RLG00000026968

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005672
Physical Location & Seq
Reverse (-)
7316502 .. 7320383
3882 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026968

Sequence Viewer

Length: 918 bp
ATGAAGCTGCAGGAAGCTTATAGCCGAAATGCCCCCAAAAATCTAAAAACCCCCAAAGCTAATTCTGGAAAATTGTTGTTTCCTTCACCGAATGCAAGTACTGTGAAGAAGGTTGTGTTTCCTCGTCGTCAATCCTTCAGATTTAATTCAGTGAAGCAAAACTTTTTCTTCTTAGCTAGGTTTGGGAGAAGCACAAGAAATATGGCTTTGACAAACTTCATACTGACAGTGGCTGGTGTAAGTGCTGTAGTTCTTTTATTGAGGAGTGATGTGAAACAGTCAGCTACCATATTCAGGCGGAACGTCAAACATATCCGCAAGTGGCTTGAAGAAGAATCCAAGGCAGTCCCGAAGGAACTGGAAACAAAGGTTCCGCCGAAGGATGTTCCCAAGGAGGACAAACACTATAAACTCTCCCCAGTTCTAGCTGCATGTAGTTATGTGATTGACCTCACCACAGAAATGGCTCTTGTGGAAGACAAGCGCGCAGGTGCTGAGATTGTGCATGGAGCTCAAGCGTGCTACCCACAGTCCATAGATCTCTTGGAAGAGCTGGGATTTCCCAAAGGTGTACTTCCCCTGCAAGACCTGGTGGAGTGTGGAAGGGTGAAGGAAACTGGGTTTGTGTGGATAAAGCAGAAGGCGCCATACGAGCACTTCTTCGCTGGGTCGAACACGCGTGTGAGCTACGCGACTGAGGTGACTGCGTATGTTGAGAAGGGCAGGATGAAGAAGATGACTGGGATCAAGAGCAAGCAGGTGTTTCTTTGGGTGCCTATTAGTGAGATGATCATGGAAGAGCCAGCAACCAAGAAGATCACTTTCAAGACTCCGATGGGGCTCGGAAGGACTTTTCCGGTCACGGCTTTCATGACCGAGGAGGAGAAGCAGAAGTATCTGGAGAATAGTAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

306

Amino Acids

34.59

Weight (kDa)

9.69

Isoelectric Point (pI)

59.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 180 - 290 1.7e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 479, 748
Acc36I ACCTGC 2 cut(s) 479, 748
AccB1I GGYRCC 2 cut(s) 643, 772
AccII CGCG 3 cut(s) 486, 679, 692
AciI CCGC 3 cut(s) 298, 316, 374
AclWI GGATC 1 cut(s) 752
AcuI CTGAAG 1 cut(s) 121
AcyI GRCGYC 1 cut(s) 644
AfaI GTAC 2 cut(s) 100, 573
AfiI CCNNNNNNNGG 1 cut(s) 294
AflIII ACRYGT 1 cut(s) 677
AgsI TTSAA 2 cut(s) 329, 826
AjnI CCWGG 1 cut(s) 588
AleI CACNNNNGTG 1 cut(s) 680
AluBI AGCT 9 cut(s) 7, 17, 59, 176, 284, 428, 512, 553, 687
AluI AGCT 9 cut(s) 7, 17, 59, 176, 284, 428, 512, 553, 687
Alw21I GWGCWC 2 cut(s) 514, 657
AlwI GGATC 1 cut(s) 752
AlwNI CAGNNNCTG 2 cut(s) 233, 494
ApeKI GCWGC 2 cut(s) 7, 428
AspLEI GCGC 3 cut(s) 486, 488, 646
AsuHPI GGTGA 4 cut(s) 78, 445, 619, 712
BanI GGYRCC 2 cut(s) 643, 772
BanII GRGCYC 2 cut(s) 514, 843
BarI GAAGNNNNNNTAC 2 cut(s) 632, 664
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 2 cut(s) 514, 657
BbvI GCAGC 1 cut(s) 415
BccI CCATC 1 cut(s) 829
BceAI ACGGC 1 cut(s) 879
BciT130I CCWGG 1 cut(s) 590
BclI TGATCA 1 cut(s) 789
BfaI CTAG 2 cut(s) 177, 425
BfmI CTRYAG 2 cut(s) 8, 246
BfoI RGCGCY 1 cut(s) 647
BfuAI ACCTGC 2 cut(s) 479, 748
BglII AGATCT 1 cut(s) 538
BisI GCNGC 2 cut(s) 8, 429
BlsI GCNGC 2 cut(s) 9, 430
BmcAI AGTACT 1 cut(s) 100
Bme1390I CCNGG 1 cut(s) 590
BmiI GGNNCC 3 cut(s) 372, 645, 774
BmrFI CCNGG 1 cut(s) 590
BmrI ACTGGG 3 cut(s) 413, 627, 750
BmuI ACTGGG 3 cut(s) 413, 627, 750
BpiI GAAGAC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 644
BsaJI CCNNGG 3 cut(s) 339, 390, 876
BsaWI WCCGGW 1 cut(s) 856
Bsc4I CCNNNNNNNGG 1 cut(s) 294
Bse1I ACTGG 4 cut(s) 363, 419, 622, 745
BseBI CCWGG 1 cut(s) 590
BseDI CCNNGG 3 cut(s) 339, 390, 876
BseGI GGATG 2 cut(s) 388, 732
BseLI CCNNNNNNNGG 1 cut(s) 294
BseMII CTCAG 2 cut(s) 486, 687
BseNI ACTGG 4 cut(s) 363, 419, 622, 745
BsePI GCGCGC 1 cut(s) 484
BseRI GAGGAG 3 cut(s) 277, 893, 896
BseXI GCAGC 1 cut(s) 415
BseYI CCCAGC 2 cut(s) 553, 665
Bsh1236I CGCG 3 cut(s) 486, 679, 692
BshNI GGYRCC 2 cut(s) 643, 772
BsiHKAI GWGCWC 2 cut(s) 514, 657
BsiSI CCGG 1 cut(s) 857
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 1 cut(s) 294
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 97
Bsp1286I GDGCHC 3 cut(s) 514, 657, 843
Bsp143I GATC 4 cut(s) 538, 744, 789, 816
BspACI CCGC 3 cut(s) 298, 316, 374
BspCNI CTCAG 2 cut(s) 487, 688
BspFNI CGCG 3 cut(s) 486, 679, 692
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 3 cut(s) 372, 645, 774
BspMAI CTGCAG 1 cut(s) 12
BspMI ACCTGC 2 cut(s) 479, 748
BspPI GGATC 1 cut(s) 752
BspQI GCTCTTC 2 cut(s) 543, 792
BspT107I GGYRCC 2 cut(s) 643, 772
BsrI ACTGG 4 cut(s) 363, 419, 622, 745
BssECI CCNNGG 3 cut(s) 339, 390, 876
BssHII GCGCGC 1 cut(s) 484
BssMI GATC 4 cut(s) 538, 744, 789, 816
BssNI GRCGYC 1 cut(s) 644
BssT1I CCWWGG 2 cut(s) 339, 390
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 4 cut(s) 103, 229, 279, 531
Bst6I CTCTTC 2 cut(s) 543, 792
BstACI GRCGYC 1 cut(s) 644
BstC8I GCNNGC 4 cut(s) 486, 520, 755, 804
BstDEI CTNAG 3 cut(s) 172, 495, 696
BstF5I GGATG 2 cut(s) 388, 732
BstFNI CGCG 3 cut(s) 486, 679, 692
BstH2I RGCGCY 1 cut(s) 647
BstHHI GCGC 3 cut(s) 486, 488, 646
BstKTI GATC 4 cut(s) 541, 747, 792, 819
BstMBI GATC 4 cut(s) 538, 744, 789, 816
BstMWI GCNNNNNNNGC 2 cut(s) 643, 652
BstNI CCWGG 1 cut(s) 590
BstNSI RCATGY 1 cut(s) 435
BstSCI CCNGG 1 cut(s) 588
BstSFI CTRYAG 2 cut(s) 8, 246
BstUI CGCG 3 cut(s) 486, 679, 692
BstV1I GCAGC 1 cut(s) 415
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 1 cut(s) 538
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 1 cut(s) 538
BtsCI GGATG 2 cut(s) 388, 732
BtsIMutI CAGTG 2 cut(s) 156, 234
BveI ACCTGC 2 cut(s) 479, 748
Cac8I GCNNGC 4 cut(s) 486, 520, 755, 804
CaiI CAGNNNCTG 2 cut(s) 233, 494
CciI TCATGA 1 cut(s) 870
CfoI GCGC 3 cut(s) 486, 488, 646
CsiI ACCWGGT 1 cut(s) 588
Csp6I GTAC 2 cut(s) 99, 572
CviAII CATG 4 cut(s) 432, 506, 793, 871
CviQI GTAC 2 cut(s) 99, 572
DdeI CTNAG 3 cut(s) 172, 495, 696
DinI GGCGCC 1 cut(s) 645
DpnI GATC 4 cut(s) 540, 746, 791, 818
DpnII GATC 4 cut(s) 538, 744, 789, 816
Eam1104I CTCTTC 2 cut(s) 543, 792
EarI CTCTTC 2 cut(s) 543, 792
EciI GGCGGA 2 cut(s) 313, 363
Ecl136II GAGCTC 1 cut(s) 512
Eco130I CCWWGG 2 cut(s) 339, 390
Eco24I GRGCYC 2 cut(s) 514, 843
Eco53kI GAGCTC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 588
EcoT14I CCWWGG 2 cut(s) 339, 390
EcoT38I GRGCYC 2 cut(s) 514, 843
EgeI GGCGCC 1 cut(s) 645
EheI GGCGCC 1 cut(s) 645
ErhI CCWWGG 2 cut(s) 339, 390
FaeI CATG 4 cut(s) 435, 509, 796, 874
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 558, 590
FaqI GGGAC 1 cut(s) 332
FatI CATG 4 cut(s) 431, 505, 792, 870
FbaI TGATCA 1 cut(s) 789
Fnu4HI GCNGC 2 cut(s) 8, 429
FokI GGATG 2 cut(s) 395, 739
FriOI GRGCYC 2 cut(s) 514, 843
Fsp4HI GCNGC 2 cut(s) 8, 429
FspBI CTAG 2 cut(s) 177, 425
GlaI GCGC 3 cut(s) 485, 487, 645
GluI GCNGC 2 cut(s) 8, 429
GsaI CCCAGC 2 cut(s) 557, 669
HaeII RGCGCY 1 cut(s) 647
HapII CCGG 1 cut(s) 857
HhaI GCGC 3 cut(s) 486, 488, 646
Hin1I GRCGYC 1 cut(s) 644
Hin1II CATG 4 cut(s) 435, 509, 796, 874
Hin6I GCGC 3 cut(s) 484, 486, 644
HinP1I GCGC 3 cut(s) 484, 486, 644
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 2 cut(s) 335, 829
HpaII CCGG 1 cut(s) 857
HphI GGTGA 4 cut(s) 78, 445, 619, 712
Hpy166II GTNNAC 1 cut(s) 572
Hpy188I TCNGA 3 cut(s) 140, 834, 845
Hpy188III TCNNGA 6 cut(s) 66, 349, 748, 826, 871, 899
Hpy8I GTNNAC 1 cut(s) 572
Hpy99I CGWCG 1 cut(s) 129
HpyCH4III ACNGT 4 cut(s) 103, 229, 279, 531
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 5 cut(s) 10, 95, 431, 505, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 643, 652
HpyF3I CTNAG 3 cut(s) 172, 495, 696
HpySE526I ACGT 1 cut(s) 303
Hsp92I GRCGYC 1 cut(s) 644
Hsp92II CATG 4 cut(s) 435, 509, 796, 874
HspAI GCGC 3 cut(s) 484, 486, 644
KasI GGCGCC 1 cut(s) 643
Ksp22I TGATCA 1 cut(s) 789
Kzo9I GATC 4 cut(s) 538, 744, 789, 816
LguI GCTCTTC 2 cut(s) 543, 792
LmnI GCTCC 1 cut(s) 509
Lsp1109I GCAGC 1 cut(s) 415
MabI ACCWGGT 1 cut(s) 588
MaeI CTAG 2 cut(s) 177, 425
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 700, 859
MalI GATC 4 cut(s) 540, 746, 791, 818
MboI GATC 4 cut(s) 538, 744, 789, 816
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 3 cut(s) 514, 657, 843
MluCI AATT 3 cut(s) 61, 71, 145
MluI ACGCGT 1 cut(s) 677
Mly113I GGCGCC 1 cut(s) 644
MlyI GAGTC 1 cut(s) 823
MnlI CCTC 7 cut(s) 132, 255, 388, 461, 691, 871, 874
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 2 cut(s) 461, 680
MspI CCGG 1 cut(s) 857
MspR9I CCNGG 1 cut(s) 590
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 1 cut(s) 590
MvnI CGCG 3 cut(s) 486, 679, 692
MwoI GCNNNNNNNGC 2 cut(s) 643, 652
NarI GGCGCC 1 cut(s) 644
NdeII GATC 4 cut(s) 538, 744, 789, 816
NlaIII CATG 4 cut(s) 435, 509, 796, 874
NlaIV GGNNCC 3 cut(s) 372, 645, 774
NmuCI GTSAC 2 cut(s) 700, 859
NspI RCATGY 1 cut(s) 435
OliI CACNNNNGTG 1 cut(s) 680
PagI TCATGA 1 cut(s) 870
PaqCI CACCTGC 2 cut(s) 479, 748
PauI GCGCGC 1 cut(s) 484
PciSI GCTCTTC 2 cut(s) 543, 792
PctI GAATGC 1 cut(s) 97
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 2 cut(s) 9, 430
PleI GAGTC 1 cut(s) 823
PluTI GGCGCC 1 cut(s) 647
PpsI GAGTC 1 cut(s) 823
Psp124BI GAGCTC 1 cut(s) 514
Psp6I CCWGG 1 cut(s) 588
PspFI CCCAGC 2 cut(s) 553, 665
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 3 cut(s) 372, 645, 774
PstI CTGCAG 1 cut(s) 12
PstNI CAGNNNCTG 2 cut(s) 233, 494
PsuI RGATCY 1 cut(s) 538
PteI GCGCGC 1 cut(s) 484
RsaI GTAC 2 cut(s) 100, 573
RsaNI GTAC 2 cut(s) 99, 572
RseI CAYNNNNRTG 2 cut(s) 461, 680
SacI GAGCTC 1 cut(s) 514
SapI GCTCTTC 2 cut(s) 543, 792
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 2 cut(s) 8, 429
Sau3AI GATC 4 cut(s) 538, 744, 789, 816
ScaI AGTACT 1 cut(s) 100
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 1 cut(s) 590
SduI GDGCHC 3 cut(s) 514, 657, 843
SexAI ACCWGGT 1 cut(s) 588
SfcI CTRYAG 2 cut(s) 8, 246
SfoI GGCGCC 1 cut(s) 645
SmiMI CAYNNNNRTG 2 cut(s) 461, 680
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
Sse9I AATT 3 cut(s) 61, 71, 145
SsiI CCGC 3 cut(s) 298, 316, 374
SspDI GGCGCC 1 cut(s) 643
SspMI CTAG 2 cut(s) 177, 425
SstI GAGCTC 1 cut(s) 514
StyD4I CCNGG 1 cut(s) 588
StyI CCWWGG 2 cut(s) 339, 390
TaaI ACNGT 4 cut(s) 103, 229, 279, 531
TaiI ACGT 1 cut(s) 306
TaqI TCGA 1 cut(s) 671
TaqII GACCGA 1 cut(s) 890
TasI AATT 3 cut(s) 61, 71, 145
TatI WGTACW 2 cut(s) 98, 571
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 2 cut(s) 156, 234
TseFI GTSAC 2 cut(s) 700, 859
TseI GCWGC 2 cut(s) 7, 428
Tsp45I GTSAC 2 cut(s) 700, 859
TspDTI ATGAA 4 cut(s) 17, 208, 743, 859
TspRI CASTG 2 cut(s) 156, 234
XceI RCATGY 1 cut(s) 435
XcmI CCANNNNNNNNNTGG 1 cut(s) 541
XspI CTAG 2 cut(s) 177, 425
ZrmI AGTACT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.