Rmu_sc0000809.1_g000034

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000809.1
OG0005672
Physical Location & Seq
Reverse (-)
151745 .. 152200
456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000809.1_g000034.1.cds

Sequence Viewer

Length: 456 bp
atggctcttgtggaagacaagcgcgcaggggctgagattgtgcatggagctcaagagtgctgccaacagtccatagatctcttggaagagctgggatttcccaaaggtgttcttcccctgcaagacctggtggagtgtggaagggtgaaggaaactgggtttgtgtggatgaagcagaaggcgccatacgagcacttcttcgttgggtcgaacacgcgtgtgagctacgcgactgaggtgactgcgtatgtggagaagggcaggatgaagaagatgactgggatcaagagcaagcaggtgtttctttgggtgcctattagtgagatgatcatggaagagccagcaaccaagaagatcactttcaatactccaatggggcttggaaggacttttccggtcacggctttcatgaccgaggaggagaagcagaagtatctggagaatagtaatgagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

151

Amino Acids

17.13

Weight (kDa)

5.81

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 286
AccB1I GGYRCC 2 cut(s) 181, 310
AccII CGCG 3 cut(s) 24, 217, 230
AclWI GGATC 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 1 cut(s) 364
AjnI CCWGG 1 cut(s) 126
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 3 cut(s) 50, 91, 225
AluI AGCT 3 cut(s) 50, 91, 225
Alw21I GWGCWC 2 cut(s) 52, 195
AlwI GGATC 1 cut(s) 290
AlwNI CAGNNNCTG 1 cut(s) 32
ApeKI GCWGC 1 cut(s) 60
AspLEI GCGC 3 cut(s) 24, 26, 184
AsuHPI GGTGA 2 cut(s) 157, 250
BanI GGYRCC 2 cut(s) 181, 310
BanII GRGCYC 1 cut(s) 52
BarI GAAGNNNNNNTAC 2 cut(s) 170, 202
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 2 cut(s) 52, 195
BbvI GCAGC 1 cut(s) 47
BceAI ACGGC 1 cut(s) 417
BciT130I CCWGG 1 cut(s) 128
BclI TGATCA 1 cut(s) 327
BfoI RGCGCY 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 76
BisI GCNGC 1 cut(s) 61
BlsI GCNGC 1 cut(s) 62
Bme1390I CCNGG 1 cut(s) 128
BmiI GGNNCC 2 cut(s) 183, 312
BmrFI CCNGG 1 cut(s) 128
BmrI ACTGGG 2 cut(s) 165, 288
BmuI ACTGGG 2 cut(s) 165, 288
BpiI GAAGAC 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 414
BsaWI WCCGGW 1 cut(s) 394
Bse1I ACTGG 2 cut(s) 160, 283
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 2 cut(s) 174, 270
BseMII CTCAG 2 cut(s) 24, 225
BseNI ACTGG 2 cut(s) 160, 283
BsePI GCGCGC 1 cut(s) 22
BseRI GAGGAG 2 cut(s) 431, 434
BseXI GCAGC 1 cut(s) 47
BseYI CCCAGC 1 cut(s) 91
Bsh1236I CGCG 3 cut(s) 24, 217, 230
BshNI GGYRCC 2 cut(s) 181, 310
BsiHKAI GWGCWC 2 cut(s) 52, 195
BsiSI CCGG 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 52, 195
Bsp143I GATC 4 cut(s) 76, 282, 327, 354
BspCNI CTCAG 2 cut(s) 25, 226
BspFNI CGCG 3 cut(s) 24, 217, 230
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 2 cut(s) 183, 312
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 1 cut(s) 290
BspQI GCTCTTC 2 cut(s) 81, 330
BspT107I GGYRCC 2 cut(s) 181, 310
BsrI ACTGG 2 cut(s) 160, 283
BssECI CCNNGG 1 cut(s) 414
BssHII GCGCGC 1 cut(s) 22
BssMI GATC 4 cut(s) 76, 282, 327, 354
BssNI GRCGYC 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 69
Bst6I CTCTTC 2 cut(s) 81, 330
BstACI GRCGYC 1 cut(s) 182
BstC8I GCNNGC 3 cut(s) 24, 293, 342
BstDEI CTNAG 2 cut(s) 33, 234
BstF5I GGATG 2 cut(s) 174, 270
BstFNI CGCG 3 cut(s) 24, 217, 230
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 3 cut(s) 24, 26, 184
BstKTI GATC 4 cut(s) 79, 285, 330, 357
BstMBI GATC 4 cut(s) 76, 282, 327, 354
BstMWI GCNNNNNNNGC 2 cut(s) 181, 190
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstUI CGCG 3 cut(s) 24, 217, 230
BstV1I GCAGC 1 cut(s) 47
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BtsCI GGATG 2 cut(s) 174, 270
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 24, 293, 342
CaiI CAGNNNCTG 1 cut(s) 32
CciI TCATGA 1 cut(s) 408
CfoI GCGC 3 cut(s) 24, 26, 184
CsiI ACCWGGT 1 cut(s) 126
CviAII CATG 3 cut(s) 44, 331, 409
CviJI RGCY 8 cut(s) 5, 32, 50, 91, 225, 340, 379, 404
CviKI_1 RGCY 8 cut(s) 5, 32, 50, 91, 225, 340, 379, 404
DdeI CTNAG 2 cut(s) 33, 234
DinI GGCGCC 1 cut(s) 183
DpnI GATC 4 cut(s) 78, 284, 329, 356
DpnII GATC 4 cut(s) 76, 282, 327, 354
Eam1104I CTCTTC 2 cut(s) 81, 330
EarI CTCTTC 2 cut(s) 81, 330
Ecl136II GAGCTC 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 52
EgeI GGCGCC 1 cut(s) 183
EheI GGCGCC 1 cut(s) 183
FaeI CATG 3 cut(s) 47, 334, 412
FaiI YATR 6 cut(s) 45, 74, 187, 249, 332, 410
FalI AAGNNNNNCTT 2 cut(s) 96, 128
FatI CATG 3 cut(s) 43, 330, 408
FbaI TGATCA 1 cut(s) 327
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 2 cut(s) 181, 277
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 1 cut(s) 61
GlaI GCGC 3 cut(s) 23, 25, 183
GluI GCNGC 1 cut(s) 61
GsaI CCCAGC 1 cut(s) 95
HaeII RGCGCY 1 cut(s) 185
HapII CCGG 1 cut(s) 395
HhaI GCGC 3 cut(s) 24, 26, 184
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 3 cut(s) 47, 334, 412
Hin6I GCGC 3 cut(s) 22, 24, 182
HinP1I GCGC 3 cut(s) 22, 24, 182
HpaII CCGG 1 cut(s) 395
HphI GGTGA 2 cut(s) 157, 250
Hpy188III TCNNGA 4 cut(s) 53, 286, 409, 437
HpyAV CCTTC 5 cut(s) 135, 142, 172, 250, 378
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 43, 121
HpyF10VI GCNNNNNNNGC 2 cut(s) 181, 190
HpyF3I CTNAG 2 cut(s) 33, 234
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 3 cut(s) 47, 334, 412
HspAI GCGC 3 cut(s) 22, 24, 182
KasI GGCGCC 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 4 cut(s) 76, 282, 327, 354
LguI GCTCTTC 2 cut(s) 81, 330
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 1 cut(s) 47
MabI ACCWGGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 238, 397
MalI GATC 4 cut(s) 78, 284, 329, 356
MboI GATC 4 cut(s) 76, 282, 327, 354
MboII GAAGA 8 cut(s) 26, 98, 104, 190, 280, 283, 347, 364
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 2 cut(s) 52, 195
MluI ACGCGT 1 cut(s) 215
Mly113I GGCGCC 1 cut(s) 182
MnlI CCTC 3 cut(s) 229, 409, 412
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 3 cut(s) 24, 217, 230
MwoI GCNNNNNNNGC 2 cut(s) 181, 190
NarI GGCGCC 1 cut(s) 182
NdeII GATC 4 cut(s) 76, 282, 327, 354
NlaIII CATG 3 cut(s) 47, 334, 412
NlaIV GGNNCC 2 cut(s) 183, 312
NmuCI GTSAC 2 cut(s) 238, 397
OliI CACNNNNGTG 1 cut(s) 218
PagI TCATGA 1 cut(s) 408
PaqCI CACCTGC 1 cut(s) 286
PauI GCGCGC 1 cut(s) 22
PciSI GCTCTTC 2 cut(s) 81, 330
PkrI GCNGC 1 cut(s) 62
PluTI GGCGCC 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 126
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 183, 312
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 1 cut(s) 76
PteI GCGCGC 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 218
SacI GAGCTC 1 cut(s) 52
SapI GCTCTTC 2 cut(s) 81, 330
SatI GCNGC 1 cut(s) 61
Sau3AI GATC 4 cut(s) 76, 282, 327, 354
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 2 cut(s) 52, 195
SetI ASST 7 cut(s) 52, 93, 109, 129, 227, 240, 300
SexAI ACCWGGT 1 cut(s) 126
SfoI GGCGCC 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
SspDI GGCGCC 1 cut(s) 181
SstI GAGCTC 1 cut(s) 52
StyD4I CCNGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 209
TaqII GACCGA 1 cut(s) 428
TseFI GTSAC 2 cut(s) 238, 397
TseI GCWGC 1 cut(s) 60
Tsp45I GTSAC 2 cut(s) 238, 397
TspDTI ATGAA 3 cut(s) 185, 281, 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.