MD12G1164200.v1.1

Lactosylceramide 4-alpha-galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013878
Physical Location & Seq
Reverse (-)
24554171 .. 24555430
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1164200.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGACTCGAATCGACAGCTGCCTACTTAGCCTCGCCAACTTTCACGTCTTCTGCACTCTTTCAATTGTTGCAATCATCTTCATCGTCAATGCCGATAAAATCATCTATAGCAACTCGGAACGGTCATTGGCGTCGGACAATGAAGCGATTTCTCCGTTGCTGCAGAATTATGATCATAATTCTGCAGGCAGACTGATCAAGTCTGATGTAAGTCGCTTAGTCCTGCATTCCATGCAGGACGAGACCATTGAAGTTGGTAGAGAAAACACAAGGCCTCTTGTTCCTCCTTTCAATGTCACAGAAGAAGAGAGAATTGCTTGGTTCCGAAGCAAGCTGCCGGAATTTCACATTCTCAAGTCAGACAAGTCTGCAAAGCAATTCCATGGCCGTGTTCTGGAATTCTTTAACAAGGAGTGTGAGGGTCAGTTCTTTATGACATGGATTTCGCCGGCAAGGTCTTTTGGGAGCAGAGAGTTCTTGACTATGGAAAGTCTCTTCAAAGCCCACCCACGCGGATGTTTGATGATTATCTCGAGAAGTATGGACTCCAAGCACGGCTACAGAATTCTGAAACCGCTGCAGGATAGTGGGTTCAAAGTTCATGCTGTGACACCAGACTTGTCATTTCTGTTCAACAACACACCGGCAAAAACCTGGTTTGCTGGGATGAAGAGAGGCAACAGAGACCCTGGTGAGATTCCATTAGCCCAGAACCTGTCGAATTTGATTAGACTTGCAGTTCTATACAAGTATGGAGGAGTTTATTTAGACACAGACTTCATCGTCTTGAAATCATTCTCAGGACTGAGAAACTCGATTGGTGCACAAAGTGCGGATGTGGTTTCGAAGAACTGGACCAGATTAAATAATGCAGTTCTGATCTTTGATGCGAATCATCCCCTTCTGCACAAGTTCATGAAGGAATTTGCCCTGACTTTTGATGGGAATAAATGGGGGCATAATGGTCCCTACCTGGTCTCCAGAGTGGTCCTGAGGGTGCAAAACCGACCCGGTTATAACTTCACGATTCTACCCCCTCTCGCCTTTTACCCTGTCGACTGGAATAGGATCGGGGGGCTGTTTAAAAAGCCGGGAAGCCCGGCTCATTCACGATGGATAAAAGCAAAGCTGCTTCAGCTTAGCGGGGAGACTTATGGAGTACACCTATGGAACAAGCAAAGCAGCAGAGAAGTAGTCCAAGAAGGAAGCATTATGCAGAGATTAATCTCCGATCACTGCATCATTTGCAACGACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

420

Amino Acids

47.68

Weight (kDa)

9.39

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 157 - 277 6.5e-22 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 295 - 417 8.1e-28 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38152
fragaria_vesca FvH4_6g11720
malus_domestica MD04G1149800.v1.1 MD12G1164200.v1.1
prunus_persica Prupe.6G274700_v2.0.a1
pyrus_communis pycom04g13530
rosa_chinensis RchiOBHm_Chr3g0463491
rosa_laevigata RLG00000019555 RLG00000024783
rosa_multiflora Rmu_sc0000923.1_g000020
rosa_roxburghii Rroxscaffold_6G00416520
rosa_rugosa Rorug03G0065100
rosa_samantha Rh3AG122300 Rh3BG127000 Rh3CG128900 Rh3DG127800
rosa_wichuraiana Rw0G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
AasI GACNNNNNNGTC 1 cut(s) 782
AccI GTMKAC 1 cut(s) 1056
AccII CGCG 1 cut(s) 513
AciI CCGC 4 cut(s) 513, 575, 833, 1143
AclWI GGATC 1 cut(s) 1076
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 5 cut(s) 341, 398, 564, 721, 923
AcuI CTGAAG 1 cut(s) 1118
AcyI GRCGYC 1 cut(s) 131
AdeI CACNNNGTG 1 cut(s) 830
AfaI GTAC 1 cut(s) 1161
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 7 cut(s) 63, 251, 292, 499, 595, 634, 790
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 3 cut(s) 653, 688, 972
AloI GAACNNNNNNTCC 2 cut(s) 575, 607
AluBI AGCT 4 cut(s) 18, 334, 1129, 1138
AluI AGCT 4 cut(s) 18, 334, 1129, 1138
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 5 cut(s) 236, 497, 678, 982, 1142
Alw44I GTGCAC 1 cut(s) 822
AlwI GGATC 1 cut(s) 1076
AlwNI CAGNNNCTG 1 cut(s) 715
Ama87I CYCGRG 1 cut(s) 532
AoxI GGCC 2 cut(s) 272, 385
ApaLI GTGCAC 1 cut(s) 822
ApeKI GCWGC 6 cut(s) 18, 160, 334, 577, 1129, 1182
ApoI RAATTY 5 cut(s) 341, 398, 564, 721, 923
ArsI GACNNNNNNTTYG 2 cut(s) 427, 459
AseI ATTAAT 1 cut(s) 1223
Asp700I GAANNNNTTC 1 cut(s) 794
AspS9I GGNCC 3 cut(s) 855, 965, 988
AsuC2I CCSGG 3 cut(s) 1011, 1092, 1100
AsuHPI GGTGA 1 cut(s) 704
AsuII TTCGAA 1 cut(s) 845
AvaI CYCGRG 1 cut(s) 532
AvaII GGWCC 3 cut(s) 855, 965, 988
AxyI CCTNAGG 1 cut(s) 992
BaeGI GKGCMC 1 cut(s) 826
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 826
BbvI GCAGC 6 cut(s) 5, 147, 321, 564, 1116, 1194
BccI CCATC 2 cut(s) 935, 1107
BceAI ACGGC 2 cut(s) 372, 571
BciT130I CCWGG 3 cut(s) 655, 690, 974
BclI TGATCA 2 cut(s) 172, 195
BcnI CCSGG 3 cut(s) 1011, 1092, 1100
BcoDI GTCTC 5 cut(s) 236, 497, 678, 982, 1142
BfmI CTRYAG 5 cut(s) 106, 161, 183, 559, 578
BisI GCNGC 6 cut(s) 19, 161, 335, 578, 1130, 1183
BlpI GCTNAGC 1 cut(s) 1139
BlsI GCNGC 6 cut(s) 20, 162, 336, 579, 1131, 1184
Bme1390I CCNGG 6 cut(s) 655, 690, 974, 1011, 1092, 1100
Bme18I GGWCC 3 cut(s) 855, 965, 988
BmeT110I CYCGRG 1 cut(s) 532
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 855, 965, 988
BmiI GGNNCC 2 cut(s) 323, 967
BmrFI CCNGG 6 cut(s) 655, 690, 974, 1011, 1092, 1100
BmsI GCATC 2 cut(s) 877, 1248
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 964
Bpu1102I GCTNAGC 1 cut(s) 1139
Bpu14I TTCGAA 1 cut(s) 845
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 3 cut(s) 1011, 1092, 1100
BsaBI GATNNNNATC 2 cut(s) 527, 891
BsaHI GRCGYC 1 cut(s) 131
BsaI GGTCTC 3 cut(s) 236, 678, 982
BsaJI CCNNGG 2 cut(s) 382, 688
BsaXI ACNNNNNCTCC 2 cut(s) 962, 992
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse118I RCCGGY 2 cut(s) 448, 643
Bse1I ACTGG 2 cut(s) 857, 1064
Bse21I CCTNAGG 1 cut(s) 992
Bse8I GATNNNNATC 2 cut(s) 527, 891
BseBI CCWGG 3 cut(s) 655, 690, 974
BseDI CCNNGG 2 cut(s) 382, 688
BseGI GGATG 4 cut(s) 521, 672, 841, 895
BseJI GATNNNNATC 2 cut(s) 527, 891
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMII CTCAG 3 cut(s) 797, 813, 983
BseNI ACTGG 2 cut(s) 857, 1064
BseRI GAGGAG 1 cut(s) 771
BseSI GKGCMC 1 cut(s) 826
BseXI GCAGC 6 cut(s) 5, 147, 321, 564, 1116, 1194
BseYI CCCAGC 1 cut(s) 662
BsgI GTGCAG 2 cut(s) 37, 890
Bsh1236I CGCG 1 cut(s) 513
BshFI GGCC 2 cut(s) 274, 387
BsiHKAI GWGCWC 1 cut(s) 826
BsiHKCI CYCGRG 1 cut(s) 532
BsiSI CCGG 6 cut(s) 338, 449, 644, 1011, 1091, 1100
BslFI GGGAC 1 cut(s) 951
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 5 cut(s) 236, 497, 678, 982, 1142
BsmFI GGGAC 1 cut(s) 951
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 2 cut(s) 274, 387
Bso31I GGTCTC 3 cut(s) 236, 678, 982
BsoBI CYCGRG 1 cut(s) 532
Bsp119I TTCGAA 1 cut(s) 845
Bsp1286I GDGCHC 1 cut(s) 826
Bsp143I GATC 5 cut(s) 172, 195, 879, 1068, 1231
Bsp1720I GCTNAGC 1 cut(s) 1139
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 4 cut(s) 513, 575, 833, 1143
BspANI GGCC 2 cut(s) 274, 387
BspCNI CTCAG 3 cut(s) 798, 812, 984
BspFNI CGCG 1 cut(s) 513
BspHI TCATGA 1 cut(s) 915
BspLI GGNNCC 2 cut(s) 323, 967
BspMAI CTGCAG 3 cut(s) 165, 187, 582
BspPI GGATC 1 cut(s) 1076
BspT104I TTCGAA 1 cut(s) 845
BspTNI GGTCTC 3 cut(s) 236, 678, 982
BsrFI RCCGGY 2 cut(s) 448, 643
BsrI ACTGG 2 cut(s) 857, 1064
BssAI RCCGGY 2 cut(s) 448, 643
BssECI CCNNGG 2 cut(s) 382, 688
BssMI GATC 5 cut(s) 172, 195, 879, 1068, 1231
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 382
Bst2UI CCWGG 3 cut(s) 655, 690, 974
Bst4CI ACNGT 1 cut(s) 123
Bst6I CTCTTC 3 cut(s) 300, 500, 665
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 3 cut(s) 232, 830, 1245
BstBI TTCGAA 1 cut(s) 845
BstC8I GCNNGC 3 cut(s) 187, 332, 450
BstDEI CTNAG 6 cut(s) 26, 217, 799, 806, 992, 1139
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 4 cut(s) 521, 672, 841, 895
BstFNI CGCG 1 cut(s) 513
BstKTI GATC 5 cut(s) 175, 198, 882, 1071, 1234
BstMAI GTCTC 5 cut(s) 236, 497, 678, 982, 1142
BstMBI GATC 5 cut(s) 172, 195, 879, 1068, 1231
BstMWI GCNNNNNNNGC 5 cut(s) 27, 232, 830, 1135, 1245
BstNI CCWGG 3 cut(s) 655, 690, 974
BstSCI CCNGG 6 cut(s) 653, 688, 972, 1009, 1090, 1098
BstSFI CTRYAG 5 cut(s) 106, 161, 183, 559, 578
BstSLI GKGCMC 1 cut(s) 826
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 6 cut(s) 5, 147, 321, 564, 1116, 1194
BstV2I GAAGAC 1 cut(s) 40
Bsu36I CCTNAGG 1 cut(s) 992
BsuRI GGCC 2 cut(s) 274, 387
BtgI CCRYGG 1 cut(s) 382
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 4 cut(s) 521, 672, 841, 895
BtsI GCAGTG 1 cut(s) 1234
BtsIMutI CAGTG 1 cut(s) 1234
Cac8I GCNNGC 3 cut(s) 187, 332, 450
CaiI CAGNNNCTG 1 cut(s) 715
CciI TCATGA 1 cut(s) 915
Cfr10I RCCGGY 2 cut(s) 448, 643
Cfr13I GGNCC 3 cut(s) 855, 965, 988
CseI GACGC 1 cut(s) 120
CsiI ACCWGGT 2 cut(s) 653, 972
Csp6I GTAC 1 cut(s) 1160
CviAII CATG 5 cut(s) 232, 383, 438, 602, 916
CviQI GTAC 1 cut(s) 1160
DdeI CTNAG 6 cut(s) 26, 217, 799, 806, 992, 1139
DpnI GATC 5 cut(s) 174, 197, 881, 1070, 1233
DpnII GATC 5 cut(s) 172, 195, 879, 1068, 1231
DraI TTTAAA 1 cut(s) 1084
DraIII CACNNNGTG 1 cut(s) 830
DrdI GACNNNNNNGTC 1 cut(s) 782
DseDI GACNNNNNNGTC 1 cut(s) 782
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 3 cut(s) 300, 500, 665
EarI CTCTTC 3 cut(s) 300, 500, 665
Eco130I CCWWGG 1 cut(s) 382
Eco147I AGGCCT 1 cut(s) 274
Eco31I GGTCTC 3 cut(s) 236, 678, 982
Eco47I GGWCC 3 cut(s) 855, 965, 988
Eco57I CTGAAG 1 cut(s) 1118
Eco81I CCTNAGG 1 cut(s) 992
Eco88I CYCGRG 1 cut(s) 532
EcoRI GAATTC 2 cut(s) 398, 564
EcoRII CCWGG 3 cut(s) 653, 688, 972
EcoT14I CCWWGG 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 5 cut(s) 235, 386, 441, 605, 919
FaqI GGGAC 1 cut(s) 951
FatI CATG 5 cut(s) 231, 382, 437, 601, 915
FauI CCCGC 1 cut(s) 1136
FbaI TGATCA 2 cut(s) 172, 195
FblI GTMKAC 1 cut(s) 1056
Fnu4HI GCNGC 6 cut(s) 19, 161, 335, 578, 1130, 1183
FokI GGATG 4 cut(s) 528, 679, 848, 882
Fsp4HI GCNGC 6 cut(s) 19, 161, 335, 578, 1130, 1183
GluI GCNGC 6 cut(s) 19, 161, 335, 578, 1130, 1183
GsaI CCCAGC 1 cut(s) 666
GsuI CTGGAG 1 cut(s) 964
HaeIII GGCC 2 cut(s) 274, 387
HapII CCGG 6 cut(s) 338, 449, 644, 1011, 1091, 1100
HgaI GACGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 5 cut(s) 235, 386, 441, 605, 919
HincII GTYRAC 1 cut(s) 1057
HindII GTYRAC 1 cut(s) 1057
HinfI GANTC 6 cut(s) 4, 9, 545, 697, 892, 1027
HpaII CCGG 6 cut(s) 338, 449, 644, 1011, 1091, 1100
HphI GGTGA 1 cut(s) 704
Hpy166II GTNNAC 3 cut(s) 824, 1057, 1162
Hpy188I TCNGA 8 cut(s) 118, 136, 205, 326, 361, 570, 879, 1231
Hpy8I GTNNAC 3 cut(s) 824, 1057, 1162
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 3 cut(s) 911, 913, 1196
HpyCH4III ACNGT 1 cut(s) 123
HpyCH4IV ACGT 1 cut(s) 45
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 232, 830, 1135, 1245
HpyF3I CTNAG 6 cut(s) 26, 217, 799, 806, 992, 1139
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 5 cut(s) 235, 386, 441, 605, 919
KroI GCCGGC 1 cut(s) 448
KroNI GCCGGC 1 cut(s) 450
Ksp22I TGATCA 2 cut(s) 172, 195
Kzo9I GATC 5 cut(s) 172, 195, 879, 1068, 1231
LmnI GCTCC 1 cut(s) 465
Lsp1109I GCAGC 6 cut(s) 5, 147, 321, 564, 1116, 1194
LweI GCATC 2 cut(s) 877, 1248
MabI ACCWGGT 2 cut(s) 653, 972
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 2 cut(s) 295, 607
MalI GATC 5 cut(s) 174, 197, 881, 1070, 1233
MboI GATC 5 cut(s) 172, 195, 879, 1068, 1231
MboII GAAGA 7 cut(s) 40, 70, 314, 317, 487, 682, 859
MfeI CAATTG 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 826
MlyI GAGTC 1 cut(s) 539
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 8 cut(s) 41, 285, 294, 412, 668, 749, 987, 1047
MroNI GCCGGC 1 cut(s) 448
MroXI GAANNNNTTC 1 cut(s) 794
MseI TTAA 4 cut(s) 405, 863, 1083, 1223
MslI CAYNNNNRTG 2 cut(s) 387, 514
MspA1I CMGCKG 2 cut(s) 18, 577
MspI CCGG 6 cut(s) 338, 449, 644, 1011, 1091, 1100
MspR9I CCNGG 6 cut(s) 655, 690, 974, 1011, 1092, 1100
MunI CAATTG 1 cut(s) 63
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 3 cut(s) 655, 690, 974
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 5 cut(s) 27, 232, 830, 1135, 1245
NaeI GCCGGC 1 cut(s) 450
NciI CCSGG 3 cut(s) 1011, 1092, 1100
NcoI CCATGG 1 cut(s) 382
NdeII GATC 5 cut(s) 172, 195, 879, 1068, 1231
NgoMIV GCCGGC 1 cut(s) 448
NlaIII CATG 5 cut(s) 235, 386, 441, 605, 919
NlaIV GGNNCC 2 cut(s) 323, 967
NmuCI GTSAC 2 cut(s) 295, 607
NspV TTCGAA 1 cut(s) 845
PaeR7I CTCGAG 1 cut(s) 532
PagI TCATGA 1 cut(s) 915
PceI AGGCCT 1 cut(s) 274
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 794
PfeI GAWTC 4 cut(s) 9, 697, 892, 1027
PkrI GCNGC 6 cut(s) 20, 162, 336, 579, 1131, 1184
PleI GAGTC 1 cut(s) 539
PpsI GAGTC 1 cut(s) 539
PshBI ATTAAT 1 cut(s) 1223
PsiI TTATAA 1 cut(s) 1017
Psp6I CCWGG 3 cut(s) 653, 688, 972
PspFI CCCAGC 1 cut(s) 662
PspGI CCWGG 3 cut(s) 653, 688, 972
PspN4I GGNNCC 2 cut(s) 323, 967
PspPI GGNCC 3 cut(s) 855, 965, 988
PstI CTGCAG 3 cut(s) 165, 187, 582
PstNI CAGNNNCTG 1 cut(s) 715
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 1 cut(s) 1161
RsaNI GTAC 1 cut(s) 1160
RseI CAYNNNNRTG 2 cut(s) 387, 514
SalI GTCGAC 1 cut(s) 1055
SaqAI TTAA 4 cut(s) 405, 863, 1083, 1223
SatI GCNGC 6 cut(s) 19, 161, 335, 578, 1130, 1183
Sau3AI GATC 5 cut(s) 172, 195, 879, 1068, 1231
Sau96I GGNCC 3 cut(s) 855, 965, 988
SchI GAGTC 1 cut(s) 539
ScrFI CCNGG 6 cut(s) 655, 690, 974, 1011, 1092, 1100
SduI GDGCHC 1 cut(s) 826
SexAI ACCWGGT 2 cut(s) 653, 972
SfaNI GCATC 2 cut(s) 877, 1248
SfcI CTRYAG 5 cut(s) 106, 161, 183, 559, 578
Sfr274I CTCGAG 1 cut(s) 532
SfuI TTCGAA 1 cut(s) 845
SinI GGWCC 3 cut(s) 855, 965, 988
SlaI CTCGAG 1 cut(s) 532
SmiMI CAYNNNNRTG 2 cut(s) 387, 514
SmlI CTYRAG 2 cut(s) 353, 532
SmoI CTYRAG 2 cut(s) 353, 532
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 4 cut(s) 513, 575, 833, 1143
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 6 cut(s) 653, 688, 972, 1009, 1090, 1098
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 1 cut(s) 123
TaiI ACGT 1 cut(s) 48
TaqI TCGA 7 cut(s) 7, 12, 533, 719, 815, 845, 1056
TatI WGTACW 1 cut(s) 1159
TfiI GAWTC 4 cut(s) 9, 697, 892, 1027
Tru1I TTAA 4 cut(s) 405, 863, 1083, 1223
Tru9I TTAA 4 cut(s) 405, 863, 1083, 1223
TscAI CASTG 1 cut(s) 1241
TseFI GTSAC 2 cut(s) 295, 607
TseI GCWGC 6 cut(s) 18, 160, 334, 577, 1129, 1182
Tsp45I GTSAC 2 cut(s) 295, 607
TspDTI ATGAA 7 cut(s) 70, 156, 590, 683, 769, 904, 932
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 1 cut(s) 1241
VneI GTGCAC 1 cut(s) 822
VpaK11BI GGWCC 3 cut(s) 855, 965, 988
VspI ATTAAT 1 cut(s) 1223
XapI RAATTY 5 cut(s) 341, 398, 564, 721, 923
XhoI CTCGAG 1 cut(s) 532
XmiI GTMKAC 1 cut(s) 1056
XmnI GAANNNNTTC 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.