Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006411

Physical Location & Seq

Genomic Coordinates Forward (+)

25660026 .. 25664468

Gene Structure 4443 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1165900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGAAAAGTGTCCGAACTCCTCACGAGTCTCCTGCTGGATGGACATCCTCGCCCCAGCCCCAAGGACCACTGTCACCAATACGGCCTGAGAGGCAAAACTATGATGATCCAATGGGATATCCTTTCGAAGATTTTCATAGTGGAGGTGGCTCCCAGTCTTCCATAGATAAGCAACGGCCAAGTTCTGGTGTGACAAAGGGCAAGAATGAGCCAGCAAGTGTACTCATGGAAGGACTGACAGCCAATCTTAAGGACATTGGCTTAGGGGAAATGATACTAACCCGATGGCAGTGCCTGGAAATTCTGGTACAATCCATCCCAAGCTCAGCATCAGGGCAGGGAATTCCTTCAGAAGGCAATTCAGGACGGTCCAGCAGGAAAAGGCCTCATTCTGCATCCGGGGGCAACATTAGACTTGAACCATTACTAGAAATGAGTCACCCTGATGTACATTTCAAGTTGTCGAGCTCACCTGAGCAAGTCCCGGCATTTGATCAAGCTCCAAGTCCATCGACGCGAGTTTTGTTCTGCGCTGATCATCCATATTTGGTCGGAACTGCAAGGGGAGATGCATTTTGGAAGAGGCTACAAGAATGTATGCATGCATGTGGTTTCGACACCATTAGCAACATTTTGGCCGACGTGGAGAATTTGTTAAAGTGGCTGCAGAATTGTATGCATGCTTCTGGTTTTGACATCATCAGCAACATTTTGGCTAATGCGGAGAATTTGGTCAAGATTTTTGATACCCCTGAAGGCTTGATTTCCAGTGGTAAAATGGGAACTGGGAAGTCTGCAAAGAAACCTGATAAAGGAAAAGCTGCAATGGCGGAGGCATGCATGCGTGAGAAGGTCCGAGAAATACAGAAAAACCTGTCCTCCATGCTTAGAGCTCTCGGGGAATTGGCAATTCCTAATCCTAATTTCAGACAGAGTCGGCTTTCTTCCCTGGCTTTGGAAACATCAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005488 GO:0006417 GO:0006446 GO:0006448 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0014070 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031667 GO:0031668 GO:0031669 GO:0032056 GO:0032058 GO:0032268 GO:0032270 GO:0033554 GO:0034198 GO:0034248 GO:0034250 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0043021 GO:0043022 GO:0043555 GO:0043558 GO:0044877 GO:0045727 GO:0045948 GO:0046677 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051716 GO:0060255 GO:0060992 GO:0065007 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071262 GO:0071264 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0080090 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_EZ

Protein Analysis

324

Amino Acids

35.04

Weight (kDa)

7.66

Isoelectric Point (pI)

68.17

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006411)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g00980 FvH4_6g00980 FvH4_6g00980
malus_domestica	MD04G1165900.v1.1 MD04G1239500.v1.1 MD12G1256600.v1.1
prunus_persica	Prupe.3G301900_v2.0.a1 Prupe.3G302000_v2.0.a1 Prupe.6G052100_v2.0.a1 Prupe.6G357400_v2.0.a1 Prupe.6G357700_v2.0.a1
pyrus_communis	pycom04g14760 pycom04g21150 pycom12g23530
rosa_chinensis	RchiOBHm_Chr3g0448441
rosa_laevigata	RLG00000025914
rosa_multiflora	Rmu_sc0003308.1_g000010 Rmu_sc0011979.1_g000002 Rmu_sc0024371.1_g000001 Rmu_sc0027902.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426630
rosa_rugosa	Rorug02G0612500
rosa_samantha	Rh3AG011900 Rh3BG011700 Rh3CG011400 Rh3DG012100
rosa_wichuraiana	Rw3G000920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	185
AccII	CGCG	1 cut(s)	517
AciI	CCGC	2 cut(s)	722, 830
AclWI	GGATC	1 cut(s)	101
AcoI	YGGCCR	2 cut(s)	176, 636
AcsI	RAATTY	4 cut(s)	300, 342, 649, 727
AcuI	CTGAAG	2 cut(s)	333, 774
AfaI	GTAC	3 cut(s)	222, 309, 450
AfiI	CCNNNNNNNGG	4 cut(s)	185, 353, 812, 955
AflII	CTTAAG	1 cut(s)	248
AgsI	TTSAA	2 cut(s)	419, 457
AjiI	CACGTC	1 cut(s)	643
AjnI	CCWGG	2 cut(s)	294, 948
AluBI	AGCT	5 cut(s)	324, 468, 500, 821, 893
AluI	AGCT	5 cut(s)	324, 468, 500, 821, 893
Alw21I	GWGCWC	2 cut(s)	470, 895
Alw26I	GTCTC	1 cut(s)	33
AlwI	GGATC	1 cut(s)	101
AlwNI	CAGNNNCTG	1 cut(s)	295
Ama87I	CYCGRG	1 cut(s)	896
AoxI	GGCC	4 cut(s)	83, 176, 383, 636
ApeKI	GCWGC	2 cut(s)	664, 821
ApoI	RAATTY	4 cut(s)	300, 342, 649, 727
ArsI	GACNNNNNNTTYG	2 cut(s)	505, 537
Asp700I	GAANNNNTTC	2 cut(s)	132, 346
AspLEI	GCGC	1 cut(s)	533
AspS9I	GGNCC	3 cut(s)	65, 369, 853
AsuC2I	CCSGG	2 cut(s)	400, 485
AsuHPI	GGTGA	3 cut(s)	66, 431, 462
AsuII	TTCGAA	1 cut(s)	126
AvaI	CYCGRG	1 cut(s)	896
AvaII	GGWCC	3 cut(s)	65, 369, 853
BanII	GRGCYC	2 cut(s)	470, 895
BarI	GAAGNNNNNNTAC	2 cut(s)	667, 699
BauI	CACGAG	1 cut(s)	23
BbsI	GAAGAC	1 cut(s)	150
Bbv12I	GWGCWC	2 cut(s)	470, 895
BbvI	GCAGC	2 cut(s)	651, 808
BccI	CCATC	4 cut(s)	33, 279, 323, 517
BceAI	ACGGC	2 cut(s)	98, 191
BcgI	CGANNNNNNTGC	4 cut(s)	14, 48, 273, 307
BciT130I	CCWGG	2 cut(s)	296, 950
BclI	TGATCA	2 cut(s)	493, 535
BcnI	CCSGG	2 cut(s)	400, 485
BcoDI	GTCTC	1 cut(s)	33
BfaI	CTAG	1 cut(s)	428
BfmI	CTRYAG	1 cut(s)	665
BfrI	CTTAAG	1 cut(s)	248
BglI	GCCNNNNNGGC	1 cut(s)	91
BisI	GCNGC	2 cut(s)	665, 822
BlpI	GCTNAGC	1 cut(s)	325
BlsI	GCNGC	2 cut(s)	666, 823
Bme1390I	CCNGG	4 cut(s)	296, 400, 485, 950
Bme18I	GGWCC	3 cut(s)	65, 369, 853
BmeT110I	CYCGRG	1 cut(s)	896
BmgBI	CACGTC	1 cut(s)	643
BmgT120I	GGNCC	3 cut(s)	65, 369, 853
BmiI	GGNNCC	1 cut(s)	151
BmrFI	CCNGG	4 cut(s)	296, 400, 485, 950
BmrI	ACTGGG	2 cut(s)	148, 795
BmsI	GCATC	3 cut(s)	338, 404, 559
BmuI	ACTGGG	2 cut(s)	148, 795
BoxI	GACNNNNGTC	1 cut(s)	70
BpiI	GAAGAC	1 cut(s)	150
Bpu10I	CCTNAGC	2 cut(s)	262, 474
Bpu1102I	GCTNAGC	1 cut(s)	325
Bpu14I	TTCGAA	1 cut(s)	126
BpuMI	CCSGG	2 cut(s)	400, 485
BsaBI	GATNNNNATC	1 cut(s)	43
BsaJI	CCNNGG	3 cut(s)	61, 399, 948
BsaXI	ACNNNNNCTCC	4 cut(s)	638, 668, 863, 893
Bsc4I	CCNNNNNNNGG	4 cut(s)	185, 353, 812, 955
Bse1I	ACTGG	3 cut(s)	154, 768, 790
Bse3DI	GCAATG	1 cut(s)	831
Bse8I	GATNNNNATC	1 cut(s)	43
BseBI	CCWGG	2 cut(s)	296, 950
BseDI	CCNNGG	3 cut(s)	61, 399, 948
BseGI	GGATG	5 cut(s)	44, 44, 315, 395, 538
BseJI	GATNNNNATC	1 cut(s)	43
BseLI	CCNNNNNNNGG	4 cut(s)	185, 353, 812, 955
BseMI	GCAATG	1 cut(s)	831
BseMII	CTCAG	3 cut(s)	78, 339, 465
BseNI	ACTGG	3 cut(s)	154, 768, 790
BseRI	GAGGAG	1 cut(s)	9
BseXI	GCAGC	2 cut(s)	651, 808
BseYI	CCCAGC	1 cut(s)	54
Bsh1236I	CGCG	1 cut(s)	517
BshFI	GGCC	4 cut(s)	85, 178, 385, 638
BsiHKAI	GWGCWC	2 cut(s)	470, 895
BsiHKCI	CYCGRG	1 cut(s)	896
BsiSI	CCGG	2 cut(s)	399, 485
BslFI	GGGAC	1 cut(s)	467
BslI	CCNNNNNNNGG	4 cut(s)	185, 353, 812, 955
BsmAI	GTCTC	1 cut(s)	33
BsmFI	GGGAC	1 cut(s)	467
BsnI	GGCC	4 cut(s)	85, 178, 385, 638
BsoBI	CYCGRG	1 cut(s)	896
Bsp119I	TTCGAA	1 cut(s)	126
Bsp1286I	GDGCHC	2 cut(s)	470, 895
Bsp1407I	TGTACA	1 cut(s)	448
Bsp143I	GATC	3 cut(s)	106, 493, 535
Bsp1720I	GCTNAGC	1 cut(s)	325
BspACI	CCGC	2 cut(s)	722, 830
BspANI	GGCC	4 cut(s)	85, 178, 385, 638
BspCNI	CTCAG	3 cut(s)	79, 338, 466
BspFNI	CGCG	1 cut(s)	517
BspLI	GGNNCC	1 cut(s)	151
BspMAI	CTGCAG	1 cut(s)	669
BspPI	GGATC	1 cut(s)	101
BspT104I	TTCGAA	1 cut(s)	126
BspTI	CTTAAG	1 cut(s)	248
BsrDI	GCAATG	1 cut(s)	831
BsrGI	TGTACA	1 cut(s)	448
BsrI	ACTGG	3 cut(s)	154, 768, 790
BssECI	CCNNGG	3 cut(s)	61, 399, 948
BssMI	GATC	3 cut(s)	106, 493, 535
BssSI	CACGAG	1 cut(s)	23
BssT1I	CCWWGG	1 cut(s)	61
Bst2BI	CACGAG	1 cut(s)	23
Bst2UI	CCWGG	2 cut(s)	296, 950
Bst4CI	ACNGT	2 cut(s)	72, 369
Bst6I	CTCTTC	1 cut(s)	575
BstAFI	CTTAAG	1 cut(s)	248
BstAUI	TGTACA	1 cut(s)	448
BstBI	TTCGAA	1 cut(s)	126
BstC8I	GCNNGC	5 cut(s)	213, 603, 681, 838, 842
BstDEI	CTNAG	5 cut(s)	87, 262, 325, 474, 887
BstENI	CCTNNNNNAGG	2 cut(s)	351, 810
BstF5I	GGATG	5 cut(s)	44, 44, 315, 395, 538
BstFNI	CGCG	1 cut(s)	517
BstHHI	GCGC	1 cut(s)	533
BstKTI	GATC	3 cut(s)	109, 496, 538
BstMAI	GTCTC	1 cut(s)	33
BstMBI	GATC	3 cut(s)	106, 493, 535
BstMWI	GCNNNNNNNGC	2 cut(s)	91, 827
BstNI	CCWGG	2 cut(s)	296, 950
BstNSI	RCATGY	5 cut(s)	605, 609, 683, 840, 844
BstPAI	GACNNNNGTC	1 cut(s)	70
BstSCI	CCNGG	4 cut(s)	294, 398, 483, 948
BstSFI	CTRYAG	1 cut(s)	665
BstUI	CGCG	1 cut(s)	517
BstV1I	GCAGC	2 cut(s)	651, 808
BstV2I	GAAGAC	1 cut(s)	150
BsuRI	GGCC	4 cut(s)	85, 178, 385, 638
BtrI	CACGTC	1 cut(s)	643
BtsCI	GGATG	5 cut(s)	44, 44, 315, 395, 538
BtsI	GCAGTG	1 cut(s)	296
BtsIMutI	CAGTG	3 cut(s)	68, 296, 775
Cac8I	GCNNGC	5 cut(s)	213, 603, 681, 838, 842
CaiI	CAGNNNCTG	1 cut(s)	295
CfoI	GCGC	1 cut(s)	533
Cfr13I	GGNCC	3 cut(s)	65, 369, 853
CseI	GACGC	1 cut(s)	523
Csp6I	GTAC	3 cut(s)	221, 308, 449
CviAII	CATG	7 cut(s)	226, 602, 606, 680, 837, 841, 883
CviQI	GTAC	3 cut(s)	221, 308, 449
DdeI	CTNAG	5 cut(s)	87, 262, 325, 474, 887
DpnI	GATC	3 cut(s)	108, 495, 537
DpnII	GATC	3 cut(s)	106, 493, 535
EaeI	YGGCCR	2 cut(s)	176, 636
Eam1104I	CTCTTC	1 cut(s)	575
EarI	CTCTTC	1 cut(s)	575
EciI	GGCGGA	1 cut(s)	845
Ecl136II	GAGCTC	2 cut(s)	468, 893
Eco130I	CCWWGG	1 cut(s)	61
Eco147I	AGGCCT	1 cut(s)	385
Eco24I	GRGCYC	2 cut(s)	470, 895
Eco32I	GATATC	1 cut(s)	119
Eco47I	GGWCC	3 cut(s)	65, 369, 853
Eco53kI	GAGCTC	2 cut(s)	468, 893
Eco57I	CTGAAG	2 cut(s)	333, 774
Eco88I	CYCGRG	1 cut(s)	896
EcoICRI	GAGCTC	2 cut(s)	468, 893
EcoNI	CCTNNNNNAGG	2 cut(s)	351, 810
EcoRI	GAATTC	1 cut(s)	342
EcoRII	CCWGG	2 cut(s)	294, 948
EcoRV	GATATC	1 cut(s)	119
EcoT14I	CCWWGG	1 cut(s)	61
EcoT22I	ATGCAT	5 cut(s)	574, 603, 607, 681, 842
EcoT38I	GRGCYC	2 cut(s)	470, 895
ErhI	CCWWGG	1 cut(s)	61
FaeI	CATG	7 cut(s)	229, 605, 609, 683, 840, 844, 886
FaqI	GGGAC	1 cut(s)	467
FatI	CATG	7 cut(s)	225, 601, 605, 679, 836, 840, 882
FbaI	TGATCA	2 cut(s)	493, 535
Fnu4HI	GCNGC	2 cut(s)	665, 822
FokI	GGATG	5 cut(s)	31, 51, 302, 382, 525
FriOI	GRGCYC	2 cut(s)	470, 895
Fsp4HI	GCNGC	2 cut(s)	665, 822
FspBI	CTAG	1 cut(s)	428
GlaI	GCGC	1 cut(s)	532
GluI	GCNGC	2 cut(s)	665, 822
GsaI	CCCAGC	1 cut(s)	58
HaeIII	GGCC	4 cut(s)	85, 178, 385, 638
HapII	CCGG	2 cut(s)	399, 485
HgaI	GACGC	1 cut(s)	523
HhaI	GCGC	1 cut(s)	533
Hin1II	CATG	7 cut(s)	229, 605, 609, 683, 840, 844, 886
Hin6I	GCGC	1 cut(s)	531
HinP1I	GCGC	1 cut(s)	531
HinfI	GANTC	3 cut(s)	26, 436, 934
HpaII	CCGG	2 cut(s)	399, 485
HphI	GGTGA	3 cut(s)	66, 431, 462
Hpy166II	GTNNAC	1 cut(s)	221
Hpy188I	TCNGA	5 cut(s)	14, 352, 554, 857, 929
Hpy188III	TCNNGA	3 cut(s)	23, 363, 736
Hpy8I	GTNNAC	1 cut(s)	221
Hpy99I	CGWCG	2 cut(s)	517, 644
HpyAV	CCTTC	5 cut(s)	224, 347, 357, 749, 844
HpyCH4III	ACNGT	2 cut(s)	72, 369
HpyCH4IV	ACGT	1 cut(s)	642
HpyF10VI	GCNNNNNNNGC	2 cut(s)	91, 827
HpyF3I	CTNAG	5 cut(s)	87, 262, 325, 474, 887
HpySE526I	ACGT	1 cut(s)	642
Hsp92II	CATG	7 cut(s)	229, 605, 609, 683, 840, 844, 886
HspAI	GCGC	1 cut(s)	531
Ksp22I	TGATCA	2 cut(s)	493, 535
Kzo9I	GATC	3 cut(s)	106, 493, 535
LmnI	GCTCC	2 cut(s)	155, 505
Lsp1109I	GCAGC	2 cut(s)	651, 808
LweI	GCATC	3 cut(s)	338, 404, 559
MaeI	CTAG	1 cut(s)	428
MaeII	ACGT	1 cut(s)	642
MaeIII	GTNAC	3 cut(s)	72, 190, 437
MalI	GATC	3 cut(s)	108, 495, 537
MboI	GATC	3 cut(s)	106, 493, 535
MboII	GAAGA	4 cut(s)	140, 150, 592, 936
MhlI	GDGCHC	2 cut(s)	470, 895
MlyI	GAGTC	3 cut(s)	35, 445, 943
MmeI	TCCRAC	1 cut(s)	532
MnlI	CCTC	8 cut(s)	30, 58, 84, 137, 396, 576, 826, 889
Mph1103I	ATGCAT	5 cut(s)	574, 603, 607, 681, 842
MroXI	GAANNNNTTC	2 cut(s)	132, 346
MseI	TTAA	2 cut(s)	249, 656
MslI	CAYNNNNRTG	2 cut(s)	444, 606
MspCI	CTTAAG	1 cut(s)	248
MspI	CCGG	2 cut(s)	399, 485
MspR9I	CCNGG	4 cut(s)	296, 400, 485, 950
MvaI	CCWGG	2 cut(s)	296, 950
MvnI	CGCG	1 cut(s)	517
MwoI	GCNNNNNNNGC	2 cut(s)	91, 827
NciI	CCSGG	2 cut(s)	400, 485
NdeII	GATC	3 cut(s)	106, 493, 535
NlaIII	CATG	7 cut(s)	229, 605, 609, 683, 840, 844, 886
NlaIV	GGNNCC	1 cut(s)	151
NmuCI	GTSAC	3 cut(s)	72, 190, 437
NsiI	ATGCAT	5 cut(s)	574, 603, 607, 681, 842
NspI	RCATGY	5 cut(s)	605, 609, 683, 840, 844
NspV	TTCGAA	1 cut(s)	126
PaeI	GCATGC	4 cut(s)	605, 683, 840, 844
PceI	AGGCCT	1 cut(s)	385
PdmI	GAANNNNTTC	2 cut(s)	132, 346
PflFI	GACNNNGTC	1 cut(s)	933
PflMI	CCANNNNNTGG	1 cut(s)	185
PkrI	GCNGC	2 cut(s)	666, 823
PleI	GAGTC	3 cut(s)	34, 444, 942
PpsI	GAGTC	3 cut(s)	34, 444, 942
PshAI	GACNNNNGTC	1 cut(s)	70
Psp124BI	GAGCTC	2 cut(s)	470, 895
Psp6I	CCWGG	2 cut(s)	294, 948
PspFI	CCCAGC	1 cut(s)	54
PspGI	CCWGG	2 cut(s)	294, 948
PspN4I	GGNNCC	1 cut(s)	151
PspPI	GGNCC	3 cut(s)	65, 369, 853
PstI	CTGCAG	1 cut(s)	669
PstNI	CAGNNNCTG	1 cut(s)	295
PsyI	GACNNNGTC	1 cut(s)	933
RsaI	GTAC	3 cut(s)	222, 309, 450
RsaNI	GTAC	3 cut(s)	221, 308, 449
RseI	CAYNNNNRTG	2 cut(s)	444, 606
SacI	GAGCTC	2 cut(s)	470, 895
SaqAI	TTAA	2 cut(s)	249, 656
SatI	GCNGC	2 cut(s)	665, 822
Sau3AI	GATC	3 cut(s)	106, 493, 535
Sau96I	GGNCC	3 cut(s)	65, 369, 853
SchI	GAGTC	3 cut(s)	35, 445, 943
ScrFI	CCNGG	4 cut(s)	296, 400, 485, 950
SduI	GDGCHC	2 cut(s)	470, 895
SfaNI	GCATC	3 cut(s)	338, 404, 559
SfcI	CTRYAG	1 cut(s)	665
SfuI	TTCGAA	1 cut(s)	126
SinI	GGWCC	3 cut(s)	65, 369, 853
SmiMI	CAYNNNNRTG	2 cut(s)	444, 606
SmlI	CTYRAG	1 cut(s)	248
SmoI	CTYRAG	1 cut(s)	248
SphI	GCATGC	4 cut(s)	605, 683, 840, 844
SseBI	AGGCCT	1 cut(s)	385
SsiI	CCGC	2 cut(s)	722, 830
SspMI	CTAG	1 cut(s)	428
SstI	GAGCTC	2 cut(s)	470, 895
StuI	AGGCCT	1 cut(s)	385
StyD4I	CCNGG	4 cut(s)	294, 398, 483, 948
StyI	CCWWGG	1 cut(s)	61
TaaI	ACNGT	2 cut(s)	72, 369
TaiI	ACGT	1 cut(s)	645
TaqI	TCGA	4 cut(s)	126, 464, 512, 615
TatI	WGTACW	2 cut(s)	220, 448
Tru1I	TTAA	2 cut(s)	249, 656
Tru9I	TTAA	2 cut(s)	249, 656
TscAI	CASTG	3 cut(s)	75, 296, 775
TseFI	GTSAC	3 cut(s)	72, 190, 437
TseI	GCWGC	2 cut(s)	664, 821
Tsp45I	GTSAC	3 cut(s)	72, 190, 437
TspDTI	ATGAA	2 cut(s)	17, 125
TspRI	CASTG	3 cut(s)	75, 296, 775
Tth111I	GACNNNGTC	1 cut(s)	933
Van91I	CCANNNNNTGG	1 cut(s)	185
Vha464I	CTTAAG	1 cut(s)	248
VpaK11BI	GGWCC	3 cut(s)	65, 369, 853
XagI	CCTNNNNNAGG	2 cut(s)	351, 810
XapI	RAATTY	4 cut(s)	300, 342, 649, 727
XceI	RCATGY	5 cut(s)	605, 609, 683, 840, 844
XcmI	CCANNNNNNNNNTGG	1 cut(s)	775
XmnI	GAANNNNTTC	2 cut(s)	132, 346
XspI	CTAG	1 cut(s)	428
Zsp2I	ATGCAT	5 cut(s)	574, 603, 607, 681, 842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.