RLG00000024783

Lactosylceramide 4-alpha-galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013878
Physical Location & Seq
Reverse (-)
39107028 .. 39108302
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024783

Sequence Viewer

Length: 1179 bp
ATGAGTAGCCGAATCGACAGCCGCCTACTTAGCCTGGCGAAGTTCCACATCTTCTGCACTGTATCACTTGTTGCAATCATCTTCATCATCTATGCAGATGGTATCATATATACTGATTCCCAAAACTCGGTTGAGTTGAAAGATAAAGCTGTTTCTCAGGTGATGCAGACTCATAGTATCGAACGCCAGTTCATCAAGTCTGATATAACTCGTAAAGCGCTGCGTTCTATGCAAGAAAATGTCCTTGATGTCGTTAAAGTTGATACAGAAGATACAAGTCCTGTTGTTGCTCCTTTCAATGTCACTGAAGAAGAAAGAATCAGCTGGTTTCGGGGAAAGCTGCCCGAATTTGTGATTTTTGATTCGGACGAGAAGGCGCTGCAGTTTCATGGTAGAGTTCTTGAATTCTTTGACCATAAGTGTGAGACTCAGTTCTTCATGACATGGATATCGCCTGCGAGGCGAGGTTACAGGCTTCTAAAACCGCTGCAGGATCGCGGGTTCAAAGTTCATGCTGTGACGCCAGACTTGTCATTTCTGTTCAAGAACACCCCAGCTGAGGCCTGGTTTGCTGAGATGAAGACAGGCAACAGAGACCCTGGTGAGATTCCATTAGCTCAGAACCTGTCTAATTTATTGAGACTAGCAGTTTTATACAAGTATGGAGGTGTGTACCTGGACACGGATTTCATTGTCGTGAAATCATTTTCGGGGTTGAGGAACTCGATCGGTGCACAAAGTATGGATGCGGTGTCGAAAAACTGGACCAGACTGAACAATGCAGTGCTGGTGTTTGATATGAATCATCCTCTTCTGCGCAAATTCATCGAGGAGTTTGCCTTGACTTTTGATGGGAATAAATGGGGTCACAATGGACCCTATCTTGTTTCCAGAGTGGTCCAAAGAGTGCAAAACAGACCTGGATATAACTTTACGGTCCTGCCTCCAATGGCTTTCTATCCAGTGGATTGGACTAGAATTGGGGGCTTGTTTAAGAAGCCGAAAAACCATGCTGACACTAAATGGATAAACGCGAAGCTGCTTCAGCTTAGTGAGGAGACTTATGGAGTACACCTATGGAACAAGCAAAGTAGCAGAGTACTAATCGAAGAAGGAAGCATTATGCAGAGATTAATCTCCGATCACTGCGTCATCTGTAAAGACATATATAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

393

Amino Acids

45.06

Weight (kDa)

8.85

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 168 - 247 5.2e-18 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 265 - 387 6.4e-30 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38152
fragaria_vesca FvH4_6g11720
malus_domestica MD04G1149800.v1.1 MD12G1164200.v1.1
prunus_persica Prupe.6G274700_v2.0.a1
pyrus_communis pycom04g13530
rosa_chinensis RchiOBHm_Chr3g0463491
rosa_laevigata RLG00000019555 RLG00000024783
rosa_multiflora Rmu_sc0000923.1_g000020
rosa_roxburghii Rroxscaffold_6G00416520
rosa_rugosa Rorug03G0065100
rosa_samantha Rh3AG122300 Rh3BG127000 Rh3CG128900 Rh3DG127800
rosa_wichuraiana Rw0G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 818
AccII CGCG 2 cut(s) 498, 1034
AciI CCGC 4 cut(s) 22, 485, 498, 749
AclWI GGATC 1 cut(s) 501
AcsI RAATTY 3 cut(s) 347, 404, 821
AcuI CTGAAG 2 cut(s) 327, 1028
AcyI GRCGYC 1 cut(s) 521
AfaI GTAC 3 cut(s) 674, 1071, 1101
AfeI AGCGCT 1 cut(s) 219
AfiI CCNNNNNNNGG 3 cut(s) 127, 559, 682
AgsI TTSAA 5 cut(s) 139, 298, 404, 505, 544
AjnI CCWGG 5 cut(s) 33, 563, 598, 675, 919
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
AluBI AGCT 7 cut(s) 149, 324, 340, 557, 617, 1039, 1048
AluI AGCT 7 cut(s) 149, 324, 340, 557, 617, 1039, 1048
Alw21I GWGCWC 1 cut(s) 736
Alw26I GTCTC 4 cut(s) 419, 588, 634, 1052
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 1 cut(s) 501
AlwNI CAGNNNCTG 1 cut(s) 625
Aor51HI AGCGCT 1 cut(s) 219
AoxI GGCC 1 cut(s) 561
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 5 cut(s) 220, 340, 379, 487, 1039
ApoI RAATTY 3 cut(s) 347, 404, 821
AseI ATTAAT 1 cut(s) 1133
AspLEI GCGC 3 cut(s) 220, 379, 819
AspS9I GGNCC 4 cut(s) 765, 875, 898, 937
AsuHPI GGTGA 2 cut(s) 172, 614
AvaII GGWCC 4 cut(s) 765, 875, 898, 937
BaeGI GKGCMC 1 cut(s) 736
BbsI GAAGAC 1 cut(s) 587
Bbv12I GWGCWC 1 cut(s) 736
BbvCI CCTCAGC 1 cut(s) 558
BbvI GCAGC 5 cut(s) 207, 327, 366, 474, 1026
BccI CCATC 2 cut(s) 92, 845
BcgI CGANNNNNNTGC 4 cut(s) 808, 818, 842, 852
BciT130I CCWGG 5 cut(s) 35, 565, 600, 677, 921
BcoDI GTCTC 4 cut(s) 419, 588, 634, 1052
BfaI CTAG 2 cut(s) 644, 975
BfmI CTRYAG 2 cut(s) 380, 488
BfoI RGCGCY 2 cut(s) 221, 380
BglI GCCNNNNNGGC 1 cut(s) 460
BisI GCNGC 6 cut(s) 22, 221, 341, 380, 488, 1040
BlsI GCNGC 6 cut(s) 23, 222, 342, 381, 489, 1041
BmcAI AGTACT 1 cut(s) 1101
Bme1390I CCNGG 5 cut(s) 35, 565, 600, 677, 921
Bme18I GGWCC 4 cut(s) 765, 875, 898, 937
BmgT120I GGNCC 4 cut(s) 765, 875, 898, 937
BmiI GGNNCC 1 cut(s) 877
BmrFI CCNGG 5 cut(s) 35, 565, 600, 677, 921
BmsI GCATC 2 cut(s) 153, 736
BpiI GAAGAC 1 cut(s) 587
Bpu10I CCTNAGC 1 cut(s) 558
BsaBI GATNNNNATC 1 cut(s) 801
BsaHI GRCGYC 1 cut(s) 521
BsaI GGTCTC 1 cut(s) 588
BsaJI CCNNGG 1 cut(s) 598
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 559, 682
Bse1I ACTGG 3 cut(s) 187, 767, 962
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 5 cut(s) 35, 565, 600, 677, 921
BseDI CCNNGG 1 cut(s) 598
BseGI GGATG 2 cut(s) 751, 805
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 3 cut(s) 127, 559, 682
BseMII CTCAG 5 cut(s) 170, 443, 549, 564, 632
BseNI ACTGG 3 cut(s) 187, 767, 962
BseRI GAGGAG 2 cut(s) 845, 1070
BseSI GKGCMC 1 cut(s) 736
BseXI GCAGC 5 cut(s) 207, 327, 366, 474, 1026
BseYI CCCAGC 1 cut(s) 553
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 498, 1034
Bsh1285I CGRYCG 1 cut(s) 729
BshFI GGCC 1 cut(s) 563
BsiEI CGRYCG 1 cut(s) 729
BsiHKAI GWGCWC 1 cut(s) 736
BslI CCNNNNNNNGG 3 cut(s) 127, 559, 682
BsmAI GTCTC 4 cut(s) 419, 588, 634, 1052
BsnI GGCC 1 cut(s) 563
Bso31I GGTCTC 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 736
Bsp143I GATC 3 cut(s) 493, 726, 1141
BspACI CCGC 4 cut(s) 22, 485, 498, 749
BspANI GGCC 1 cut(s) 563
BspCNI CTCAG 5 cut(s) 169, 442, 550, 565, 631
BspFNI CGCG 2 cut(s) 498, 1034
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 877
BspMAI CTGCAG 2 cut(s) 384, 492
BspPI GGATC 1 cut(s) 501
BspTNI GGTCTC 1 cut(s) 588
BsrI ACTGG 3 cut(s) 187, 767, 962
BssECI CCNNGG 1 cut(s) 598
BssMI GATC 3 cut(s) 493, 726, 1141
BssNI GRCGYC 1 cut(s) 521
Bst2UI CCWGG 5 cut(s) 35, 565, 600, 677, 921
Bst4CI ACNGT 2 cut(s) 61, 937
Bst6I CTCTTC 1 cut(s) 816
BstACI GRCGYC 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 7 cut(s) 29, 156, 429, 558, 573, 618, 1049
BstF5I GGATG 2 cut(s) 751, 805
BstFNI CGCG 2 cut(s) 498, 1034
BstH2I RGCGCY 2 cut(s) 221, 380
BstHHI GCGC 3 cut(s) 220, 379, 819
BstKTI GATC 3 cut(s) 496, 729, 1144
BstMAI GTCTC 4 cut(s) 419, 588, 634, 1052
BstMBI GATC 3 cut(s) 493, 726, 1141
BstMCI CGRYCG 1 cut(s) 729
BstMWI GCNNNNNNNGC 5 cut(s) 30, 229, 460, 569, 1045
BstNI CCWGG 5 cut(s) 35, 565, 600, 677, 921
BstSCI CCNGG 5 cut(s) 33, 563, 598, 675, 919
BstSFI CTRYAG 2 cut(s) 380, 488
BstSLI GKGCMC 1 cut(s) 736
BstUI CGCG 2 cut(s) 498, 1034
BstV1I GCAGC 5 cut(s) 207, 327, 366, 474, 1026
BstV2I GAAGAC 1 cut(s) 587
BstXI CCANNNNNNTGG 1 cut(s) 969
BsuRI GGCC 1 cut(s) 563
BtsCI GGATG 2 cut(s) 751, 805
BtsI GCAGTG 2 cut(s) 789, 1144
BtsIMutI CAGTG 5 cut(s) 57, 303, 789, 969, 1144
Cac8I GCNNGC 1 cut(s) 456
CaiI CAGNNNCTG 1 cut(s) 625
CciI TCATGA 1 cut(s) 438
CfoI GCGC 3 cut(s) 220, 379, 819
Cfr13I GGNCC 4 cut(s) 765, 875, 898, 937
CseI GACGC 2 cut(s) 529, 1138
Csp6I GTAC 3 cut(s) 673, 1070, 1100
CviAII CATG 5 cut(s) 389, 439, 444, 512, 1010
CviQI GTAC 3 cut(s) 673, 1070, 1100
DdeI CTNAG 7 cut(s) 29, 156, 429, 558, 573, 618, 1049
DpnI GATC 3 cut(s) 495, 728, 1143
DpnII GATC 3 cut(s) 493, 726, 1141
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
Eco147I AGGCCT 1 cut(s) 563
Eco31I GGTCTC 1 cut(s) 588
Eco32I GATATC 1 cut(s) 450
Eco47I GGWCC 4 cut(s) 765, 875, 898, 937
Eco47III AGCGCT 1 cut(s) 219
Eco57I CTGAAG 2 cut(s) 327, 1028
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 5 cut(s) 33, 563, 598, 675, 919
EcoRV GATATC 1 cut(s) 450
FaeI CATG 5 cut(s) 392, 442, 447, 515, 1013
FatI CATG 5 cut(s) 388, 438, 443, 511, 1009
FauI CCCGC 1 cut(s) 491
Fnu4HI GCNGC 6 cut(s) 22, 221, 341, 380, 488, 1040
FokI GGATG 2 cut(s) 758, 792
Fsp4HI GCNGC 6 cut(s) 22, 221, 341, 380, 488, 1040
FspBI CTAG 2 cut(s) 644, 975
FspI TGCGCA 1 cut(s) 818
GlaI GCGC 3 cut(s) 219, 378, 818
GluI GCNGC 6 cut(s) 22, 221, 341, 380, 488, 1040
GsaI CCCAGC 1 cut(s) 557
HaeII RGCGCY 2 cut(s) 221, 380
HaeIII GGCC 1 cut(s) 563
HgaI GACGC 2 cut(s) 529, 1138
HhaI GCGC 3 cut(s) 220, 379, 819
Hin1I GRCGYC 1 cut(s) 521
Hin1II CATG 5 cut(s) 392, 442, 447, 515, 1013
Hin6I GCGC 3 cut(s) 218, 377, 817
HinP1I GCGC 3 cut(s) 218, 377, 817
HinfI GANTC 8 cut(s) 12, 116, 169, 318, 362, 427, 607, 802
HphI GGTGA 2 cut(s) 172, 614
Hpy166II GTNNAC 3 cut(s) 673, 734, 1072
Hpy188I TCNGA 4 cut(s) 202, 367, 621, 1141
Hpy188III TCNNGA 5 cut(s) 401, 439, 544, 697, 891
Hpy8I GTNNAC 3 cut(s) 673, 734, 1072
HpyAV CCTTC 2 cut(s) 367, 1106
HpyCH4III ACNGT 2 cut(s) 61, 937
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 229, 460, 569, 1045
HpyF3I CTNAG 7 cut(s) 29, 156, 429, 558, 573, 618, 1049
Hsp92I GRCGYC 1 cut(s) 521
Hsp92II CATG 5 cut(s) 392, 442, 447, 515, 1013
HspAI GCGC 3 cut(s) 218, 377, 817
Kzo9I GATC 3 cut(s) 493, 726, 1141
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 5 cut(s) 207, 327, 366, 474, 1026
LweI GCATC 2 cut(s) 153, 736
MaeI CTAG 2 cut(s) 644, 975
MaeIII GTNAC 4 cut(s) 301, 467, 517, 866
MalI GATC 3 cut(s) 495, 728, 1143
MboI GATC 3 cut(s) 493, 726, 1141
MboII GAAGA 9 cut(s) 43, 73, 281, 320, 323, 427, 592, 803, 1121
MhlI GDGCHC 1 cut(s) 736
MluCI AATT 5 cut(s) 347, 404, 631, 821, 978
MlyI GAGTC 2 cut(s) 163, 421
MnlI CCTC 9 cut(s) 453, 458, 553, 659, 711, 819, 823, 954, 1048
MseI TTAA 4 cut(s) 255, 993, 1133, 1177
MslI CAYNNNNRTG 2 cut(s) 420, 695
MspA1I CMGCKG 3 cut(s) 324, 487, 557
MspR9I CCNGG 5 cut(s) 35, 565, 600, 677, 921
MvaI CCWGG 5 cut(s) 35, 565, 600, 677, 921
MvnI CGCG 2 cut(s) 498, 1034
MwoI GCNNNNNNNGC 5 cut(s) 30, 229, 460, 569, 1045
NdeII GATC 3 cut(s) 493, 726, 1141
NlaIII CATG 5 cut(s) 392, 442, 447, 515, 1013
NlaIV GGNNCC 1 cut(s) 877
NmuCI GTSAC 3 cut(s) 301, 517, 866
NsbI TGCGCA 1 cut(s) 818
PagI TCATGA 1 cut(s) 438
PceI AGGCCT 1 cut(s) 563
PfeI GAWTC 6 cut(s) 12, 116, 318, 362, 607, 802
PkrI GCNGC 6 cut(s) 23, 222, 342, 381, 489, 1041
Ple19I CGATCG 1 cut(s) 729
PleI GAGTC 2 cut(s) 163, 421
PpsI GAGTC 2 cut(s) 163, 421
PshBI ATTAAT 1 cut(s) 1133
Psp6I CCWGG 5 cut(s) 33, 563, 598, 675, 919
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 5 cut(s) 33, 563, 598, 675, 919
PspN4I GGNNCC 1 cut(s) 877
PspPI GGNCC 4 cut(s) 765, 875, 898, 937
PstI CTGCAG 2 cut(s) 384, 492
PstNI CAGNNNCTG 1 cut(s) 625
PvuI CGATCG 1 cut(s) 729
PvuII CAGCTG 2 cut(s) 324, 557
RsaI GTAC 3 cut(s) 674, 1071, 1101
RsaNI GTAC 3 cut(s) 673, 1070, 1100
RseI CAYNNNNRTG 2 cut(s) 420, 695
SaqAI TTAA 4 cut(s) 255, 993, 1133, 1177
SatI GCNGC 6 cut(s) 22, 221, 341, 380, 488, 1040
Sau3AI GATC 3 cut(s) 493, 726, 1141
Sau96I GGNCC 4 cut(s) 765, 875, 898, 937
ScaI AGTACT 1 cut(s) 1101
SchI GAGTC 2 cut(s) 163, 421
ScrFI CCNGG 5 cut(s) 35, 565, 600, 677, 921
SduI GDGCHC 1 cut(s) 736
SfaNI GCATC 2 cut(s) 153, 736
SfcI CTRYAG 2 cut(s) 380, 488
SinI GGWCC 4 cut(s) 765, 875, 898, 937
SmiMI CAYNNNNRTG 2 cut(s) 420, 695
Sse9I AATT 5 cut(s) 347, 404, 631, 821, 978
SseBI AGGCCT 1 cut(s) 563
SsiI CCGC 4 cut(s) 22, 485, 498, 749
SspMI CTAG 2 cut(s) 644, 975
StuI AGGCCT 1 cut(s) 563
StyD4I CCNGG 5 cut(s) 33, 563, 598, 675, 919
TaaI ACNGT 2 cut(s) 61, 937
TaqI TCGA 6 cut(s) 15, 180, 725, 755, 828, 1107
TasI AATT 5 cut(s) 347, 404, 631, 821, 978
TatI WGTACW 2 cut(s) 1069, 1099
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 6 cut(s) 12, 116, 318, 362, 607, 802
Tru1I TTAA 4 cut(s) 255, 993, 1133, 1177
Tru9I TTAA 4 cut(s) 255, 993, 1133, 1177
TscAI CASTG 5 cut(s) 64, 310, 789, 969, 1151
TseFI GTSAC 3 cut(s) 301, 517, 866
TseI GCWGC 5 cut(s) 220, 340, 379, 487, 1039
Tsp45I GTSAC 3 cut(s) 301, 517, 866
TspDTI ATGAA 9 cut(s) 73, 181, 377, 427, 500, 593, 679, 814, 815
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 5 cut(s) 64, 310, 789, 969, 1151
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 4 cut(s) 765, 875, 898, 937
VspI ATTAAT 1 cut(s) 1133
XapI RAATTY 3 cut(s) 347, 404, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 561
XspI CTAG 2 cut(s) 644, 975
ZrmI AGTACT 1 cut(s) 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.