MD04G1149800.v1.1

Lactosylceramide 4-alpha-galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013878
Physical Location & Seq
Reverse (-)
23862150 .. 23863442
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1149800.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGACCATGACTCGAATCGACAGCCGCCGCCTTAGCCTTGCCAAATTTCCCATCTTCTGTACTCATTTAATTGTTGCAATCATCGTCCTCGTCTATGCCGATAGTATTATCTATGGTGACTCGGAACAGTCAGTGGCGTCAAAGAACGAGGCGATTTCTCCGTTGCTGCAGAGTTATCATCATAATTCTGCAGGCGGATTGATCAAGTCTGATGTAAGTCACTTAGTTCTGCGTTCCATGCAGGAGGAGACCATTGAAGTTGGTAAAGAAAACACAAGGCCTCTTGTTCCTCCTTTAAATGTCACAGAAGAAGAAAGAATTGCTTGGTTCCGAAGCATGCTGTCGGAATTTCACATTTTCAAGTCAGACAAGTCTGCAAAGCAATTCCATGGCCGTGTTCTGGAATTCTTTAACAAGGAGTGTGAGGGTCAGTTCTTTATGACATGGATTTCGCCGGCGAGGTCTTTTGGGAGCAGAGAGTTCTTGACAATGGAGAGTCTCTTCAAAGCCCACCCTCGCGGATGTTTGATGATTCTATCGAGAACTATGGACTCCAAGTGCGGTTACAGAATTCTGAAACCGCTGAAGGATAGCGGGTTCAAAGTTCATGCTGTGACACCTGACTTGTCATTTCTGTTGAAAAACACGCCGGCAGAATCCTGGTTTGCTGAGATGAAGAGAGGCAAGAGAGACCCTGGTGAGATTCCATTAGCCCAGAACCTGTCTAATTTGATAAGACTCGCAGTTCTGTATAAGTATGGAGGTGTTTATTTAGACACAGACTTCATCGTCTTGAAATCGTTCTTCGGATTGAGAAACTCGATCGGTGCACAAAGTGTGGATGTGGTTTCGAAGAACTGGACCAGATTAAACAATGCAGTTCTGATCTTTGATATGAATCATCCCCTTCTGTACAAATTCATGGAGGAATTTGCCCTCACTTTTGATGGGAATAAATGGGGGCATAATGGTCCCTACCTTGTTTCCAGAGTGGTCCTGAGGGTGCAAAACCGACCCGGTTATAACTTCACGATTCTACCCCCTCTCGCCTTTTACCCCGTCGATTGGAGTAGGATCGGAGGATTGTTTAAGAAGCCAGGAAGTCCGGCTCATTCTCGATGGATAAGAGAAAAGCTGCTTCAGCTTAACGGGGAGACTTACGGAGTACACCTATGGAACAAGCAAAGCAGCAGAGAAGTAGTTGAAGAAGGAAGCATTATGCAGAGATTGATCTCCGATCGCTGTATCATTTGCAACGACATATACAGTTCTTCAAGAATTTTAATCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

431

Amino Acids

49.19

Weight (kDa)

9.33

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 159 - 279 4.8e-23 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 297 - 420 1e-26 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38152
fragaria_vesca FvH4_6g11720
malus_domestica MD04G1149800.v1.1 MD12G1164200.v1.1
prunus_persica Prupe.6G274700_v2.0.a1
pyrus_communis pycom04g13530
rosa_chinensis RchiOBHm_Chr3g0463491
rosa_laevigata RLG00000019555 RLG00000024783
rosa_multiflora Rmu_sc0000923.1_g000020
rosa_roxburghii Rroxscaffold_6G00416520
rosa_rugosa Rorug03G0065100
rosa_samantha Rh3AG122300 Rh3BG127000 Rh3CG128900 Rh3DG127800
rosa_wichuraiana Rw0G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
AasI GACNNNNNNGTC 1 cut(s) 788
AccII CGCG 1 cut(s) 519
AciI CCGC 7 cut(s) 25, 28, 195, 519, 561, 581, 594
AclWI GGATC 1 cut(s) 1082
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 7 cut(s) 44, 347, 404, 570, 917, 929, 1278
AcuI CTGAAG 2 cut(s) 605, 1124
AcyI GRCGYC 1 cut(s) 137
AdeI CACNNNGTG 1 cut(s) 836
AfaI GTAC 3 cut(s) 61, 914, 1167
AfiI CCNNNNNNNGG 2 cut(s) 400, 1065
AgsI TTSAA 8 cut(s) 257, 361, 505, 601, 640, 796, 1205, 1275
AjnI CCWGG 3 cut(s) 659, 694, 1096
AloI GAACNNNNNNTCC 2 cut(s) 581, 613
AluBI AGCT 2 cut(s) 1135, 1144
AluI AGCT 2 cut(s) 1135, 1144
Alw21I GWGCWC 1 cut(s) 832
Alw26I GTCTC 4 cut(s) 242, 503, 684, 1148
Alw44I GTGCAC 1 cut(s) 828
AlwI GGATC 1 cut(s) 1082
AlwNI CAGNNNCTG 1 cut(s) 721
AoxI GGCC 2 cut(s) 278, 391
ApaLI GTGCAC 1 cut(s) 828
ApeKI GCWGC 3 cut(s) 166, 1135, 1188
ApoI RAATTY 7 cut(s) 44, 347, 404, 570, 917, 929, 1278
ArsI GACNNNNNNTTYG 2 cut(s) 433, 465
Asp700I GAANNNNTTC 1 cut(s) 800
AspS9I GGNCC 3 cut(s) 861, 971, 994
AsuC2I CCSGG 1 cut(s) 1017
AsuHPI GGTGA 2 cut(s) 128, 710
AsuII TTCGAA 1 cut(s) 851
AvaII GGWCC 3 cut(s) 861, 971, 994
AxyI CCTNAGG 1 cut(s) 998
BaeGI GKGCMC 1 cut(s) 832
Bbv12I GWGCWC 1 cut(s) 832
BbvI GCAGC 3 cut(s) 153, 1122, 1200
BccI CCATC 3 cut(s) 59, 941, 1113
BceAI ACGGC 1 cut(s) 378
BciT130I CCWGG 3 cut(s) 661, 696, 1098
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 1 cut(s) 1017
BcoDI GTCTC 4 cut(s) 242, 503, 684, 1148
BfmI CTRYAG 2 cut(s) 167, 189
BisI GCNGC 5 cut(s) 25, 28, 167, 1136, 1189
BlsI GCNGC 5 cut(s) 26, 29, 168, 1137, 1190
Bme1390I CCNGG 4 cut(s) 661, 696, 1017, 1098
Bme18I GGWCC 3 cut(s) 861, 971, 994
BmgT120I GGNCC 3 cut(s) 861, 971, 994
BmiI GGNNCC 2 cut(s) 329, 973
BmrFI CCNGG 4 cut(s) 661, 696, 1017, 1098
Bpu10I CCTNAGC 1 cut(s) 32
Bpu14I TTCGAA 1 cut(s) 851
BpuMI CCSGG 1 cut(s) 1017
BsaBI GATNNNNATC 1 cut(s) 897
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 242, 684
BsaJI CCNNGG 2 cut(s) 388, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 400, 1065
Bse118I RCCGGY 2 cut(s) 454, 649
Bse1I ACTGG 1 cut(s) 863
Bse21I CCTNAGG 1 cut(s) 998
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 3 cut(s) 661, 696, 1098
BseDI CCNNGG 2 cut(s) 388, 694
BseGI GGATG 3 cut(s) 527, 847, 901
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 2 cut(s) 400, 1065
BseMII CTCAG 2 cut(s) 660, 989
BseNI ACTGG 1 cut(s) 863
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 832
BseXI GCAGC 3 cut(s) 153, 1122, 1200
Bsh1236I CGCG 1 cut(s) 519
Bsh1285I CGRYCG 2 cut(s) 825, 1240
BshFI GGCC 2 cut(s) 280, 393
BsiEI CGRYCG 2 cut(s) 825, 1240
BsiHKAI GWGCWC 1 cut(s) 832
BsiSI CCGG 4 cut(s) 455, 650, 1017, 1106
BslFI GGGAC 1 cut(s) 957
BslI CCNNNNNNNGG 2 cut(s) 400, 1065
BsmAI GTCTC 4 cut(s) 242, 503, 684, 1148
BsmFI GGGAC 1 cut(s) 957
BsnI GGCC 2 cut(s) 280, 393
Bso31I GGTCTC 2 cut(s) 242, 684
Bsp119I TTCGAA 1 cut(s) 851
Bsp1286I GDGCHC 1 cut(s) 832
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 6 cut(s) 201, 822, 885, 1074, 1230, 1237
Bsp19I CCATGG 1 cut(s) 388
BspACI CCGC 7 cut(s) 25, 28, 195, 519, 561, 581, 594
BspANI GGCC 2 cut(s) 280, 393
BspCNI CTCAG 2 cut(s) 661, 990
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 2 cut(s) 329, 973
BspMAI CTGCAG 2 cut(s) 171, 193
BspPI GGATC 1 cut(s) 1082
BspT104I TTCGAA 1 cut(s) 851
BspTNI GGTCTC 2 cut(s) 242, 684
BsrFI RCCGGY 2 cut(s) 454, 649
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 1 cut(s) 863
BssAI RCCGGY 2 cut(s) 454, 649
BssECI CCNNGG 2 cut(s) 388, 694
BssMI GATC 6 cut(s) 201, 822, 885, 1074, 1230, 1237
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 3 cut(s) 661, 696, 1098
Bst4CI ACNGT 2 cut(s) 129, 1268
Bst6I CTCTTC 2 cut(s) 506, 671
BstACI GRCGYC 1 cut(s) 137
BstAUI TGTACA 1 cut(s) 912
BstBI TTCGAA 1 cut(s) 851
BstC8I GCNNGC 4 cut(s) 193, 338, 456, 651
BstDEI CTNAG 4 cut(s) 32, 223, 669, 998
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 3 cut(s) 527, 847, 901
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 6 cut(s) 204, 825, 888, 1077, 1233, 1240
BstMAI GTCTC 4 cut(s) 242, 503, 684, 1148
BstMBI GATC 6 cut(s) 201, 822, 885, 1074, 1230, 1237
BstMCI CGRYCG 2 cut(s) 825, 1240
BstMWI GCNNNNNNNGC 3 cut(s) 33, 238, 1141
BstNI CCWGG 3 cut(s) 661, 696, 1098
BstNSI RCATGY 1 cut(s) 340
BstSCI CCNGG 4 cut(s) 659, 694, 1015, 1096
BstSFI CTRYAG 2 cut(s) 167, 189
BstSLI GKGCMC 1 cut(s) 832
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 3 cut(s) 153, 1122, 1200
Bsu36I CCTNAGG 1 cut(s) 998
BsuRI GGCC 2 cut(s) 280, 393
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 3 cut(s) 527, 847, 901
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 4 cut(s) 193, 338, 456, 651
CaiI CAGNNNCTG 1 cut(s) 721
Cfr10I RCCGGY 2 cut(s) 454, 649
Cfr13I GGNCC 3 cut(s) 861, 971, 994
CseI GACGC 1 cut(s) 126
Csp6I GTAC 3 cut(s) 60, 913, 1166
CviAII CATG 7 cut(s) 7, 238, 337, 389, 444, 608, 922
CviQI GTAC 3 cut(s) 60, 913, 1166
DdeI CTNAG 4 cut(s) 32, 223, 669, 998
DpnI GATC 6 cut(s) 203, 824, 887, 1076, 1232, 1239
DpnII GATC 6 cut(s) 201, 822, 885, 1074, 1230, 1237
DraI TTTAAA 1 cut(s) 297
DraIII CACNNNGTG 1 cut(s) 836
DrdI GACNNNNNNGTC 1 cut(s) 788
DseDI GACNNNNNNGTC 1 cut(s) 788
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 2 cut(s) 506, 671
EarI CTCTTC 2 cut(s) 506, 671
EciI GGCGGA 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 388
Eco147I AGGCCT 1 cut(s) 280
Eco31I GGTCTC 2 cut(s) 242, 684
Eco47I GGWCC 3 cut(s) 861, 971, 994
Eco57I CTGAAG 2 cut(s) 605, 1124
Eco81I CCTNAGG 1 cut(s) 998
EcoRI GAATTC 2 cut(s) 404, 570
EcoRII CCWGG 3 cut(s) 659, 694, 1096
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 7 cut(s) 10, 241, 340, 392, 447, 611, 925
FalI AAGNNNNNCTT 2 cut(s) 307, 339
FaqI GGGAC 1 cut(s) 957
FatI CATG 7 cut(s) 6, 237, 336, 388, 443, 607, 921
FauI CCCGC 1 cut(s) 587
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 5 cut(s) 25, 28, 167, 1136, 1189
FokI GGATG 3 cut(s) 534, 854, 888
Fsp4HI GCNGC 5 cut(s) 25, 28, 167, 1136, 1189
GluI GCNGC 5 cut(s) 25, 28, 167, 1136, 1189
HaeIII GGCC 2 cut(s) 280, 393
HapII CCGG 4 cut(s) 455, 650, 1017, 1106
HgaI GACGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 7 cut(s) 10, 241, 340, 392, 447, 611, 925
HpaII CCGG 4 cut(s) 455, 650, 1017, 1106
HphI GGTGA 2 cut(s) 128, 710
Hpy166II GTNNAC 2 cut(s) 830, 1168
Hpy188III TCNNGA 9 cut(s) 401, 484, 540, 793, 987, 997, 1030, 1116, 1275
Hpy8I GTNNAC 2 cut(s) 830, 1168
Hpy99I CGWCG 1 cut(s) 1064
HpyAV CCTTC 3 cut(s) 580, 917, 1202
HpyCH4III ACNGT 2 cut(s) 129, 1268
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 238, 1141
HpyF3I CTNAG 4 cut(s) 32, 223, 669, 998
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 7 cut(s) 10, 241, 340, 392, 447, 611, 925
KroI GCCGGC 2 cut(s) 454, 649
KroNI GCCGGC 2 cut(s) 456, 651
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 6 cut(s) 201, 822, 885, 1074, 1230, 1237
LmnI GCTCC 1 cut(s) 471
Lsp1109I GCAGC 3 cut(s) 153, 1122, 1200
MaeIII GTNAC 5 cut(s) 116, 218, 301, 563, 613
MalI GATC 6 cut(s) 203, 824, 887, 1076, 1232, 1239
MboI GATC 6 cut(s) 201, 822, 885, 1074, 1230, 1237
MboII GAAGA 9 cut(s) 46, 320, 323, 493, 688, 796, 865, 1217, 1263
MhlI GDGCHC 1 cut(s) 832
MlyI GAGTC 5 cut(s) 4, 113, 505, 545, 732
MmeI TCCRAC 1 cut(s) 324
MreI CGCCGGCG 1 cut(s) 454
MroNI GCCGGC 2 cut(s) 454, 649
MroXI GAANNNNTTC 1 cut(s) 800
MseI TTAA 7 cut(s) 68, 296, 411, 869, 1089, 1146, 1283
MslI CAYNNNNRTG 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 583
MspI CCGG 4 cut(s) 455, 650, 1017, 1106
MspR9I CCNGG 4 cut(s) 661, 696, 1017, 1098
MvaI CCWGG 3 cut(s) 661, 696, 1098
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 3 cut(s) 33, 238, 1141
NaeI GCCGGC 2 cut(s) 456, 651
NciI CCSGG 1 cut(s) 1017
NcoI CCATGG 1 cut(s) 388
NdeII GATC 6 cut(s) 201, 822, 885, 1074, 1230, 1237
NgoMIV GCCGGC 2 cut(s) 454, 649
NlaIII CATG 7 cut(s) 10, 241, 340, 392, 447, 611, 925
NlaIV GGNNCC 2 cut(s) 329, 973
NmuCI GTSAC 4 cut(s) 116, 218, 301, 613
NspI RCATGY 1 cut(s) 340
NspV TTCGAA 1 cut(s) 851
PaeI GCATGC 1 cut(s) 340
PceI AGGCCT 1 cut(s) 280
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 2 cut(s) 456, 651
PdmI GAANNNNTTC 1 cut(s) 800
PfeI GAWTC 6 cut(s) 15, 532, 656, 703, 898, 1033
PkrI GCNGC 5 cut(s) 26, 29, 168, 1137, 1190
Ple19I CGATCG 2 cut(s) 825, 1240
PleI GAGTC 5 cut(s) 4, 113, 504, 545, 732
PpsI GAGTC 5 cut(s) 4, 113, 504, 545, 732
PsiI TTATAA 1 cut(s) 1023
Psp6I CCWGG 3 cut(s) 659, 694, 1096
PspGI CCWGG 3 cut(s) 659, 694, 1096
PspN4I GGNNCC 2 cut(s) 329, 973
PspPI GGNCC 3 cut(s) 861, 971, 994
PstI CTGCAG 2 cut(s) 171, 193
PstNI CAGNNNCTG 1 cut(s) 721
PvuI CGATCG 2 cut(s) 825, 1240
RsaI GTAC 3 cut(s) 61, 914, 1167
RsaNI GTAC 3 cut(s) 60, 913, 1166
RseI CAYNNNNRTG 1 cut(s) 393
SaqAI TTAA 7 cut(s) 68, 296, 411, 869, 1089, 1146, 1283
SatI GCNGC 5 cut(s) 25, 28, 167, 1136, 1189
Sau3AI GATC 6 cut(s) 201, 822, 885, 1074, 1230, 1237
Sau96I GGNCC 3 cut(s) 861, 971, 994
SchI GAGTC 5 cut(s) 4, 113, 505, 545, 732
ScrFI CCNGG 4 cut(s) 661, 696, 1017, 1098
SduI GDGCHC 1 cut(s) 832
SetI ASST 8 cut(s) 464, 622, 723, 766, 981, 1137, 1146, 1173
SfcI CTRYAG 2 cut(s) 167, 189
SfuI TTCGAA 1 cut(s) 851
SgrAI CRCCGGYG 1 cut(s) 454
SinI GGWCC 3 cut(s) 861, 971, 994
SmiMI CAYNNNNRTG 1 cut(s) 393
SphI GCATGC 1 cut(s) 340
SseBI AGGCCT 1 cut(s) 280
SsiI CCGC 7 cut(s) 25, 28, 195, 519, 561, 581, 594
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 4 cut(s) 659, 694, 1015, 1096
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 129, 1268
TaqI TCGA 7 cut(s) 13, 18, 539, 821, 851, 1062, 1117
TatI WGTACW 3 cut(s) 59, 912, 1165
TauI GCSGC 2 cut(s) 27, 30
TfiI GAWTC 6 cut(s) 15, 532, 656, 703, 898, 1033
Tru1I TTAA 7 cut(s) 68, 296, 411, 869, 1089, 1146, 1283
Tru9I TTAA 7 cut(s) 68, 296, 411, 869, 1089, 1146, 1283
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 4 cut(s) 116, 218, 301, 613
TseI GCWGC 3 cut(s) 166, 1135, 1188
Tsp45I GTSAC 4 cut(s) 116, 218, 301, 613
TspDTI ATGAA 5 cut(s) 596, 689, 775, 910, 911
TspGWI ACGGA 2 cut(s) 150, 1176
TspRI CASTG 1 cut(s) 138
VneI GTGCAC 1 cut(s) 828
VpaK11BI GGWCC 3 cut(s) 861, 971, 994
XapI RAATTY 7 cut(s) 44, 347, 404, 570, 917, 929, 1278
XceI RCATGY 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.