MD12G1224900.v1.1

Protein FANTASTIC FOUR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014217
Physical Location & Seq
Reverse (-)
30079360 .. 30080544
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1224900.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGTGCTACTACTACTGCTGTCCTCTCCTCCTCCTCCTCCTCCTCATGCTTCTATTCATTTCTCTATTGAATCTATATAAAAATAATAAGTTTAGTAATATTATAATTGTTTTAAATCCAATTATGATGATTAGCTTTTGTAAAAAGATCACTCCTCACTCTATCCTTGGCCTCACCACAAAAACTACTACCACCGCCACAACAACAACCTCCATTGATGGTCATGAAAAGCTCCCATTAAAATGTCACCATTACATTTCTCCTTTAAGTTCTACAAGATCAGGAGGAGGAGGTTTACTAGGTTTGGTTGCTGCAACCGAAAACGATCACAAAAAAAGCCAACCCCTTGTTTTGGAATCTTCATCCATCAAATCCTCCTCGTCCGCAGCCTCCTCCTCGTCATCACCATCAGATTCATCAACACCAGCTTTGTCGCCGGTGGTGTTTGAATTTGGAAAAAAGAAAGATCCAGGTGGGTTTGGGTTCATAGACGACATAGGAGGCGGCGTGGACGGCTTGACGTCGTGCACGGAGAGTCTAGGGTTTGAAAGTTCAAACGAGAGGCTGGTGGACGATCATCAGATCATTGAAAGAATTAGCCATGTTCATGAGAAGGCTAATGAGAATGATCAAGATGGTGTCGAGGAGGATGCATGTTTGAGGATGATGAGGCACTCGATGAAGAGAGAGCCGAAATGGACGAAGATGGGGAAGAGGAGAGTGGAGCCCAAGAAGTTTCCTCCACCGCTTTCATCGTTGAATCAAAATGGACACCCTAGGTATTATCTGAGGCCTGAGAGGAAAGATGGGAGGTTGGAGCTTACGGAGGTAAGGATTTATAGGCCTGAGATTTTTAGAGCTTATCGTGAAGATGGACGGTTGAGATTGCTTCTTGTTAGCCACGAACCTCACATTCAAGAAGATGAAGAACCAATATTAGAAGGCATAGAAGAAGTACAAGGAGAAGAAAAAGCATTTCTTGATGATGAGGAGGAAATTATTGAGGAGGAGGAGAAGGAGGAGGAGAAAGAGGAAGACAGTGGTGGAGAGTGGCTGAAGGGAGGAGCAGAAGGGTTTCGGCGGTGTCAAAATCTGGTGAACCACCACCATGTCCATCACCACCACCACCACGACGGCAACTTGCCTGTGTGGAGCCAGCAGCAGCATTGCGTGACAACCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAF

Protein Analysis

395

Amino Acids

44.54

Weight (kDa)

5.82

Isoelectric Point (pI)

66.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF PF11250 245 - 298 9.9e-19 Fantastic Four meristem regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014217)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54740
fragaria_vesca FvH4_6g05230
malus_domestica MD04G1210600.v1.1 MD12G1224900.v1.1
prunus_persica Prupe.6G331500_v2.0.a1
pyrus_communis pycom04g18650 pycom12g21030
rosa_chinensis RchiOBHm_Chr3g0453941
rosa_laevigata RLG00000025493
rosa_multiflora Rmu_sc0003556.1_g000030
rosa_roxburghii Rroxscaffold_6G00430780
rosa_rugosa Rorug02G0651900
rosa_samantha Rh3AG054500 Rh3BG056100 Rh3CG055300 Rh3DG056600
rosa_wichuraiana Rw3G004180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 104
AatII GACGTC 1 cut(s) 524
AciI CCGC 5 cut(s) 195, 384, 504, 746, 1081
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 1 cut(s) 449
AcuI CTGAAG 1 cut(s) 1076
AcyI GRCGYC 1 cut(s) 521
AfaI GTAC 1 cut(s) 957
AfiI CCNNNNNNNGG 1 cut(s) 352
AgsI TTSAA 7 cut(s) 70, 449, 548, 555, 590, 760, 917
AjnI CCWGG 2 cut(s) 469, 1178
AluBI AGCT 5 cut(s) 135, 232, 428, 820, 860
AluI AGCT 5 cut(s) 135, 232, 428, 820, 860
Alw21I GWGCWC 1 cut(s) 530
Alw44I GTGCAC 1 cut(s) 526
AlwI GGATC 1 cut(s) 461
AoxI GGCC 3 cut(s) 169, 791, 842
ApaLI GTGCAC 1 cut(s) 526
ApeKI GCWGC 4 cut(s) 311, 386, 1159, 1162
ApoI RAATTY 1 cut(s) 449
Asp700I GAANNNNTTC 1 cut(s) 1074
AspA2I CCTAGG 1 cut(s) 776
AsuHPI GGTGA 5 cut(s) 166, 239, 396, 1108, 1109
AvrII CCTAGG 1 cut(s) 776
BaeGI GKGCMC 1 cut(s) 530
BanII GRGCYC 1 cut(s) 729
BbsI GAAGAC 1 cut(s) 1041
Bbv12I GWGCWC 1 cut(s) 530
BbvI GCAGC 4 cut(s) 298, 398, 1171, 1174
BccI CCATC 8 cut(s) 212, 374, 415, 629, 700, 800, 866, 1122
BceAI ACGGC 2 cut(s) 529, 1150
BcgI CGANNNNNNTGC 2 cut(s) 632, 666
BciT130I CCWGG 2 cut(s) 471, 1180
BclI TGATCA 1 cut(s) 628
BfaI CTAG 3 cut(s) 299, 539, 777
BisI GCNGC 5 cut(s) 312, 387, 505, 1160, 1163
BlnI CCTAGG 1 cut(s) 776
BlsI GCNGC 5 cut(s) 313, 388, 506, 1161, 1164
Bme1390I CCNGG 2 cut(s) 471, 1180
BmiI GGNNCC 2 cut(s) 726, 1154
BmrFI CCNGG 2 cut(s) 471, 1180
BmsI GCATC 1 cut(s) 640
BpiI GAAGAC 1 cut(s) 1041
BsaHI GRCGYC 1 cut(s) 521
BsaJI CCNNGG 2 cut(s) 166, 776
BsaXI ACNNNNNCTCC 2 cut(s) 492, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse118I RCCGGY 1 cut(s) 436
Bse3DI GCAATG 1 cut(s) 1165
BseBI CCWGG 2 cut(s) 471, 1180
BseDI CCNNGG 2 cut(s) 166, 776
BseGI GGATG 3 cut(s) 362, 655, 669
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 1165
BseMII CTCAG 3 cut(s) 779, 786, 837
BseSI GKGCMC 1 cut(s) 530
BseXI GCAGC 4 cut(s) 298, 398, 1171, 1174
BshFI GGCC 3 cut(s) 171, 793, 844
BsiHKAI GWGCWC 1 cut(s) 530
BsiSI CCGG 1 cut(s) 437
BslI CCNNNNNNNGG 1 cut(s) 352
BsnI GGCC 3 cut(s) 171, 793, 844
Bsp1286I GDGCHC 2 cut(s) 530, 729
Bsp143I GATC 7 cut(s) 147, 278, 325, 466, 574, 582, 628
BspACI CCGC 5 cut(s) 195, 384, 504, 746, 1081
BspANI GGCC 3 cut(s) 171, 793, 844
BspCNI CTCAG 3 cut(s) 780, 787, 838
BspHI TCATGA 2 cut(s) 223, 607
BspLI GGNNCC 2 cut(s) 726, 1154
BspPI GGATC 1 cut(s) 461
BsrDI GCAATG 1 cut(s) 1165
BsrFI RCCGGY 1 cut(s) 436
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 2 cut(s) 166, 776
BssMI GATC 7 cut(s) 147, 278, 325, 466, 574, 582, 628
BssNI GRCGYC 1 cut(s) 521
BssT1I CCWWGG 2 cut(s) 166, 776
Bst2UI CCWGG 2 cut(s) 471, 1180
Bst4CI ACNGT 2 cut(s) 879, 1040
Bst6I CTCTTC 2 cut(s) 677, 707
BstACI GRCGYC 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 1157
BstDEI CTNAG 3 cut(s) 788, 795, 846
BstF5I GGATG 3 cut(s) 362, 655, 669
BstKTI GATC 7 cut(s) 150, 281, 328, 469, 577, 585, 631
BstMBI GATC 7 cut(s) 147, 278, 325, 466, 574, 582, 628
BstMWI GCNNNNNNNGC 1 cut(s) 513
BstNI CCWGG 2 cut(s) 471, 1180
BstNSI RCATGY 1 cut(s) 657
BstSCI CCNGG 2 cut(s) 469, 1178
BstSLI GKGCMC 1 cut(s) 530
BstV1I GCAGC 4 cut(s) 298, 398, 1171, 1174
BstV2I GAAGAC 1 cut(s) 1041
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 3 cut(s) 171, 793, 844
BtsCI GGATG 3 cut(s) 362, 655, 669
BtsIMutI CAGTG 1 cut(s) 1045
Cac8I GCNNGC 1 cut(s) 1157
CciI TCATGA 2 cut(s) 223, 607
Cfr10I RCCGGY 1 cut(s) 436
CsiI ACCWGGT 1 cut(s) 1178
Csp6I GTAC 1 cut(s) 956
CviAII CATG 6 cut(s) 46, 224, 602, 608, 654, 1109
CviQI GTAC 1 cut(s) 956
DdeI CTNAG 3 cut(s) 788, 795, 846
DpnI GATC 7 cut(s) 149, 280, 327, 468, 576, 584, 630
DpnII GATC 7 cut(s) 147, 278, 325, 466, 574, 582, 628
DraI TTTAAA 1 cut(s) 114
Eam1104I CTCTTC 2 cut(s) 677, 707
EarI CTCTTC 2 cut(s) 677, 707
Eco130I CCWWGG 2 cut(s) 166, 776
Eco147I AGGCCT 2 cut(s) 793, 844
Eco24I GRGCYC 1 cut(s) 729
Eco57I CTGAAG 1 cut(s) 1076
EcoRII CCWGG 2 cut(s) 469, 1178
EcoT14I CCWWGG 2 cut(s) 166, 776
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 729
ErhI CCWWGG 2 cut(s) 166, 776
FaeI CATG 6 cut(s) 49, 227, 605, 611, 657, 1112
FalI AAGNNNNNCTT 2 cut(s) 963, 995
FatI CATG 6 cut(s) 45, 223, 601, 607, 653, 1108
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 5 cut(s) 312, 387, 505, 1160, 1163
FokI GGATG 3 cut(s) 349, 662, 676
FriOI GRGCYC 1 cut(s) 729
Fsp4HI GCNGC 5 cut(s) 312, 387, 505, 1160, 1163
FspBI CTAG 3 cut(s) 299, 539, 777
GluI GCNGC 5 cut(s) 312, 387, 505, 1160, 1163
HaeIII GGCC 3 cut(s) 171, 793, 844
HapII CCGG 1 cut(s) 437
Hin1I GRCGYC 1 cut(s) 521
Hin1II CATG 6 cut(s) 49, 227, 605, 611, 657, 1112
HinfI GANTC 5 cut(s) 70, 356, 413, 535, 760
HpaII CCGG 1 cut(s) 437
HphI GGTGA 5 cut(s) 166, 239, 396, 1108, 1109
Hpy166II GTNNAC 5 cut(s) 296, 511, 528, 571, 1099
Hpy188I TCNGA 3 cut(s) 412, 582, 789
Hpy188III TCNNGA 7 cut(s) 224, 282, 608, 632, 866, 917, 980
Hpy8I GTNNAC 5 cut(s) 296, 511, 528, 571, 1099
Hpy99I CGWCG 2 cut(s) 526, 1136
HpyAV CCTTC 5 cut(s) 607, 935, 1009, 1051, 1064
HpyCH4III ACNGT 2 cut(s) 879, 1040
HpyCH4IV ACGT 1 cut(s) 521
HpyCH4V TGCA 3 cut(s) 314, 528, 653
HpyF10VI GCNNNNNNNGC 1 cut(s) 513
HpyF3I CTNAG 3 cut(s) 788, 795, 846
HpySE526I ACGT 1 cut(s) 521
Hsp92I GRCGYC 1 cut(s) 521
Hsp92II CATG 6 cut(s) 49, 227, 605, 611, 657, 1112
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 7 cut(s) 147, 278, 325, 466, 574, 582, 628
LmnI GCTCC 5 cut(s) 237, 724, 817, 1064, 1152
Lsp1109I GCAGC 4 cut(s) 298, 398, 1171, 1174
LweI GCATC 1 cut(s) 640
MabI ACCWGGT 1 cut(s) 1178
MaeI CTAG 3 cut(s) 299, 539, 777
MaeII ACGT 1 cut(s) 521
MaeIII GTNAC 2 cut(s) 245, 1171
MalI GATC 7 cut(s) 149, 280, 327, 468, 576, 584, 630
MboI GATC 7 cut(s) 147, 278, 325, 466, 574, 582, 628
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 2 cut(s) 530, 729
MluCI AATT 5 cut(s) 105, 120, 449, 594, 996
MlyI GAGTC 1 cut(s) 544
MmeI TCCRAC 1 cut(s) 795
Mph1103I ATGCAT 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 1074
MseI TTAA 3 cut(s) 113, 239, 266
MslI CAYNNNNRTG 3 cut(s) 241, 606, 1107
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 471, 1180
MvaI CCWGG 2 cut(s) 471, 1180
MwoI GCNNNNNNNGC 1 cut(s) 513
NdeII GATC 7 cut(s) 147, 278, 325, 466, 574, 582, 628
NlaIII CATG 6 cut(s) 49, 227, 605, 611, 657, 1112
NlaIV GGNNCC 2 cut(s) 726, 1154
NmuCI GTSAC 2 cut(s) 245, 1171
NsiI ATGCAT 1 cut(s) 655
NspI RCATGY 1 cut(s) 657
PagI TCATGA 2 cut(s) 223, 607
PceI AGGCCT 2 cut(s) 793, 844
PdmI GAANNNNTTC 1 cut(s) 1074
PfeI GAWTC 4 cut(s) 70, 356, 413, 760
PkrI GCNGC 5 cut(s) 313, 388, 506, 1161, 1164
PleI GAGTC 1 cut(s) 543
PpsI GAGTC 1 cut(s) 543
PsiI TTATAA 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 469, 1178
PspGI CCWGG 2 cut(s) 469, 1178
PspN4I GGNNCC 2 cut(s) 726, 1154
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 1 cut(s) 957
RsaNI GTAC 1 cut(s) 956
RseI CAYNNNNRTG 3 cut(s) 241, 606, 1107
SaqAI TTAA 3 cut(s) 113, 239, 266
SatI GCNGC 5 cut(s) 312, 387, 505, 1160, 1163
Sau3AI GATC 7 cut(s) 147, 278, 325, 466, 574, 582, 628
SchI GAGTC 1 cut(s) 544
ScrFI CCNGG 2 cut(s) 471, 1180
SduI GDGCHC 2 cut(s) 530, 729
SexAI ACCWGGT 1 cut(s) 1178
SfaNI GCATC 1 cut(s) 640
SgrAI CRCCGGYG 1 cut(s) 436
SmiMI CAYNNNNRTG 3 cut(s) 241, 606, 1107
Sse9I AATT 5 cut(s) 105, 120, 449, 594, 996
SseBI AGGCCT 2 cut(s) 793, 844
SsiI CCGC 5 cut(s) 195, 384, 504, 746, 1081
SspI AATATT 2 cut(s) 100, 936
SspMI CTAG 3 cut(s) 299, 539, 777
StuI AGGCCT 2 cut(s) 793, 844
StyD4I CCNGG 2 cut(s) 469, 1178
StyI CCWWGG 2 cut(s) 166, 776
TaaI ACNGT 2 cut(s) 879, 1040
TaiI ACGT 1 cut(s) 524
TaqI TCGA 2 cut(s) 642, 677
TasI AATT 5 cut(s) 105, 120, 449, 594, 996
TatI WGTACW 1 cut(s) 955
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 4 cut(s) 70, 356, 413, 760
Tru1I TTAA 3 cut(s) 113, 239, 266
Tru9I TTAA 3 cut(s) 113, 239, 266
TscAI CASTG 1 cut(s) 1045
TseFI GTSAC 2 cut(s) 245, 1171
TseI GCWGC 4 cut(s) 311, 386, 1159, 1162
Tsp45I GTSAC 2 cut(s) 245, 1171
TspDTI ATGAA 9 cut(s) 46, 240, 351, 405, 475, 596, 695, 741, 939
TspGWI ACGGA 2 cut(s) 545, 839
TspRI CASTG 1 cut(s) 1045
VneI GTGCAC 1 cut(s) 526
XapI RAATTY 1 cut(s) 449
XceI RCATGY 1 cut(s) 657
XmaJI CCTAGG 1 cut(s) 776
XmnI GAANNNNTTC 1 cut(s) 1074
XspI CTAG 3 cut(s) 299, 539, 777
ZraI GACGTC 1 cut(s) 522
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.