pycom04g18650

Protein FANTASTIC FOUR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0014217
Physical Location & Seq
Reverse (-)
20613014 .. 20614091
1078 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18650.1

Sequence Viewer

Length: 1026 bp
ATGATGATTAGCTTTTGTAGAAAGATCACCCCTCACTCTATCCTTGGCCTCACCACAGATACTACCCCCACAACAGCAACAACAAAACCCACCTCCGTTGATGCTCATGAAAAGCTCCCATTAAAATGCCACCATTACATTTCGCCTTTAAGTTCTACAGGATCAGGAGGAGGAGGGTTACTAGGATTGGTCGCCGCAACCGATAACGATCGCATGAAAAGCCAACCCCTTGTTTTGGAATCTTCATCCATCAAATCATCATCCTCCTCCTCCGCCTCCCCTTCCTCCTCCTCCTCGTCATCAGATTCGTCACCGCCAGCTTTGTCGCCGGTGGTGTTTGAATTTGGAACAAAGAGAGATCCAGGTGGGTTTGGGTTCATAGACGACATAGGAGGTGCTGTGGACGGCTTGATGTCGTGCACGGAGAGTCTAGGAATTAGCCATGTTCCTGAGAAAGTTGATGAGAATGATCAAGATGGTATCGAGGAGGATGCATGTTTGAGGATGATGAGGCACTCGATTAAGAGAGCGCCGAAATGGACGAGGATGGGGGAGAGGAGAGTGGAGCCCAAGAAGTTTCCTCCCCCGCTTTCATCATTGAACCAAAATGGACATCCAAGGTATTATCTGAGGCCTGAGAGGAAAGATGGGAGGTTGGAGCTTACGGAGGTAAAGATTTATAGACCTGAGATTTTGAGAGCTTATCGTGAAGGTGGACGGTTGAGATTGAATCTTGTTAGCCACGAACCTCACATCCATGAAAAGGAAGAACCAATATTAGAAGGCATAGAAGAAGTACAAGAAGAAGAAGAAGAAGAGGAAGAATTTATTGATGAGGAGGAGGAAATAATTAGTGAGGAGAAAGAGGAGGAGAGTGTTGGTGAGTGGAAGCTGAAGGTAGGAGGAGAAGGGTGTCGCCGGTGTCAAAATCTGGTGAACCGCCACCATGTCCATCACCATCACCACAACCATCACGGCAACTTGCCTATGTGGAGCCAGCACCAGCATTGCGTGACAACCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAF

Protein Analysis

342

Amino Acids

38.15

Weight (kDa)

5.71

Isoelectric Point (pI)

71.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF PF11250 192 - 245 1e-18 Fantastic Four meristem regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014217)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54740
fragaria_vesca FvH4_6g05230
malus_domestica MD04G1210600.v1.1 MD12G1224900.v1.1
prunus_persica Prupe.6G331500_v2.0.a1
pyrus_communis pycom04g18650 pycom12g21030
rosa_chinensis RchiOBHm_Chr3g0453941
rosa_laevigata RLG00000025493
rosa_multiflora Rmu_sc0003556.1_g000030
rosa_roxburghii Rroxscaffold_6G00430780
rosa_rugosa Rorug02G0651900
rosa_samantha Rh3AG054500 Rh3BG056100 Rh3CG055300 Rh3DG056600
rosa_wichuraiana Rw3G004180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 195, 273, 314, 587, 940
AclWI GGATC 2 cut(s) 169, 353
AcsI RAATTY 2 cut(s) 341, 824
AcuI CTGAAG 1 cut(s) 914
AfaI GTAC 1 cut(s) 798
AfiI CCNNNNNNNGG 2 cut(s) 235, 763
AgsI TTSAA 3 cut(s) 341, 601, 730
AjnI CCWGG 2 cut(s) 361, 1019
AloI GAACNNNNNNTCC 2 cut(s) 738, 770
AluBI AGCT 6 cut(s) 12, 115, 320, 661, 701, 892
AluI AGCT 6 cut(s) 12, 115, 320, 661, 701, 892
Alw21I GWGCWC 1 cut(s) 422
Alw44I GTGCAC 1 cut(s) 418
AlwI GGATC 2 cut(s) 169, 353
AoxI GGCC 2 cut(s) 46, 632
ApaLI GTGCAC 1 cut(s) 418
ApoI RAATTY 2 cut(s) 341, 824
AspLEI GCGC 1 cut(s) 532
AsuHPI GGTGA 7 cut(s) 19, 43, 303, 893, 946, 947, 953
BaeGI GKGCMC 1 cut(s) 422
BanII GRGCYC 1 cut(s) 570
Bbv12I GWGCWC 1 cut(s) 422
BccI CCATC 7 cut(s) 257, 470, 541, 641, 960, 966, 978
BceAI ACGGC 2 cut(s) 421, 991
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 2 cut(s) 363, 1021
BclI TGATCA 1 cut(s) 469
BfaI CTAG 2 cut(s) 182, 431
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 533
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
Bme1390I CCNGG 2 cut(s) 363, 1021
BmiI GGNNCC 2 cut(s) 567, 995
BmrFI CCNGG 2 cut(s) 363, 1021
BmsI GCATC 2 cut(s) 91, 481
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 43, 617
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 763
Bse118I RCCGGY 2 cut(s) 328, 918
Bse3DI GCAATG 1 cut(s) 1006
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 2 cut(s) 363, 1021
BseDI CCNNGG 2 cut(s) 43, 617
BseGI GGATG 7 cut(s) 245, 260, 496, 510, 552, 613, 753
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 2 cut(s) 235, 763
BseMI GCAATG 1 cut(s) 1006
BseMII CTCAG 4 cut(s) 441, 620, 627, 678
BseSI GKGCMC 1 cut(s) 422
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 2 cut(s) 48, 634
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 422
BsiSI CCGG 2 cut(s) 329, 919
BslI CCNNNNNNNGG 2 cut(s) 235, 763
BsnI GGCC 2 cut(s) 48, 634
Bsp1286I GDGCHC 2 cut(s) 422, 570
Bsp143I GATC 5 cut(s) 24, 161, 208, 358, 469
BspACI CCGC 5 cut(s) 195, 273, 314, 587, 940
BspANI GGCC 2 cut(s) 48, 634
BspCNI CTCAG 4 cut(s) 442, 621, 628, 679
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 567, 995
BspPI GGATC 2 cut(s) 169, 353
BsrDI GCAATG 1 cut(s) 1006
BsrFI RCCGGY 2 cut(s) 328, 918
BssAI RCCGGY 2 cut(s) 328, 918
BssECI CCNNGG 2 cut(s) 43, 617
BssMI GATC 5 cut(s) 24, 161, 208, 358, 469
BssT1I CCWWGG 2 cut(s) 43, 617
Bst2UI CCWGG 2 cut(s) 363, 1021
Bst4CI ACNGT 1 cut(s) 720
Bst6I CTCTTC 1 cut(s) 810
BstC8I GCNNGC 2 cut(s) 318, 998
BstDEI CTNAG 4 cut(s) 450, 629, 636, 687
BstF5I GGATG 7 cut(s) 245, 260, 496, 510, 552, 613, 753
BstH2I RGCGCY 1 cut(s) 533
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 5 cut(s) 27, 164, 211, 361, 472
BstMBI GATC 5 cut(s) 24, 161, 208, 358, 469
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstNI CCWGG 2 cut(s) 363, 1021
BstNSI RCATGY 1 cut(s) 498
BstSCI CCNGG 2 cut(s) 361, 1019
BstSFI CTRYAG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 422
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 2 cut(s) 48, 634
BtsCI GGATG 7 cut(s) 245, 260, 496, 510, 552, 613, 753
Cac8I GCNNGC 2 cut(s) 318, 998
CciI TCATGA 1 cut(s) 106
CfoI GCGC 1 cut(s) 532
Cfr10I RCCGGY 2 cut(s) 328, 918
CsiI ACCWGGT 1 cut(s) 1019
Csp6I GTAC 1 cut(s) 797
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviAII CATG 6 cut(s) 107, 214, 443, 495, 758, 947
CviQI GTAC 1 cut(s) 797
DdeI CTNAG 4 cut(s) 450, 629, 636, 687
DpnI GATC 5 cut(s) 26, 163, 210, 360, 471
DpnII GATC 5 cut(s) 24, 161, 208, 358, 469
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
EciI GGCGGA 1 cut(s) 262
Eco130I CCWWGG 2 cut(s) 43, 617
Eco147I AGGCCT 1 cut(s) 634
Eco24I GRGCYC 1 cut(s) 570
Eco57I CTGAAG 1 cut(s) 914
EcoRII CCWGG 2 cut(s) 361, 1019
EcoT14I CCWWGG 2 cut(s) 43, 617
EcoT22I ATGCAT 1 cut(s) 496
EcoT38I GRGCYC 1 cut(s) 570
ErhI CCWWGG 2 cut(s) 43, 617
FaeI CATG 6 cut(s) 110, 217, 446, 498, 761, 950
FatI CATG 6 cut(s) 106, 213, 442, 494, 757, 946
FauI CCCGC 1 cut(s) 594
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 7 cut(s) 232, 247, 503, 517, 559, 600, 740
FriOI GRGCYC 1 cut(s) 570
Fsp4HI GCNGC 1 cut(s) 195
FspBI CTAG 2 cut(s) 182, 431
GlaI GCGC 1 cut(s) 531
GluI GCNGC 1 cut(s) 195
HaeII RGCGCY 1 cut(s) 533
HaeIII GGCC 2 cut(s) 48, 634
HapII CCGG 2 cut(s) 329, 919
HhaI GCGC 1 cut(s) 532
Hin1II CATG 6 cut(s) 110, 217, 446, 498, 761, 950
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HinfI GANTC 4 cut(s) 239, 305, 427, 730
HpaII CCGG 2 cut(s) 329, 919
HphI GGTGA 7 cut(s) 19, 43, 303, 893, 946, 947, 953
Hpy166II GTNNAC 4 cut(s) 403, 420, 716, 937
Hpy188I TCNGA 2 cut(s) 304, 630
Hpy188III TCNNGA 5 cut(s) 107, 165, 449, 473, 707
Hpy8I GTNNAC 4 cut(s) 403, 420, 716, 937
HpyAV CCTTC 5 cut(s) 291, 704, 776, 889, 902
HpyCH4III ACNGT 1 cut(s) 720
HpyCH4V TGCA 2 cut(s) 420, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
HpyF3I CTNAG 4 cut(s) 450, 629, 636, 687
Hsp92II CATG 6 cut(s) 110, 217, 446, 498, 761, 950
HspAI GCGC 1 cut(s) 530
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 5 cut(s) 24, 161, 208, 358, 469
LmnI GCTCC 4 cut(s) 120, 565, 658, 993
LweI GCATC 2 cut(s) 91, 481
MabI ACCWGGT 1 cut(s) 1019
MaeI CTAG 2 cut(s) 182, 431
MaeIII GTNAC 3 cut(s) 177, 309, 1012
MalI GATC 5 cut(s) 26, 163, 210, 360, 471
MboI GATC 5 cut(s) 24, 161, 208, 358, 469
MboII GAAGA 9 cut(s) 234, 779, 803, 815, 818, 821, 824, 827, 833
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 2 cut(s) 422, 570
MluCI AATT 4 cut(s) 341, 435, 824, 849
MlyI GAGTC 1 cut(s) 436
MmeI TCCRAC 1 cut(s) 636
Mph1103I ATGCAT 1 cut(s) 496
MseI TTAA 3 cut(s) 122, 149, 522
MslI CAYNNNNRTG 2 cut(s) 124, 756
MspI CCGG 2 cut(s) 329, 919
MspR9I CCNGG 2 cut(s) 363, 1021
MvaI CCWGG 2 cut(s) 363, 1021
MwoI GCNNNNNNNGC 1 cut(s) 219
NdeII GATC 5 cut(s) 24, 161, 208, 358, 469
NlaIII CATG 6 cut(s) 110, 217, 446, 498, 761, 950
NlaIV GGNNCC 2 cut(s) 567, 995
NmuCI GTSAC 2 cut(s) 309, 1012
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 1 cut(s) 498
PagI TCATGA 1 cut(s) 106
PceI AGGCCT 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 3 cut(s) 239, 305, 730
PkrI GCNGC 1 cut(s) 196
Ple19I CGATCG 1 cut(s) 211
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
Psp6I CCWGG 2 cut(s) 361, 1019
PspGI CCWGG 2 cut(s) 361, 1019
PspN4I GGNNCC 2 cut(s) 567, 995
PsuI RGATCY 1 cut(s) 358
PvuI CGATCG 1 cut(s) 211
RsaI GTAC 1 cut(s) 798
RsaNI GTAC 1 cut(s) 797
RseI CAYNNNNRTG 2 cut(s) 124, 756
SaqAI TTAA 3 cut(s) 122, 149, 522
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 5 cut(s) 24, 161, 208, 358, 469
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 2 cut(s) 363, 1021
SduI GDGCHC 2 cut(s) 422, 570
SexAI ACCWGGT 1 cut(s) 1019
SfaNI GCATC 2 cut(s) 91, 481
SfcI CTRYAG 1 cut(s) 156
SgrAI CRCCGGYG 2 cut(s) 328, 918
SmiMI CAYNNNNRTG 2 cut(s) 124, 756
Sse9I AATT 4 cut(s) 341, 435, 824, 849
SseBI AGGCCT 1 cut(s) 634
SsiI CCGC 5 cut(s) 195, 273, 314, 587, 940
SspI AATATT 1 cut(s) 777
SspMI CTAG 2 cut(s) 182, 431
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 2 cut(s) 361, 1019
StyI CCWWGG 2 cut(s) 43, 617
TaaI ACNGT 1 cut(s) 720
TaqI TCGA 2 cut(s) 483, 518
TasI AATT 4 cut(s) 341, 435, 824, 849
TatI WGTACW 1 cut(s) 796
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 3 cut(s) 239, 305, 730
Tru1I TTAA 3 cut(s) 122, 149, 522
Tru9I TTAA 3 cut(s) 122, 149, 522
TseFI GTSAC 2 cut(s) 309, 1012
Tsp45I GTSAC 2 cut(s) 309, 1012
TspDTI ATGAA 6 cut(s) 123, 230, 234, 367, 582, 774
TspGWI ACGGA 3 cut(s) 85, 437, 680
VneI GTGCAC 1 cut(s) 418
XapI RAATTY 2 cut(s) 341, 824
XceI RCATGY 1 cut(s) 498
XspI CTAG 2 cut(s) 182, 431
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.