Rroxscaffold_6G00416520

Lactosylceramide 4-alpha-galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013878
Physical Location & Seq
Reverse (-)
38526483 .. 38527751
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00416520.1

Sequence Viewer

Length: 1269 bp
ATGATGAGTAGCCGAATCGACAACCGCCTACTTAGCCTGGCGAAGTTCCACATTTTCTGCACTGTCTCACTTGTTGCAATCATCTTCATCATCTATGCAGATGGTATCATATATACTGATTCCCAAAACTCGGTTGAGTTGAAAGATAAAGCTGTTTCTCAGGTGCTCCAGACTCATAGTATCGAACGCCAGTTCATCAAGTCTGATATAACTCGTAAAGCGCTGCGTTCTATGCAAGAAAATGTCCTTGATGTCGTTGAAGTTGATACAGAAGATACAAGTCCTGTTGTTGCTCCTTTCAATGTCACTGAAGAAGAAAGAATCAGCTGGTTTCGGGGAAAGCTGCCCGAATTTGTGATTTTTGATTCGGACGAGAAGGCGCTGCAGTTTCATGGTAGAGTTCTTGAATTCTTTGACCATAAGTGTGAGACTCAGTTCTTCATGACATGGATATCGCCTGCGAGGTCTTTTGGAAGCAGAGAATTCCTGTCGATGGAGAGTGTATTCAGAGCTCACCCTCGTGGTTGCTTGATGATTCTTTCGAGAACCATGGACTCCAGGCGAGGTTACAGGCTTCTGAAACCGCTGCAGGATCGCGGGTTCAAAGTTCATGCTGTGACGCCAGACTTGTCATTTCTGTTCAAGAACACCCCAGCTGAGGCCTGGTTTGCTGAGATGAAGACAGGCAACAGAGACCCTGGTGAGATTCCATTAGCTCAGAACCTGTCTAATTTATTGAGACTAGCAGTTTTATACAAGTATGGAGGTGTGTACCTGGACACGGATTTCATTGTCATGAAATCATTTTCGGGGTTGAGGAACTCGATCGGTGCACAAAGCATGGATGCGGTGTCGAAAAACTGGACCAGACTGAACAATGCAGTGCTGGTGTTTGATATGAATCATCCTCTTCTGCGCAAATTCATCGAGGAGTTTGCCTTGACTTTTGATGGGAATAAATGGGGTCACAATGGACCTTATCTTGTTTCCAGAGTGGTCCAAAGAGTGCAAAACAGACCTGGATATAACTTTACAGTCCTGCCTCCTATGGCTTTCTATCCAGTGGATTGGACTAGAATTGGGGGCTTGTTTAAGAGGCCGAAAAACCATGCTGACACTAAATGGATAAACGCGAAGCTGCTTCAGCTTAGTGAGGAGACTTATGGAGTACACCTATGGAACAAGCAAAGTAGCAGAGTACTAATCGAAGAAGGAAGCATTATGCAGAGATTAATCTCCGATCACTGCATCATCTGTAAAGACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

422

Amino Acids

48.63

Weight (kDa)

8.82

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 160 - 280 3.5e-22 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 298 - 420 6.1e-30 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38152
fragaria_vesca FvH4_6g11720
malus_domestica MD04G1149800.v1.1 MD12G1164200.v1.1
prunus_persica Prupe.6G274700_v2.0.a1
pyrus_communis pycom04g13530
rosa_chinensis RchiOBHm_Chr3g0463491
rosa_laevigata RLG00000019555 RLG00000024783
rosa_multiflora Rmu_sc0000923.1_g000020
rosa_roxburghii Rroxscaffold_6G00416520
rosa_rugosa Rorug03G0065100
rosa_samantha Rh3AG122300 Rh3BG127000 Rh3CG128900 Rh3DG127800
rosa_wichuraiana Rw0G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 917
AccII CGCG 2 cut(s) 597, 1133
AciI CCGC 4 cut(s) 25, 584, 597, 848
AclWI GGATC 1 cut(s) 600
AcsI RAATTY 4 cut(s) 350, 407, 482, 920
AcuI CTGAAG 2 cut(s) 330, 1127
AcyI GRCGYC 1 cut(s) 620
AfaI GTAC 3 cut(s) 773, 1170, 1200
AfeI AGCGCT 1 cut(s) 222
AfiI CCNNNNNNNGG 4 cut(s) 130, 493, 658, 781
AgsI TTSAA 6 cut(s) 142, 260, 301, 407, 604, 643
AjnI CCWGG 6 cut(s) 36, 557, 662, 697, 774, 1018
AleI CACNNNNGTG 1 cut(s) 519
AloI GAACNNNNNNTCC 2 cut(s) 584, 616
AluBI AGCT 8 cut(s) 152, 327, 343, 512, 656, 716, 1138, 1147
AluI AGCT 8 cut(s) 152, 327, 343, 512, 656, 716, 1138, 1147
Alw21I GWGCWC 3 cut(s) 168, 514, 835
Alw26I GTCTC 5 cut(s) 70, 422, 687, 733, 1151
Alw44I GTGCAC 1 cut(s) 831
AlwI GGATC 1 cut(s) 600
AlwNI CAGNNNCTG 1 cut(s) 724
Aor51HI AGCGCT 1 cut(s) 222
AoxI GGCC 2 cut(s) 660, 1097
ApaLI GTGCAC 1 cut(s) 831
ApeKI GCWGC 5 cut(s) 223, 343, 382, 586, 1138
ApoI RAATTY 4 cut(s) 350, 407, 482, 920
AseI ATTAAT 1 cut(s) 1232
AspLEI GCGC 3 cut(s) 223, 382, 918
AspS9I GGNCC 3 cut(s) 864, 974, 997
AsuHPI GGTGA 2 cut(s) 506, 713
AvaII GGWCC 3 cut(s) 864, 974, 997
BaeGI GKGCMC 1 cut(s) 835
BanII GRGCYC 1 cut(s) 514
BauI CACGAG 1 cut(s) 519
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 3 cut(s) 168, 514, 835
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 5 cut(s) 210, 330, 369, 573, 1125
BccI CCATC 3 cut(s) 95, 487, 944
BcgI CGANNNNNNTGC 4 cut(s) 907, 917, 941, 951
BciT130I CCWGG 6 cut(s) 38, 559, 664, 699, 776, 1020
BcoDI GTCTC 5 cut(s) 70, 422, 687, 733, 1151
BfaI CTAG 2 cut(s) 743, 1074
BfmI CTRYAG 2 cut(s) 383, 587
BfoI RGCGCY 2 cut(s) 224, 383
BisI GCNGC 5 cut(s) 224, 344, 383, 587, 1139
BlsI GCNGC 5 cut(s) 225, 345, 384, 588, 1140
BmcAI AGTACT 1 cut(s) 1200
Bme1390I CCNGG 6 cut(s) 38, 559, 664, 699, 776, 1020
Bme18I GGWCC 3 cut(s) 864, 974, 997
BmgT120I GGNCC 3 cut(s) 864, 974, 997
BmrFI CCNGG 6 cut(s) 38, 559, 664, 699, 776, 1020
BmsI GCATC 2 cut(s) 835, 1257
BpiI GAAGAC 1 cut(s) 686
BpmI CTGGAG 2 cut(s) 152, 541
Bpu10I CCTNAGC 1 cut(s) 657
BsaBI GATNNNNATC 1 cut(s) 900
BsaHI GRCGYC 1 cut(s) 620
BsaI GGTCTC 1 cut(s) 687
BsaJI CCNNGG 2 cut(s) 549, 697
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 493, 658, 781
Bse1I ACTGG 3 cut(s) 190, 866, 1061
Bse8I GATNNNNATC 1 cut(s) 900
BseBI CCWGG 6 cut(s) 38, 559, 664, 699, 776, 1020
BseDI CCNNGG 2 cut(s) 549, 697
BseGI GGATG 2 cut(s) 850, 904
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 4 cut(s) 130, 493, 658, 781
BseMII CTCAG 5 cut(s) 173, 446, 648, 663, 731
BseNI ACTGG 3 cut(s) 190, 866, 1061
BseRI GAGGAG 2 cut(s) 944, 1169
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 5 cut(s) 210, 330, 369, 573, 1125
BseYI CCCAGC 1 cut(s) 652
BsgI GTGCAG 1 cut(s) 43
Bsh1236I CGCG 2 cut(s) 597, 1133
Bsh1285I CGRYCG 1 cut(s) 828
BshFI GGCC 2 cut(s) 662, 1099
BsiEI CGRYCG 1 cut(s) 828
BsiHKAI GWGCWC 3 cut(s) 168, 514, 835
BslI CCNNNNNNNGG 4 cut(s) 130, 493, 658, 781
BsmAI GTCTC 5 cut(s) 70, 422, 687, 733, 1151
BsnI GGCC 2 cut(s) 662, 1099
Bso31I GGTCTC 1 cut(s) 687
Bsp1286I GDGCHC 3 cut(s) 168, 514, 835
Bsp143I GATC 3 cut(s) 592, 825, 1240
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 4 cut(s) 25, 584, 597, 848
BspANI GGCC 2 cut(s) 662, 1099
BspCNI CTCAG 5 cut(s) 172, 445, 649, 664, 730
BspFNI CGCG 2 cut(s) 597, 1133
BspHI TCATGA 2 cut(s) 441, 795
BspMAI CTGCAG 2 cut(s) 387, 591
BspPI GGATC 1 cut(s) 600
BspTNI GGTCTC 1 cut(s) 687
BsrI ACTGG 3 cut(s) 190, 866, 1061
BssECI CCNNGG 2 cut(s) 549, 697
BssMI GATC 3 cut(s) 592, 825, 1240
BssNI GRCGYC 1 cut(s) 620
BssSI CACGAG 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 549
Bst2BI CACGAG 1 cut(s) 519
Bst2UI CCWGG 6 cut(s) 38, 559, 664, 699, 776, 1020
Bst4CI ACNGT 2 cut(s) 64, 1036
Bst6I CTCTTC 1 cut(s) 915
BstACI GRCGYC 1 cut(s) 620
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 7 cut(s) 32, 159, 432, 657, 672, 717, 1148
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 850, 904
BstFNI CGCG 2 cut(s) 597, 1133
BstH2I RGCGCY 2 cut(s) 224, 383
BstHHI GCGC 3 cut(s) 223, 382, 918
BstKTI GATC 3 cut(s) 595, 828, 1243
BstMAI GTCTC 5 cut(s) 70, 422, 687, 733, 1151
BstMBI GATC 3 cut(s) 592, 825, 1240
BstMCI CGRYCG 1 cut(s) 828
BstMWI GCNNNNNNNGC 4 cut(s) 33, 232, 668, 1144
BstNI CCWGG 6 cut(s) 38, 559, 664, 699, 776, 1020
BstSCI CCNGG 6 cut(s) 36, 557, 662, 697, 774, 1018
BstSFI CTRYAG 2 cut(s) 383, 587
BstSLI GKGCMC 1 cut(s) 835
BstUI CGCG 2 cut(s) 597, 1133
BstV1I GCAGC 5 cut(s) 210, 330, 369, 573, 1125
BstV2I GAAGAC 1 cut(s) 686
BstXI CCANNNNNNTGG 1 cut(s) 1068
BsuRI GGCC 2 cut(s) 662, 1099
BtgI CCRYGG 1 cut(s) 549
BtsCI GGATG 2 cut(s) 850, 904
BtsI GCAGTG 2 cut(s) 888, 1243
BtsIMutI CAGTG 5 cut(s) 60, 306, 888, 1068, 1243
Cac8I GCNNGC 1 cut(s) 459
CaiI CAGNNNCTG 1 cut(s) 724
CciI TCATGA 2 cut(s) 441, 795
CfoI GCGC 3 cut(s) 223, 382, 918
Cfr13I GGNCC 3 cut(s) 864, 974, 997
CseI GACGC 1 cut(s) 628
Csp6I GTAC 3 cut(s) 772, 1169, 1199
CviAII CATG 8 cut(s) 392, 442, 447, 550, 611, 796, 841, 1109
CviQI GTAC 3 cut(s) 772, 1169, 1199
DdeI CTNAG 7 cut(s) 32, 159, 432, 657, 672, 717, 1148
DpnI GATC 3 cut(s) 594, 827, 1242
DpnII GATC 3 cut(s) 592, 825, 1240
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
Ecl136II GAGCTC 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 662
Eco24I GRGCYC 1 cut(s) 514
Eco31I GGTCTC 1 cut(s) 687
Eco32I GATATC 1 cut(s) 453
Eco47I GGWCC 3 cut(s) 864, 974, 997
Eco47III AGCGCT 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 512
Eco57I CTGAAG 2 cut(s) 330, 1127
EcoICRI GAGCTC 1 cut(s) 512
EcoRI GAATTC 2 cut(s) 407, 482
EcoRII CCWGG 6 cut(s) 36, 557, 662, 697, 774, 1018
EcoRV GATATC 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 549
EcoT38I GRGCYC 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 8 cut(s) 395, 445, 450, 553, 614, 799, 844, 1112
FatI CATG 8 cut(s) 391, 441, 446, 549, 610, 795, 840, 1108
FauI CCCGC 1 cut(s) 590
Fnu4HI GCNGC 5 cut(s) 224, 344, 383, 587, 1139
FokI GGATG 2 cut(s) 857, 891
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 5 cut(s) 224, 344, 383, 587, 1139
FspBI CTAG 2 cut(s) 743, 1074
FspI TGCGCA 1 cut(s) 917
GlaI GCGC 3 cut(s) 222, 381, 917
GluI GCNGC 5 cut(s) 224, 344, 383, 587, 1139
GsaI CCCAGC 1 cut(s) 656
GsuI CTGGAG 2 cut(s) 152, 541
HaeII RGCGCY 2 cut(s) 224, 383
HaeIII GGCC 2 cut(s) 662, 1099
HgaI GACGC 1 cut(s) 628
HhaI GCGC 3 cut(s) 223, 382, 918
Hin1I GRCGYC 1 cut(s) 620
Hin1II CATG 8 cut(s) 395, 445, 450, 553, 614, 799, 844, 1112
Hin6I GCGC 3 cut(s) 221, 380, 916
HinP1I GCGC 3 cut(s) 221, 380, 916
HphI GGTGA 2 cut(s) 506, 713
Hpy166II GTNNAC 3 cut(s) 772, 833, 1171
Hpy188I TCNGA 6 cut(s) 205, 370, 509, 579, 720, 1240
Hpy188III TCNNGA 7 cut(s) 169, 404, 442, 543, 643, 796, 990
Hpy8I GTNNAC 3 cut(s) 772, 833, 1171
HpyAV CCTTC 2 cut(s) 370, 1205
HpyCH4III ACNGT 2 cut(s) 64, 1036
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 232, 668, 1144
HpyF3I CTNAG 7 cut(s) 32, 159, 432, 657, 672, 717, 1148
Hsp92I GRCGYC 1 cut(s) 620
Hsp92II CATG 8 cut(s) 395, 445, 450, 553, 614, 799, 844, 1112
HspAI GCGC 3 cut(s) 221, 380, 916
Kzo9I GATC 3 cut(s) 592, 825, 1240
LmnI GCTCC 2 cut(s) 171, 298
Lsp1109I GCAGC 5 cut(s) 210, 330, 369, 573, 1125
LweI GCATC 2 cut(s) 835, 1257
MaeI CTAG 2 cut(s) 743, 1074
MaeIII GTNAC 4 cut(s) 304, 566, 616, 965
MalI GATC 3 cut(s) 594, 827, 1242
MboI GATC 3 cut(s) 592, 825, 1240
MboII GAAGA 8 cut(s) 76, 284, 323, 326, 430, 691, 902, 1220
MhlI GDGCHC 3 cut(s) 168, 514, 835
MluCI AATT 6 cut(s) 350, 407, 482, 730, 920, 1077
MlyI GAGTC 3 cut(s) 166, 424, 548
MseI TTAA 2 cut(s) 1092, 1232
MslI CAYNNNNRTG 3 cut(s) 423, 519, 794
MspA1I CMGCKG 3 cut(s) 327, 586, 656
MspR9I CCNGG 6 cut(s) 38, 559, 664, 699, 776, 1020
MvaI CCWGG 6 cut(s) 38, 559, 664, 699, 776, 1020
MvnI CGCG 2 cut(s) 597, 1133
MwoI GCNNNNNNNGC 4 cut(s) 33, 232, 668, 1144
NcoI CCATGG 1 cut(s) 549
NdeII GATC 3 cut(s) 592, 825, 1240
NlaIII CATG 8 cut(s) 395, 445, 450, 553, 614, 799, 844, 1112
NmuCI GTSAC 3 cut(s) 304, 616, 965
NsbI TGCGCA 1 cut(s) 917
OliI CACNNNNGTG 1 cut(s) 519
PagI TCATGA 2 cut(s) 441, 795
PceI AGGCCT 1 cut(s) 662
PfeI GAWTC 7 cut(s) 15, 119, 321, 365, 535, 706, 901
PkrI GCNGC 5 cut(s) 225, 345, 384, 588, 1140
Ple19I CGATCG 1 cut(s) 828
PleI GAGTC 3 cut(s) 166, 424, 548
PpsI GAGTC 3 cut(s) 166, 424, 548
PshBI ATTAAT 1 cut(s) 1232
Psp124BI GAGCTC 1 cut(s) 514
Psp6I CCWGG 6 cut(s) 36, 557, 662, 697, 774, 1018
PspFI CCCAGC 1 cut(s) 652
PspGI CCWGG 6 cut(s) 36, 557, 662, 697, 774, 1018
PspPI GGNCC 3 cut(s) 864, 974, 997
PstI CTGCAG 2 cut(s) 387, 591
PstNI CAGNNNCTG 1 cut(s) 724
PvuI CGATCG 1 cut(s) 828
PvuII CAGCTG 2 cut(s) 327, 656
RsaI GTAC 3 cut(s) 773, 1170, 1200
RsaNI GTAC 3 cut(s) 772, 1169, 1199
RseI CAYNNNNRTG 3 cut(s) 423, 519, 794
SacI GAGCTC 1 cut(s) 514
SaqAI TTAA 2 cut(s) 1092, 1232
SatI GCNGC 5 cut(s) 224, 344, 383, 587, 1139
Sau3AI GATC 3 cut(s) 592, 825, 1240
Sau96I GGNCC 3 cut(s) 864, 974, 997
ScaI AGTACT 1 cut(s) 1200
SchI GAGTC 3 cut(s) 166, 424, 548
ScrFI CCNGG 6 cut(s) 38, 559, 664, 699, 776, 1020
SduI GDGCHC 3 cut(s) 168, 514, 835
SfaNI GCATC 2 cut(s) 835, 1257
SfcI CTRYAG 2 cut(s) 383, 587
SinI GGWCC 3 cut(s) 864, 974, 997
SmiMI CAYNNNNRTG 3 cut(s) 423, 519, 794
Sse9I AATT 6 cut(s) 350, 407, 482, 730, 920, 1077
SseBI AGGCCT 1 cut(s) 662
SsiI CCGC 4 cut(s) 25, 584, 597, 848
SspMI CTAG 2 cut(s) 743, 1074
SstI GAGCTC 1 cut(s) 514
StuI AGGCCT 1 cut(s) 662
StyD4I CCNGG 6 cut(s) 36, 557, 662, 697, 774, 1018
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 2 cut(s) 64, 1036
TaqI TCGA 8 cut(s) 18, 183, 491, 542, 824, 854, 927, 1206
TasI AATT 6 cut(s) 350, 407, 482, 730, 920, 1077
TatI WGTACW 2 cut(s) 1168, 1198
TfiI GAWTC 7 cut(s) 15, 119, 321, 365, 535, 706, 901
Tru1I TTAA 2 cut(s) 1092, 1232
Tru9I TTAA 2 cut(s) 1092, 1232
TscAI CASTG 5 cut(s) 67, 313, 888, 1068, 1250
TseFI GTSAC 3 cut(s) 304, 616, 965
TseI GCWGC 5 cut(s) 223, 343, 382, 586, 1138
Tsp45I GTSAC 3 cut(s) 304, 616, 965
TspGWI ACGGA 1 cut(s) 797
TspRI CASTG 5 cut(s) 67, 313, 888, 1068, 1250
VneI GTGCAC 1 cut(s) 831
VpaK11BI GGWCC 3 cut(s) 864, 974, 997
VspI ATTAAT 1 cut(s) 1232
XapI RAATTY 4 cut(s) 350, 407, 482, 920
XcmI CCANNNNNNNNNTGG 1 cut(s) 660
XspI CTAG 2 cut(s) 743, 1074
ZrmI AGTACT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.