pycom06g11000

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005672
Physical Location & Seq
Forward (+)
16193527 .. 16194000
474 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g11000.1

Sequence Viewer

Length: 474 bp
ATGGCTAGTGGCAAGCAAATTGTTGGTGAAGACAAGCAGCGTGCCGGGGCGGAGATCGTGTACGGCCCCGAAGATTGCTACCGGCAGTCCATAGACCTACTGGAAGAGCTAGGGTTTCCCAAAGGCGTCCTCCCCTTGCAGGACCTCGTGGAGTGCGGGAGGGTCAGAGAAACTGGGTTCGTGTGGATGAAGCAGAAGCAACCCTACGAGCACTTCTTTGAGGGGTCCAACACGCGTGTCAGCTACGCAACTGAGGTGACTGGGTACGTGGAGAAGTGCAGGATGAAGAAGATGACAGGGATCAAGAGCAAGCAGGTTTTTCTTTGGGTCCCGATATCTGAGATGAGCGTTGAGGATCCGGCGGGGAAGAAGATCGTTTTCAAGACTCCGTTGTGGATCGGGAAGTCTTTTCCGGTGACTGCGTTCATGACGGAGGAGGAGAAGCAAAAGTATTTGGAGAAGGTTAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

158

Amino Acids

17.96

Weight (kDa)

6.63

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 32 - 142 2.3e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 304
AccII CGCG 1 cut(s) 235
AciI CCGC 3 cut(s) 50, 156, 362
AclWI GGATC 4 cut(s) 308, 350, 363, 404
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 2 cut(s) 62, 266
AfiI CCNNNNNNNGG 1 cut(s) 139
AflIII ACRYGT 1 cut(s) 233
AgsI TTSAA 1 cut(s) 382
AluBI AGCT 2 cut(s) 109, 243
AluI AGCT 2 cut(s) 109, 243
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 4 cut(s) 308, 350, 363, 404
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 1 cut(s) 37
AspS9I GGNCC 4 cut(s) 65, 142, 225, 328
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 3 cut(s) 38, 268, 427
AvaII GGWCC 3 cut(s) 142, 225, 328
BamHI GGATCC 1 cut(s) 355
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 49
BceAI ACGGC 1 cut(s) 79
BcnI CCSGG 1 cut(s) 46
BfaI CTAG 2 cut(s) 6, 110
BfuAI ACCTGC 1 cut(s) 304
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 3 cut(s) 142, 225, 328
BmgT120I GGNCC 4 cut(s) 65, 142, 225, 328
BmiI GGNNCC 5 cut(s) 67, 226, 329, 330, 357
BmrFI CCNGG 1 cut(s) 46
BmrI ACTGGG 2 cut(s) 183, 270
BmuI ACTGGG 2 cut(s) 183, 270
BpiI GAAGAC 1 cut(s) 36
BpuMI CCSGG 1 cut(s) 46
BsaAI YACGTR 1 cut(s) 268
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse118I RCCGGY 1 cut(s) 81
Bse1I ACTGG 3 cut(s) 105, 178, 265
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 2 cut(s) 192, 288
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMII CTCAG 2 cut(s) 243, 330
BseNI ACTGG 3 cut(s) 105, 178, 265
BseRI GAGGAG 2 cut(s) 449, 452
BseXI GCAGC 1 cut(s) 49
BsgI GTGCAG 1 cut(s) 298
Bsh1236I CGCG 1 cut(s) 235
BshFI GGCC 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 4 cut(s) 45, 82, 359, 413
BslFI GGGAC 1 cut(s) 314
BslI CCNNNNNNNGG 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 314
BsnI GGCC 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 5 cut(s) 54, 300, 355, 372, 396
BspACI CCGC 3 cut(s) 50, 156, 362
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 2 cut(s) 244, 331
BspFNI CGCG 1 cut(s) 235
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 5 cut(s) 67, 226, 329, 330, 357
BspMI ACCTGC 1 cut(s) 304
BspPI GGATC 4 cut(s) 308, 350, 363, 404
BspQI GCTCTTC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 81
BsrI ACTGG 3 cut(s) 105, 178, 265
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 5 cut(s) 54, 300, 355, 372, 396
BssNI GRCGYC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 99
BstACI GRCGYC 1 cut(s) 126
BstBAI YACGTR 1 cut(s) 268
BstC8I GCNNGC 3 cut(s) 14, 42, 311
BstDEI CTNAG 2 cut(s) 252, 339
BstF5I GGATG 2 cut(s) 192, 288
BstFNI CGCG 1 cut(s) 235
BstKTI GATC 5 cut(s) 57, 303, 358, 375, 399
BstMBI GATC 5 cut(s) 54, 300, 355, 372, 396
BstSCI CCNGG 1 cut(s) 44
BstUI CGCG 1 cut(s) 235
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 1 cut(s) 66
BtsCI GGATG 2 cut(s) 192, 288
BveI ACCTGC 1 cut(s) 304
Cac8I GCNNGC 3 cut(s) 14, 42, 311
CciI TCATGA 1 cut(s) 426
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 4 cut(s) 65, 142, 225, 328
CseI GACGC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 61, 265
CviAII CATG 1 cut(s) 427
CviJI RGCY 4 cut(s) 5, 66, 109, 243
CviKI_1 RGCY 4 cut(s) 5, 66, 109, 243
CviQI GTAC 2 cut(s) 61, 265
DdeI CTNAG 2 cut(s) 252, 339
DpnI GATC 5 cut(s) 56, 302, 357, 374, 398
DpnII GATC 5 cut(s) 54, 300, 355, 372, 396
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 65
Eco32I GATATC 1 cut(s) 336
Eco47I GGWCC 3 cut(s) 142, 225, 328
EcoO109I RGGNCCY 2 cut(s) 142, 328
EcoRV GATATC 1 cut(s) 336
FaeI CATG 1 cut(s) 430
FaiI YATR 2 cut(s) 92, 428
FaqI GGGAC 1 cut(s) 314
FatI CATG 1 cut(s) 426
FauI CCCGC 2 cut(s) 149, 355
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 2 cut(s) 199, 295
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 2 cut(s) 6, 110
GluI GCNGC 1 cut(s) 38
HaeIII GGCC 1 cut(s) 66
HapII CCGG 4 cut(s) 45, 82, 359, 413
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 1 cut(s) 430
HinfI GANTC 1 cut(s) 385
HpaII CCGG 4 cut(s) 45, 82, 359, 413
HphI GGTGA 3 cut(s) 38, 268, 427
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 2 cut(s) 167, 340
Hpy188III TCNNGA 5 cut(s) 304, 331, 382, 400, 427
Hpy8I GTNNAC 1 cut(s) 61
HpyAV CCTTC 1 cut(s) 454
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 2 cut(s) 139, 279
HpyF3I CTNAG 2 cut(s) 252, 339
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 1 cut(s) 430
KflI GGGWCCC 1 cut(s) 328
Kzo9I GATC 5 cut(s) 54, 300, 355, 372, 396
LguI GCTCTTC 1 cut(s) 99
Lsp1109I GCAGC 1 cut(s) 49
MaeI CTAG 2 cut(s) 6, 110
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 2 cut(s) 256, 415
MalI GATC 5 cut(s) 56, 302, 357, 374, 398
MboI GATC 5 cut(s) 54, 300, 355, 372, 396
MboII GAAGA 7 cut(s) 41, 83, 116, 298, 301, 379, 382
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 1 cut(s) 18
MluI ACGCGT 1 cut(s) 233
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 8 cut(s) 140, 153, 155, 214, 247, 346, 427, 430
MspI CCGG 4 cut(s) 45, 82, 359, 413
MspR9I CCNGG 1 cut(s) 46
MvnI CGCG 1 cut(s) 235
NciI CCSGG 1 cut(s) 46
NdeII GATC 5 cut(s) 54, 300, 355, 372, 396
NlaIII CATG 1 cut(s) 430
NlaIV GGNNCC 5 cut(s) 67, 226, 329, 330, 357
NmuCI GTSAC 2 cut(s) 256, 415
PagI TCATGA 1 cut(s) 426
PciSI GCTCTTC 1 cut(s) 99
PkrI GCNGC 1 cut(s) 39
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Ppu21I YACGTR 1 cut(s) 268
PpuMI RGGWCCY 2 cut(s) 142, 328
Psp5II RGGWCCY 2 cut(s) 142, 328
PspN4I GGNNCC 5 cut(s) 67, 226, 329, 330, 357
PspPI GGNCC 4 cut(s) 65, 142, 225, 328
PspPPI RGGWCCY 2 cut(s) 142, 328
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 2 cut(s) 62, 266
RsaNI GTAC 2 cut(s) 61, 265
SapI GCTCTTC 1 cut(s) 99
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 5 cut(s) 54, 300, 355, 372, 396
Sau96I GGNCC 4 cut(s) 65, 142, 225, 328
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 46
SduI GDGCHC 1 cut(s) 213
SetI ASST 8 cut(s) 99, 111, 147, 245, 258, 270, 318, 465
SinI GGWCC 3 cut(s) 142, 225, 328
Sse9I AATT 1 cut(s) 18
SsiI CCGC 3 cut(s) 50, 156, 362
SspMI CTAG 2 cut(s) 6, 110
StyD4I CCNGG 1 cut(s) 44
TaiI ACGT 1 cut(s) 270
TasI AATT 1 cut(s) 18
TseFI GTSAC 2 cut(s) 256, 415
TseI GCWGC 1 cut(s) 37
Tsp45I GTSAC 2 cut(s) 256, 415
TspDTI ATGAA 3 cut(s) 203, 299, 415
TspGWI ACGGA 2 cut(s) 378, 446
VpaK11BI GGWCC 3 cut(s) 142, 225, 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XspI CTAG 2 cut(s) 6, 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.