Rorug03G0065200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005476
Physical Location & Seq
Forward (+)
4831437 .. 4837440
6004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0065200.1

Sequence Viewer

Length: 2646 bp
ATGGAGATTCAAGCAGAAAACCCGCTGCCGGAGAAAGAGAGCAAACGGGTCGGGCACAGCGGCCCGCTAGTGACCAGCAGCAAGATGAGGAGCAACAGCAACAGAAGCGCAAGTTTCAAAGAAGACGATCAGTGCTATGTGGAAGTGAGTCAAGTGACACTGGATGTTTGCGACGACGATACCTTTGTGCTTCGGAACATCAGAGGAGCTGAACCTGAGTCCACGTCAGCAAGGCTAGAGAGAAGACCATCCCTTGCTTCTCAACTCTCTTTTAAACTCAAGAAAGTCTCGCATGAACTCAAGCGCATGAAATCTTCATTGAGCTTCAAGAAGGTCGACAGGCCCACCAAGCCCGGTGTTGCTCGTGCCCTTAAGGGACTCAAGCTCATGACCAAAAATGTGTGCAGTGAAGGTTGGCCAGAGATCCAAATAAGGTTTGATGAATTGGCTGTCGATGGAACTCTTCCAAAATCGGTCTTCGGTCAATGCATAGGGATGAAAGAGTCAAGTGAGTTTGCGGGCGAGGTGTTTGATGCATTGGCTCGTCGGAGAGGAATAACATCGTGTTGTGTGACCAAGGAAGAGTTGAGAGAATTTTGGGAACAAATTTCAGATCACAGCTTCAATGCCAAGCTTCAAACGTTCTTTGACATGGTAGATAAAAATGCAGATGGTCGAATCTCTGAAGAAGAGGTGAAAGAGATTATTACCTTAAGTGCCTCTGCTAATAAGCTGTCAAAAATTCTAGAACAAGCTGAGGAATATGCAGCATTGATTATGGAGGAGCTTGATCAAGACAACCTTGGATACATTGATTTGTACAACTTGGAAATCCTACTTCTTTCGGGCCCAACCCAATCTTCGAACCTGGAAACAGACAGCCGCATTCAGAGCCGGTTGCTAAGTGAAAAGCTGGTTCCGACCAAAGTGCACAATCCTATAAGGCGGTGGTGCCGGCGGCTGTCCTACTTTGTTGAGGATAATTGGAGGCGGATTTGGGTCATTGTGGTATGGCTTTTGATATGCATGGGCCTCTTTGCTTGGAAATTTATTCAATATAGACATAGGGAGGTCTTCCATGTAATGGGCTATTGTGTATGCATTGCCAAAGGTGGAGCTGAGACCCTAAAGTTTAACATGGCCTTGATTCTTCTACCAGTATGTAGAAATACAATTACTTGGCTAAGAAGCAAGACCAAGTTAGGAGGGGTAGTTCCATTTGATGACAATATCAATTTTCATAAGGTGATCGCGTTTGGAATTGCAGTTGGGGTCGGTCTCCATGCCGGTTCCCATCTTACATGCGACTTCCCCAAGCTACTCCACTCTACCAATGATCAATACGACAAGATGAAGCCATTTTTCGGCCAAAAACGACCCAAGGACTACTGGTGGTTTGTGAAGGGAACCGAGGGCTGGACTGGGGTGGTTATGGTGGCACTAATGCTAGTTGCATACACATTGGCTCAACCTTGGTTCCGTCGCAATCGACTGAGAAACCTTCCTAAAACACTAAAGAAACTTACTGGGTTCAATGCGTTTTGGTACTCCCATCACCTCTTTATCATTGTGTACATCCTCTTTATTATCCATGGATACTTTCTCTACCTCTCCAAGAACTGGGACAAGAAAACAACATGGATGTATCTGGCTTTCCCAGTAATAATGTATTCATGTGAGCGTTTGCTTCGGGCTTTCAGGTCTGGATATGAATCAGTGAAGATCTTAAAGGTCGCTGTTTACCCGGGAAACGTATTGGCACTCCATATGTCTAAACCACAGGGATTAAAATATACCAGTGGACAGTACATTTACATAAATTGTTCTGCTATTTCCCCATTTCAATGGCATCCATTCTCAATTACTTCAGCTCCTGGAGACGACTATCTAAGCGTTCATATTCGCACGTTGGGTGATTGGACATCTCAGCTCAAATACATTTTCTCTCAGGTTTGCCAACGTCCTCCTATAGAACACAGTGGCCTTCTAACAGCTGATATTGAACAATCCAATATCCAACCCAGGATGCCAAGACTTCTTATTGATGGTCCATATGGTGCACCAGCACAAGACTACAAAAACTACGAAGTTCTTCTACTTGTTGGACTTGGAATTGGAGCAACTCCATTAATAAGCATACTCAAAGATGTGCTTAACAACATTAAGCGACAAAAAGAACTAGAAGCTCAAATGTTGGTGGAAAATGGTGTTAAGAGGAAGTCTACTTTTGCAACAAAACGAGCTTATTTCTACTGGATCACAAGAGAGCAAGGCTCGTTTGAGTGGTTTAGGGGTGTGATGAATGAGGTCGCAGAAAATGACCTCGACGGCGTGATTGAGCTGCACAACTACTGCACCAGTGTTTATGAAGAAGGAGATGCTAGATCAGCTTTGATTACTATGCTTCAGTCTATAAACCATGCCAAGAATGGTGTGGATGTAGTTTCAGGAACGAGGGTGAAGACGCATTTTGCTAGACCGAATTGGCGTAATGTTTTTAAGCATGTGGCAGTTAAGCACCCTGATCAAAAAGTTGGAGTGTTTTATTGTGGTGCACATGGATTAGTTGGGGAGTTAAAGAGGTTAGCTCAAGACTTTTCGAGAAAAACTGAGACCAAGTTTGATTTCCATAAAGAGAACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

881

Amino Acids

101.02

Weight (kDa)

9.13

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 116 - 210 4e-33 Respiratory burst NADPH oxidase
EF-hand_7 PF13499 211 - 274 3.9e-06 EF-hand domain pair
Ferric_reduct PF01794 379 - 529 2.2e-11 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 572 - 689 1.4e-35 FAD-binding domain
NAD_binding_6 PF08030 695 - 863 3.3e-51 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 951
AccB7I CCANNNNNTGG 2 cut(s) 1084, 1620
AccI GTMKAC 2 cut(s) 336, 2222
AccII CGCG 1 cut(s) 1253
AciI CCGC 8 cut(s) 23, 60, 65, 518, 883, 946, 958, 991
AclI AACGTT 1 cut(s) 641
AclWI GGATC 2 cut(s) 418, 2264
AcoI YGGCCR 2 cut(s) 416, 1366
AcsI RAATTY 4 cut(s) 593, 606, 741, 1046
AcuI CTGAAG 3 cut(s) 705, 1851, 2390
AfaI GTAC 4 cut(s) 821, 1547, 1574, 1808
AfiI CCNNNNNNNGG 5 cut(s) 28, 1084, 1364, 1416, 1620
AflII CTTAAG 2 cut(s) 371, 712
AgsI TTSAA 9 cut(s) 11, 118, 328, 625, 638, 1055, 1534, 1844, 2003
AjiI CACGTC 1 cut(s) 225
AjnI CCWGG 3 cut(s) 867, 1873, 2021
AjuI GAANNNNNNNTTGG 4 cut(s) 844, 876, 2609, 2641
AloI GAACNNNNNNTCC 2 cut(s) 1197, 1229
Alw21I GWGCWC 3 cut(s) 933, 2062, 2557
Alw26I GTCTC 5 cut(s) 292, 1115, 1283, 1872, 2606
Alw44I GTGCAC 3 cut(s) 929, 2058, 2553
AlwI GGATC 2 cut(s) 418, 2264
AlwNI CAGNNNCTG 1 cut(s) 1874
Ama87I CYCGRG 1 cut(s) 1744
AoxI GGCC 8 cut(s) 61, 341, 416, 847, 1030, 1140, 1366, 1981
ApaI GGGCCC 1 cut(s) 851
ApaLI GTGCAC 3 cut(s) 929, 2058, 2553
ApeKI GCWGC 4 cut(s) 25, 78, 767, 2341
ApoI RAATTY 4 cut(s) 593, 606, 741, 1046
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 1 cut(s) 2129
Asp700I GAANNNNTTC 1 cut(s) 2091
AspLEI GCGC 2 cut(s) 110, 306
AspS9I GGNCC 6 cut(s) 62, 342, 847, 848, 1030, 2048
AsuC2I CCSGG 3 cut(s) 354, 1745, 1746
AsuHPI GGTGA 5 cut(s) 706, 1258, 1547, 1925, 2470
AsuII TTCGAA 1 cut(s) 863
AvaI CYCGRG 1 cut(s) 1744
AvaII GGWCC 1 cut(s) 2048
BaeGI GKGCMC 6 cut(s) 57, 370, 851, 933, 2062, 2557
BaeI ACNNNNGTAYC 2 cut(s) 171, 204
BalI TGGCCA 1 cut(s) 418
BanI GGYRCC 1 cut(s) 951
BanII GRGCYC 1 cut(s) 851
BarI GAAGNNNNNNTAC 2 cut(s) 2079, 2111
BauI CACGAG 1 cut(s) 363
BbsI GAAGAC 5 cut(s) 129, 250, 469, 1066, 2468
Bbv12I GWGCWC 3 cut(s) 933, 2062, 2557
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 4 cut(s) 12, 90, 779, 2328
BccI CCATC 6 cut(s) 256, 449, 665, 1302, 1560, 2039
BceAI ACGGC 1 cut(s) 2344
BcgI CGANNNNNNTGC 4 cut(s) 31, 65, 910, 944
BciT130I CCWGG 3 cut(s) 869, 1875, 2023
BciVI GTATCC 2 cut(s) 800, 1589
BclI TGATCA 3 cut(s) 790, 1336, 2524
BcnI CCSGG 3 cut(s) 354, 1745, 1746
BcoDI GTCTC 5 cut(s) 292, 1115, 1283, 1872, 2606
BfaI CTAG 7 cut(s) 68, 236, 746, 1448, 2180, 2382, 2475
BfmI CTRYAG 1 cut(s) 1968
BfrI CTTAAG 2 cut(s) 371, 712
BfuI GTATCC 2 cut(s) 800, 1589
BglII AGATCT 1 cut(s) 1722
BisI GCNGC 7 cut(s) 26, 61, 79, 768, 883, 959, 2342
BlsI GCNGC 7 cut(s) 27, 62, 80, 769, 884, 960, 2343
Bme1390I CCNGG 6 cut(s) 354, 869, 1745, 1746, 1875, 2023
Bme18I GGWCC 1 cut(s) 2048
BmeT110I CYCGRG 1 cut(s) 1744
BmgBI CACGTC 1 cut(s) 225
BmgT120I GGNCC 6 cut(s) 62, 342, 847, 848, 1030, 2048
BmiI GGNNCC 6 cut(s) 849, 918, 953, 1291, 1408, 1478
BmrFI CCNGG 6 cut(s) 354, 869, 1745, 1746, 1875, 2023
BmrI ACTGGG 4 cut(s) 1431, 1536, 1630, 1652
BmsI GCATC 4 cut(s) 523, 1858, 2016, 2368
BmuI ACTGGG 4 cut(s) 1431, 1536, 1630, 1652
BpiI GAAGAC 5 cut(s) 129, 250, 469, 1066, 2468
BplI GAGNNNNNCTC 4 cut(s) 2258, 2290, 2572, 2604
BpmI CTGGAG 1 cut(s) 1896
Bpu10I CCTNAGC 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 863
BpuEI CTTGAG 4 cut(s) 263, 284, 365, 2574
BpuMI CCSGG 3 cut(s) 354, 1745, 1746
BsaBI GATNNNNATC 1 cut(s) 1711
BsaI GGTCTC 3 cut(s) 1115, 1283, 2606
BsaJI CCNNGG 8 cut(s) 576, 802, 1380, 1410, 1472, 1591, 1744, 2021
BsaXI ACNNNNNCTCC 4 cut(s) 1197, 1227, 1855, 1885
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 1084, 1364, 1416, 1620
Bse118I RCCGGY 3 cut(s) 894, 954, 1286
Bse3DI GCAATG 1 cut(s) 1101
Bse8I GATNNNNATC 1 cut(s) 1711
BseBI CCWGG 3 cut(s) 869, 1875, 2023
BseDI CCNNGG 8 cut(s) 576, 802, 1380, 1410, 1472, 1591, 1744, 2021
BseGI GGATG 8 cut(s) 169, 248, 501, 1575, 1647, 1849, 2031, 2443
BseJI GATNNNNATC 1 cut(s) 1711
BseLI CCNNNNNNNGG 5 cut(s) 28, 1084, 1364, 1416, 1620
BseMI GCAATG 1 cut(s) 1101
BseMII CTCAG 7 cut(s) 207, 747, 1110, 1484, 1940, 1961, 2601
BseRI GAGGAG 3 cut(s) 103, 219, 797
BseSI GKGCMC 6 cut(s) 57, 370, 851, 933, 2062, 2557
BseXI GCAGC 4 cut(s) 12, 90, 779, 2328
BsgI GTGCAG 3 cut(s) 424, 2327, 2338
Bsh1236I CGCG 1 cut(s) 1253
BshFI GGCC 8 cut(s) 63, 343, 418, 849, 1032, 1142, 1368, 1983
BshNI GGYRCC 1 cut(s) 951
BsiHKAI GWGCWC 3 cut(s) 933, 2062, 2557
BsiHKCI CYCGRG 1 cut(s) 1744
BsiSI CCGG 6 cut(s) 29, 354, 895, 955, 1287, 1745
BslFI GGGAC 2 cut(s) 390, 1637
BslI CCNNNNNNNGG 5 cut(s) 28, 1084, 1364, 1416, 1620
BsmAI GTCTC 5 cut(s) 292, 1115, 1283, 1872, 2606
BsmBI CGTCTC 1 cut(s) 1872
BsmFI GGGAC 2 cut(s) 390, 1637
BsmI GAATGC 1 cut(s) 885
BsnI GGCC 8 cut(s) 63, 343, 418, 849, 1032, 1142, 1368, 1983
Bso31I GGTCTC 3 cut(s) 1115, 1283, 2606
BsoBI CYCGRG 1 cut(s) 1744
Bsp119I TTCGAA 1 cut(s) 863
Bsp120I GGGCCC 1 cut(s) 847
Bsp1286I GDGCHC 6 cut(s) 57, 370, 851, 933, 2062, 2557
Bsp1407I TGTACA 2 cut(s) 819, 1572
Bsp19I CCATGG 1 cut(s) 1591
BspACI CCGC 8 cut(s) 23, 60, 65, 518, 883, 946, 958, 991
BspANI GGCC 8 cut(s) 63, 343, 418, 849, 1032, 1142, 1368, 1983
BspCNI CTCAG 7 cut(s) 208, 748, 1111, 1485, 1939, 1960, 2602
BspFNI CGCG 1 cut(s) 1253
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 6 cut(s) 849, 918, 953, 1291, 1408, 1478
BspPI GGATC 2 cut(s) 418, 2264
BspT104I TTCGAA 1 cut(s) 863
BspT107I GGYRCC 1 cut(s) 951
BspTI CTTAAG 2 cut(s) 371, 712
BspTNI GGTCTC 3 cut(s) 1115, 1283, 2606
BsrDI GCAATG 1 cut(s) 1101
BsrFI RCCGGY 3 cut(s) 894, 954, 1286
BsrGI TGTACA 2 cut(s) 819, 1572
BssAI RCCGGY 3 cut(s) 894, 954, 1286
BssECI CCNNGG 8 cut(s) 576, 802, 1380, 1410, 1472, 1591, 1744, 2021
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 5 cut(s) 576, 802, 1380, 1472, 1591
Bst2BI CACGAG 1 cut(s) 363
Bst2UI CCWGG 3 cut(s) 869, 1875, 2023
Bst4CI ACNGT 2 cut(s) 1806, 1979
Bst6I CTCTTC 3 cut(s) 468, 576, 684
BstAFI CTTAAG 2 cut(s) 371, 712
BstAUI TGTACA 2 cut(s) 819, 1572
BstBI TTCGAA 1 cut(s) 863
BstC8I GCNNGC 3 cut(s) 65, 520, 956
BstDSI CCRYGG 1 cut(s) 1591
BstF5I GGATG 8 cut(s) 169, 248, 501, 1575, 1647, 1849, 2031, 2443
BstFNI CGCG 1 cut(s) 1253
BstHHI GCGC 2 cut(s) 110, 306
BstMAI GTCTC 5 cut(s) 292, 1115, 1283, 1872, 2606
BstMWI GCNNNNNNNGC 4 cut(s) 105, 349, 891, 2387
BstNI CCWGG 3 cut(s) 869, 1875, 2023
BstNSI RCATGY 2 cut(s) 1305, 2507
BstSCI CCNGG 6 cut(s) 352, 867, 1743, 1744, 1873, 2021
BstSFI CTRYAG 1 cut(s) 1968
BstSLI GKGCMC 6 cut(s) 57, 370, 851, 933, 2062, 2557
BstUI CGCG 1 cut(s) 1253
BstV1I GCAGC 4 cut(s) 12, 90, 779, 2328
BstV2I GAAGAC 5 cut(s) 129, 250, 469, 1066, 2468
BstX2I RGATCY 2 cut(s) 423, 1722
BstXI CCANNNNNNTGG 1 cut(s) 1845
BstYI RGATCY 2 cut(s) 423, 1722
BsuI GTATCC 2 cut(s) 800, 1589
BsuRI GGCC 8 cut(s) 63, 343, 418, 849, 1032, 1142, 1368, 1983
BtgI CCRYGG 1 cut(s) 1591
BtrI CACGTC 1 cut(s) 225
BtsCI GGATG 8 cut(s) 169, 248, 501, 1575, 1647, 1849, 2031, 2443
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 7 cut(s) 137, 158, 412, 1722, 1804, 1984, 2365
Cac8I GCNNGC 3 cut(s) 65, 520, 956
CaiI CAGNNNCTG 1 cut(s) 1874
CciI TCATGA 1 cut(s) 387
CfoI GCGC 2 cut(s) 110, 306
Cfr10I RCCGGY 3 cut(s) 894, 954, 1286
Cfr13I GGNCC 6 cut(s) 62, 342, 847, 848, 1030, 2048
Cfr9I CCCGGG 1 cut(s) 1744
CseI GACGC 1 cut(s) 2473
Csp6I GTAC 4 cut(s) 820, 1546, 1573, 1807
CviQI GTAC 4 cut(s) 820, 1546, 1573, 1807
DraI TTTAAA 1 cut(s) 274
EaeI YGGCCR 2 cut(s) 416, 1366
Eam1104I CTCTTC 3 cut(s) 468, 576, 684
EarI CTCTTC 3 cut(s) 468, 576, 684
EciI GGCGGA 1 cut(s) 1006
Eco130I CCWWGG 5 cut(s) 576, 802, 1380, 1472, 1591
Eco24I GRGCYC 1 cut(s) 851
Eco31I GGTCTC 3 cut(s) 1115, 1283, 2606
Eco47I GGWCC 1 cut(s) 2048
Eco57I CTGAAG 3 cut(s) 705, 1851, 2390
Eco88I CYCGRG 1 cut(s) 1744
EcoRII CCWGG 3 cut(s) 867, 1873, 2021
EcoT14I CCWWGG 5 cut(s) 576, 802, 1380, 1472, 1591
EcoT22I ATGCAT 4 cut(s) 491, 538, 1028, 1103
EcoT38I GRGCYC 1 cut(s) 851
ErhI CCWWGG 5 cut(s) 576, 802, 1380, 1472, 1591
Esp3I CGTCTC 1 cut(s) 1872
FalI AAGNNNNNCTT 4 cut(s) 786, 818, 2136, 2168
FaqI GGGAC 2 cut(s) 390, 1637
FauI CCCGC 3 cut(s) 30, 72, 511
FauNDI CATATG 2 cut(s) 1767, 2053
FbaI TGATCA 3 cut(s) 790, 1336, 2524
FblI GTMKAC 2 cut(s) 336, 2222
Fnu4HI GCNGC 7 cut(s) 26, 61, 79, 768, 883, 959, 2342
FokI GGATG 8 cut(s) 176, 235, 508, 1562, 1654, 1836, 2038, 2450
FriOI GRGCYC 1 cut(s) 851
Fsp4HI GCNGC 7 cut(s) 26, 61, 79, 768, 883, 959, 2342
FspBI CTAG 7 cut(s) 68, 236, 746, 1448, 2180, 2382, 2475
GlaI GCGC 2 cut(s) 109, 305
GluI GCNGC 7 cut(s) 26, 61, 79, 768, 883, 959, 2342
GsuI CTGGAG 1 cut(s) 1896
HaeIII GGCC 8 cut(s) 63, 343, 418, 849, 1032, 1142, 1368, 1983
HapII CCGG 6 cut(s) 29, 354, 895, 955, 1287, 1745
HgaI GACGC 1 cut(s) 2473
HhaI GCGC 2 cut(s) 110, 306
Hin6I GCGC 2 cut(s) 108, 304
HinP1I GCGC 2 cut(s) 108, 304
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 8 cut(s) 7, 148, 218, 378, 503, 678, 1147, 1712
HpaII CCGG 6 cut(s) 29, 354, 895, 955, 1287, 1745
HphI GGTGA 5 cut(s) 706, 1258, 1547, 1925, 2470
Hpy166II GTNNAC 9 cut(s) 222, 337, 931, 1573, 1741, 1802, 2060, 2223, 2555
Hpy188I TCNGA 7 cut(s) 195, 203, 549, 613, 685, 891, 921
Hpy188III TCNNGA 9 cut(s) 280, 328, 388, 746, 794, 1704, 2448, 2591, 2601
Hpy8I GTNNAC 9 cut(s) 222, 337, 931, 1573, 1741, 1802, 2060, 2223, 2555
Hpy99I CGWCG 5 cut(s) 176, 179, 549, 1485, 2330
HpyAV CCTTC 6 cut(s) 325, 404, 1396, 1511, 1994, 2366
HpyCH4III ACNGT 2 cut(s) 1806, 1979
HpyCH4IV ACGT 5 cut(s) 224, 641, 1752, 1907, 1960
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 349, 891, 2387
HpySE526I ACGT 5 cut(s) 224, 641, 1752, 1907, 1960
HspAI GCGC 2 cut(s) 108, 304
KroI GCCGGC 1 cut(s) 954
KroNI GCCGGC 1 cut(s) 956
Ksp22I TGATCA 3 cut(s) 790, 1336, 2524
LmnI GCTCC 6 cut(s) 90, 206, 784, 1115, 1876, 2117
Lsp1109I GCAGC 4 cut(s) 12, 90, 779, 2328
LweI GCATC 4 cut(s) 523, 1858, 2016, 2368
MaeI CTAG 7 cut(s) 68, 236, 746, 1448, 2180, 2382, 2475
MaeII ACGT 5 cut(s) 224, 641, 1752, 1907, 1960
MaeIII GTNAC 3 cut(s) 70, 154, 571
MflI RGATCY 2 cut(s) 423, 1722
MhlI GDGCHC 6 cut(s) 57, 370, 851, 933, 2062, 2557
MlsI TGGCCA 1 cut(s) 418
MluNI TGGCCA 1 cut(s) 418
MlyI GAGTC 4 cut(s) 157, 227, 372, 512
MmeI TCCRAC 5 cut(s) 527, 944, 2041, 2083, 2515
Mox20I TGGCCA 1 cut(s) 418
Mph1103I ATGCAT 4 cut(s) 491, 538, 1028, 1103
MroNI GCCGGC 1 cut(s) 954
MroXI GAANNNNTTC 1 cut(s) 2091
MscI TGGCCA 1 cut(s) 418
MslI CAYNNNNRTG 2 cut(s) 494, 1843
Msp20I TGGCCA 1 cut(s) 418
MspA1I CMGCKG 3 cut(s) 25, 60, 1994
MspCI CTTAAG 2 cut(s) 371, 712
MspI CCGG 6 cut(s) 29, 354, 895, 955, 1287, 1745
MspR9I CCNGG 6 cut(s) 354, 869, 1745, 1746, 1875, 2023
Mva1269I GAATGC 1 cut(s) 885
MvaI CCWGG 3 cut(s) 869, 1875, 2023
MvnI CGCG 1 cut(s) 1253
MwoI GCNNNNNNNGC 4 cut(s) 105, 349, 891, 2387
NaeI GCCGGC 1 cut(s) 956
NciI CCSGG 3 cut(s) 354, 1745, 1746
NcoI CCATGG 1 cut(s) 1591
NdeI CATATG 2 cut(s) 1767, 2053
NgoMIV GCCGGC 1 cut(s) 954
NlaIV GGNNCC 6 cut(s) 849, 918, 953, 1291, 1408, 1478
NmuCI GTSAC 3 cut(s) 70, 154, 571
NsiI ATGCAT 4 cut(s) 491, 538, 1028, 1103
NspI RCATGY 2 cut(s) 1305, 2507
NspV TTCGAA 1 cut(s) 863
PagI TCATGA 1 cut(s) 387
PctI GAATGC 1 cut(s) 885
PdiI GCCGGC 1 cut(s) 956
PdmI GAANNNNTTC 1 cut(s) 2091
PfeI GAWTC 4 cut(s) 7, 678, 1147, 1712
PflMI CCANNNNNTGG 2 cut(s) 1084, 1620
PfoI TCCNGGA 1 cut(s) 1873
PkrI GCNGC 7 cut(s) 27, 62, 80, 769, 884, 960, 2343
PleI GAGTC 4 cut(s) 156, 226, 372, 511
PpsI GAGTC 4 cut(s) 156, 226, 372, 511
PshBI ATTAAT 1 cut(s) 2129
Psp1406I AACGTT 1 cut(s) 641
Psp6I CCWGG 3 cut(s) 867, 1873, 2021
PspGI CCWGG 3 cut(s) 867, 1873, 2021
PspN4I GGNNCC 6 cut(s) 849, 918, 953, 1291, 1408, 1478
PspOMI GGGCCC 1 cut(s) 847
PspPI GGNCC 6 cut(s) 62, 342, 847, 848, 1030, 2048
PstNI CAGNNNCTG 1 cut(s) 1874
PsuI RGATCY 2 cut(s) 423, 1722
PvuII CAGCTG 1 cut(s) 1994
RsaI GTAC 4 cut(s) 821, 1547, 1574, 1808
RsaNI GTAC 4 cut(s) 820, 1546, 1573, 1807
RseI CAYNNNNRTG 2 cut(s) 494, 1843
SalI GTCGAC 1 cut(s) 335
SatI GCNGC 7 cut(s) 26, 61, 79, 768, 883, 959, 2342
Sau96I GGNCC 6 cut(s) 62, 342, 847, 848, 1030, 2048
SchI GAGTC 4 cut(s) 157, 227, 372, 512
ScrFI CCNGG 6 cut(s) 354, 869, 1745, 1746, 1875, 2023
SduI GDGCHC 6 cut(s) 57, 370, 851, 933, 2062, 2557
SfaNI GCATC 4 cut(s) 523, 1858, 2016, 2368
SfcI CTRYAG 1 cut(s) 1968
SfuI TTCGAA 1 cut(s) 863
SinI GGWCC 1 cut(s) 2048
SmaI CCCGGG 1 cut(s) 1746
SmiMI CAYNNNNRTG 2 cut(s) 494, 1843
SmlI CTYRAG 6 cut(s) 278, 299, 371, 380, 712, 2589
SmoI CTYRAG 6 cut(s) 278, 299, 371, 380, 712, 2589
SsiI CCGC 8 cut(s) 23, 60, 65, 518, 883, 946, 958, 991
SspMI CTAG 7 cut(s) 68, 236, 746, 1448, 2180, 2382, 2475
StyD4I CCNGG 6 cut(s) 352, 867, 1743, 1744, 1873, 2021
StyI CCWWGG 5 cut(s) 576, 802, 1380, 1472, 1591
TaaI ACNGT 2 cut(s) 1806, 1979
TaiI ACGT 5 cut(s) 227, 644, 1755, 1910, 1963
TaqI TCGA 7 cut(s) 336, 453, 676, 863, 1489, 2325, 2600
TaqII GACCGA 4 cut(s) 463, 470, 1265, 2494
TatI WGTACW 3 cut(s) 819, 1572, 1806
TauI GCSGC 3 cut(s) 63, 885, 961
TfiI GAWTC 4 cut(s) 7, 678, 1147, 1712
TscAI CASTG 7 cut(s) 137, 165, 412, 1722, 1804, 1984, 2365
TseFI GTSAC 3 cut(s) 70, 154, 571
TseI GCWGC 4 cut(s) 25, 78, 767, 2341
Tsp45I GTSAC 3 cut(s) 70, 154, 571
TspGWI ACGGA 1 cut(s) 1469
TspMI CCCGGG 1 cut(s) 1744
TspRI CASTG 7 cut(s) 137, 165, 412, 1722, 1804, 1984, 2365
Van91I CCANNNNNTGG 2 cut(s) 1084, 1620
Vha464I CTTAAG 2 cut(s) 371, 712
VneI GTGCAC 3 cut(s) 929, 2058, 2553
VpaK11BI GGWCC 1 cut(s) 2048
VspI ATTAAT 1 cut(s) 2129
XapI RAATTY 4 cut(s) 593, 606, 741, 1046
XbaI TCTAGA 1 cut(s) 745
XceI RCATGY 2 cut(s) 1305, 2507
XcmI CCANNNNNNNNNTGG 2 cut(s) 2426, 2431
XmaI CCCGGG 1 cut(s) 1744
XmiI GTMKAC 2 cut(s) 336, 2222
XmnI GAANNNNTTC 1 cut(s) 2091
XspI CTAG 7 cut(s) 68, 236, 746, 1448, 2180, 2382, 2475
Zsp2I ATGCAT 4 cut(s) 491, 538, 1028, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.