MD14G1126600.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005672
Physical Location & Seq
Forward (+)
20118402 .. 20118875
474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1126600.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGCTAGTGGCAAGCAACTTGTTGTTGAAGACAAGCAGCGTGCCGGGGCCGAGATCGTTTACGGCCCCGAAGATTGCTACCGGCAGTCCATAGACCTACTACATGAGCTAGGGTTTCCCAAAGGTGTCCTCCCCCTGCAGGACCTCGTGGAGTGCGGGAGGGTCAGAGAAACTGGGTTCGTGTGGATGAAGCAGAAGCAACCCTACGAGCACTTCTTTGAGGGGTCCAACACACGTGTCAGCTACGCAACTGAGGTGACTGGGTACGTGGAGAAGTGCAGGATGAAGAAGATGACTGGGATCAAGAGCAAGCAGGTTTTTCTGTGGGTCCCCATATCTGAGATGAGTGTTGAGGATCCGGCGGGGAAGAAGATCGTTTTCAAGACTCCGATGGGGATCGGGAAGTCTTTTCCGGTCACGGCGTTCATGACGGAGGAGGAGAAGCGGAAGTATTTGGAGAAGGTCAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

158

Amino Acids

17.95

Weight (kDa)

8.35

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 32 - 142 1e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 304
AciI CCGC 3 cut(s) 156, 362, 445
AclWI GGATC 4 cut(s) 308, 350, 363, 404
AcvI CACGTG 1 cut(s) 236
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 1 cut(s) 139
AflIII ACRYGT 2 cut(s) 233, 235
AgsI TTSAA 2 cut(s) 29, 382
AluBI AGCT 2 cut(s) 109, 243
AluI AGCT 2 cut(s) 109, 243
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 4 cut(s) 308, 350, 363, 404
AoxI GGCC 2 cut(s) 48, 64
ApeKI GCWGC 1 cut(s) 37
AspS9I GGNCC 5 cut(s) 48, 65, 142, 225, 328
AsuC2I CCSGG 1 cut(s) 46
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 3 cut(s) 142, 225, 328
BamHI GGATCC 1 cut(s) 355
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BauI CACGAG 1 cut(s) 146
BbrPI CACGTG 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 49
BccI CCATC 1 cut(s) 385
BceAI ACGGC 2 cut(s) 79, 435
BcnI CCSGG 1 cut(s) 46
BfaI CTAG 2 cut(s) 6, 110
BfmI CTRYAG 1 cut(s) 137
BfuAI ACCTGC 1 cut(s) 304
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 3 cut(s) 142, 225, 328
BmgT120I GGNCC 5 cut(s) 48, 65, 142, 225, 328
BmiI GGNNCC 6 cut(s) 49, 67, 226, 329, 330, 357
BmrFI CCNGG 1 cut(s) 46
BmrI ACTGGG 3 cut(s) 183, 270, 306
BmuI ACTGGG 3 cut(s) 183, 270, 306
BpiI GAAGAC 1 cut(s) 36
BpuMI CCSGG 1 cut(s) 46
BsaAI YACGTR 2 cut(s) 236, 268
BsaBI GATNNNNATC 1 cut(s) 395
BsaJI CCNNGG 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 412
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse118I RCCGGY 1 cut(s) 81
Bse1I ACTGG 3 cut(s) 178, 265, 301
Bse8I GATNNNNATC 1 cut(s) 395
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 2 cut(s) 192, 288
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMII CTCAG 2 cut(s) 243, 330
BseNI ACTGG 3 cut(s) 178, 265, 301
BseRI GAGGAG 2 cut(s) 449, 452
BseXI GCAGC 1 cut(s) 49
BsgI GTGCAG 1 cut(s) 298
BshFI GGCC 2 cut(s) 50, 66
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 4 cut(s) 45, 82, 359, 413
BslFI GGGAC 1 cut(s) 314
BslI CCNNNNNNNGG 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 314
BsnI GGCC 2 cut(s) 50, 66
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 5 cut(s) 54, 300, 355, 372, 396
BspACI CCGC 3 cut(s) 156, 362, 445
BspANI GGCC 2 cut(s) 50, 66
BspCNI CTCAG 2 cut(s) 244, 331
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 6 cut(s) 49, 67, 226, 329, 330, 357
BspMAI CTGCAG 1 cut(s) 141
BspMI ACCTGC 1 cut(s) 304
BspPI GGATC 4 cut(s) 308, 350, 363, 404
BsrFI RCCGGY 1 cut(s) 81
BsrI ACTGG 3 cut(s) 178, 265, 301
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 5 cut(s) 54, 300, 355, 372, 396
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
BstBAI YACGTR 2 cut(s) 236, 268
BstC8I GCNNGC 3 cut(s) 14, 42, 311
BstDEI CTNAG 2 cut(s) 252, 339
BstF5I GGATG 2 cut(s) 192, 288
BstKTI GATC 5 cut(s) 57, 303, 358, 375, 399
BstMBI GATC 5 cut(s) 54, 300, 355, 372, 396
BstSCI CCNGG 1 cut(s) 44
BstSFI CTRYAG 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 49
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 2 cut(s) 50, 66
BtsCI GGATG 2 cut(s) 192, 288
BveI ACCTGC 1 cut(s) 304
Cac8I GCNNGC 3 cut(s) 14, 42, 311
CciI TCATGA 1 cut(s) 426
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 5 cut(s) 48, 65, 142, 225, 328
Csp6I GTAC 1 cut(s) 265
CviAII CATG 2 cut(s) 104, 427
CviJI RGCY 5 cut(s) 5, 50, 66, 109, 243
CviKI_1 RGCY 5 cut(s) 5, 50, 66, 109, 243
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 2 cut(s) 252, 339
DpnI GATC 5 cut(s) 56, 302, 357, 374, 398
DpnII GATC 5 cut(s) 54, 300, 355, 372, 396
Eco47I GGWCC 3 cut(s) 142, 225, 328
Eco72I CACGTG 1 cut(s) 236
EcoO109I RGGNCCY 2 cut(s) 142, 328
FaeI CATG 2 cut(s) 107, 430
FaiI YATR 4 cut(s) 92, 105, 335, 428
FaqI GGGAC 1 cut(s) 314
FatI CATG 2 cut(s) 103, 426
FauI CCCGC 2 cut(s) 149, 355
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 2 cut(s) 199, 295
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 2 cut(s) 6, 110
GluI GCNGC 1 cut(s) 38
HaeIII GGCC 2 cut(s) 50, 66
HapII CCGG 4 cut(s) 45, 82, 359, 413
Hin1II CATG 2 cut(s) 107, 430
HinfI GANTC 1 cut(s) 385
HpaII CCGG 4 cut(s) 45, 82, 359, 413
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 3 cut(s) 167, 340, 390
Hpy188III TCNNGA 4 cut(s) 304, 382, 400, 427
Hpy8I GTNNAC 1 cut(s) 61
HpyAV CCTTC 1 cut(s) 454
HpyCH4IV ACGT 2 cut(s) 235, 267
HpyCH4V TGCA 2 cut(s) 139, 279
HpyF3I CTNAG 2 cut(s) 252, 339
HpySE526I ACGT 2 cut(s) 235, 267
Hsp92II CATG 2 cut(s) 107, 430
KflI GGGWCCC 1 cut(s) 328
Kzo9I GATC 5 cut(s) 54, 300, 355, 372, 396
Lsp1109I GCAGC 1 cut(s) 49
MaeI CTAG 2 cut(s) 6, 110
MaeII ACGT 2 cut(s) 235, 267
MaeIII GTNAC 2 cut(s) 256, 415
MalI GATC 5 cut(s) 56, 302, 357, 374, 398
MboI GATC 5 cut(s) 54, 300, 355, 372, 396
MboII GAAGA 6 cut(s) 41, 83, 298, 301, 379, 382
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 213
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 8 cut(s) 140, 153, 155, 214, 247, 346, 427, 430
MspI CCGG 4 cut(s) 45, 82, 359, 413
MspR9I CCNGG 1 cut(s) 46
NciI CCSGG 1 cut(s) 46
NdeII GATC 5 cut(s) 54, 300, 355, 372, 396
NlaIII CATG 2 cut(s) 107, 430
NlaIV GGNNCC 6 cut(s) 49, 67, 226, 329, 330, 357
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 256, 415
PagI TCATGA 1 cut(s) 426
PkrI GCNGC 1 cut(s) 39
PleI GAGTC 1 cut(s) 379
PmaCI CACGTG 1 cut(s) 236
PmlI CACGTG 1 cut(s) 236
PpsI GAGTC 1 cut(s) 379
Ppu21I YACGTR 2 cut(s) 236, 268
PpuMI RGGWCCY 2 cut(s) 142, 328
Psp5II RGGWCCY 2 cut(s) 142, 328
PspCI CACGTG 1 cut(s) 236
PspN4I GGNNCC 6 cut(s) 49, 67, 226, 329, 330, 357
PspPI GGNCC 5 cut(s) 48, 65, 142, 225, 328
PspPPI RGGWCCY 2 cut(s) 142, 328
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 5 cut(s) 54, 300, 355, 372, 396
Sau96I GGNCC 5 cut(s) 48, 65, 142, 225, 328
SbfI CCTGCAGG 1 cut(s) 141
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 46
SdaI CCTGCAGG 1 cut(s) 141
SduI GDGCHC 1 cut(s) 213
SfcI CTRYAG 1 cut(s) 137
SinI GGWCC 3 cut(s) 142, 225, 328
Sse8387I CCTGCAGG 1 cut(s) 141
SsiI CCGC 3 cut(s) 156, 362, 445
SspMI CTAG 2 cut(s) 6, 110
StyD4I CCNGG 1 cut(s) 44
TaiI ACGT 2 cut(s) 238, 270
TseFI GTSAC 2 cut(s) 256, 415
TseI GCWGC 1 cut(s) 37
Tsp45I GTSAC 2 cut(s) 256, 415
TspDTI ATGAA 3 cut(s) 203, 299, 415
TspGWI ACGGA 1 cut(s) 446
VpaK11BI GGWCC 3 cut(s) 142, 225, 328
XspI CTAG 2 cut(s) 6, 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.