Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009175

Physical Location & Seq

Genomic Coordinates Forward (+)

25953281 .. 25956702

Gene Structure 3422 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1179100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGCAGCAGTGGCAGAGGTGGAAGTGGAACAAGAAGAAAACCCATGTTCCTGCGCTGGTGTTCTTCTTCTTTCTGTTCATAGTTTTCTCGAATCTCCACAGCCAACGCAGAATTCTGCAAATTCAAGAGAGCCCTGATCGCGTTTATCACCGTCAAGATTCAGTCACTTTTGTAAAGCCAAATCTTTTCACCCAGCAAAACCATGCTCCAGAGGTTTTGGATAGATTCAGTACATGCAACTCCACCAGAGAGTACAGTGGCCAGAAAATTCTGTGGACTGACCGGACGCCGGAGTACGGTCACCGGAGAGAGAGTTTGGAGAGGTGTGATGTGTTTTCCGGCAAATGGGTGTTTGATAATGCGTCGTATCCGCTGTACAAGGAGTCGGAGTGCCCTTACATGTCGGACCAGTTGGCCTGTCACAAGCATGGGAGGCCTGATTTGGGGTACCAGTACTGGAGATGGCAACCCCACAATTGCAACTTGAAGAGATGGAATGTGACTGAAATGTGGGAGAAGTTGAGAGGCAAAAGGCTAATGTTTGTAGGGGATTCATTAAACCGAGGGCAATGGATATCAATGGTCTGTTTGTTACAATCTGTGATTCCTTCAGATAGGAGATCAATGTCACCGAACGCTGAACTTACTATTTTCAAAGCAGAGGAATATAATGCAACAGTGGAATTTCTCTGGGCTCCGCTTCTTGTAGAATCTAATTCTGATGATCCGGTGAATCACCGAACATCCGATCGGATAATCCGACCAGATTCAGTTCTTAAGCATGCATCAATATGGGAGAATGCTGATATATTGATATTCAATTCCTACCTGTGGTGGAGACAAGGCCCGGTTAAGCTGCTATGGAGCGCGGAAGCAAACGGTGTTTGTGAAGAAGTAGACGGCCTTGGAGCCATGGAGTTGGCCATGGGGGCTTGGGCAGACTGGGCTTCTAAGGTCAATCCCCTCAAGAAGCAGGTCTTCTTCGTCACAATGTCTCCTACACATCTCTGGAGTCGAGAATGGAAACAAGAAAGTGAAGGTAATTGTTACAGTGAGAAATCCCCCATTGACTTGGAGGGCTACTGGGGAAGTGGTTCTGACCTGCCTACCATGAGCATGGTGGAGACGGTCCTCAGCAAGTTGAGTTCCAAGGTTTCTGTCATCAACATTACGCAATTATCGGAGTACCGGAAAGATGGTCACCCTTCCATTTACCGCAAATTCTGGGAGCCACTGAAGCCAGAACAATTGTCTAAGCCCACAAGTTACTCGGATTGCATACACTGGTGCTTGCCTGGTGTACCTGACGTATGGAACGAGTTGCTATTCCAATTCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0070589 GO:0070592 GO:0071554 GO:0071704 GO:0071840 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

447

Amino Acids

52.46

Weight (kDa)

7.19

Isoelectric Point (pI)

52.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	107 - 161	3.5e-24	PMR5 N terminal Domain
PC-Esterase	PF13839	163 - 444	2e-81	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009175)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01620 AT5G01620 AT5G01620
fragaria_vesca	FvH4_6g10720 FvH4_6g10720 FvH4_6g10720 FvH4_6g10720
malus_domestica	MD04G1165100.v1.1 MD12G1179100.v1.1
prunus_persica	Prupe.6G284200_v2.0.a1
pyrus_communis	pycom04g14720 pycom12g16900
rosa_chinensis	RchiOBHm_Chr3g0461751
rosa_laevigata	RLG00000024903
rosa_multiflora	Rmu_sc0000619.1_g000010
rosa_roxburghii	Rroxscaffold_6G00417990
rosa_rugosa	Rorug03G0053200
rosa_samantha	Rh3AG111400 Rh3BG114400 Rh3CG116700 Rh3DG116400
rosa_wichuraiana	Rw3G009430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	964, 1110
Acc65I	GGTACC	1 cut(s)	447
AccB1I	GGYRCC	1 cut(s)	447
AccI	GTMKAC	1 cut(s)	897
AccII	CGCG	2 cut(s)	141, 869
AciI	CCGC	4 cut(s)	371, 698, 869, 1216
AclWI	GGATC	1 cut(s)	719
AcoI	YGGCCR	2 cut(s)	259, 921
AcsI	RAATTY	5 cut(s)	111, 120, 267, 683, 1220
AcuI	CTGAAG	2 cut(s)	594, 1256
AcyI	GRCGYC	1 cut(s)	287
AfaI	GTAC	8 cut(s)	232, 254, 296, 377, 449, 455, 1187, 1302
AfiI	CCNNNNNNNGG	5 cut(s)	289, 296, 345, 443, 831
AflII	CTTAAG	1 cut(s)	776
AflIII	ACRYGT	1 cut(s)	399
AgsI	TTSAA	4 cut(s)	125, 487, 655, 820
AjnI	CCWGG	1 cut(s)	1294
AjuI	GAANNNNNNNTTGG	2 cut(s)	96, 128
AluBI	AGCT	1 cut(s)	856
AluI	AGCT	1 cut(s)	856
Alw26I	GTCTC	3 cut(s)	832, 999, 1118
AlwI	GGATC	1 cut(s)	719
AoxI	GGCC	6 cut(s)	259, 414, 434, 844, 901, 921
ApeKI	GCWGC	2 cut(s)	4, 856
ApoI	RAATTY	5 cut(s)	111, 120, 267, 683, 1220
Asp700I	GAANNNNTTC	1 cut(s)	1093
Asp718I	GGTACC	1 cut(s)	447
AspLEI	GCGC	2 cut(s)	55, 869
AspS9I	GGNCC	3 cut(s)	406, 845, 1129
AsuC2I	CCSGG	1 cut(s)	848
AsuHPI	GGTGA	7 cut(s)	140, 181, 293, 621, 728, 742, 1193
AvaII	GGWCC	2 cut(s)	406, 1129
BaeGI	GKGCMC	1 cut(s)	395
BalI	TGGCCA	2 cut(s)	261, 923
BanI	GGYRCC	1 cut(s)	447
BanII	GRGCYC	2 cut(s)	134, 697
BbsI	GAAGAC	1 cut(s)	970
BbvCI	CCTCAGC	1 cut(s)	1133
BbvI	GCAGC	2 cut(s)	16, 843
BccI	CCATC	3 cut(s)	456, 486, 1190
BceAI	ACGGC	1 cut(s)	916
BciT130I	CCWGG	1 cut(s)	1296
BciVI	GTATCC	1 cut(s)	378
BcnI	CCSGG	1 cut(s)	848
BcoDI	GTCTC	3 cut(s)	832, 999, 1118
BfrI	CTTAAG	1 cut(s)	776
BfuAI	ACCTGC	2 cut(s)	964, 1110
BfuI	GTATCC	1 cut(s)	378
BglI	GCCNNNNNGGC	1 cut(s)	929
BisI	GCNGC	2 cut(s)	5, 857
BlsI	GCNGC	2 cut(s)	6, 858
BmcAI	AGTACT	1 cut(s)	455
Bme1390I	CCNGG	2 cut(s)	848, 1296
Bme18I	GGWCC	2 cut(s)	406, 1129
BmgT120I	GGNCC	3 cut(s)	406, 845, 1129
BmiI	GGNNCC	4 cut(s)	449, 696, 910, 1230
BmrFI	CCNGG	2 cut(s)	848, 1296
BmrI	ACTGGG	2 cut(s)	952, 1093
BmsI	GCATC	1 cut(s)	794
BmuI	ACTGGG	2 cut(s)	952, 1093
BpiI	GAAGAC	1 cut(s)	970
BpmI	CTGGAG	3 cut(s)	192, 478, 1030
Bpu10I	CCTNAGC	1 cut(s)	1133
BpuEI	CTTGAG	1 cut(s)	950
BpuMI	CCSGG	1 cut(s)	848
BsaHI	GRCGYC	1 cut(s)	287
BsaJI	CCNNGG	5 cut(s)	562, 904, 912, 924, 1149
BsaWI	WCCGGW	4 cut(s)	282, 303, 727, 1188
BsaXI	ACNNNNNCTCC	6 cut(s)	298, 328, 374, 404, 979, 1009
Bsc4I	CCNNNNNNNGG	5 cut(s)	289, 296, 345, 443, 831
Bse1I	ACTGG	6 cut(s)	409, 451, 461, 947, 1088, 1289
Bse3DI	GCAATG	1 cut(s)	575
BseBI	CCWGG	1 cut(s)	1296
BseDI	CCNNGG	5 cut(s)	562, 904, 912, 924, 1149
BseGI	GGATG	1 cut(s)	743
BseLI	CCNNNNNNNGG	5 cut(s)	289, 296, 345, 443, 831
BseMI	GCAATG	1 cut(s)	575
BseMII	CTCAG	1 cut(s)	1147
BseNI	ACTGG	6 cut(s)	409, 451, 461, 947, 1088, 1289
BseSI	GKGCMC	1 cut(s)	395
BseXI	GCAGC	2 cut(s)	16, 843
BseYI	CCCAGC	1 cut(s)	192
Bsh1236I	CGCG	2 cut(s)	141, 869
Bsh1285I	CGRYCG	1 cut(s)	751
BshFI	GGCC	6 cut(s)	261, 416, 436, 846, 903, 923
BshNI	GGYRCC	1 cut(s)	447
BsiEI	CGRYCG	1 cut(s)	751
BsiSI	CCGG	7 cut(s)	283, 290, 304, 339, 728, 848, 1189
BslI	CCNNNNNNNGG	5 cut(s)	289, 296, 345, 443, 831
BsmAI	GTCTC	3 cut(s)	832, 999, 1118
BsmBI	CGTCTC	1 cut(s)	1118
BsmI	GAATGC	1 cut(s)	805
BsnI	GGCC	6 cut(s)	261, 416, 436, 846, 903, 923
Bsp1286I	GDGCHC	3 cut(s)	134, 395, 697
Bsp1407I	TGTACA	1 cut(s)	375
Bsp143I	GATC	4 cut(s)	136, 620, 724, 748
Bsp19I	CCATGG	2 cut(s)	912, 924
BspACI	CCGC	4 cut(s)	371, 698, 869, 1216
BspANI	GGCC	6 cut(s)	261, 416, 436, 846, 903, 923
BspCNI	CTCAG	1 cut(s)	1146
BspFNI	CGCG	2 cut(s)	141, 869
BspLI	GGNNCC	4 cut(s)	449, 696, 910, 1230
BspMI	ACCTGC	2 cut(s)	964, 1110
BspPI	GGATC	1 cut(s)	719
BspT107I	GGYRCC	1 cut(s)	447
BspTI	CTTAAG	1 cut(s)	776
BsrDI	GCAATG	1 cut(s)	575
BsrGI	TGTACA	1 cut(s)	375
BsrI	ACTGG	6 cut(s)	409, 451, 461, 947, 1088, 1289
BssECI	CCNNGG	5 cut(s)	562, 904, 912, 924, 1149
BssMI	GATC	4 cut(s)	136, 620, 724, 748
BssNI	GRCGYC	1 cut(s)	287
BssT1I	CCWWGG	4 cut(s)	904, 912, 924, 1149
Bst2UI	CCWGG	1 cut(s)	1296
Bst4CI	ACNGT	7 cut(s)	152, 257, 299, 679, 881, 1052, 1129
Bst6I	CTCTTC	1 cut(s)	482
BstACI	GRCGYC	1 cut(s)	287
BstAFI	CTTAAG	1 cut(s)	776
BstAUI	TGTACA	1 cut(s)	375
BstC8I	GCNNGC	2 cut(s)	783, 1292
BstDEI	CTNAG	3 cut(s)	951, 1133, 1254
BstDSI	CCRYGG	2 cut(s)	912, 924
BstEII	GGTNACC	2 cut(s)	299, 1199
BstF5I	GGATG	1 cut(s)	743
BstFNI	CGCG	2 cut(s)	141, 869
BstHHI	GCGC	2 cut(s)	55, 869
BstKTI	GATC	4 cut(s)	139, 623, 727, 751
BstMAI	GTCTC	3 cut(s)	832, 999, 1118
BstMBI	GATC	4 cut(s)	136, 620, 724, 748
BstMCI	CGRYCG	1 cut(s)	751
BstMWI	GCNNNNNNNGC	6 cut(s)	10, 138, 433, 929, 944, 1237
BstNI	CCWGG	1 cut(s)	1296
BstNSI	RCATGY	3 cut(s)	237, 403, 785
BstPI	GGTNACC	2 cut(s)	299, 1199
BstSCI	CCNGG	2 cut(s)	846, 1294
BstSLI	GKGCMC	1 cut(s)	395
BstUI	CGCG	2 cut(s)	141, 869
BstV1I	GCAGC	2 cut(s)	16, 843
BstV2I	GAAGAC	1 cut(s)	970
BstXI	CCANNNNNNTGG	3 cut(s)	919, 1072, 1117
BsuI	GTATCC	1 cut(s)	378
BsuRI	GGCC	6 cut(s)	261, 416, 436, 846, 903, 923
BtgI	CCRYGG	2 cut(s)	912, 924
BtsCI	GGATG	1 cut(s)	743
BtsI	GCAGTG	1 cut(s)	14
BtsIMutI	CAGTG	6 cut(s)	14, 262, 684, 1057, 1232, 1282
BveI	ACCTGC	2 cut(s)	964, 1110
Cac8I	GCNNGC	2 cut(s)	783, 1292
CfoI	GCGC	2 cut(s)	55, 869
Cfr13I	GGNCC	3 cut(s)	406, 845, 1129
CseI	GACGC	2 cut(s)	295, 351
Csp6I	GTAC	8 cut(s)	231, 253, 295, 376, 448, 454, 1186, 1301
CviQI	GTAC	8 cut(s)	231, 253, 295, 376, 448, 454, 1186, 1301
DdeI	CTNAG	3 cut(s)	951, 1133, 1254
DpnI	GATC	4 cut(s)	138, 622, 726, 750
DpnII	GATC	4 cut(s)	136, 620, 724, 748
EaeI	YGGCCR	2 cut(s)	259, 921
Eam1104I	CTCTTC	1 cut(s)	482
EarI	CTCTTC	1 cut(s)	482
Eco130I	CCWWGG	4 cut(s)	904, 912, 924, 1149
Eco147I	AGGCCT	1 cut(s)	436
Eco24I	GRGCYC	2 cut(s)	134, 697
Eco32I	GATATC	1 cut(s)	576
Eco47I	GGWCC	2 cut(s)	406, 1129
Eco57I	CTGAAG	2 cut(s)	594, 1256
Eco91I	GGTNACC	2 cut(s)	299, 1199
EcoO65I	GGTNACC	2 cut(s)	299, 1199
EcoRI	GAATTC	1 cut(s)	111
EcoRII	CCWGG	1 cut(s)	1294
EcoRV	GATATC	1 cut(s)	576
EcoT14I	CCWWGG	4 cut(s)	904, 912, 924, 1149
EcoT22I	ATGCAT	1 cut(s)	787
EcoT38I	GRGCYC	2 cut(s)	134, 697
ErhI	CCWWGG	4 cut(s)	904, 912, 924, 1149
Esp3I	CGTCTC	1 cut(s)	1118
FalI	AAGNNNNNCTT	2 cut(s)	962, 994
FblI	GTMKAC	1 cut(s)	897
Fnu4HI	GCNGC	2 cut(s)	5, 857
FokI	GGATG	1 cut(s)	730
FriOI	GRGCYC	2 cut(s)	134, 697
Fsp4HI	GCNGC	2 cut(s)	5, 857
GlaI	GCGC	2 cut(s)	54, 868
GluI	GCNGC	2 cut(s)	5, 857
GsaI	CCCAGC	1 cut(s)	196
GsuI	CTGGAG	3 cut(s)	192, 478, 1030
HaeIII	GGCC	6 cut(s)	261, 416, 436, 846, 903, 923
HapII	CCGG	7 cut(s)	283, 290, 304, 339, 728, 848, 1189
HgaI	GACGC	2 cut(s)	295, 351
HhaI	GCGC	2 cut(s)	55, 869
Hin1I	GRCGYC	1 cut(s)	287
Hin6I	GCGC	2 cut(s)	53, 867
HinP1I	GCGC	2 cut(s)	53, 867
HpaII	CCGG	7 cut(s)	283, 290, 304, 339, 728, 848, 1189
HphI	GGTGA	7 cut(s)	140, 181, 293, 621, 728, 742, 1193
Hpy166II	GTNNAC	3 cut(s)	276, 898, 1301
Hpy188III	TCNNGA	7 cut(s)	88, 125, 155, 209, 967, 1009, 1016
Hpy8I	GTNNAC	3 cut(s)	276, 898, 1301
Hpy99I	CGWCG	1 cut(s)	367
HpyAV	CCTTC	3 cut(s)	618, 1031, 1215
HpyCH4III	ACNGT	7 cut(s)	152, 257, 299, 679, 881, 1052, 1129
HpyCH4IV	ACGT	1 cut(s)	1308
HpyCH4V	TGCA	7 cut(s)	4, 118, 237, 480, 674, 785, 1278
HpyF10VI	GCNNNNNNNGC	6 cut(s)	10, 138, 433, 929, 944, 1237
HpyF3I	CTNAG	3 cut(s)	951, 1133, 1254
HpySE526I	ACGT	1 cut(s)	1308
Hsp92I	GRCGYC	1 cut(s)	287
HspAI	GCGC	2 cut(s)	53, 867
KpnI	GGTACC	1 cut(s)	451
Kzo9I	GATC	4 cut(s)	136, 620, 724, 748
LmnI	GCTCC	5 cut(s)	211, 700, 864, 908, 1228
Lsp1109I	GCAGC	2 cut(s)	16, 843
LweI	GCATC	1 cut(s)	794
MaeII	ACGT	1 cut(s)	1308
MalI	GATC	4 cut(s)	138, 622, 726, 750
MboI	GATC	4 cut(s)	136, 620, 724, 748
MboII	GAAGA	7 cut(s)	46, 55, 58, 499, 902, 970, 973
MfeI	CAATTG	2 cut(s)	475, 1247
MhlI	GDGCHC	3 cut(s)	134, 395, 697
MlsI	TGGCCA	2 cut(s)	261, 923
MluNI	TGGCCA	2 cut(s)	261, 923
MlyI	GAGTC	2 cut(s)	392, 1021
MmeI	TCCRAC	3 cut(s)	366, 384, 784
Mox20I	TGGCCA	2 cut(s)	261, 923
Mph1103I	ATGCAT	1 cut(s)	787
MroXI	GAANNNNTTC	1 cut(s)	1093
MscI	TGGCCA	2 cut(s)	261, 923
MseI	TTAA	3 cut(s)	557, 777, 852
MslI	CAYNNNNRTG	3 cut(s)	426, 790, 1115
Msp20I	TGGCCA	2 cut(s)	261, 923
MspA1I	CMGCKG	1 cut(s)	373
MspCI	CTTAAG	1 cut(s)	776
MspI	CCGG	7 cut(s)	283, 290, 304, 339, 728, 848, 1189
MspR9I	CCNGG	2 cut(s)	848, 1296
MunI	CAATTG	2 cut(s)	475, 1247
Mva1269I	GAATGC	1 cut(s)	805
MvaI	CCWGG	1 cut(s)	1296
MvnI	CGCG	2 cut(s)	141, 869
MwoI	GCNNNNNNNGC	6 cut(s)	10, 138, 433, 929, 944, 1237
NciI	CCSGG	1 cut(s)	848
NcoI	CCATGG	2 cut(s)	912, 924
NdeII	GATC	4 cut(s)	136, 620, 724, 748
NlaIV	GGNNCC	4 cut(s)	449, 696, 910, 1230
NmuCI	GTSAC	7 cut(s)	163, 299, 419, 499, 627, 985, 1199
NsiI	ATGCAT	1 cut(s)	787
NspI	RCATGY	3 cut(s)	237, 403, 785
PaeI	GCATGC	1 cut(s)	785
PceI	AGGCCT	1 cut(s)	436
PciI	ACATGT	1 cut(s)	399
PcsI	WCGNNNNNNNCGW	2 cut(s)	757, 1314
PctI	GAATGC	1 cut(s)	805
PdmI	GAANNNNTTC	1 cut(s)	1093
PfeI	GAWTC	8 cut(s)	91, 158, 225, 551, 604, 710, 733, 767
PkrI	GCNGC	2 cut(s)	6, 858
Ple19I	CGATCG	1 cut(s)	751
PleI	GAGTC	2 cut(s)	391, 1020
PpsI	GAGTC	2 cut(s)	391, 1020
PscI	ACATGT	1 cut(s)	399
Psp6I	CCWGG	1 cut(s)	1294
PspEI	GGTNACC	2 cut(s)	299, 1199
PspFI	CCCAGC	1 cut(s)	192
PspGI	CCWGG	1 cut(s)	1294
PspN4I	GGNNCC	4 cut(s)	449, 696, 910, 1230
PspPI	GGNCC	3 cut(s)	406, 845, 1129
PvuI	CGATCG	1 cut(s)	751
RsaI	GTAC	8 cut(s)	232, 254, 296, 377, 449, 455, 1187, 1302
RsaNI	GTAC	8 cut(s)	231, 253, 295, 376, 448, 454, 1186, 1301
RseI	CAYNNNNRTG	3 cut(s)	426, 790, 1115
SaqAI	TTAA	3 cut(s)	557, 777, 852
SatI	GCNGC	2 cut(s)	5, 857
Sau3AI	GATC	4 cut(s)	136, 620, 724, 748
Sau96I	GGNCC	3 cut(s)	406, 845, 1129
ScaI	AGTACT	1 cut(s)	455
SchI	GAGTC	2 cut(s)	392, 1021
ScrFI	CCNGG	2 cut(s)	848, 1296
SduI	GDGCHC	3 cut(s)	134, 395, 697
SfaNI	GCATC	1 cut(s)	794
SinI	GGWCC	2 cut(s)	406, 1129
SmiMI	CAYNNNNRTG	3 cut(s)	426, 790, 1115
SmlI	CTYRAG	2 cut(s)	776, 965
SmoI	CTYRAG	2 cut(s)	776, 965
SphI	GCATGC	1 cut(s)	785
SseBI	AGGCCT	1 cut(s)	436
SsiI	CCGC	4 cut(s)	371, 698, 869, 1216
StuI	AGGCCT	1 cut(s)	436
StyD4I	CCNGG	2 cut(s)	846, 1294
StyI	CCWWGG	4 cut(s)	904, 912, 924, 1149
TaaI	ACNGT	7 cut(s)	152, 257, 299, 679, 881, 1052, 1129
TaiI	ACGT	1 cut(s)	1311
TaqI	TCGA	2 cut(s)	89, 1015
TatI	WGTACW	4 cut(s)	230, 252, 375, 453
TfiI	GAWTC	8 cut(s)	91, 158, 225, 551, 604, 710, 733, 767
Tru1I	TTAA	3 cut(s)	557, 777, 852
Tru9I	TTAA	3 cut(s)	557, 777, 852
TscAI	CASTG	6 cut(s)	14, 262, 684, 1057, 1239, 1289
TseFI	GTSAC	7 cut(s)	163, 299, 419, 499, 627, 985, 1199
TseI	GCWGC	2 cut(s)	4, 856
Tsp45I	GTSAC	7 cut(s)	163, 299, 419, 499, 627, 985, 1199
TspDTI	ATGAA	2 cut(s)	67, 543
TspRI	CASTG	6 cut(s)	14, 262, 684, 1057, 1239, 1289
Vha464I	CTTAAG	1 cut(s)	776
VpaK11BI	GGWCC	2 cut(s)	406, 1129
XapI	RAATTY	5 cut(s)	111, 120, 267, 683, 1220
XceI	RCATGY	3 cut(s)	237, 403, 785
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1117
XmiI	GTMKAC	1 cut(s)	897
XmnI	GAANNNNTTC	1 cut(s)	1093
ZrmI	AGTACT	1 cut(s)	455
Zsp2I	ATGCAT	1 cut(s)	787

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.