RLG00000019555

Lactosylceramide 4-alpha-galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013878
Physical Location & Seq
Forward (+)
53505459 .. 53506671
1213 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019555

Sequence Viewer

Length: 954 bp
ATGAGTAGCCGAATCGACAGCCGCCTACTTAGCCTGGCGAAGTTCCACATCTTCTGCACTGTATCACTTGTTGCGATCATCTTCATCATCTATGCAGATGGTATCATATATACTGATTCCCAAAACTCAGTTGAGTTGAAAGATAAAGCTGTTTCTCAGGTGCTGCAGACTCATAGTATCGAACGCCAGTTCATCAAGTCTGATATAACTTGTAAAGCGCTGCGTTCTATGCAAGAAAATGTCCTTGATGTCGTTAAAGTTGACGGGAAGGCGCTGCAGTTTCATGGTAGAGTTCTTGAATTTTTTTACCATAAGTGTGAGACTCAGTTCTTCATGACATGGATATCGCCTGCGAGGTCTTTTGGAAGCAGAGAATTCCTGTCGATGGAGAGTGATCGCGGGTTCAAAGTTCATGCTGTGACGCCAGACTTGTCATTTCTGTTCAAGAACACCACAGCTGAGGCCTGGTTTGCTGAGATGAAGACAGGCAACAGAGACCCTGGTGAGATTCCATTAGCTCAGAACCTGTCTAATTTATTGAGACTAGCAGTTTTATACAAGTATGGAGGTGTGTACCTGGACACGGATTTCATTGTCGTGAAATCATTTTCGGGGTTGAGTAACTCAATCAGTGCACAAAGTACGGATGCGGTGTCGAAAAACTGGACCAGACTGAACAATGCAGTGCTGGTGTTTGATATGAATCATCCTCTTCTGCGCAAATTCATCGAGGAAGTGGTCCAAAGAGTGCAAAACAGACCTGGATATAACTTTACAGTCCTGCCTCCTATGGCTTTCTATCCAGTGGATTGGACTAGAATTGGTGGCTTGTTTAAGAAGCCGAAAAACCATGCTGACACTAAATGGATAAACGCGAAGCTGCTTCAGCTTAGTGAGGAGACTTATGGAGCACACCTATGGAACAAGCAAACTTACTTTGCCTATCAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

318

Amino Acids

36.3

Weight (kDa)

9.07

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 132 - 214 2.2e-17 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 252 - 311 5.3e-09 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38152
fragaria_vesca FvH4_6g11720
malus_domestica MD04G1149800.v1.1 MD12G1164200.v1.1
prunus_persica Prupe.6G274700_v2.0.a1
pyrus_communis pycom04g13530
rosa_chinensis RchiOBHm_Chr3g0463491
rosa_laevigata RLG00000019555 RLG00000024783
rosa_multiflora Rmu_sc0000923.1_g000020
rosa_roxburghii Rroxscaffold_6G00416520
rosa_rugosa Rorug03G0065100
rosa_samantha Rh3AG122300 Rh3BG127000 Rh3CG128900 Rh3DG127800
rosa_wichuraiana Rw0G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 719
AccII CGCG 2 cut(s) 399, 875
AciI CCGC 3 cut(s) 22, 399, 650
AcsI RAATTY 3 cut(s) 299, 374, 722
AcuI CTGAAG 1 cut(s) 869
AcyI GRCGYC 1 cut(s) 422
AfaI GTAC 2 cut(s) 575, 643
AfeI AGCGCT 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 385, 583
AgsI TTSAA 4 cut(s) 139, 299, 406, 445
AjnI CCWGG 5 cut(s) 33, 464, 499, 576, 760
AluBI AGCT 5 cut(s) 149, 458, 518, 880, 889
AluI AGCT 5 cut(s) 149, 458, 518, 880, 889
Alw21I GWGCWC 2 cut(s) 637, 913
Alw26I GTCTC 4 cut(s) 314, 489, 535, 893
Alw44I GTGCAC 1 cut(s) 633
AlwNI CAGNNNCTG 2 cut(s) 163, 526
Aor51HI AGCGCT 1 cut(s) 219
AoxI GGCC 1 cut(s) 462
ApaLI GTGCAC 1 cut(s) 633
ApeKI GCWGC 4 cut(s) 163, 220, 274, 880
ApoI RAATTY 3 cut(s) 299, 374, 722
AspLEI GCGC 3 cut(s) 220, 274, 720
AspS9I GGNCC 2 cut(s) 666, 739
AsuHPI GGTGA 1 cut(s) 515
AvaII GGWCC 2 cut(s) 666, 739
BaeGI GKGCMC 1 cut(s) 637
BbsI GAAGAC 1 cut(s) 488
Bbv12I GWGCWC 2 cut(s) 637, 913
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 4 cut(s) 150, 207, 261, 867
BccI CCATC 2 cut(s) 92, 379
BcgI CGANNNNNNTGC 2 cut(s) 709, 743
BciT130I CCWGG 5 cut(s) 35, 466, 501, 578, 762
BcoDI GTCTC 4 cut(s) 314, 489, 535, 893
BfaI CTAG 3 cut(s) 545, 816, 952
BfmI CTRYAG 2 cut(s) 164, 275
BfoI RGCGCY 2 cut(s) 221, 275
BisI GCNGC 5 cut(s) 22, 164, 221, 275, 881
BlsI GCNGC 5 cut(s) 23, 165, 222, 276, 882
Bme1390I CCNGG 5 cut(s) 35, 466, 501, 578, 762
Bme18I GGWCC 2 cut(s) 666, 739
BmgT120I GGNCC 2 cut(s) 666, 739
BmrFI CCNGG 5 cut(s) 35, 466, 501, 578, 762
BmsI GCATC 1 cut(s) 637
BpiI GAAGAC 1 cut(s) 488
Bpu10I CCTNAGC 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 702
BsaHI GRCGYC 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 1 cut(s) 499
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 583
Bse1I ACTGG 3 cut(s) 187, 668, 803
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 5 cut(s) 35, 466, 501, 578, 762
BseDI CCNNGG 1 cut(s) 499
BseGI GGATG 2 cut(s) 652, 706
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 2 cut(s) 385, 583
BseMII CTCAG 6 cut(s) 141, 170, 338, 450, 465, 533
BseNI ACTGG 3 cut(s) 187, 668, 803
BseRI GAGGAG 1 cut(s) 911
BseSI GKGCMC 1 cut(s) 637
BseXI GCAGC 4 cut(s) 150, 207, 261, 867
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 399, 875
BshFI GGCC 1 cut(s) 464
BsiHKAI GWGCWC 2 cut(s) 637, 913
BslI CCNNNNNNNGG 2 cut(s) 385, 583
BsmAI GTCTC 4 cut(s) 314, 489, 535, 893
BsnI GGCC 1 cut(s) 464
Bso31I GGTCTC 1 cut(s) 489
Bsp1286I GDGCHC 2 cut(s) 637, 913
Bsp143I GATC 2 cut(s) 75, 394
BspACI CCGC 3 cut(s) 22, 399, 650
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 6 cut(s) 140, 169, 337, 451, 466, 532
BspFNI CGCG 2 cut(s) 399, 875
BspHI TCATGA 1 cut(s) 333
BspMAI CTGCAG 2 cut(s) 168, 279
BspTNI GGTCTC 1 cut(s) 489
BsrI ACTGG 3 cut(s) 187, 668, 803
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 2 cut(s) 75, 394
BssNI GRCGYC 1 cut(s) 422
Bst2UI CCWGG 5 cut(s) 35, 466, 501, 578, 762
Bst4CI ACNGT 2 cut(s) 61, 778
Bst6I CTCTTC 1 cut(s) 717
BstACI GRCGYC 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 8 cut(s) 29, 127, 156, 324, 459, 474, 519, 890
BstF5I GGATG 2 cut(s) 652, 706
BstFNI CGCG 2 cut(s) 399, 875
BstH2I RGCGCY 2 cut(s) 221, 275
BstHHI GCGC 3 cut(s) 220, 274, 720
BstKTI GATC 2 cut(s) 78, 397
BstMAI GTCTC 4 cut(s) 314, 489, 535, 893
BstMBI GATC 2 cut(s) 75, 394
BstMWI GCNNNNNNNGC 4 cut(s) 30, 229, 470, 886
BstNI CCWGG 5 cut(s) 35, 466, 501, 578, 762
BstSCI CCNGG 5 cut(s) 33, 464, 499, 576, 760
BstSFI CTRYAG 2 cut(s) 164, 275
BstSLI GKGCMC 1 cut(s) 637
BstUI CGCG 2 cut(s) 399, 875
BstV1I GCAGC 4 cut(s) 150, 207, 261, 867
BstV2I GAAGAC 1 cut(s) 488
BstXI CCANNNNNNTGG 1 cut(s) 810
BsuRI GGCC 1 cut(s) 464
BtsCI GGATG 2 cut(s) 652, 706
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 4 cut(s) 57, 637, 690, 810
Cac8I GCNNGC 1 cut(s) 351
CaiI CAGNNNCTG 2 cut(s) 163, 526
CciI TCATGA 1 cut(s) 333
CfoI GCGC 3 cut(s) 220, 274, 720
Cfr13I GGNCC 2 cut(s) 666, 739
CseI GACGC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 574, 642
CviAII CATG 5 cut(s) 284, 334, 339, 413, 851
CviQI GTAC 2 cut(s) 574, 642
DdeI CTNAG 8 cut(s) 29, 127, 156, 324, 459, 474, 519, 890
DpnI GATC 2 cut(s) 77, 396
DpnII GATC 2 cut(s) 75, 394
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
Eco147I AGGCCT 1 cut(s) 464
Eco31I GGTCTC 1 cut(s) 489
Eco32I GATATC 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 666, 739
Eco47III AGCGCT 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 374
EcoRII CCWGG 5 cut(s) 33, 464, 499, 576, 760
EcoRV GATATC 1 cut(s) 345
FaeI CATG 5 cut(s) 287, 337, 342, 416, 854
FatI CATG 5 cut(s) 283, 333, 338, 412, 850
FauI CCCGC 1 cut(s) 392
Fnu4HI GCNGC 5 cut(s) 22, 164, 221, 275, 881
FokI GGATG 2 cut(s) 659, 693
Fsp4HI GCNGC 5 cut(s) 22, 164, 221, 275, 881
FspBI CTAG 3 cut(s) 545, 816, 952
FspI TGCGCA 1 cut(s) 719
GlaI GCGC 3 cut(s) 219, 273, 719
GluI GCNGC 5 cut(s) 22, 164, 221, 275, 881
HaeII RGCGCY 2 cut(s) 221, 275
HaeIII GGCC 1 cut(s) 464
HgaI GACGC 1 cut(s) 430
HhaI GCGC 3 cut(s) 220, 274, 720
Hin1I GRCGYC 1 cut(s) 422
Hin1II CATG 5 cut(s) 287, 337, 342, 416, 854
Hin6I GCGC 3 cut(s) 218, 272, 718
HinP1I GCGC 3 cut(s) 218, 272, 718
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 6 cut(s) 12, 116, 169, 322, 508, 703
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 3 cut(s) 262, 574, 635
Hpy188I TCNGA 2 cut(s) 202, 522
Hpy188III TCNNGA 4 cut(s) 296, 334, 445, 598
Hpy8I GTNNAC 3 cut(s) 262, 574, 635
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 2 cut(s) 61, 778
HpyCH4V TGCA 8 cut(s) 57, 95, 166, 232, 277, 635, 683, 751
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 229, 470, 886
HpyF3I CTNAG 8 cut(s) 29, 127, 156, 324, 459, 474, 519, 890
Hsp92I GRCGYC 1 cut(s) 422
Hsp92II CATG 5 cut(s) 287, 337, 342, 416, 854
HspAI GCGC 3 cut(s) 218, 272, 718
Kzo9I GATC 2 cut(s) 75, 394
LmnI GCTCC 1 cut(s) 908
Lsp1109I GCAGC 4 cut(s) 150, 207, 261, 867
LweI GCATC 1 cut(s) 637
MaeI CTAG 3 cut(s) 545, 816, 952
MaeIII GTNAC 2 cut(s) 418, 620
MalI GATC 2 cut(s) 77, 396
MboI GATC 2 cut(s) 75, 394
MboII GAAGA 5 cut(s) 43, 73, 322, 493, 704
MhlI GDGCHC 2 cut(s) 637, 913
MluCI AATT 5 cut(s) 299, 374, 532, 722, 819
MlyI GAGTC 2 cut(s) 163, 316
MnlI CCTC 7 cut(s) 348, 454, 560, 720, 724, 795, 889
MseI TTAA 2 cut(s) 255, 834
MslI CAYNNNNRTG 3 cut(s) 315, 596, 916
MspA1I CMGCKG 1 cut(s) 458
MspR9I CCNGG 5 cut(s) 35, 466, 501, 578, 762
MvaI CCWGG 5 cut(s) 35, 466, 501, 578, 762
MvnI CGCG 2 cut(s) 399, 875
MwoI GCNNNNNNNGC 4 cut(s) 30, 229, 470, 886
NdeII GATC 2 cut(s) 75, 394
NlaIII CATG 5 cut(s) 287, 337, 342, 416, 854
NmuCI GTSAC 1 cut(s) 418
NsbI TGCGCA 1 cut(s) 719
PagI TCATGA 1 cut(s) 333
PceI AGGCCT 1 cut(s) 464
PfeI GAWTC 4 cut(s) 12, 116, 508, 703
PkrI GCNGC 5 cut(s) 23, 165, 222, 276, 882
PleI GAGTC 2 cut(s) 163, 316
PpsI GAGTC 2 cut(s) 163, 316
Psp6I CCWGG 5 cut(s) 33, 464, 499, 576, 760
PspGI CCWGG 5 cut(s) 33, 464, 499, 576, 760
PspPI GGNCC 2 cut(s) 666, 739
PstI CTGCAG 2 cut(s) 168, 279
PstNI CAGNNNCTG 2 cut(s) 163, 526
PvuII CAGCTG 1 cut(s) 458
RsaI GTAC 2 cut(s) 575, 643
RsaNI GTAC 2 cut(s) 574, 642
RseI CAYNNNNRTG 3 cut(s) 315, 596, 916
SaqAI TTAA 2 cut(s) 255, 834
SatI GCNGC 5 cut(s) 22, 164, 221, 275, 881
Sau3AI GATC 2 cut(s) 75, 394
Sau96I GGNCC 2 cut(s) 666, 739
SchI GAGTC 2 cut(s) 163, 316
ScrFI CCNGG 5 cut(s) 35, 466, 501, 578, 762
SduI GDGCHC 2 cut(s) 637, 913
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 2 cut(s) 164, 275
SinI GGWCC 2 cut(s) 666, 739
SmiMI CAYNNNNRTG 3 cut(s) 315, 596, 916
Sse9I AATT 5 cut(s) 299, 374, 532, 722, 819
SseBI AGGCCT 1 cut(s) 464
SsiI CCGC 3 cut(s) 22, 399, 650
SspMI CTAG 3 cut(s) 545, 816, 952
StuI AGGCCT 1 cut(s) 464
StyD4I CCNGG 5 cut(s) 33, 464, 499, 576, 760
TaaI ACNGT 2 cut(s) 61, 778
TaqI TCGA 5 cut(s) 15, 180, 383, 656, 729
TasI AATT 5 cut(s) 299, 374, 532, 722, 819
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 4 cut(s) 12, 116, 508, 703
Tru1I TTAA 2 cut(s) 255, 834
Tru9I TTAA 2 cut(s) 255, 834
TscAI CASTG 4 cut(s) 64, 637, 690, 810
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 4 cut(s) 163, 220, 274, 880
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 9 cut(s) 73, 181, 272, 322, 401, 494, 580, 715, 716
TspGWI ACGGA 2 cut(s) 599, 659
TspRI CASTG 4 cut(s) 64, 637, 690, 810
VneI GTGCAC 1 cut(s) 633
VpaK11BI GGWCC 2 cut(s) 666, 739
XapI RAATTY 3 cut(s) 299, 374, 722
XspI CTAG 3 cut(s) 545, 816, 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.