Rh3BG127000

Lactosylceramide 4-alpha-galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0013878
Physical Location & Seq
Forward (+)
10518646 .. 10519920
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG127000.1

Sequence Viewer

Length: 1275 bp
ATGAGTAGCCGAATCGACAGCCGCCTACTTAGCCTGGCGAAGTTCCACATCTTCTGCACTGTATCACTTGTTGCAATCATCTTCATCATTTATGCAGATGGTATCATATATACTGATTCCCAAAACTCGGTTGAGTTGAAAGATAAAGCTGTTTCTCAGGTGCTGCAGACTCATAGTATCGAACGCCAGTTCATCAAGTCTGATATAACTCGTAAAGCGCTGCGTTCTATGCAAGAAAATGTCCTTGATGTCGTTGAAGTTGATACAGAAGATACAAGTCCTGTTGTTGCTCCTTTCAATGTCACTGAAGAAGAAAGAATCAGCTGGTTTCGGGAAAAGCTGCCCGAATTTGTGATTTTTGATTCAGACGAGAAGGCGCTGCAGTTTCATGGTAGAGTTCTTGAATTCTTTGACCATAAGTGTGATACTCAGTTCTTCATGACATGGATATCGCCTGCGAGGTCTTTTGGAAGCAGAGAATTCCTGTCGATGGAGAGTGTATTCAGAGCTCACCCTCGTGGTTGCTTGATGATTCTTTCGAGAACCATGGACTCCAGGCGAGGTTACAGGCTTCTGAAACCGCTGCAGGATCGCGGGTTCAAAGTTCATGCTGTGACGCCAGACTTGTCATTTCTGTTCAAGAACACCCCAGCTGACGCCTGGTTTGCTGAGATGAAGACAGGCAACAGAGACCCTGGTGAGATTCCATTAGCTCAGAACCTGTCTAATTTATTGAGACTTGCAGTTTTATACAAGTATGGAGGTGTGTACCTGGACACGGATTTCATTGTCATGAAATCATTTTCGGGGTTGAGGAACTCAATCGGTGCACAAAGTATGGATGCAGTGTCGAAAAACTGGACCAGACTGAACAATGCAGTGCTGGTGTTTGATATGAATCATCCTCTTCTGCGCAAATTCATCGAGGAGTTTGCCTTGACTTTTGATGGGAATAAATGGGGTCACAATGGACCCTATCTTGTTTCCAGAGTGGTCCAAAGAGTGCAAAACAGACCTGGATATAACTTTACAGTCCTGCCTCGTATGGCTTTCTATCCAGTGGATTGGTCTAGAATTGGGGGCTTGTTTAAGAAACCGAAAAACCATGCTGACACTAAATGGATAAACGCGAAGCTGGTTCAGCTTAGTGAGGAGACTTATGGAGTACACCTATGGAACAAGCAAAGTAGCAGAGTACTAATCGAAGAAGGAAGCATTATGCAGAGATTAATCTCCGATCACTGCGTCATCTGTAAAGACATATATAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

424

Amino Acids

48.84

Weight (kDa)

8.8

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 159 - 279 3.6e-22 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 297 - 419 9.7e-30 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38152
fragaria_vesca FvH4_6g11720
malus_domestica MD04G1149800.v1.1 MD12G1164200.v1.1
prunus_persica Prupe.6G274700_v2.0.a1
pyrus_communis pycom04g13530
rosa_chinensis RchiOBHm_Chr3g0463491
rosa_laevigata RLG00000019555 RLG00000024783
rosa_multiflora Rmu_sc0000923.1_g000020
rosa_roxburghii Rroxscaffold_6G00416520
rosa_rugosa Rorug03G0065100
rosa_samantha Rh3AG122300 Rh3BG127000 Rh3CG128900 Rh3DG127800
rosa_wichuraiana Rw0G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 914
AccII CGCG 2 cut(s) 594, 1130
AciI CCGC 3 cut(s) 22, 581, 594
AclWI GGATC 1 cut(s) 597
AcsI RAATTY 4 cut(s) 347, 404, 479, 917
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 2 cut(s) 617, 657
AfaI GTAC 3 cut(s) 770, 1167, 1197
AfeI AGCGCT 1 cut(s) 219
AfiI CCNNNNNNNGG 3 cut(s) 127, 490, 778
AgsI TTSAA 6 cut(s) 139, 257, 298, 404, 601, 640
AjnI CCWGG 6 cut(s) 33, 554, 659, 694, 771, 1015
AleI CACNNNNGTG 1 cut(s) 516
AloI GAACNNNNNNTCC 2 cut(s) 581, 613
AluBI AGCT 8 cut(s) 149, 324, 340, 509, 653, 713, 1135, 1144
AluI AGCT 8 cut(s) 149, 324, 340, 509, 653, 713, 1135, 1144
Alw21I GWGCWC 2 cut(s) 511, 832
Alw26I GTCTC 3 cut(s) 684, 730, 1148
Alw44I GTGCAC 1 cut(s) 828
AlwI GGATC 1 cut(s) 597
AlwNI CAGNNNCTG 2 cut(s) 163, 721
Aor51HI AGCGCT 1 cut(s) 219
ApaLI GTGCAC 1 cut(s) 828
ApeKI GCWGC 5 cut(s) 163, 220, 340, 379, 583
ApoI RAATTY 4 cut(s) 347, 404, 479, 917
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AseI ATTAAT 1 cut(s) 1229
AspLEI GCGC 3 cut(s) 220, 379, 915
AspS9I GGNCC 3 cut(s) 861, 971, 994
AsuHPI GGTGA 2 cut(s) 503, 710
AvaII GGWCC 3 cut(s) 861, 971, 994
BaeGI GKGCMC 1 cut(s) 832
BanII GRGCYC 1 cut(s) 511
BauI CACGAG 1 cut(s) 516
BbsI GAAGAC 1 cut(s) 683
Bbv12I GWGCWC 2 cut(s) 511, 832
BbvI GCAGC 5 cut(s) 150, 207, 327, 366, 570
BccI CCATC 3 cut(s) 92, 484, 941
BcgI CGANNNNNNTGC 4 cut(s) 904, 914, 938, 948
BciT130I CCWGG 6 cut(s) 35, 556, 661, 696, 773, 1017
BcoDI GTCTC 3 cut(s) 684, 730, 1148
BfaI CTAG 1 cut(s) 1071
BfmI CTRYAG 3 cut(s) 164, 380, 584
BfoI RGCGCY 2 cut(s) 221, 380
BisI GCNGC 6 cut(s) 22, 164, 221, 341, 380, 584
BlsI GCNGC 6 cut(s) 23, 165, 222, 342, 381, 585
BmcAI AGTACT 1 cut(s) 1197
Bme1390I CCNGG 6 cut(s) 35, 556, 661, 696, 773, 1017
Bme18I GGWCC 3 cut(s) 861, 971, 994
BmgT120I GGNCC 3 cut(s) 861, 971, 994
BmiI GGNNCC 1 cut(s) 973
BmrFI CCNGG 6 cut(s) 35, 556, 661, 696, 773, 1017
BmsI GCATC 1 cut(s) 832
BpiI GAAGAC 1 cut(s) 683
BpmI CTGGAG 1 cut(s) 538
BsaBI GATNNNNATC 1 cut(s) 897
BsaHI GRCGYC 2 cut(s) 617, 657
BsaI GGTCTC 1 cut(s) 684
BsaJI CCNNGG 2 cut(s) 546, 694
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 490, 778
Bse1I ACTGG 3 cut(s) 187, 863, 1058
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 6 cut(s) 35, 556, 661, 696, 773, 1017
BseDI CCNNGG 2 cut(s) 546, 694
BseGI GGATG 2 cut(s) 847, 901
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 3 cut(s) 127, 490, 778
BseMII CTCAG 4 cut(s) 170, 443, 660, 728
BseNI ACTGG 3 cut(s) 187, 863, 1058
BseRI GAGGAG 2 cut(s) 941, 1166
BseSI GKGCMC 1 cut(s) 832
BseXI GCAGC 5 cut(s) 150, 207, 327, 366, 570
BseYI CCCAGC 1 cut(s) 649
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 594, 1130
BsiHKAI GWGCWC 2 cut(s) 511, 832
BslI CCNNNNNNNGG 3 cut(s) 127, 490, 778
BsmAI GTCTC 3 cut(s) 684, 730, 1148
Bso31I GGTCTC 1 cut(s) 684
Bsp1286I GDGCHC 2 cut(s) 511, 832
Bsp143I GATC 2 cut(s) 589, 1237
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 3 cut(s) 22, 581, 594
BspCNI CTCAG 4 cut(s) 169, 442, 661, 727
BspFNI CGCG 2 cut(s) 594, 1130
BspHI TCATGA 2 cut(s) 438, 792
BspLI GGNNCC 1 cut(s) 973
BspMAI CTGCAG 3 cut(s) 168, 384, 588
BspPI GGATC 1 cut(s) 597
BspTNI GGTCTC 1 cut(s) 684
BsrI ACTGG 3 cut(s) 187, 863, 1058
BssECI CCNNGG 2 cut(s) 546, 694
BssMI GATC 2 cut(s) 589, 1237
BssNI GRCGYC 2 cut(s) 617, 657
BssSI CACGAG 1 cut(s) 516
BssT1I CCWWGG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 516
Bst2UI CCWGG 6 cut(s) 35, 556, 661, 696, 773, 1017
Bst4CI ACNGT 2 cut(s) 61, 1033
Bst6I CTCTTC 1 cut(s) 912
BstACI GRCGYC 2 cut(s) 617, 657
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 6 cut(s) 29, 156, 429, 669, 714, 1145
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 2 cut(s) 847, 901
BstFNI CGCG 2 cut(s) 594, 1130
BstH2I RGCGCY 2 cut(s) 221, 380
BstHHI GCGC 3 cut(s) 220, 379, 915
BstKTI GATC 2 cut(s) 592, 1240
BstMAI GTCTC 3 cut(s) 684, 730, 1148
BstMBI GATC 2 cut(s) 589, 1237
BstMWI GCNNNNNNNGC 4 cut(s) 30, 229, 665, 1141
BstNI CCWGG 6 cut(s) 35, 556, 661, 696, 773, 1017
BstSCI CCNGG 6 cut(s) 33, 554, 659, 694, 771, 1015
BstSFI CTRYAG 3 cut(s) 164, 380, 584
BstSLI GKGCMC 1 cut(s) 832
BstUI CGCG 2 cut(s) 594, 1130
BstV1I GCAGC 5 cut(s) 150, 207, 327, 366, 570
BstV2I GAAGAC 1 cut(s) 683
BstXI CCANNNNNNTGG 1 cut(s) 1065
BtgI CCRYGG 1 cut(s) 546
BtsCI GGATG 2 cut(s) 847, 901
BtsI GCAGTG 3 cut(s) 852, 885, 1240
BtsIMutI CAGTG 6 cut(s) 57, 303, 852, 885, 1065, 1240
Cac8I GCNNGC 1 cut(s) 456
CaiI CAGNNNCTG 2 cut(s) 163, 721
CciI TCATGA 2 cut(s) 438, 792
CfoI GCGC 3 cut(s) 220, 379, 915
Cfr13I GGNCC 3 cut(s) 861, 971, 994
CseI GACGC 3 cut(s) 625, 665, 1234
Csp6I GTAC 3 cut(s) 769, 1166, 1196
CviAII CATG 7 cut(s) 389, 439, 444, 547, 608, 793, 1106
CviQI GTAC 3 cut(s) 769, 1166, 1196
DdeI CTNAG 6 cut(s) 29, 156, 429, 669, 714, 1145
DpnI GATC 2 cut(s) 591, 1239
DpnII GATC 2 cut(s) 589, 1237
Eam1104I CTCTTC 1 cut(s) 912
EarI CTCTTC 1 cut(s) 912
Ecl136II GAGCTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 511
Eco31I GGTCTC 1 cut(s) 684
Eco32I GATATC 1 cut(s) 450
Eco47I GGWCC 3 cut(s) 861, 971, 994
Eco47III AGCGCT 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 509
EcoRI GAATTC 2 cut(s) 404, 479
EcoRII CCWGG 6 cut(s) 33, 554, 659, 694, 771, 1015
EcoRV GATATC 1 cut(s) 450
EcoT14I CCWWGG 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 7 cut(s) 392, 442, 447, 550, 611, 796, 1109
FatI CATG 7 cut(s) 388, 438, 443, 546, 607, 792, 1105
FauI CCCGC 1 cut(s) 587
Fnu4HI GCNGC 6 cut(s) 22, 164, 221, 341, 380, 584
FokI GGATG 2 cut(s) 854, 888
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 6 cut(s) 22, 164, 221, 341, 380, 584
FspBI CTAG 1 cut(s) 1071
FspI TGCGCA 1 cut(s) 914
GlaI GCGC 3 cut(s) 219, 378, 914
GluI GCNGC 6 cut(s) 22, 164, 221, 341, 380, 584
GsaI CCCAGC 1 cut(s) 653
GsuI CTGGAG 1 cut(s) 538
HaeII RGCGCY 2 cut(s) 221, 380
HgaI GACGC 3 cut(s) 625, 665, 1234
HhaI GCGC 3 cut(s) 220, 379, 915
Hin1I GRCGYC 2 cut(s) 617, 657
Hin1II CATG 7 cut(s) 392, 442, 447, 550, 611, 796, 1109
Hin6I GCGC 3 cut(s) 218, 377, 913
HinP1I GCGC 3 cut(s) 218, 377, 913
HinfI GANTC 9 cut(s) 12, 116, 169, 318, 362, 532, 551, 703, 898
HphI GGTGA 2 cut(s) 503, 710
Hpy166II GTNNAC 3 cut(s) 769, 830, 1168
Hpy188I TCNGA 6 cut(s) 202, 367, 506, 576, 717, 1237
Hpy188III TCNNGA 8 cut(s) 332, 401, 439, 540, 640, 793, 987, 1071
Hpy8I GTNNAC 3 cut(s) 769, 830, 1168
HpyAV CCTTC 2 cut(s) 367, 1202
HpyCH4III ACNGT 2 cut(s) 61, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 229, 665, 1141
HpyF3I CTNAG 6 cut(s) 29, 156, 429, 669, 714, 1145
Hsp92I GRCGYC 2 cut(s) 617, 657
Hsp92II CATG 7 cut(s) 392, 442, 447, 550, 611, 796, 1109
HspAI GCGC 3 cut(s) 218, 377, 913
Kzo9I GATC 2 cut(s) 589, 1237
LmnI GCTCC 1 cut(s) 295
Lsp1109I GCAGC 5 cut(s) 150, 207, 327, 366, 570
LweI GCATC 1 cut(s) 832
MaeI CTAG 1 cut(s) 1071
MaeIII GTNAC 4 cut(s) 301, 563, 613, 962
MalI GATC 2 cut(s) 591, 1239
MboI GATC 2 cut(s) 589, 1237
MboII GAAGA 9 cut(s) 43, 73, 281, 320, 323, 427, 688, 899, 1217
MhlI GDGCHC 2 cut(s) 511, 832
MluCI AATT 6 cut(s) 347, 404, 479, 727, 917, 1074
MlyI GAGTC 2 cut(s) 163, 545
MnlI CCTC 9 cut(s) 453, 525, 554, 755, 807, 915, 919, 1050, 1144
MseI TTAA 3 cut(s) 1089, 1229, 1273
MslI CAYNNNNRTG 3 cut(s) 420, 516, 791
MspA1I CMGCKG 3 cut(s) 324, 583, 653
MspR9I CCNGG 6 cut(s) 35, 556, 661, 696, 773, 1017
MvaI CCWGG 6 cut(s) 35, 556, 661, 696, 773, 1017
MvnI CGCG 2 cut(s) 594, 1130
MwoI GCNNNNNNNGC 4 cut(s) 30, 229, 665, 1141
NcoI CCATGG 1 cut(s) 546
NdeII GATC 2 cut(s) 589, 1237
NlaIII CATG 7 cut(s) 392, 442, 447, 550, 611, 796, 1109
NlaIV GGNNCC 1 cut(s) 973
NmuCI GTSAC 3 cut(s) 301, 613, 962
NsbI TGCGCA 1 cut(s) 914
OliI CACNNNNGTG 1 cut(s) 516
PagI TCATGA 2 cut(s) 438, 792
PfeI GAWTC 7 cut(s) 12, 116, 318, 362, 532, 703, 898
PkrI GCNGC 6 cut(s) 23, 165, 222, 342, 381, 585
PleI GAGTC 2 cut(s) 163, 545
PpsI GAGTC 2 cut(s) 163, 545
PshBI ATTAAT 1 cut(s) 1229
Psp124BI GAGCTC 1 cut(s) 511
Psp6I CCWGG 6 cut(s) 33, 554, 659, 694, 771, 1015
PspFI CCCAGC 1 cut(s) 649
PspGI CCWGG 6 cut(s) 33, 554, 659, 694, 771, 1015
PspN4I GGNNCC 1 cut(s) 973
PspPI GGNCC 3 cut(s) 861, 971, 994
PstI CTGCAG 3 cut(s) 168, 384, 588
PstNI CAGNNNCTG 2 cut(s) 163, 721
PvuII CAGCTG 2 cut(s) 324, 653
RsaI GTAC 3 cut(s) 770, 1167, 1197
RsaNI GTAC 3 cut(s) 769, 1166, 1196
RseI CAYNNNNRTG 3 cut(s) 420, 516, 791
SacI GAGCTC 1 cut(s) 511
SaqAI TTAA 3 cut(s) 1089, 1229, 1273
SatI GCNGC 6 cut(s) 22, 164, 221, 341, 380, 584
Sau3AI GATC 2 cut(s) 589, 1237
Sau96I GGNCC 3 cut(s) 861, 971, 994
ScaI AGTACT 1 cut(s) 1197
SchI GAGTC 2 cut(s) 163, 545
ScrFI CCNGG 6 cut(s) 35, 556, 661, 696, 773, 1017
SduI GDGCHC 2 cut(s) 511, 832
SfaNI GCATC 1 cut(s) 832
SfcI CTRYAG 3 cut(s) 164, 380, 584
SinI GGWCC 3 cut(s) 861, 971, 994
SmiMI CAYNNNNRTG 3 cut(s) 420, 516, 791
Sse9I AATT 6 cut(s) 347, 404, 479, 727, 917, 1074
SsiI CCGC 3 cut(s) 22, 581, 594
SspMI CTAG 1 cut(s) 1071
SstI GAGCTC 1 cut(s) 511
StyD4I CCNGG 6 cut(s) 33, 554, 659, 694, 771, 1015
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 2 cut(s) 61, 1033
TaqI TCGA 7 cut(s) 15, 180, 488, 539, 851, 924, 1203
TasI AATT 6 cut(s) 347, 404, 479, 727, 917, 1074
TatI WGTACW 2 cut(s) 1165, 1195
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 7 cut(s) 12, 116, 318, 362, 532, 703, 898
Tru1I TTAA 3 cut(s) 1089, 1229, 1273
Tru9I TTAA 3 cut(s) 1089, 1229, 1273
TscAI CASTG 6 cut(s) 64, 310, 852, 885, 1065, 1247
TseFI GTSAC 3 cut(s) 301, 613, 962
TseI GCWGC 5 cut(s) 163, 220, 340, 379, 583
Tsp45I GTSAC 3 cut(s) 301, 613, 962
TspGWI ACGGA 1 cut(s) 794
TspRI CASTG 6 cut(s) 64, 310, 852, 885, 1065, 1247
VneI GTGCAC 1 cut(s) 828
VpaK11BI GGWCC 3 cut(s) 861, 971, 994
VspI ATTAAT 1 cut(s) 1229
XapI RAATTY 4 cut(s) 347, 404, 479, 917
XbaI TCTAGA 1 cut(s) 1070
XcmI CCANNNNNNNNNTGG 1 cut(s) 657
XspI CTAG 1 cut(s) 1071
ZrmI AGTACT 1 cut(s) 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.