Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0006411

Physical Location & Seq

Genomic Coordinates Reverse (-)

49141192 .. 49145924

Gene Structure 4733 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025914

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2691 bp

ATGAGAGAGGAGGGTTCTCGCCGGCCGACGACGTCGTTTAGCTCCGGGTTTACTGTCTTGAAAATCAAAGTGCTTTTAACATACAAGCGAAAGCGGCACTCAAGAAATGATCTTCTACAGGAAGATGGGTGTCATAATTCACCTTCTGACGCTAATGAGGATACTTATTTATCTAGGCCGGACATGCAAGTGCATTTGATTGATGAGAATGCATCAGAAGAATATGAAACAAAGACTACAGTATGCCGTCTATGTGTTGTATGCTGTGTCGGAGGTAACCTGACACACTGTGATAAATGTCTTCAGTCTTATCATCTTGAATGCCTAGATAAGTCCCTCAAGCATACTGATCATGGAACATTGCTAAGCTGTGGGTGTATTGCTGCTTCATTGGGTGATGATGGTAATGTGGGAAGGGGGGCAGTTAGCTTTGATGATAGTTCATTAAGGTTGAATTCATCAAACTTGGAAGAGACTGAGTTGTTTGCTGAAGTTAAGTTAGAGAACTCATGTGATGATCTTTCTGTACAAACCAAGTGGAAAACTCCATTACTTACTTTCCACCGGAGATATAAAAGAAAAAAAGATATGGATGGGTCTCACATACAAAGCAAATCACTGAATGTGGAAAATAACTGTTCAGTGATAACAGAATCAAAAAATTCTGCATGTGCCAAAACCACTTCATGTGGAGCAACTTCCCTTGAAAGCTTCTCACTAGATCATGGAGCAGATTTAAACCCCTGCAAAGAGGTTATTGATATTACATATTCTTATGCTGGACCGGCTCCTGGGTCTAAAATTTTGATGCGTGAAGAGAAGACAATGAATGAAGCTTTACAGCAAGCAGGAAAAGTTCCTGACCAAAGTGCTCAAACTTTGATGGATGTCAAAAAAGAGTCCGTGGATCATTTAGGAACCTCATTAAGTTGTGGTATGAACGACTCCTCTTCTGAAACTGTACTGTTGTGTACACAACCAGGAATAAATTCTCCATTGGTGAAAGAGGAACCTCAAGTTTTGTCAAGTGATGATGATCATAAAGCAGCAGCAACCCCGCATTCGGGCAGATCCCTTCCTAATTTGAACCTGTCTGTGATCACCACTGATTCAAACAGTAGTACTATGGACTTGAATGTTGACTTAAATTTGAGTTCTCAAGAGCAACCTCTTCTTGCTGCGCCAAAAGCAACTCTGGACTCCTTTGAGTCTACTAGCAGAAGTCCTGCCACTGTCTTACATGAACCACCTCCTTCAGAAATAGTGGCCGTCAAAAATGAGAGGATAGAAACTCATGCTTCTCTGTTACGCAAAGATGCATTTGAGTTTTTAGAAGTTGGTGCCAGCTGCATAGATTATGATCAAGTTACTCCTAACTTTGATTCAAAAAACAAATGTCTGCAGTTATTTTCAGAAGAAAAAACCAGCGACATTTCCCACCCAGTGATAATCCAGCCTGAGATGACTGCTTATATGGCCTCAGAGGAGAGAAAAATGCTCCAGTTGGGAAGCAACAATAATCAACCGAATCAAGGATCTCCACTGAATCTAGGTCTATCCTTACCAACTAAACCTTCAACTGCAGGATCTGCAACCAACACCTGTTTCAATACATTTCCTTTCTTCAACTCCTTTACTGGAACCAGAGAATTTATCCAAGATGCAGCACTCCAATCTTCGTCAAGCCATTTATCATCAGTTTTGAGATACAAGATGATGCATGATAGCATTGCAAGCCAAGCAAGAGCTTTGAATGAAATGGGCAGTTTCGATGACATATCTCAGCCATATAATACCATGTGGTCCGAAGAGGAGTTGGATTTTCTATGGATAGGTGTGAGGAGATATGGACGGGACAATTGGAATGCTATGTTAACGGATCCGAGATTGCACTTCGCATCATGGAGGGTGGCAAGGGACTTAGCTATGCGGTGGAAAGAGGAACAGTCAAAACTTTTGAGTAGCATGTTTGGTCCACAAGTCAAGTATTCAAGAGCACAAGGCAGTTCTTTGTACCACAACAACTTCTTGGGTCCACAAACAGGATTCTGGCAACAAAACCCAGCAGATGAAACCCGACTCTCCCTTCGTGTTGTAGATTCTTGTAGAGGAGGTAATGTCTCGAGGAGGCCACTATTCAGATCAACTTATGTTTGCAACGATGGCCAGGAACACCTCTACAGGCCTCTTAGTTACCCGAAGAGGGTATCTCGTCGAAGGATCAAATATGATGAAGATGAATTTCGTAGTTCAAGTAGAAGTCGGAGTATGCTAAGGAACAAGCTGTTGTCAACTGAACTTCCCTCAACTTGCAGTATTGGAGTGAACGGTAGTATGCCCCAGTGGCTCCAAGAATCTGCTGTCGCTTCTCCTCCAAGTCTCAGGGCATCAATTCTGCCTTCAACCGCTTCATCTTTTGCGTTTGCCCATTCTGATACATTGAATGTCACCCGCCCTGATTTCGATCCCTCTGAATCACGTTGTGCAGAAAGGAAGGAAATGCAGTATACATTTGGTGGTTCAAGAGAATATGACCTACAGCCATTAGTAAGTAGTGCTCCTCGTTGCAATTCAGGAGTAAGACCTGAAATGGCTAAACCGAGAAGGGATTCTTCTCATGACGCTGGTAAACAAGATGATTTGATTGTTATAGACAGTGACGCTTCTTCTGAAGGGACCATATCCGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

897

Amino Acids

99.42

Weight (kDa)

5.67

Isoelectric Point (pI)

62.46

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006411)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g00980 FvH4_6g00980 FvH4_6g00980
malus_domestica	MD04G1165900.v1.1 MD04G1239500.v1.1 MD12G1256600.v1.1
prunus_persica	Prupe.3G301900_v2.0.a1 Prupe.3G302000_v2.0.a1 Prupe.6G052100_v2.0.a1 Prupe.6G357400_v2.0.a1 Prupe.6G357700_v2.0.a1
pyrus_communis	pycom04g14760 pycom04g21150 pycom12g23530
rosa_chinensis	RchiOBHm_Chr3g0448441
rosa_laevigata	RLG00000025914
rosa_multiflora	Rmu_sc0003308.1_g000010 Rmu_sc0011979.1_g000002 Rmu_sc0024371.1_g000001 Rmu_sc0027902.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426630
rosa_rugosa	Rorug02G0612500
rosa_samantha	Rh3AG011900 Rh3BG011700 Rh3CG011400 Rh3DG012100
rosa_wichuraiana	Rw3G000920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	35
AccB1I	GGYRCC	1 cut(s)	1340
AccI	GTMKAC	2 cut(s)	1211, 2507
AciI	CCGC	5 cut(s)	94, 1058, 1930, 2406, 2452
AclWI	GGATC	8 cut(s)	915, 1065, 1543, 1594, 1874, 1887, 2228, 2459
AcoI	YGGCCR	3 cut(s)	23, 1266, 2164
AcsI	RAATTY	7 cut(s)	454, 661, 801, 988, 1147, 1649, 2240
AcuI	CTGAAG	4 cut(s)	287, 510, 1239, 2691
AcyI	GRCGYC	1 cut(s)	32
AdeI	CACNNNGTG	3 cut(s)	290, 1444, 2483
AfaI	GTAC	5 cut(s)	528, 963, 973, 1123, 2015
AfiI	CCNNNNNNNGG	6 cut(s)	791, 1063, 1064, 1532, 2042, 2203
AjnI	CCWGG	3 cut(s)	790, 979, 2166
AjuI	GAANNNNNNNTTGG	4 cut(s)	1558, 1589, 1590, 1621
AluBI	AGCT	9 cut(s)	42, 369, 429, 711, 836, 1347, 1748, 1925, 2284
AluI	AGCT	9 cut(s)	42, 369, 429, 711, 836, 1347, 1748, 1925, 2284
Alw21I	GWGCWC	3 cut(s)	874, 1999, 2560
Alw26I	GTCTC	4 cut(s)	467, 603, 2125, 2384
AlwI	GGATC	8 cut(s)	915, 1065, 1543, 1594, 1874, 1887, 2228, 2459
AlwNI	CAGNNNCTG	1 cut(s)	1589
Ama87I	CYCGRG	1 cut(s)	2122
AoxI	GGCC	7 cut(s)	23, 176, 1266, 1476, 2129, 2164, 2183
ApeKI	GCWGC	6 cut(s)	383, 1046, 1049, 1178, 1347, 1664
ApoI	RAATTY	7 cut(s)	454, 661, 801, 988, 1147, 1649, 2240
ArsI	GACNNNNNNTTYG	2 cut(s)	2070, 2102
Asp700I	GAANNNNTTC	2 cut(s)	988, 2608
AspLEI	GCGC	1 cut(s)	1183
AspS9I	GGNCC	5 cut(s)	782, 1803, 1973, 2033, 2676
AsuC2I	CCSGG	1 cut(s)	46
AsuHPI	GGTGA	5 cut(s)	132, 407, 1012, 1093, 2440
AvaI	CYCGRG	1 cut(s)	2122
AvaII	GGWCC	5 cut(s)	782, 1803, 1973, 2033, 2676
BalI	TGGCCA	1 cut(s)	2166
BamHI	GGATCC	1 cut(s)	1879
BanI	GGYRCC	1 cut(s)	1340
BarI	GAAGNNNNNNTAC	2 cut(s)	370, 402
BbsI	GAAGAC	2 cut(s)	293, 827
Bbv12I	GWGCWC	3 cut(s)	874, 1999, 2560
BbvI	GCAGC	6 cut(s)	370, 1058, 1061, 1165, 1334, 1676
BccI	CCATC	5 cut(s)	119, 395, 587, 877, 2156
BceAI	ACGGC	2 cut(s)	231, 1253
BciT130I	CCWGG	3 cut(s)	792, 981, 2168
BciVI	GTATCC	1 cut(s)	154
BclI	TGATCA	4 cut(s)	349, 1036, 1098, 1360
BcnI	CCSGG	1 cut(s)	46
BcoDI	GTCTC	4 cut(s)	467, 603, 2125, 2384
BfaI	CTAG	5 cut(s)	174, 326, 719, 1215, 1550
BfmI	CTRYAG	6 cut(s)	116, 237, 1400, 1581, 2179, 2537
BfuI	GTATCC	1 cut(s)	154
BglI	GCCNNNNNGGC	1 cut(s)	2344
BisI	GCNGC	7 cut(s)	95, 384, 1047, 1050, 1179, 1348, 1665
BlpI	GCTNAGC	1 cut(s)	365
BlsI	GCNGC	7 cut(s)	96, 385, 1048, 1051, 1180, 1349, 1666
BmcAI	AGTACT	1 cut(s)	1123
Bme1390I	CCNGG	4 cut(s)	46, 792, 981, 2168
Bme18I	GGWCC	5 cut(s)	782, 1803, 1973, 2033, 2676
BmeT110I	CYCGRG	1 cut(s)	2122
BmgT120I	GGNCC	5 cut(s)	782, 1803, 1973, 2033, 2676
BmiI	GGNNCC	9 cut(s)	789, 919, 1011, 1342, 1642, 1881, 2034, 2348, 2677
BmrFI	CCNGG	4 cut(s)	46, 792, 981, 2168
BmrI	ACTGGG	2 cut(s)	1436, 2335
BmsI	GCATC	7 cut(s)	221, 798, 1306, 1651, 1707, 1907, 2396
BmuI	ACTGGG	2 cut(s)	1436, 2335
BpiI	GAAGAC	2 cut(s)	293, 827
BplI	GAGNNNNNCTC	2 cut(s)	2194, 2226
BpmI	CTGGAG	1 cut(s)	1484
Bpu10I	CCTNAGC	1 cut(s)	2273
Bpu1102I	GCTNAGC	1 cut(s)	365
BpuEI	CTTGAG	4 cut(s)	85, 323, 999, 1143
BpuMI	CCSGG	1 cut(s)	46
BsaBI	GATNNNNATC	3 cut(s)	1035, 1359, 2463
BsaHI	GRCGYC	1 cut(s)	32
BsaI	GGTCTC	1 cut(s)	603
BsaJI	CCNNGG	2 cut(s)	791, 903
BsaWI	WCCGGW	1 cut(s)	564
BsaXI	ACNNNNNCTCC	2 cut(s)	2103, 2133
Bsc4I	CCNNNNNNNGG	6 cut(s)	791, 1063, 1064, 1532, 2042, 2203
Bse118I	RCCGGY	2 cut(s)	21, 784
Bse1I	ACTGG	4 cut(s)	1442, 1501, 1642, 2341
Bse3DI	GCAATG	2 cut(s)	359, 1728
Bse8I	GATNNNNATC	3 cut(s)	1035, 1359, 2463
BseBI	CCWGG	3 cut(s)	792, 981, 2168
BseDI	CCNNGG	2 cut(s)	791, 903
BseGI	GGATG	2 cut(s)	598, 892
BseJI	GATNNNNATC	3 cut(s)	1035, 1359, 2463
BseLI	CCNNNNNNNGG	6 cut(s)	791, 1063, 1064, 1532, 2042, 2203
BseMI	GCAATG	2 cut(s)	359, 1728
BseMII	CTCAG	5 cut(s)	468, 1449, 1494, 1796, 2395
BseNI	ACTGG	4 cut(s)	1442, 1501, 1642, 2341
BseRI	GAGGAG	9 cut(s)	23, 937, 1499, 1826, 1855, 2124, 2140, 2361, 2550
BseX3I	CGGCCG	1 cut(s)	23
BseXI	GCAGC	6 cut(s)	370, 1058, 1061, 1165, 1334, 1676
BseYI	CCCAGC	1 cut(s)	2062
BsgI	GTGCAG	1 cut(s)	2505
Bsh1285I	CGRYCG	1 cut(s)	26
BshFI	GGCC	7 cut(s)	25, 178, 1268, 1478, 2131, 2166, 2185
BshNI	GGYRCC	1 cut(s)	1340
BsiEI	CGRYCG	1 cut(s)	26
BsiHKAI	GWGCWC	3 cut(s)	874, 1999, 2560
BsiHKCI	CYCGRG	1 cut(s)	2122
BsiSI	CCGG	5 cut(s)	22, 45, 179, 565, 785
BslFI	GGGAC	3 cut(s)	319, 1868, 1931
BslI	CCNNNNNNNGG	6 cut(s)	791, 1063, 1064, 1532, 2042, 2203
BsmAI	GTCTC	4 cut(s)	467, 603, 2125, 2384
BsmFI	GGGAC	3 cut(s)	319, 1868, 1931
BsmI	GAATGC	4 cut(s)	214, 326, 1060, 1870
BsnI	GGCC	7 cut(s)	25, 178, 1268, 1478, 2131, 2166, 2185
Bso31I	GGTCTC	1 cut(s)	603
BsoBI	CYCGRG	1 cut(s)	2122
Bsp1286I	GDGCHC	3 cut(s)	874, 1999, 2560
Bsp1407I	TGTACA	2 cut(s)	526, 971
Bsp1720I	GCTNAGC	1 cut(s)	365
BspACI	CCGC	5 cut(s)	94, 1058, 1930, 2406, 2452
BspANI	GGCC	7 cut(s)	25, 178, 1268, 1478, 2131, 2166, 2185
BspCNI	CTCAG	5 cut(s)	469, 1450, 1493, 1795, 2394
BspHI	TCATGA	1 cut(s)	2617
BspLI	GGNNCC	9 cut(s)	789, 919, 1011, 1342, 1642, 1881, 2034, 2348, 2677
BspMAI	CTGCAG	2 cut(s)	1404, 1585
BspPI	GGATC	8 cut(s)	915, 1065, 1543, 1594, 1874, 1887, 2228, 2459
BspT107I	GGYRCC	1 cut(s)	1340
BspTNI	GGTCTC	1 cut(s)	603
BsrDI	GCAATG	2 cut(s)	359, 1728
BsrFI	RCCGGY	2 cut(s)	21, 784
BsrGI	TGTACA	2 cut(s)	526, 971
BsrI	ACTGG	4 cut(s)	1442, 1501, 1642, 2341
BssAI	RCCGGY	2 cut(s)	21, 784
BssECI	CCNNGG	2 cut(s)	791, 903
BssNAI	GTATAC	1 cut(s)	2508
BssNI	GRCGYC	1 cut(s)	32
Bst1107I	GTATAC	1 cut(s)	2508
Bst2UI	CCWGG	3 cut(s)	792, 981, 2168
Bst6I	CTCTTC	6 cut(s)	465, 810, 955, 1176, 1803, 2195
BstACI	GRCGYC	1 cut(s)	32
BstAPI	GCANNNNNTGC	1 cut(s)	1589
BstAUI	TGTACA	2 cut(s)	526, 971
BstC8I	GCNNGC	4 cut(s)	23, 846, 1345, 1735
BstDEI	CTNAG	9 cut(s)	365, 477, 1458, 1480, 1782, 1921, 2189, 2273, 2381
BstDSI	CCRYGG	1 cut(s)	903
BstEII	GGTNACC	1 cut(s)	275
BstF5I	GGATG	2 cut(s)	598, 892
BstHHI	GCGC	1 cut(s)	1183
BstMAI	GTCTC	4 cut(s)	467, 603, 2125, 2384
BstMCI	CGRYCG	1 cut(s)	26
BstNI	CCWGG	3 cut(s)	792, 981, 2168
BstNSI	RCATGY	3 cut(s)	187, 672, 1969
BstPI	GGTNACC	1 cut(s)	275
BstSCI	CCNGG	4 cut(s)	44, 790, 979, 2166
BstSFI	CTRYAG	6 cut(s)	116, 237, 1400, 1581, 2179, 2537
BstV1I	GCAGC	6 cut(s)	370, 1058, 1061, 1165, 1334, 1676
BstV2I	GAAGAC	2 cut(s)	293, 827
BstX2I	RGATCY	4 cut(s)	1070, 1535, 1586, 1879
BstYI	RGATCY	4 cut(s)	1070, 1535, 1586, 1879
BstZ17I	GTATAC	1 cut(s)	2508
BstZI	CGGCCG	1 cut(s)	23
BsuI	GTATCC	1 cut(s)	154
BsuRI	GGCC	7 cut(s)	25, 178, 1268, 1478, 2131, 2166, 2185
BtgI	CCRYGG	1 cut(s)	903
BtsCI	GGATG	2 cut(s)	598, 892
BtsIMutI	CAGTG	9 cut(s)	286, 617, 648, 1104, 1230, 1449, 1541, 2348, 2662
Cac8I	GCNNGC	4 cut(s)	23, 846, 1345, 1735
CaiI	CAGNNNCTG	1 cut(s)	1589
CciI	TCATGA	1 cut(s)	2617
CfoI	GCGC	1 cut(s)	1183
Cfr10I	RCCGGY	2 cut(s)	21, 784
Cfr13I	GGNCC	5 cut(s)	782, 1803, 1973, 2033, 2676
CseI	GACGC	3 cut(s)	158, 2630, 2669
Csp6I	GTAC	5 cut(s)	527, 962, 972, 1122, 2014
CspCI	CAANNNNNGTGG	2 cut(s)	518, 553
CviQI	GTAC	5 cut(s)	527, 962, 972, 1122, 2014
DdeI	CTNAG	9 cut(s)	365, 477, 1458, 1480, 1782, 1921, 2189, 2273, 2381
DraI	TTTAAA	1 cut(s)	738
DraIII	CACNNNGTG	3 cut(s)	290, 1444, 2483
EaeI	YGGCCR	3 cut(s)	23, 1266, 2164
EagI	CGGCCG	1 cut(s)	23
Eam1104I	CTCTTC	6 cut(s)	465, 810, 955, 1176, 1803, 2195
EarI	CTCTTC	6 cut(s)	465, 810, 955, 1176, 1803, 2195
EclXI	CGGCCG	1 cut(s)	23
Eco147I	AGGCCT	1 cut(s)	2185
Eco31I	GGTCTC	1 cut(s)	603
Eco47I	GGWCC	5 cut(s)	782, 1803, 1973, 2033, 2676
Eco52I	CGGCCG	1 cut(s)	23
Eco57I	CTGAAG	4 cut(s)	287, 510, 1239, 2691
Eco88I	CYCGRG	1 cut(s)	2122
Eco91I	GGTNACC	1 cut(s)	275
EcoO65I	GGTNACC	1 cut(s)	275
EcoRI	GAATTC	1 cut(s)	454
EcoRII	CCWGG	3 cut(s)	790, 979, 2166
EcoT22I	ATGCAT	3 cut(s)	214, 1321, 1722
FalI	AAGNNNNNCTT	2 cut(s)	2596, 2628
FaqI	GGGAC	3 cut(s)	319, 1868, 1931
FauI	CCCGC	2 cut(s)	1065, 2459
FbaI	TGATCA	4 cut(s)	349, 1036, 1098, 1360
FblI	GTMKAC	2 cut(s)	1211, 2507
Fnu4HI	GCNGC	7 cut(s)	95, 384, 1047, 1050, 1179, 1348, 1665
FokI	GGATG	2 cut(s)	605, 899
Fsp4HI	GCNGC	7 cut(s)	95, 384, 1047, 1050, 1179, 1348, 1665
FspBI	CTAG	5 cut(s)	174, 326, 719, 1215, 1550
GlaI	GCGC	1 cut(s)	1182
GluI	GCNGC	7 cut(s)	95, 384, 1047, 1050, 1179, 1348, 1665
GsaI	CCCAGC	1 cut(s)	2066
GsuI	CTGGAG	1 cut(s)	1484
HaeIII	GGCC	7 cut(s)	25, 178, 1268, 1478, 2131, 2166, 2185
HapII	CCGG	5 cut(s)	22, 45, 179, 565, 785
HgaI	GACGC	3 cut(s)	158, 2630, 2669
HhaI	GCGC	1 cut(s)	1183
Hin1I	GRCGYC	1 cut(s)	32
Hin6I	GCGC	1 cut(s)	1181
HinP1I	GCGC	1 cut(s)	1181
HincII	GTYRAC	3 cut(s)	1141, 1875, 2292
HindII	GTYRAC	3 cut(s)	1141, 1875, 2292
HindIII	AAGCTT	2 cut(s)	709, 834
HpaI	GTTAAC	1 cut(s)	1875
HpaII	CCGG	5 cut(s)	22, 45, 179, 565, 785
HphI	GGTGA	5 cut(s)	132, 407, 1012, 1093, 2440
Hpy99I	CGWCG	4 cut(s)	31, 34, 37, 2217
HpyCH4IV	ACGT	2 cut(s)	32, 2479
HpyF3I	CTNAG	9 cut(s)	365, 477, 1458, 1480, 1782, 1921, 2189, 2273, 2381
HpySE526I	ACGT	2 cut(s)	32, 2479
Hsp92I	GRCGYC	1 cut(s)	32
HspAI	GCGC	1 cut(s)	1181
KroI	GCCGGC	1 cut(s)	21
KroNI	GCCGGC	1 cut(s)	23
Ksp22I	TGATCA	4 cut(s)	349, 1036, 1098, 1360
KspAI	GTTAAC	1 cut(s)	1875
LmnI	GCTCC	7 cut(s)	47, 692, 728, 793, 1503, 2352, 2563
Lsp1109I	GCAGC	6 cut(s)	370, 1058, 1061, 1165, 1334, 1676
LweI	GCATC	7 cut(s)	221, 798, 1306, 1651, 1707, 1907, 2396
MaeI	CTAG	5 cut(s)	174, 326, 719, 1215, 1550
MaeII	ACGT	2 cut(s)	32, 2479
MaeIII	GTNAC	6 cut(s)	275, 1305, 1366, 2192, 2446, 2657
MfeI	CAATTG	1 cut(s)	1858
MflI	RGATCY	4 cut(s)	1070, 1535, 1586, 1879
MhlI	GDGCHC	3 cut(s)	874, 1999, 2560
MlsI	TGGCCA	1 cut(s)	2166
MluNI	TGGCCA	1 cut(s)	2166
MlyI	GAGTC	5 cut(s)	908, 938, 1193, 1217, 2073
MmeI	TCCRAC	3 cut(s)	250, 1797, 2243
Mox20I	TGGCCA	1 cut(s)	2166
Mph1103I	ATGCAT	3 cut(s)	214, 1321, 1722
MroNI	GCCGGC	1 cut(s)	21
MroXI	GAANNNNTTC	2 cut(s)	988, 2608
MscI	TGGCCA	1 cut(s)	2166
MseI	TTAA	8 cut(s)	77, 446, 495, 737, 926, 1145, 1874, 2689
MslI	CAYNNNNRTG	1 cut(s)	188
Msp20I	TGGCCA	1 cut(s)	2166
MspA1I	CMGCKG	1 cut(s)	1347
MspI	CCGG	5 cut(s)	22, 45, 179, 565, 785
MspR9I	CCNGG	4 cut(s)	46, 792, 981, 2168
MunI	CAATTG	1 cut(s)	1858
Mva1269I	GAATGC	4 cut(s)	214, 326, 1060, 1870
MvaI	CCWGG	3 cut(s)	792, 981, 2168
NaeI	GCCGGC	1 cut(s)	23
NciI	CCSGG	1 cut(s)	46
NgoMIV	GCCGGC	1 cut(s)	21
NlaIV	GGNNCC	9 cut(s)	789, 919, 1011, 1342, 1642, 1881, 2034, 2348, 2677
NmuCI	GTSAC	2 cut(s)	2446, 2657
NsiI	ATGCAT	3 cut(s)	214, 1321, 1722
NspI	RCATGY	3 cut(s)	187, 672, 1969
PaeR7I	CTCGAG	1 cut(s)	2122
PagI	TCATGA	1 cut(s)	2617
PceI	AGGCCT	1 cut(s)	2185
PctI	GAATGC	4 cut(s)	214, 326, 1060, 1870
PdiI	GCCGGC	1 cut(s)	23
PdmI	GAANNNNTTC	2 cut(s)	988, 2608
PflFI	GACNNNGTC	1 cut(s)	31
PkrI	GCNGC	7 cut(s)	96, 385, 1048, 1051, 1180, 1349, 1666
PleI	GAGTC	5 cut(s)	907, 938, 1193, 1216, 2073
PpsI	GAGTC	5 cut(s)	907, 938, 1193, 1216, 2073
Psp6I	CCWGG	3 cut(s)	790, 979, 2166
PspEI	GGTNACC	1 cut(s)	275
PspFI	CCCAGC	1 cut(s)	2062
PspGI	CCWGG	3 cut(s)	790, 979, 2166
PspN4I	GGNNCC	9 cut(s)	789, 919, 1011, 1342, 1642, 1881, 2034, 2348, 2677
PspPI	GGNCC	5 cut(s)	782, 1803, 1973, 2033, 2676
PstI	CTGCAG	2 cut(s)	1404, 1585
PstNI	CAGNNNCTG	1 cut(s)	1589
PsuI	RGATCY	4 cut(s)	1070, 1535, 1586, 1879
PsyI	GACNNNGTC	1 cut(s)	31
PvuII	CAGCTG	1 cut(s)	1347
RsaI	GTAC	5 cut(s)	528, 963, 973, 1123, 2015
RsaNI	GTAC	5 cut(s)	527, 962, 972, 1122, 2014
RseI	CAYNNNNRTG	1 cut(s)	188
SaqAI	TTAA	8 cut(s)	77, 446, 495, 737, 926, 1145, 1874, 2689
SatI	GCNGC	7 cut(s)	95, 384, 1047, 1050, 1179, 1348, 1665
Sau96I	GGNCC	5 cut(s)	782, 1803, 1973, 2033, 2676
ScaI	AGTACT	1 cut(s)	1123
SchI	GAGTC	5 cut(s)	908, 938, 1193, 1217, 2073
ScrFI	CCNGG	4 cut(s)	46, 792, 981, 2168
SduI	GDGCHC	3 cut(s)	874, 1999, 2560
SfaNI	GCATC	7 cut(s)	221, 798, 1306, 1651, 1707, 1907, 2396
SfcI	CTRYAG	6 cut(s)	116, 237, 1400, 1581, 2179, 2537
Sfr274I	CTCGAG	1 cut(s)	2122
SinI	GGWCC	5 cut(s)	782, 1803, 1973, 2033, 2676
SlaI	CTCGAG	1 cut(s)	2122
SmiMI	CAYNNNNRTG	1 cut(s)	188
SmlI	CTYRAG	5 cut(s)	100, 338, 1014, 1158, 2122
SmoI	CTYRAG	5 cut(s)	100, 338, 1014, 1158, 2122
SseBI	AGGCCT	1 cut(s)	2185
SsiI	CCGC	5 cut(s)	94, 1058, 1930, 2406, 2452
SspMI	CTAG	5 cut(s)	174, 326, 719, 1215, 1550
StuI	AGGCCT	1 cut(s)	2185
StyD4I	CCNGG	4 cut(s)	44, 790, 979, 2166
TaiI	ACGT	2 cut(s)	35, 2482
TaqI	TCGA	4 cut(s)	1770, 2123, 2215, 2463
TatI	WGTACW	4 cut(s)	526, 961, 971, 1121
TauI	GCSGC	1 cut(s)	97
Tru1I	TTAA	8 cut(s)	77, 446, 495, 737, 926, 1145, 1874, 2689
Tru9I	TTAA	8 cut(s)	77, 446, 495, 737, 926, 1145, 1874, 2689
TscAI	CASTG	9 cut(s)	293, 624, 648, 1111, 1237, 1449, 1548, 2348, 2662
TseFI	GTSAC	2 cut(s)	2446, 2657
TseI	GCWGC	6 cut(s)	383, 1046, 1049, 1178, 1347, 1664
Tsp45I	GTSAC	2 cut(s)	2446, 2657
TspGWI	ACGGA	2 cut(s)	892, 1892
TspRI	CASTG	9 cut(s)	293, 624, 648, 1111, 1237, 1449, 1548, 2348, 2662
Tth111I	GACNNNGTC	1 cut(s)	31
VpaK11BI	GGWCC	5 cut(s)	782, 1803, 1973, 2033, 2676
XapI	RAATTY	7 cut(s)	454, 661, 801, 988, 1147, 1649, 2240
XceI	RCATGY	3 cut(s)	187, 672, 1969
XhoI	CTCGAG	1 cut(s)	2122
XmiI	GTMKAC	2 cut(s)	1211, 2507
XmnI	GAANNNNTTC	2 cut(s)	988, 2608
XspI	CTAG	5 cut(s)	174, 326, 719, 1215, 1550
ZraI	GACGTC	1 cut(s)	33
ZrmI	AGTACT	1 cut(s)	1123
Zsp2I	ATGCAT	3 cut(s)	214, 1321, 1722

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.