RLG00000025493

Protein FANTASTIC FOUR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014217
Physical Location & Seq
Reverse (-)
46071198 .. 46072789
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025493

Sequence Viewer

Length: 1185 bp
ATGCACAAGGGCCAAAGCCCCCAGTTGGCACTGCGACTCATGCCAGGCCTCAGACCACTCTCCTCCCTCTCTATTAATACCTCCTCTTCCTCCACTCCCTCCTCTCCATTTTTCATCCCCGCAAAAACGTATCCATCATTTTTCATGACCCTTTTTGTTCTAATCGTTCCACTACTGACTTTTCTCTTCCTCTCTTCTGCGGCTTATTACAAGCTTCTTAATCTCCCAATCATTATGATGAGCTTCTGTAGAAAAATCACCCCTCACTCTCTTCTCGGCCTCACCGCCACCACCACAACCACCGCAACTCCTTCCACTACCAATGAAAACCTCCACCACTACATTCCTTCTCTGGCGGGAGGACTATTAGGGTTAGTTACTGCAACCTCCAATGACGTCACGCAGCGTCATCCTCTTGTTTTGGAATCTTCCTCCGTAAAGAAACAGAGCTCGTTGTCATTGCTCCCGTCGTTTTTACCGCCTCCTGCAACCAAGGCTGTAGGAGGCGGTGACATCGGCGGGTTCGGCTTCCTAGACGAAGTCGGTGGCGGCGTGGACGGGTTTGTGTCATGCACCGAGAGTCTAGGGTTTGAGAGCTCGGATGAGATCATGCGAGTGGACGATCAGGTTGAGCAGGATGACGAGAAGGATGAGCTCAGGTTGATGAGGCAGTCTGAGAACAAGAGGACCAAATGGAGGAGGATGAGAGATAAGAGGTCGTCGGTGGATAAGGTCAACAATAAGTTTCCGCCACCGATCTCTTCCTTGAATAAGAACGGACACCCGAATTTCTATCTCCGGCCGGTGAGGAAAGACGGGAGGTTGGAACTCACCGAGGTGAAGATTCATAGGCCAGAGGTTCTACGCGCTCATCGCGAAGATGGACGGTTGAGGTTGCATCTTATTACCCATGACCAAGAAGAAGACTTAATAGAAGAAGAGCAAGAAGAAGAAATTGAAGAGGAAGGAGTTGATGTTGAAGATATTAATAATGTCGAGGAAGGGAAAGAGGATGTAGAAATAGAAAGTAGTGTGAATGTTGGGGATCAGTGGAAGATGGGGGTGGCTGGAGGAGAAGCGTTTAGGCGGTGTCAGGATGTGGCGGGGAGTAGCCACCATGTGCACCACCACCACCACCACCACAACACCCACCCTGTGTGGAGCCAGCAGCATTGTGTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAF

Protein Analysis

395

Amino Acids

44.02

Weight (kDa)

5.89

Isoelectric Point (pI)

71.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF PF11250 248 - 301 8.9e-21 Fantastic Four meristem regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014217)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54740
fragaria_vesca FvH4_6g05230
malus_domestica MD04G1210600.v1.1 MD12G1224900.v1.1
prunus_persica Prupe.6G331500_v2.0.a1
pyrus_communis pycom04g18650 pycom12g21030
rosa_chinensis RchiOBHm_Chr3g0453941
rosa_laevigata RLG00000025493
rosa_multiflora Rmu_sc0003556.1_g000030
rosa_roxburghii Rroxscaffold_6G00430780
rosa_rugosa Rorug02G0651900
rosa_samantha Rh3AG054500 Rh3BG056100 Rh3CG055300 Rh3DG056600
rosa_wichuraiana Rw3G004180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 399
AccII CGCG 2 cut(s) 867, 876
AclWI GGATC 1 cut(s) 1053
AcoI YGGCCR 1 cut(s) 800
AcsI RAATTY 1 cut(s) 787
AcyI GRCGYC 1 cut(s) 396
AdeI CACNNNGTG 2 cut(s) 1120, 1156
AfiI CCNNNNNNNGG 2 cut(s) 25, 696
AgsI TTSAA 3 cut(s) 769, 959, 980
AjnI CCWGG 1 cut(s) 43
AleI CACNNNNGTG 1 cut(s) 836
AluBI AGCT 5 cut(s) 214, 243, 450, 597, 655
AluI AGCT 5 cut(s) 214, 243, 450, 597, 655
Alw21I GWGCWC 4 cut(s) 452, 599, 657, 1125
Alw44I GTGCAC 1 cut(s) 1121
AlwI GGATC 1 cut(s) 1053
AoxI GGCC 5 cut(s) 10, 46, 277, 800, 851
ApaLI GTGCAC 1 cut(s) 1121
ApeKI GCWGC 2 cut(s) 403, 1168
ApoI RAATTY 1 cut(s) 787
AseI ATTAAT 2 cut(s) 75, 987
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 2 cut(s) 10, 687
AsuHPI GGTGA 6 cut(s) 250, 274, 521, 817, 823, 850
AvaII GGWCC 1 cut(s) 687
BaeGI GKGCMC 1 cut(s) 1125
BanII GRGCYC 3 cut(s) 452, 599, 657
BbsI GAAGAC 1 cut(s) 930
Bbv12I GWGCWC 4 cut(s) 452, 599, 657, 1125
BbvI GCAGC 2 cut(s) 415, 1180
BccI CCATC 3 cut(s) 142, 875, 1051
BciT130I CCWGG 1 cut(s) 45
BciVI GTATCC 1 cut(s) 141
BfaI CTAG 2 cut(s) 533, 584
BfmI CTRYAG 2 cut(s) 247, 498
BfuI GTATCC 1 cut(s) 141
BisI GCNGC 4 cut(s) 201, 404, 550, 1169
BlsI GCNGC 4 cut(s) 202, 405, 551, 1170
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 687
BmgT120I GGNCC 2 cut(s) 10, 687
BmiI GGNNCC 1 cut(s) 1163
BmrFI CCNGG 1 cut(s) 45
BmrI ACTGGG 1 cut(s) 16
BmsI GCATC 1 cut(s) 907
BmuI ACTGGG 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 930
BpmI CTGGAG 1 cut(s) 1089
Bpu10I CCTNAGC 1 cut(s) 656
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 2 cut(s) 492, 834
BsaXI ACNNNNNCTCC 4 cut(s) 292, 322, 960, 990
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 696
Bse118I RCCGGY 1 cut(s) 802
Bse1I ACTGG 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 458
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 492, 834
BseGI GGATG 8 cut(s) 114, 409, 607, 643, 655, 708, 1018, 1102
BseLI CCNNNNNNNGG 2 cut(s) 25, 696
BseMI GCAATG 1 cut(s) 458
BseMII CTCAG 3 cut(s) 64, 666, 670
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 5 cut(s) 52, 73, 91, 712, 1086
BseSI GKGCMC 1 cut(s) 1125
BseX3I CGGCCG 1 cut(s) 800
BseXI GCAGC 2 cut(s) 415, 1180
Bsh1236I CGCG 2 cut(s) 867, 876
Bsh1285I CGRYCG 1 cut(s) 803
BshFI GGCC 5 cut(s) 12, 48, 279, 802, 853
BsiEI CGRYCG 1 cut(s) 803
BsiHKAI GWGCWC 4 cut(s) 452, 599, 657, 1125
BsiSI CCGG 2 cut(s) 799, 803
BslI CCNNNNNNNGG 2 cut(s) 25, 696
BsnI GGCC 5 cut(s) 12, 48, 279, 802, 853
Bsp1286I GDGCHC 4 cut(s) 452, 599, 657, 1125
Bsp143I GATC 4 cut(s) 606, 622, 756, 1045
Bsp68I TCGCGA 1 cut(s) 876
BspANI GGCC 5 cut(s) 12, 48, 279, 802, 853
BspCNI CTCAG 3 cut(s) 63, 667, 669
BspFNI CGCG 2 cut(s) 867, 876
BspHI TCATGA 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 1163
BspPI GGATC 1 cut(s) 1053
BspQI GCTCTTC 1 cut(s) 933
BsrDI GCAATG 1 cut(s) 458
BsrFI RCCGGY 1 cut(s) 802
BsrI ACTGG 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 802
BssECI CCNNGG 2 cut(s) 492, 834
BssMI GATC 4 cut(s) 606, 622, 756, 1045
BssNI GRCGYC 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 1 cut(s) 888
Bst6I CTCTTC 7 cut(s) 91, 191, 199, 276, 766, 933, 954
BstACI GRCGYC 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 1166
BstDEI CTNAG 3 cut(s) 50, 656, 675
BstF5I GGATG 8 cut(s) 114, 409, 607, 643, 655, 708, 1018, 1102
BstFNI CGCG 2 cut(s) 867, 876
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 4 cut(s) 609, 625, 759, 1048
BstMBI GATC 4 cut(s) 606, 622, 756, 1045
BstMCI CGRYCG 1 cut(s) 803
BstMWI GCNNNNNNNGC 4 cut(s) 40, 494, 525, 873
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 2 cut(s) 247, 498
BstSLI GKGCMC 1 cut(s) 1125
BstUI CGCG 2 cut(s) 867, 876
BstV1I GCAGC 2 cut(s) 415, 1180
BstV2I GAAGAC 1 cut(s) 930
BstZI CGGCCG 1 cut(s) 800
BsuI GTATCC 1 cut(s) 141
BsuRI GGCC 5 cut(s) 12, 48, 279, 802, 853
BtgZI GCGATG 1 cut(s) 857
BtsCI GGATG 8 cut(s) 114, 409, 607, 643, 655, 708, 1018, 1102
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 29, 1055
BtuMI TCGCGA 1 cut(s) 876
Cac8I GCNNGC 1 cut(s) 1166
CciI TCATGA 1 cut(s) 144
CfoI GCGC 1 cut(s) 869
Cfr10I RCCGGY 1 cut(s) 802
Cfr13I GGNCC 2 cut(s) 10, 687
CseI GACGC 1 cut(s) 395
CviAII CATG 6 cut(s) 40, 145, 570, 610, 911, 1118
DdeI CTNAG 3 cut(s) 50, 656, 675
DpnI GATC 4 cut(s) 608, 624, 758, 1047
DpnII GATC 4 cut(s) 606, 622, 756, 1045
DraIII CACNNNGTG 2 cut(s) 1120, 1156
EaeI YGGCCR 1 cut(s) 800
EagI CGGCCG 1 cut(s) 800
Eam1104I CTCTTC 7 cut(s) 91, 191, 199, 276, 766, 933, 954
EarI CTCTTC 7 cut(s) 91, 191, 199, 276, 766, 933, 954
EciI GGCGGA 1 cut(s) 738
Ecl136II GAGCTC 3 cut(s) 450, 597, 655
EclXI CGGCCG 1 cut(s) 800
Eco130I CCWWGG 1 cut(s) 492
Eco147I AGGCCT 1 cut(s) 48
Eco24I GRGCYC 3 cut(s) 452, 599, 657
Eco47I GGWCC 1 cut(s) 687
Eco52I CGGCCG 1 cut(s) 800
Eco53kI GAGCTC 3 cut(s) 450, 597, 655
EcoICRI GAGCTC 3 cut(s) 450, 597, 655
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 492
EcoT38I GRGCYC 3 cut(s) 452, 599, 657
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 6 cut(s) 43, 148, 573, 613, 914, 1121
FaiI YATR 8 cut(s) 41, 146, 236, 571, 611, 849, 912, 1119
FatI CATG 6 cut(s) 39, 144, 569, 609, 910, 1117
FauI CCCGC 4 cut(s) 127, 349, 512, 1096
Fnu4HI GCNGC 4 cut(s) 201, 404, 550, 1169
FokI GGATG 8 cut(s) 101, 396, 614, 650, 662, 715, 1025, 1109
FriOI GRGCYC 3 cut(s) 452, 599, 657
Fsp4HI GCNGC 4 cut(s) 201, 404, 550, 1169
FspBI CTAG 2 cut(s) 533, 584
GlaI GCGC 1 cut(s) 868
GluI GCNGC 4 cut(s) 201, 404, 550, 1169
GsuI CTGGAG 1 cut(s) 1089
HaeIII GGCC 5 cut(s) 12, 48, 279, 802, 853
HapII CCGG 2 cut(s) 799, 803
HgaI GACGC 1 cut(s) 395
HhaI GCGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 6 cut(s) 43, 148, 573, 613, 914, 1121
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 4 cut(s) 36, 425, 580, 844
HpaII CCGG 2 cut(s) 799, 803
HphI GGTGA 6 cut(s) 250, 274, 521, 817, 823, 850
Hpy166II GTNNAC 4 cut(s) 556, 619, 736, 1123
Hpy188I TCNGA 3 cut(s) 53, 601, 676
Hpy188III TCNNGA 3 cut(s) 145, 875, 1094
Hpy8I GTNNAC 4 cut(s) 556, 619, 736, 1123
Hpy99I CGWCG 2 cut(s) 472, 724
HpyAV CCTTC 5 cut(s) 321, 357, 640, 959, 995
HpyCH4III ACNGT 1 cut(s) 888
HpyCH4IV ACGT 2 cut(s) 128, 396
HpyCH4V TGCA 6 cut(s) 4, 383, 488, 573, 898, 1123
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 494, 525, 873
HpyF3I CTNAG 3 cut(s) 50, 656, 675
HpySE526I ACGT 2 cut(s) 128, 396
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 6 cut(s) 43, 148, 573, 613, 914, 1121
HspAI GCGC 1 cut(s) 867
Kzo9I GATC 4 cut(s) 606, 622, 756, 1045
LguI GCTCTTC 1 cut(s) 933
LmnI GCTCC 2 cut(s) 468, 1161
Lsp1109I GCAGC 2 cut(s) 415, 1180
LweI GCATC 1 cut(s) 907
MaeI CTAG 2 cut(s) 533, 584
MaeII ACGT 2 cut(s) 128, 396
MaeIII GTNAC 3 cut(s) 376, 397, 509
MalI GATC 4 cut(s) 608, 624, 758, 1047
MboI GATC 4 cut(s) 606, 622, 756, 1045
MhlI GDGCHC 4 cut(s) 452, 599, 657, 1125
MluCI AATT 2 cut(s) 787, 954
MlyI GAGTC 2 cut(s) 30, 589
MmeI TCCRAC 1 cut(s) 804
MseI TTAA 4 cut(s) 75, 219, 929, 987
MslI CAYNNNNRTG 3 cut(s) 236, 614, 836
MspI CCGG 2 cut(s) 799, 803
MspR9I CCNGG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 45
MvnI CGCG 2 cut(s) 867, 876
MwoI GCNNNNNNNGC 4 cut(s) 40, 494, 525, 873
NdeII GATC 4 cut(s) 606, 622, 756, 1045
NlaIII CATG 6 cut(s) 43, 148, 573, 613, 914, 1121
NlaIV GGNNCC 1 cut(s) 1163
NmeAIII GCCGAG 1 cut(s) 255
NmuCI GTSAC 2 cut(s) 397, 509
NruI TCGCGA 1 cut(s) 876
OliI CACNNNNGTG 1 cut(s) 836
PagI TCATGA 1 cut(s) 144
PceI AGGCCT 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 933
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 2 cut(s) 425, 844
PflFI GACNNNGTC 1 cut(s) 539
PkrI GCNGC 4 cut(s) 202, 405, 551, 1170
PleI GAGTC 2 cut(s) 30, 588
PpsI GAGTC 2 cut(s) 30, 588
PshBI ATTAAT 2 cut(s) 75, 987
Psp124BI GAGCTC 3 cut(s) 452, 599, 657
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 1163
PspPI GGNCC 2 cut(s) 10, 687
PsyI GACNNNGTC 1 cut(s) 539
RruI TCGCGA 1 cut(s) 876
RseI CAYNNNNRTG 3 cut(s) 236, 614, 836
SacI GAGCTC 3 cut(s) 452, 599, 657
SapI GCTCTTC 1 cut(s) 933
SaqAI TTAA 4 cut(s) 75, 219, 929, 987
SatI GCNGC 4 cut(s) 201, 404, 550, 1169
Sau3AI GATC 4 cut(s) 606, 622, 756, 1045
Sau96I GGNCC 2 cut(s) 10, 687
SchI GAGTC 2 cut(s) 30, 589
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 4 cut(s) 452, 599, 657, 1125
SfaNI GCATC 1 cut(s) 907
SfcI CTRYAG 2 cut(s) 247, 498
SinI GGWCC 1 cut(s) 687
SmiMI CAYNNNNRTG 3 cut(s) 236, 614, 836
Sse9I AATT 2 cut(s) 787, 954
SseBI AGGCCT 1 cut(s) 48
SspMI CTAG 2 cut(s) 533, 584
SstI GAGCTC 3 cut(s) 452, 599, 657
StuI AGGCCT 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 1 cut(s) 888
TaiI ACGT 2 cut(s) 131, 399
TaqI TCGA 1 cut(s) 996
TasI AATT 2 cut(s) 787, 954
TauI GCSGC 2 cut(s) 203, 552
TfiI GAWTC 2 cut(s) 425, 844
Tru1I TTAA 4 cut(s) 75, 219, 929, 987
Tru9I TTAA 4 cut(s) 75, 219, 929, 987
TscAI CASTG 2 cut(s) 36, 1055
TseFI GTSAC 2 cut(s) 397, 509
TseI GCWGC 2 cut(s) 403, 1168
Tsp45I GTSAC 2 cut(s) 397, 509
TspDTI ATGAA 4 cut(s) 103, 133, 339, 836
TspGWI ACGGA 2 cut(s) 424, 792
TspRI CASTG 2 cut(s) 36, 1055
Tth111I GACNNNGTC 1 cut(s) 539
VneI GTGCAC 1 cut(s) 1121
VpaK11BI GGWCC 1 cut(s) 687
VspI ATTAAT 2 cut(s) 75, 987
XapI RAATTY 1 cut(s) 787
XspI CTAG 2 cut(s) 533, 584
ZraI GACGTC 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.