Rroxscaffold_3G00267740

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005672
Physical Location & Seq
Reverse (-)
60949303 .. 60953118
3816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267740.1

Sequence Viewer

Length: 654 bp
ATGGCCCAGCCCAACCTCACTGGATCCGAAAACGCATTTCGCCGAGGGTTCATCTTTACCCGATGCACGACGCCCACACCTGATTTTCTTGCTCCGGAAGCCACCACCACTCTTAACCGCCACCCACCTCTCTCAGCTACTCAACAACTCCCGGTTCACCGATTCTCAGTCAAAATTTCTCATCAGAACTCTACCACAGAAATGGCTCTTGTGGAAGACAAGCGTGCAGGTGCTGAGATTGTGCATGGAGCTCAAGAGTGCTACCAACAGTCCATAGATCTCTTGGAAGAGCTGGGATTTCCCAAAGGTGTTCTTCCCCTGCAAGACCTGGTGGAGTGTGGAAGGGTGAAGGAAACTGGGTTTGTGTGGATGAAGCAGAAGGCGCCATACGAGCACTTCTTTGTTGGGTCGAACACGCGTGTGAGCTACGCGACTGAGGTGACTGCGTACGTGGAGAAGGGCAGGATGAAGAAGATGACCGGGATCAAGAGCAAGCAGGTGTTTCTTTGGGTGCCTATTAGTGAGATGATCATGGAAGAGCCAGCAACCAAGAAGATCACTTTCAAGACTCCGATGGGGCTCGGAAGGACTTTTCCGGTCACGGCTTTCATGACCGAGGAGGAGAAGCAGAAGTATCTGGAGAACGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

217

Amino Acids

24.5

Weight (kDa)

8.37

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 93 - 203 1.4e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 218, 487
Acc36I ACCTGC 2 cut(s) 218, 487
AccB1I GGYRCC 2 cut(s) 382, 511
AccII CGCG 2 cut(s) 418, 431
AccIII TCCGGA 1 cut(s) 94
AciI CCGC 1 cut(s) 118
AclWI GGATC 3 cut(s) 18, 31, 491
AcsI RAATTY 1 cut(s) 174
AcyI GRCGYC 2 cut(s) 71, 383
AfaI GTAC 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 416
AgsI TTSAA 1 cut(s) 565
AjnI CCWGG 1 cut(s) 327
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 4 cut(s) 137, 251, 292, 426
AluI AGCT 4 cut(s) 137, 251, 292, 426
Alw21I GWGCWC 2 cut(s) 253, 396
AlwI GGATC 3 cut(s) 18, 31, 491
AlwNI CAGNNNCTG 1 cut(s) 233
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 174
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 2 cut(s) 152, 481
AsuHPI GGTGA 3 cut(s) 149, 358, 451
BamHI GGATCC 1 cut(s) 23
BanI GGYRCC 2 cut(s) 382, 511
BanII GRGCYC 2 cut(s) 253, 582
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 2 cut(s) 253, 396
BccI CCATC 1 cut(s) 568
BceAI ACGGC 1 cut(s) 618
BciT130I CCWGG 1 cut(s) 329
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 2 cut(s) 152, 481
BfoI RGCGCY 1 cut(s) 386
BfuAI ACCTGC 2 cut(s) 218, 487
BglII AGATCT 1 cut(s) 277
Bme1390I CCNGG 3 cut(s) 152, 329, 481
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 3 cut(s) 25, 384, 513
BmrFI CCNGG 3 cut(s) 152, 329, 481
BmrI ACTGGG 1 cut(s) 366
BmsI GCATC 1 cut(s) 53
BmuI ACTGGG 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 2 cut(s) 152, 481
BsaAI YACGTR 1 cut(s) 451
BsaHI GRCGYC 2 cut(s) 71, 383
BsaJI CCNNGG 2 cut(s) 43, 615
BsaWI WCCGGW 2 cut(s) 94, 595
Bse1I ACTGG 2 cut(s) 25, 361
BseAI TCCGGA 1 cut(s) 94
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 2 cut(s) 43, 615
BseGI GGATG 2 cut(s) 375, 471
BseMII CTCAG 4 cut(s) 147, 180, 225, 426
BseNI ACTGG 2 cut(s) 25, 361
BseRI GAGGAG 2 cut(s) 632, 635
BseYI CCCAGC 2 cut(s) 6, 292
BsgI GTGCAG 1 cut(s) 246
Bsh1236I CGCG 2 cut(s) 418, 431
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 382, 511
BsiHKAI GWGCWC 2 cut(s) 253, 396
BsiSI CCGG 4 cut(s) 95, 152, 480, 596
BsiWI CGTACG 1 cut(s) 447
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 3 cut(s) 253, 396, 582
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 5 cut(s) 23, 277, 483, 528, 555
BspACI CCGC 1 cut(s) 118
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 146, 179, 226, 427
BspEI TCCGGA 1 cut(s) 94
BspFNI CGCG 2 cut(s) 418, 431
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 3 cut(s) 25, 384, 513
BspMI ACCTGC 2 cut(s) 218, 487
BspPI GGATC 3 cut(s) 18, 31, 491
BspQI GCTCTTC 2 cut(s) 282, 531
BspT107I GGYRCC 2 cut(s) 382, 511
BsrI ACTGG 2 cut(s) 25, 361
BssECI CCNNGG 2 cut(s) 43, 615
BssMI GATC 5 cut(s) 23, 277, 483, 528, 555
BssNI GRCGYC 2 cut(s) 71, 383
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 2 cut(s) 270, 647
Bst6I CTCTTC 2 cut(s) 282, 531
BstACI GRCGYC 2 cut(s) 71, 383
BstBAI YACGTR 1 cut(s) 451
BstC8I GCNNGC 3 cut(s) 225, 494, 543
BstDEI CTNAG 4 cut(s) 133, 166, 234, 435
BstF5I GGATG 2 cut(s) 375, 471
BstFNI CGCG 2 cut(s) 418, 431
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 5 cut(s) 26, 280, 486, 531, 558
BstMBI GATC 5 cut(s) 23, 277, 483, 528, 555
BstMWI GCNNNNNNNGC 3 cut(s) 98, 382, 391
BstNI CCWGG 1 cut(s) 329
BstSCI CCNGG 3 cut(s) 150, 327, 479
BstUI CGCG 2 cut(s) 418, 431
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 2 cut(s) 23, 277
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 2 cut(s) 23, 277
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 375, 471
BtsIMutI CAGTG 1 cut(s) 18
BveI ACCTGC 2 cut(s) 218, 487
Cac8I GCNNGC 3 cut(s) 225, 494, 543
CaiI CAGNNNCTG 1 cut(s) 233
CciI TCATGA 1 cut(s) 609
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 79
CsiI ACCWGGT 1 cut(s) 327
Csp6I GTAC 1 cut(s) 448
CviAII CATG 3 cut(s) 245, 532, 610
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 4 cut(s) 133, 166, 234, 435
DinI GGCGCC 1 cut(s) 384
DpnI GATC 5 cut(s) 25, 279, 485, 530, 557
DpnII GATC 5 cut(s) 23, 277, 483, 528, 555
Eam1104I CTCTTC 2 cut(s) 282, 531
EarI CTCTTC 2 cut(s) 282, 531
Ecl136II GAGCTC 1 cut(s) 251
Eco24I GRGCYC 2 cut(s) 253, 582
Eco53kI GAGCTC 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 253, 582
EgeI GGCGCC 1 cut(s) 384
EheI GGCGCC 1 cut(s) 384
FaeI CATG 3 cut(s) 248, 535, 613
FaiI YATR 5 cut(s) 246, 275, 388, 533, 611
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FatI CATG 3 cut(s) 244, 531, 609
FbaI TGATCA 1 cut(s) 528
FokI GGATG 2 cut(s) 382, 478
FriOI GRGCYC 2 cut(s) 253, 582
GlaI GCGC 1 cut(s) 384
GsaI CCCAGC 2 cut(s) 10, 296
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 1 cut(s) 5
HapII CCGG 4 cut(s) 95, 152, 480, 596
HgaI GACGC 1 cut(s) 79
HhaI GCGC 1 cut(s) 385
Hin1I GRCGYC 2 cut(s) 71, 383
Hin1II CATG 3 cut(s) 248, 535, 613
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HinfI GANTC 2 cut(s) 162, 568
HpaII CCGG 4 cut(s) 95, 152, 480, 596
HphI GGTGA 3 cut(s) 149, 358, 451
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 4 cut(s) 28, 186, 573, 584
Hpy188III TCNNGA 6 cut(s) 95, 254, 487, 565, 610, 638
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 5 cut(s) 336, 343, 373, 451, 579
HpyCH4III ACNGT 2 cut(s) 270, 647
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 4 cut(s) 66, 227, 244, 322
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 382, 391
HpyF3I CTNAG 4 cut(s) 133, 166, 234, 435
HpySE526I ACGT 1 cut(s) 450
Hsp92I GRCGYC 2 cut(s) 71, 383
Hsp92II CATG 3 cut(s) 248, 535, 613
HspAI GCGC 1 cut(s) 383
KasI GGCGCC 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 5 cut(s) 23, 277, 483, 528, 555
LguI GCTCTTC 2 cut(s) 282, 531
LmnI GCTCC 2 cut(s) 97, 248
LweI GCATC 1 cut(s) 53
MabI ACCWGGT 1 cut(s) 327
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 2 cut(s) 439, 598
MalI GATC 5 cut(s) 25, 279, 485, 530, 557
MboI GATC 5 cut(s) 23, 277, 483, 528, 555
MboII GAAGA 7 cut(s) 227, 299, 305, 481, 484, 548, 565
MflI RGATCY 2 cut(s) 23, 277
MhlI GDGCHC 3 cut(s) 253, 396, 582
MluCI AATT 2 cut(s) 174, 649
MluI ACGCGT 1 cut(s) 416
Mly113I GGCGCC 1 cut(s) 383
MlyI GAGTC 1 cut(s) 562
MnlI CCTC 6 cut(s) 26, 38, 138, 430, 610, 613
MroI TCCGGA 1 cut(s) 94
MseI TTAA 1 cut(s) 114
MslI CAYNNNNRTG 2 cut(s) 200, 419
MspI CCGG 4 cut(s) 95, 152, 480, 596
MspR9I CCNGG 3 cut(s) 152, 329, 481
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 2 cut(s) 418, 431
MwoI GCNNNNNNNGC 3 cut(s) 98, 382, 391
NarI GGCGCC 1 cut(s) 383
NciI CCSGG 2 cut(s) 152, 481
NdeII GATC 5 cut(s) 23, 277, 483, 528, 555
NlaIII CATG 3 cut(s) 248, 535, 613
NlaIV GGNNCC 3 cut(s) 25, 384, 513
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 2 cut(s) 439, 598
OliI CACNNNNGTG 1 cut(s) 419
PagI TCATGA 1 cut(s) 609
PaqCI CACCTGC 2 cut(s) 218, 487
PciSI GCTCTTC 2 cut(s) 282, 531
PfeI GAWTC 1 cut(s) 162
Pfl23II CGTACG 1 cut(s) 447
PleI GAGTC 1 cut(s) 562
PluTI GGCGCC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 562
Ppu21I YACGTR 1 cut(s) 451
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 327
PspFI CCCAGC 2 cut(s) 6, 292
PspGI CCWGG 1 cut(s) 327
PspLI CGTACG 1 cut(s) 447
PspN4I GGNNCC 3 cut(s) 25, 384, 513
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 2 cut(s) 23, 277
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 2 cut(s) 200, 419
SacI GAGCTC 1 cut(s) 253
SapI GCTCTTC 2 cut(s) 282, 531
SaqAI TTAA 1 cut(s) 114
Sau3AI GATC 5 cut(s) 23, 277, 483, 528, 555
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 562
ScrFI CCNGG 3 cut(s) 152, 329, 481
SduI GDGCHC 3 cut(s) 253, 396, 582
SexAI ACCWGGT 1 cut(s) 327
SfaNI GCATC 1 cut(s) 53
SfoI GGCGCC 1 cut(s) 384
SmiMI CAYNNNNRTG 2 cut(s) 200, 419
SmlI CTYRAG 1 cut(s) 252
SmoI CTYRAG 1 cut(s) 252
Sse9I AATT 2 cut(s) 174, 649
SsiI CCGC 1 cut(s) 118
SspDI GGCGCC 1 cut(s) 382
SstI GAGCTC 1 cut(s) 253
StyD4I CCNGG 3 cut(s) 150, 327, 479
TaaI ACNGT 2 cut(s) 270, 647
TaiI ACGT 1 cut(s) 453
TaqI TCGA 1 cut(s) 410
TaqII GACCGA 1 cut(s) 629
TasI AATT 2 cut(s) 174, 649
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 2 cut(s) 439, 598
Tsp45I GTSAC 2 cut(s) 439, 598
TspDTI ATGAA 4 cut(s) 40, 386, 482, 598
TspRI CASTG 1 cut(s) 25
XapI RAATTY 1 cut(s) 174
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.