Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004774

Physical Location & Seq

Genomic Coordinates Forward (+)

27941759 .. 27950838

Gene Structure 9080 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1188900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3378 bp

ATGGGGACTGAAAACCCTGGTCGTCCGAATTTTACTACAAGACCTGCCACTGCACCATTTGCGGCTCCTCCTCAGAACATGATGCCTTTTTCATCTTCAGGTCCCATGGTTGGACAGGAGGCATCTGGTTTTAGACCTCCTCCACATGTCACTCAACAGACACCTTTTTCATCGTCTGGGCCTGTGGTTGGTGCAGCTGCCTCTAACTTCGGACCTACTCCACCGGTTGCTCCACAGACTAATGTGCCGTTCTCGTCAACTGGATCTGCAGTTGGAGCACAAGCCTCCCATTTTAGACCTACCCCGCCAGGTAGGTTTCATGATCCATCTGTGCCTCCTCCGCCAACTTCAAGTGTCCCGCCGAGTGTTGGACCTTTGTCCCGTTTCCCAACACCACAATATCTATCAGCAGCACAAGCACCCCCTACACGTGGTCCACCTGTAGGCCAAATGCCATATCAACCTCCTCCAGGTCAAGCACCATTTCAACGACCACAACAGCAGATACCTTCTGTGCCAATGGGACCTCCACCTCAAAGCTTTAATTCTGTACCACCAAGTGTGAATGCTCTTCAGTCTCCATCAGATTCATCATTCCCAGCTTCCCTACCAAATTCTCAGACATCTTTTCCTGGATTCCCCCGTAAGCAGTCTAGTGCAGACTCACAAGCCCCACCTGCTCAGTCTCCATTTCTTACTCATCAAGGAAACTATGCAGCTGCTCCACCAGCAGTGTCCTCTCCTTTTGCTTCTCACCAAGGAGGTTATGCTCCACCTCCGCAGGGTGCAGCTCCATTGGGTATGCAGTCAAGGGACCACATGCAACATCACGGTTCTGGACCTCCTGTTGGTGCTGTCCAAGCCTTGACGGACGACTTCAGCTCACTATCTATTGGATCTGTTCCCGGATCAATTGAACCTGGACTTGACCCTAAAGCTTTGCCAAGGCCTTTGGCTGGTGACGTGGAGCCAAAAGCACTTGCTCAGATGTATCCCATGAACTGCAATCCTAGATTTCTACGGTTTACAACTGGTGCAATACCAAGTTCTCAGTCTTTGTCTTCAAGGTGGCATTTACCTTTGGGAGCAGTTGTTTGTCCACTTGCTGAACCACCTGATGGGGAGGAAGTTCCAATTGTTAATTTTGGTTCTGCCGGGATTATTCGCTGTAGAAGATGTCGGACATATGTGAACCCCTATGTGACATTTACAGATGCTGGAAGAAAGTGGCGTTGCAACATTTGTGCTCTGCTTAATGATGTCCCTGGGGATTACTTTGCTCATTTGGATGCCACTGGCAGAAGAATTGATTTGGATCAACGACCTGAGCTTACATGGGGTAGCGTGGAATTTGTTGCTCCAACTGAATATATGGTGCGGCCTCCTATGCCGCCACTGTATTTTTTCCTCATTGACGTTTCGTTATCTGCTGTCAGAAGTGGTATGATTGAGGTTGTGGCCCAAACTATCCGGTCATGCTTGGATGAGCTTCCTGGCTTCCCCAGAACACAAATTGGGTTTGCAACTTTTGACAGCACAATACATTTTTATAACTTGAAATCTTCTTTGACTCAGCCTCAAATGATGGTGGTCTCAGACCTGGATGATGTATTTGTTCCATTGCCAGATGATCTTCTTGTTAATCTATCTGAATCAAGAAGTGTAGTGGAGACCTTCCTAGATAGCTTGCCGTCTATGTTTCAAGACAATGTGAATGTGGAGTCTGCTTTTGGTCCAGCTCTTAAAGCCTCACTTATGCTTATGAGCAACCTTGGGGGAAAATTGTTAATTTTTCAAAATACACTGCCATCTCTTGGTGTTGGTCGCTTGAAGTTGCGTGGAGATGATCTTCGTGTCTATGGCTCTGATAAAGAGCATTCACTAAGACTACCAGAGGATCCTTTCTACAAGCAGATGGCTGCAGAATTTACCAAGTTCCAGATAGGGGTGGATGTGTATGCCTTCAGTGACAATTACACTGATATAGCTTCATTAGGTACTCTGGCAAAATATACTGGGGGTCAGGTGTATTATTACCCGAATTTTCTATCAACCATTCATGGAGATAAGTTGAGACATGAATTAGCCAGAGATCTTACTAGAGAAACTGCCTGGGAAGCAGTGATGCGCATAAGATGTGGAAAAGGAGTGCGCTTTACGTCTTACCATGGGAACTTTATGCTAAGGTCCACAGACTTATTAGCACTTCCTGCTGTAGATTGTGATAAAGCATTTGCAATGCAGTTATCTCTTGAAGAGACGCTTCTAACTATTCAGACAGTATATTTCCAAGTTGCCTTGCTATACACTGCATCCTGCGGAGAGAGGCGTATTAGAGTACATACAGCAGCAGTACCGGTGGTTACAGATCTGGCTGAGATGTATCGGCAGGCTGATACTGGTGCTATTGTCACCTTGCTTTCCAGGCTAGCAATTGAGAAAACTTTGTCCCATAAGTTGGAAGATGCTCGGAACTCTCTGCAGCTAAGAATTGTCAAAGCTCTCAAAGAATTTCGAAATCTCTATGCTGTACAACACCGCTTAGGAGGGAAGATGATATACTCAGAGTCTCTGAAGTTCTTGCCTTTGTATGGATTGGCACTTTGTAAATCAGCACCTCTTCGTGGGGGGTATGCAGACATGTCACTTGATGAGCGTTGTGCGGCAGGCCACACAATGATGACCTTACCAGTTAAAAAGTTGTTGAAGCTCCTGTATCCCAGTTTAATACGGCTCGATGAGTATCTTTTGAACGCATCTTCTGAAGCTGATGACGTCAAAATCATAGAGAATAGGTTACCACTACTTGCCGAGAGCTTAGATTTCAGAGGCCTTTATGTATTCGATGATGGGTTTCGCCATGTTCTATGGTTTGGTAGAGTTCTTCCACCTGATATAGCTAAGAATCTACTTGGGCCTGACTTTGCAGCAGAGTTATCAAAGGTTACCCTTTGTGAGCGAGATAATCAAATGTCAAAGAAGCTAATGAGGATACTAAAGAAATTCAGAGAAAGTGATCCTTCACATTATCAGTTGTGTTATCTTGTAAGACAAGGTGAGCAGCCCCGAGAAGGCCATCTTATACTTGCCAATCTTGTTGAGGAGCAGATGGGCGGAAGCAATGGTTACGTTGATTGGATAATACAAGTGCACCGGCAAGTCCAGCAAAATCCGACGTTTATTAAGCAGACTAGATGCCTCATCTGTAGAGTGGTTGAGCATTATTTACCTGGAAAAAAAGAAGAAGTAATTGAGCATTATTTTGAATTCAGCCATGGGAGCACCCCATGCTGTAGAGTCGAGGATGTTGAAGAACGGGTGGCTACCAGGCACAGATTTTTGGACGATTCGGTACGTCATCATCACAGGACGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0006275 GO:0006996 GO:0007029 GO:0007030 GO:0007276 GO:0008150 GO:0008156 GO:0008361 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010256 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032504 GO:0032535 GO:0032875 GO:0032876 GO:0044703 GO:0045786 GO:0045934 GO:0048232 GO:0048519 GO:0048523 GO:0048609 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051704 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0080119 GO:0090066 GO:0090329 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

1126

Amino Acids

123.87

Weight (kDa)

7.01

Isoelectric Point (pI)

52.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-Sec23_Sec24	PF04810	387 - 425	1.3e-17	Sec23/Sec24 zinc finger
Sec23_trunk	PF04811	462 - 698	5.3e-82	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	704 - 788	8.8e-25	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	799 - 902	1.9e-23	Sec23/Sec24 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07100
fragaria_vesca	FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220
malus_domestica	MD04G1188900.v1.1 MD12G1201700.v1.1
prunus_persica	Prupe.6G306800_v2.0.a1 Prupe.6G306800_v2.0.a1 Prupe.6G306800_v2.0.a1
pyrus_communis	pycom04g16720 pycom04g16730 pycom12g18800 pycom12g18810
rosa_chinensis	RchiOBHm_Chr3g0458141
rosa_laevigata	RLG00000025175
rosa_multiflora	Rmu_sc0019452.1_g000001
rosa_roxburghii	Rroxscaffold_6G00421270
rosa_rugosa	Rorug03G0024700 Rorug03G0024800 Rorug03G0024900 Rorug03G0024900 Rorug03G0025000
rosa_samantha	Rh3AG085700 Rh3BG088400 Rh3CG089000 Rh3DG089400
rosa_wichuraiana	Rw3G007280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1551
AarI	CACCTGC	1 cut(s)	685
AatII	GACGTC	1 cut(s)	2778
Acc16I	TGCGCA	1 cut(s)	2129
Acc36I	ACCTGC	2 cut(s)	52, 685
AccB7I	CCANNNNNTGG	3 cut(s)	1118, 1814, 2458
AclWI	GGATC	8 cut(s)	271, 317, 904, 916, 1323, 1892, 1905, 3011
AcsI	RAATTY	8 cut(s)	28, 613, 1349, 1925, 2041, 2510, 3002, 3266
AcuI	CTGAAG	6 cut(s)	81, 557, 862, 1948, 2594, 2784
AcvI	CACGTG	1 cut(s)	431
AcyI	GRCGYC	1 cut(s)	2775
AdeI	CACNNNGTG	1 cut(s)	560
AfaI	GTAC	6 cut(s)	552, 1999, 2340, 2355, 2532, 3354
AflIII	ACRYGT	3 cut(s)	145, 428, 2640
AgeI	ACCGGT	2 cut(s)	223, 2356
AjiI	CACGTC	1 cut(s)	964
Alw21I	GWGCWC	4 cut(s)	280, 1249, 3153, 3284
Alw26I	GTCTC	7 cut(s)	582, 690, 1597, 1664, 2068, 2252, 2574
Alw44I	GTGCAC	1 cut(s)	3149
AlwI	GGATC	8 cut(s)	271, 317, 904, 916, 1323, 1892, 1905, 3011
AlwNI	CAGNNNCTG	1 cut(s)	1217
Ama87I	CYCGRG	1 cut(s)	3066
AoxI	GGCC	9 cut(s)	179, 445, 947, 1379, 1458, 2668, 2830, 2915, 3073
ApaLI	GTGCAC	1 cut(s)	3149
ApoI	RAATTY	8 cut(s)	28, 613, 1349, 1925, 2041, 2510, 3002, 3266
AsiGI	ACCGGT	2 cut(s)	223, 2356
AspLEI	GCGC	2 cut(s)	2130, 2154
AsuC2I	CCSGG	2 cut(s)	906, 1156
AsuHPI	GGTGA	4 cut(s)	746, 971, 2404, 3068
AsuII	TTCGAA	1 cut(s)	2515
AsuNHI	GCTAGC	1 cut(s)	2428
AvaI	CYCGRG	1 cut(s)	3066
AvaII	GGWCC	9 cut(s)	101, 212, 371, 434, 524, 814, 839, 1733, 2187
BaeGI	GKGCMC	1 cut(s)	3153
BamHI	GGATCC	1 cut(s)	1897
BarI	GAAGNNNNNNTAC	2 cut(s)	2543, 2575
BbrPI	CACGTG	1 cut(s)	431
BbsI	GAAGAC	1 cut(s)	1053
Bbv12I	GWGCWC	4 cut(s)	280, 1249, 3153, 3284
BccI	CCATC	9 cut(s)	334, 589, 1112, 1579, 1816, 1909, 2843, 3084, 3103
BceAI	ACGGC	3 cut(s)	232, 1675, 2747
BciVI	GTATCC	3 cut(s)	1002, 2727, 2985
BcnI	CCSGG	2 cut(s)	906, 1156
BcoDI	GTCTC	7 cut(s)	582, 690, 1597, 1664, 2068, 2252, 2574
BfaI	CTAG	6 cut(s)	654, 1011, 1679, 2100, 2429, 3192
BfmI	CTRYAG	8 cut(s)	267, 441, 1168, 1920, 2214, 2480, 3205, 3292
BfuAI	ACCTGC	2 cut(s)	52, 685
BfuI	GTATCC	3 cut(s)	1002, 2727, 2985
BglII	AGATCT	2 cut(s)	2092, 2368
Bme18I	GGWCC	9 cut(s)	101, 212, 371, 434, 524, 814, 839, 1733, 2187
BmeT110I	CYCGRG	1 cut(s)	3066
BmgBI	CACGTC	1 cut(s)	964
BmiI	GGNNCC	6 cut(s)	66, 103, 525, 815, 969, 1899
BmrI	ACTGGG	2 cut(s)	2025, 2715
BmsI	GCATC	9 cut(s)	72, 131, 1204, 1279, 2115, 2321, 2455, 2765, 3185
BmtI	GCTAGC	1 cut(s)	2432
BmuI	ACTGGG	2 cut(s)	2025, 2715
BoxI	GACNNNNGTC	1 cut(s)	376
BpiI	GAAGAC	1 cut(s)	1053
BpmI	CTGGAG	1 cut(s)	453
Bpu10I	CCTNAGC	3 cut(s)	1326, 2183, 2542
Bpu14I	TTCGAA	1 cut(s)	2515
BpuMI	CCSGG	2 cut(s)	906, 1156
BsaAI	YACGTR	1 cut(s)	431
BsaBI	GATNNNNATC	2 cut(s)	1314, 2742
BsaHI	GRCGYC	1 cut(s)	2775
BsaI	GGTCTC	2 cut(s)	1597, 1664
BsaWI	WCCGGW	3 cut(s)	223, 1470, 2356
BsaXI	ACNNNNNCTCC	2 cut(s)	1077, 1107
Bse118I	RCCGGY	3 cut(s)	223, 2356, 3153
Bse1I	ACTGG	7 cut(s)	265, 1036, 1300, 2020, 2404, 2690, 2721
Bse3DI	GCAATG	3 cut(s)	1619, 2244, 3127
Bse8I	GATNNNNATC	2 cut(s)	1314, 2742
BseGI	GGATG	6 cut(s)	1294, 1489, 1609, 1957, 2312, 3310
BseJI	GATNNNNATC	2 cut(s)	1314, 2742
BseMI	GCAATG	3 cut(s)	1619, 2244, 3127
BseNI	ACTGG	7 cut(s)	265, 1036, 1300, 2020, 2404, 2690, 2721
BseRI	GAGGAG	6 cut(s)	57, 60, 129, 327, 456, 3116
BseSI	GKGCMC	1 cut(s)	3153
BseYI	CCCAGC	1 cut(s)	598
BsgI	GTGCAG	4 cut(s)	36, 213, 678, 807
BshFI	GGCC	9 cut(s)	181, 447, 949, 1381, 1460, 2670, 2832, 2917, 3075
BshTI	ACCGGT	2 cut(s)	223, 2356
BsiHKAI	GWGCWC	4 cut(s)	280, 1249, 3153, 3284
BsiHKCI	CYCGRG	1 cut(s)	3066
BsiSI	CCGG	6 cut(s)	224, 906, 1155, 1471, 2357, 3154
BslFI	GGGAC	8 cut(s)	19, 87, 341, 364, 537, 827, 1247, 2434
BsmAI	GTCTC	7 cut(s)	582, 690, 1597, 1664, 2068, 2252, 2574
BsmBI	CGTCTC	1 cut(s)	2252
BsmFI	GGGAC	8 cut(s)	19, 87, 341, 364, 537, 827, 1247, 2434
BsmI	GAATGC	2 cut(s)	571, 1876
BsnI	GGCC	9 cut(s)	181, 447, 949, 1381, 1460, 2670, 2832, 2917, 3075
Bso31I	GGTCTC	2 cut(s)	1597, 1664
BsoBI	CYCGRG	1 cut(s)	3066
Bsp119I	TTCGAA	1 cut(s)	2515
Bsp1286I	GDGCHC	4 cut(s)	280, 1249, 3153, 3284
Bsp1407I	TGTACA	1 cut(s)	2530
Bsp19I	CCATGG	3 cut(s)	105, 2167, 3274
BspANI	GGCC	9 cut(s)	181, 447, 949, 1381, 1460, 2670, 2832, 2917, 3075
BspHI	TCATGA	1 cut(s)	319
BspLI	GGNNCC	6 cut(s)	66, 103, 525, 815, 969, 1899
BspMAI	CTGCAG	3 cut(s)	271, 1924, 2484
BspMI	ACCTGC	2 cut(s)	52, 685
BspOI	GCTAGC	1 cut(s)	2432
BspPI	GGATC	8 cut(s)	271, 317, 904, 916, 1323, 1892, 1905, 3011
BspQI	GCTCTTC	1 cut(s)	576
BspT104I	TTCGAA	1 cut(s)	2515
BspTNI	GGTCTC	2 cut(s)	1597, 1664
BsrDI	GCAATG	3 cut(s)	1619, 2244, 3127
BsrFI	RCCGGY	3 cut(s)	223, 2356, 3153
BsrGI	TGTACA	1 cut(s)	2530
BsrI	ACTGG	7 cut(s)	265, 1036, 1300, 2020, 2404, 2690, 2721
BssAI	RCCGGY	3 cut(s)	223, 2356, 3153
BssNI	GRCGYC	1 cut(s)	2775
BssT1I	CCWWGG	6 cut(s)	105, 757, 944, 1771, 2167, 3274
Bst4CI	ACNGT	4 cut(s)	833, 1023, 1398, 2281
Bst6I	CTCTTC	3 cut(s)	576, 2250, 2625
BstACI	GRCGYC	1 cut(s)	2775
BstAPI	GCANNNNNTGC	3 cut(s)	59, 2210, 3288
BstAUI	TGTACA	1 cut(s)	2530
BstBAI	YACGTR	1 cut(s)	431
BstBI	TTCGAA	1 cut(s)	2515
BstC8I	GCNNGC	4 cut(s)	1688, 2391, 2430, 2668
BstDSI	CCRYGG	3 cut(s)	105, 2167, 3274
BstEII	GGTNACC	2 cut(s)	2796, 2944
BstENI	CCTNNNNNAGG	1 cut(s)	468
BstF5I	GGATG	6 cut(s)	1294, 1489, 1609, 1957, 2312, 3310
BstHHI	GCGC	2 cut(s)	2130, 2154
BstMAI	GTCTC	7 cut(s)	582, 690, 1597, 1664, 2068, 2252, 2574
BstNSI	RCATGY	3 cut(s)	149, 823, 2644
BstPAI	GACNNNNGTC	1 cut(s)	376
BstPI	GGTNACC	2 cut(s)	2796, 2944
BstSFI	CTRYAG	8 cut(s)	267, 441, 1168, 1920, 2214, 2480, 3205, 3292
BstSLI	GKGCMC	1 cut(s)	3153
BstV2I	GAAGAC	1 cut(s)	1053
BstX2I	RGATCY	5 cut(s)	263, 896, 1897, 2092, 2368
BstYI	RGATCY	5 cut(s)	263, 896, 1897, 2092, 2368
BsuI	GTATCC	3 cut(s)	1002, 2727, 2985
BsuRI	GGCC	9 cut(s)	181, 447, 949, 1381, 1460, 2670, 2832, 2917, 3075
BtgI	CCRYGG	3 cut(s)	105, 2167, 3274
BtrI	CACGTC	1 cut(s)	964
BtsCI	GGATG	6 cut(s)	1294, 1489, 1609, 1957, 2312, 3310
BtsI	GCAGTG	5 cut(s)	48, 738, 1802, 2127, 2307
BtsIMutI	CAGTG	9 cut(s)	48, 738, 1293, 1394, 1802, 1972, 1977, 2127, 2307
BveI	ACCTGC	2 cut(s)	52, 685
Cac8I	GCNNGC	4 cut(s)	1688, 2391, 2430, 2668
CaiI	CAGNNNCTG	1 cut(s)	1217
CciI	TCATGA	1 cut(s)	319
CfoI	GCGC	2 cut(s)	2130, 2154
Cfr10I	RCCGGY	3 cut(s)	223, 2356, 3153
CseI	GACGC	1 cut(s)	2269
Csp6I	GTAC	6 cut(s)	551, 1998, 2339, 2354, 2531, 3353
CspAI	ACCGGT	2 cut(s)	223, 2356
CviQI	GTAC	6 cut(s)	551, 1998, 2339, 2354, 2531, 3353
DraIII	CACNNNGTG	1 cut(s)	560
Eam1104I	CTCTTC	3 cut(s)	576, 2250, 2625
EarI	CTCTTC	3 cut(s)	576, 2250, 2625
EciI	GGCGGA	2 cut(s)	330, 3129
Eco130I	CCWWGG	6 cut(s)	105, 757, 944, 1771, 2167, 3274
Eco147I	AGGCCT	2 cut(s)	949, 2832
Eco31I	GGTCTC	2 cut(s)	1597, 1664
Eco47I	GGWCC	9 cut(s)	101, 212, 371, 434, 524, 814, 839, 1733, 2187
Eco57I	CTGAAG	6 cut(s)	81, 557, 862, 1948, 2594, 2784
Eco72I	CACGTG	1 cut(s)	431
Eco88I	CYCGRG	1 cut(s)	3066
Eco91I	GGTNACC	2 cut(s)	2796, 2944
EcoNI	CCTNNNNNAGG	1 cut(s)	468
EcoO109I	RGGNCCY	2 cut(s)	101, 524
EcoO65I	GGTNACC	2 cut(s)	2796, 2944
EcoRI	GAATTC	1 cut(s)	3266
EcoT14I	CCWWGG	6 cut(s)	105, 757, 944, 1771, 2167, 3274
ErhI	CCWWGG	6 cut(s)	105, 757, 944, 1771, 2167, 3274
Esp3I	CGTCTC	1 cut(s)	2252
FalI	AAGNNNNNCTT	8 cut(s)	1737, 1769, 2247, 2279, 3004, 3036, 3063, 3095
FaqI	GGGAC	8 cut(s)	19, 87, 341, 364, 537, 827, 1247, 2434
FauI	CCCGC	2 cut(s)	312, 366
FauNDI	CATATG	1 cut(s)	1186
FokI	GGATG	6 cut(s)	1301, 1496, 1616, 1964, 2299, 3317
FspAI	RTGCGCAY	1 cut(s)	2129
FspBI	CTAG	6 cut(s)	654, 1011, 1679, 2100, 2429, 3192
FspI	TGCGCA	1 cut(s)	2129
GlaI	GCGC	2 cut(s)	2129, 2153
GsaI	CCCAGC	1 cut(s)	602
GsuI	CTGGAG	1 cut(s)	453
HaeIII	GGCC	9 cut(s)	181, 447, 949, 1381, 1460, 2670, 2832, 2917, 3075
HapII	CCGG	6 cut(s)	224, 906, 1155, 1471, 2357, 3154
HgaI	GACGC	1 cut(s)	2269
HhaI	GCGC	2 cut(s)	2130, 2154
Hin1I	GRCGYC	1 cut(s)	2775
Hin6I	GCGC	2 cut(s)	2128, 2152
HinP1I	GCGC	2 cut(s)	2128, 2152
HincII	GTYRAC	1 cut(s)	258
HindII	GTYRAC	1 cut(s)	258
HindIII	AAGCTT	2 cut(s)	538, 936
HpaII	CCGG	6 cut(s)	224, 906, 1155, 1471, 2357, 3154
HphI	GGTGA	4 cut(s)	746, 971, 2404, 3068
Hpy166II	GTNNAC	7 cut(s)	258, 437, 1026, 1100, 1192, 2190, 3151
Hpy188III	TCNNGA	6 cut(s)	320, 837, 1656, 1703, 1939, 2252
Hpy8I	GTNNAC	7 cut(s)	258, 437, 1026, 1100, 1192, 2190, 3151
Hpy99I	CGWCG	1 cut(s)	3178
HpyAV	CCTTC	5 cut(s)	519, 1684, 1972, 3030, 3065
HpyCH4III	ACNGT	4 cut(s)	833, 1023, 1398, 2281
HpyCH4IV	ACGT	8 cut(s)	430, 963, 1416, 2159, 2775, 3129, 3176, 3355
HpySE526I	ACGT	8 cut(s)	430, 963, 1416, 2159, 2775, 3129, 3176, 3355
Hsp92I	GRCGYC	1 cut(s)	2775
HspAI	GCGC	2 cut(s)	2128, 2152
LguI	GCTCTTC	1 cut(s)	576
LweI	GCATC	9 cut(s)	72, 131, 1204, 1279, 2115, 2321, 2455, 2765, 3185
MaeI	CTAG	6 cut(s)	654, 1011, 1679, 2100, 2429, 3192
MaeII	ACGT	8 cut(s)	430, 963, 1416, 2159, 2775, 3129, 3176, 3355
MfeI	CAATTG	3 cut(s)	912, 1134, 2433
MflI	RGATCY	5 cut(s)	263, 896, 1897, 2092, 2368
MhlI	GDGCHC	4 cut(s)	280, 1249, 3153, 3284
MlyI	GAGTC	5 cut(s)	656, 1564, 1730, 2576, 3306
MmeI	TCCRAC	7 cut(s)	91, 253, 349, 1160, 1385, 2439, 3197
MseI	TTAA	9 cut(s)	543, 1140, 1254, 1641, 1743, 1787, 2694, 2726, 3183
MslI	CAYNNNNRTG	2 cut(s)	1287, 2678
MspA1I	CMGCKG	2 cut(s)	197, 719
MspI	CCGG	6 cut(s)	224, 906, 1155, 1471, 2357, 3154
MunI	CAATTG	3 cut(s)	912, 1134, 2433
Mva1269I	GAATGC	2 cut(s)	571, 1876
NciI	CCSGG	2 cut(s)	906, 1156
NcoI	CCATGG	3 cut(s)	105, 2167, 3274
NdeI	CATATG	1 cut(s)	1186
NheI	GCTAGC	1 cut(s)	2428
NlaIV	GGNNCC	6 cut(s)	66, 103, 525, 815, 969, 1899
NmeAIII	GCCGAG	2 cut(s)	387, 2836
NmuCI	GTSAC	6 cut(s)	148, 959, 1201, 1967, 2410, 2643
NsbI	TGCGCA	1 cut(s)	2129
NspI	RCATGY	3 cut(s)	149, 823, 2644
NspV	TTCGAA	1 cut(s)	2515
PagI	TCATGA	1 cut(s)	319
PaqCI	CACCTGC	1 cut(s)	685
PasI	CCCWGGG	1 cut(s)	1265
PceI	AGGCCT	2 cut(s)	949, 2832
PciI	ACATGT	2 cut(s)	145, 2640
PciSI	GCTCTTC	1 cut(s)	576
PctI	GAATGC	2 cut(s)	571, 1876
PfeI	GAWTC	5 cut(s)	587, 636, 1652, 2905, 3347
PflMI	CCANNNNNTGG	3 cut(s)	1118, 1814, 2458
PfoI	TCCNGGA	2 cut(s)	631, 904
PinAI	ACCGGT	2 cut(s)	223, 2356
PleI	GAGTC	5 cut(s)	656, 1564, 1729, 2575, 3305
PmaCI	CACGTG	1 cut(s)	431
PmlI	CACGTG	1 cut(s)	431
PpsI	GAGTC	5 cut(s)	656, 1564, 1729, 2575, 3305
Ppu21I	YACGTR	1 cut(s)	431
PpuMI	RGGWCCY	2 cut(s)	101, 524
PscI	ACATGT	2 cut(s)	145, 2640
PshAI	GACNNNNGTC	1 cut(s)	376
PsiI	TTATAA	1 cut(s)	1551
Psp5II	RGGWCCY	2 cut(s)	101, 524
PspCI	CACGTG	1 cut(s)	431
PspEI	GGTNACC	2 cut(s)	2796, 2944
PspFI	CCCAGC	1 cut(s)	598
PspN4I	GGNNCC	6 cut(s)	66, 103, 525, 815, 969, 1899
PspPPI	RGGWCCY	2 cut(s)	101, 524
PstI	CTGCAG	3 cut(s)	271, 1924, 2484
PstNI	CAGNNNCTG	1 cut(s)	1217
PsuI	RGATCY	5 cut(s)	263, 896, 1897, 2092, 2368
PvuII	CAGCTG	2 cut(s)	197, 719
RsaI	GTAC	6 cut(s)	552, 1999, 2340, 2355, 2532, 3354
RsaNI	GTAC	6 cut(s)	551, 1998, 2339, 2354, 2531, 3353
RseI	CAYNNNNRTG	2 cut(s)	1287, 2678
SapI	GCTCTTC	1 cut(s)	576
SaqAI	TTAA	9 cut(s)	543, 1140, 1254, 1641, 1743, 1787, 2694, 2726, 3183
SchI	GAGTC	5 cut(s)	656, 1564, 1730, 2576, 3306
SduI	GDGCHC	4 cut(s)	280, 1249, 3153, 3284
SfaNI	GCATC	9 cut(s)	72, 131, 1204, 1279, 2115, 2321, 2455, 2765, 3185
SfcI	CTRYAG	8 cut(s)	267, 441, 1168, 1920, 2214, 2480, 3205, 3292
SfuI	TTCGAA	1 cut(s)	2515
SinI	GGWCC	9 cut(s)	101, 212, 371, 434, 524, 814, 839, 1733, 2187
SmiMI	CAYNNNNRTG	2 cut(s)	1287, 2678
SseBI	AGGCCT	2 cut(s)	949, 2832
SspMI	CTAG	6 cut(s)	654, 1011, 1679, 2100, 2429, 3192
StuI	AGGCCT	2 cut(s)	949, 2832
StyI	CCWWGG	6 cut(s)	105, 757, 944, 1771, 2167, 3274
TaaI	ACNGT	4 cut(s)	833, 1023, 1398, 2281
TaiI	ACGT	8 cut(s)	433, 966, 1419, 2162, 2778, 3132, 3179, 3358
TaqI	TCGA	4 cut(s)	2515, 2736, 2844, 3300
TatI	WGTACW	2 cut(s)	2338, 2530
TauI	GCSGC	4 cut(s)	65, 1381, 1393, 2666
TfiI	GAWTC	5 cut(s)	587, 636, 1652, 2905, 3347
Tru1I	TTAA	9 cut(s)	543, 1140, 1254, 1641, 1743, 1787, 2694, 2726, 3183
Tru9I	TTAA	9 cut(s)	543, 1140, 1254, 1641, 1743, 1787, 2694, 2726, 3183
TscAI	CASTG	9 cut(s)	55, 738, 1300, 1401, 1809, 1972, 1984, 2127, 2314
TseFI	GTSAC	6 cut(s)	148, 959, 1201, 1967, 2410, 2643
Tsp45I	GTSAC	6 cut(s)	148, 959, 1201, 1967, 2410, 2643
TspDTI	ATGAA	8 cut(s)	81, 159, 308, 579, 1013, 1980, 2048, 2094
TspGWI	ACGGA	1 cut(s)	884
TspRI	CASTG	9 cut(s)	55, 738, 1300, 1401, 1809, 1972, 1984, 2127, 2314
Van91I	CCANNNNNTGG	3 cut(s)	1118, 1814, 2458
VneI	GTGCAC	1 cut(s)	3149
VpaK11BI	GGWCC	9 cut(s)	101, 212, 371, 434, 524, 814, 839, 1733, 2187
XagI	CCTNNNNNAGG	1 cut(s)	468
XapI	RAATTY	8 cut(s)	28, 613, 1349, 1925, 2041, 2510, 3002, 3266
XceI	RCATGY	3 cut(s)	149, 823, 2644
XspI	CTAG	6 cut(s)	654, 1011, 1679, 2100, 2429, 3192
ZraI	GACGTC	1 cut(s)	2776

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.