RchiOBHm_Chr3g0463491

Lactosylceramide 4-alpha-galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0013878
Physical Location & Seq
Forward (+)
10832618 .. 10833991
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42980

Sequence Viewer

Length: 1212 bp
ATGATGAGTAGCCGAATCGACAGCCGCCTACTTAGCCTGGCGAAGTTCCACATCTTCTGCACTGTATCACTTGTTGCAATCATCTTCATCATTTATGCAGATGGTATCATATATACTGATTCCCAAAACTCGGTTGAGTTGAAAGATAAAGCTGTTTCTCAGGTGCTGCAGACTCATAGTATCGAACGCCAGTTCATCAAGTCTGATATAACTCGTAAAGCGCTGCGTTCTATGCAAGAAAATGTCCTTGATGTCGTTGAAGTTGATACAGAAGATACAAGTCCTGTTGTTGCTCCTTTCAATGTCACTGAAGAAGAAAGAATCAGCTGGTTTCGGGAAAAGCTGCCCGAATTTGTGATTTTTGATTCAGACGAGAAGGCGCTGCAGTTTCATGGTAGAGTTCTTGAATTCTTTGACCATAAGTGTGATACTCAGTTCTTCATGACATGGATATCGCCTGCGAGGTCTTTTGGAAGCAGAGAATTCCTGTCGATGGAGAGTGTATTCAGAGCTCACCCTCGTGGTTGCTTGATGATTCTTTCGAGAACCATGGACTCCAGGCGAGGTTACAGGCTTCTGAAACCGCTGCAGGATCGCGGGTTCAAAGTTCATGCTGTGACGCCAGACTTGTCATTTCTGTTCAAGAACACCCCAGCTGAGGCCTGGTTTGCTGAGATGAAGACAGGCAACAGAGACCCTGGTGTGTACCTGGACACGGATTTCATTGTCATGAAATCATTTTCGGGGTTGAGGAACTCAATCGGTGCACAAAGTATGGATGCAGTGTCGAAAAACTGGACCAGACTGAACAATGCAGTGCTGGTGTTTGATATGAATCATCCTCTTCTGCGCAAATTCATCGAGGAGTTTGCCTTGACTTTTGATGGGAATAAATGGGGTCACAATGGACCCTATCTTGTTTCCAGAGTGGTCCAAAGAGTGCAAAACAGACCTGGATATAACTTTACAGTCCTGCCTCGTATGGCTTTCTATCCAGTGGATTGGTCTAGAATTGGGGGCTTGTTTAAGAAACCGAAAAACCATGCTGACACTAAATGGATAAACGCGAAGCTGCTTCAGCTTAGTGAGGAGACTTATGGAGTACACCTATGGAACAAGCAAAGTAGCAGAGTACTAATCGAAGAAGGAAGCATTATGCAGAGATTAATCTCCGATCACTGCGTCATCTGTAAAGACATATATAGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

403

Amino Acids

46.57

Weight (kDa)

8.67

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gb3_synth PF04572 276 - 398 8.4e-30 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38152
fragaria_vesca FvH4_6g11720
malus_domestica MD04G1149800.v1.1 MD12G1164200.v1.1
prunus_persica Prupe.6G274700_v2.0.a1
pyrus_communis pycom04g13530
rosa_chinensis RchiOBHm_Chr3g0463491
rosa_laevigata RLG00000019555 RLG00000024783
rosa_multiflora Rmu_sc0000923.1_g000020
rosa_roxburghii Rroxscaffold_6G00416520
rosa_rugosa Rorug03G0065100
rosa_samantha Rh3AG122300 Rh3BG127000 Rh3CG128900 Rh3DG127800
rosa_wichuraiana Rw0G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 851
AccII CGCG 2 cut(s) 597, 1067
AciI CCGC 3 cut(s) 25, 584, 597
AclWI GGATC 1 cut(s) 600
AcsI RAATTY 4 cut(s) 350, 407, 482, 854
AcuI CTGAAG 2 cut(s) 330, 1061
AcyI GRCGYC 1 cut(s) 620
AfaI GTAC 3 cut(s) 707, 1104, 1134
AfeI AGCGCT 1 cut(s) 222
AfiI CCNNNNNNNGG 4 cut(s) 130, 493, 658, 715
AgsI TTSAA 6 cut(s) 142, 260, 301, 407, 604, 643
AjnI CCWGG 6 cut(s) 36, 557, 662, 697, 708, 952
AleI CACNNNNGTG 1 cut(s) 519
AloI GAACNNNNNNTCC 2 cut(s) 584, 616
AluBI AGCT 7 cut(s) 152, 327, 343, 512, 656, 1072, 1081
AluI AGCT 7 cut(s) 152, 327, 343, 512, 656, 1072, 1081
Alw21I GWGCWC 2 cut(s) 514, 769
Alw26I GTCTC 2 cut(s) 687, 1085
Alw44I GTGCAC 1 cut(s) 765
AlwI GGATC 1 cut(s) 600
AlwNI CAGNNNCTG 1 cut(s) 166
Aor51HI AGCGCT 1 cut(s) 222
AoxI GGCC 1 cut(s) 660
ApaLI GTGCAC 1 cut(s) 765
ApeKI GCWGC 6 cut(s) 166, 223, 343, 382, 586, 1072
ApoI RAATTY 4 cut(s) 350, 407, 482, 854
AseI ATTAAT 1 cut(s) 1166
AspLEI GCGC 3 cut(s) 223, 382, 852
AspS9I GGNCC 3 cut(s) 798, 908, 931
AsuHPI GGTGA 1 cut(s) 506
AvaII GGWCC 3 cut(s) 798, 908, 931
BaeGI GKGCMC 1 cut(s) 769
BanII GRGCYC 1 cut(s) 514
BauI CACGAG 1 cut(s) 519
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 2 cut(s) 514, 769
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 6 cut(s) 153, 210, 330, 369, 573, 1059
BccI CCATC 3 cut(s) 95, 487, 878
BcgI CGANNNNNNTGC 4 cut(s) 841, 851, 875, 885
BciT130I CCWGG 6 cut(s) 38, 559, 664, 699, 710, 954
BcoDI GTCTC 2 cut(s) 687, 1085
BfaI CTAG 1 cut(s) 1008
BfmI CTRYAG 3 cut(s) 167, 383, 587
BfoI RGCGCY 2 cut(s) 224, 383
BisI GCNGC 7 cut(s) 25, 167, 224, 344, 383, 587, 1073
BlsI GCNGC 7 cut(s) 26, 168, 225, 345, 384, 588, 1074
BmcAI AGTACT 1 cut(s) 1134
Bme1390I CCNGG 6 cut(s) 38, 559, 664, 699, 710, 954
Bme18I GGWCC 3 cut(s) 798, 908, 931
BmgT120I GGNCC 3 cut(s) 798, 908, 931
BmiI GGNNCC 1 cut(s) 910
BmrFI CCNGG 6 cut(s) 38, 559, 664, 699, 710, 954
BmsI GCATC 1 cut(s) 769
BpiI GAAGAC 1 cut(s) 686
BpmI CTGGAG 1 cut(s) 541
Bpu10I CCTNAGC 1 cut(s) 657
BsaBI GATNNNNATC 1 cut(s) 834
BsaHI GRCGYC 1 cut(s) 620
BsaI GGTCTC 1 cut(s) 687
BsaJI CCNNGG 2 cut(s) 549, 697
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 493, 658, 715
Bse1I ACTGG 3 cut(s) 190, 800, 995
Bse8I GATNNNNATC 1 cut(s) 834
BseBI CCWGG 6 cut(s) 38, 559, 664, 699, 710, 954
BseDI CCNNGG 2 cut(s) 549, 697
BseGI GGATG 2 cut(s) 784, 838
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 4 cut(s) 130, 493, 658, 715
BseMII CTCAG 4 cut(s) 173, 446, 648, 663
BseNI ACTGG 3 cut(s) 190, 800, 995
BseRI GAGGAG 2 cut(s) 878, 1103
BseSI GKGCMC 1 cut(s) 769
BseXI GCAGC 6 cut(s) 153, 210, 330, 369, 573, 1059
BseYI CCCAGC 1 cut(s) 652
BsgI GTGCAG 1 cut(s) 43
Bsh1236I CGCG 2 cut(s) 597, 1067
BshFI GGCC 1 cut(s) 662
BsiHKAI GWGCWC 2 cut(s) 514, 769
BslI CCNNNNNNNGG 4 cut(s) 130, 493, 658, 715
BsmAI GTCTC 2 cut(s) 687, 1085
BsnI GGCC 1 cut(s) 662
Bso31I GGTCTC 1 cut(s) 687
Bsp1286I GDGCHC 2 cut(s) 514, 769
Bsp143I GATC 2 cut(s) 592, 1174
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 3 cut(s) 25, 584, 597
BspANI GGCC 1 cut(s) 662
BspCNI CTCAG 4 cut(s) 172, 445, 649, 664
BspFNI CGCG 2 cut(s) 597, 1067
BspHI TCATGA 2 cut(s) 441, 729
BspLI GGNNCC 1 cut(s) 910
BspMAI CTGCAG 3 cut(s) 171, 387, 591
BspPI GGATC 1 cut(s) 600
BspTNI GGTCTC 1 cut(s) 687
BsrI ACTGG 3 cut(s) 190, 800, 995
BssECI CCNNGG 2 cut(s) 549, 697
BssMI GATC 2 cut(s) 592, 1174
BssNI GRCGYC 1 cut(s) 620
BssSI CACGAG 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 549
Bst2BI CACGAG 1 cut(s) 519
Bst2UI CCWGG 6 cut(s) 38, 559, 664, 699, 710, 954
Bst4CI ACNGT 2 cut(s) 64, 970
Bst6I CTCTTC 1 cut(s) 849
BstACI GRCGYC 1 cut(s) 620
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 6 cut(s) 32, 159, 432, 657, 672, 1082
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 784, 838
BstFNI CGCG 2 cut(s) 597, 1067
BstH2I RGCGCY 2 cut(s) 224, 383
BstHHI GCGC 3 cut(s) 223, 382, 852
BstKTI GATC 2 cut(s) 595, 1177
BstMAI GTCTC 2 cut(s) 687, 1085
BstMBI GATC 2 cut(s) 592, 1174
BstMWI GCNNNNNNNGC 4 cut(s) 33, 232, 668, 1078
BstNI CCWGG 6 cut(s) 38, 559, 664, 699, 710, 954
BstSCI CCNGG 6 cut(s) 36, 557, 662, 697, 708, 952
BstSFI CTRYAG 3 cut(s) 167, 383, 587
BstSLI GKGCMC 1 cut(s) 769
BstUI CGCG 2 cut(s) 597, 1067
BstV1I GCAGC 6 cut(s) 153, 210, 330, 369, 573, 1059
BstV2I GAAGAC 1 cut(s) 686
BstXI CCANNNNNNTGG 1 cut(s) 1002
BsuRI GGCC 1 cut(s) 662
BtgI CCRYGG 1 cut(s) 549
BtsCI GGATG 2 cut(s) 784, 838
BtsI GCAGTG 3 cut(s) 789, 822, 1177
BtsIMutI CAGTG 6 cut(s) 60, 306, 789, 822, 1002, 1177
Cac8I GCNNGC 1 cut(s) 459
CaiI CAGNNNCTG 1 cut(s) 166
CciI TCATGA 2 cut(s) 441, 729
CfoI GCGC 3 cut(s) 223, 382, 852
Cfr13I GGNCC 3 cut(s) 798, 908, 931
CseI GACGC 2 cut(s) 628, 1171
Csp6I GTAC 3 cut(s) 706, 1103, 1133
CviAII CATG 7 cut(s) 392, 442, 447, 550, 611, 730, 1043
CviQI GTAC 3 cut(s) 706, 1103, 1133
DdeI CTNAG 6 cut(s) 32, 159, 432, 657, 672, 1082
DpnI GATC 2 cut(s) 594, 1176
DpnII GATC 2 cut(s) 592, 1174
Eam1104I CTCTTC 1 cut(s) 849
EarI CTCTTC 1 cut(s) 849
Ecl136II GAGCTC 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 662
Eco24I GRGCYC 1 cut(s) 514
Eco31I GGTCTC 1 cut(s) 687
Eco32I GATATC 1 cut(s) 453
Eco47I GGWCC 3 cut(s) 798, 908, 931
Eco47III AGCGCT 1 cut(s) 222
Eco53kI GAGCTC 1 cut(s) 512
Eco57I CTGAAG 2 cut(s) 330, 1061
EcoICRI GAGCTC 1 cut(s) 512
EcoRI GAATTC 2 cut(s) 407, 482
EcoRII CCWGG 6 cut(s) 36, 557, 662, 697, 708, 952
EcoRV GATATC 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 549
EcoT38I GRGCYC 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 7 cut(s) 395, 445, 450, 553, 614, 733, 1046
FatI CATG 7 cut(s) 391, 441, 446, 549, 610, 729, 1042
FauI CCCGC 1 cut(s) 590
Fnu4HI GCNGC 7 cut(s) 25, 167, 224, 344, 383, 587, 1073
FokI GGATG 2 cut(s) 791, 825
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 7 cut(s) 25, 167, 224, 344, 383, 587, 1073
FspBI CTAG 1 cut(s) 1008
FspI TGCGCA 1 cut(s) 851
GlaI GCGC 3 cut(s) 222, 381, 851
GluI GCNGC 7 cut(s) 25, 167, 224, 344, 383, 587, 1073
GsaI CCCAGC 1 cut(s) 656
GsuI CTGGAG 1 cut(s) 541
HaeII RGCGCY 2 cut(s) 224, 383
HaeIII GGCC 1 cut(s) 662
HgaI GACGC 2 cut(s) 628, 1171
HhaI GCGC 3 cut(s) 223, 382, 852
Hin1I GRCGYC 1 cut(s) 620
Hin1II CATG 7 cut(s) 395, 445, 450, 553, 614, 733, 1046
Hin6I GCGC 3 cut(s) 221, 380, 850
HinP1I GCGC 3 cut(s) 221, 380, 850
HinfI GANTC 8 cut(s) 15, 119, 172, 321, 365, 535, 554, 835
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 3 cut(s) 706, 767, 1105
Hpy188I TCNGA 5 cut(s) 205, 370, 509, 579, 1174
Hpy188III TCNNGA 8 cut(s) 335, 404, 442, 543, 643, 730, 924, 1008
Hpy8I GTNNAC 3 cut(s) 706, 767, 1105
HpyAV CCTTC 2 cut(s) 370, 1139
HpyCH4III ACNGT 2 cut(s) 64, 970
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 232, 668, 1078
HpyF3I CTNAG 6 cut(s) 32, 159, 432, 657, 672, 1082
Hsp92I GRCGYC 1 cut(s) 620
Hsp92II CATG 7 cut(s) 395, 445, 450, 553, 614, 733, 1046
HspAI GCGC 3 cut(s) 221, 380, 850
Kzo9I GATC 2 cut(s) 592, 1174
LmnI GCTCC 1 cut(s) 298
Lsp1109I GCAGC 6 cut(s) 153, 210, 330, 369, 573, 1059
LweI GCATC 1 cut(s) 769
MaeI CTAG 1 cut(s) 1008
MaeIII GTNAC 4 cut(s) 304, 566, 616, 899
MalI GATC 2 cut(s) 594, 1176
MboI GATC 2 cut(s) 592, 1174
MboII GAAGA 9 cut(s) 46, 76, 284, 323, 326, 430, 691, 836, 1154
MhlI GDGCHC 2 cut(s) 514, 769
MluCI AATT 5 cut(s) 350, 407, 482, 854, 1011
MlyI GAGTC 2 cut(s) 166, 548
MnlI CCTC 9 cut(s) 456, 528, 557, 652, 744, 852, 856, 987, 1081
MseI TTAA 3 cut(s) 1026, 1166, 1210
MslI CAYNNNNRTG 3 cut(s) 423, 519, 728
MspA1I CMGCKG 3 cut(s) 327, 586, 656
MspR9I CCNGG 6 cut(s) 38, 559, 664, 699, 710, 954
MvaI CCWGG 6 cut(s) 38, 559, 664, 699, 710, 954
MvnI CGCG 2 cut(s) 597, 1067
MwoI GCNNNNNNNGC 4 cut(s) 33, 232, 668, 1078
NcoI CCATGG 1 cut(s) 549
NdeII GATC 2 cut(s) 592, 1174
NlaIII CATG 7 cut(s) 395, 445, 450, 553, 614, 733, 1046
NlaIV GGNNCC 1 cut(s) 910
NmuCI GTSAC 3 cut(s) 304, 616, 899
NsbI TGCGCA 1 cut(s) 851
OliI CACNNNNGTG 1 cut(s) 519
PagI TCATGA 2 cut(s) 441, 729
PceI AGGCCT 1 cut(s) 662
PfeI GAWTC 6 cut(s) 15, 119, 321, 365, 535, 835
PkrI GCNGC 7 cut(s) 26, 168, 225, 345, 384, 588, 1074
PleI GAGTC 2 cut(s) 166, 548
PpsI GAGTC 2 cut(s) 166, 548
PshBI ATTAAT 1 cut(s) 1166
Psp124BI GAGCTC 1 cut(s) 514
Psp6I CCWGG 6 cut(s) 36, 557, 662, 697, 708, 952
PspFI CCCAGC 1 cut(s) 652
PspGI CCWGG 6 cut(s) 36, 557, 662, 697, 708, 952
PspN4I GGNNCC 1 cut(s) 910
PspPI GGNCC 3 cut(s) 798, 908, 931
PstI CTGCAG 3 cut(s) 171, 387, 591
PstNI CAGNNNCTG 1 cut(s) 166
PvuII CAGCTG 2 cut(s) 327, 656
RsaI GTAC 3 cut(s) 707, 1104, 1134
RsaNI GTAC 3 cut(s) 706, 1103, 1133
RseI CAYNNNNRTG 3 cut(s) 423, 519, 728
SacI GAGCTC 1 cut(s) 514
SaqAI TTAA 3 cut(s) 1026, 1166, 1210
SatI GCNGC 7 cut(s) 25, 167, 224, 344, 383, 587, 1073
Sau3AI GATC 2 cut(s) 592, 1174
Sau96I GGNCC 3 cut(s) 798, 908, 931
ScaI AGTACT 1 cut(s) 1134
SchI GAGTC 2 cut(s) 166, 548
ScrFI CCNGG 6 cut(s) 38, 559, 664, 699, 710, 954
SduI GDGCHC 2 cut(s) 514, 769
SfaNI GCATC 1 cut(s) 769
SfcI CTRYAG 3 cut(s) 167, 383, 587
SinI GGWCC 3 cut(s) 798, 908, 931
SmiMI CAYNNNNRTG 3 cut(s) 423, 519, 728
Sse9I AATT 5 cut(s) 350, 407, 482, 854, 1011
SseBI AGGCCT 1 cut(s) 662
SsiI CCGC 3 cut(s) 25, 584, 597
SspMI CTAG 1 cut(s) 1008
SstI GAGCTC 1 cut(s) 514
StuI AGGCCT 1 cut(s) 662
StyD4I CCNGG 6 cut(s) 36, 557, 662, 697, 708, 952
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 2 cut(s) 64, 970
TaqI TCGA 7 cut(s) 18, 183, 491, 542, 788, 861, 1140
TasI AATT 5 cut(s) 350, 407, 482, 854, 1011
TatI WGTACW 2 cut(s) 1102, 1132
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 6 cut(s) 15, 119, 321, 365, 535, 835
Tru1I TTAA 3 cut(s) 1026, 1166, 1210
Tru9I TTAA 3 cut(s) 1026, 1166, 1210
TscAI CASTG 6 cut(s) 67, 313, 789, 822, 1002, 1184
TseFI GTSAC 3 cut(s) 304, 616, 899
TseI GCWGC 6 cut(s) 166, 223, 343, 382, 586, 1072
Tsp45I GTSAC 3 cut(s) 304, 616, 899
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 6 cut(s) 67, 313, 789, 822, 1002, 1184
VneI GTGCAC 1 cut(s) 765
VpaK11BI GGWCC 3 cut(s) 798, 908, 931
VspI ATTAAT 1 cut(s) 1166
XapI RAATTY 4 cut(s) 350, 407, 482, 854
XbaI TCTAGA 1 cut(s) 1007
XcmI CCANNNNNNNNNTGG 1 cut(s) 660
XspI CTAG 1 cut(s) 1008
ZrmI AGTACT 1 cut(s) 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.