pycom12g15680

30S ribosomal protein S31

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005476
Physical Location & Seq
Forward (+)
18159710 .. 18160465
756 bp
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UTR
Exon/CDS
Intron
pycom12g15680.1

Sequence Viewer

Length: 501 bp
ATGAGGCCCAACTTAATCTTGGGGCTTCTCAAAGCAAAATACAAAACGCACACAAAACATAACCGATATTCAACCCGACGGTCACTAAAACAGAGCACACGCATTTCCTCGAAAACCTTATCCGCTTATTCTCCTCAAGCTTCATCCATGGCGTCACTGATACTCGGAGCTCCACCAGCCCCCTCTCAATCGCTCGCTTTCAGCTCCCGAATTTCGTTCTCTCACTCCGAAACCCTAGTCGTTTCTCTCCCCTCCTCCACTGCTTCGCTATCACTCTCCACAGCTTCTTCTCCACCAGTCCCTTCAGTGTACTGCGGCAGAGGCGATAAGAAAACCGAAAGGGGAAAGCGCTTCAATCACTCTTTTGGCAATGCGCGGCCGCGGAACAAGAACAAAGGCAGAGGACCACCTAGGGTTCCGGTGCCGGCGGCTCCCCAAAAGAAAGATAAGTTTGAGGACGATACCATTGTCAAGGTTGAAATTGATGAGTCCTTGTCTTAA

Protein Analysis

167

Amino Acids

18.05

Weight (kDa)

10.64

Isoelectric Point (pI)

65.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPS31 PF17067 50 - 149 1.8e-39 Ribosomal protein S31e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 421
AccII CGCG 2 cut(s) 376, 382
AciI CCGC 6 cut(s) 123, 315, 376, 380, 382, 428
AcoI YGGCCR 1 cut(s) 377
AcsI RAATTY 1 cut(s) 210
AcuI CTGAAG 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 1 cut(s) 311
AfeI AGCGCT 1 cut(s) 350
AgsI TTSAA 3 cut(s) 72, 355, 479
AluBI AGCT 4 cut(s) 140, 170, 204, 284
AluI AGCT 4 cut(s) 140, 170, 204, 284
Alw21I GWGCWC 2 cut(s) 98, 172
Aor51HI AGCGCT 1 cut(s) 350
AoxI GGCC 2 cut(s) 5, 377
ApoI RAATTY 1 cut(s) 210
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AspA2I CCTAGG 1 cut(s) 410
AspLEI GCGC 2 cut(s) 351, 376
AspS9I GGNCC 2 cut(s) 6, 404
AvaII GGWCC 1 cut(s) 404
AvrII CCTAGG 1 cut(s) 410
BaeI ACNNNNGTAYC 2 cut(s) 453, 486
BanI GGYRCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 172
Bbv12I GWGCWC 2 cut(s) 98, 172
BfaI CTAG 2 cut(s) 236, 411
BfoI RGCGCY 1 cut(s) 352
BisI GCNGC 4 cut(s) 316, 377, 380, 429
BlnI CCTAGG 1 cut(s) 410
BlsI GCNGC 4 cut(s) 317, 378, 381, 430
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 2 cut(s) 6, 404
BmiI GGNNCC 3 cut(s) 417, 423, 432
BpuEI CTTGAG 1 cut(s) 120
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 147, 380, 410
BsaWI WCCGGW 1 cut(s) 418
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 376
BseDI CCNNGG 3 cut(s) 147, 380, 410
BseGI GGATG 1 cut(s) 143
BseMI GCAATG 1 cut(s) 376
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 2 cut(s) 123, 244
BseX3I CGGCCG 1 cut(s) 377
Bsh1236I CGCG 2 cut(s) 376, 382
Bsh1285I CGRYCG 1 cut(s) 380
BshFI GGCC 2 cut(s) 7, 379
BshNI GGYRCC 1 cut(s) 421
BsiEI CGRYCG 1 cut(s) 380
BsiHKAI GWGCWC 2 cut(s) 98, 172
BsiSI CCGG 2 cut(s) 419, 425
BslFI GGGAC 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 2 cut(s) 7, 379
Bsp1286I GDGCHC 2 cut(s) 98, 172
Bsp19I CCATGG 1 cut(s) 147
BspACI CCGC 6 cut(s) 123, 315, 376, 380, 382, 428
BspANI GGCC 2 cut(s) 7, 379
BspFNI CGCG 2 cut(s) 376, 382
BspLI GGNNCC 3 cut(s) 417, 423, 432
BspT107I GGYRCC 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 376
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 1 cut(s) 296
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 3 cut(s) 147, 380, 410
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 147, 410
Bst4CI ACNGT 1 cut(s) 81
BstACI GRCGYC 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 195, 426
BstDSI CCRYGG 2 cut(s) 147, 380
BstF5I GGATG 1 cut(s) 143
BstFNI CGCG 2 cut(s) 376, 382
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 2 cut(s) 351, 376
BstMCI CGRYCG 1 cut(s) 380
BstMWI GCNNNNNNNGC 2 cut(s) 176, 321
BstUI CGCG 2 cut(s) 376, 382
BstZI CGGCCG 1 cut(s) 377
BsuRI GGCC 2 cut(s) 7, 379
BtgI CCRYGG 2 cut(s) 147, 380
BtsCI GGATG 1 cut(s) 143
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 3 cut(s) 155, 258, 312
Cac8I GCNNGC 2 cut(s) 195, 426
CciNI GCGGCCGC 1 cut(s) 377
CfoI GCGC 2 cut(s) 351, 376
Cfr10I RCCGGY 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 6, 404
Cfr42I CCGCGG 1 cut(s) 383
CseI GACGC 1 cut(s) 141
Csp6I GTAC 1 cut(s) 310
CviAII CATG 1 cut(s) 148
CviJI RGCY 9 cut(s) 7, 25, 140, 170, 179, 204, 284, 379, 431
CviKI_1 RGCY 9 cut(s) 7, 25, 140, 170, 179, 204, 284, 379, 431
CviQI GTAC 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 377
EagI CGGCCG 1 cut(s) 377
Ecl136II GAGCTC 1 cut(s) 170
EclXI CGGCCG 1 cut(s) 377
Eco130I CCWWGG 2 cut(s) 147, 410
Eco24I GRGCYC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 404
Eco47III AGCGCT 1 cut(s) 350
Eco52I CGGCCG 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 170
EcoT14I CCWWGG 2 cut(s) 147, 410
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 147, 410
FaeI CATG 1 cut(s) 151
FaiI YATR 2 cut(s) 60, 149
FaqI GGGAC 1 cut(s) 284
FatI CATG 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 316, 377, 380, 429
FokI GGATG 1 cut(s) 130
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 4 cut(s) 316, 377, 380, 429
FspBI CTAG 2 cut(s) 236, 411
GlaI GCGC 2 cut(s) 350, 375
GluI GCNGC 4 cut(s) 316, 377, 380, 429
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 2 cut(s) 7, 379
HapII CCGG 2 cut(s) 419, 425
HgaI GACGC 1 cut(s) 141
HhaI GCGC 2 cut(s) 351, 376
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 2 cut(s) 349, 374
HinP1I GCGC 2 cut(s) 349, 374
HindIII AAGCTT 1 cut(s) 138
HinfI GANTC 1 cut(s) 488
HpaII CCGG 2 cut(s) 419, 425
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 2 cut(s) 167, 229
Hpy188III TCNNGA 1 cut(s) 207
Hpy8I GTNNAC 1 cut(s) 310
Hpy99I CGWCG 1 cut(s) 81
HpyAV CCTTC 1 cut(s) 312
HpyCH4III ACNGT 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 321
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 2 cut(s) 349, 374
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
KspI CCGCGG 1 cut(s) 383
LmnI GCTCC 4 cut(s) 167, 175, 209, 436
LpnPI CCDG 4 cut(s) 189, 309, 432, 438
MaeI CTAG 2 cut(s) 236, 411
MaeIII GTNAC 2 cut(s) 81, 153
MboII GAAGA 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 98, 172
MluCI AATT 2 cut(s) 210, 480
MlyI GAGTC 1 cut(s) 497
MnlI CCTC 8 cut(s) 118, 144, 193, 262, 265, 314, 395, 448
MroNI GCCGGC 1 cut(s) 424
MseI TTAA 2 cut(s) 14, 499
MspA1I CMGCKG 1 cut(s) 382
MspI CCGG 2 cut(s) 419, 425
MvnI CGCG 2 cut(s) 376, 382
MwoI GCNNNNNNNGC 2 cut(s) 176, 321
NaeI GCCGGC 1 cut(s) 426
NcoI CCATGG 1 cut(s) 147
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 1 cut(s) 151
NlaIV GGNNCC 3 cut(s) 417, 423, 432
NmuCI GTSAC 2 cut(s) 81, 153
NotI GCGGCCGC 1 cut(s) 377
PdiI GCCGGC 1 cut(s) 426
PkrI GCNGC 4 cut(s) 317, 378, 381, 430
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
Psp124BI GAGCTC 1 cut(s) 172
PspN4I GGNNCC 3 cut(s) 417, 423, 432
PspPI GGNCC 2 cut(s) 6, 404
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
SacI GAGCTC 1 cut(s) 172
SacII CCGCGG 1 cut(s) 383
SaqAI TTAA 2 cut(s) 14, 499
SatI GCNGC 4 cut(s) 316, 377, 380, 429
Sau96I GGNCC 2 cut(s) 6, 404
SchI GAGTC 1 cut(s) 497
SduI GDGCHC 2 cut(s) 98, 172
SetI ASST 7 cut(s) 119, 142, 172, 206, 286, 412, 477
Sfr303I CCGCGG 1 cut(s) 383
SgrBI CCGCGG 1 cut(s) 383
SinI GGWCC 1 cut(s) 404
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
Sse9I AATT 2 cut(s) 210, 480
SsiI CCGC 6 cut(s) 123, 315, 376, 380, 382, 428
SspMI CTAG 2 cut(s) 236, 411
SstI GAGCTC 1 cut(s) 172
StyI CCWWGG 2 cut(s) 147, 410
TaaI ACNGT 1 cut(s) 81
TaqI TCGA 1 cut(s) 110
TasI AATT 2 cut(s) 210, 480
TatI WGTACW 1 cut(s) 309
TauI GCSGC 4 cut(s) 318, 379, 382, 431
Tru1I TTAA 2 cut(s) 14, 499
Tru9I TTAA 2 cut(s) 14, 499
TscAI CASTG 3 cut(s) 162, 265, 312
TseFI GTSAC 2 cut(s) 81, 153
Tsp45I GTSAC 2 cut(s) 81, 153
TspDTI ATGAA 1 cut(s) 132
TspRI CASTG 3 cut(s) 162, 265, 312
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 1 cut(s) 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XmaJI CCTAGG 1 cut(s) 410
XspI CTAG 2 cut(s) 236, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.