Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006411

Physical Location & Seq

Genomic Coordinates Reverse (-)

32418875 .. 32424438

Gene Structure 5564 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1256600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2415 bp

ATGTCAGGGACCAAAGTGCTTTTAACATACAAGCGAAAGCGGCGATCAAGAAAAGCTCTTGAAGAGGGACATGATTGCCATAATTCACTTTTTGTGGCTCCGAGTGACACTTCTCTAAGTAGAGAAGACTTGCAACTTCATTTGATTGATAAGTGTGCACCAGAAGACTATGACAGAAATGATTCTTCAATACCTCATTCAATACGAAAATCAAGCAGACTGGACAGTCTCGATGAGGGTCTCTCCCGTCACAAGAAAGTTGGCTCGTCCTCAAATACTAATAATGAAGCATTGGTTGACGATGATAATGCTGGAGGAAAGTTAGTTTCCGGGTTAATGGGGAATGCCACTGAAATGACCGCTGATTTTGTCTGGCCGAAATCATCATCATCATCATCATCATCATCATTTGAAAGAAAAGGCACCTCTGAATGTGATGGTATTTTGCTAAGATTGAATGCATCAAACTTGGAAGAGACTGATCCGTTCTCTAAAATTAATGCAGATAACTTATGCTGCGATTCAGCTGTGCAGACCAAGTCGACTGCCCCATTAATTACTTTCTGCCGAAGGAAAACAAGAAGAAAGGATATAGATAAGTCTGATAAACAAAGCATATCTGTGCCTGCAGAAAATGATTGCTCATTGGTAACCAAATTGAGTGATTGTGTTTGTGCTAATGCCGCTTCGTGCGAAGAAACTTCCCCTGAAAAGTGCTCCATCGATCATGAGACAGATTTGAAGCACTCCAGAAACAAAGCTCAAGTTTTGTCAAGTGATGATCTTAAAGCGACGGAACTCCCTTGTGTAGGCCAATCACTTCCTTATTTGCACCTGTCAGTTATCCCAACCGATTCATGTGGCACCGACTGCAATTTTGACTTGAACTTGAGTCCTCAACAGCAACCTAATATTGCTGCGCCAAAAACTATGGGGGGCTCCTTGGATTCTACTAGCCGAAATCATGCCGCTGTATTACATGAACTGTCTCCTCCACAAATGCTGGCTGTGAGAAATGAAAGAGTAGAAACTCAAGCTTCACAATTACACAGAGATGCATTTGAATTTTTAGAAGTTGGTGCCAGCTGCAAAGATAATGATGATAAAGTTGGTTATCTGATTTCCGAGGAATTTACTTCTGAAAACAAATGTCTGCAGCTGTTTCCAGAAGAAAAAACCAGTTACATTTTTTGCCCGGTGATAACACAACCTGAGGTAGCTGCTTCTATAGCTTCAGAACGGAGAAAAGTTCTTCTGTTGGGAGGCCAGAATAATCAACCAAAACAAGGATCTCCTATGTTTTTGGGTCTATCCTTAACCGAAAAGCCTGTACTTGCAGGATGTGCTGCCAATACCTGCTTCAATACATCTCCCTTCTTGAACTCCATCATTGGAACAAGAGAATTCATCCGAGATGCAGCGCTCCAATCTTCCTCAAGCCGTTTATCATCAGTTTTGAAACACAAGATTATGCATGACAGTGTTGTAAGCCAAGCAAGAGCTTTGAATGAGAGGGGTAGTTTTCATGACAATTATATGCCATATAATACCATGTGGTCTGAAGAAGAGTTGGATTTTCTATGGATGGGCGTGAGGAGATATGGACGGGACAATTGGAATGCTATGTTAAGGGATCCAAGTTTTCACTTCTCACCATGGAGGGTGGCAAGGGACTTAGCTGAGCGGTGGGAAGAGGAACAGTCAAAACTTTTTAGTGGTTTATGTGTTCCTCAATTGGGATGCAACTACTTCTCGGGTCCAAAAAGAGGAATATGGAAAGAAAATACAACAGACATAACCCAAGTTTCACTTGGCGATGTTGATGCTCATAGAGGAGGTAATGCCTCGAGGAGGCCACTATTTAAGTCGGCTTATATTTGCAACAATGGCAATGGAAATCCCCGGAGGCCTCTTGGTTACACAAAAAGGACATCTCGTTTTGAAATGGGAAGGGACAAATATGAGGATGATGAATTTAGTGTTCTAAATAGAAGTAGAACTATTCGAAGGGGCAAGTTATTATCAACTGATGGCCCCACAACTTGTGTTGGAGCAAAGGGAAATTTACCCCACTGGCTCAGAGAAGCTGTTGCTTCTCCACCGCCAACTGTGCCTTCAACTGTTTCATCAATTGCTCATTCAGATAGGTTGAATCTCACCCTTCTCAGATTCGATCCTCGGGGATCACATTTGGCACCAAGGAATGAAATGCAGTTTACATGTGGTGGTTTAAGAGTAAATGACTCACAACCATTAAGTACTGCTCCTCTTTTCAACTACTCATCTGTAGGACTAGGGATGGGGAAACTGAGAAGGGATTATTCTCATCACGCGGGTAAACAAGATGTGATTATTATAGACAGCGATGGTTCTTCTGAAGAGACTATATCTGATGATTGTAGTGCAAGGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

805

Amino Acids

88.95

Weight (kDa)

6.42

Isoelectric Point (pI)

56.14

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006411)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g00980 FvH4_6g00980 FvH4_6g00980
malus_domestica	MD04G1165900.v1.1 MD04G1239500.v1.1 MD12G1256600.v1.1
prunus_persica	Prupe.3G301900_v2.0.a1 Prupe.3G302000_v2.0.a1 Prupe.6G052100_v2.0.a1 Prupe.6G357400_v2.0.a1 Prupe.6G357700_v2.0.a1
pyrus_communis	pycom04g14760 pycom04g21150 pycom12g23530
rosa_chinensis	RchiOBHm_Chr3g0448441
rosa_laevigata	RLG00000025914
rosa_multiflora	Rmu_sc0003308.1_g000010 Rmu_sc0011979.1_g000002 Rmu_sc0024371.1_g000001 Rmu_sc0027902.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426630
rosa_rugosa	Rorug02G0612500
rosa_samantha	Rh3AG011900 Rh3BG011700 Rh3CG011400 Rh3DG012100
rosa_wichuraiana	Rw3G000920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	225
AbsI	CCTCGAGG	1 cut(s)	1846
Acc36I	ACCTGC	1 cut(s)	1364
AccB1I	GGYRCC	4 cut(s)	422, 863, 1079, 2194
AccBSI	CCGCTC	1 cut(s)	1684
AccI	GTMKAC	1 cut(s)	542
AccII	CGCG	1 cut(s)	2333
AciI	CCGC	7 cut(s)	40, 360, 684, 969, 1684, 2102, 2333
AclWI	GGATC	6 cut(s)	476, 1297, 1628, 1641, 2168, 2191
AcoI	YGGCCR	1 cut(s)	374
AcsI	RAATTY	5 cut(s)	1064, 1130, 1403, 1973, 2062
AcuI	CTGAAG	3 cut(s)	1218, 1581, 2397
AfaI	GTAC	2 cut(s)	1332, 2260
AfeI	AGCGCT	1 cut(s)	1422
AfiI	CCNNNNNNNGG	5 cut(s)	809, 1286, 1736, 1766, 1851
AflIII	ACRYGT	1 cut(s)	2219
AjuI	GAANNNNNNNTTGG	4 cut(s)	1343, 1375, 2098, 2130
Alw21I	GWGCWC	2 cut(s)	160, 719
Alw26I	GTCTC	6 cut(s)	233, 245, 470, 725, 993, 2375
Alw44I	GTGCAC	1 cut(s)	156
AlwI	GGATC	6 cut(s)	476, 1297, 1628, 1641, 2168, 2191
Ama87I	CYCGRG	3 cut(s)	1753, 1846, 2178
Aor51HI	AGCGCT	1 cut(s)	1422
AoxI	GGCC	6 cut(s)	374, 811, 1264, 1853, 1907, 2032
ApaLI	GTGCAC	1 cut(s)	156
ApeKI	GCWGC	7 cut(s)	516, 917, 1086, 1156, 1220, 1346, 1418
ApoI	RAATTY	5 cut(s)	1064, 1130, 1403, 1973, 2062
ArsI	GACNNNNNNTTYG	2 cut(s)	860, 892
AseI	ATTAAT	2 cut(s)	498, 554
Asp700I	GAANNNNTTC	1 cut(s)	181
AspLEI	GCGC	2 cut(s)	922, 1423
AspS9I	GGNCC	3 cut(s)	9, 1757, 2033
AsuC2I	CCSGG	3 cut(s)	331, 1196, 1903
AsuHPI	GGTGA	3 cut(s)	1210, 1644, 2149
AsuII	TTCGAA	1 cut(s)	2005
AvaI	CYCGRG	3 cut(s)	1753, 1846, 2178
AvaII	GGWCC	2 cut(s)	9, 1757
AxyI	CCTNAGG	1 cut(s)	1212
BaeGI	GKGCMC	1 cut(s)	160
BamHI	GGATCC	1 cut(s)	1633
BanI	GGYRCC	4 cut(s)	422, 863, 1079, 2194
BanII	GRGCYC	1 cut(s)	941
BbsI	GAAGAC	2 cut(s)	132, 171
Bbv12I	GWGCWC	2 cut(s)	160, 719
BbvI	GCAGC	7 cut(s)	503, 904, 1073, 1168, 1207, 1333, 1430
BccI	CCATC	7 cut(s)	431, 728, 1394, 1579, 2024, 2293, 2360
BceAI	ACGGC	1 cut(s)	1425
BcgI	CGANNNNNNTGC	4 cut(s)	290, 324, 1805, 1839
BcnI	CCSGG	3 cut(s)	331, 1196, 1903
BcoDI	GTCTC	6 cut(s)	233, 245, 470, 725, 993, 2375
BfaI	CTAG	2 cut(s)	954, 2294
BfmI	CTRYAG	5 cut(s)	627, 1154, 1227, 2286, 2411
BfoI	RGCGCY	1 cut(s)	1424
BfuAI	ACCTGC	1 cut(s)	1364
BlpI	GCTNAGC	1 cut(s)	1680
BmcAI	AGTACT	1 cut(s)	2260
Bme1390I	CCNGG	3 cut(s)	331, 1196, 1903
Bme18I	GGWCC	2 cut(s)	9, 1757
BmeT110I	CYCGRG	3 cut(s)	1753, 1846, 2178
BmgT120I	GGNCC	3 cut(s)	9, 1757, 2033
BmrFI	CCNGG	3 cut(s)	331, 1196, 1903
BmsI	GCATC	5 cut(s)	470, 1045, 1405, 1730, 1813
BpiI	GAAGAC	2 cut(s)	132, 171
BplI	GAGNNNNNCTC	2 cut(s)	227, 259
BpmI	CTGGAG	2 cut(s)	333, 733
Bpu1102I	GCTNAGC	1 cut(s)	1680
Bpu14I	TTCGAA	1 cut(s)	2005
BpuEI	CTTGAG	4 cut(s)	747, 910, 1017, 1420
BpuMI	CCSGG	3 cut(s)	331, 1196, 1903
Bsa29I	ATCGAT	1 cut(s)	723
BsaI	GGTCTC	1 cut(s)	245
BsaJI	CCNNGG	6 cut(s)	942, 1125, 1655, 1901, 2177, 2198
Bsc4I	CCNNNNNNNGG	5 cut(s)	809, 1286, 1736, 1766, 1851
Bse1I	ACTGG	3 cut(s)	225, 1179, 2078
Bse21I	CCTNAGG	1 cut(s)	1212
Bse3DI	GCAATG	1 cut(s)	1897
BseCI	ATCGAT	1 cut(s)	723
BseDI	CCNNGG	6 cut(s)	942, 1125, 1655, 1901, 2177, 2198
BseGI	GGATG	6 cut(s)	1346, 1407, 1590, 1745, 1972, 2304
BseLI	CCNNNNNNNGG	5 cut(s)	809, 1286, 1736, 1766, 1851
BseMI	GCAATG	1 cut(s)	1897
BseMII	CTCAG	5 cut(s)	1203, 1671, 2092, 2179, 2300
BseNI	ACTGG	3 cut(s)	225, 1179, 2078
BseRI	GAGGAG	5 cut(s)	981, 1609, 1848, 1864, 2256
BseSI	GKGCMC	1 cut(s)	160
BseXI	GCAGC	7 cut(s)	503, 904, 1073, 1168, 1207, 1333, 1430
BsgI	GTGCAG	1 cut(s)	551
Bsh1236I	CGCG	1 cut(s)	2333
BshFI	GGCC	6 cut(s)	376, 813, 1266, 1855, 1909, 2034
BshNI	GGYRCC	4 cut(s)	422, 863, 1079, 2194
BshVI	ATCGAT	1 cut(s)	723
BsiHKAI	GWGCWC	2 cut(s)	160, 719
BsiHKCI	CYCGRG	3 cut(s)	1753, 1846, 2178
BsiSI	CCGG	3 cut(s)	330, 1196, 1903
BslFI	GGGAC	5 cut(s)	22, 81, 1622, 1685, 1967
BslI	CCNNNNNNNGG	5 cut(s)	809, 1286, 1736, 1766, 1851
BsmAI	GTCTC	6 cut(s)	233, 245, 470, 725, 993, 2375
BsmFI	GGGAC	5 cut(s)	22, 81, 1622, 1685, 1967
BsmI	GAATGC	3 cut(s)	349, 463, 1624
BsnI	GGCC	6 cut(s)	376, 813, 1266, 1855, 1909, 2034
Bso31I	GGTCTC	1 cut(s)	245
BsoBI	CYCGRG	3 cut(s)	1753, 1846, 2178
Bsp119I	TTCGAA	1 cut(s)	2005
Bsp1286I	GDGCHC	3 cut(s)	160, 719, 941
Bsp143I	GATC	8 cut(s)	44, 481, 724, 781, 1289, 1633, 2173, 2183
Bsp1720I	GCTNAGC	1 cut(s)	1680
Bsp19I	CCATGG	1 cut(s)	1655
BspACI	CCGC	7 cut(s)	40, 360, 684, 969, 1684, 2102, 2333
BspANI	GGCC	6 cut(s)	376, 813, 1266, 1855, 1909, 2034
BspCNI	CTCAG	5 cut(s)	1204, 1672, 2091, 2178, 2301
BspDI	ATCGAT	1 cut(s)	723
BspFNI	CGCG	1 cut(s)	2333
BspHI	TCATGA	2 cut(s)	727, 1525
BspMAI	CTGCAG	2 cut(s)	631, 1158
BspMI	ACCTGC	1 cut(s)	1364
BspPI	GGATC	6 cut(s)	476, 1297, 1628, 1641, 2168, 2191
BspT104I	TTCGAA	1 cut(s)	2005
BspT107I	GGYRCC	4 cut(s)	422, 863, 1079, 2194
BspTNI	GGTCTC	1 cut(s)	245
BsrBI	CCGCTC	1 cut(s)	1684
BsrDI	GCAATG	1 cut(s)	1897
BsrI	ACTGG	3 cut(s)	225, 1179, 2078
BssECI	CCNNGG	6 cut(s)	942, 1125, 1655, 1901, 2177, 2198
BssMI	GATC	8 cut(s)	44, 481, 724, 781, 1289, 1633, 2173, 2183
BssT1I	CCWWGG	3 cut(s)	942, 1655, 2198
Bst4CI	ACNGT	6 cut(s)	227, 987, 1481, 1701, 2110, 2122
Bst6I	CTCTTC	5 cut(s)	57, 468, 1560, 1686, 2373
BstAPI	GCANNNNNTGC	2 cut(s)	870, 1343
BstBI	TTCGAA	1 cut(s)	2005
BstC8I	GCNNGC	3 cut(s)	627, 1005, 1084
BstDEI	CTNAG	8 cut(s)	116, 449, 1212, 1675, 1680, 2078, 2165, 2309
BstDSI	CCRYGG	1 cut(s)	1655
BstEII	GGTNACC	1 cut(s)	649
BstENI	CCTNNNNNAGG	2 cut(s)	807, 1849
BstF5I	GGATG	6 cut(s)	1346, 1407, 1590, 1745, 1972, 2304
BstFNI	CGCG	1 cut(s)	2333
BstH2I	RGCGCY	1 cut(s)	1424
BstHHI	GCGC	2 cut(s)	922, 1423
BstKTI	GATC	8 cut(s)	47, 484, 727, 784, 1292, 1636, 2176, 2186
BstMAI	GTCTC	6 cut(s)	233, 245, 470, 725, 993, 2375
BstMBI	GATC	8 cut(s)	44, 481, 724, 781, 1289, 1633, 2173, 2183
BstMWI	GCNNNNNNNGC	7 cut(s)	40, 683, 870, 1229, 1343, 1887, 2110
BstNSI	RCATGY	1 cut(s)	2223
BstPI	GGTNACC	1 cut(s)	649
BstSCI	CCNGG	3 cut(s)	329, 1194, 1901
BstSFI	CTRYAG	5 cut(s)	627, 1154, 1227, 2286, 2411
BstSLI	GKGCMC	1 cut(s)	160
BstUI	CGCG	1 cut(s)	2333
BstV1I	GCAGC	7 cut(s)	503, 904, 1073, 1168, 1207, 1333, 1430
BstV2I	GAAGAC	2 cut(s)	132, 171
BstX2I	RGATCY	2 cut(s)	1289, 1633
BstYI	RGATCY	2 cut(s)	1289, 1633
Bsu15I	ATCGAT	1 cut(s)	723
Bsu36I	CCTNAGG	1 cut(s)	1212
BsuRI	GGCC	6 cut(s)	376, 813, 1266, 1855, 1909, 2034
BsuTUI	ATCGAT	1 cut(s)	723
BtgI	CCRYGG	1 cut(s)	1655
BtgZI	GCGATG	2 cut(s)	1830, 2379
BtsCI	GGATG	6 cut(s)	1346, 1407, 1590, 1745, 1972, 2304
BtsIMutI	CAGTG	3 cut(s)	348, 1486, 2071
BveI	ACCTGC	1 cut(s)	1364
Cac8I	GCNNGC	3 cut(s)	627, 1005, 1084
CciI	TCATGA	2 cut(s)	727, 1525
CfoI	GCGC	2 cut(s)	922, 1423
Cfr13I	GGNCC	3 cut(s)	9, 1757, 2033
ClaI	ATCGAT	1 cut(s)	723
Csp6I	GTAC	2 cut(s)	1331, 2259
CviQI	GTAC	2 cut(s)	1331, 2259
DdeI	CTNAG	8 cut(s)	116, 449, 1212, 1675, 1680, 2078, 2165, 2309
DpnI	GATC	8 cut(s)	46, 483, 726, 783, 1291, 1635, 2175, 2185
DpnII	GATC	8 cut(s)	44, 481, 724, 781, 1289, 1633, 2173, 2183
DrdI	GACNNNNNNGTC	1 cut(s)	225
DseDI	GACNNNNNNGTC	1 cut(s)	225
EaeI	YGGCCR	1 cut(s)	374
Eam1104I	CTCTTC	5 cut(s)	57, 468, 1560, 1686, 2373
EarI	CTCTTC	5 cut(s)	57, 468, 1560, 1686, 2373
Eco130I	CCWWGG	3 cut(s)	942, 1655, 2198
Eco147I	AGGCCT	1 cut(s)	1909
Eco24I	GRGCYC	1 cut(s)	941
Eco31I	GGTCTC	1 cut(s)	245
Eco47I	GGWCC	2 cut(s)	9, 1757
Eco47III	AGCGCT	1 cut(s)	1422
Eco57I	CTGAAG	3 cut(s)	1218, 1581, 2397
Eco81I	CCTNAGG	1 cut(s)	1212
Eco88I	CYCGRG	3 cut(s)	1753, 1846, 2178
Eco91I	GGTNACC	1 cut(s)	649
EcoNI	CCTNNNNNAGG	2 cut(s)	807, 1849
EcoO65I	GGTNACC	1 cut(s)	649
EcoRI	GAATTC	1 cut(s)	1403
EcoT14I	CCWWGG	3 cut(s)	942, 1655, 2198
EcoT22I	ATGCAT	3 cut(s)	463, 1060, 1476
EcoT38I	GRGCYC	1 cut(s)	941
ErhI	CCWWGG	3 cut(s)	942, 1655, 2198
FalI	AAGNNNNNCTT	2 cut(s)	1794, 1826
FaqI	GGGAC	5 cut(s)	22, 81, 1622, 1685, 1967
FauI	CCCGC	1 cut(s)	2326
FblI	GTMKAC	1 cut(s)	542
FokI	GGATG	6 cut(s)	1353, 1394, 1597, 1752, 1979, 2311
FriOI	GRGCYC	1 cut(s)	941
FspBI	CTAG	2 cut(s)	954, 2294
GlaI	GCGC	2 cut(s)	921, 1422
GsuI	CTGGAG	2 cut(s)	333, 733
HaeII	RGCGCY	1 cut(s)	1424
HaeIII	GGCC	6 cut(s)	376, 813, 1266, 1855, 1909, 2034
HapII	CCGG	3 cut(s)	330, 1196, 1903
HhaI	GCGC	2 cut(s)	922, 1423
Hin6I	GCGC	2 cut(s)	920, 1421
HinP1I	GCGC	2 cut(s)	920, 1421
HincII	GTYRAC	2 cut(s)	298, 543
HindII	GTYRAC	2 cut(s)	298, 543
HindIII	AAGCTT	1 cut(s)	1035
HinfI	GANTC	8 cut(s)	182, 521, 854, 892, 947, 2152, 2169, 2243
HpaII	CCGG	3 cut(s)	330, 1196, 1903
HphI	GGTGA	3 cut(s)	1210, 1644, 2149
Hpy166II	GTNNAC	5 cut(s)	158, 298, 543, 2217, 2339
Hpy188III	TCNNGA	8 cut(s)	48, 59, 230, 728, 750, 1166, 1378, 1526
Hpy8I	GTNNAC	5 cut(s)	158, 298, 543, 2217, 2339
Hpy99I	CGWCG	1 cut(s)	796
HpyAV	CCTTC	7 cut(s)	564, 1384, 1944, 2001, 2124, 2171, 2307
HpyCH4III	ACNGT	6 cut(s)	227, 987, 1481, 1701, 2110, 2122
HpyF10VI	GCNNNNNNNGC	7 cut(s)	40, 683, 870, 1229, 1343, 1887, 2110
HpyF3I	CTNAG	8 cut(s)	116, 449, 1212, 1675, 1680, 2078, 2165, 2309
HspAI	GCGC	2 cut(s)	920, 1421
Kzo9I	GATC	8 cut(s)	44, 481, 724, 781, 1289, 1633, 2173, 2183
LmnI	GCTCC	6 cut(s)	103, 722, 944, 1428, 2051, 2269
Lsp1109I	GCAGC	7 cut(s)	503, 904, 1073, 1168, 1207, 1333, 1430
LweI	GCATC	5 cut(s)	470, 1045, 1405, 1730, 1813
MaeI	CTAG	2 cut(s)	954, 2294
MaeIII	GTNAC	5 cut(s)	104, 248, 649, 1181, 1916
MalI	GATC	8 cut(s)	46, 483, 726, 783, 1291, 1635, 2175, 2185
MbiI	CCGCTC	1 cut(s)	1684
MboI	GATC	8 cut(s)	44, 481, 724, 781, 1289, 1633, 2173, 2183
MfeI	CAATTG	3 cut(s)	1612, 1733, 2130
MflI	RGATCY	2 cut(s)	1289, 1633
MhlI	GDGCHC	3 cut(s)	160, 719, 941
MlyI	GAGTC	2 cut(s)	901, 2237
MmeI	TCCRAC	2 cut(s)	1551, 2029
Mph1103I	ATGCAT	3 cut(s)	463, 1060, 1476
MroXI	GAANNNNTTC	1 cut(s)	181
MslI	CAYNNNNRTG	4 cut(s)	353, 620, 1053, 1479
MspA1I	CMGCKG	5 cut(s)	362, 527, 971, 1086, 1159
MspI	CCGG	3 cut(s)	330, 1196, 1903
MspR9I	CCNGG	3 cut(s)	331, 1196, 1903
MunI	CAATTG	3 cut(s)	1612, 1733, 2130
Mva1269I	GAATGC	3 cut(s)	349, 463, 1624
MvnI	CGCG	1 cut(s)	2333
MwoI	GCNNNNNNNGC	7 cut(s)	40, 683, 870, 1229, 1343, 1887, 2110
NciI	CCSGG	3 cut(s)	331, 1196, 1903
NcoI	CCATGG	1 cut(s)	1655
NdeII	GATC	8 cut(s)	44, 481, 724, 781, 1289, 1633, 2173, 2183
NmuCI	GTSAC	2 cut(s)	104, 248
NsiI	ATGCAT	3 cut(s)	463, 1060, 1476
NspI	RCATGY	1 cut(s)	2223
NspV	TTCGAA	1 cut(s)	2005
PaeR7I	CTCGAG	1 cut(s)	1846
PagI	TCATGA	2 cut(s)	727, 1525
PceI	AGGCCT	1 cut(s)	1909
PciI	ACATGT	1 cut(s)	2219
PctI	GAATGC	3 cut(s)	349, 463, 1624
PdmI	GAANNNNTTC	1 cut(s)	181
PfeI	GAWTC	6 cut(s)	182, 521, 854, 947, 2152, 2169
PflFI	GACNNNGTC	1 cut(s)	538
PleI	GAGTC	2 cut(s)	900, 2237
PpsI	GAGTC	2 cut(s)	900, 2237
PscI	ACATGT	1 cut(s)	2219
PshBI	ATTAAT	2 cut(s)	498, 554
PspEI	GGTNACC	1 cut(s)	649
PspPI	GGNCC	3 cut(s)	9, 1757, 2033
PspXI	VCTCGAGB	1 cut(s)	1846
PstI	CTGCAG	2 cut(s)	631, 1158
PsuI	RGATCY	2 cut(s)	1289, 1633
PsyI	GACNNNGTC	1 cut(s)	538
PvuII	CAGCTG	3 cut(s)	527, 1086, 1159
RsaI	GTAC	2 cut(s)	1332, 2260
RsaNI	GTAC	2 cut(s)	1331, 2259
RseI	CAYNNNNRTG	4 cut(s)	353, 620, 1053, 1479
SalI	GTCGAC	1 cut(s)	541
Sau3AI	GATC	8 cut(s)	44, 481, 724, 781, 1289, 1633, 2173, 2183
Sau96I	GGNCC	3 cut(s)	9, 1757, 2033
ScaI	AGTACT	1 cut(s)	2260
SchI	GAGTC	2 cut(s)	901, 2237
ScrFI	CCNGG	3 cut(s)	331, 1196, 1903
SduI	GDGCHC	3 cut(s)	160, 719, 941
SfaNI	GCATC	5 cut(s)	470, 1045, 1405, 1730, 1813
SfcI	CTRYAG	5 cut(s)	627, 1154, 1227, 2286, 2411
Sfr274I	CTCGAG	1 cut(s)	1846
SfuI	TTCGAA	1 cut(s)	2005
SinI	GGWCC	2 cut(s)	9, 1757
SlaI	CTCGAG	1 cut(s)	1846
SmiMI	CAYNNNNRTG	4 cut(s)	353, 620, 1053, 1479
SmlI	CTYRAG	5 cut(s)	762, 889, 1032, 1435, 1846
SmoI	CTYRAG	5 cut(s)	762, 889, 1032, 1435, 1846
SseBI	AGGCCT	1 cut(s)	1909
SsiI	CCGC	7 cut(s)	40, 360, 684, 969, 1684, 2102, 2333
SspI	AATATT	1 cut(s)	913
SspMI	CTAG	2 cut(s)	954, 2294
StuI	AGGCCT	1 cut(s)	1909
StyD4I	CCNGG	3 cut(s)	329, 1194, 1901
StyI	CCWWGG	3 cut(s)	942, 1655, 2198
TaaI	ACNGT	6 cut(s)	227, 987, 1481, 1701, 2110, 2122
TaqI	TCGA	6 cut(s)	231, 542, 723, 1847, 2005, 2172
TatI	WGTACW	2 cut(s)	1330, 2258
TauI	GCSGC	3 cut(s)	43, 686, 971
TfiI	GAWTC	6 cut(s)	182, 521, 854, 947, 2152, 2169
TscAI	CASTG	3 cut(s)	355, 1486, 2078
TseFI	GTSAC	2 cut(s)	104, 248
TseI	GCWGC	7 cut(s)	516, 917, 1086, 1156, 1220, 1346, 1418
Tsp45I	GTSAC	2 cut(s)	104, 248
TspGWI	ACGGA	3 cut(s)	474, 809, 1255
TspRI	CASTG	3 cut(s)	355, 1486, 2078
Tth111I	GACNNNGTC	1 cut(s)	538
VneI	GTGCAC	1 cut(s)	156
VpaK11BI	GGWCC	2 cut(s)	9, 1757
VspI	ATTAAT	2 cut(s)	498, 554
XagI	CCTNNNNNAGG	2 cut(s)	807, 1849
XapI	RAATTY	5 cut(s)	1064, 1130, 1403, 1973, 2062
XceI	RCATGY	1 cut(s)	2223
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1808
XhoI	CTCGAG	1 cut(s)	1846
XmiI	GTMKAC	1 cut(s)	542
XmnI	GAANNNNTTC	1 cut(s)	181
XspI	CTAG	2 cut(s)	954, 2294
ZrmI	AGTACT	1 cut(s)	2260
Zsp2I	ATGCAT	3 cut(s)	463, 1060, 1476

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.