pycom04g13530

Lactosylceramide 4-alpha-galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013878
Physical Location & Seq
Reverse (-)
16571818 .. 16573111
1294 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g13530.1

Sequence Viewer

Length: 1239 bp
ATGACCATGACTCGAATCGACAGCCGCCTTCTTAGCCTTGCCAAATTTCGCATCTTCTATACTCTTTTAATTGTTGCAATCATCGTCCTCGTCTATGCCGCTAATATCATCCATAGCGACTCGGAACAGTCAGTAGCGTCGAACAACAAGGCAATTTCTCCGTTGCTGCAGATTCTGCGTTCCATGCAGGAGGAGACCATTGAAGTTGGTAGAGAAAACACAAGGCCTCTTGTTCCTCCTTTCAATGGCACAGAAGAAGGGAGAATTGCTTGGTTCCGAAGCATGCTGTCGGAATTTCACATTTTCAAGTCAGACAAGTCTGCAAAGCAATTCCATGGCCGTGTTCTGGAATTCTTTAACAAGGAGTGTGAGGGTCAGTTCTTTATGACATGGATTTCGCCAGCGAGGTCTTTTGGGAGCAGAGAGTTCTTGACAATGGAGAGTCTCTTCAAAGCCCACCCTCGTGGATGTTTGATGATTCTATCGAGAACTATGGACTCCAAGTGCGGTTACAGAATTCTGAAACCGCTGAAGGATAGCGGGTTCAAGGTTCATGCTGTGACACCTGACTTGTCATTTCTGTTGAAAAACACGCCGGCAGAATCCTGGTTTGCGGAGATGAAGAGAGGCAAGAGAGACCCTGGTGAGATTCCATTAGCCCAGAACCTGTCTAATTTGATGAGACTCGCTGTTCTATATAAGTATGGAGGTGTTTACTTAGACACAGACTTCATCGTCTTGAAATCGTTCTCGGGATTGAGAAACTCGATCGGTGCACAAAGTGTGGATGTGGTTTCGAAGAACTGGACCAGATTAAACAACGCAGTTCTGATCTTTGATATGAATCATCCCCTTCTGTACAAATTCATGGAGGAATTTGCCCTCACTTTTGATGGGAATAAATGGGGGCATAATGGTCCCTACCTGGTTTCCAGAGTGGTCCTGAGGGTGCAAAACCGACCCGGTTATAACTTCACGATTCTACCCCCCCTCGCCTTTTACCCCGTCGATTGGAGTAGGATCGGAGGATTGTTTAAGAAGCCGGGAAGTCCGGCTCATTCTCGATGGATAAGAGAAAAGCTGCTTCAGCTGAGCGGGGAGACTTACGGAGTACACCTATGGAACAAGCAAAGCAGCAGAGAAGTAGTTGAAGAAGGAAGCCTTATGCAGAGATTAATCTCCGATCGCTGTATCATTTGCAACGACATATACAGTTCTTCAAGAATTTTAATCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00601 ko00603 ko01100 map00601 map00603 map01100
Pfam Domains
Protein Families
Gb3_synth Gly_transf_sug

Protein Analysis

413

Amino Acids

47.2

Weight (kDa)

9.48

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly_transf_sug PF04488 141 - 261 4.4e-23 Glycosyltransferase sugar-binding region containing DXD motif
Gb3_synth PF04572 279 - 402 5.5e-27 Alpha 1,4-glycosyltransferase conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013878)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38152
fragaria_vesca FvH4_6g11720
malus_domestica MD04G1149800.v1.1 MD12G1164200.v1.1
prunus_persica Prupe.6G274700_v2.0.a1
pyrus_communis pycom04g13530
rosa_chinensis RchiOBHm_Chr3g0463491
rosa_laevigata RLG00000019555 RLG00000024783
rosa_multiflora Rmu_sc0000923.1_g000020
rosa_roxburghii Rroxscaffold_6G00416520
rosa_rugosa Rorug03G0065100
rosa_samantha Rh3AG122300 Rh3BG127000 Rh3CG128900 Rh3DG127800
rosa_wichuraiana Rw0G010600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 969
AasI GACNNNNNNGTC 1 cut(s) 734
AccBSI CCGCTC 1 cut(s) 1095
AciI CCGC 7 cut(s) 25, 99, 507, 527, 540, 614, 1095
AclWI GGATC 1 cut(s) 1028
AcoI YGGCCR 1 cut(s) 337
AcsI RAATTY 7 cut(s) 44, 293, 350, 516, 863, 875, 1224
AcuI CTGAAG 2 cut(s) 551, 1070
AdeI CACNNNGTG 1 cut(s) 782
AfaI GTAC 2 cut(s) 860, 1113
AfiI CCNNNNNNNGG 3 cut(s) 245, 346, 1011
AgsI TTSAA 9 cut(s) 203, 244, 307, 451, 547, 586, 742, 1151, 1221
AjnI CCWGG 3 cut(s) 605, 640, 924
AleI CACNNNNGTG 1 cut(s) 462
AloI GAACNNNNNNTCC 2 cut(s) 527, 559
AluBI AGCT 2 cut(s) 1081, 1090
AluI AGCT 2 cut(s) 1081, 1090
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 5 cut(s) 188, 449, 630, 676, 1094
Alw44I GTGCAC 1 cut(s) 774
AlwI GGATC 1 cut(s) 1028
AlwNI CAGNNNCTG 2 cut(s) 175, 667
Ama87I CYCGRG 1 cut(s) 751
AoxI GGCC 2 cut(s) 224, 337
ApaLI GTGCAC 1 cut(s) 774
ApeKI GCWGC 3 cut(s) 166, 1081, 1134
ApoI RAATTY 7 cut(s) 44, 293, 350, 516, 863, 875, 1224
ArsI GACNNNNNNTTYG 2 cut(s) 379, 411
AseI ATTAAT 1 cut(s) 1175
Asp700I GAANNNNTTC 1 cut(s) 746
AspS9I GGNCC 3 cut(s) 807, 917, 940
AsuC2I CCSGG 2 cut(s) 963, 1044
AsuHPI GGTGA 1 cut(s) 656
AsuII TTCGAA 1 cut(s) 797
AvaI CYCGRG 1 cut(s) 751
AvaII GGWCC 3 cut(s) 807, 917, 940
AxyI CCTNAGG 1 cut(s) 944
BaeGI GKGCMC 1 cut(s) 778
BauI CACGAG 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 778
BbvI GCAGC 3 cut(s) 153, 1068, 1146
BccI CCATC 2 cut(s) 887, 1059
BceAI ACGGC 1 cut(s) 324
BciT130I CCWGG 3 cut(s) 607, 642, 926
BcnI CCSGG 2 cut(s) 963, 1044
BcoDI GTCTC 5 cut(s) 188, 449, 630, 676, 1094
BfmI CTRYAG 1 cut(s) 167
BisI GCNGC 5 cut(s) 25, 99, 167, 1082, 1135
BlpI GCTNAGC 1 cut(s) 1091
BlsI GCNGC 5 cut(s) 26, 100, 168, 1083, 1136
Bme1390I CCNGG 5 cut(s) 607, 642, 926, 963, 1044
Bme18I GGWCC 3 cut(s) 807, 917, 940
BmeT110I CYCGRG 1 cut(s) 751
BmgT120I GGNCC 3 cut(s) 807, 917, 940
BmiI GGNNCC 2 cut(s) 275, 919
BmrFI CCNGG 5 cut(s) 607, 642, 926, 963, 1044
BmsI GCATC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 1091
Bpu14I TTCGAA 1 cut(s) 797
BpuMI CCSGG 2 cut(s) 963, 1044
BsaBI GATNNNNATC 1 cut(s) 843
BsaI GGTCTC 2 cut(s) 188, 630
BsaJI CCNNGG 2 cut(s) 334, 640
Bsc4I CCNNNNNNNGG 3 cut(s) 245, 346, 1011
Bse118I RCCGGY 1 cut(s) 595
Bse1I ACTGG 1 cut(s) 809
Bse21I CCTNAGG 1 cut(s) 944
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 3 cut(s) 607, 642, 926
BseDI CCNNGG 2 cut(s) 334, 640
BseGI GGATG 4 cut(s) 108, 473, 793, 847
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 3 cut(s) 245, 346, 1011
BseMII CTCAG 2 cut(s) 935, 1082
BseNI ACTGG 1 cut(s) 809
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 778
BseXI GCAGC 3 cut(s) 153, 1068, 1146
Bsh1285I CGRYCG 2 cut(s) 771, 1186
BshFI GGCC 2 cut(s) 226, 339
BsiEI CGRYCG 2 cut(s) 771, 1186
BsiHKAI GWGCWC 1 cut(s) 778
BsiHKCI CYCGRG 1 cut(s) 751
BsiSI CCGG 4 cut(s) 596, 963, 1043, 1052
BslFI GGGAC 1 cut(s) 903
BslI CCNNNNNNNGG 3 cut(s) 245, 346, 1011
BsmAI GTCTC 5 cut(s) 188, 449, 630, 676, 1094
BsmFI GGGAC 1 cut(s) 903
BsnI GGCC 2 cut(s) 226, 339
Bso31I GGTCTC 2 cut(s) 188, 630
BsoBI CYCGRG 1 cut(s) 751
Bsp119I TTCGAA 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 778
Bsp1407I TGTACA 1 cut(s) 858
Bsp143I GATC 4 cut(s) 768, 831, 1020, 1183
Bsp1720I GCTNAGC 1 cut(s) 1091
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 7 cut(s) 25, 99, 507, 527, 540, 614, 1095
BspANI GGCC 2 cut(s) 226, 339
BspCNI CTCAG 2 cut(s) 936, 1083
BspLI GGNNCC 2 cut(s) 275, 919
BspMAI CTGCAG 1 cut(s) 171
BspPI GGATC 1 cut(s) 1028
BspT104I TTCGAA 1 cut(s) 797
BspTNI GGTCTC 2 cut(s) 188, 630
BsrBI CCGCTC 1 cut(s) 1095
BsrFI RCCGGY 1 cut(s) 595
BsrGI TGTACA 1 cut(s) 858
BsrI ACTGG 1 cut(s) 809
BssAI RCCGGY 1 cut(s) 595
BssECI CCNNGG 2 cut(s) 334, 640
BssMI GATC 4 cut(s) 768, 831, 1020, 1183
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 3 cut(s) 607, 642, 926
Bst4CI ACNGT 2 cut(s) 129, 1214
Bst6I CTCTTC 2 cut(s) 452, 617
BstAPI GCANNNNNTGC 1 cut(s) 175
BstAUI TGTACA 1 cut(s) 858
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 3 cut(s) 284, 402, 597
BstDEI CTNAG 4 cut(s) 32, 718, 944, 1091
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 4 cut(s) 108, 473, 793, 847
BstKTI GATC 4 cut(s) 771, 834, 1023, 1186
BstMAI GTCTC 5 cut(s) 188, 449, 630, 676, 1094
BstMBI GATC 4 cut(s) 768, 831, 1020, 1183
BstMCI CGRYCG 2 cut(s) 771, 1186
BstMWI GCNNNNNNNGC 4 cut(s) 33, 175, 184, 1087
BstNI CCWGG 3 cut(s) 607, 642, 926
BstNSI RCATGY 1 cut(s) 286
BstSCI CCNGG 5 cut(s) 605, 640, 924, 961, 1042
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 778
BstV1I GCAGC 3 cut(s) 153, 1068, 1146
BstXI CCANNNNNNTGG 1 cut(s) 464
Bsu36I CCTNAGG 1 cut(s) 944
BsuRI GGCC 2 cut(s) 226, 339
BtgI CCRYGG 1 cut(s) 334
BtsCI GGATG 4 cut(s) 108, 473, 793, 847
Cac8I GCNNGC 3 cut(s) 284, 402, 597
CaiI CAGNNNCTG 2 cut(s) 175, 667
Cfr10I RCCGGY 1 cut(s) 595
Cfr13I GGNCC 3 cut(s) 807, 917, 940
CseI GACGC 1 cut(s) 126
CsiI ACCWGGT 1 cut(s) 924
Csp6I GTAC 2 cut(s) 859, 1112
CviAII CATG 7 cut(s) 7, 184, 283, 335, 390, 554, 868
CviQI GTAC 2 cut(s) 859, 1112
DdeI CTNAG 4 cut(s) 32, 718, 944, 1091
DpnI GATC 4 cut(s) 770, 833, 1022, 1185
DpnII GATC 4 cut(s) 768, 831, 1020, 1183
DraIII CACNNNGTG 1 cut(s) 782
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 2 cut(s) 452, 617
EarI CTCTTC 2 cut(s) 452, 617
Eco130I CCWWGG 1 cut(s) 334
Eco147I AGGCCT 1 cut(s) 226
Eco31I GGTCTC 2 cut(s) 188, 630
Eco47I GGWCC 3 cut(s) 807, 917, 940
Eco57I CTGAAG 2 cut(s) 551, 1070
Eco81I CCTNAGG 1 cut(s) 944
Eco88I CYCGRG 1 cut(s) 751
EcoRI GAATTC 2 cut(s) 350, 516
EcoRII CCWGG 3 cut(s) 605, 640, 924
EcoT14I CCWWGG 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 7 cut(s) 10, 187, 286, 338, 393, 557, 871
FalI AAGNNNNNCTT 2 cut(s) 1146, 1178
FaqI GGGAC 1 cut(s) 903
FatI CATG 7 cut(s) 6, 183, 282, 334, 389, 553, 867
FauI CCCGC 2 cut(s) 533, 1088
Fnu4HI GCNGC 5 cut(s) 25, 99, 167, 1082, 1135
FokI GGATG 4 cut(s) 95, 480, 800, 834
Fsp4HI GCNGC 5 cut(s) 25, 99, 167, 1082, 1135
GluI GCNGC 5 cut(s) 25, 99, 167, 1082, 1135
HaeIII GGCC 2 cut(s) 226, 339
HapII CCGG 4 cut(s) 596, 963, 1043, 1052
HgaI GACGC 1 cut(s) 126
Hin1II CATG 7 cut(s) 10, 187, 286, 338, 393, 557, 871
HpaII CCGG 4 cut(s) 596, 963, 1043, 1052
HphI GGTGA 1 cut(s) 656
Hpy166II GTNNAC 3 cut(s) 715, 776, 1114
Hpy188I TCNGA 8 cut(s) 124, 278, 292, 313, 522, 831, 1025, 1183
Hpy8I GTNNAC 3 cut(s) 715, 776, 1114
Hpy99I CGWCG 2 cut(s) 142, 1010
HpyAV CCTTC 5 cut(s) 38, 251, 526, 863, 1148
HpyCH4III ACNGT 2 cut(s) 129, 1214
HpyCH4V TGCA 8 cut(s) 77, 169, 187, 323, 776, 952, 1168, 1200
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 175, 184, 1087
HpyF3I CTNAG 4 cut(s) 32, 718, 944, 1091
Hsp92II CATG 7 cut(s) 10, 187, 286, 338, 393, 557, 871
KroI GCCGGC 1 cut(s) 595
KroNI GCCGGC 1 cut(s) 597
Kzo9I GATC 4 cut(s) 768, 831, 1020, 1183
LmnI GCTCC 1 cut(s) 417
Lsp1109I GCAGC 3 cut(s) 153, 1068, 1146
LweI GCATC 1 cut(s) 60
MabI ACCWGGT 1 cut(s) 924
MaeIII GTNAC 2 cut(s) 509, 559
MalI GATC 4 cut(s) 770, 833, 1022, 1185
MbiI CCGCTC 1 cut(s) 1095
MboI GATC 4 cut(s) 768, 831, 1020, 1183
MboII GAAGA 7 cut(s) 46, 266, 439, 634, 811, 1163, 1209
MhlI GDGCHC 1 cut(s) 778
MlyI GAGTC 5 cut(s) 4, 113, 451, 491, 678
MmeI TCCRAC 1 cut(s) 270
MroNI GCCGGC 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 746
MseI TTAA 6 cut(s) 68, 357, 815, 1035, 1175, 1229
MslI CAYNNNNRTG 2 cut(s) 339, 462
MspA1I CMGCKG 2 cut(s) 529, 1090
MspI CCGG 4 cut(s) 596, 963, 1043, 1052
MspR9I CCNGG 5 cut(s) 607, 642, 926, 963, 1044
MvaI CCWGG 3 cut(s) 607, 642, 926
MwoI GCNNNNNNNGC 4 cut(s) 33, 175, 184, 1087
NaeI GCCGGC 1 cut(s) 597
NciI CCSGG 2 cut(s) 963, 1044
NcoI CCATGG 1 cut(s) 334
NdeII GATC 4 cut(s) 768, 831, 1020, 1183
NgoMIV GCCGGC 1 cut(s) 595
NlaIII CATG 7 cut(s) 10, 187, 286, 338, 393, 557, 871
NlaIV GGNNCC 2 cut(s) 275, 919
NmuCI GTSAC 1 cut(s) 559
NspI RCATGY 1 cut(s) 286
NspV TTCGAA 1 cut(s) 797
OliI CACNNNNGTG 1 cut(s) 462
PaeI GCATGC 1 cut(s) 286
PceI AGGCCT 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 597
PdmI GAANNNNTTC 1 cut(s) 746
PfeI GAWTC 7 cut(s) 15, 172, 478, 602, 649, 844, 979
PkrI GCNGC 5 cut(s) 26, 100, 168, 1083, 1136
Ple19I CGATCG 2 cut(s) 771, 1186
PleI GAGTC 5 cut(s) 4, 113, 450, 491, 678
PpsI GAGTC 5 cut(s) 4, 113, 450, 491, 678
PshBI ATTAAT 1 cut(s) 1175
PsiI TTATAA 1 cut(s) 969
Psp6I CCWGG 3 cut(s) 605, 640, 924
PspGI CCWGG 3 cut(s) 605, 640, 924
PspN4I GGNNCC 2 cut(s) 275, 919
PspPI GGNCC 3 cut(s) 807, 917, 940
PstI CTGCAG 1 cut(s) 171
PstNI CAGNNNCTG 2 cut(s) 175, 667
PvuI CGATCG 2 cut(s) 771, 1186
PvuII CAGCTG 1 cut(s) 1090
RsaI GTAC 2 cut(s) 860, 1113
RsaNI GTAC 2 cut(s) 859, 1112
RseI CAYNNNNRTG 2 cut(s) 339, 462
SaqAI TTAA 6 cut(s) 68, 357, 815, 1035, 1175, 1229
SatI GCNGC 5 cut(s) 25, 99, 167, 1082, 1135
Sau3AI GATC 4 cut(s) 768, 831, 1020, 1183
Sau96I GGNCC 3 cut(s) 807, 917, 940
SchI GAGTC 5 cut(s) 4, 113, 451, 491, 678
ScrFI CCNGG 5 cut(s) 607, 642, 926, 963, 1044
SduI GDGCHC 1 cut(s) 778
SetI ASST 9 cut(s) 410, 552, 568, 669, 712, 927, 1083, 1092, 1119
SexAI ACCWGGT 1 cut(s) 924
SfaNI GCATC 1 cut(s) 60
SfcI CTRYAG 1 cut(s) 167
SfuI TTCGAA 1 cut(s) 797
SinI GGWCC 3 cut(s) 807, 917, 940
SmiMI CAYNNNNRTG 2 cut(s) 339, 462
SphI GCATGC 1 cut(s) 286
SseBI AGGCCT 1 cut(s) 226
SsiI CCGC 7 cut(s) 25, 99, 507, 527, 540, 614, 1095
StuI AGGCCT 1 cut(s) 226
StyD4I CCNGG 5 cut(s) 605, 640, 924, 961, 1042
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 2 cut(s) 129, 1214
TaqI TCGA 9 cut(s) 13, 18, 140, 485, 767, 797, 1008, 1063, 1233
TatI WGTACW 2 cut(s) 858, 1111
TauI GCSGC 2 cut(s) 27, 101
TfiI GAWTC 7 cut(s) 15, 172, 478, 602, 649, 844, 979
Tru1I TTAA 6 cut(s) 68, 357, 815, 1035, 1175, 1229
Tru9I TTAA 6 cut(s) 68, 357, 815, 1035, 1175, 1229
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 3 cut(s) 166, 1081, 1134
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 5 cut(s) 542, 635, 721, 856, 857
TspGWI ACGGA 2 cut(s) 150, 1122
VneI GTGCAC 1 cut(s) 774
VpaK11BI GGWCC 3 cut(s) 807, 917, 940
VspI ATTAAT 1 cut(s) 1175
XapI RAATTY 7 cut(s) 44, 293, 350, 516, 863, 875, 1224
XceI RCATGY 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.