Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3A

Orthogroup ID

OG0004774

Physical Location & Seq

Genomic Coordinates Forward (+)

6897934 .. 6905987

Gene Structure 8054 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3AG085700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGGGGACTGAAAATCCTGGTCGACCAAATTTTACTCCAAGACCTACCACTACACCTTTTGCTGCTCCTCCTCGGACCATGACGCCTTTTACATCTTCAGGTCCTGTGGTTGGACAAGAGGCATCTGGTTTTAGACCACCTCCACATACACCTTTCTCTTCGTCTGGGCCTGTGGTTGGATCAGATGTTTCTACCTTCAGACCTACTCCACCAGCTCCTCCACAGGCTACCATGCCTTTCTCATCTTTTGGGCCTGCAGGTGGACCACAGACCTCTCCCTTTAGGCCTAGCACACCAGCTAGGTTTAATGATCCATCCGTGCCTCCTCCACCAACAGCAAATGCCCCACCGACTGCTGGATCTTTCTCCCGTTTCCCGACTCCACCATTTTCTTCAACACCACAGTTTCCTTCGACAGCGCCACCACCCCCTTCCCGTCCTCCACCTATGGGCCAACTACCTTTTCAACCTCCAGGAGGTCAAGCTCCATTTCAACGACCACAGCAGCAGATGCCTTCTGTACAAATGGGATCTCCACCTCAAACTATAAATTCTGCTCCACCAGGTATGAGTCTTCATCAGTCTCCATCAGATTCGTCCTTCCCAGCTCCCCATCCAAAGGCTCAGACATCTTTCCCGGGATATGCACGTGGAAATCCACAAGCCCCACCAGCTCAGTCTCCATTTCTTACTCATCAAGGAGGCTTTCCCGCTCCACCTGCAGCATCTTCCCCTTTCGCTGCTCAGCAAATGGGTTACGGAATACCTCCACCAGTGGCAGCTCCACTGGGTCTGCAGTCGCAACATCCTGGCTCCGGACCCCCACTTGGTGGTGTCCAAGCCTTGACAGAAGACTTCAGCTCATTATCTGTTGGATCTGTTCCCGGATCAATTGAACCTGGAATTGACCCTAAAGCTCTGCCGAGGCCTTTGTGTGGTGATGTGGAGCCAAAACCACTTGCTGAGATGTTCCCCTTGAACTGCAATCCTAGATTTTTAAGGCTTACAACGGGTGCAATACCAAGTTCCCAGTCTTTGTCGTCAAGGTGGCATCTACCTCTGGGTGCTGTTGTTTGTCCACTTGCCGAATCACCTGATGGGGAGGAAGTGCCAGTGGTTAATTTTGGTGCTGCTGGGATTATTCGCTGTAGAAGATGTCGGACATATGTAAACCCATATGTGACATTTACAGATGCTGGAAGAAAGTGGCGTTGCAATATCTGTGCTCTACTTAATGATGTTCCAGGCGATTACTTTGCCAATTTGGATGCCACTGGCAGAAGAATTGATACGGACCAGCGACCTGAGCTTACACATGGTAGTGTGGAATTTGTTGCTCCAACTGAATATATGGTGCGGGCTCCTATGCCGCCACTGTATTTTTTCCTCATCGACGTTTCTTCATCTGCAGTGAGAAGTGGTATGATTGAGGTTGTGGCCCAAACTATTCGGTCATGCTTGGATGACCTGCCTGGCCACCCCAGAACACAAATAGGGTTTGCAACTTTTGACAGCACAATACATTTTTATAATATGAAGTCTTCTTTAACTCAGCCTCAAATGATGGTGGTCTCAGATTTGGATGATGTATTTGTCCCATTGCCAGATGATCTTCTGGTCAACTTATCTGAATCAAGAAGTGTTGTGGAGACCTTCCTAGATAGCTTGCCGTCTATGTTCCAGGACAACTCAAATGTGGAGTCTGCTTTTGGTCCAGCTCTTAAAGCCTCACTTATGCTTATGAGTCAACTTGGTGGAAAATTGTTGATTTTTCAAAATACACTTCCATCTCTTGGTGTTGGCCGCTTGAAATTGCGTGGAGATGATCTTCGTGTCTATGGAACAGATAAAGAACATCCTCTAAGACTACCAGAAGATCCTTTCTACAAGCAGATGGCTGCAGAATTTACCAAGTTCCAGATAGGAGTAAACGTATATGCCTTCAGTGACAAGTACACTGATATAGCCTCATTAGGTACTCTGGCAAAATATACTGGGGGTCAGGTGTACTATTACCCAAATTTCCAATCTACCATTCATGGAGAGAAGTTGAGACATGAGTTAGCCAGAGATCTTACTAGGGAAACTGCCTGGGAGGCAGTCATGCGCATAAGATGTGGAAAAGGAGTCCGCTTTACATCTTATCACGGGAACTTTATGCTGAGGTCCACCGATTTATTAGCACTTCCAGCTGTAGATTGTGATAAAGCATTTGCGATGCAGCTCTCACTTGAAGAGACATTACTAACTATTCAAACAGTATACTTCCAAGTAGCTTTACTATATACTGCATCCTGTGGAGAAAGGCGTATTAGAGTACATACAGCAGCAGTACCTGTTGTTACAGATCTGGGAGAGATGTATCGCCAGGCTGACACTGGTGCTATTGTCACCTTGCTTTCCAGGCTAGCTATAGAAAAGACTTTGTCGACTAAGTTGGAAGATGCTCGGAACTCTTTGCAGCTTAGAATTGTTAAAGCCCTTAAAGAATTTAGAAATCTTTATGCTGTACAACACCGTTTGGGAGGGAAGATGATATATCCAGAATCTCTGAAGTTCTTACCTATGTATGGATTAGCACTTTGTAAGTCAGCACCTCTCCGTGGGGGGTATGCAGATGTATCACTTGATGAACGGTGTGCGGCAGGCCACACAATGATGACTCTACCAGTTAAAAAATTGTTGAAGCTTCTATACCCTAGTTTAATACGGCTGGATGAGTATCTTTTGAAGCCTTCTGCTGATGCTGATGACTTCAGCAGCATAGAGAATAGATTACCACTAGTAGCTGAGAGCTTAGATTCCAGAGGCCTTTACATATATGATGATGGGTTTCGCTTTGTTCTATGGTTTGGTAGAGTTCTTCCACCTGATATAGCGAAGAATCTTCTTGGGGCTGACTTTGCAGCCGAGTTATCAAAGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0006275 GO:0006996 GO:0007029 GO:0007030 GO:0007276 GO:0008150 GO:0008156 GO:0008361 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010256 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032504 GO:0032535 GO:0032875 GO:0032876 GO:0044703 GO:0045786 GO:0045934 GO:0048232 GO:0048519 GO:0048523 GO:0048609 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051704 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0080119 GO:0090066 GO:0090329 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

975

Amino Acids

105.84

Weight (kDa)

7.56

Isoelectric Point (pI)

57.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-Sec23_Sec24	PF04810	380 - 418	1.1e-17	Sec23/Sec24 zinc finger
Sec23_trunk	PF04811	455 - 691	2.8e-83	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	697 - 781	7.3e-25	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	792 - 895	2.9e-24	Sec23/Sec24 helical domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07100
fragaria_vesca	FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220
malus_domestica	MD04G1188900.v1.1 MD12G1201700.v1.1
prunus_persica	Prupe.6G306800_v2.0.a1 Prupe.6G306800_v2.0.a1 Prupe.6G306800_v2.0.a1
pyrus_communis	pycom04g16720 pycom04g16730 pycom12g18800 pycom12g18810
rosa_chinensis	RchiOBHm_Chr3g0458141
rosa_laevigata	RLG00000025175
rosa_multiflora	Rmu_sc0019452.1_g000001
rosa_roxburghii	Rroxscaffold_6G00421270
rosa_rugosa	Rorug03G0024700 Rorug03G0024800 Rorug03G0024900 Rorug03G0024900 Rorug03G0025000
rosa_samantha	Rh3AG085700 Rh3BG088400 Rh3CG089000 Rh3DG089400
rosa_wichuraiana	Rw3G007280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1530
AarI	CACCTGC	2 cut(s)	248, 727
Acc16I	TGCGCA	1 cut(s)	2108
Acc36I	ACCTGC	3 cut(s)	248, 727, 1476
AccB7I	CCANNNNNTGG	2 cut(s)	830, 1793
AccBSI	CCGCTC	1 cut(s)	713
AccI	GTMKAC	3 cut(s)	22, 2262, 2429
AccIII	TCCGGA	1 cut(s)	815
AciI	CCGC	6 cut(s)	711, 1357, 1370, 1804, 2131, 2642
AclWI	GGATC	7 cut(s)	187, 305, 367, 538, 883, 895, 1871
AcoI	YGGCCR	2 cut(s)	1474, 1801
AcsI	RAATTY	6 cut(s)	28, 550, 1328, 1904, 2020, 2489
AcuI	CTGAAG	6 cut(s)	81, 181, 841, 1927, 2573, 2740
AcvI	CACGTG	1 cut(s)	650
AcyI	GRCGYC	1 cut(s)	83
AdeI	CACNNNGTG	1 cut(s)	830
AfaI	GTAC	7 cut(s)	522, 1955, 1978, 2009, 2319, 2334, 2511
AhlI	ACTAGT	1 cut(s)	2782
AjuI	GAANNNNNNNTTGG	4 cut(s)	447, 479, 2019, 2051
Alw21I	GWGCWC	1 cut(s)	1228
Alw26I	GTCTC	6 cut(s)	588, 684, 1576, 1643, 2047, 2231
AlwI	GGATC	7 cut(s)	187, 305, 367, 538, 883, 895, 1871
AlwNI	CAGNNNCTG	3 cut(s)	104, 1196, 2336
Ama87I	CYCGRG	1 cut(s)	637
Aor13HI	TCCGGA	1 cut(s)	815
ApoI	RAATTY	6 cut(s)	28, 550, 1328, 1904, 2020, 2489
ArsI	GACNNNNNNTTYG	2 cut(s)	1685, 1717
AspLEI	GCGC	2 cut(s)	421, 2109
AsuC2I	CCSGG	3 cut(s)	638, 639, 885
AsuHPI	GGTGA	3 cut(s)	950, 1083, 2383
AsuNHI	GCTAGC	1 cut(s)	2407
AvaI	CYCGRG	1 cut(s)	637
AvaII	GGWCC	7 cut(s)	75, 101, 263, 818, 1294, 1712, 2166
BalI	TGGCCA	1 cut(s)	1476
BanII	GRGCYC	1 cut(s)	1363
BarI	GAAGNNNNNNTAC	6 cut(s)	1273, 1305, 2226, 2258, 2678, 2710
BbrPI	CACGTG	1 cut(s)	650
BbsI	GAAGAC	3 cut(s)	566, 858, 1533
Bbv12I	GWGCWC	1 cut(s)	1228
BbvCI	CCTCAGC	1 cut(s)	2162
BccI	CCATC	8 cut(s)	322, 595, 621, 1091, 1558, 1795, 1888, 2822
BceAI	ACGGC	2 cut(s)	1654, 2726
BcgI	CGANNNNNNTGC	2 cut(s)	1238, 1272
BcnI	CCSGG	3 cut(s)	638, 639, 885
BcoDI	GTCTC	6 cut(s)	588, 684, 1576, 1643, 2047, 2231
BcuI	ACTAGT	1 cut(s)	2782
BfaI	CTAG	8 cut(s)	288, 300, 990, 1658, 2079, 2408, 2700, 2783
BfmI	CTRYAG	8 cut(s)	255, 720, 794, 1147, 1407, 1899, 2193, 2412
BfoI	RGCGCY	1 cut(s)	422
BfuAI	ACCTGC	3 cut(s)	248, 727, 1476
BglI	GCCNNNNNGGC	1 cut(s)	2096
BglII	AGATCT	2 cut(s)	2071, 2347
BlpI	GCTNAGC	1 cut(s)	744
Bme18I	GGWCC	7 cut(s)	75, 101, 263, 818, 1294, 1712, 2166
BmeT110I	CYCGRG	1 cut(s)	637
BmiI	GGNNCC	4 cut(s)	814, 820, 948, 1362
BmrI	ACTGGG	3 cut(s)	797, 1024, 2004
BmtI	GCTAGC	1 cut(s)	2411
BmuI	ACTGGG	3 cut(s)	797, 1024, 2004
BpiI	GAAGAC	3 cut(s)	566, 858, 1533
BpmI	CTGGAG	1 cut(s)	456
Bpu10I	CCTNAGC	2 cut(s)	1305, 2162
Bpu1102I	GCTNAGC	1 cut(s)	744
BpuMI	CCSGG	3 cut(s)	638, 639, 885
BsaAI	YACGTR	1 cut(s)	650
BsaBI	GATNNNNATC	1 cut(s)	2721
BsaHI	GRCGYC	1 cut(s)	83
BsaI	GGTCTC	2 cut(s)	1576, 1643
BsaJI	CCNNGG	5 cut(s)	71, 637, 923, 2091, 2602
BsaWI	WCCGGW	1 cut(s)	815
BsaXI	ACNNNNNCTCC	2 cut(s)	1917, 1947
Bse1I	ACTGG	8 cut(s)	773, 792, 1030, 1112, 1279, 1999, 2383, 2669
Bse3DI	GCAATG	1 cut(s)	1598
Bse8I	GATNNNNATC	1 cut(s)	2721
BseAI	TCCGGA	1 cut(s)	815
BseDI	CCNNGG	5 cut(s)	71, 637, 923, 2091, 2602
BseGI	GGATG	9 cut(s)	314, 613, 805, 1273, 1468, 1588, 1855, 2291, 2722
BseJI	GATNNNNATC	1 cut(s)	2721
BseMI	GCAATG	1 cut(s)	1598
BseMII	CTCAG	9 cut(s)	638, 689, 758, 954, 1296, 1565, 1587, 2153, 2781
BseNI	ACTGG	8 cut(s)	773, 792, 1030, 1112, 1279, 1999, 2383, 2669
BseRI	GAGGAG	4 cut(s)	57, 60, 207, 315
BseYI	CCCAGC	2 cut(s)	604, 1133
BsiHKAI	GWGCWC	1 cut(s)	1228
BsiHKCI	CYCGRG	1 cut(s)	637
BsiSI	CCGG	3 cut(s)	638, 816, 885
BslFI	GGGAC	2 cut(s)	19, 1580
BsmAI	GTCTC	6 cut(s)	588, 684, 1576, 1643, 2047, 2231
BsmFI	GGGAC	2 cut(s)	19, 1580
Bso31I	GGTCTC	2 cut(s)	1576, 1643
BsoBI	CYCGRG	1 cut(s)	637
Bsp1286I	GDGCHC	2 cut(s)	1228, 1363
Bsp13I	TCCGGA	1 cut(s)	815
Bsp1407I	TGTACA	2 cut(s)	520, 2509
Bsp1720I	GCTNAGC	1 cut(s)	744
BspACI	CCGC	6 cut(s)	711, 1357, 1370, 1804, 2131, 2642
BspCNI	CTCAG	9 cut(s)	637, 688, 757, 955, 1297, 1564, 1586, 2154, 2782
BspEI	TCCGGA	1 cut(s)	815
BspLI	GGNNCC	4 cut(s)	814, 820, 948, 1362
BspMAI	CTGCAG	5 cut(s)	259, 724, 798, 1411, 1903
BspMI	ACCTGC	3 cut(s)	248, 727, 1476
BspOI	GCTAGC	1 cut(s)	2411
BspPI	GGATC	7 cut(s)	187, 305, 367, 538, 883, 895, 1871
BspTNI	GGTCTC	2 cut(s)	1576, 1643
BsrBI	CCGCTC	1 cut(s)	713
BsrDI	GCAATG	1 cut(s)	1598
BsrGI	TGTACA	2 cut(s)	520, 2509
BsrI	ACTGG	8 cut(s)	773, 792, 1030, 1112, 1279, 1999, 2383, 2669
BssECI	CCNNGG	5 cut(s)	71, 637, 923, 2091, 2602
BssNAI	GTATAC	1 cut(s)	2263
BssNI	GRCGYC	1 cut(s)	83
Bst1107I	GTATAC	1 cut(s)	2263
Bst4CI	ACNGT	5 cut(s)	405, 1377, 2260, 2519, 2637
Bst6I	CTCTTC	2 cut(s)	163, 2229
BstACI	GRCGYC	1 cut(s)	83
BstAPI	GCANNNNNTGC	1 cut(s)	511
BstAUI	TGTACA	2 cut(s)	520, 2509
BstBAI	YACGTR	1 cut(s)	650
BstC8I	GCNNGC	5 cut(s)	255, 1359, 1667, 2409, 2647
BstDSI	CCRYGG	1 cut(s)	2602
BstENI	CCTNNNNNAGG	1 cut(s)	474
BstF5I	GGATG	9 cut(s)	314, 613, 805, 1273, 1468, 1588, 1855, 2291, 2722
BstH2I	RGCGCY	1 cut(s)	422
BstHHI	GCGC	2 cut(s)	421, 2109
BstMAI	GTCTC	6 cut(s)	588, 684, 1576, 1643, 2047, 2231
BstMWI	GCNNNNNNNGC	9 cut(s)	511, 671, 719, 1306, 1724, 2096, 2189, 2404, 2903
BstSFI	CTRYAG	8 cut(s)	255, 720, 794, 1147, 1407, 1899, 2193, 2412
BstV2I	GAAGAC	3 cut(s)	566, 858, 1533
BstX2I	RGATCY	6 cut(s)	359, 530, 875, 1876, 2071, 2347
BstYI	RGATCY	6 cut(s)	359, 530, 875, 1876, 2071, 2347
BstZ17I	GTATAC	1 cut(s)	2263
BtgI	CCRYGG	1 cut(s)	2602
BtgZI	GCGATG	1 cut(s)	2231
BtsCI	GGATG	9 cut(s)	314, 613, 805, 1273, 1468, 1588, 1855, 2291, 2722
BtsI	GCAGTG	1 cut(s)	1416
BtsIMutI	CAGTG	9 cut(s)	780, 785, 1119, 1272, 1373, 1416, 1951, 1956, 2376
BveI	ACCTGC	3 cut(s)	248, 727, 1476
Cac8I	GCNNGC	5 cut(s)	255, 1359, 1667, 2409, 2647
CaiI	CAGNNNCTG	3 cut(s)	104, 1196, 2336
CfoI	GCGC	2 cut(s)	421, 2109
Cfr9I	CCCGGG	1 cut(s)	637
CseI	GACGC	1 cut(s)	91
CsiI	ACCWGGT	1 cut(s)	562
Csp6I	GTAC	7 cut(s)	521, 1954, 1977, 2008, 2318, 2333, 2510
CviAII	CATG	7 cut(s)	79, 232, 1316, 1455, 2039, 2057, 2104
CviQI	GTAC	7 cut(s)	521, 1954, 1977, 2008, 2318, 2333, 2510
DraIII	CACNNNGTG	1 cut(s)	830
EaeI	YGGCCR	2 cut(s)	1474, 1801
Eam1104I	CTCTTC	2 cut(s)	163, 2229
EarI	CTCTTC	2 cut(s)	163, 2229
Eco147I	AGGCCT	3 cut(s)	286, 928, 2811
Eco24I	GRGCYC	1 cut(s)	1363
Eco31I	GGTCTC	2 cut(s)	1576, 1643
Eco47I	GGWCC	7 cut(s)	75, 101, 263, 818, 1294, 1712, 2166
Eco57I	CTGAAG	6 cut(s)	81, 181, 841, 1927, 2573, 2740
Eco72I	CACGTG	1 cut(s)	650
Eco88I	CYCGRG	1 cut(s)	637
EcoNI	CCTNNNNNAGG	1 cut(s)	474
EcoO109I	RGGNCCY	1 cut(s)	101
EcoT38I	GRGCYC	1 cut(s)	1363
FaeI	CATG	7 cut(s)	82, 235, 1319, 1458, 2042, 2060, 2107
FalI	AAGNNNNNCTT	2 cut(s)	1716, 1748
FaqI	GGGAC	2 cut(s)	19, 1580
FatI	CATG	7 cut(s)	78, 231, 1315, 1454, 2038, 2056, 2103
FauI	CCCGC	2 cut(s)	718, 1350
FauNDI	CATATG	2 cut(s)	1165, 1177
FblI	GTMKAC	3 cut(s)	22, 2262, 2429
FokI	GGATG	9 cut(s)	301, 600, 792, 1280, 1475, 1595, 1842, 2278, 2729
FriOI	GRGCYC	1 cut(s)	1363
FspAI	RTGCGCAY	1 cut(s)	2108
FspBI	CTAG	8 cut(s)	288, 300, 990, 1658, 2079, 2408, 2700, 2783
FspI	TGCGCA	1 cut(s)	2108
GlaI	GCGC	2 cut(s)	420, 2108
GsaI	CCCAGC	2 cut(s)	608, 1137
GsuI	CTGGAG	1 cut(s)	456
HaeII	RGCGCY	1 cut(s)	422
HapII	CCGG	3 cut(s)	638, 816, 885
HgaI	GACGC	1 cut(s)	91
HhaI	GCGC	2 cut(s)	421, 2109
Hin1I	GRCGYC	1 cut(s)	83
Hin1II	CATG	7 cut(s)	82, 235, 1319, 1458, 2042, 2060, 2107
Hin6I	GCGC	2 cut(s)	419, 2107
HinP1I	GCGC	2 cut(s)	419, 2107
HincII	GTYRAC	4 cut(s)	23, 1621, 1748, 2430
HindII	GTYRAC	4 cut(s)	23, 1621, 1748, 2430
HindIII	AAGCTT	1 cut(s)	2687
HpaII	CCGG	3 cut(s)	638, 816, 885
HphI	GGTGA	3 cut(s)	950, 1083, 2383
Hpy188III	TCNNGA	6 cut(s)	376, 816, 1635, 1918, 2543, 2805
Hpy99I	CGWCG	1 cut(s)	1397
HpyAV	CCTTC	8 cut(s)	205, 420, 441, 525, 610, 1663, 1951, 2745
HpyCH4III	ACNGT	5 cut(s)	405, 1377, 2260, 2519, 2637
HpyCH4IV	ACGT	3 cut(s)	649, 1395, 1932
HpyF10VI	GCNNNNNNNGC	9 cut(s)	511, 671, 719, 1306, 1724, 2096, 2189, 2404, 2903
HpySE526I	ACGT	3 cut(s)	649, 1395, 1932
Hsp92I	GRCGYC	1 cut(s)	83
Hsp92II	CATG	7 cut(s)	82, 235, 1319, 1458, 2042, 2060, 2107
HspAI	GCGC	2 cut(s)	419, 2107
Kpn2I	TCCGGA	1 cut(s)	815
MabI	ACCWGGT	1 cut(s)	562
MaeI	CTAG	8 cut(s)	288, 300, 990, 1658, 2079, 2408, 2700, 2783
MaeII	ACGT	3 cut(s)	649, 1395, 1932
MaeIII	GTNAC	5 cut(s)	755, 1180, 1946, 2341, 2389
MbiI	CCGCTC	1 cut(s)	713
MfeI	CAATTG	1 cut(s)	891
MflI	RGATCY	6 cut(s)	359, 530, 875, 1876, 2071, 2347
MhlI	GDGCHC	2 cut(s)	1228, 1363
MlsI	TGGCCA	1 cut(s)	1476
MluNI	TGGCCA	1 cut(s)	1476
MlyI	GAGTC	6 cut(s)	373, 580, 1709, 1753, 2136, 2656
MmeI	TCCRAC	6 cut(s)	91, 157, 853, 1139, 1364, 2418
Mox20I	TGGCCA	1 cut(s)	1476
MroI	TCCGGA	1 cut(s)	815
MscI	TGGCCA	1 cut(s)	1476
MslI	CAYNNNNRTG	2 cut(s)	1320, 2657
Msp20I	TGGCCA	1 cut(s)	1476
MspA1I	CMGCKG	1 cut(s)	2192
MspI	CCGG	3 cut(s)	638, 816, 885
MunI	CAATTG	1 cut(s)	891
MwoI	GCNNNNNNNGC	9 cut(s)	511, 671, 719, 1306, 1724, 2096, 2189, 2404, 2903
NciI	CCSGG	3 cut(s)	638, 639, 885
NdeI	CATATG	2 cut(s)	1165, 1177
NheI	GCTAGC	1 cut(s)	2407
NlaIII	CATG	7 cut(s)	82, 235, 1319, 1458, 2042, 2060, 2107
NlaIV	GGNNCC	4 cut(s)	814, 820, 948, 1362
NmeAIII	GCCGAG	1 cut(s)	948
NmuCI	GTSAC	3 cut(s)	1180, 1946, 2389
NsbI	TGCGCA	1 cut(s)	2108
PaqCI	CACCTGC	2 cut(s)	248, 727
PceI	AGGCCT	3 cut(s)	286, 928, 2811
PfeI	GAWTC	6 cut(s)	593, 1088, 1631, 2546, 2801, 2884
PflFI	GACNNNGTC	1 cut(s)	2425
PflMI	CCANNNNNTGG	2 cut(s)	830, 1793
PfoI	TCCNGGA	3 cut(s)	472, 883, 1680
PleI	GAGTC	6 cut(s)	373, 579, 1708, 1752, 2135, 2656
PmaCI	CACGTG	1 cut(s)	650
PmlI	CACGTG	1 cut(s)	650
PpsI	GAGTC	6 cut(s)	373, 579, 1708, 1752, 2135, 2656
Ppu21I	YACGTR	1 cut(s)	650
PpuMI	RGGWCCY	1 cut(s)	101
PsiI	TTATAA	1 cut(s)	1530
Psp5II	RGGWCCY	1 cut(s)	101
PspCI	CACGTG	1 cut(s)	650
PspFI	CCCAGC	2 cut(s)	604, 1133
PspN4I	GGNNCC	4 cut(s)	814, 820, 948, 1362
PspPPI	RGGWCCY	1 cut(s)	101
PstI	CTGCAG	5 cut(s)	259, 724, 798, 1411, 1903
PstNI	CAGNNNCTG	3 cut(s)	104, 1196, 2336
PsuI	RGATCY	6 cut(s)	359, 530, 875, 1876, 2071, 2347
PsyI	GACNNNGTC	1 cut(s)	2425
PvuII	CAGCTG	1 cut(s)	2192
RsaI	GTAC	7 cut(s)	522, 1955, 1978, 2009, 2319, 2334, 2511
RsaNI	GTAC	7 cut(s)	521, 1954, 1977, 2008, 2318, 2333, 2510
RseI	CAYNNNNRTG	2 cut(s)	1320, 2657
SalI	GTCGAC	2 cut(s)	21, 2428
SbfI	CCTGCAGG	1 cut(s)	259
SchI	GAGTC	6 cut(s)	373, 580, 1709, 1753, 2136, 2656
SdaI	CCTGCAGG	1 cut(s)	259
SduI	GDGCHC	2 cut(s)	1228, 1363
SexAI	ACCWGGT	1 cut(s)	562
SfcI	CTRYAG	8 cut(s)	255, 720, 794, 1147, 1407, 1899, 2193, 2412
SinI	GGWCC	7 cut(s)	75, 101, 263, 818, 1294, 1712, 2166
SmaI	CCCGGG	1 cut(s)	639
SmiMI	CAYNNNNRTG	2 cut(s)	1320, 2657
SpeI	ACTAGT	1 cut(s)	2782
Sse8387I	CCTGCAGG	1 cut(s)	259
SseBI	AGGCCT	3 cut(s)	286, 928, 2811
SsiI	CCGC	6 cut(s)	711, 1357, 1370, 1804, 2131, 2642
SspMI	CTAG	8 cut(s)	288, 300, 990, 1658, 2079, 2408, 2700, 2783
StuI	AGGCCT	3 cut(s)	286, 928, 2811
TaaI	ACNGT	5 cut(s)	405, 1377, 2260, 2519, 2637
TaiI	ACGT	3 cut(s)	652, 1398, 1935
TaqI	TCGA	4 cut(s)	22, 413, 1392, 2429
TaqII	GACCGA	1 cut(s)	1440
TatI	WGTACW	5 cut(s)	520, 1953, 2007, 2317, 2509
TauI	GCSGC	3 cut(s)	1372, 1806, 2645
TfiI	GAWTC	6 cut(s)	593, 1088, 1631, 2546, 2801, 2884
TscAI	CASTG	9 cut(s)	780, 792, 1119, 1279, 1380, 1416, 1951, 1963, 2383
TseFI	GTSAC	3 cut(s)	1180, 1946, 2389
Tsp45I	GTSAC	3 cut(s)	1180, 1946, 2389
TspDTI	ATGAA	5 cut(s)	566, 1392, 1550, 2027, 2646
TspGWI	ACGGA	4 cut(s)	307, 774, 1307, 2591
TspMI	CCCGGG	1 cut(s)	637
TspRI	CASTG	9 cut(s)	780, 792, 1119, 1279, 1380, 1416, 1951, 1963, 2383
Tth111I	GACNNNGTC	1 cut(s)	2425
Van91I	CCANNNNNTGG	2 cut(s)	830, 1793
VpaK11BI	GGWCC	7 cut(s)	75, 101, 263, 818, 1294, 1712, 2166
XagI	CCTNNNNNAGG	1 cut(s)	474
XapI	RAATTY	6 cut(s)	28, 550, 1328, 1904, 2020, 2489
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2375
XmaI	CCCGGG	1 cut(s)	637
XmiI	GTMKAC	3 cut(s)	22, 2262, 2429
XspI	CTAG	8 cut(s)	288, 300, 990, 1658, 2079, 2408, 2700, 2783

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.