII GATC 1 cut(s) 54
NlaIV GGNNCC 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 99
PkrI GCNGC 1 cut(s) 39
PpuMI RGGWCCY 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 2 cut(s) 65, 142
PspPPI RGGWCCY 1 cut(s) 142
PstI CTGCAG 1 cut(s) 141
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 99
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 65, 142
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 2 cut(s) 46, 213
SetI ASST 3 cut(s) 99, 111, 147
SfaNI GCATC 1 cut(s) 135
SfcI CTRYAG 1 cut(s) 137
SinI GGWCC 1 cut(s) 142
Sse9I AATT 1 cut(s) 18
SsiI CCGC 1 cut(s) 156
SspMI CTAG 3 cut(s) 6, 101, 110
StyD4I CCNGG 1 cut(s) 44
TasI AATT 1 cut(s) 18
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 142
XspI CTAG 3 cut(s) 6, 101, 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.