Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013257

Physical Location & Seq

Genomic Coordinates Reverse (-)

26884246 .. 26885494

Gene Structure 1249 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1177700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 912 bp

ATGCCCGCCGCCGCTCAGCTTCGCTGCTCTACAGTCGGCAGACACCACTACAACCCTTCCGACAACCGCAGATTCTCCGCCGACCCTAATTGTTCGATCTCGTTCCCCTACTCACAGACCCAGAATCGCACTCTCTCTTTCGTCCCAAAACCCATCTGGGTCCAAAAGCCAGAGTATTCGTTTTCCGGCGGAAATGGCGGTGGCGGAGCGGGAGCGGGAGGAAATAGTGGCGGATGGAGCCGAGGTGGAGATGGGAATTCCGACGACTCGTCGTCTTCGTCGTCGGTTTCGGGATTCGGAGTTCTGGGTATGTTTCTCAATGGGTGGAGATCCCGTGTCGCCGCCGATCCGCAGTTCCCCTTCAAGGTTCTGATGGAGGAGGTGGTCGGCGTCAGCTCCTGCGTCCTCGGCGACATGGCTTCCCGCCCCAATTTCGGCCTCAACGAGCTCGATTTCGTCTTCTCGACGCTCGTCGCACAGTCTTTTGCTGCCGTTGGATTCGTGGCGGGGCTTGCTGGAACCGCATTGTCGAATGGGCTGATCAAATTGAGGAAGAAGATGGATCCGAATTTCGAGACCCCGAATAAGGCGCCGCCTACGCTGCTGAATGCGCTCACTTGGGCGGCTCACATGGGTTTGAGCAGCAATTTGAGGTACCAGACGCTGAACGGGGCGGAGTTTTTGCTGGAGAAGGGGCTTCCGCCACTGGCGTTCAAGTCGTCTGTGGTGGTTCTGAGGTGCCTGAACAATGTGCTTGGCGGGATGTCGTTTGTTGTGCTGGCGAGGTTGACGGGGTCACAGAGCGTTGCGGAGAAACCGGTTGAAGTGGAGGTGGGATCGGCGGAGGAGAAGGAGAAATTGGTGGTGGTCGAGAGTGAGGATGAGCAGAGCAATCAGTCGACTTTCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000302 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0010154 GO:0022414 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0061458 GO:0099402 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RETICULATA-like

Protein Analysis

304

Amino Acids

32.14

Weight (kDa)

6.84

Isoelectric Point (pI)

43.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RETICULATA-like	PF11891	112 - 161	4.8e-15	Protein RETICULATA-related
RETICULATA-like	PF11891	161 - 224	8.5e-24	Protein RETICULATA-related

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08630 AT3G08640
fragaria_vesca	FvH4_6g09440
malus_domestica	MD04G1177700.v1.1 MD12G1193000.v1.1
prunus_persica	Prupe.6G297600_v2.0.a1
pyrus_communis	pycom04g15670
rosa_chinensis	RchiOBHm_Chr3g0459911
rosa_laevigata	RLG00000025052
rosa_multiflora	Rmu_co8396657.1_g000001 Rmu_sc0000240.1_g000046 Rmu_sc0016534.1_g000001
rosa_roxburghii	Rroxscaffold_6G00419920
rosa_rugosa	Rorug03G0038700.1
rosa_samantha	Rh3AG097500 Rh3BG100200 Rh3CG102000 Rh3DG101700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	654
AccB1I	GGYRCC	3 cut(s)	589, 654, 738
AccBSI	CCGCTC	3 cut(s)	14, 209, 215
AccI	GTMKAC	1 cut(s)	899
AclWI	GGATC	5 cut(s)	324, 341, 557, 570, 844
AcsI	RAATTY	2 cut(s)	256, 568
AcyI	GRCGYC	2 cut(s)	390, 590
AfaI	GTAC	1 cut(s)	656
AfiI	CCNNNNNNNGG	3 cut(s)	364, 434, 586
AgeI	ACCGGT	1 cut(s)	817
AgsI	TTSAA	4 cut(s)	364, 715, 824, 907
AhdI	GACNNNNNGTC	2 cut(s)	268, 271
AjuI	GAANNNNNNNTTGG	2 cut(s)	842, 874
AloI	GAACNNNNNNTCC	2 cut(s)	285, 317
AluBI	AGCT	3 cut(s)	19, 396, 448
AluI	AGCT	3 cut(s)	19, 396, 448
Alw21I	GWGCWC	1 cut(s)	450
Alw26I	GTCTC	1 cut(s)	569
AlwI	GGATC	5 cut(s)	324, 341, 557, 570, 844
AlwNI	CAGNNNCTG	2 cut(s)	399, 664
AoxI	GGCC	1 cut(s)	436
ApeKI	GCWGC	4 cut(s)	24, 488, 601, 642
ApoI	RAATTY	2 cut(s)	256, 568
AsiGI	ACCGGT	1 cut(s)	817
Asp718I	GGTACC	1 cut(s)	654
AspLEI	GCGC	2 cut(s)	592, 613
AspS9I	GGNCC	1 cut(s)	160
AvaII	GGWCC	1 cut(s)	160
BamHI	GGATCC	1 cut(s)	562
BanI	GGYRCC	3 cut(s)	589, 654, 738
BanII	GRGCYC	1 cut(s)	450
BbsI	GAAGAC	2 cut(s)	267, 451
Bbv12I	GWGCWC	1 cut(s)	450
BbvI	GCAGC	4 cut(s)	11, 475, 588, 654
BccI	CCATC	5 cut(s)	161, 228, 245, 367, 553
BceAI	ACGGC	1 cut(s)	476
BclI	TGATCA	1 cut(s)	540
BcoDI	GTCTC	1 cut(s)	569
BfmI	CTRYAG	1 cut(s)	30
BfoI	RGCGCY	1 cut(s)	593
BisI	GCNGC	9 cut(s)	9, 12, 25, 342, 489, 593, 602, 624, 643
BlpI	GCTNAGC	1 cut(s)	15
BlsI	GCNGC	9 cut(s)	10, 13, 26, 343, 490, 594, 603, 625, 644
Bme18I	GGWCC	1 cut(s)	160
BmeRI	GACNNNNNGTC	2 cut(s)	268, 271
BmgT120I	GGNCC	1 cut(s)	160
BmiI	GGNNCC	7 cut(s)	161, 239, 520, 564, 591, 656, 740
BoxI	GACNNNNGTC	1 cut(s)	470
BpiI	GAAGAC	2 cut(s)	267, 451
BpmI	CTGGAG	1 cut(s)	707
Bpu1102I	GCTNAGC	1 cut(s)	15
BsaHI	GRCGYC	2 cut(s)	390, 590
BsaI	GGTCTC	1 cut(s)	569
BsaJI	CCNNGG	2 cut(s)	241, 406
BsaWI	WCCGGW	1 cut(s)	817
BsaXI	ACNNNNNCTCC	4 cut(s)	229, 259, 319, 349
Bsc4I	CCNNNNNNNGG	3 cut(s)	364, 434, 586
Bse118I	RCCGGY	1 cut(s)	817
Bse1I	ACTGG	1 cut(s)	711
BseDI	CCNNGG	2 cut(s)	241, 406
BseGI	GGATG	3 cut(s)	239, 768, 886
BseLI	CCNNNNNNNGG	3 cut(s)	364, 434, 586
BseMII	CTCAG	2 cut(s)	29, 725
BseNI	ACTGG	1 cut(s)	711
BseRI	GAGGAG	2 cut(s)	392, 860
BseXI	GCAGC	4 cut(s)	11, 475, 588, 654
BshFI	GGCC	1 cut(s)	438
BshNI	GGYRCC	3 cut(s)	589, 654, 738
BshTI	ACCGGT	1 cut(s)	817
BsiHKAI	GWGCWC	1 cut(s)	450
BsiSI	CCGG	2 cut(s)	186, 818
BslFI	GGGAC	1 cut(s)	128
BslI	CCNNNNNNNGG	3 cut(s)	364, 434, 586
BsmAI	GTCTC	1 cut(s)	569
BsmFI	GGGAC	1 cut(s)	128
BsmI	GAATGC	1 cut(s)	613
BsnI	GGCC	1 cut(s)	438
Bso31I	GGTCTC	1 cut(s)	569
Bsp1286I	GDGCHC	1 cut(s)	450
Bsp143I	GATC	6 cut(s)	96, 329, 346, 540, 562, 836
Bsp1720I	GCTNAGC	1 cut(s)	15
BspANI	GGCC	1 cut(s)	438
BspCNI	CTCAG	2 cut(s)	28, 726
BspLI	GGNNCC	7 cut(s)	161, 239, 520, 564, 591, 656, 740
BspPI	GGATC	5 cut(s)	324, 341, 557, 570, 844
BspT107I	GGYRCC	3 cut(s)	589, 654, 738
BspTNI	GGTCTC	1 cut(s)	569
BsrBI	CCGCTC	3 cut(s)	14, 209, 215
BsrFI	RCCGGY	1 cut(s)	817
BsrI	ACTGG	1 cut(s)	711
BssAI	RCCGGY	1 cut(s)	817
BssECI	CCNNGG	2 cut(s)	241, 406
BssMI	GATC	6 cut(s)	96, 329, 346, 540, 562, 836
BssNI	GRCGYC	2 cut(s)	390, 590
Bst4CI	ACNGT	2 cut(s)	34, 480
BstACI	GRCGYC	2 cut(s)	390, 590
BstC8I	GCNNGC	3 cut(s)	6, 513, 780
BstDEI	CTNAG	2 cut(s)	15, 734
BstF5I	GGATG	3 cut(s)	239, 768, 886
BstH2I	RGCGCY	1 cut(s)	593
BstHHI	GCGC	2 cut(s)	592, 613
BstKTI	GATC	6 cut(s)	99, 332, 349, 543, 565, 839
BstMAI	GTCTC	1 cut(s)	569
BstMBI	GATC	6 cut(s)	96, 329, 346, 540, 562, 836
BstMWI	GCNNNNNNNGC	8 cut(s)	195, 237, 408, 512, 521, 598, 601, 610
BstPAI	GACNNNNGTC	1 cut(s)	470
BstSFI	CTRYAG	1 cut(s)	30
BstV1I	GCAGC	4 cut(s)	11, 475, 588, 654
BstV2I	GAAGAC	2 cut(s)	267, 451
BstX2I	RGATCY	2 cut(s)	329, 562
BstYI	RGATCY	2 cut(s)	329, 562
BsuRI	GGCC	1 cut(s)	438
BtsCI	GGATG	3 cut(s)	239, 768, 886
BtsIMutI	CAGTG	1 cut(s)	704
Cac8I	GCNNGC	3 cut(s)	6, 513, 780
CaiI	CAGNNNCTG	2 cut(s)	399, 664
CfoI	GCGC	2 cut(s)	592, 613
Cfr10I	RCCGGY	1 cut(s)	817
Cfr13I	GGNCC	1 cut(s)	160
CseI	GACGC	4 cut(s)	379, 391, 475, 670
Csp6I	GTAC	1 cut(s)	655
CspAI	ACCGGT	1 cut(s)	817
CviAII	CATG	2 cut(s)	415, 631
CviQI	GTAC	1 cut(s)	655
DdeI	CTNAG	2 cut(s)	15, 734
DinI	GGCGCC	1 cut(s)	591
DpnI	GATC	6 cut(s)	98, 331, 348, 542, 564, 838
DpnII	GATC	6 cut(s)	96, 329, 346, 540, 562, 836
DriI	GACNNNNNGTC	2 cut(s)	268, 271
Eam1105I	GACNNNNNGTC	2 cut(s)	268, 271
EciI	GGCGGA	7 cut(s)	67, 204, 219, 246, 689, 690, 857
Ecl136II	GAGCTC	1 cut(s)	448
Eco24I	GRGCYC	1 cut(s)	450
Eco31I	GGTCTC	1 cut(s)	569
Eco47I	GGWCC	1 cut(s)	160
Eco53kI	GAGCTC	1 cut(s)	448
EcoICRI	GAGCTC	1 cut(s)	448
EcoRI	GAATTC	1 cut(s)	256
EcoT38I	GRGCYC	1 cut(s)	450
EgeI	GGCGCC	1 cut(s)	591
EheI	GGCGCC	1 cut(s)	591
FaeI	CATG	2 cut(s)	418, 634
FaiI	YATR	3 cut(s)	311, 416, 632
FaqI	GGGAC	1 cut(s)	128
FatI	CATG	2 cut(s)	414, 630
FauI	CCCGC	6 cut(s)	13, 202, 208, 431, 499, 752
FbaI	TGATCA	1 cut(s)	540
FblI	GTMKAC	1 cut(s)	899
Fnu4HI	GCNGC	9 cut(s)	9, 12, 25, 342, 489, 593, 602, 624, 643
FokI	GGATG	3 cut(s)	246, 775, 893
FriOI	GRGCYC	1 cut(s)	450
Fsp4HI	GCNGC	9 cut(s)	9, 12, 25, 342, 489, 593, 602, 624, 643
GlaI	GCGC	2 cut(s)	591, 612
GluI	GCNGC	9 cut(s)	9, 12, 25, 342, 489, 593, 602, 624, 643
GsuI	CTGGAG	1 cut(s)	707
HaeII	RGCGCY	1 cut(s)	593
HaeIII	GGCC	1 cut(s)	438
HapII	CCGG	2 cut(s)	186, 818
HgaI	GACGC	4 cut(s)	379, 391, 475, 670
HhaI	GCGC	2 cut(s)	592, 613
Hin1I	GRCGYC	2 cut(s)	390, 590
Hin1II	CATG	2 cut(s)	418, 634
Hin6I	GCGC	2 cut(s)	590, 611
HinP1I	GCGC	2 cut(s)	590, 611
HincII	GTYRAC	2 cut(s)	789, 900
HindII	GTYRAC	2 cut(s)	789, 900
HinfI	GANTC	5 cut(s)	72, 124, 266, 294, 498
HpaII	CCGG	2 cut(s)	186, 818
Hpy166II	GTNNAC	2 cut(s)	789, 900
Hpy188I	TCNGA	6 cut(s)	61, 262, 299, 372, 567, 735
Hpy188III	TCNNGA	4 cut(s)	291, 463, 574, 871
Hpy8I	GTNNAC	2 cut(s)	789, 900
Hpy99I	CGWCG	6 cut(s)	266, 274, 283, 286, 469, 476
HpyAV	CCTTC	4 cut(s)	66, 370, 685, 844
HpyCH4III	ACNGT	2 cut(s)	34, 480
HpyF10VI	GCNNNNNNNGC	8 cut(s)	195, 237, 408, 512, 521, 598, 601, 610
HpyF3I	CTNAG	2 cut(s)	15, 734
Hsp92I	GRCGYC	2 cut(s)	390, 590
Hsp92II	CATG	2 cut(s)	418, 634
HspAI	GCGC	2 cut(s)	590, 611
KasI	GGCGCC	1 cut(s)	589
KpnI	GGTACC	1 cut(s)	658
Ksp22I	TGATCA	1 cut(s)	540
Kzo9I	GATC	6 cut(s)	96, 329, 346, 540, 562, 836
LmnI	GCTCC	4 cut(s)	206, 212, 237, 401
Lsp1109I	GCAGC	4 cut(s)	11, 475, 588, 654
MaeIII	GTNAC	1 cut(s)	795
MalI	GATC	6 cut(s)	98, 331, 348, 542, 564, 838
MbiI	CCGCTC	3 cut(s)	14, 209, 215
MboI	GATC	6 cut(s)	96, 329, 346, 540, 562, 836
MboII	GAAGA	4 cut(s)	267, 451, 565, 568
MflI	RGATCY	2 cut(s)	329, 562
MhlI	GDGCHC	1 cut(s)	450
MluCI	AATT	7 cut(s)	88, 256, 430, 545, 568, 646, 857
Mly113I	GGCGCC	1 cut(s)	590
MlyI	GAGTC	1 cut(s)	260
MmeI	TCCRAC	3 cut(s)	84, 285, 475
MspI	CCGG	2 cut(s)	186, 818
Mva1269I	GAATGC	1 cut(s)	613
MwoI	GCNNNNNNNGC	8 cut(s)	195, 237, 408, 512, 521, 598, 601, 610
NarI	GGCGCC	1 cut(s)	590
NdeII	GATC	6 cut(s)	96, 329, 346, 540, 562, 836
NlaIII	CATG	2 cut(s)	418, 634
NlaIV	GGNNCC	7 cut(s)	161, 239, 520, 564, 591, 656, 740
NmeAIII	GCCGAG	2 cut(s)	266, 387
NmuCI	GTSAC	1 cut(s)	795
PcsI	WCGNNNNNNNCGW	3 cut(s)	275, 278, 441
PctI	GAATGC	1 cut(s)	613
PfeI	GAWTC	4 cut(s)	72, 124, 294, 498
PflFI	GACNNNGTC	1 cut(s)	793
PinAI	ACCGGT	1 cut(s)	817
PkrI	GCNGC	9 cut(s)	10, 13, 26, 343, 490, 594, 603, 625, 644
PleI	GAGTC	1 cut(s)	260
PluTI	GGCGCC	1 cut(s)	593
PpsI	GAGTC	1 cut(s)	260
PshAI	GACNNNNGTC	1 cut(s)	470
Psp124BI	GAGCTC	1 cut(s)	450
PspN4I	GGNNCC	7 cut(s)	161, 239, 520, 564, 591, 656, 740
PspPI	GGNCC	1 cut(s)	160
PstNI	CAGNNNCTG	2 cut(s)	399, 664
PsuI	RGATCY	2 cut(s)	329, 562
PsyI	GACNNNGTC	1 cut(s)	793
RsaI	GTAC	1 cut(s)	656
RsaNI	GTAC	1 cut(s)	655
SacI	GAGCTC	1 cut(s)	450
SalI	GTCGAC	1 cut(s)	898
SatI	GCNGC	9 cut(s)	9, 12, 25, 342, 489, 593, 602, 624, 643
Sau3AI	GATC	6 cut(s)	96, 329, 346, 540, 562, 836
Sau96I	GGNCC	1 cut(s)	160
SchI	GAGTC	1 cut(s)	260
SduI	GDGCHC	1 cut(s)	450
SfcI	CTRYAG	1 cut(s)	30
SfoI	GGCGCC	1 cut(s)	591
SinI	GGWCC	1 cut(s)	160
Sse9I	AATT	7 cut(s)	88, 256, 430, 545, 568, 646, 857
SspDI	GGCGCC	1 cut(s)	589
SstI	GAGCTC	1 cut(s)	450
TaaI	ACNGT	2 cut(s)	34, 480
TaqI	TCGA	7 cut(s)	95, 450, 464, 530, 573, 870, 899
TasI	AATT	7 cut(s)	88, 256, 430, 545, 568, 646, 857
TauI	GCSGC	5 cut(s)	11, 14, 344, 595, 626
TfiI	GAWTC	4 cut(s)	72, 124, 294, 498
TscAI	CASTG	1 cut(s)	711
TseFI	GTSAC	1 cut(s)	795
TseI	GCWGC	4 cut(s)	24, 488, 601, 642
Tsp45I	GTSAC	1 cut(s)	795
TspRI	CASTG	1 cut(s)	711
Tth111I	GACNNNGTC	1 cut(s)	793
VpaK11BI	GGWCC	1 cut(s)	160
XapI	RAATTY	2 cut(s)	256, 568
XcmI	CCANNNNNNNNNTGG	1 cut(s)	153
XmiI	GTMKAC	1 cut(s)	899

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.