pycom12g21030

Protein FANTASTIC FOUR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014217
Physical Location & Seq
Reverse (-)
22301913 .. 22302927
1015 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21030.1

Sequence Viewer

Length: 963 bp
ATGATGATTAGCTTTTGTAGAAAGATCACTCCTCACTCTATCCTTTGCCTCACCACAACAACTACTACCACAGCCACAACAACAACCTCCATTGATGGTCATGAAAAGCTCCCATTAAAATGTCACCGTTACATTTCTCCTTTAAGTTCTACAAGATCAGGAGGAGGAGGGTTACTGGGGTTGGTTGCTGCAACCGAAAACGATCACAAAAAAAGCCAACCTTTTGTTTTGGAATCTTCATCCATCAAATCCTCCTCGTCATCACCATCAAATTCATCAACACCAGCTTTGTCGCCGGTGGTGTTTGAATTTGGAAAAAAGAAAGATCCAAGAGGGTTTGGGTTCATAGACGACATAGGAGGCGGCGTGGACGGCTTGACGTCGTGCACGGAGAGTCTAGGAATTAGCCATGTTCATGAGAAGGCTGATGAGAATGATCAAGATGGTGTCGAGGAGGATGCATGTTTGAGGATGATGAGGCACTCGATGAAGAGAGCGCCGAAATGGACGAAGATGGGGAAGAGGAGAGTGGAGCCCAAGAAGTATCCACCACCGCTTTCATCGTTGAATCAAAATGGACACCCTAGGTATTATCTGAGGCCTGAGAGGAAAGATGGGAGGTTGGAGCTTACGGAGGTAAGGATTTATAGGCCTGAGATTTTTAGAGCTTATCGTGAAGATGGACGGTTGAGATTGCATCTTGTTAGCCACGAACCTCACATTCAAGAAGAGGAAGAACCAATATTAGAAGCCATAGAAGAAGTACAAGGAGAAGAAGAAGCATTTCTTGATGATGAGGAGGAAATTATTGAGGAGGAGAAAGAGGAAGACAGTGTTGGTGGAGAGTGGCTGAAGGGTGGAGCAGAAGGGTTTCGGCGGTGTCAAAATCTGGTGAACCACCACCACCACCACCGCAACTTGCCTGTGTGGAGCCAGCAGCAGCATTGCGTGACAACCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAF

Protein Analysis

321

Amino Acids

36.13

Weight (kDa)

6.03

Isoelectric Point (pI)

69.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF PF11250 181 - 234 1.8e-19 Fantastic Four meristem regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014217)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54740
fragaria_vesca FvH4_6g05230
malus_domestica MD04G1210600.v1.1 MD12G1224900.v1.1
prunus_persica Prupe.6G331500_v2.0.a1
pyrus_communis pycom04g18650 pycom12g21030
rosa_chinensis RchiOBHm_Chr3g0453941
rosa_laevigata RLG00000025493
rosa_multiflora Rmu_sc0003556.1_g000030
rosa_roxburghii Rroxscaffold_6G00430780
rosa_rugosa Rorug02G0651900
rosa_samantha Rh3AG054500 Rh3BG056100 Rh3CG055300 Rh3DG056600
rosa_wichuraiana Rw3G004180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 383
AciI CCGC 4 cut(s) 363, 554, 877, 913
AclWI GGATC 1 cut(s) 320
AcsI RAATTY 2 cut(s) 271, 308
AcuI CTGAAG 1 cut(s) 872
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 1 cut(s) 765
AgsI TTSAA 3 cut(s) 308, 568, 725
AjnI CCWGG 1 cut(s) 956
AjuI GAANNNNNNNTTGG 2 cut(s) 819, 851
AluBI AGCT 5 cut(s) 12, 109, 287, 628, 668
AluI AGCT 5 cut(s) 12, 109, 287, 628, 668
Alw21I GWGCWC 1 cut(s) 389
Alw44I GTGCAC 1 cut(s) 385
AlwI GGATC 1 cut(s) 320
AoxI GGCC 2 cut(s) 599, 650
ApaLI GTGCAC 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 188, 937, 940
ApoI RAATTY 2 cut(s) 271, 308
Asp700I GAANNNNTTC 2 cut(s) 783, 870
AspA2I CCTAGG 1 cut(s) 584
AspLEI GCGC 1 cut(s) 499
AsuHPI GGTGA 4 cut(s) 43, 116, 255, 904
AvrII CCTAGG 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 537
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 1 cut(s) 389
BbvI GCAGC 3 cut(s) 175, 949, 952
BccI CCATC 7 cut(s) 89, 251, 274, 437, 508, 608, 674
BceAI ACGGC 1 cut(s) 388
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 1 cut(s) 958
BciVI GTATCC 1 cut(s) 555
BclI TGATCA 1 cut(s) 436
BfaI CTAG 2 cut(s) 398, 585
BfoI RGCGCY 1 cut(s) 500
BfuI GTATCC 1 cut(s) 555
BisI GCNGC 4 cut(s) 189, 364, 938, 941
BlnI CCTAGG 1 cut(s) 584
BlsI GCNGC 4 cut(s) 190, 365, 939, 942
Bme1390I CCNGG 1 cut(s) 958
BmiI GGNNCC 2 cut(s) 534, 932
BmrFI CCNGG 1 cut(s) 958
BmrI ACTGGG 1 cut(s) 185
BmsI GCATC 2 cut(s) 448, 706
BmuI ACTGGG 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 834
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 584
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 943
BseBI CCWGG 1 cut(s) 958
BseDI CCNNGG 1 cut(s) 584
BseGI GGATG 3 cut(s) 239, 463, 477
BseMI GCAATG 1 cut(s) 943
BseMII CTCAG 3 cut(s) 587, 594, 645
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 9 cut(s) 21, 177, 180, 244, 467, 538, 812, 827, 830
BseSI GKGCMC 1 cut(s) 389
BseXI GCAGC 3 cut(s) 175, 949, 952
BshFI GGCC 2 cut(s) 601, 652
BsiHKAI GWGCWC 1 cut(s) 389
BsiSI CCGG 1 cut(s) 296
BsnI GGCC 2 cut(s) 601, 652
Bsp1286I GDGCHC 2 cut(s) 389, 537
Bsp143I GATC 5 cut(s) 24, 155, 202, 325, 436
BspACI CCGC 4 cut(s) 363, 554, 877, 913
BspANI GGCC 2 cut(s) 601, 652
BspCNI CTCAG 3 cut(s) 588, 595, 646
BspHI TCATGA 2 cut(s) 100, 415
BspLI GGNNCC 2 cut(s) 534, 932
BspPI GGATC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 943
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 1 cut(s) 180
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 584
BssMI GATC 5 cut(s) 24, 155, 202, 325, 436
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 958
Bst4CI ACNGT 3 cut(s) 128, 687, 833
Bst6I CTCTTC 3 cut(s) 485, 515, 723
BstACI GRCGYC 1 cut(s) 380
BstC8I GCNNGC 1 cut(s) 935
BstDEI CTNAG 3 cut(s) 596, 603, 654
BstF5I GGATG 3 cut(s) 239, 463, 477
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 1 cut(s) 499
BstKTI GATC 5 cut(s) 27, 158, 205, 328, 439
BstMBI GATC 5 cut(s) 24, 155, 202, 325, 436
BstMWI GCNNNNNNNGC 1 cut(s) 372
BstNI CCWGG 1 cut(s) 958
BstNSI RCATGY 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 956
BstSLI GKGCMC 1 cut(s) 389
BstV1I GCAGC 3 cut(s) 175, 949, 952
BstV2I GAAGAC 1 cut(s) 834
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 2 cut(s) 601, 652
BtsCI GGATG 3 cut(s) 239, 463, 477
BtsIMutI CAGTG 1 cut(s) 838
Cac8I GCNNGC 1 cut(s) 935
CciI TCATGA 2 cut(s) 100, 415
CfoI GCGC 1 cut(s) 499
Cfr10I RCCGGY 1 cut(s) 295
CsiI ACCWGGT 1 cut(s) 956
Csp6I GTAC 1 cut(s) 764
CviAII CATG 4 cut(s) 101, 410, 416, 462
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 3 cut(s) 596, 603, 654
DpnI GATC 5 cut(s) 26, 157, 204, 327, 438
DpnII GATC 5 cut(s) 24, 155, 202, 325, 436
Eam1104I CTCTTC 3 cut(s) 485, 515, 723
EarI CTCTTC 3 cut(s) 485, 515, 723
Eco130I CCWWGG 1 cut(s) 584
Eco147I AGGCCT 2 cut(s) 601, 652
Eco24I GRGCYC 1 cut(s) 537
Eco57I CTGAAG 1 cut(s) 872
EcoRII CCWGG 1 cut(s) 956
EcoT14I CCWWGG 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 584
FaeI CATG 4 cut(s) 104, 413, 419, 465
FaiI YATR 8 cut(s) 102, 347, 356, 411, 417, 463, 648, 755
FalI AAGNNNNNCTT 4 cut(s) 205, 237, 771, 803
FatI CATG 4 cut(s) 100, 409, 415, 461
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 4 cut(s) 189, 364, 938, 941
FokI GGATG 3 cut(s) 226, 470, 484
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 4 cut(s) 189, 364, 938, 941
FspBI CTAG 2 cut(s) 398, 585
GlaI GCGC 1 cut(s) 498
GluI GCNGC 4 cut(s) 189, 364, 938, 941
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 2 cut(s) 601, 652
HapII CCGG 1 cut(s) 296
HhaI GCGC 1 cut(s) 499
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 4 cut(s) 104, 413, 419, 465
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HinfI GANTC 3 cut(s) 233, 394, 568
HpaII CCGG 1 cut(s) 296
HphI GGTGA 4 cut(s) 43, 116, 255, 904
Hpy166II GTNNAC 3 cut(s) 370, 387, 895
Hpy188I TCNGA 1 cut(s) 597
Hpy188III TCNNGA 7 cut(s) 101, 159, 416, 440, 674, 725, 788
Hpy8I GTNNAC 3 cut(s) 370, 387, 895
Hpy99I CGWCG 1 cut(s) 385
HpyAV CCTTC 3 cut(s) 415, 847, 860
HpyCH4III ACNGT 3 cut(s) 128, 687, 833
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 4 cut(s) 191, 387, 461, 697
HpyF10VI GCNNNNNNNGC 1 cut(s) 372
HpyF3I CTNAG 3 cut(s) 596, 603, 654
HpySE526I ACGT 1 cut(s) 380
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 4 cut(s) 104, 413, 419, 465
HspAI GCGC 1 cut(s) 497
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 5 cut(s) 24, 155, 202, 325, 436
LmnI GCTCC 5 cut(s) 114, 532, 625, 860, 930
Lsp1109I GCAGC 3 cut(s) 175, 949, 952
LweI GCATC 2 cut(s) 448, 706
MabI ACCWGGT 1 cut(s) 956
MaeI CTAG 2 cut(s) 398, 585
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 4 cut(s) 122, 128, 171, 949
MalI GATC 5 cut(s) 26, 157, 204, 327, 438
MboI GATC 5 cut(s) 24, 155, 202, 325, 436
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 2 cut(s) 389, 537
MluCI AATT 4 cut(s) 271, 308, 402, 804
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 603
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 2 cut(s) 783, 870
MseI TTAA 2 cut(s) 116, 143
MslI CAYNNNNRTG 2 cut(s) 118, 414
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 958
MvaI CCWGG 1 cut(s) 958
MwoI GCNNNNNNNGC 1 cut(s) 372
NdeII GATC 5 cut(s) 24, 155, 202, 325, 436
NlaIII CATG 4 cut(s) 104, 413, 419, 465
NlaIV GGNNCC 2 cut(s) 534, 932
NmuCI GTSAC 2 cut(s) 122, 949
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 1 cut(s) 465
PagI TCATGA 2 cut(s) 100, 415
PceI AGGCCT 2 cut(s) 601, 652
PdmI GAANNNNTTC 2 cut(s) 783, 870
PfeI GAWTC 2 cut(s) 233, 568
PkrI GCNGC 4 cut(s) 190, 365, 939, 942
PleI GAGTC 1 cut(s) 402
PpsI GAGTC 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 956
PspGI CCWGG 1 cut(s) 956
PspN4I GGNNCC 2 cut(s) 534, 932
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
RseI CAYNNNNRTG 2 cut(s) 118, 414
SaqAI TTAA 2 cut(s) 116, 143
SatI GCNGC 4 cut(s) 189, 364, 938, 941
Sau3AI GATC 5 cut(s) 24, 155, 202, 325, 436
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 1 cut(s) 958
SduI GDGCHC 2 cut(s) 389, 537
SexAI ACCWGGT 1 cut(s) 956
SfaNI GCATC 2 cut(s) 448, 706
SgrAI CRCCGGYG 1 cut(s) 295
SmiMI CAYNNNNRTG 2 cut(s) 118, 414
Sse9I AATT 4 cut(s) 271, 308, 402, 804
SseBI AGGCCT 2 cut(s) 601, 652
SsiI CCGC 4 cut(s) 363, 554, 877, 913
SspI AATATT 1 cut(s) 744
SspMI CTAG 2 cut(s) 398, 585
StuI AGGCCT 2 cut(s) 601, 652
StyD4I CCNGG 1 cut(s) 956
StyI CCWWGG 1 cut(s) 584
TaaI ACNGT 3 cut(s) 128, 687, 833
TaiI ACGT 1 cut(s) 383
TaqI TCGA 2 cut(s) 450, 485
TasI AATT 4 cut(s) 271, 308, 402, 804
TatI WGTACW 1 cut(s) 763
TauI GCSGC 1 cut(s) 366
TfiI GAWTC 2 cut(s) 233, 568
Tru1I TTAA 2 cut(s) 116, 143
Tru9I TTAA 2 cut(s) 116, 143
TscAI CASTG 1 cut(s) 838
TseFI GTSAC 2 cut(s) 122, 949
TseI GCWGC 3 cut(s) 188, 937, 940
Tsp45I GTSAC 2 cut(s) 122, 949
TspDTI ATGAA 7 cut(s) 117, 228, 264, 334, 404, 503, 549
TspGWI ACGGA 2 cut(s) 404, 647
TspRI CASTG 1 cut(s) 838
VneI GTGCAC 1 cut(s) 385
XapI RAATTY 2 cut(s) 271, 308
XceI RCATGY 1 cut(s) 465
XmaJI CCTAGG 1 cut(s) 584
XmnI GAANNNNTTC 2 cut(s) 783, 870
XspI CTAG 2 cut(s) 398, 585
ZraI GACGTC 1 cut(s) 381
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.