Prupe.5G129600_v2.0.a1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0005672
Physical Location & Seq
Forward (+)
12538025 .. 12539405
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G129600.1

Sequence Viewer

Length: 465 bp
ATGGCTAGTAACAAGCAGCTGGAAGACAAGCGTGCAGGTGCAGAGATTGTTTACGGCCCCGAAGATTGCTACCGCCACTCCATAGACCTCCTGGAAGAGCTAGGGTTTCCCAAAGGTGTCCTGCCCCTGCAGGACCTTGTGGAGTGTGGGAGGGTGAGAGAAACTGGGTTTGTGTGGATGAAGCAAAAGCGGCCATATGAGCACTTCTTTGTTGGGTCCAATACACGTGTCAGCTATGCCACTGAGGTGACTGGTTATGTGGAGAAGTTGAAGATGAAGAAGATGACTGGGATTAAGAGCAAGCAGGTTTTTCTTTGGGTCCCAATTTCTGAGATGAGCATTGACGACCCGGCAAGCAAAAAGATTGTCTTCAAGACCCCAATGGGGATTGGAAAGTCTTTTCCAATCACAGCTTTCATGACTGAAGAGGAGAAGCAGAAGCATTTGGAGCAGGTGAATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

155

Amino Acids

17.65

Weight (kDa)

7.73

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 26, 442
Acc36I ACCTGC 3 cut(s) 26, 295, 442
AciI CCGC 2 cut(s) 73, 190
AcoI YGGCCR 1 cut(s) 191
AcuI CTGAAG 1 cut(s) 444
AcvI CACGTG 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 384
AflIII ACRYGT 2 cut(s) 224, 226
AgsI TTSAA 2 cut(s) 271, 373
AjnI CCWGG 1 cut(s) 90
AleI CACNNNNGTG 1 cut(s) 245
AluBI AGCT 4 cut(s) 19, 100, 234, 413
AluI AGCT 4 cut(s) 19, 100, 234, 413
Alw21I GWGCWC 1 cut(s) 204
AoxI GGCC 2 cut(s) 55, 191
ApeKI GCWGC 1 cut(s) 16
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
AspS9I GGNCC 4 cut(s) 56, 133, 216, 319
AsuC2I CCSGG 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 166, 259
AvaII GGWCC 3 cut(s) 133, 216, 319
BbrPI CACGTG 1 cut(s) 227
BbsI GAAGAC 2 cut(s) 30, 361
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 28
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 1 cut(s) 92
BcnI CCSGG 1 cut(s) 350
BfaI CTAG 2 cut(s) 6, 101
BfmI CTRYAG 1 cut(s) 128
BfuAI ACCTGC 3 cut(s) 26, 295, 442
BisI GCNGC 2 cut(s) 17, 191
BlsI GCNGC 2 cut(s) 18, 192
Bme1390I CCNGG 2 cut(s) 92, 350
Bme18I GGWCC 3 cut(s) 133, 216, 319
BmgT120I GGNCC 4 cut(s) 56, 133, 216, 319
BmiI GGNNCC 4 cut(s) 58, 217, 320, 321
BmrFI CCNGG 2 cut(s) 92, 350
BmrI ACTGGG 2 cut(s) 174, 297
BmuI ACTGGG 2 cut(s) 174, 297
BpiI GAAGAC 2 cut(s) 30, 361
BpuMI CCSGG 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse1I ACTGG 3 cut(s) 169, 256, 292
BseBI CCWGG 1 cut(s) 92
BseGI GGATG 1 cut(s) 183
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMII CTCAG 2 cut(s) 234, 321
BseNI ACTGG 3 cut(s) 169, 256, 292
BseRI GAGGAG 1 cut(s) 443
BseXI GCAGC 1 cut(s) 28
BsgI GTGCAG 2 cut(s) 54, 60
BshFI GGCC 2 cut(s) 57, 193
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 2 cut(s) 57, 193
Bsp1286I GDGCHC 1 cut(s) 204
BspACI CCGC 2 cut(s) 73, 190
BspANI GGCC 2 cut(s) 57, 193
BspCNI CTCAG 2 cut(s) 235, 322
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 4 cut(s) 58, 217, 320, 321
BspMAI CTGCAG 1 cut(s) 132
BspMI ACCTGC 3 cut(s) 26, 295, 442
BspQI GCTCTTC 1 cut(s) 90
BsrI ACTGG 3 cut(s) 169, 256, 292
Bst2UI CCWGG 1 cut(s) 92
Bst6I CTCTTC 2 cut(s) 90, 420
BstBAI YACGTR 1 cut(s) 227
BstC8I GCNNGC 3 cut(s) 33, 302, 355
BstDEI CTNAG 2 cut(s) 243, 330
BstF5I GGATG 1 cut(s) 183
BstMWI GCNNNNNNNGC 3 cut(s) 190, 199, 448
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 90, 348
BstSFI CTRYAG 1 cut(s) 128
BstV1I GCAGC 1 cut(s) 28
BstV2I GAAGAC 2 cut(s) 30, 361
BsuRI GGCC 2 cut(s) 57, 193
BtsCI GGATG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 240
BveI ACCTGC 3 cut(s) 26, 295, 442
Cac8I GCNNGC 3 cut(s) 33, 302, 355
CciI TCATGA 1 cut(s) 417
Cfr13I GGNCC 4 cut(s) 56, 133, 216, 319
CviAII CATG 1 cut(s) 418
CviJI RGCY 7 cut(s) 5, 19, 57, 100, 193, 234, 413
CviKI_1 RGCY 7 cut(s) 5, 19, 57, 100, 193, 234, 413
DdeI CTNAG 2 cut(s) 243, 330
EaeI YGGCCR 1 cut(s) 191
Eam1104I CTCTTC 2 cut(s) 90, 420
EarI CTCTTC 2 cut(s) 90, 420
Eco47I GGWCC 3 cut(s) 133, 216, 319
Eco57I CTGAAG 1 cut(s) 444
Eco72I CACGTG 1 cut(s) 227
EcoO109I RGGNCCY 2 cut(s) 133, 319
EcoRII CCWGG 1 cut(s) 90
FaeI CATG 1 cut(s) 421
FaiI YATR 6 cut(s) 83, 196, 198, 237, 258, 419
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FaqI GGGAC 1 cut(s) 305
FatI CATG 1 cut(s) 417
FauNDI CATATG 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 17, 191
FokI GGATG 1 cut(s) 190
Fsp4HI GCNGC 2 cut(s) 17, 191
FspBI CTAG 2 cut(s) 6, 101
GluI GCNGC 2 cut(s) 17, 191
HaeIII GGCC 2 cut(s) 57, 193
HapII CCGG 1 cut(s) 350
Hin1II CATG 1 cut(s) 421
HpaII CCGG 1 cut(s) 350
HphI GGTGA 2 cut(s) 166, 259
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 331
Hpy188III TCNNGA 2 cut(s) 373, 418
Hpy8I GTNNAC 1 cut(s) 52
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 3 cut(s) 35, 41, 130
HpyF10VI GCNNNNNNNGC 3 cut(s) 190, 199, 448
HpyF3I CTNAG 2 cut(s) 243, 330
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 1 cut(s) 421
KflI GGGWCCC 1 cut(s) 319
LguI GCTCTTC 1 cut(s) 90
LmnI GCTCC 1 cut(s) 448
Lsp1109I GCAGC 1 cut(s) 28
MaeI CTAG 2 cut(s) 6, 101
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 2 cut(s) 8, 247
MboII GAAGA 8 cut(s) 35, 74, 107, 283, 289, 292, 361, 437
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 1 cut(s) 324
MnlI CCTC 4 cut(s) 98, 144, 238, 421
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 245
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 2 cut(s) 92, 350
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 190, 199, 448
NciI CCSGG 1 cut(s) 350
NdeI CATATG 1 cut(s) 196
NlaIII CATG 1 cut(s) 421
NlaIV GGNNCC 4 cut(s) 58, 217, 320, 321
NmuCI GTSAC 1 cut(s) 247
OliI CACNNNNGTG 1 cut(s) 245
PagI TCATGA 1 cut(s) 417
PaqCI CACCTGC 2 cut(s) 26, 442
PciSI GCTCTTC 1 cut(s) 90
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 2 cut(s) 18, 192
PmaCI CACGTG 1 cut(s) 227
PmlI CACGTG 1 cut(s) 227
Ppu21I YACGTR 1 cut(s) 227
PpuMI RGGWCCY 2 cut(s) 133, 319
Psp5II RGGWCCY 2 cut(s) 133, 319
Psp6I CCWGG 1 cut(s) 90
PspCI CACGTG 1 cut(s) 227
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 4 cut(s) 58, 217, 320, 321
PspPI GGNCC 4 cut(s) 56, 133, 216, 319
PspPPI RGGWCCY 2 cut(s) 133, 319
PstI CTGCAG 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 19
RseI CAYNNNNRTG 1 cut(s) 245
SapI GCTCTTC 1 cut(s) 90
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 2 cut(s) 17, 191
Sau96I GGNCC 4 cut(s) 56, 133, 216, 319
SbfI CCTGCAGG 1 cut(s) 132
ScrFI CCNGG 2 cut(s) 92, 350
SdaI CCTGCAGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 204
SfcI CTRYAG 1 cut(s) 128
SinI GGWCC 3 cut(s) 133, 216, 319
SmiMI CAYNNNNRTG 1 cut(s) 245
Sse8387I CCTGCAGG 1 cut(s) 132
Sse9I AATT 1 cut(s) 324
SsiI CCGC 2 cut(s) 73, 190
SspMI CTAG 2 cut(s) 6, 101
StyD4I CCNGG 2 cut(s) 90, 348
TaiI ACGT 1 cut(s) 229
TasI AATT 1 cut(s) 324
TauI GCSGC 1 cut(s) 193
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 3 cut(s) 194, 290, 406
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 3 cut(s) 133, 216, 319
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 2 cut(s) 6, 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.