Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0004774

Physical Location & Seq

Genomic Coordinates Reverse (-)

42743814 .. 42753068

Gene Structure 9255 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025175

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5319 bp

ATGGGGACTGAAAATCCTGGTCGACCAAATTTTACTCCAAGACCTACCACTACACCTTTTGCTGCTCCTCCTCGGACCATGACGCCTTTTACATCTTCAGGTCCTGTGGTTGGACAAGAGGCATCTGGTTTTAGACCACCTCCACAGACACCTTTCTCTTCGTCTGGGCCTGTGGTTGGATCAGATGTTTCTACCTTCAGACCTACTCCACCAGCTCCTCCACAGGCTACCATGCCTTTCTCATCTTTTGGGCCTGCAAGTGGACCACAGACCTCTCCCTTTAGGCCTAGCACACCAGCTAGGTTTAATGATCCATCTGTGCCTCCTCCACCAACAGCAAATGCTCCACCGACTGCTGGACCTTTCTCCCGTTTCCCGACTCCACCATTTTCTTCAACACCACAGTATTCGACAGCGCCACCACCCCCTTCCCGTCCTCCACCTATGGGCCAACTACCTTTTCAACCTCCAGGAGGTCGAGCTCCATTTCAACGACCACAGCAGCAGATGCCTTCTGTACAAATGGGATCTCCACCTCAAACTATAAATTCTGCTCCACCAGGTATGAGTGTTCATCAGTCTCCATCAGATTCGTCCTTCCCAGCTCCCCAGCCAAAGGCTCAGACATCTTTCCCGGGATATGCACGTGCAAATCCACAAGGCCCGCCAGCTCAGTCTCCATTTCTTACTCATCAAGGAGGCTTTCCCGCTCCACCTGCAGCATCTTCCCCTTTCGCTGCTCAGCAAGTGGGTTATGGAGTACCTCCACCAGTGGCAGCTCCACTGGGTCTGCAGTCGCAACATCCTGGCTCCGGACCCCCACTTGGTGGTGTCCAAGCCTTGACAGAGGACTTCAGCTCATTATCTGTTGGATCTGTTCCCGGATCAATTGAACCTGGAATTGACCCTAAAGCTCTGCCGAGGCCTTTGTGTGGTGATGTGGAGCCAAAACCACTTGCTGAGATGTTCCCCTTGAACTGCAATCCTAGATTTTTAAGGCTTACAACGGGTGCAATACCAAGTTCCCAGTCTTTGTTGTCAAGGTGGCATCTACCTCTGGGTGCTGTTGTTTGTCCACTTGCCGAATCACCTGATGGGGAGGAAGTGCCGGTGGTTAATTTTGGTGCTGCTGGGATTATTCGCTGTAGAAGATGTCGCACATATGTAAACCCATATGTGACATTTACAGATGCTGGAAGAAAGTGGCGTTGCAATATCTGTGCTCTACTTAATGATGTTCCAGGCGATTACTTTGCCAATTTGGATGCCACTGGCAGAAGAATTGATATGGACCAGCGACCTGAGCTTACACATGGTAGTGTGGAATTTGTTGCTCCAACTGAATATATGGTGCGGGCTCCTATGCCGCCACTGTATTTTTTCCTCATCGACGTTTCTTCATCTGCAGTGAGAAGTGGTATGATTGAGGTTGTGGCCCAAACTATTCGGTCATGCTTGGATGACCTGCCTGGCCACCCCAGAACACAAATAGGGTTTGCAACTTTTGACAGCACAATACATTTTTATAATATGAAGTCTTCTTTAACTCAGCCTCAAATGATGGTGGTCTCAGATTTGGATGACGTATTTGTCCCATTGCCAGATGATCTTCTGGTCAACTTATCTGAATCAAGAAGTGTTGTGGAGACCTTTCTAGATAGCTTGCCGTCTATGTTCCAGGACAACTCAAATGTGGAGTCTGCTTTTGGTCCAGCTCTTAAAGCCTCACTTATGCTTATGAGTCAACTTGGTGGAAAATTGTTGATTTTTCAAAATACACTTCCATCTCTTGGTGTTGGCCGCTTGAAATTGCGTGGAGATGATCTTCGTGTCTATGGAACAGATAAAGAACATCCTCTAAGACTACCAGAAGATCCTTTCTACAAGCAGATGGCTGCAGAATTTACCAAGTTCCAGATAGGAGTAAACGTATATGCCTTCAGTGACAAGTACACTGATATAGCCTCATTAGGTACTCTGGCAAAATATACCGGGGGTCAGGTGTACTATTACCCAAATTTCCAATCTACCATTCATGGAGAGAAGTTGAGACATGAGTTAGCCAGAGATCTTACTAGGGAAACTGCCTGGGAGGCAGTCATGCGCATAAGATGTGGAAAAGGAGTCCGCTTTACATCTTATCACGGGAACTTTATGCTGAGGTCCACCGATTTATTAGCACTTCCAGCTGTAGATTGTGATAAAGCATTTGCTATGCAGCTCTCACTTGAAGAGACATTACTAACTATTCAGACAGTATACTTCCAAGTAGCTTTACTATACACTGCATCCTGCGGAGAAAGGCGTATTAGAGTACATACAGCAGCAGTACCTGTTGTTACAGATCTGGGAGAGATGTATCGCCAGGCTGACACTGGTGCTATTGTCACCTTGCTTTCCAGGCTAGCAATAGAAAAGACTTTGTCGAGTAAGTTGGAAGATGCTCGGAACTCTTTGCAGCTTAGAATTGTCAAAGCCCTTAAAGAATTTAGAAATCTTTATGCTGTACAACACCGTTTGGGAGGGAAGATGATATATCCAGAGTCTCTGAAGTTCTTACCTATGTATGGATTAGCACTTTGTAAGTCAGCACCTCTCCGTGGGGGGTATGCAGATGTATCACTTGATGAACGGTGTGCGGCAGGCCACACAATGATGACTCTACCAGTTAAAAAATTGTTGAAGCTTCTATACCCTAGTTTAATACGGCTCGATGAGTATCTTTTGAAGCCTTCTGCTGATGCTGATGACTTCAACAGCATAGAGAATAGATTACCACTAGTAGCTGAGAGCTTAGATTCCAGAGGCCTTTACATATATGATGATGGGTTTCGCTTTGTTCTATGGTTTGGTAGAGTTCTTCCACCTGATATAGCGAAGAATCTTCTTGGGGCTGACTTTGCAGCTGAGTTATCGAAGGTCACCCTTTGTGAGCGTGATAATGAAATGTCAAAGAAGCTAATGAGGATACTTAAGAAATTTAGAGAAAGTGATCCTTCATATCATCAGTTGTGTTATCTTGTAAGACAAGGAGAGCAGCCACGAGAAGGGGCTCACTTCCTTGAAGTTCAAGCAATCAAGCCTACTTGTACTAATAATAGAACGTTTGGTGCATTCCGAAGCGTGGTGATGGGTGTGAGTTTGACTCTGATCAAGAGAGTCTCTCTGCTTCCTCTTCCCGCCAAATTCCGAACTTTGTCTCAGTTTTCCTTCACCTTCTCCAATCACTATTGCTCCTCTCCTCTCAACCTAGACTCATACTTCTCCGACGACCATACCCTCCATTTCACCTACGATTCCGAGCCATTCTTTGCCTCTCTCATCGATAATTCCACCCACAAGACCCACTTGAATCAAATCCAAGCGCAGTTACTTGTACTGGGTTTCCAAGCCAGCAGCTTTTTAATCACCAAATTGGTTAACTGCAGCTCAAACCTCGGGTGCATTTCCTATGCCCGCCAGGTGTTCGATGAATTCACTGACCCGAATGTGTTCCTGTGGAATGCAATCGTTCGGGGCTATTCGAAGCACAATGCGTTTGCTGATGCTATAGAAATGTATTGTAGGATGCAAGAGACCGGTGTGAGTCCAGATGGTTTTACTTTCCCTCATGTGCTCAAAGCTTGCAGCGCCTTGGCGGCGCTTGAAATGGGCCGCCGGGTTCACTGCCAGGTGTTTCGAAATGGTTTTGAGTCGGATGTGTTTGTGCAGAACAGTCTCGTGGCGTTTTATGCCAAATGTGGTAGGATGGAACGTGCTAGGATTGTGTTTGATGGGTTAGGTGAGAGGTCTATTGTTTCGTGGACTTCGATCATTTCAGGGTATGCGCAGAATGGCCAGTCTTTGGAAGCTCTGAGAATTTTTAGTGTGATGAGAAAGTTGAGTGTAAAACCGGATTGGATTGTTCTGGTTAGTGTTCTCAAAGCGTATACTGATGTAGAGGACTTGGGACAGGGGAGGTCTGTTCATGGTTGTTTAGTTAAAATGGGACTTGAGTTTGAACCTGACTTGGTCATTTCACTCACAGCCATGTATGCAAAAAGCGGGCAGGTGATGGTTGCCAGATCATTCTTTGATAAGATGGAAAGACCAAATGTAATGATGTGGAATGCTATGATTTCTGGTTATGCGAAGAATGGTCATGCTGAGAAAGCTGTAGAGCTATTCCGTGAGATGATCTCTAGAAGCATTAGCATTGATTCAATTACTCTGAGGTCAGCTATCTTAGCATGCGCCCAGGTGGGTTCTCTTGAATTAGCGAAATGGATGGATGATTATATCAGCAAAAGTGAGTATAGAAATCATGTTTTTGTTAACACTGCCCTAATTGATATGTATGCAAAATGTGGTAGTGTAGATTTTGCTCGCATGGTCTTTGATAGGACACCTAATAAAGATGTCATCGTCTGGAGTGCAATGATTGTGGGCTATGGATTGCATGGTCGAGGACAAGAAGCCATTGATGTTTACTATTCGATGCAGCAGGCTGGGGTGGTACCAAATGATGTTACCTTTCTTGGACTCCTTACTGCATGCAACCATTCAGGCCTTGTACATGAGGGATGGGAGCTTTTCCACCGAATGAGAGACTATGGGATTGAGCCTGGAAACCATCATTATTCATGTGTGGTTGATCTTCTGGGACGTGCAGGCCAGTTGGAACAGGCTAATGATTTTATAAAAAAGATGCCAATTGAACCTGGTACAAGTGTATGGGGAGCACTTCTTAGTTCATGCAAGATCCATCGAAATGTGACTTTGGGAGAATATGCTGCAGAAAAGCTTTTCTTATTAGATCCATATGAGATAGGGCATTATGTTCAACTGTCAAATCTCTATGCTTCAGCCCGATTATGGGATCGTGTTGCACAGGTGCGAGTACTGATGAGGGAGAAAGGACTGATCAAGGGCCTTGGACATAGTTTGATTGAGATCAATGGAAAACTTCAGGCATTTCATGTAGGAGACAAGTCACATCCAAGGTCTAAGGAAATATATGAAGAGCTTGAGAGTCTAGAGAGGAGGTTGAAGGAGGCTGGATTTGTTCCACGTACAGAATCAGTCCTGCATGATTTGAATTATGAAGAGACAGAGGAGACACTTTGCAATCATAGTGAGAGGCTGGCAATTGCTTATGGCCTCATCTGTACTGGTCCTGGAACTACACTAAGAATAACTAAAAACCTACGCGCGTGTGTGAATTGTCATGCAGCAACTAAGCTAATATCTAAGCTTGTCAACAGGGAGATAGTTGTCAGGGATGCAAAGCGTTTCCACCATTTCAGGGATGGATTTTGTTCCTGTGGAGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0006275 GO:0006996 GO:0007029 GO:0007030 GO:0007276 GO:0008150 GO:0008156 GO:0008361 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010256 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032504 GO:0032535 GO:0032875 GO:0032876 GO:0044703 GO:0045786 GO:0045934 GO:0048232 GO:0048519 GO:0048523 GO:0048609 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051704 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0080119 GO:0090066 GO:0090329 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

1773

Amino Acids

196.04

Weight (kDa)

8.01

Isoelectric Point (pI)

49.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-Sec23_Sec24	PF04810	379 - 417	2.2e-17	Sec23/Sec24 zinc finger
Sec23_trunk	PF04811	454 - 690	7.2e-83	Sec23/Sec24 trunk domain
Sec23_BS	PF08033	696 - 780	1.5e-24	Sec23/Sec24 beta-sandwich domain
Sec23_helical	PF04815	791 - 894	2.7e-24	Sec23/Sec24 helical domain
Gelsolin	PF00626	926 - 991	4.7e-06	Gelsolin repeat
PPR_2	PF13041	1162 - 1209	7.8e-10	PPR repeat family
PPR_2	PF13041	1264 - 1309	1.6e-07	PPR repeat family
PPR	PF01535	1266 - 1294	3.5e-06	PPR repeat
PPR_2	PF13041	1364 - 1412	9e-12	PPR repeat family
PPR_1	PF12854	1364 - 1392	1.1e-07	PPR repeat
PPR	PF01535	1368 - 1396	1.4e-09	PPR repeat
PPR_2	PF13041	1465 - 1512	7.9e-08	PPR repeat family
E_motif	PF20431	1582 - 1644	8.4e-11	E motif
Eplus_motif	PF20430	1652 - 1676	4.7e-09	E+ motif
DYW_deaminase	PF14432	1680 - 1772	5.9e-39	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004774)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07100
fragaria_vesca	FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220 FvH4_6g08220
malus_domestica	MD04G1188900.v1.1 MD12G1201700.v1.1
prunus_persica	Prupe.6G306800_v2.0.a1 Prupe.6G306800_v2.0.a1 Prupe.6G306800_v2.0.a1
pyrus_communis	pycom04g16720 pycom04g16730 pycom12g18800 pycom12g18810
rosa_chinensis	RchiOBHm_Chr3g0458141
rosa_laevigata	RLG00000025175
rosa_multiflora	Rmu_sc0019452.1_g000001
rosa_roxburghii	Rroxscaffold_6G00421270
rosa_rugosa	Rorug03G0024700 Rorug03G0024800 Rorug03G0024900 Rorug03G0024900 Rorug03G0025000
rosa_samantha	Rh3AG085700 Rh3BG088400 Rh3CG089000 Rh3DG089400
rosa_wichuraiana	Rw3G007280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1527, 4679
AarI	CACCTGC	2 cut(s)	724, 4039
AasI	GACNNNNNNGTC	1 cut(s)	1589
Acc16I	TGCGCA	2 cut(s)	2105, 3828
Acc36I	ACCTGC	3 cut(s)	724, 1473, 4039
Acc65I	GGTACC	1 cut(s)	4495
AccB1I	GGYRCC	1 cut(s)	4495
AccB7I	CCANNNNNTGG	3 cut(s)	827, 1790, 3844
AccBSI	CCGCTC	1 cut(s)	710
AccI	GTMKAC	3 cut(s)	22, 2259, 3929
AccII	CGCG	2 cut(s)	5190, 5192
AccIII	TCCGGA	1 cut(s)	812
AclI	AACGTT	1 cut(s)	3104
AcoI	YGGCCR	3 cut(s)	1471, 1798, 3835
AcuI	CTGAAG	7 cut(s)	81, 181, 838, 1924, 2570, 4827, 4931
AcvI	CACGTG	1 cut(s)	647
AcyI	GRCGYC	1 cut(s)	83
AdeI	CACNNNGTG	1 cut(s)	827
AflII	CTTAAG	1 cut(s)	2972
AgeI	ACCGGT	1 cut(s)	3578
AhlI	ACTAGT	1 cut(s)	2779
AjiI	CACGTC	1 cut(s)	4646
AjuI	GAANNNNNNNTTGG	4 cut(s)	444, 476, 2016, 2048
Alw21I	GWGCWC	4 cut(s)	484, 1225, 3618, 4723
AlwNI	CAGNNNCTG	3 cut(s)	104, 1193, 2333
Ama87I	CYCGRG	2 cut(s)	634, 3437
Aor13HI	TCCGGA	1 cut(s)	812
ArsI	GACNNNNNNTTYG	2 cut(s)	1682, 1714
AsiGI	ACCGGT	1 cut(s)	3578
Asp700I	GAANNNNTTC	1 cut(s)	3491
Asp718I	GGTACC	1 cut(s)	4495
AspLEI	GCGC	8 cut(s)	418, 2106, 3367, 3632, 3643, 3829, 4235, 5192
AsuC2I	CCSGG	5 cut(s)	635, 636, 882, 1993, 3659
AsuII	TTCGAA	2 cut(s)	3524, 3679
AsuNHI	GCTAGC	1 cut(s)	2404
AvaI	CYCGRG	2 cut(s)	634, 3437
AvaII	GGWCC	9 cut(s)	75, 101, 263, 359, 815, 1291, 1709, 2163, 5153
BaeI	ACNNNNGTAYC	2 cut(s)	4695, 4728
BalI	TGGCCA	2 cut(s)	1473, 3837
BanI	GGYRCC	1 cut(s)	4495
BanII	GRGCYC	3 cut(s)	484, 1360, 3055
BarI	GAAGNNNNNNTAC	4 cut(s)	2223, 2255, 2675, 2707
BauI	CACGAG	2 cut(s)	3042, 3719
BbrPI	CACGTG	1 cut(s)	647
BbsI	GAAGAC	1 cut(s)	1530
Bbv12I	GWGCWC	4 cut(s)	484, 1225, 3618, 4723
BbvCI	CCTCAGC	1 cut(s)	2159
BceAI	ACGGC	2 cut(s)	1651, 2723
BcgI	CGANNNNNNTGC	2 cut(s)	1235, 1269
BciVI	GTATCC	1 cut(s)	2961
BclI	TGATCA	2 cut(s)	3150, 4902
BcnI	CCSGG	5 cut(s)	635, 636, 882, 1993, 3659
BcuI	ACTAGT	1 cut(s)	2779
BfoI	RGCGCY	3 cut(s)	419, 3633, 3644
BfrI	CTTAAG	1 cut(s)	2972
BfuAI	ACCTGC	3 cut(s)	724, 1473, 4039
BfuI	GTATCC	1 cut(s)	2961
BglI	GCCNNNNNGGC	2 cut(s)	2093, 3638
BglII	AGATCT	2 cut(s)	2068, 2344
BlpI	GCTNAGC	1 cut(s)	741
BmcAI	AGTACT	1 cut(s)	4881
Bme18I	GGWCC	9 cut(s)	75, 101, 263, 359, 815, 1291, 1709, 2163, 5153
BmeT110I	CYCGRG	2 cut(s)	634, 3437
BmgBI	CACGTC	1 cut(s)	4646
BmiI	GGNNCC	5 cut(s)	811, 817, 945, 1359, 4497
BmrI	ACTGGG	3 cut(s)	794, 1021, 3389
BmtI	GCTAGC	1 cut(s)	2408
BmuI	ACTGGG	3 cut(s)	794, 1021, 3389
BpiI	GAAGAC	1 cut(s)	1530
BplI	GAGNNNNNCTC	6 cut(s)	3130, 3162, 3148, 3180, 4163, 4195
BpmI	CTGGAG	2 cut(s)	453, 4429
Bpu10I	CCTNAGC	2 cut(s)	1302, 2159
Bpu1102I	GCTNAGC	1 cut(s)	741
Bpu14I	TTCGAA	2 cut(s)	3524, 3679
BpuEI	CTTGAG	2 cut(s)	4013, 5027
BpuMI	CCSGG	5 cut(s)	635, 636, 882, 1993, 3659
Bsa29I	ATCGAT	1 cut(s)	3324
BsaAI	YACGTR	2 cut(s)	647, 5051
BsaBI	GATNNNNATC	2 cut(s)	2718, 4931
BsaHI	GRCGYC	1 cut(s)	83
BsaI	GGTCTC	3 cut(s)	1573, 1640, 3569
BsaWI	WCCGGW	3 cut(s)	812, 3578, 3892
BsaXI	ACNNNNNCTCC	4 cut(s)	1914, 1944, 3218, 3248
Bse118I	RCCGGY	2 cut(s)	1108, 3578
Bse3DI	GCAATG	2 cut(s)	1595, 4422
Bse8I	GATNNNNATC	2 cut(s)	2718, 4931
BseAI	TCCGGA	1 cut(s)	812
BseCI	ATCGAT	1 cut(s)	3324
BseJI	GATNNNNATC	2 cut(s)	2718, 4931
BseMI	GCAATG	2 cut(s)	1595, 4422
BseRI	GAGGAG	8 cut(s)	57, 60, 207, 315, 3226, 3231, 5035, 5108
BseYI	CCCAGC	4 cut(s)	601, 609, 1130, 4487
BsgI	GTGCAG	2 cut(s)	3728, 4668
Bsh1236I	CGCG	2 cut(s)	5190, 5192
BshNI	GGYRCC	1 cut(s)	4495
BshTI	ACCGGT	1 cut(s)	3578
BshVI	ATCGAT	1 cut(s)	3324
BsiHKAI	GWGCWC	4 cut(s)	484, 1225, 3618, 4723
BsiHKCI	CYCGRG	2 cut(s)	634, 3437
BsiSI	CCGG	8 cut(s)	635, 813, 882, 1109, 1992, 3579, 3658, 3893
BslFI	GGGAC	5 cut(s)	19, 1577, 3963, 4002, 4656
BsmFI	GGGAC	5 cut(s)	19, 1577, 3963, 4002, 4656
BsmI	GAATGC	3 cut(s)	3113, 3508, 4114
Bso31I	GGTCTC	3 cut(s)	1573, 1640, 3569
BsoBI	CYCGRG	2 cut(s)	634, 3437
Bsp119I	TTCGAA	2 cut(s)	3524, 3679
Bsp1286I	GDGCHC	6 cut(s)	484, 1225, 1360, 3055, 3618, 4723
Bsp13I	TCCGGA	1 cut(s)	812
Bsp1407I	TGTACA	3 cut(s)	517, 2506, 4552
Bsp1720I	GCTNAGC	1 cut(s)	741
BspDI	ATCGAT	1 cut(s)	3324
BspEI	TCCGGA	1 cut(s)	812
BspFNI	CGCG	2 cut(s)	5190, 5192
BspLI	GGNNCC	5 cut(s)	811, 817, 945, 1359, 4497
BspMAI	CTGCAG	6 cut(s)	721, 795, 1408, 1900, 3428, 4777
BspMI	ACCTGC	3 cut(s)	724, 1473, 4039
BspOI	GCTAGC	1 cut(s)	2408
BspQI	GCTCTTC	1 cut(s)	4995
BspT104I	TTCGAA	2 cut(s)	3524, 3679
BspT107I	GGYRCC	1 cut(s)	4495
BspTI	CTTAAG	1 cut(s)	2972
BspTNI	GGTCTC	3 cut(s)	1573, 1640, 3569
BsrBI	CCGCTC	1 cut(s)	710
BsrDI	GCAATG	2 cut(s)	1595, 4422
BsrFI	RCCGGY	2 cut(s)	1108, 3578
BsrGI	TGTACA	3 cut(s)	517, 2506, 4552
BssAI	RCCGGY	2 cut(s)	1108, 3578
BssNAI	GTATAC	2 cut(s)	2260, 3930
BssNI	GRCGYC	1 cut(s)	83
BssSI	CACGAG	2 cut(s)	3042, 3719
BssT1I	CCWWGG	3 cut(s)	3633, 4912, 4979
Bst1107I	GTATAC	2 cut(s)	2260, 3930
Bst2BI	CACGAG	2 cut(s)	3042, 3719
Bst4CI	ACNGT	7 cut(s)	405, 1374, 2257, 2516, 2634, 3716, 4827
Bst6I	CTCTTC	5 cut(s)	163, 2226, 3180, 4995, 5079
BstACI	GRCGYC	1 cut(s)	83
BstAFI	CTTAAG	1 cut(s)	2972
BstAPI	GCANNNNNTGC	1 cut(s)	508
BstAUI	TGTACA	3 cut(s)	517, 2506, 4552
BstBAI	YACGTR	2 cut(s)	647, 5051
BstBI	TTCGAA	2 cut(s)	3524, 3679
BstDSI	CCRYGG	1 cut(s)	2599
BstEII	GGTNACC	1 cut(s)	2920
BstENI	CCTNNNNNAGG	1 cut(s)	471
BstFNI	CGCG	2 cut(s)	5190, 5192
BstH2I	RGCGCY	3 cut(s)	419, 3633, 3644
BstHHI	GCGC	8 cut(s)	418, 2106, 3367, 3632, 3643, 3829, 4235, 5192
BstNSI	RCATGY	2 cut(s)	4233, 4536
BstPI	GGTNACC	1 cut(s)	2920
BstUI	CGCG	2 cut(s)	5190, 5192
BstV2I	GAAGAC	1 cut(s)	1530
BstX2I	RGATCY	7 cut(s)	527, 872, 1873, 2068, 2344, 4740, 4795
BstYI	RGATCY	7 cut(s)	527, 872, 1873, 2068, 2344, 4740, 4795
BstZ17I	GTATAC	2 cut(s)	2260, 3930
Bsu15I	ATCGAT	1 cut(s)	3324
BsuI	GTATCC	1 cut(s)	2961
BsuTUI	ATCGAT	1 cut(s)	3324
BtgI	CCRYGG	1 cut(s)	2599
BtrI	CACGTC	1 cut(s)	4646
BtsI	GCAGTG	4 cut(s)	1413, 2283, 3664, 4317
BveI	ACCTGC	3 cut(s)	724, 1473, 4039
CaiI	CAGNNNCTG	3 cut(s)	104, 1193, 2333
CfoI	GCGC	8 cut(s)	418, 2106, 3367, 3632, 3643, 3829, 4235, 5192
Cfr10I	RCCGGY	2 cut(s)	1108, 3578
Cfr9I	CCCGGG	1 cut(s)	634
ClaI	ATCGAT	1 cut(s)	3324
CseI	GACGC	1 cut(s)	91
CsiI	ACCWGGT	2 cut(s)	559, 4699
CspAI	ACCGGT	1 cut(s)	3578
CspCI	CAANNNNNGTGG	2 cut(s)	4404, 4439
DraIII	CACNNNGTG	1 cut(s)	827
DrdI	GACNNNNNNGTC	1 cut(s)	1589
DseDI	GACNNNNNNGTC	1 cut(s)	1589
EaeI	YGGCCR	3 cut(s)	1471, 1798, 3835
Eam1104I	CTCTTC	5 cut(s)	163, 2226, 3180, 4995, 5079
EarI	CTCTTC	5 cut(s)	163, 2226, 3180, 4995, 5079
Ecl136II	GAGCTC	1 cut(s)	482
Eco130I	CCWWGG	3 cut(s)	3633, 4912, 4979
Eco147I	AGGCCT	4 cut(s)	286, 925, 2808, 4548
Eco24I	GRGCYC	3 cut(s)	484, 1360, 3055
Eco31I	GGTCTC	3 cut(s)	1573, 1640, 3569
Eco47I	GGWCC	9 cut(s)	75, 101, 263, 359, 815, 1291, 1709, 2163, 5153
Eco53kI	GAGCTC	1 cut(s)	482
Eco57I	CTGAAG	7 cut(s)	81, 181, 838, 1924, 2570, 4827, 4931
Eco72I	CACGTG	1 cut(s)	647
Eco88I	CYCGRG	2 cut(s)	634, 3437
Eco91I	GGTNACC	1 cut(s)	2920
EcoICRI	GAGCTC	1 cut(s)	482
EcoNI	CCTNNNNNAGG	1 cut(s)	471
EcoO109I	RGGNCCY	2 cut(s)	101, 4909
EcoO65I	GGTNACC	1 cut(s)	2920
EcoRI	GAATTC	1 cut(s)	3473
EcoT14I	CCWWGG	3 cut(s)	3633, 4912, 4979
EcoT38I	GRGCYC	3 cut(s)	484, 1360, 3055
ErhI	CCWWGG	3 cut(s)	3633, 4912, 4979
FalI	AAGNNNNNCTT	8 cut(s)	1713, 1745, 2980, 3012, 3332, 3364, 4772, 4804
FaqI	GGGAC	5 cut(s)	19, 1577, 3963, 4002, 4656
FauI	CCCGC	6 cut(s)	672, 715, 1347, 3187, 3464, 4037
FauNDI	CATATG	3 cut(s)	1162, 1174, 4801
FbaI	TGATCA	2 cut(s)	3150, 4902
FblI	GTMKAC	3 cut(s)	22, 2259, 3929
FriOI	GRGCYC	3 cut(s)	484, 1360, 3055
FspAI	RTGCGCAY	1 cut(s)	2105
FspI	TGCGCA	2 cut(s)	2105, 3828
GlaI	GCGC	8 cut(s)	417, 2105, 3366, 3631, 3642, 3828, 4234, 5191
GsaI	CCCAGC	4 cut(s)	605, 613, 1134, 4491
GsuI	CTGGAG	2 cut(s)	453, 4429
HaeII	RGCGCY	3 cut(s)	419, 3633, 3644
HapII	CCGG	8 cut(s)	635, 813, 882, 1109, 1992, 3579, 3658, 3893
HgaI	GACGC	1 cut(s)	91
HhaI	GCGC	8 cut(s)	418, 2106, 3367, 3632, 3643, 3829, 4235, 5192
Hin1I	GRCGYC	1 cut(s)	83
Hin6I	GCGC	8 cut(s)	416, 2104, 3365, 3630, 3641, 3827, 4233, 5190
HinP1I	GCGC	8 cut(s)	416, 2104, 3365, 3630, 3641, 3827, 4233, 5190
HincII	GTYRAC	6 cut(s)	23, 1618, 1745, 3421, 4315, 5239
HindII	GTYRAC	6 cut(s)	23, 1618, 1745, 3421, 4315, 5239
HindIII	AAGCTT	4 cut(s)	2684, 3621, 4781, 5231
HpaI	GTTAAC	2 cut(s)	3421, 4315
HpaII	CCGG	8 cut(s)	635, 813, 882, 1109, 1992, 3579, 3658, 3893
Hpy99I	CGWCG	2 cut(s)	1394, 3272
HpyCH4III	ACNGT	7 cut(s)	405, 1374, 2257, 2516, 2634, 3716, 4827
HpyCH4IV	ACGT	8 cut(s)	646, 1392, 1584, 1929, 3104, 3754, 4645, 5050
HpySE526I	ACGT	8 cut(s)	646, 1392, 1584, 1929, 3104, 3754, 4645, 5050
Hsp92I	GRCGYC	1 cut(s)	83
HspAI	GCGC	8 cut(s)	416, 2104, 3365, 3630, 3641, 3827, 4233, 5190
Kpn2I	TCCGGA	1 cut(s)	812
KpnI	GGTACC	1 cut(s)	4499
Ksp22I	TGATCA	2 cut(s)	3150, 4902
KspAI	GTTAAC	2 cut(s)	3421, 4315
LguI	GCTCTTC	1 cut(s)	4995
MabI	ACCWGGT	2 cut(s)	559, 4699
MaeII	ACGT	8 cut(s)	646, 1392, 1584, 1929, 3104, 3754, 4645, 5050
MaeIII	GTNAC	9 cut(s)	1177, 1943, 2338, 2386, 2920, 3369, 4507, 4753, 4971
MbiI	CCGCTC	1 cut(s)	710
MfeI	CAATTG	3 cut(s)	888, 4692, 5127
MflI	RGATCY	7 cut(s)	527, 872, 1873, 2068, 2344, 4740, 4795
MhlI	GDGCHC	6 cut(s)	484, 1225, 1360, 3055, 3618, 4723
MlsI	TGGCCA	2 cut(s)	1473, 3837
MluNI	TGGCCA	2 cut(s)	1473, 3837
MmeI	TCCRAC	8 cut(s)	91, 157, 850, 1361, 2415, 3291, 3675, 4638
Mox20I	TGGCCA	2 cut(s)	1473, 3837
MroI	TCCGGA	1 cut(s)	812
MroXI	GAANNNNTTC	1 cut(s)	3491
MscI	TGGCCA	2 cut(s)	1473, 3837
MslI	CAYNNNNRTG	4 cut(s)	1317, 2654, 4028, 4749
Msp20I	TGGCCA	2 cut(s)	1473, 3837
MspA1I	CMGCKG	2 cut(s)	2189, 2906
MspCI	CTTAAG	1 cut(s)	2972
MspI	CCGG	8 cut(s)	635, 813, 882, 1109, 1992, 3579, 3658, 3893
MunI	CAATTG	3 cut(s)	888, 4692, 5127
Mva1269I	GAATGC	3 cut(s)	3113, 3508, 4114
MvnI	CGCG	2 cut(s)	5190, 5192
NciI	CCSGG	5 cut(s)	635, 636, 882, 1993, 3659
NdeI	CATATG	3 cut(s)	1162, 1174, 4801
NheI	GCTAGC	1 cut(s)	2404
NlaIV	GGNNCC	5 cut(s)	811, 817, 945, 1359, 4497
NmeAIII	GCCGAG	1 cut(s)	945
NmuCI	GTSAC	6 cut(s)	1177, 1943, 2386, 2920, 4753, 4971
NsbI	TGCGCA	2 cut(s)	2105, 3828
NspI	RCATGY	2 cut(s)	4233, 4536
NspV	TTCGAA	2 cut(s)	3524, 3679
PaeI	GCATGC	2 cut(s)	4233, 4536
PaqCI	CACCTGC	2 cut(s)	724, 4039
PceI	AGGCCT	4 cut(s)	286, 925, 2808, 4548
PciSI	GCTCTTC	1 cut(s)	4995
PcsI	WCGNNNNNNNCGW	1 cut(s)	3806
PctI	GAATGC	3 cut(s)	3113, 3508, 4114
PdmI	GAANNNNTTC	1 cut(s)	3491
PfeI	GAWTC	9 cut(s)	590, 1085, 1628, 2798, 2881, 3296, 3352, 4199, 5057
PflFI	GACNNNGTC	2 cut(s)	2422, 4010
PflMI	CCANNNNNTGG	3 cut(s)	827, 1790, 3844
PfoI	TCCNGGA	4 cut(s)	469, 880, 1677, 5155
PinAI	ACCGGT	1 cut(s)	3578
PmaCI	CACGTG	1 cut(s)	647
PmlI	CACGTG	1 cut(s)	647
Ppu21I	YACGTR	2 cut(s)	647, 5051
PpuMI	RGGWCCY	1 cut(s)	101
PsiI	TTATAA	2 cut(s)	1527, 4679
Psp124BI	GAGCTC	1 cut(s)	484
Psp1406I	AACGTT	1 cut(s)	3104
Psp5II	RGGWCCY	1 cut(s)	101
PspCI	CACGTG	1 cut(s)	647
PspEI	GGTNACC	1 cut(s)	2920
PspFI	CCCAGC	4 cut(s)	601, 609, 1130, 4487
PspN4I	GGNNCC	5 cut(s)	811, 817, 945, 1359, 4497
PspPPI	RGGWCCY	1 cut(s)	101
PstI	CTGCAG	6 cut(s)	721, 795, 1408, 1900, 3428, 4777
PstNI	CAGNNNCTG	3 cut(s)	104, 1193, 2333
PsuI	RGATCY	7 cut(s)	527, 872, 1873, 2068, 2344, 4740, 4795
PsyI	GACNNNGTC	2 cut(s)	2422, 4010
PvuII	CAGCTG	2 cut(s)	2189, 2906
RseI	CAYNNNNRTG	4 cut(s)	1317, 2654, 4028, 4749
SacI	GAGCTC	1 cut(s)	484
SalI	GTCGAC	1 cut(s)	21
SapI	GCTCTTC	1 cut(s)	4995
ScaI	AGTACT	1 cut(s)	4881
SduI	GDGCHC	6 cut(s)	484, 1225, 1360, 3055, 3618, 4723
SexAI	ACCWGGT	2 cut(s)	559, 4699
SfuI	TTCGAA	2 cut(s)	3524, 3679
SinI	GGWCC	9 cut(s)	75, 101, 263, 359, 815, 1291, 1709, 2163, 5153
SmaI	CCCGGG	1 cut(s)	636
SmiMI	CAYNNNNRTG	4 cut(s)	1317, 2654, 4028, 4749
SmlI	CTYRAG	3 cut(s)	2972, 3992, 5006
SmoI	CTYRAG	3 cut(s)	2972, 3992, 5006
SpeI	ACTAGT	1 cut(s)	2779
SphI	GCATGC	2 cut(s)	4233, 4536
SseBI	AGGCCT	4 cut(s)	286, 925, 2808, 4548
SstI	GAGCTC	1 cut(s)	484
StuI	AGGCCT	4 cut(s)	286, 925, 2808, 4548
StyI	CCWWGG	3 cut(s)	3633, 4912, 4979
TaaI	ACNGT	7 cut(s)	405, 1374, 2257, 2516, 2634, 3716, 4827
TaiI	ACGT	8 cut(s)	649, 1395, 1587, 1932, 3107, 3757, 4648, 5053
TaqII	GACCGA	1 cut(s)	1437
TauI	GCSGC	5 cut(s)	1369, 1803, 2642, 3641, 3657
TfiI	GAWTC	9 cut(s)	590, 1085, 1628, 2798, 2881, 3296, 3352, 4199, 5057
TseFI	GTSAC	6 cut(s)	1177, 1943, 2386, 2920, 4753, 4971
Tsp45I	GTSAC	6 cut(s)	1177, 1943, 2386, 2920, 4753, 4971
TspGWI	ACGGA	2 cut(s)	2588, 4157
TspMI	CCCGGG	1 cut(s)	634
Tth111I	GACNNNGTC	2 cut(s)	2422, 4010
Van91I	CCANNNNNTGG	3 cut(s)	827, 1790, 3844
Vha464I	CTTAAG	1 cut(s)	2972
VpaK11BI	GGWCC	9 cut(s)	75, 101, 263, 359, 815, 1291, 1709, 2163, 5153
XagI	CCTNNNNNAGG	1 cut(s)	471
XbaI	TCTAGA	3 cut(s)	1654, 4181, 5014
XceI	RCATGY	2 cut(s)	4233, 4536
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2372, 5285
XmaI	CCCGGG	1 cut(s)	634
XmiI	GTMKAC	3 cut(s)	22, 2259, 3929
XmnI	GAANNNNTTC	1 cut(s)	3491
ZrmI	AGTACT	1 cut(s)	4881

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.