Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005476

Physical Location & Seq

Genomic Coordinates Forward (+)

23860118 .. 23861053

Gene Structure 936 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1149600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 354 bp

ATGGCGTCACTGATACTCGGAGCTCCCCCAGCCCCCTCTCAGTCGCATGCTTTCAACTCCCGCATTTCGTTCTCTCGCTCCGAAACCCTTGCCATTTCTCTCCCCTCCTCCACTGCTTCGCTCTCGCTCTCCACAGCTTCTTCTCCGCCAGTTCCTTCAGTATACTGCGGCAGAGGCGATAAGAAAACCGAAAGGGGAAAGCGCTTCAATCACTCTTTTGGAAATGCGCGGCCGCGGAACAAGAACAAGGGCAGAGGACCACCTAGGGTTCCGGTGCCGCCGGCGCCCCCAAAGAAAGATAAGTTTGAGGACAATACGATTGTCAAAGTTGAAATTGATGAGTCCTTGTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009941 GO:0009987 GO:0010467 GO:0016043 GO:0019538 GO:0031967 GO:0031975 GO:0032544 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704 GO:0071840 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RPS31

Protein Analysis

118

Amino Acids

12.51

Weight (kDa)

10.34

Isoelectric Point (pI)

70.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPS31	PF17067	1 - 100	1.8e-39	Ribosomal protein S31e

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0005476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38140
fragaria_vesca	FvH4_6g11721 FvH4_6g11730
malus_domestica	MD04G1149600.v1.1 MD12G1164000.v1.1 MD12G1164100.v1.1
prunus_persica	Prupe.6G274500_v2.0.a1 Prupe.6G274500_v2.0.a1 Prupe.6G274600_v2.0.a1
pyrus_communis	pycom04g13510 pycom04g13520 pycom12g15680
rosa_chinensis	RchiOBHm_Chr3g0463501 RchiOBHm_Chr3g0463511
rosa_laevigata	RLG00000024782
rosa_multiflora	Rmu_sc0000923.1_g000021
rosa_roxburghii	Rroxscaffold_6G00416500 Rroxscaffold_6G00416510
rosa_rugosa	Rorug03G0065200 Rorug03G0065300
rosa_samantha	Rh3AG122400 Rh3AG122500 Rh3BG127100 Rh3BG127200 Rh3CG129000 Rh3CG129100 Rh3DG127900 Rh3DG128000
rosa_wichuraiana	Rw0G010590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	274, 283
AccI	GTMKAC	1 cut(s)	162
AccII	CGCG	2 cut(s)	229, 235
AciI	CCGC	7 cut(s)	61, 146, 168, 229, 233, 235, 278
AcoI	YGGCCR	1 cut(s)	230
AcuI	CTGAAG	1 cut(s)	141
AcyI	GRCGYC	2 cut(s)	5, 284
AfeI	AGCGCT	1 cut(s)	203
AgsI	TTSAA	3 cut(s)	55, 208, 332
AluBI	AGCT	2 cut(s)	23, 137
AluI	AGCT	2 cut(s)	23, 137
Alw21I	GWGCWC	1 cut(s)	25
Aor51HI	AGCGCT	1 cut(s)	203
AoxI	GGCC	1 cut(s)	230
AspA2I	CCTAGG	1 cut(s)	263
AspLEI	GCGC	3 cut(s)	204, 229, 286
AspS9I	GGNCC	1 cut(s)	257
AvaII	GGWCC	1 cut(s)	257
AvrII	CCTAGG	1 cut(s)	263
BanI	GGYRCC	2 cut(s)	274, 283
BanII	GRGCYC	1 cut(s)	25
Bbv12I	GWGCWC	1 cut(s)	25
BcgI	CGANNNNNNTGC	2 cut(s)	71, 105
BfaI	CTAG	1 cut(s)	264
BfoI	RGCGCY	2 cut(s)	205, 287
BisI	GCNGC	4 cut(s)	169, 230, 233, 278
BlnI	CCTAGG	1 cut(s)	263
BlsI	GCNGC	4 cut(s)	170, 231, 234, 279
Bme18I	GGWCC	1 cut(s)	257
BmgT120I	GGNCC	1 cut(s)	257
BmiI	GGNNCC	3 cut(s)	270, 276, 285
BsaHI	GRCGYC	2 cut(s)	5, 284
BsaJI	CCNNGG	2 cut(s)	233, 263
BsaWI	WCCGGW	1 cut(s)	271
Bse118I	RCCGGY	1 cut(s)	280
Bse1I	ACTGG	1 cut(s)	149
BseDI	CCNNGG	2 cut(s)	233, 263
BseMII	CTCAG	1 cut(s)	53
BseNI	ACTGG	1 cut(s)	149
BseRI	GAGGAG	1 cut(s)	97
BseX3I	CGGCCG	1 cut(s)	230
BseYI	CCCAGC	1 cut(s)	28
Bsh1236I	CGCG	2 cut(s)	229, 235
Bsh1285I	CGRYCG	1 cut(s)	233
BshFI	GGCC	1 cut(s)	232
BshNI	GGYRCC	2 cut(s)	274, 283
BsiEI	CGRYCG	1 cut(s)	233
BsiHKAI	GWGCWC	1 cut(s)	25
BsiSI	CCGG	2 cut(s)	272, 281
BsnI	GGCC	1 cut(s)	232
Bsp1286I	GDGCHC	1 cut(s)	25
BspACI	CCGC	7 cut(s)	61, 146, 168, 229, 233, 235, 278
BspANI	GGCC	1 cut(s)	232
BspCNI	CTCAG	1 cut(s)	52
BspFNI	CGCG	2 cut(s)	229, 235
BspLI	GGNNCC	3 cut(s)	270, 276, 285
BspT107I	GGYRCC	2 cut(s)	274, 283
BsrFI	RCCGGY	1 cut(s)	280
BsrI	ACTGG	1 cut(s)	149
BssAI	RCCGGY	1 cut(s)	280
BssECI	CCNNGG	2 cut(s)	233, 263
BssNAI	GTATAC	1 cut(s)	163
BssNI	GRCGYC	2 cut(s)	5, 284
BssT1I	CCWWGG	1 cut(s)	263
Bst1107I	GTATAC	1 cut(s)	163
BstACI	GRCGYC	2 cut(s)	5, 284
BstC8I	GCNNGC	2 cut(s)	48, 282
BstDEI	CTNAG	2 cut(s)	39, 351
BstDSI	CCRYGG	1 cut(s)	233
BstFNI	CGCG	2 cut(s)	229, 235
BstH2I	RGCGCY	2 cut(s)	205, 287
BstHHI	GCGC	3 cut(s)	204, 229, 286
BstMCI	CGRYCG	1 cut(s)	233
BstMWI	GCNNNNNNNGC	3 cut(s)	29, 174, 283
BstNSI	RCATGY	1 cut(s)	50
BstUI	CGCG	2 cut(s)	229, 235
BstZ17I	GTATAC	1 cut(s)	163
BstZI	CGGCCG	1 cut(s)	230
BsuRI	GGCC	1 cut(s)	232
BtgI	CCRYGG	1 cut(s)	233
BtsI	GCAGTG	1 cut(s)	111
BtsIMutI	CAGTG	2 cut(s)	8, 111
Cac8I	GCNNGC	2 cut(s)	48, 282
CciNI	GCGGCCGC	1 cut(s)	230
CfoI	GCGC	3 cut(s)	204, 229, 286
Cfr10I	RCCGGY	1 cut(s)	280
Cfr13I	GGNCC	1 cut(s)	257
Cfr42I	CCGCGG	1 cut(s)	236
CviAII	CATG	1 cut(s)	47
CviJI	RGCY	4 cut(s)	23, 32, 137, 232
CviKI_1	RGCY	4 cut(s)	23, 32, 137, 232
DdeI	CTNAG	2 cut(s)	39, 351
DinI	GGCGCC	1 cut(s)	285
EaeI	YGGCCR	1 cut(s)	230
EagI	CGGCCG	1 cut(s)	230
EciI	GGCGGA	1 cut(s)	135
Ecl136II	GAGCTC	1 cut(s)	23
EclXI	CGGCCG	1 cut(s)	230
Eco130I	CCWWGG	1 cut(s)	263
Eco24I	GRGCYC	1 cut(s)	25
Eco47I	GGWCC	1 cut(s)	257
Eco47III	AGCGCT	1 cut(s)	203
Eco52I	CGGCCG	1 cut(s)	230
Eco53kI	GAGCTC	1 cut(s)	23
Eco57I	CTGAAG	1 cut(s)	141
EcoICRI	GAGCTC	1 cut(s)	23
EcoT14I	CCWWGG	1 cut(s)	263
EcoT38I	GRGCYC	1 cut(s)	25
EgeI	GGCGCC	1 cut(s)	285
EheI	GGCGCC	1 cut(s)	285
ErhI	CCWWGG	1 cut(s)	263
FaeI	CATG	1 cut(s)	50
FaiI	YATR	2 cut(s)	48, 163
FatI	CATG	1 cut(s)	46
FauI	CCCGC	1 cut(s)	68
FblI	GTMKAC	1 cut(s)	162
Fnu4HI	GCNGC	4 cut(s)	169, 230, 233, 278
FriOI	GRGCYC	1 cut(s)	25
Fsp4HI	GCNGC	4 cut(s)	169, 230, 233, 278
FspBI	CTAG	1 cut(s)	264
GlaI	GCGC	3 cut(s)	203, 228, 285
GluI	GCNGC	4 cut(s)	169, 230, 233, 278
GsaI	CCCAGC	1 cut(s)	32
HaeII	RGCGCY	2 cut(s)	205, 287
HaeIII	GGCC	1 cut(s)	232
HapII	CCGG	2 cut(s)	272, 281
HhaI	GCGC	3 cut(s)	204, 229, 286
Hin1I	GRCGYC	2 cut(s)	5, 284
Hin1II	CATG	1 cut(s)	50
Hin6I	GCGC	3 cut(s)	202, 227, 284
HinP1I	GCGC	3 cut(s)	202, 227, 284
HinfI	GANTC	1 cut(s)	341
HpaII	CCGG	2 cut(s)	272, 281
Hpy166II	GTNNAC	1 cut(s)	163
Hpy188I	TCNGA	2 cut(s)	20, 82
Hpy8I	GTNNAC	1 cut(s)	163
HpyAV	CCTTC	1 cut(s)	165
HpyF10VI	GCNNNNNNNGC	3 cut(s)	29, 174, 283
HpyF3I	CTNAG	2 cut(s)	39, 351
Hsp92I	GRCGYC	2 cut(s)	5, 284
Hsp92II	CATG	1 cut(s)	50
HspAI	GCGC	3 cut(s)	202, 227, 284
KasI	GGCGCC	1 cut(s)	283
KroI	GCCGGC	1 cut(s)	280
KroNI	GCCGGC	1 cut(s)	282
KspI	CCGCGG	1 cut(s)	236
LmnI	GCTCC	3 cut(s)	20, 28, 83
LpnPI	CCDG	4 cut(s)	42, 162, 285, 294
MaeI	CTAG	1 cut(s)	264
MaeIII	GTNAC	1 cut(s)	6
MboII	GAAGA	1 cut(s)	132
MhlI	GDGCHC	1 cut(s)	25
MluCI	AATT	1 cut(s)	333
Mly113I	GGCGCC	1 cut(s)	284
MlyI	GAGTC	1 cut(s)	350
MnlI	CCTC	6 cut(s)	46, 115, 118, 167, 248, 301
MreI	CGCCGGCG	1 cut(s)	280
MroNI	GCCGGC	1 cut(s)	280
MspA1I	CMGCKG	1 cut(s)	235
MspI	CCGG	2 cut(s)	272, 281
MvnI	CGCG	2 cut(s)	229, 235
MwoI	GCNNNNNNNGC	3 cut(s)	29, 174, 283
NaeI	GCCGGC	1 cut(s)	282
NarI	GGCGCC	1 cut(s)	284
NgoMIV	GCCGGC	1 cut(s)	280
NlaIII	CATG	1 cut(s)	50
NlaIV	GGNNCC	3 cut(s)	270, 276, 285
NmuCI	GTSAC	1 cut(s)	6
NotI	GCGGCCGC	1 cut(s)	230
NspI	RCATGY	1 cut(s)	50
PaeI	GCATGC	1 cut(s)	50
PdiI	GCCGGC	1 cut(s)	282
PkrI	GCNGC	4 cut(s)	170, 231, 234, 279
PleI	GAGTC	1 cut(s)	349
PluTI	GGCGCC	1 cut(s)	287
PpsI	GAGTC	1 cut(s)	349
Psp124BI	GAGCTC	1 cut(s)	25
PspFI	CCCAGC	1 cut(s)	28
PspN4I	GGNNCC	3 cut(s)	270, 276, 285
PspPI	GGNCC	1 cut(s)	257
SacI	GAGCTC	1 cut(s)	25
SacII	CCGCGG	1 cut(s)	236
SatI	GCNGC	4 cut(s)	169, 230, 233, 278
Sau96I	GGNCC	1 cut(s)	257
SchI	GAGTC	1 cut(s)	350
SduI	GDGCHC	1 cut(s)	25
SetI	ASST	3 cut(s)	25, 139, 265
SfoI	GGCGCC	1 cut(s)	285
Sfr303I	CCGCGG	1 cut(s)	236
SgrAI	CRCCGGYG	1 cut(s)	280
SgrBI	CCGCGG	1 cut(s)	236
SinI	GGWCC	1 cut(s)	257
SphI	GCATGC	1 cut(s)	50
Sse9I	AATT	1 cut(s)	333
SsiI	CCGC	7 cut(s)	61, 146, 168, 229, 233, 235, 278
SspDI	GGCGCC	1 cut(s)	283
SspMI	CTAG	1 cut(s)	264
SstI	GAGCTC	1 cut(s)	25
StyI	CCWWGG	1 cut(s)	263
TasI	AATT	1 cut(s)	333
TauI	GCSGC	4 cut(s)	171, 232, 235, 280
TscAI	CASTG	2 cut(s)	15, 118
TseFI	GTSAC	1 cut(s)	6
Tsp45I	GTSAC	1 cut(s)	6
TspRI	CASTG	2 cut(s)	15, 118
VpaK11BI	GGWCC	1 cut(s)	257
XceI	RCATGY	1 cut(s)	50
XmaJI	CCTAGG	1 cut(s)	263
XmiI	GTMKAC	1 cut(s)	162
XspI	CTAG	1 cut(s)	264

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.