MD04G1210600.v1.1

Protein FANTASTIC FOUR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014217
Physical Location & Seq
Reverse (-)
29504912 .. 29506105
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1210600.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGTACTACAACTCTCCTCTCCTCCTCCTCACCCTTCTATTCATTTCTCTACTGAATCTATATAAACATAATAAGTTTAGTAATTTTGTAATTGTATTAAATCCAATTATGATGATTAGCTTTTGTAGAAAGATCACCCCTCACTCTATCCTTGGCCTCACCACAGATACTACCCCCACAACAGCAACAACAAAAACCACCTCCGTTGATGCTCATGAAAAGCTCCCATTAAAATGCCACCGTTACATTTCGCCTTTAAGTTCTACAGGATCAGGAGGAGGAGGGTTACTAGGATTGGTCGCCGCAACCGATAACGATCGCATGAAAAGCCAACCCCTTGTTTTGGAATCTTCATCCATCAAATCATCATCATCCTCCTCCTCCGCCTCCTCCTCCTCCTCCTTCTCCTCCTCGTCATCAGACTCGTCACCGCCAGCTTTGTCACCGGTGGTGTTTGAATTTGGAACAAAGAGAGATCCAGGTGGGTTTGGGTTCATAGACAACATAGGAGGTGGTGTGGACGGCTTGATGTCGTGCACGGAAAGTCTAGGGTTTGAAAGCTCGGACGAGAGGCTGGTGGACGATCATCAGATCGTTGAAAGAATTAGCCATGTTCATGAGAAAGCTGATGAGAATGATCAAGATGGTATCGAGGAGGATGCATGTTTGAGGATGATGAGGCACTCGATTAAGAGAGCGCCGAAATGGACGAAGATGGGGGAGAGGAGAGTGGAGCCCAAGAAGTTTCCTCCCCCGCTTTCATCATTGAATCAAAATGGACATCCAAGGTATTATCTGAGGCCTGAGAGGAAAGATGGGAGGTTGGAGCTTACGGAGGTGAGGATTTATAGGCCTGAGATTTTGAGAGCTTATCGTGAAGGTGGACGGTTGAGATTGCATCTTATTAGCCACGAACCTCACATCCAAGAAGAGGAAGAACCAATATTAGAACGCATAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATTTAATGATGAGGAGGAGGAAATAATTAATGAGGAGAAAGAGGAGGAGAGTGTTGGTGAGTGGAAGCTGAAGGTAGGAGGAGAAGGGTTTCGCCGGTGTCAAAATCTGGTGAACCGCCACCATGTCCATCACCACCACCACCACCACAACCACCACGGCAACTTGCCTGTGTGGAGCCAGCAGCAGCATTGCGTGACAACCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAF

Protein Analysis

398

Amino Acids

45.0

Weight (kDa)

5.78

Isoelectric Point (pI)

70.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF PF11250 248 - 301 1.1e-18 Fantastic Four meristem regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014217)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54740
fragaria_vesca FvH4_6g05230
malus_domestica MD04G1210600.v1.1 MD12G1224900.v1.1
prunus_persica Prupe.6G331500_v2.0.a1
pyrus_communis pycom04g18650 pycom12g21030
rosa_chinensis RchiOBHm_Chr3g0453941
rosa_laevigata RLG00000025493
rosa_multiflora Rmu_sc0003556.1_g000030
rosa_roxburghii Rroxscaffold_6G00430780
rosa_rugosa Rorug02G0651900
rosa_samantha Rh3AG054500 Rh3BG056100 Rh3CG055300 Rh3DG056600
rosa_wichuraiana Rw3G004180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 303, 384, 431, 755, 1102
AclWI GGATC 2 cut(s) 277, 470
AcsI RAATTY 2 cut(s) 458, 986
AcuI CTGAAG 1 cut(s) 1076
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 343, 931
AgeI ACCGGT 1 cut(s) 445
AgsI TTSAA 4 cut(s) 458, 557, 599, 769
AjnI CCWGG 2 cut(s) 478, 1187
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
AluBI AGCT 8 cut(s) 120, 223, 437, 561, 626, 829, 869, 1054
AluI AGCT 8 cut(s) 120, 223, 437, 561, 626, 829, 869, 1054
Alw21I GWGCWC 1 cut(s) 539
Alw44I GTGCAC 1 cut(s) 535
AlwI GGATC 2 cut(s) 277, 470
AoxI GGCC 3 cut(s) 154, 800, 851
ApaLI GTGCAC 1 cut(s) 535
ApeKI GCWGC 2 cut(s) 1168, 1171
ApoI RAATTY 2 cut(s) 458, 986
AseI ATTAAT 1 cut(s) 1014
AsiGI ACCGGT 1 cut(s) 445
Asp700I GAANNNNTTC 1 cut(s) 1074
AspLEI GCGC 1 cut(s) 700
AsuHPI GGTGA 9 cut(s) 22, 127, 151, 420, 435, 850, 1055, 1108, 1109
BaeGI GKGCMC 1 cut(s) 539
BanII GRGCYC 1 cut(s) 738
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 2 cut(s) 1180, 1183
BccI CCATC 5 cut(s) 365, 638, 709, 809, 1122
BceAI ACGGC 2 cut(s) 538, 1159
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciT130I CCWGG 2 cut(s) 480, 1189
BclI TGATCA 1 cut(s) 637
BfaI CTAG 2 cut(s) 290, 548
BfmI CTRYAG 1 cut(s) 264
BfoI RGCGCY 1 cut(s) 701
BisI GCNGC 3 cut(s) 303, 1169, 1172
BlsI GCNGC 3 cut(s) 304, 1170, 1173
Bme1390I CCNGG 2 cut(s) 480, 1189
BmiI GGNNCC 2 cut(s) 735, 1163
BmrFI CCNGG 2 cut(s) 480, 1189
BmsI GCATC 3 cut(s) 199, 649, 907
BsaBI GATNNNNATC 1 cut(s) 315
BsaJI CCNNGG 3 cut(s) 151, 785, 1141
BsaWI WCCGGW 1 cut(s) 445
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 931
Bse118I RCCGGY 2 cut(s) 445, 1080
Bse3DI GCAATG 1 cut(s) 1174
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 2 cut(s) 480, 1189
BseDI CCNNGG 3 cut(s) 151, 785, 1141
BseGI GGATG 6 cut(s) 353, 371, 664, 678, 781, 921
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 343, 931
BseMI GCAATG 1 cut(s) 1174
BseMII CTCAG 3 cut(s) 788, 795, 846
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 2 cut(s) 1180, 1183
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 3 cut(s) 156, 802, 853
BshTI ACCGGT 1 cut(s) 445
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 539
BsiSI CCGG 2 cut(s) 446, 1081
BslI CCNNNNNNNGG 2 cut(s) 343, 931
BsnI GGCC 3 cut(s) 156, 802, 853
Bsp1286I GDGCHC 2 cut(s) 539, 738
Bsp143I GATC 7 cut(s) 132, 269, 316, 475, 583, 591, 637
BspACI CCGC 5 cut(s) 303, 384, 431, 755, 1102
BspANI GGCC 3 cut(s) 156, 802, 853
BspCNI CTCAG 3 cut(s) 789, 796, 847
BspHI TCATGA 2 cut(s) 214, 616
BspLI GGNNCC 2 cut(s) 735, 1163
BspPI GGATC 2 cut(s) 277, 470
BsrDI GCAATG 1 cut(s) 1174
BsrFI RCCGGY 2 cut(s) 445, 1080
BssAI RCCGGY 2 cut(s) 445, 1080
BssECI CCNNGG 3 cut(s) 151, 785, 1141
BssMI GATC 7 cut(s) 132, 269, 316, 475, 583, 591, 637
BssT1I CCWWGG 2 cut(s) 151, 785
Bst2UI CCWGG 2 cut(s) 480, 1189
Bst4CI ACNGT 2 cut(s) 242, 888
Bst6I CTCTTC 1 cut(s) 924
BstC8I GCNNGC 2 cut(s) 435, 1166
BstDEI CTNAG 3 cut(s) 797, 804, 855
BstDSI CCRYGG 1 cut(s) 1141
BstF5I GGATG 6 cut(s) 353, 371, 664, 678, 781, 921
BstH2I RGCGCY 1 cut(s) 701
BstHHI GCGC 1 cut(s) 700
BstKTI GATC 7 cut(s) 135, 272, 319, 478, 586, 594, 640
BstMBI GATC 7 cut(s) 132, 269, 316, 475, 583, 591, 637
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstNI CCWGG 2 cut(s) 480, 1189
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 2 cut(s) 478, 1187
BstSFI CTRYAG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 539
BstV1I GCAGC 2 cut(s) 1180, 1183
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
BsuRI GGCC 3 cut(s) 156, 802, 853
BtgI CCRYGG 1 cut(s) 1141
BtsCI GGATG 6 cut(s) 353, 371, 664, 678, 781, 921
Cac8I GCNNGC 2 cut(s) 435, 1166
CciI TCATGA 2 cut(s) 214, 616
CfoI GCGC 1 cut(s) 700
Cfr10I RCCGGY 2 cut(s) 445, 1080
CsiI ACCWGGT 1 cut(s) 1187
Csp6I GTAC 1 cut(s) 4
CspAI ACCGGT 1 cut(s) 445
CspCI CAANNNNNGTGG 2 cut(s) 187, 222
CviAII CATG 6 cut(s) 215, 322, 611, 617, 663, 1109
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 3 cut(s) 797, 804, 855
DpnI GATC 7 cut(s) 134, 271, 318, 477, 585, 593, 639
DpnII GATC 7 cut(s) 132, 269, 316, 475, 583, 591, 637
Eam1104I CTCTTC 1 cut(s) 924
EarI CTCTTC 1 cut(s) 924
EciI GGCGGA 1 cut(s) 373
Eco130I CCWWGG 2 cut(s) 151, 785
Eco147I AGGCCT 2 cut(s) 802, 853
Eco24I GRGCYC 1 cut(s) 738
Eco57I CTGAAG 1 cut(s) 1076
EcoRII CCWGG 2 cut(s) 478, 1187
EcoT14I CCWWGG 2 cut(s) 151, 785
EcoT22I ATGCAT 1 cut(s) 664
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 2 cut(s) 151, 785
FaeI CATG 6 cut(s) 218, 325, 614, 620, 666, 1112
FatI CATG 6 cut(s) 214, 321, 610, 616, 662, 1108
FauI CCCGC 1 cut(s) 762
FbaI TGATCA 1 cut(s) 637
Fnu4HI GCNGC 3 cut(s) 303, 1169, 1172
FokI GGATG 6 cut(s) 340, 358, 671, 685, 768, 908
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 3 cut(s) 303, 1169, 1172
FspBI CTAG 2 cut(s) 290, 548
GlaI GCGC 1 cut(s) 699
GluI GCNGC 3 cut(s) 303, 1169, 1172
HaeII RGCGCY 1 cut(s) 701
HaeIII GGCC 3 cut(s) 156, 802, 853
HapII CCGG 2 cut(s) 446, 1081
HhaI GCGC 1 cut(s) 700
Hin1II CATG 6 cut(s) 218, 325, 614, 620, 666, 1112
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HinfI GANTC 4 cut(s) 55, 347, 422, 769
HpaII CCGG 2 cut(s) 446, 1081
HphI GGTGA 9 cut(s) 22, 127, 151, 420, 435, 850, 1055, 1108, 1109
Hpy166II GTNNAC 5 cut(s) 520, 537, 580, 884, 1099
Hpy188I TCNGA 4 cut(s) 421, 565, 591, 798
Hpy188III TCNNGA 5 cut(s) 215, 273, 617, 641, 875
Hpy8I GTNNAC 5 cut(s) 520, 537, 580, 884, 1099
HpyAV CCTTC 5 cut(s) 44, 412, 872, 1051, 1064
HpyCH4III ACNGT 2 cut(s) 242, 888
HpyCH4V TGCA 3 cut(s) 537, 662, 898
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
HpyF3I CTNAG 3 cut(s) 797, 804, 855
Hsp92II CATG 6 cut(s) 218, 325, 614, 620, 666, 1112
HspAI GCGC 1 cut(s) 698
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 7 cut(s) 132, 269, 316, 475, 583, 591, 637
LmnI GCTCC 4 cut(s) 228, 733, 826, 1161
Lsp1109I GCAGC 2 cut(s) 1180, 1183
LweI GCATC 3 cut(s) 199, 649, 907
MabI ACCWGGT 1 cut(s) 1187
MaeI CTAG 2 cut(s) 290, 548
MaeIII GTNAC 5 cut(s) 242, 285, 426, 441, 1180
MalI GATC 7 cut(s) 134, 271, 318, 477, 585, 593, 639
MboI GATC 7 cut(s) 132, 269, 316, 475, 583, 591, 637
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 2 cut(s) 539, 738
MluCI AATT 7 cut(s) 82, 90, 105, 458, 603, 986, 1011
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 804
Mph1103I ATGCAT 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 1074
MseI TTAA 6 cut(s) 98, 230, 257, 690, 990, 1014
MslI CAYNNNNRTG 2 cut(s) 232, 615
MspI CCGG 2 cut(s) 446, 1081
MspR9I CCNGG 2 cut(s) 480, 1189
MvaI CCWGG 2 cut(s) 480, 1189
MwoI GCNNNNNNNGC 1 cut(s) 327
NdeII GATC 7 cut(s) 132, 269, 316, 475, 583, 591, 637
NlaIII CATG 6 cut(s) 218, 325, 614, 620, 666, 1112
NlaIV GGNNCC 2 cut(s) 735, 1163
NmuCI GTSAC 3 cut(s) 426, 441, 1180
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 1 cut(s) 666
PagI TCATGA 2 cut(s) 214, 616
PceI AGGCCT 2 cut(s) 802, 853
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 1074
PfeI GAWTC 3 cut(s) 55, 347, 769
PinAI ACCGGT 1 cut(s) 445
PkrI GCNGC 3 cut(s) 304, 1170, 1173
Ple19I CGATCG 1 cut(s) 319
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
PshBI ATTAAT 1 cut(s) 1014
Psp6I CCWGG 2 cut(s) 478, 1187
PspGI CCWGG 2 cut(s) 478, 1187
PspN4I GGNNCC 2 cut(s) 735, 1163
PsuI RGATCY 1 cut(s) 475
PvuI CGATCG 1 cut(s) 319
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 232, 615
SaqAI TTAA 6 cut(s) 98, 230, 257, 690, 990, 1014
SatI GCNGC 3 cut(s) 303, 1169, 1172
Sau3AI GATC 7 cut(s) 132, 269, 316, 475, 583, 591, 637
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 2 cut(s) 480, 1189
SduI GDGCHC 2 cut(s) 539, 738
SexAI ACCWGGT 1 cut(s) 1187
SfaNI GCATC 3 cut(s) 199, 649, 907
SfcI CTRYAG 1 cut(s) 264
SgrAI CRCCGGYG 2 cut(s) 445, 1080
SmiMI CAYNNNNRTG 2 cut(s) 232, 615
Sse9I AATT 7 cut(s) 82, 90, 105, 458, 603, 986, 1011
SseBI AGGCCT 2 cut(s) 802, 853
SsiI CCGC 5 cut(s) 303, 384, 431, 755, 1102
SspI AATATT 1 cut(s) 945
SspMI CTAG 2 cut(s) 290, 548
StuI AGGCCT 2 cut(s) 802, 853
StyD4I CCNGG 2 cut(s) 478, 1187
StyI CCWWGG 2 cut(s) 151, 785
TaaI ACNGT 2 cut(s) 242, 888
TaqI TCGA 2 cut(s) 651, 686
TasI AATT 7 cut(s) 82, 90, 105, 458, 603, 986, 1011
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 3 cut(s) 55, 347, 769
Tru1I TTAA 6 cut(s) 98, 230, 257, 690, 990, 1014
Tru9I TTAA 6 cut(s) 98, 230, 257, 690, 990, 1014
TseFI GTSAC 3 cut(s) 426, 441, 1180
TseI GCWGC 2 cut(s) 1168, 1171
Tsp45I GTSAC 3 cut(s) 426, 441, 1180
TspDTI ATGAA 7 cut(s) 31, 231, 338, 342, 484, 605, 750
TspGWI ACGGA 3 cut(s) 193, 554, 848
VneI GTGCAC 1 cut(s) 535
VspI ATTAAT 1 cut(s) 1014
XapI RAATTY 2 cut(s) 458, 986
XceI RCATGY 1 cut(s) 666
XmnI GAANNNNTTC 1 cut(s) 1074
XspI CTAG 2 cut(s) 290, 548
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.