Rorug02G0651900

Protein FANTASTIC FOUR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014217
Physical Location & Seq
Reverse (-)
75951458 .. 75954864
3407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0651900.1

Sequence Viewer

Length: 1449 bp
ATGAGAGGTCAGAAGGGAGGGTTGAATAGCAGCAGCAGGCGACCCAGCACCGTTCTGTACCTGGTTTTCGCTGCCGCATTCTTCTCTCTCCTCGTCTTCGCCATCCAGACCTCTTTCTTCGCTCAATCTGATTCCTCTTCCGCTTCAGTTAAGTTAGATCTCAATACTCAGCAAGATATTCGCACTTTATCCCAATTTCAGTCCACTGTGCAGCAATGCGTGGCAAACAGAGGGCTTGGTCTTACTGCAGATGTTGTTGACCACTGCAATCTGATTCTCAAGTATCCACAAGGAACTAACAGCACCTGGTACAATGCGCAGTTTAAAGTTTTTGAACCATTACAGTACAAGTTCAATGTTTGTGAGGCACTCTTATTGTGGGAGCAGTACCGTAACATGACTACAGTATTGACAAGGGAGTATCTAGATGTTCGCCCTAAAGGATGGGAAGACTACGCACCACAAAGGATAGCGCAATTGGGAACCAAAAATTGCTACAATAAGACAATTTGTGAGGAACACCTTAATTTGATACTGCCGGCAAAGCCACCTTTCCATCCTCGCCAATTCCGTACATGTGCAGTTGTTGGAAATTCAGGAGATCTTTTGAAAACAGAGTTTGGAAAAGAAATTGATGCCCATGATGCTGTAATAAGAGACAATGAGGCTCCTGTTAATGAGAAATATGCCAAGTATGTTGGTCTCAAGAGGGATTTTCGCCTTGTTGTGAGGGGTGCTGCTCGTAACATGGTTGCCATTCTAAATGGGTCTGATGATGAGGTACTCATAATCAAGAGTGTTACACATAAAGACTTCAATGCAATGATCAAGAGTATTCCAAATCCAGTGTATCTTTTTCAAGGTATTGTGCTCCGCAGAGGTGCCAAAGGAACTGGAATGAAATCCATAGAACTAGCACTATCTATGTGTGACATTGTTGACATGTATGGTTTCACTGTTGATCCTGGCTATACTGAATGGACACGTTACTTCTCTACACCTAGGAAAGGGCACAATCCACTTCAAGGAAGGGCATACTACCAGCTCCTAGAGTGCCTTGGTGTCATCAGAATTCATTCTCCAATGAGATCTAAGAGGAAGCAAGGCTGGTCAGATATACCTAGTCGAGAAATGATAGGCAGAGCTCATGCAGCAGCCTTGCGTTTAAAGAGGGGTCAAGATGGTGATTTGGGACAGTTTGGTAGTTGTAAGGTGTCGGGTAATGTGGACCCTGACAAAATTGGGCCTGTCTCAGGATCTGCAGACATGAGTGATGTAAGAAAGTCTTCAAATTACAGTAAGTGGGAAGTCTTACCCTTTGAGAGTCTGAGGAAGGAAGCGCAGGACCACTATAATCAAATGGAAGGTGTGTCTCTATACAAAATGGATGGCAACAAGTTAGATGATCTAGTATGTGTGAGGCATTCCTCAAAATCAGCTGTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAF

Protein Analysis

482

Amino Acids

54.27

Weight (kDa)

9.14

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_29 PF00777 169 - 276 4.6e-19 Glycosyltransferase family 29 (sialyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014217)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54740
fragaria_vesca FvH4_6g05230
malus_domestica MD04G1210600.v1.1 MD12G1224900.v1.1
prunus_persica Prupe.6G331500_v2.0.a1
pyrus_communis pycom04g18650 pycom12g21030
rosa_chinensis RchiOBHm_Chr3g0453941
rosa_laevigata RLG00000025493
rosa_multiflora Rmu_sc0003556.1_g000030
rosa_roxburghii Rroxscaffold_6G00430780
rosa_rugosa Rorug02G0651900
rosa_samantha Rh3AG054500 Rh3BG056100 Rh3CG055300 Rh3DG056600
rosa_wichuraiana Rw3G004180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 881
AccI GTMKAC 1 cut(s) 1443
AciI CCGC 3 cut(s) 75, 141, 874
AclWI GGATC 2 cut(s) 956, 1264
AcsI RAATTY 2 cut(s) 592, 1071
AcuI CTGAAG 1 cut(s) 129
AfaI GTAC 6 cut(s) 59, 311, 347, 389, 574, 783
AfiI CCNNNNNNNGG 3 cut(s) 1007, 1025, 1253
AflIII ACRYGT 3 cut(s) 575, 942, 983
AgsI TTSAA 8 cut(s) 25, 335, 355, 610, 817, 860, 1025, 1290
AjnI CCWGG 3 cut(s) 60, 305, 964
AluBI AGCT 3 cut(s) 1045, 1145, 1439
AluI AGCT 3 cut(s) 1045, 1145, 1439
Alw21I GWGCWC 2 cut(s) 873, 1147
Alw26I GTCTC 4 cut(s) 651, 707, 1255, 1377
AlwI GGATC 2 cut(s) 956, 1264
AlwNI CAGNNNCTG 2 cut(s) 306, 1259
AoxI GGCC 1 cut(s) 1244
ApeKI GCWGC 7 cut(s) 30, 33, 71, 211, 737, 1151, 1154
ApoI RAATTY 2 cut(s) 592, 1071
Asp700I GAANNNNTTC 2 cut(s) 1075, 1285
AspA2I CCTAGG 1 cut(s) 1001
AspLEI GCGC 3 cut(s) 319, 475, 1342
AspS9I GGNCC 3 cut(s) 1228, 1244, 1345
AsuHPI GGTGA 1 cut(s) 1196
AvaII GGWCC 2 cut(s) 1228, 1345
AvrII CCTAGG 1 cut(s) 1001
BaeGI GKGCMC 1 cut(s) 1014
BanI GGYRCC 1 cut(s) 881
BanII GRGCYC 1 cut(s) 1147
BbsI GAAGAC 3 cut(s) 88, 456, 1278
Bbv12I GWGCWC 2 cut(s) 873, 1147
BbvI GCAGC 7 cut(s) 42, 45, 58, 223, 724, 1163, 1166
BccI CCATC 5 cut(s) 110, 438, 564, 1175, 1382
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BciT130I CCWGG 3 cut(s) 62, 307, 966
BciVI GTATCC 1 cut(s) 294
BclI TGATCA 1 cut(s) 825
BcoDI GTCTC 4 cut(s) 651, 707, 1255, 1377
BfaI CTAG 6 cut(s) 425, 914, 1002, 1049, 1122, 1409
BfmI CTRYAG 3 cut(s) 246, 402, 1260
BfuI GTATCC 1 cut(s) 294
BglII AGATCT 3 cut(s) 157, 601, 1088
BisI GCNGC 8 cut(s) 31, 34, 72, 75, 212, 738, 1152, 1155
BlnI CCTAGG 1 cut(s) 1001
BlsI GCNGC 8 cut(s) 32, 35, 73, 76, 213, 739, 1153, 1156
Bme1390I CCNGG 3 cut(s) 62, 307, 966
Bme18I GGWCC 2 cut(s) 1228, 1345
BmgT120I GGNCC 3 cut(s) 1228, 1244, 1345
BmiI GGNNCC 4 cut(s) 484, 669, 883, 1230
BmrFI CCNGG 3 cut(s) 62, 307, 966
BmsI GCATC 2 cut(s) 625, 634
BpiI GAAGAC 3 cut(s) 88, 456, 1278
BpuEI CTTGAG 2 cut(s) 263, 689
BsaI GGTCTC 1 cut(s) 707
BsaJI CCNNGG 2 cut(s) 1001, 1057
Bsc4I CCNNNNNNNGG 3 cut(s) 1007, 1025, 1253
Bse118I RCCGGY 1 cut(s) 538
Bse1I ACTGG 2 cut(s) 845, 898
Bse3DI GCAATG 2 cut(s) 221, 828
BseBI CCWGG 3 cut(s) 62, 307, 966
BseDI CCNNGG 2 cut(s) 1001, 1057
BseGI GGATG 4 cut(s) 102, 449, 556, 1393
BseLI CCNNNNNNNGG 3 cut(s) 1007, 1025, 1253
BseMI GCAATG 2 cut(s) 221, 828
BseMII CTCAG 3 cut(s) 182, 1266, 1319
BseNI ACTGG 2 cut(s) 845, 898
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 1014
BseXI GCAGC 7 cut(s) 42, 45, 58, 223, 724, 1163, 1166
BseYI CCCAGC 1 cut(s) 44
BsgI GTGCAG 2 cut(s) 230, 600
BshFI GGCC 1 cut(s) 1246
BshNI GGYRCC 1 cut(s) 881
BsiHKAI GWGCWC 2 cut(s) 873, 1147
BsiSI CCGG 1 cut(s) 539
BslFI GGGAC 1 cut(s) 1206
BslI CCNNNNNNNGG 3 cut(s) 1007, 1025, 1253
BsmAI GTCTC 4 cut(s) 651, 707, 1255, 1377
BsmFI GGGAC 1 cut(s) 1206
BsmI GAATGC 2 cut(s) 77, 1423
BsnI GGCC 1 cut(s) 1246
Bso31I GGTCTC 1 cut(s) 707
Bsp1286I GDGCHC 3 cut(s) 873, 1014, 1147
Bsp143I GATC 7 cut(s) 157, 601, 825, 961, 1088, 1256, 1405
BspACI CCGC 3 cut(s) 75, 141, 874
BspANI GGCC 1 cut(s) 1246
BspCNI CTCAG 3 cut(s) 181, 1265, 1320
BspLI GGNNCC 4 cut(s) 484, 669, 883, 1230
BspMAI CTGCAG 2 cut(s) 250, 1264
BspPI GGATC 2 cut(s) 956, 1264
BspT107I GGYRCC 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 707
BsrDI GCAATG 2 cut(s) 221, 828
BsrFI RCCGGY 1 cut(s) 538
BsrI ACTGG 2 cut(s) 845, 898
BssAI RCCGGY 1 cut(s) 538
BssECI CCNNGG 2 cut(s) 1001, 1057
BssMI GATC 7 cut(s) 157, 601, 825, 961, 1088, 1256, 1405
BssT1I CCWWGG 2 cut(s) 1001, 1057
Bst2UI CCWGG 3 cut(s) 62, 307, 966
Bst4CI ACNGT 8 cut(s) 52, 208, 345, 392, 406, 958, 1197, 1298
Bst6I CTCTTC 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 38, 540
BstDEI CTNAG 4 cut(s) 168, 1092, 1252, 1328
BstENI CCTNNNNNAGG 2 cut(s) 1005, 1251
BstF5I GGATG 4 cut(s) 102, 449, 556, 1393
BstHHI GCGC 3 cut(s) 319, 475, 1342
BstKTI GATC 7 cut(s) 160, 604, 828, 964, 1091, 1259, 1408
BstMAI GTCTC 4 cut(s) 651, 707, 1255, 1377
BstMBI GATC 7 cut(s) 157, 601, 825, 961, 1088, 1256, 1405
BstMWI GCNNNNNNNGC 3 cut(s) 544, 644, 1151
BstNI CCWGG 3 cut(s) 62, 307, 966
BstNSI RCATGY 2 cut(s) 579, 946
BstSCI CCNGG 3 cut(s) 60, 305, 964
BstSFI CTRYAG 3 cut(s) 246, 402, 1260
BstSLI GKGCMC 1 cut(s) 1014
BstV1I GCAGC 7 cut(s) 42, 45, 58, 223, 724, 1163, 1166
BstV2I GAAGAC 3 cut(s) 88, 456, 1278
BstX2I RGATCY 4 cut(s) 157, 601, 1088, 1256
BstYI RGATCY 4 cut(s) 157, 601, 1088, 1256
BsuI GTATCC 1 cut(s) 294
BsuRI GGCC 1 cut(s) 1246
BtsCI GGATG 4 cut(s) 102, 449, 556, 1393
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 4 cut(s) 204, 262, 852, 954
Cac8I GCNNGC 2 cut(s) 38, 540
CaiI CAGNNNCTG 2 cut(s) 306, 1259
CfoI GCGC 3 cut(s) 319, 475, 1342
Cfr10I RCCGGY 1 cut(s) 538
Cfr13I GGNCC 3 cut(s) 1228, 1244, 1345
CsiI ACCWGGT 2 cut(s) 60, 305
Csp6I GTAC 6 cut(s) 58, 310, 346, 388, 573, 782
CviAII CATG 7 cut(s) 397, 576, 641, 748, 943, 1148, 1267
CviQI GTAC 6 cut(s) 58, 310, 346, 388, 573, 782
DdeI CTNAG 4 cut(s) 168, 1092, 1252, 1328
DpnI GATC 7 cut(s) 159, 603, 827, 963, 1090, 1258, 1407
DpnII GATC 7 cut(s) 157, 601, 825, 961, 1088, 1256, 1405
DraI TTTAAA 2 cut(s) 325, 1167
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 1145
Eco130I CCWWGG 2 cut(s) 1001, 1057
Eco24I GRGCYC 1 cut(s) 1147
Eco31I GGTCTC 1 cut(s) 707
Eco47I GGWCC 2 cut(s) 1228, 1345
Eco53kI GAGCTC 1 cut(s) 1145
Eco57I CTGAAG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 1145
EcoNI CCTNNNNNAGG 2 cut(s) 1005, 1251
EcoRI GAATTC 1 cut(s) 1071
EcoRII CCWGG 3 cut(s) 60, 305, 964
EcoT14I CCWWGG 2 cut(s) 1001, 1057
EcoT38I GRGCYC 1 cut(s) 1147
ErhI CCWWGG 2 cut(s) 1001, 1057
FaeI CATG 7 cut(s) 400, 579, 644, 751, 946, 1151, 1270
FalI AAGNNNNNCTT 2 cut(s) 1270, 1302
FaqI GGGAC 1 cut(s) 1206
FatI CATG 7 cut(s) 396, 575, 640, 747, 942, 1147, 1266
FbaI TGATCA 1 cut(s) 825
FblI GTMKAC 1 cut(s) 1443
Fnu4HI GCNGC 8 cut(s) 31, 34, 72, 75, 212, 738, 1152, 1155
FokI GGATG 4 cut(s) 89, 456, 543, 1400
FriOI GRGCYC 1 cut(s) 1147
Fsp4HI GCNGC 8 cut(s) 31, 34, 72, 75, 212, 738, 1152, 1155
FspBI CTAG 6 cut(s) 425, 914, 1002, 1049, 1122, 1409
FspI TGCGCA 1 cut(s) 318
GlaI GCGC 3 cut(s) 318, 474, 1341
GluI GCNGC 8 cut(s) 31, 34, 72, 75, 212, 738, 1152, 1155
GsaI CCCAGC 1 cut(s) 48
HaeIII GGCC 1 cut(s) 1246
HapII CCGG 1 cut(s) 539
HhaI GCGC 3 cut(s) 319, 475, 1342
Hin1II CATG 7 cut(s) 400, 579, 644, 751, 946, 1151, 1270
Hin6I GCGC 3 cut(s) 317, 473, 1340
HinP1I GCGC 3 cut(s) 317, 473, 1340
HincII GTYRAC 2 cut(s) 259, 940
HindII GTYRAC 2 cut(s) 259, 940
HinfI GANTC 3 cut(s) 131, 274, 1324
HpaII CCGG 1 cut(s) 539
HphI GGTGA 1 cut(s) 1196
Hpy166II GTNNAC 5 cut(s) 204, 259, 940, 1228, 1444
Hpy188I TCNGA 7 cut(s) 12, 130, 273, 772, 1070, 1114, 1329
Hpy188III TCNNGA 9 cut(s) 106, 425, 597, 706, 793, 829, 1127, 1178, 1254
Hpy8I GTNNAC 5 cut(s) 204, 259, 940, 1228, 1444
HpyAV CCTTC 4 cut(s) 7, 1023, 1327, 1358
HpyCH4III ACNGT 8 cut(s) 52, 208, 345, 392, 406, 958, 1197, 1298
HpyCH4IV ACGT 1 cut(s) 985
HpyCH4V TGCA 7 cut(s) 211, 248, 267, 581, 821, 1151, 1262
HpyF10VI GCNNNNNNNGC 3 cut(s) 544, 644, 1151
HpyF3I CTNAG 4 cut(s) 168, 1092, 1252, 1328
HpySE526I ACGT 1 cut(s) 985
Hsp92II CATG 7 cut(s) 400, 579, 644, 751, 946, 1151, 1270
HspAI GCGC 3 cut(s) 317, 473, 1340
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 7 cut(s) 157, 601, 825, 961, 1088, 1256, 1405
LmnI GCTCC 4 cut(s) 382, 673, 876, 1050
Lsp1109I GCAGC 7 cut(s) 42, 45, 58, 223, 724, 1163, 1166
LweI GCATC 2 cut(s) 625, 634
MabI ACCWGGT 2 cut(s) 60, 305
MaeI CTAG 6 cut(s) 425, 914, 1002, 1049, 1122, 1409
MaeII ACGT 1 cut(s) 985
MaeIII GTNAC 5 cut(s) 392, 743, 799, 929, 986
MalI GATC 7 cut(s) 159, 603, 827, 963, 1090, 1258, 1407
MboI GATC 7 cut(s) 157, 601, 825, 961, 1088, 1256, 1405
MboII GAAGA 6 cut(s) 73, 88, 109, 129, 461, 1278
MfeI CAATTG 1 cut(s) 476
MflI RGATCY 4 cut(s) 157, 601, 1088, 1256
MhlI GDGCHC 3 cut(s) 873, 1014, 1147
MlyI GAGTC 1 cut(s) 1333
MmeI TCCRAC 1 cut(s) 568
MroNI GCCGGC 1 cut(s) 538
MroXI GAANNNNTTC 2 cut(s) 1075, 1285
MseI TTAA 5 cut(s) 150, 324, 525, 675, 1166
MspA1I CMGCKG 1 cut(s) 1439
MspI CCGG 1 cut(s) 539
MspR9I CCNGG 3 cut(s) 62, 307, 966
MunI CAATTG 1 cut(s) 476
Mva1269I GAATGC 2 cut(s) 77, 1423
MvaI CCWGG 3 cut(s) 62, 307, 966
MwoI GCNNNNNNNGC 3 cut(s) 544, 644, 1151
NaeI GCCGGC 1 cut(s) 540
NdeII GATC 7 cut(s) 157, 601, 825, 961, 1088, 1256, 1405
NgoMIV GCCGGC 1 cut(s) 538
NlaIII CATG 7 cut(s) 400, 579, 644, 751, 946, 1151, 1270
NlaIV GGNNCC 4 cut(s) 484, 669, 883, 1230
NmuCI GTSAC 1 cut(s) 929
NsbI TGCGCA 1 cut(s) 318
NspI RCATGY 2 cut(s) 579, 946
PciI ACATGT 2 cut(s) 575, 942
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PctI GAATGC 2 cut(s) 77, 1423
PdiI GCCGGC 1 cut(s) 540
PdmI GAANNNNTTC 2 cut(s) 1075, 1285
PfeI GAWTC 2 cut(s) 131, 274
PkrI GCNGC 8 cut(s) 32, 35, 73, 76, 213, 739, 1153, 1156
PleI GAGTC 1 cut(s) 1332
PpsI GAGTC 1 cut(s) 1332
PscI ACATGT 2 cut(s) 575, 942
Psp124BI GAGCTC 1 cut(s) 1147
Psp6I CCWGG 3 cut(s) 60, 305, 964
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 3 cut(s) 60, 305, 964
PspN4I GGNNCC 4 cut(s) 484, 669, 883, 1230
PspPI GGNCC 3 cut(s) 1228, 1244, 1345
PstI CTGCAG 2 cut(s) 250, 1264
PstNI CAGNNNCTG 2 cut(s) 306, 1259
PsuI RGATCY 4 cut(s) 157, 601, 1088, 1256
PvuII CAGCTG 1 cut(s) 1439
RsaI GTAC 6 cut(s) 59, 311, 347, 389, 574, 783
RsaNI GTAC 6 cut(s) 58, 310, 346, 388, 573, 782
SacI GAGCTC 1 cut(s) 1147
SaqAI TTAA 5 cut(s) 150, 324, 525, 675, 1166
SatI GCNGC 8 cut(s) 31, 34, 72, 75, 212, 738, 1152, 1155
Sau3AI GATC 7 cut(s) 157, 601, 825, 961, 1088, 1256, 1405
Sau96I GGNCC 3 cut(s) 1228, 1244, 1345
SchI GAGTC 1 cut(s) 1333
ScrFI CCNGG 3 cut(s) 62, 307, 966
SduI GDGCHC 3 cut(s) 873, 1014, 1147
SexAI ACCWGGT 2 cut(s) 60, 305
SfaNI GCATC 2 cut(s) 625, 634
SfcI CTRYAG 3 cut(s) 246, 402, 1260
SinI GGWCC 2 cut(s) 1228, 1345
SmlI CTYRAG 2 cut(s) 278, 704
SmoI CTYRAG 2 cut(s) 278, 704
SsiI CCGC 3 cut(s) 75, 141, 874
SspMI CTAG 6 cut(s) 425, 914, 1002, 1049, 1122, 1409
SstI GAGCTC 1 cut(s) 1147
StyD4I CCNGG 3 cut(s) 60, 305, 964
StyI CCWWGG 2 cut(s) 1001, 1057
TaaI ACNGT 8 cut(s) 52, 208, 345, 392, 406, 958, 1197, 1298
TaiI ACGT 1 cut(s) 988
TaqI TCGA 1 cut(s) 1126
TatI WGTACW 1 cut(s) 345
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 2 cut(s) 131, 274
Tru1I TTAA 5 cut(s) 150, 324, 525, 675, 1166
Tru9I TTAA 5 cut(s) 150, 324, 525, 675, 1166
TscAI CASTG 4 cut(s) 211, 269, 852, 961
TseFI GTSAC 1 cut(s) 929
TseI GCWGC 7 cut(s) 30, 33, 71, 211, 737, 1151, 1154
Tsp45I GTSAC 1 cut(s) 929
TspDTI ATGAA 2 cut(s) 914, 1064
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 4 cut(s) 211, 269, 852, 961
VpaK11BI GGWCC 2 cut(s) 1228, 1345
XagI CCTNNNNNAGG 2 cut(s) 1005, 1251
XapI RAATTY 2 cut(s) 592, 1071
XbaI TCTAGA 1 cut(s) 424
XceI RCATGY 2 cut(s) 579, 946
XmaJI CCTAGG 1 cut(s) 1001
XmiI GTMKAC 1 cut(s) 1443
XmnI GAANNNNTTC 2 cut(s) 1075, 1285
XspI CTAG 6 cut(s) 425, 914, 1002, 1049, 1122, 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.