Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006411

Physical Location & Seq

Genomic Coordinates Reverse (-)

31760027 .. 31764352

Gene Structure 4326 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1239500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGTCAGGGACCAAAGTGATTTTAACGTACAAGCGAAAGCGGCAATCAAGGAAAGGTCTTGTACAGGGACATGAGTGCCATAATGCTCTTTTTGTTGCTCCGAGTGATACTTCTCCATGTAGAGAAGACTTGCAAGTTCATTTGATTGAAGAGCGCTCAGCCAAAGACTATAGCAGACTGGAGGATAAGAAACAAGTTAAAGTACCTGAGCCGAGACAATTGACAATTAATTCCCATAAATTTCTGACAAGTCTTGATGAGGGTCCCTCCGAAAGGGATACGTATGGATCCTCTTCTATGGACCTATCATTGGAGAATAAGTCAAGTCACCAGAAAGTTGGCTCGTCCTCAAATACTAATAGTGAAGCGTTGGTTGATGATAATAATCTTGGAGGGAGGTTAGTTTGCGGATTGACAGGGAATGCCACTGAAATGACTGCTGATTTTGTCCGACCAAAATCATTATCATCATCATCATCATTTGAAAGAAAAAGTGGCTCTAAATGTGTTGGTATATCGCTAAGATTGAATGTATCAAACCTGGAAGACACTGATTCACTCTCTAAAAATAAGGTAGATAGCTCATGTGGTGATTCAGCTGTGCAGACCAAGTCGACTGCCCCATTGATTACCTTCTGCCGAAGGAAAAAAAGAAGAAAGGATACAGATAAGTCTGATATACGAAGCAAATCTGTGCCTGTGGAAAACAATTGCTCATTGGTAACCAAATTGAGTAATTCTGTTTGTGCTAATGCCGCTTCGTGTGACGAAACTTCCCCTGAAAACTGCTCAGTAAATCATGAGACAGATTTGAAGCACTCCATAGACGAAGCTCAAGTTTTGTCAAGTGATGATCTTAAAGCAACAGTAATCCCTCGTTTAGGCAGATCACTTCCTTACTTGGACCTGTCTGTTATCCCAACTGATTCATGCGGCACTGTGGACTGCAATTTTGACTTAAACTTGAGTCCTGCTAAGCAACCTGACACCGATGCACCAAAAGCTGTGGGGGACTCGTTGGATTCTACTAGCAGAAATCATGCCTTTGTATTACATAAACCGTCTCCTCCACAAATGTCGGCTGAGAGAACTGGAAGAGTGGAAACTCAAGCTTCACAATTACACAGAGATCGATTTGAATGTTTAGAAGTTGGTGCCAGCTGCAAAGATGATGATGATAAAGTTGGTTCTCTGATTTCCGAGGAGAATACTTCCAAAAATAAATGTCTGCAGCTGTTTCCAGAAGAAAAAACCAGTGGCATTTTTTGCCCGGTGATAACACATCCTGAGGTAGTTGCTTCTGTAGCTTCAGAACGGAGAAAAGTTCTTCAGTTGGGAGGCCAACATGATCAACCAAAACAAGGATCTCCTATGTTTTTGGGTCCATCCTTAACCGAAAAACCTATATTTGCAGGATGTGCTGCCAATACCTGCTTCAATACATCTCCCTTCTTGAACTCCATCATTAGAACAAGAGAATTCATCCGAGATGCAGCGCTCCAATCTTCTTCAAGCCGTTTATCATCAGTTTTGAGGCACAAGCTTATGCATGACGGTGTAGTAAGCCAAGCAAGAGCTTTGAATGAAAGGGGTGGTTTTCATGACAAATATATGCCATATAATACCATTGGCATTTGTGTTCCCCAATTGGGATGCAACTACTTCTCGGGTCCAAAAAGAGGAATATGGAAAGAAAATACAGCAGACATGACCCAAGTTTCACTTGGCGATGTTGATGCTTGTAGAGGAGGTGATGTCTCGAGGAGGCCACCATTCAATTCGGCTTATACTTGCAACAATGGCAATGAAAACCACCGGAGGCCTCTTGGTTACACAAAGAGGACATCTCGTTTTGAAACGGGAAAGGACACACATGAGGATGATGAATTTAGTATTCTAAATAGAAGTAGAAGTATTCGGAGGGGCAAGTTGTTATCGACTGATGTCCCCACAACTTGCACTGGACCACAGGGAAATTTGCCCCACTGGCTCAGAGAAGCTGTTGCTTCTCCACCCCCAACTGTGCCTTCAACTGTTTCATCAATTGCTCATTCAGACAGGTTGAATGTCACCCTTGCTGGTTTTGATCATCGGGAATCACATTTGGCACCAAGGAATGAAATGCAGGTAAATGCTCCTCTTTTCAACTATTCACGACTTGGGACGGGGAAACAGAGAAGGGATCATTCTCGTCGTGCGGGTAAACAAGACAACGTAATTATTATAGCCAGCGATGGTTCTTCTGAAGAGACTATATCTGATGATCGTAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

758

Amino Acids

83.11

Weight (kDa)

8.32

Isoelectric Point (pI)

57.13

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006411)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g00980 FvH4_6g00980 FvH4_6g00980
malus_domestica	MD04G1165900.v1.1 MD04G1239500.v1.1 MD12G1256600.v1.1
prunus_persica	Prupe.3G301900_v2.0.a1 Prupe.3G302000_v2.0.a1 Prupe.6G052100_v2.0.a1 Prupe.6G357400_v2.0.a1 Prupe.6G357700_v2.0.a1
pyrus_communis	pycom04g14760 pycom04g21150 pycom12g23530
rosa_chinensis	RchiOBHm_Chr3g0448441
rosa_laevigata	RLG00000025914
rosa_multiflora	Rmu_sc0003308.1_g000010 Rmu_sc0011979.1_g000002 Rmu_sc0024371.1_g000001 Rmu_sc0027902.1_g000001
rosa_roxburghii	Rroxscaffold_6G00426630
rosa_rugosa	Rorug02G0612500
rosa_samantha	Rh3AG011900 Rh3BG011700 Rh3CG011400 Rh3DG012100
rosa_wichuraiana	Rw3G000920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1439, 2116
AccB1I	GGYRCC	2 cut(s)	1154, 2107
AccI	GTMKAC	1 cut(s)	614
AciI	CCGC	5 cut(s)	40, 408, 756, 933, 2198
AclWI	GGATC	4 cut(s)	282, 295, 1372, 2190
AcsI	RAATTY	4 cut(s)	239, 1478, 1886, 1975
AcuI	CTGAAG	3 cut(s)	1293, 1313, 2265
AfaI	GTAC	3 cut(s)	29, 63, 204
AfeI	AGCGCT	2 cut(s)	155, 1497
AfiI	CCNNNNNNNGG	6 cut(s)	273, 310, 881, 1361, 1649, 1679
AjnI	CCWGG	1 cut(s)	540
AjuI	GAANNNNNNNTTGG	4 cut(s)	1418, 1450, 2011, 2043
Alw26I	GTCTC	5 cut(s)	208, 797, 1068, 1762, 2243
AlwI	GGATC	4 cut(s)	282, 295, 1372, 2190
Ama87I	CYCGRG	2 cut(s)	1666, 1759
Aor51HI	AGCGCT	2 cut(s)	155, 1497
AoxI	GGCC	3 cut(s)	1339, 1766, 1820
ApeKI	GCWGC	4 cut(s)	1161, 1231, 1421, 1493
ApoI	RAATTY	4 cut(s)	239, 1478, 1886, 1975
ArsI	GACNNNNNNTTYG	2 cut(s)	935, 967
AseI	ATTAAT	1 cut(s)	228
AspLEI	GCGC	2 cut(s)	156, 1498
AspS9I	GGNCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
AsuC2I	CCSGG	1 cut(s)	1271
AsuHPI	GGTGA	5 cut(s)	320, 602, 1285, 1763, 2062
AvaI	CYCGRG	2 cut(s)	1666, 1759
AvaII	GGWCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
AxyI	CCTNAGG	1 cut(s)	1287
BamHI	GGATCC	1 cut(s)	287
BanI	GGYRCC	2 cut(s)	1154, 2107
BarI	GAAGNNNNNNTAC	2 cut(s)	646, 678
BbsI	GAAGAC	2 cut(s)	132, 552
BbvI	GCAGC	4 cut(s)	1148, 1243, 1408, 1505
BccI	CCATC	3 cut(s)	1393, 1469, 2228
BceAI	ACGGC	1 cut(s)	1500
BcgI	CGANNNNNNTGC	2 cut(s)	1718, 1752
BciT130I	CCWGG	1 cut(s)	542
BciVI	GTATCC	2 cut(s)	271, 655
BclI	TGATCA	2 cut(s)	1348, 2086
BcnI	CCSGG	1 cut(s)	1271
BcoDI	GTCTC	5 cut(s)	208, 797, 1068, 1762, 2243
BfaI	CTAG	1 cut(s)	1029
BfmI	CTRYAG	3 cut(s)	169, 1229, 1302
BfoI	RGCGCY	2 cut(s)	157, 1499
BfuAI	ACCTGC	2 cut(s)	1439, 2116
BfuI	GTATCC	2 cut(s)	271, 655
BglI	GCCNNNNNGGC	1 cut(s)	1987
BisI	GCNGC	7 cut(s)	41, 756, 934, 1162, 1232, 1422, 1494
BlpI	GCTNAGC	2 cut(s)	157, 975
BlsI	GCNGC	7 cut(s)	42, 757, 935, 1163, 1233, 1423, 1495
Bme1390I	CCNGG	2 cut(s)	542, 1271
Bme18I	GGWCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
BmeT110I	CYCGRG	2 cut(s)	1666, 1759
BmgT120I	GGNCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
BmiI	GGNNCC	8 cut(s)	10, 264, 265, 289, 1156, 1383, 1671, 2109
BmrFI	CCNGG	2 cut(s)	542, 1271
BmsI	GCATC	4 cut(s)	982, 1480, 1643, 1726
BoxI	GACNNNNGTC	1 cut(s)	1943
BpiI	GAAGAC	2 cut(s)	132, 552
BplI	GAGNNNNNCTC	4 cut(s)	251, 283, 1831, 1863
BpmI	CTGGAG	1 cut(s)	200
Bpu10I	CCTNAGC	1 cut(s)	207
Bpu1102I	GCTNAGC	2 cut(s)	157, 975
BpuEI	CTTGAG	3 cut(s)	819, 985, 1092
BpuMI	CCSGG	1 cut(s)	1271
Bsa29I	ATCGAT	1 cut(s)	1132
BsaAI	YACGTR	1 cut(s)	282
BsaBI	GATNNNNATC	1 cut(s)	384
BsaJI	CCNNGG	2 cut(s)	1200, 2111
BsaWI	WCCGGW	1 cut(s)	1815
BsaXI	ACNNNNNCTCC	4 cut(s)	305, 335, 1740, 1770
Bsc4I	CCNNNNNNNGG	6 cut(s)	273, 310, 881, 1361, 1649, 1679
Bse1I	ACTGG	5 cut(s)	183, 1096, 1254, 1966, 1991
Bse21I	CCTNAGG	1 cut(s)	1287
Bse3DI	GCAATG	1 cut(s)	1810
Bse8I	GATNNNNATC	1 cut(s)	384
BseBI	CCWGG	1 cut(s)	542
BseCI	ATCGAT	1 cut(s)	1132
BseDI	CCNNGG	2 cut(s)	1200, 2111
BseGI	GGATG	6 cut(s)	1282, 1385, 1421, 1482, 1658, 1885
BseJI	GATNNNNATC	1 cut(s)	384
BseLI	CCNNNNNNNGG	6 cut(s)	273, 310, 881, 1361, 1649, 1679
BseMI	GCAATG	1 cut(s)	1810
BseMII	CTCAG	6 cut(s)	171, 198, 804, 1074, 1278, 2005
BseNI	ACTGG	5 cut(s)	183, 1096, 1254, 1966, 1991
BseRI	GAGGAG	5 cut(s)	1056, 1217, 1761, 1777, 2127
BseXI	GCAGC	4 cut(s)	1148, 1243, 1408, 1505
BsgI	GTGCAG	1 cut(s)	623
BshFI	GGCC	3 cut(s)	1341, 1768, 1822
BshNI	GGYRCC	2 cut(s)	1154, 2107
BshVI	ATCGAT	1 cut(s)	1132
BsiHKCI	CYCGRG	2 cut(s)	1666, 1759
BsiSI	CCGG	2 cut(s)	1271, 1816
BslFI	GGGAC	6 cut(s)	22, 81, 249, 1025, 1931, 2176
BslI	CCNNNNNNNGG	6 cut(s)	273, 310, 881, 1361, 1649, 1679
BsmAI	GTCTC	5 cut(s)	208, 797, 1068, 1762, 2243
BsmBI	CGTCTC	1 cut(s)	1068
BsmFI	GGGAC	6 cut(s)	22, 81, 249, 1025, 1931, 2176
BsmI	GAATGC	1 cut(s)	427
BsnI	GGCC	3 cut(s)	1341, 1768, 1822
BsoBI	CYCGRG	2 cut(s)	1666, 1759
Bsp1407I	TGTACA	1 cut(s)	61
Bsp143I	GATC	9 cut(s)	287, 853, 887, 1129, 1348, 1364, 2086, 2182, 2263
Bsp1720I	GCTNAGC	2 cut(s)	157, 975
BspACI	CCGC	5 cut(s)	40, 408, 756, 933, 2198
BspANI	GGCC	3 cut(s)	1341, 1768, 1822
BspCNI	CTCAG	6 cut(s)	170, 199, 803, 1075, 1279, 2004
BspDI	ATCGAT	1 cut(s)	1132
BspHI	TCATGA	2 cut(s)	799, 1600
BspLI	GGNNCC	8 cut(s)	10, 264, 265, 289, 1156, 1383, 1671, 2109
BspMAI	CTGCAG	1 cut(s)	1233
BspMI	ACCTGC	2 cut(s)	1439, 2116
BspPI	GGATC	4 cut(s)	282, 295, 1372, 2190
BspQI	GCTCTTC	1 cut(s)	144
BspT107I	GGYRCC	2 cut(s)	1154, 2107
BsrDI	GCAATG	1 cut(s)	1810
BsrGI	TGTACA	1 cut(s)	61
BsrI	ACTGG	5 cut(s)	183, 1096, 1254, 1966, 1991
BssECI	CCNNGG	2 cut(s)	1200, 2111
BssMI	GATC	9 cut(s)	287, 853, 887, 1129, 1348, 1364, 2086, 2182, 2263
BssT1I	CCWWGG	1 cut(s)	2111
Bst2UI	CCWGG	1 cut(s)	542
Bst4CI	ACNGT	6 cut(s)	868, 940, 1062, 1556, 2023, 2035
Bst6I	CTCTTC	4 cut(s)	144, 298, 1090, 2241
BstAPI	GCANNNNNTGC	2 cut(s)	1266, 1418
BstAUI	TGTACA	1 cut(s)	61
BstBAI	YACGTR	1 cut(s)	282
BstC8I	GCNNGC	2 cut(s)	1159, 2230
BstDEI	CTNAG	8 cut(s)	157, 207, 521, 790, 975, 1083, 1287, 1991
BstEII	GGTNACC	1 cut(s)	721
BstENI	CCTNNNNNAGG	2 cut(s)	271, 879
BstF5I	GGATG	6 cut(s)	1282, 1385, 1421, 1482, 1658, 1885
BstH2I	RGCGCY	2 cut(s)	157, 1499
BstHHI	GCGC	2 cut(s)	156, 1498
BstKTI	GATC	9 cut(s)	290, 856, 890, 1132, 1351, 1367, 2089, 2185, 2266
BstMAI	GTCTC	5 cut(s)	208, 797, 1068, 1762, 2243
BstMBI	GATC	9 cut(s)	287, 853, 887, 1129, 1348, 1364, 2086, 2182, 2263
BstMWI	GCNNNNNNNGC	8 cut(s)	40, 755, 1001, 1266, 1304, 1418, 1800, 1987
BstNI	CCWGG	1 cut(s)	542
BstPAI	GACNNNNGTC	1 cut(s)	1943
BstPI	GGTNACC	1 cut(s)	721
BstSCI	CCNGG	2 cut(s)	540, 1269
BstSFI	CTRYAG	3 cut(s)	169, 1229, 1302
BstSNI	TACGTA	1 cut(s)	282
BstV1I	GCAGC	4 cut(s)	1148, 1243, 1408, 1505
BstV2I	GAAGAC	2 cut(s)	132, 552
BstX2I	RGATCY	2 cut(s)	287, 1364
BstXI	CCANNNNNNTGG	1 cut(s)	338
BstYI	RGATCY	2 cut(s)	287, 1364
Bsu15I	ATCGAT	1 cut(s)	1132
Bsu36I	CCTNAGG	1 cut(s)	1287
BsuI	GTATCC	2 cut(s)	271, 655
BsuRI	GGCC	3 cut(s)	1341, 1768, 1822
BsuTUI	ATCGAT	1 cut(s)	1132
BtgZI	GCGATG	2 cut(s)	1743, 2247
BtsCI	GGATG	6 cut(s)	1282, 1385, 1421, 1482, 1658, 1885
BtsIMutI	CAGTG	6 cut(s)	426, 549, 936, 1261, 1959, 1984
BveI	ACCTGC	2 cut(s)	1439, 2116
Cac8I	GCNNGC	2 cut(s)	1159, 2230
CciI	TCATGA	2 cut(s)	799, 1600
CfoI	GCGC	2 cut(s)	156, 1498
Cfr13I	GGNCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
ClaI	ATCGAT	1 cut(s)	1132
Csp6I	GTAC	3 cut(s)	28, 62, 203
CspCI	CAANNNNNGTGG	2 cut(s)	987, 1022
CviQI	GTAC	3 cut(s)	28, 62, 203
DdeI	CTNAG	8 cut(s)	157, 207, 521, 790, 975, 1083, 1287, 1991
DpnI	GATC	9 cut(s)	289, 855, 889, 1131, 1350, 1366, 2088, 2184, 2265
DpnII	GATC	9 cut(s)	287, 853, 887, 1129, 1348, 1364, 2086, 2182, 2263
Eam1104I	CTCTTC	4 cut(s)	144, 298, 1090, 2241
EarI	CTCTTC	4 cut(s)	144, 298, 1090, 2241
Eco105I	TACGTA	1 cut(s)	282
Eco130I	CCWWGG	1 cut(s)	2111
Eco147I	AGGCCT	1 cut(s)	1822
Eco47I	GGWCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
Eco47III	AGCGCT	2 cut(s)	155, 1497
Eco57I	CTGAAG	3 cut(s)	1293, 1313, 2265
Eco81I	CCTNAGG	1 cut(s)	1287
Eco88I	CYCGRG	2 cut(s)	1666, 1759
Eco91I	GGTNACC	1 cut(s)	721
EcoNI	CCTNNNNNAGG	2 cut(s)	271, 879
EcoO109I	RGGNCCY	1 cut(s)	263
EcoO65I	GGTNACC	1 cut(s)	721
EcoRI	GAATTC	1 cut(s)	1478
EcoRII	CCWGG	1 cut(s)	540
EcoT14I	CCWWGG	1 cut(s)	2111
EcoT22I	ATGCAT	1 cut(s)	1551
ErhI	CCWWGG	1 cut(s)	2111
Esp3I	CGTCTC	1 cut(s)	1068
FalI	AAGNNNNNCTT	2 cut(s)	1707, 1739
FaqI	GGGAC	6 cut(s)	22, 81, 249, 1025, 1931, 2176
FauI	CCCGC	1 cut(s)	2191
FbaI	TGATCA	2 cut(s)	1348, 2086
FblI	GTMKAC	1 cut(s)	614
Fnu4HI	GCNGC	7 cut(s)	41, 756, 934, 1162, 1232, 1422, 1494
FokI	GGATG	6 cut(s)	1269, 1372, 1428, 1469, 1665, 1892
Fsp4HI	GCNGC	7 cut(s)	41, 756, 934, 1162, 1232, 1422, 1494
FspBI	CTAG	1 cut(s)	1029
GlaI	GCGC	2 cut(s)	155, 1497
GluI	GCNGC	7 cut(s)	41, 756, 934, 1162, 1232, 1422, 1494
GsuI	CTGGAG	1 cut(s)	200
HaeII	RGCGCY	2 cut(s)	157, 1499
HaeIII	GGCC	3 cut(s)	1341, 1768, 1822
HapII	CCGG	2 cut(s)	1271, 1816
HhaI	GCGC	2 cut(s)	156, 1498
Hin6I	GCGC	2 cut(s)	154, 1496
HinP1I	GCGC	2 cut(s)	154, 1496
HincII	GTYRAC	1 cut(s)	615
HindII	GTYRAC	1 cut(s)	615
HindIII	AAGCTT	2 cut(s)	1110, 1541
HinfI	GANTC	7 cut(s)	554, 593, 926, 967, 1013, 1022, 2096
HpaII	CCGG	2 cut(s)	1271, 1816
HphI	GGTGA	5 cut(s)	320, 602, 1285, 1763, 2062
Hpy166II	GTNNAC	3 cut(s)	615, 943, 2204
Hpy188III	TCNNGA	9 cut(s)	254, 800, 1241, 1286, 1453, 1601, 1759, 2093, 2154
Hpy8I	GTNNAC	3 cut(s)	615, 943, 2204
Hpy99I	CGWCG	1 cut(s)	2196
HpyAV	CCTTC	5 cut(s)	636, 643, 1459, 2037, 2172
HpyCH4III	ACNGT	6 cut(s)	868, 940, 1062, 1556, 2023, 2035
HpyCH4IV	ACGT	3 cut(s)	26, 281, 2214
HpyF10VI	GCNNNNNNNGC	8 cut(s)	40, 755, 1001, 1266, 1304, 1418, 1800, 1987
HpyF3I	CTNAG	8 cut(s)	157, 207, 521, 790, 975, 1083, 1287, 1991
HpySE526I	ACGT	3 cut(s)	26, 281, 2214
HspAI	GCGC	2 cut(s)	154, 1496
KflI	GGGWCCC	1 cut(s)	263
Ksp22I	TGATCA	2 cut(s)	1348, 2086
Kzo9I	GATC	9 cut(s)	287, 853, 887, 1129, 1348, 1364, 2086, 2182, 2263
LguI	GCTCTTC	1 cut(s)	144
LmnI	GCTCC	3 cut(s)	103, 1503, 2140
Lsp1109I	GCAGC	4 cut(s)	1148, 1243, 1408, 1505
LweI	GCATC	4 cut(s)	982, 1480, 1643, 1726
MaeI	CTAG	1 cut(s)	1029
MaeII	ACGT	3 cut(s)	26, 281, 2214
MaeIII	GTNAC	5 cut(s)	326, 721, 764, 1829, 2068
MalI	GATC	9 cut(s)	289, 855, 889, 1131, 1350, 1366, 2088, 2184, 2265
MboI	GATC	9 cut(s)	287, 853, 887, 1129, 1348, 1364, 2086, 2182, 2263
MfeI	CAATTG	4 cut(s)	218, 709, 1646, 2043
MflI	RGATCY	2 cut(s)	287, 1364
MlyI	GAGTC	2 cut(s)	976, 1007
MmeI	TCCRAC	2 cut(s)	475, 999
Mph1103I	ATGCAT	1 cut(s)	1551
MseI	TTAA	6 cut(s)	23, 198, 228, 858, 959, 1391
MslI	CAYNNNNRTG	3 cut(s)	431, 1554, 1878
MspA1I	CMGCKG	3 cut(s)	599, 1161, 1234
MspI	CCGG	2 cut(s)	1271, 1816
MspR9I	CCNGG	2 cut(s)	542, 1271
MunI	CAATTG	4 cut(s)	218, 709, 1646, 2043
Mva1269I	GAATGC	1 cut(s)	427
MvaI	CCWGG	1 cut(s)	542
MwoI	GCNNNNNNNGC	8 cut(s)	40, 755, 1001, 1266, 1304, 1418, 1800, 1987
NciI	CCSGG	1 cut(s)	1271
NdeII	GATC	9 cut(s)	287, 853, 887, 1129, 1348, 1364, 2086, 2182, 2263
NlaIV	GGNNCC	8 cut(s)	10, 264, 265, 289, 1156, 1383, 1671, 2109
NmeAIII	GCCGAG	1 cut(s)	237
NmuCI	GTSAC	3 cut(s)	326, 764, 2068
NsiI	ATGCAT	1 cut(s)	1551
PaeR7I	CTCGAG	1 cut(s)	1759
PagI	TCATGA	2 cut(s)	799, 1600
PceI	AGGCCT	1 cut(s)	1822
PciSI	GCTCTTC	1 cut(s)	144
PctI	GAATGC	1 cut(s)	427
PfeI	GAWTC	5 cut(s)	554, 593, 926, 1022, 2096
PflFI	GACNNNGTC	1 cut(s)	610
PkrI	GCNGC	7 cut(s)	42, 757, 935, 1163, 1233, 1423, 1495
PleI	GAGTC	2 cut(s)	975, 1007
PpsI	GAGTC	2 cut(s)	975, 1007
Ppu21I	YACGTR	1 cut(s)	282
PpuMI	RGGWCCY	1 cut(s)	263
PshAI	GACNNNNGTC	1 cut(s)	1943
PshBI	ATTAAT	1 cut(s)	228
Psp5II	RGGWCCY	1 cut(s)	263
Psp6I	CCWGG	1 cut(s)	540
PspEI	GGTNACC	1 cut(s)	721
PspGI	CCWGG	1 cut(s)	540
PspN4I	GGNNCC	8 cut(s)	10, 264, 265, 289, 1156, 1383, 1671, 2109
PspPI	GGNCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
PspPPI	RGGWCCY	1 cut(s)	263
PstI	CTGCAG	1 cut(s)	1233
PsuI	RGATCY	2 cut(s)	287, 1364
PsyI	GACNNNGTC	1 cut(s)	610
PvuII	CAGCTG	3 cut(s)	599, 1161, 1234
RsaI	GTAC	3 cut(s)	29, 63, 204
RsaNI	GTAC	3 cut(s)	28, 62, 203
RseI	CAYNNNNRTG	3 cut(s)	431, 1554, 1878
SalI	GTCGAC	1 cut(s)	613
SapI	GCTCTTC	1 cut(s)	144
SaqAI	TTAA	6 cut(s)	23, 198, 228, 858, 959, 1391
SatI	GCNGC	7 cut(s)	41, 756, 934, 1162, 1232, 1422, 1494
Sau3AI	GATC	9 cut(s)	287, 853, 887, 1129, 1348, 1364, 2086, 2182, 2263
Sau96I	GGNCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
SchI	GAGTC	2 cut(s)	976, 1007
ScrFI	CCNGG	2 cut(s)	542, 1271
SfaNI	GCATC	4 cut(s)	982, 1480, 1643, 1726
SfcI	CTRYAG	3 cut(s)	169, 1229, 1302
Sfr274I	CTCGAG	1 cut(s)	1759
SinI	GGWCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
SlaI	CTCGAG	1 cut(s)	1759
SmiMI	CAYNNNNRTG	3 cut(s)	431, 1554, 1878
SmlI	CTYRAG	4 cut(s)	834, 964, 1107, 1759
SmoI	CTYRAG	4 cut(s)	834, 964, 1107, 1759
SnaBI	TACGTA	1 cut(s)	282
SseBI	AGGCCT	1 cut(s)	1822
SsiI	CCGC	5 cut(s)	40, 408, 756, 933, 2198
SspMI	CTAG	1 cut(s)	1029
StuI	AGGCCT	1 cut(s)	1822
StyD4I	CCNGG	2 cut(s)	540, 1269
StyI	CCWWGG	1 cut(s)	2111
TaaI	ACNGT	6 cut(s)	868, 940, 1062, 1556, 2023, 2035
TaiI	ACGT	3 cut(s)	29, 284, 2217
TaqI	TCGA	4 cut(s)	614, 1132, 1760, 1937
TatI	WGTACW	1 cut(s)	61
TauI	GCSGC	3 cut(s)	43, 758, 936
TfiI	GAWTC	5 cut(s)	554, 593, 926, 1022, 2096
Tru1I	TTAA	6 cut(s)	23, 198, 228, 858, 959, 1391
Tru9I	TTAA	6 cut(s)	23, 198, 228, 858, 959, 1391
TscAI	CASTG	6 cut(s)	433, 556, 943, 1261, 1966, 1991
TseFI	GTSAC	3 cut(s)	326, 764, 2068
TseI	GCWGC	4 cut(s)	1161, 1231, 1421, 1493
Tsp45I	GTSAC	3 cut(s)	326, 764, 2068
TspDTI	ATGAA	9 cut(s)	128, 918, 1471, 1589, 1599, 1821, 1899, 2028, 2133
TspGWI	ACGGA	1 cut(s)	1330
TspRI	CASTG	6 cut(s)	433, 556, 943, 1261, 1966, 1991
Tth111I	GACNNNGTC	1 cut(s)	610
VpaK11BI	GGWCC	7 cut(s)	9, 263, 301, 904, 1382, 1670, 1964
VspI	ATTAAT	1 cut(s)	228
XagI	CCTNNNNNAGG	2 cut(s)	271, 879
XapI	RAATTY	4 cut(s)	239, 1478, 1886, 1975
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1721
XhoI	CTCGAG	1 cut(s)	1759
XmiI	GTMKAC	1 cut(s)	614
XspI	CTAG	1 cut(s)	1029
Zsp2I	ATGCAT	1 cut(s)	1551

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.