Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009175

Physical Location & Seq

Genomic Coordinates Forward (+)

25572114 .. 25575362

Gene Structure 3249 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1165100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGCAGCAGTCGCAGAGGTGGAAGTCGAGCAAGAAGAAAACCCAGTTTCCTGCTCTGGTGTTCGTCTTGTTTCTGTTCATAGCGTTCTCGAATCTCCACAGAGAACGCAGCATTCTGAAAATTCAAGAGAGCCCTGATCTCGTTCTTCACCGTCAAGATTCATTCACTTTTGTAAAGCCGAATCTTTTCACCCTGCAAAATCATGCTCCAGGGGTTTTGAATAGATTCAGTACATGCAACTCCACCAGAGAGTACAGTGGTCAGAAAATTCTGTGGACTGCCAGGACGCCGGAGTACGGTCACCGGAGAGAGAGTTTGGAGAGGTGTGATGTGTTTTCCGGCAAATGGGTGTTTGATAATGCTTCGTATCCGCTGTACAAGGAGTCGGACTGCCCTTACATGTCGGACCAGTTGGCCTGTCACAAGCATGGGAGGCCTGATTTGGGGTACCAGTACTGGAGATGGCAACCCCACAATTGCAACTTGAAGAGATGGAATGTGACTGAAATGTGGGAGAAATTGAGAGGCAAAAGGCTGATGTTTGTAGGGGATTCACTAAACAGAGGGCAGTGGATATCAATGGTCTGTTTGTTACAATCTGTGATTCCCGCAGATAAGAGATCAATGTCGCCAAATGCTGACCTTACCATTTTCCAAGCAGAGGAATATAATGCTACTGTGGAATTTCTCTGGGCTCCGCTTCTTGTAGAATCTAATTCTGATGACCCGGTGAATCACCGAACATCTGATAGGATAATCCGACCAGATTCAGTTCTTAAGCATGCATCAGTATGGGAGGATGCTGATATACTGATATTCAATTCCTACCTTTGGTGGAGACAAGGCCCAGTTAAGCTGTTATGGAGCGCTGAAGCAAACAGTGTTTGTGAAGAAGTAGACGGCCTTGGAGCCATGGAGTTGGCCATGGGGGCTTGGGCCGACTGGGCTTCCAAGGTCAATCCCCTCAAGAAGCGGGTCTTCTTCGTTACAACGTCTCCTACGCATCTCTGGAGTCGAGAGTGGAAACCCGAAAGTGAAGGTAATTGTTACAGTGAGAAATCCCCCATTGACTCGGAGGGCTACTGGGGAAGTGGTTCTGACCTGCCTACCATGAGCATGGTGGAGAAGGTCCTCAACAAGTTGAGTTCGAAGGTTTCCGTTATCAACATTACTCAATTATCTGAGTACCGGAAAGATGGCCACCCTTCCATATACCGCAAATTCTGGGAGCCACTGCGGCCTGAGCAATTGTCTAATCCCACGAGTTACTCCGATTGCATACACTGGTGCTTGCCTGGTGTACCCGATGTATGGAACGAGTTGCTATTCCAATTCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0070589 GO:0070592 GO:0071554 GO:0071704 GO:0071840 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

447

Amino Acids

51.94

Weight (kDa)

7.58

Isoelectric Point (pI)

51.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	107 - 161	5.9e-24	PMR5 N terminal Domain
PC-Esterase	PF13839	163 - 444	1.6e-81	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01620 AT5G01620 AT5G01620
fragaria_vesca	FvH4_6g10720 FvH4_6g10720 FvH4_6g10720 FvH4_6g10720
malus_domestica	MD04G1165100.v1.1 MD12G1179100.v1.1
prunus_persica	Prupe.6G284200_v2.0.a1
pyrus_communis	pycom04g14720 pycom12g16900
rosa_chinensis	RchiOBHm_Chr3g0461751
rosa_laevigata	RLG00000024903
rosa_multiflora	Rmu_sc0000619.1_g000010
rosa_roxburghii	Rroxscaffold_6G00417990
rosa_rugosa	Rorug03G0053200
rosa_samantha	Rh3AG111400 Rh3BG114400 Rh3CG116700 Rh3DG116400
rosa_wichuraiana	Rw3G009430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1110
Acc65I	GGTACC	1 cut(s)	447
AccB1I	GGYRCC	1 cut(s)	447
AccI	GTMKAC	1 cut(s)	897
AciI	CCGC	6 cut(s)	371, 609, 698, 973, 1216, 1237
AcoI	YGGCCR	2 cut(s)	921, 1198
AcsI	RAATTY	4 cut(s)	120, 267, 683, 1220
AcuI	CTGAAG	1 cut(s)	891
AcyI	GRCGYC	1 cut(s)	287
AfaI	GTAC	8 cut(s)	232, 254, 296, 377, 449, 455, 1187, 1302
AfeI	AGCGCT	1 cut(s)	868
AfiI	CCNNNNNNNGG	6 cut(s)	296, 345, 443, 661, 831, 1311
AflII	CTTAAG	1 cut(s)	776
AflIII	ACRYGT	1 cut(s)	399
AgsI	TTSAA	4 cut(s)	125, 220, 487, 820
AjnI	CCWGG	3 cut(s)	208, 281, 1294
AluBI	AGCT	1 cut(s)	856
AluI	AGCT	1 cut(s)	856
Alw26I	GTCTC	2 cut(s)	832, 999
Aor51HI	AGCGCT	1 cut(s)	868
AoxI	GGCC	8 cut(s)	414, 434, 844, 901, 921, 936, 1198, 1238
ApeKI	GCWGC	2 cut(s)	4, 108
ApoI	RAATTY	4 cut(s)	120, 267, 683, 1220
Asp700I	GAANNNNTTC	3 cut(s)	185, 224, 1093
Asp718I	GGTACC	1 cut(s)	447
AspLEI	GCGC	1 cut(s)	869
AspS9I	GGNCC	4 cut(s)	406, 845, 936, 1129
AsuC2I	CCSGG	1 cut(s)	728
AsuHPI	GGTGA	5 cut(s)	140, 181, 293, 728, 742
AsuII	TTCGAA	1 cut(s)	1148
AvaII	GGWCC	2 cut(s)	406, 1129
BalI	TGGCCA	2 cut(s)	923, 1200
BanI	GGYRCC	1 cut(s)	447
BanII	GRGCYC	2 cut(s)	134, 697
BauI	CACGAG	1 cut(s)	1261
BbsI	GAAGAC	1 cut(s)	970
BbvI	GCAGC	2 cut(s)	16, 120
BccI	CCATC	3 cut(s)	456, 486, 1190
BceAI	ACGGC	1 cut(s)	916
BciT130I	CCWGG	3 cut(s)	210, 283, 1296
BciVI	GTATCC	1 cut(s)	378
BcnI	CCSGG	1 cut(s)	728
BcoDI	GTCTC	2 cut(s)	832, 999
BfoI	RGCGCY	1 cut(s)	870
BfrI	CTTAAG	1 cut(s)	776
BfuAI	ACCTGC	1 cut(s)	1110
BfuI	GTATCC	1 cut(s)	378
BglI	GCCNNNNNGGC	3 cut(s)	929, 944, 1237
BisI	GCNGC	3 cut(s)	5, 109, 1238
BlsI	GCNGC	3 cut(s)	6, 110, 1239
BmcAI	AGTACT	1 cut(s)	455
Bme1390I	CCNGG	4 cut(s)	210, 283, 728, 1296
Bme18I	GGWCC	2 cut(s)	406, 1129
BmgT120I	GGNCC	4 cut(s)	406, 845, 936, 1129
BmiI	GGNNCC	4 cut(s)	449, 696, 910, 1230
BmrFI	CCNGG	4 cut(s)	210, 283, 728, 1296
BmrI	ACTGGG	4 cut(s)	37, 842, 952, 1093
BmsI	GCATC	3 cut(s)	790, 794, 1012
BmuI	ACTGGG	4 cut(s)	37, 842, 952, 1093
BpiI	GAAGAC	1 cut(s)	970
BpmI	CTGGAG	3 cut(s)	192, 478, 1030
Bpu10I	CCTNAGC	1 cut(s)	1242
Bpu14I	TTCGAA	1 cut(s)	1148
BpuEI	CTTGAG	1 cut(s)	950
BpuMI	CCSGG	1 cut(s)	728
BsaHI	GRCGYC	1 cut(s)	287
BsaJI	CCNNGG	5 cut(s)	209, 904, 912, 924, 951
BsaWI	WCCGGW	2 cut(s)	303, 1188
BsaXI	ACNNNNNCTCC	4 cut(s)	298, 328, 979, 1009
Bsc4I	CCNNNNNNNGG	6 cut(s)	296, 345, 443, 661, 831, 1311
Bse1I	ACTGG	8 cut(s)	43, 409, 451, 461, 848, 947, 1088, 1289
BseBI	CCWGG	3 cut(s)	210, 283, 1296
BseDI	CCNNGG	5 cut(s)	209, 904, 912, 924, 951
BseGI	GGATG	1 cut(s)	805
BseLI	CCNNNNNNNGG	6 cut(s)	296, 345, 443, 661, 831, 1311
BseMII	CTCAG	2 cut(s)	1173, 1233
BseNI	ACTGG	8 cut(s)	43, 409, 451, 461, 848, 947, 1088, 1289
BseXI	GCAGC	2 cut(s)	16, 120
BshFI	GGCC	8 cut(s)	416, 436, 846, 903, 923, 938, 1200, 1240
BshNI	GGYRCC	1 cut(s)	447
BsiSI	CCGG	5 cut(s)	290, 304, 339, 728, 1189
BslI	CCNNNNNNNGG	6 cut(s)	296, 345, 443, 661, 831, 1311
BsmAI	GTCTC	2 cut(s)	832, 999
BsmBI	CGTCTC	1 cut(s)	999
BsmI	GAATGC	1 cut(s)	111
BsnI	GGCC	8 cut(s)	416, 436, 846, 903, 923, 938, 1200, 1240
Bsp119I	TTCGAA	1 cut(s)	1148
Bsp1286I	GDGCHC	2 cut(s)	134, 697
Bsp1407I	TGTACA	1 cut(s)	375
Bsp143I	GATC	2 cut(s)	136, 620
Bsp19I	CCATGG	2 cut(s)	912, 924
BspACI	CCGC	6 cut(s)	371, 609, 698, 973, 1216, 1237
BspANI	GGCC	8 cut(s)	416, 436, 846, 903, 923, 938, 1200, 1240
BspCNI	CTCAG	2 cut(s)	1174, 1234
BspLI	GGNNCC	4 cut(s)	449, 696, 910, 1230
BspMI	ACCTGC	1 cut(s)	1110
BspT104I	TTCGAA	1 cut(s)	1148
BspT107I	GGYRCC	1 cut(s)	447
BspTI	CTTAAG	1 cut(s)	776
BsrGI	TGTACA	1 cut(s)	375
BsrI	ACTGG	8 cut(s)	43, 409, 451, 461, 848, 947, 1088, 1289
BssECI	CCNNGG	5 cut(s)	209, 904, 912, 924, 951
BssMI	GATC	2 cut(s)	136, 620
BssNI	GRCGYC	1 cut(s)	287
BssSI	CACGAG	1 cut(s)	1261
BssT1I	CCWWGG	4 cut(s)	904, 912, 924, 951
Bst2BI	CACGAG	1 cut(s)	1261
Bst2UI	CCWGG	3 cut(s)	210, 283, 1296
Bst4CI	ACNGT	6 cut(s)	152, 257, 299, 679, 881, 1052
Bst6I	CTCTTC	1 cut(s)	482
BstACI	GRCGYC	1 cut(s)	287
BstAFI	CTTAAG	1 cut(s)	776
BstAUI	TGTACA	1 cut(s)	375
BstBI	TTCGAA	1 cut(s)	1148
BstC8I	GCNNGC	2 cut(s)	783, 1292
BstDEI	CTNAG	2 cut(s)	1182, 1242
BstDSI	CCRYGG	2 cut(s)	912, 924
BstEII	GGTNACC	1 cut(s)	299
BstF5I	GGATG	1 cut(s)	805
BstH2I	RGCGCY	1 cut(s)	870
BstHHI	GCGC	1 cut(s)	869
BstKTI	GATC	2 cut(s)	139, 623
BstMAI	GTCTC	2 cut(s)	832, 999
BstMBI	GATC	2 cut(s)	136, 620
BstMWI	GCNNNNNNNGC	6 cut(s)	10, 433, 929, 944, 1237, 1243
BstNI	CCWGG	3 cut(s)	210, 283, 1296
BstNSI	RCATGY	3 cut(s)	237, 403, 785
BstPI	GGTNACC	1 cut(s)	299
BstSCI	CCNGG	4 cut(s)	208, 281, 726, 1294
BstV1I	GCAGC	2 cut(s)	16, 120
BstV2I	GAAGAC	1 cut(s)	970
BstXI	CCANNNNNNTGG	2 cut(s)	919, 1117
BsuI	GTATCC	1 cut(s)	378
BsuRI	GGCC	8 cut(s)	416, 436, 846, 903, 923, 938, 1200, 1240
BtgI	CCRYGG	2 cut(s)	912, 924
BtsCI	GGATG	1 cut(s)	805
BtsI	GCAGTG	2 cut(s)	575, 1232
BtsIMutI	CAGTG	6 cut(s)	262, 575, 886, 1057, 1232, 1282
BveI	ACCTGC	1 cut(s)	1110
Cac8I	GCNNGC	2 cut(s)	783, 1292
CfoI	GCGC	1 cut(s)	869
Cfr13I	GGNCC	4 cut(s)	406, 845, 936, 1129
CseI	GACGC	1 cut(s)	295
Csp6I	GTAC	8 cut(s)	231, 253, 295, 376, 448, 454, 1186, 1301
CviAII	CATG	9 cut(s)	203, 234, 400, 428, 782, 913, 925, 1111, 1117
CviQI	GTAC	8 cut(s)	231, 253, 295, 376, 448, 454, 1186, 1301
DdeI	CTNAG	2 cut(s)	1182, 1242
DpnI	GATC	2 cut(s)	138, 622
DpnII	GATC	2 cut(s)	136, 620
EaeI	YGGCCR	2 cut(s)	921, 1198
Eam1104I	CTCTTC	1 cut(s)	482
EarI	CTCTTC	1 cut(s)	482
Eco130I	CCWWGG	4 cut(s)	904, 912, 924, 951
Eco147I	AGGCCT	1 cut(s)	436
Eco24I	GRGCYC	2 cut(s)	134, 697
Eco32I	GATATC	1 cut(s)	576
Eco47I	GGWCC	2 cut(s)	406, 1129
Eco47III	AGCGCT	1 cut(s)	868
Eco57I	CTGAAG	1 cut(s)	891
Eco91I	GGTNACC	1 cut(s)	299
EcoO109I	RGGNCCY	1 cut(s)	1129
EcoO65I	GGTNACC	1 cut(s)	299
EcoRII	CCWGG	3 cut(s)	208, 281, 1294
EcoRV	GATATC	1 cut(s)	576
EcoT14I	CCWWGG	4 cut(s)	904, 912, 924, 951
EcoT22I	ATGCAT	1 cut(s)	787
EcoT38I	GRGCYC	2 cut(s)	134, 697
ErhI	CCWWGG	4 cut(s)	904, 912, 924, 951
Esp3I	CGTCTC	1 cut(s)	999
FaeI	CATG	9 cut(s)	206, 237, 403, 431, 785, 916, 928, 1114, 1120
FalI	AAGNNNNNCTT	2 cut(s)	962, 994
FatI	CATG	9 cut(s)	202, 233, 399, 427, 781, 912, 924, 1110, 1116
FauI	CCCGC	2 cut(s)	616, 966
FblI	GTMKAC	1 cut(s)	897
Fnu4HI	GCNGC	3 cut(s)	5, 109, 1238
FokI	GGATG	1 cut(s)	812
FriOI	GRGCYC	2 cut(s)	134, 697
Fsp4HI	GCNGC	3 cut(s)	5, 109, 1238
GlaI	GCGC	1 cut(s)	868
GluI	GCNGC	3 cut(s)	5, 109, 1238
GsuI	CTGGAG	3 cut(s)	192, 478, 1030
HaeII	RGCGCY	1 cut(s)	870
HaeIII	GGCC	8 cut(s)	416, 436, 846, 903, 923, 938, 1200, 1240
HapII	CCGG	5 cut(s)	290, 304, 339, 728, 1189
HgaI	GACGC	1 cut(s)	295
HhaI	GCGC	1 cut(s)	869
Hin1I	GRCGYC	1 cut(s)	287
Hin1II	CATG	9 cut(s)	206, 237, 403, 431, 785, 916, 928, 1114, 1120
Hin6I	GCGC	1 cut(s)	867
HinP1I	GCGC	1 cut(s)	867
HpaII	CCGG	5 cut(s)	290, 304, 339, 728, 1189
HphI	GGTGA	5 cut(s)	140, 181, 293, 728, 742
Hpy166II	GTNNAC	3 cut(s)	276, 898, 1301
Hpy188III	TCNNGA	6 cut(s)	88, 125, 155, 967, 1009, 1016
Hpy8I	GTNNAC	3 cut(s)	276, 898, 1301
HpyAV	CCTTC	4 cut(s)	1031, 1120, 1144, 1215
HpyCH4III	ACNGT	6 cut(s)	152, 257, 299, 679, 881, 1052
HpyCH4IV	ACGT	1 cut(s)	992
HpyCH4V	TGCA	6 cut(s)	4, 196, 237, 480, 785, 1278
HpyF10VI	GCNNNNNNNGC	6 cut(s)	10, 433, 929, 944, 1237, 1243
HpyF3I	CTNAG	2 cut(s)	1182, 1242
HpySE526I	ACGT	1 cut(s)	992
Hsp92I	GRCGYC	1 cut(s)	287
Hsp92II	CATG	9 cut(s)	206, 237, 403, 431, 785, 916, 928, 1114, 1120
HspAI	GCGC	1 cut(s)	867
KpnI	GGTACC	1 cut(s)	451
Kzo9I	GATC	2 cut(s)	136, 620
LmnI	GCTCC	5 cut(s)	211, 700, 864, 908, 1228
Lsp1109I	GCAGC	2 cut(s)	16, 120
LweI	GCATC	3 cut(s)	790, 794, 1012
MaeII	ACGT	1 cut(s)	992
MaeIII	GTNAC	7 cut(s)	299, 419, 499, 591, 985, 1046, 1265
MalI	GATC	2 cut(s)	138, 622
MboI	GATC	2 cut(s)	136, 620
MboII	GAAGA	6 cut(s)	46, 137, 499, 902, 970, 973
MfeI	CAATTG	2 cut(s)	475, 1247
MhlI	GDGCHC	2 cut(s)	134, 697
MlsI	TGGCCA	2 cut(s)	923, 1200
MluNI	TGGCCA	2 cut(s)	923, 1200
MlyI	GAGTC	3 cut(s)	392, 1021, 1064
MmeI	TCCRAC	3 cut(s)	366, 384, 784
Mox20I	TGGCCA	2 cut(s)	923, 1200
Mph1103I	ATGCAT	1 cut(s)	787
MroXI	GAANNNNTTC	3 cut(s)	185, 224, 1093
MscI	TGGCCA	2 cut(s)	923, 1200
MseI	TTAA	2 cut(s)	777, 852
MslI	CAYNNNNRTG	3 cut(s)	426, 790, 1115
Msp20I	TGGCCA	2 cut(s)	923, 1200
MspA1I	CMGCKG	1 cut(s)	373
MspCI	CTTAAG	1 cut(s)	776
MspI	CCGG	5 cut(s)	290, 304, 339, 728, 1189
MspR9I	CCNGG	4 cut(s)	210, 283, 728, 1296
MunI	CAATTG	2 cut(s)	475, 1247
Mva1269I	GAATGC	1 cut(s)	111
MvaI	CCWGG	3 cut(s)	210, 283, 1296
MwoI	GCNNNNNNNGC	6 cut(s)	10, 433, 929, 944, 1237, 1243
NciI	CCSGG	1 cut(s)	728
NcoI	CCATGG	2 cut(s)	912, 924
NdeII	GATC	2 cut(s)	136, 620
NlaIII	CATG	9 cut(s)	206, 237, 403, 431, 785, 916, 928, 1114, 1120
NlaIV	GGNNCC	4 cut(s)	449, 696, 910, 1230
NmuCI	GTSAC	3 cut(s)	299, 419, 499
NsiI	ATGCAT	1 cut(s)	787
NspI	RCATGY	3 cut(s)	237, 403, 785
NspV	TTCGAA	1 cut(s)	1148
PaeI	GCATGC	1 cut(s)	785
PceI	AGGCCT	1 cut(s)	436
PciI	ACATGT	1 cut(s)	399
PctI	GAATGC	1 cut(s)	111
PdmI	GAANNNNTTC	3 cut(s)	185, 224, 1093
PfeI	GAWTC	9 cut(s)	91, 158, 181, 225, 551, 604, 710, 733, 767
PkrI	GCNGC	3 cut(s)	6, 110, 1239
PleI	GAGTC	3 cut(s)	391, 1020, 1064
PpsI	GAGTC	3 cut(s)	391, 1020, 1064
PpuMI	RGGWCCY	1 cut(s)	1129
PscI	ACATGT	1 cut(s)	399
Psp5II	RGGWCCY	1 cut(s)	1129
Psp6I	CCWGG	3 cut(s)	208, 281, 1294
PspEI	GGTNACC	1 cut(s)	299
PspGI	CCWGG	3 cut(s)	208, 281, 1294
PspN4I	GGNNCC	4 cut(s)	449, 696, 910, 1230
PspPI	GGNCC	4 cut(s)	406, 845, 936, 1129
PspPPI	RGGWCCY	1 cut(s)	1129
RsaI	GTAC	8 cut(s)	232, 254, 296, 377, 449, 455, 1187, 1302
RsaNI	GTAC	8 cut(s)	231, 253, 295, 376, 448, 454, 1186, 1301
RseI	CAYNNNNRTG	3 cut(s)	426, 790, 1115
SaqAI	TTAA	2 cut(s)	777, 852
SatI	GCNGC	3 cut(s)	5, 109, 1238
Sau3AI	GATC	2 cut(s)	136, 620
Sau96I	GGNCC	4 cut(s)	406, 845, 936, 1129
ScaI	AGTACT	1 cut(s)	455
SchI	GAGTC	3 cut(s)	392, 1021, 1064
ScrFI	CCNGG	4 cut(s)	210, 283, 728, 1296
SduI	GDGCHC	2 cut(s)	134, 697
SfaNI	GCATC	3 cut(s)	790, 794, 1012
SfuI	TTCGAA	1 cut(s)	1148
SinI	GGWCC	2 cut(s)	406, 1129
SmiMI	CAYNNNNRTG	3 cut(s)	426, 790, 1115
SmlI	CTYRAG	2 cut(s)	776, 965
SmoI	CTYRAG	2 cut(s)	776, 965
SphI	GCATGC	1 cut(s)	785
SseBI	AGGCCT	1 cut(s)	436
SsiI	CCGC	6 cut(s)	371, 609, 698, 973, 1216, 1237
StuI	AGGCCT	1 cut(s)	436
StyD4I	CCNGG	4 cut(s)	208, 281, 726, 1294
StyI	CCWWGG	4 cut(s)	904, 912, 924, 951
TaaI	ACNGT	6 cut(s)	152, 257, 299, 679, 881, 1052
TaiI	ACGT	1 cut(s)	995
TaqI	TCGA	4 cut(s)	26, 89, 1015, 1148
TatI	WGTACW	4 cut(s)	230, 252, 375, 453
TauI	GCSGC	1 cut(s)	1240
TfiI	GAWTC	9 cut(s)	91, 158, 181, 225, 551, 604, 710, 733, 767
Tru1I	TTAA	2 cut(s)	777, 852
Tru9I	TTAA	2 cut(s)	777, 852
TscAI	CASTG	6 cut(s)	262, 575, 886, 1057, 1239, 1289
TseFI	GTSAC	3 cut(s)	299, 419, 499
TseI	GCWGC	2 cut(s)	4, 108
Tsp45I	GTSAC	3 cut(s)	299, 419, 499
TspDTI	ATGAA	2 cut(s)	67, 150
TspGWI	ACGGA	1 cut(s)	1147
TspRI	CASTG	6 cut(s)	262, 575, 886, 1057, 1239, 1289
Vha464I	CTTAAG	1 cut(s)	776
VpaK11BI	GGWCC	2 cut(s)	406, 1129
XapI	RAATTY	4 cut(s)	120, 267, 683, 1220
XceI	RCATGY	3 cut(s)	237, 403, 785
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1117
XmiI	GTMKAC	1 cut(s)	897
XmnI	GAANNNNTTC	3 cut(s)	185, 224, 1093
ZrmI	AGTACT	1 cut(s)	455
Zsp2I	ATGCAT	1 cut(s)	787

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.