Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004399

Physical Location & Seq

Genomic Coordinates Forward (+)

30578465 .. 30581119

Gene Structure 2655 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1226200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2655 bp

ATGTACATCAAAACACCAATTCTTCTATTTCTCGCCTTTGCATCCGTGTTCTTGAACGGCCTCGATGCTGCAAAACCGGGCTCAGATCCGCTGATCCTTGGCTGTGGCGCTGATAATGAGGCCAAAGATGCAGATGGGAGGAAATGGCAACCGGACAAGAAGTACCTTGCCGATTCATCAAAGACAACCTCGGCACAAGCGCAGTACCAGGACCCTGCGCTTCTTTCCGAGGTTCCATACATGAAAGCCAGGATTTTCACCGCGAATACCACGTACAAGTTGCCTGTGCAACCCAAGCAGCGCTACATGCTTAGACTTTATTTCTACCCGGCTGTGTATGGCAGCAACAACGCTGAGGGGTCCTATTTTTCGGTTGTTGCCAATGGAATCACTCTCTTGCAAAACTTTAGCGCTTCAATCACTTGCAGGGCCCTTACACAAGCTTATATCATGAGAGAGTATATGTTGGCACCCCTCAACAAGGATTCCCTAGATGTCACCTTTTCGCCTTCCTCGGGGTTTGCATTTGTGAATGGGATTGAGCTGATTGGAGCCCCTGACATGTTTGGCGACGCTGCAATAGTCGGGTCTTCAGACCAGACTTTTGACGGCAAGAGTTCAAATTTGCAGACCATGTTTAGAGTGAATATTGGGGGGCAGTTTATTTCCCCAACCAATGACTCCGGCACCCTAACAAGGACATGGTACGATGACTTTGCTTATGTATATGGTGCACAACTTGGGGTCACCAACGAGGCCGCCAAGAACGTAAAAATTGACTACAAAGATATGCCACCGTACATTGCTCCCGTTGATATCTACAGGACCTCGAGATCAATGGGTGAGAAGAAGGACATCAACCTCGGTTATAATCTCACGTGGGTGTTCAACCAAGTCGATGCTAAATTCATGTACCTTGTGAGGTTACACTTTTGTGACTTTTACCTCACAAAAGCCAATCAAATAGTGTTCACAATCTACATTAACAATCAAACCGCCGAGGCTGAAGCAGATGTGATCGGATGGACAGGAGGAAAGGGGGTGACTACATACAGAGATTATGTGGTAGCGGCCAATGAGGGTTTTAATGGTGACCTCTGGCTAGCTTTGCACCCTTCCAAGAAATCAAGTCCTGAGTTCTACGATTCACTCCTCAATGGGGTGGAGATATTCAAGCTCGAACAGGGTAAAAACTTGGCAGGCCCTAATCCCAAGATTTCTGACATGTTGGCGAAGGACGAGCAAGAGAAGAGAAGCTTTCAAAGTCAGCAGCAGCAGGGAGGAAGCGGTACCAACAAAGCTCACGTGATTGGCGGAGCTGCTGGAGGAGCTGCAGCCTTTGGCATAGTTGCTGCATTGTGCATTGCTGTGTACCAACGAAAGAAAAGGGCCCCGGGAAGTGATGCGCACACTACTAGCTGGCTACCAATCTACGGAAATTCCCATACCAGTGGCACCAAATCAACAATCTCAGGCAAGAGCAATGCTAGCACCCACTTGTCATCAGCTGCTCAAGGCCGTTGCAGGCGTTTCACCTTCCCTGAGATGAAACATGCTACTAAGAATTTTGACGAGTCTAATGTCATTGGTGTTGGAGGATTCGGAAAGGTGTACAAGGGTGTCATCGATGGAGGAACGAAAGTGGCGATCAAGAGATCTAACCCACAATCAGAGCAAGGAGTTAACGAATTCCAGACCGAAATTGAGATGCTTTCAGAGCTGAGGCACAAACATTTGGTCTCGCTGATTGGTTTCTGTGAAGAAGATAGTGAAATGTGCTTGGTGTATGACTACATGTCACGCGGCACAATGCGAGAGCATCTGTACAAGGGCAACAAATCTCAAGGTCAACTGTCATGGAAGAAAAGGCTGGAGATCTGTATTGGAGCAGCAAAGGGACTTCACTACCTTCACACTGGCGCTAGGTGGACCATCATCCACAGGGATGTCAAGACGACAAACATTCTCTTGGACGAGAACTGGGAGGCCAAGGTTTCTGATTTCGGGCTCTCGAAGACTGGTCCCAACATGGATCAAGGCCATGTTAGTACAGTGGTGAAGGGTAGCTTCGGATACTTAGATCCCGAATACTTCAGGAGGCAACAACTGACAGAAAAATCTGATGTCTACTCGTTCGGAGTTGTCCTCTTCGAGGCGTTGTTTGCAAGGCCGGCTCTCAACCCTAGCCTGTCGAAGGAACAGGTCAGTCTAGCAGATTGGGCTCTACATTGTCAAAGAAAAGGAATTGTGGAGGAAAACATTGATCCTCACCTCAAGGGGAAAGTCAACATGGAAAGCGTCAAGAAATTCGCAGACACAGCTGAGAAGTGCTTGTCCGAATCAGGAATCGACCGCCCCACTTTGAACGACATCTTGTGGAACCTCGAGTTTGCGCTCCAGTTGCAAGACGACACAGATGGCTCGAGTCACTCCTCAAGACGCGAGAGAAGTGACTCTGACGACCCCCATCTGAGAAACCAGAACATGATGGCAATACACTACAGCAACCTGAGCCTCGGAAGCGAGAGTGATTTGGTTGAAGAATCCAACACCAACCCCAACAACAACAACAACAACAACAACACCGCCACAAATGAGAACACCGGCGACACCAGCGCAATCTTCTCACAGATTGTCAAGCCAGAAGGTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016324 GO:0030427 GO:0035838 GO:0042995 GO:0044425 GO:0044459 GO:0044463 GO:0044464 GO:0045177 GO:0051286 GO:0071944 GO:0090404 GO:0090406 GO:0098590 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

885

Amino Acids

97.82

Weight (kDa)

6.37

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	34 - 392	3.2e-29	Malectin-like domain
Malectin	PF11721	35 - 169	1e-06	Malectin domain
Malectin	PF11721	213 - 354	2.8e-08	Malectin domain
Pkinase	PF00069	525 - 792	8e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	527 - 792	5.8e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004399)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04690 AT5G28680 AT5G28680
fragaria_vesca	FvH4_4g19690 FvH4_6g02670
malus_domestica	MD04G1226200.v1.1 MD12G1243300.v1.1 MD13G1154700.v1.1
prunus_persica	Prupe.1G117700_v2.0.a1 Prupe.6G345000_v2.0.a1
pyrus_communis	pycom04g19940 pycom13g13150
rosa_chinensis	RchiOBHm_Chr3g0450621 RchiOBHm_Chr4g0425141
rosa_laevigata	RLG00000007395 RLG00000025717
rosa_multiflora	Rmu_sc0000078.1_g000041 Rmu_ssc0000420.1_g000041
rosa_roxburghii	Rroxscaffold_5G00367230 Rroxscaffold_6G00428300
rosa_rugosa	Rorug02G0629100 Rorug04G0201800
rosa_samantha	Rh3AG029800 Rh3BG030200 Rh3CG029100 Rh3DG029900 Rh4AG258400 Rh4BG264200 Rh4CG275300 Rh4DG260300
rosa_wichuraiana	Rw3G002300 Rw4G022370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	870
Acc16I	TGCGCA	1 cut(s)	1407
Acc65I	GGTACC	1 cut(s)	1289
AccB1I	GGYRCC	4 cut(s)	469, 686, 1289, 1454
AccI	GTMKAC	1 cut(s)	2127
AccII	CGCG	3 cut(s)	263, 1803, 2442
AclWI	GGATC	5 cut(s)	80, 88, 2040, 2075, 2258
AcoI	YGGCCR	1 cut(s)	1071
AcsI	RAATTY	6 cut(s)	622, 905, 1438, 1564, 1688, 2306
AcuI	CTGAAG	3 cut(s)	576, 1026, 2077
AcvI	CACGTG	2 cut(s)	879, 1306
AfeI	AGCGCT	2 cut(s)	302, 412
AfiI	CCNNNNNNNGG	7 cut(s)	481, 515, 696, 1159, 1433, 2152, 2194
AflIII	ACRYGT	3 cut(s)	561, 1224, 1794
AgsI	TTSAA	8 cut(s)	55, 417, 621, 889, 1174, 1262, 2365, 2540
AhdI	GACNNNNNGTC	1 cut(s)	1795
AjnI	CCWGG	2 cut(s)	207, 248
AleI	CACNNNNGTG	2 cut(s)	881, 933
Alw21I	GWGCWC	1 cut(s)	736
Alw26I	GTCTC	1 cut(s)	1744
Alw44I	GTGCAC	1 cut(s)	732
AlwI	GGATC	5 cut(s)	80, 88, 2040, 2075, 2258
Ama87I	CYCGRG	5 cut(s)	514, 829, 1393, 2384, 2422
Aor51HI	AGCGCT	2 cut(s)	302, 412
ApaI	GGGCCC	2 cut(s)	433, 1393
ApaLI	GTGCAC	1 cut(s)	732
ApoI	RAATTY	6 cut(s)	622, 905, 1438, 1564, 1688, 2306
ArsI	GACNNNNNNTTYG	4 cut(s)	587, 619, 1722, 1754
Asp718I	GGTACC	1 cut(s)	1289
AspLEI	GCGC	9 cut(s)	110, 202, 220, 303, 413, 1408, 1922, 2395, 2618
AsuC2I	CCSGG	4 cut(s)	78, 329, 1394, 1395
AsuHPI	GGTGA	9 cut(s)	250, 490, 739, 854, 1054, 1103, 1525, 2068, 2261
AsuNHI	GCTAGC	2 cut(s)	1102, 1487
AvaI	CYCGRG	5 cut(s)	514, 829, 1393, 2384, 2422
AvaII	GGWCC	5 cut(s)	211, 360, 825, 1929, 2021
BaeGI	GKGCMC	3 cut(s)	433, 736, 1393
BanI	GGYRCC	4 cut(s)	469, 686, 1289, 1454
BanII	GRGCYC	6 cut(s)	83, 433, 556, 1393, 2010, 2224
BbrPI	CACGTG	2 cut(s)	879, 1306
BbsI	GAAGAC	2 cut(s)	582, 2021
Bbv12I	GWGCWC	1 cut(s)	736
BbvCI	CCTCAGC	2 cut(s)	354, 1721
BccI	CCATC	7 cut(s)	128, 1017, 1622, 1940, 2411, 2475, 2482
BceAI	ACGGC	3 cut(s)	73, 625, 1503
BcgI	CGANNNNNNTGC	2 cut(s)	698, 732
BciT130I	CCWGG	2 cut(s)	209, 250
BciVI	GTATCC	1 cut(s)	2066
BcnI	CCSGG	4 cut(s)	78, 329, 1394, 1395
BcoDI	GTCTC	1 cut(s)	1744
BfaI	CTAG	7 cut(s)	491, 1103, 1416, 1488, 1923, 2184, 2210
BfmI	CTRYAG	3 cut(s)	820, 1332, 2500
BfoI	RGCGCY	4 cut(s)	111, 304, 414, 1923
BfuI	GTATCC	1 cut(s)	2066
BglII	AGATCT	2 cut(s)	1655, 1875
Bme1390I	CCNGG	6 cut(s)	78, 209, 250, 329, 1394, 1395
Bme18I	GGWCC	5 cut(s)	211, 360, 825, 1929, 2021
BmeRI	GACNNNNNGTC	1 cut(s)	1795
BmeT110I	CYCGRG	5 cut(s)	514, 829, 1393, 2384, 2422
BmrFI	CCNGG	6 cut(s)	78, 209, 250, 329, 1394, 1395
BmrI	ACTGGG	1 cut(s)	1990
BmsI	GCATC	7 cut(s)	50, 55, 118, 889, 1393, 1698, 1828
BmtI	GCTAGC	2 cut(s)	1106, 1491
BmuI	ACTGGG	1 cut(s)	1990
BpiI	GAAGAC	2 cut(s)	582, 2021
BplI	GAGNNNNNCTC	2 cut(s)	2130, 2162
BpmI	CTGGAG	3 cut(s)	1344, 1892, 2381
Bpu10I	CCTNAGC	3 cut(s)	354, 1721, 2510
BpuEI	CTTGAG	4 cut(s)	1497, 1827, 2258, 2419
BpuMI	CCSGG	4 cut(s)	78, 329, 1394, 1395
Bsa29I	ATCGAT	1 cut(s)	1626
BsaAI	YACGTR	3 cut(s)	273, 879, 1306
BsaI	GGTCTC	1 cut(s)	1744
BsaWI	WCCGGW	1 cut(s)	151
Bsc4I	CCNNNNNNNGG	7 cut(s)	481, 515, 696, 1159, 1433, 2152, 2194
Bse118I	RCCGGY	2 cut(s)	2170, 2603
Bse1I	ACTGG	5 cut(s)	1449, 1921, 1985, 2023, 2398
Bse3DI	GCAATG	3 cut(s)	801, 1362, 1489
BseBI	CCWGG	2 cut(s)	209, 250
BseCI	ATCGAT	1 cut(s)	1626
BseGI	GGATG	4 cut(s)	41, 1028, 1935, 1951
BseLI	CCNNNNNNNGG	7 cut(s)	481, 515, 696, 1159, 1433, 2152, 2194
BseMI	GCAATG	3 cut(s)	801, 1362, 1489
BseMII	CTCAG	9 cut(s)	96, 345, 1125, 1485, 1533, 1712, 2313, 2462, 2501
BseNI	ACTGG	5 cut(s)	1449, 1921, 1985, 2023, 2398
BseRI	GAGGAG	3 cut(s)	1142, 1341, 2422
BseSI	GKGCMC	3 cut(s)	433, 736, 1393
Bsh1236I	CGCG	3 cut(s)	263, 1803, 2442
Bsh1285I	CGRYCG	1 cut(s)	2353
BshNI	GGYRCC	4 cut(s)	469, 686, 1289, 1454
BshVI	ATCGAT	1 cut(s)	1626
BsiEI	CGRYCG	1 cut(s)	2353
BsiHKAI	GWGCWC	1 cut(s)	736
BsiHKCI	CYCGRG	5 cut(s)	514, 829, 1393, 2384, 2422
BsiSI	CCGG	7 cut(s)	77, 152, 329, 684, 1394, 2171, 2604
BslFI	GGGAC	2 cut(s)	1911, 2007
BslI	CCNNNNNNNGG	7 cut(s)	481, 515, 696, 1159, 1433, 2152, 2194
BsmAI	GTCTC	1 cut(s)	1744
BsmFI	GGGAC	2 cut(s)	1911, 2007
Bso31I	GGTCTC	1 cut(s)	1744
BsoBI	CYCGRG	5 cut(s)	514, 829, 1393, 2384, 2422
Bsp120I	GGGCCC	2 cut(s)	429, 1389
Bsp1286I	GDGCHC	7 cut(s)	83, 433, 556, 736, 1393, 2010, 2224
Bsp1407I	TGTACA	3 cut(s)	3, 1611, 1824
BspCNI	CTCAG	9 cut(s)	95, 346, 1126, 1484, 1534, 1713, 2314, 2463, 2502
BspDI	ATCGAT	1 cut(s)	1626
BspFNI	CGCG	3 cut(s)	263, 1803, 2442
BspHI	TCATGA	1 cut(s)	450
BspMAI	CTGCAG	1 cut(s)	1336
BspOI	GCTAGC	2 cut(s)	1106, 1491
BspPI	GGATC	5 cut(s)	80, 88, 2040, 2075, 2258
BspT107I	GGYRCC	4 cut(s)	469, 686, 1289, 1454
BspTNI	GGTCTC	1 cut(s)	1744
BsrDI	GCAATG	3 cut(s)	801, 1362, 1489
BsrFI	RCCGGY	2 cut(s)	2170, 2603
BsrGI	TGTACA	3 cut(s)	3, 1611, 1824
BsrI	ACTGG	5 cut(s)	1449, 1921, 1985, 2023, 2398
BssAI	RCCGGY	2 cut(s)	2170, 2603
BssT1I	CCWWGG	2 cut(s)	97, 1989
Bst2UI	CCWGG	2 cut(s)	209, 250
Bst4CI	ACNGT	3 cut(s)	798, 1854, 2053
Bst6I	CTCTTC	2 cut(s)	1244, 2153
BstAUI	TGTACA	3 cut(s)	3, 1611, 1824
BstBAI	YACGTR	3 cut(s)	273, 879, 1306
BstC8I	GCNNGC	6 cut(s)	1104, 1201, 1421, 1489, 1526, 2172
BstEII	GGTNACC	2 cut(s)	745, 1091
BstENI	CCTNNNNNAGG	3 cut(s)	479, 2150, 2192
BstF5I	GGATG	4 cut(s)	41, 1028, 1935, 1951
BstFNI	CGCG	3 cut(s)	263, 1803, 2442
BstH2I	RGCGCY	4 cut(s)	111, 304, 414, 1923
BstHHI	GCGC	9 cut(s)	110, 202, 220, 303, 413, 1408, 1922, 2395, 2618
BstMAI	GTCTC	1 cut(s)	1744
BstMCI	CGRYCG	1 cut(s)	2353
BstNI	CCWGG	2 cut(s)	209, 250
BstNSI	RCATGY	5 cut(s)	310, 565, 1228, 1556, 1798
BstPI	GGTNACC	2 cut(s)	745, 1091
BstSCI	CCNGG	6 cut(s)	76, 207, 248, 327, 1392, 1393
BstSFI	CTRYAG	3 cut(s)	820, 1332, 2500
BstSLI	GKGCMC	3 cut(s)	433, 736, 1393
BstUI	CGCG	3 cut(s)	263, 1803, 2442
BstV2I	GAAGAC	2 cut(s)	582, 2021
BstX2I	RGATCY	4 cut(s)	85, 1655, 1875, 2080
BstXI	CCANNNNNNTGG	1 cut(s)	1451
BstYI	RGATCY	4 cut(s)	85, 1655, 1875, 2080
Bsu15I	ATCGAT	1 cut(s)	1626
BsuI	GTATCC	1 cut(s)	2066
BsuTUI	ATCGAT	1 cut(s)	1626
BtsCI	GGATG	4 cut(s)	41, 1028, 1935, 1951
BtsIMutI	CAGTG	3 cut(s)	1456, 1914, 2058
Cac8I	GCNNGC	6 cut(s)	1104, 1201, 1421, 1489, 1526, 2172
CciI	TCATGA	1 cut(s)	450
CfoI	GCGC	9 cut(s)	110, 202, 220, 303, 413, 1408, 1922, 2395, 2618
Cfr10I	RCCGGY	2 cut(s)	2170, 2603
Cfr9I	CCCGGG	1 cut(s)	1393
ClaI	ATCGAT	1 cut(s)	1626
CseI	GACGC	3 cut(s)	581, 2287, 2448
DriI	GACNNNNNGTC	1 cut(s)	1795
EaeI	YGGCCR	1 cut(s)	1071
Eam1104I	CTCTTC	2 cut(s)	1244, 2153
Eam1105I	GACNNNNNGTC	1 cut(s)	1795
EarI	CTCTTC	2 cut(s)	1244, 2153
EciI	GGCGGA	1 cut(s)	1329
Eco130I	CCWWGG	2 cut(s)	97, 1989
Eco24I	GRGCYC	6 cut(s)	83, 433, 556, 1393, 2010, 2224
Eco31I	GGTCTC	1 cut(s)	1744
Eco32I	GATATC	1 cut(s)	817
Eco47I	GGWCC	5 cut(s)	211, 360, 825, 1929, 2021
Eco47III	AGCGCT	2 cut(s)	302, 412
Eco57I	CTGAAG	3 cut(s)	576, 1026, 2077
Eco72I	CACGTG	2 cut(s)	879, 1306
Eco88I	CYCGRG	5 cut(s)	514, 829, 1393, 2384, 2422
Eco91I	GGTNACC	2 cut(s)	745, 1091
EcoNI	CCTNNNNNAGG	3 cut(s)	479, 2150, 2192
EcoO109I	RGGNCCY	8 cut(s)	211, 360, 429, 430, 825, 1202, 1389, 1390
EcoO65I	GGTNACC	2 cut(s)	745, 1091
EcoRI	GAATTC	1 cut(s)	1688
EcoRII	CCWGG	2 cut(s)	207, 248
EcoRV	GATATC	1 cut(s)	817
EcoT14I	CCWWGG	2 cut(s)	97, 1989
EcoT38I	GRGCYC	6 cut(s)	83, 433, 556, 1393, 2010, 2224
ErhI	CCWWGG	2 cut(s)	97, 1989
FalI	AAGNNNNNCTT	4 cut(s)	1241, 1273, 2051, 2083
FaqI	GGGAC	2 cut(s)	1911, 2007
FblI	GTMKAC	1 cut(s)	2127
FokI	GGATG	4 cut(s)	28, 1035, 1922, 1958
FriOI	GRGCYC	6 cut(s)	83, 433, 556, 1393, 2010, 2224
FspAI	RTGCGCAY	1 cut(s)	1407
FspBI	CTAG	7 cut(s)	491, 1103, 1416, 1488, 1923, 2184, 2210
FspI	TGCGCA	1 cut(s)	1407
GlaI	GCGC	9 cut(s)	109, 201, 219, 302, 412, 1407, 1921, 2394, 2617
GsuI	CTGGAG	3 cut(s)	1344, 1892, 2381
HaeII	RGCGCY	4 cut(s)	111, 304, 414, 1923
HapII	CCGG	7 cut(s)	77, 152, 329, 684, 1394, 2171, 2604
HgaI	GACGC	3 cut(s)	581, 2287, 2448
HhaI	GCGC	9 cut(s)	110, 202, 220, 303, 413, 1408, 1922, 2395, 2618
Hin6I	GCGC	9 cut(s)	108, 200, 218, 301, 411, 1406, 1920, 2393, 2616
HinP1I	GCGC	9 cut(s)	108, 200, 218, 301, 411, 1406, 1920, 2393, 2616
HincII	GTYRAC	3 cut(s)	1684, 1850, 2287
HindII	GTYRAC	3 cut(s)	1684, 1850, 2287
HindIII	AAGCTT	2 cut(s)	441, 1255
HpaI	GTTAAC	1 cut(s)	1684
HpaII	CCGG	7 cut(s)	77, 152, 329, 684, 1394, 2171, 2604
HphI	GGTGA	9 cut(s)	250, 490, 739, 854, 1054, 1103, 1525, 2068, 2261
Hpy166II	GTNNAC	9 cut(s)	734, 972, 1372, 1612, 1684, 1850, 1929, 2128, 2287
Hpy8I	GTNNAC	9 cut(s)	734, 972, 1372, 1612, 1684, 1850, 1929, 2128, 2287
Hpy99I	CGWCG	1 cut(s)	575
HpyAV	CCTTC	9 cut(s)	519, 844, 1125, 1228, 1546, 1919, 2053, 2188, 2639
HpyCH4III	ACNGT	3 cut(s)	798, 1854, 2053
HpyCH4IV	ACGT	4 cut(s)	272, 768, 878, 1305
HpySE526I	ACGT	4 cut(s)	272, 768, 878, 1305
HspAI	GCGC	9 cut(s)	108, 200, 218, 301, 411, 1406, 1920, 2393, 2616
KpnI	GGTACC	1 cut(s)	1293
KroI	GCCGGC	1 cut(s)	2170
KroNI	GCCGGC	1 cut(s)	2172
KspAI	GTTAAC	1 cut(s)	1684
LmnI	GCTCC	6 cut(s)	551, 811, 1316, 1328, 1886, 2400
LweI	GCATC	7 cut(s)	50, 55, 118, 889, 1393, 1698, 1828
MaeI	CTAG	7 cut(s)	491, 1103, 1416, 1488, 1923, 2184, 2210
MaeII	ACGT	4 cut(s)	272, 768, 878, 1305
MaeIII	GTNAC	9 cut(s)	496, 745, 924, 935, 1042, 1091, 1797, 2426, 2450
MflI	RGATCY	4 cut(s)	85, 1655, 1875, 2080
MhlI	GDGCHC	7 cut(s)	83, 433, 556, 736, 1393, 2010, 2224
MlyI	GAGTC	4 cut(s)	674, 1583, 2434, 2447
MmeI	TCCRAC	2 cut(s)	1573, 2571
MroNI	GCCGGC	1 cut(s)	2170
MseI	TTAA	3 cut(s)	984, 1086, 1683
MslI	CAYNNNNRTG	5 cut(s)	881, 933, 1367, 1449, 1944
MspA1I	CMGCKG	3 cut(s)	91, 1508, 2321
MspI	CCGG	7 cut(s)	77, 152, 329, 684, 1394, 2171, 2604
MspR9I	CCNGG	6 cut(s)	78, 209, 250, 329, 1394, 1395
MvaI	CCWGG	2 cut(s)	209, 250
MvnI	CGCG	3 cut(s)	263, 1803, 2442
NaeI	GCCGGC	1 cut(s)	2172
NciI	CCSGG	4 cut(s)	78, 329, 1394, 1395
NgoMIV	GCCGGC	1 cut(s)	2170
NheI	GCTAGC	2 cut(s)	1102, 1487
NmeAIII	GCCGAG	2 cut(s)	170, 1024
NmuCI	GTSAC	8 cut(s)	496, 745, 935, 1042, 1091, 1797, 2426, 2450
NsbI	TGCGCA	1 cut(s)	1407
NspI	RCATGY	5 cut(s)	310, 565, 1228, 1556, 1798
OliI	CACNNNNGTG	2 cut(s)	881, 933
PaeR7I	CTCGAG	3 cut(s)	829, 2384, 2422
PagI	TCATGA	1 cut(s)	450
PciI	ACATGT	3 cut(s)	561, 1224, 1794
PcsI	WCGNNNNNNNCGW	1 cut(s)	1643
PdiI	GCCGGC	1 cut(s)	2172
PfeI	GAWTC	8 cut(s)	173, 387, 485, 1145, 1599, 2339, 2346, 2543
PleI	GAGTC	4 cut(s)	674, 1582, 2433, 2447
PmaCI	CACGTG	2 cut(s)	879, 1306
PmlI	CACGTG	2 cut(s)	879, 1306
PpsI	GAGTC	4 cut(s)	674, 1582, 2433, 2447
Ppu21I	YACGTR	3 cut(s)	273, 879, 1306
PpuMI	RGGWCCY	3 cut(s)	211, 360, 825
PscI	ACATGT	3 cut(s)	561, 1224, 1794
PsiI	TTATAA	1 cut(s)	870
Psp5II	RGGWCCY	3 cut(s)	211, 360, 825
Psp6I	CCWGG	2 cut(s)	207, 248
PspCI	CACGTG	2 cut(s)	879, 1306
PspEI	GGTNACC	2 cut(s)	745, 1091
PspGI	CCWGG	2 cut(s)	207, 248
PspOMI	GGGCCC	2 cut(s)	429, 1389
PspPPI	RGGWCCY	3 cut(s)	211, 360, 825
PspXI	VCTCGAGB	2 cut(s)	2384, 2422
PstI	CTGCAG	1 cut(s)	1336
PsuI	RGATCY	4 cut(s)	85, 1655, 1875, 2080
PvuII	CAGCTG	2 cut(s)	1508, 2321
RseI	CAYNNNNRTG	5 cut(s)	881, 933, 1367, 1449, 1944
SaqAI	TTAA	3 cut(s)	984, 1086, 1683
SchI	GAGTC	4 cut(s)	674, 1583, 2434, 2447
ScrFI	CCNGG	6 cut(s)	78, 209, 250, 329, 1394, 1395
SduI	GDGCHC	7 cut(s)	83, 433, 556, 736, 1393, 2010, 2224
SfaNI	GCATC	7 cut(s)	50, 55, 118, 889, 1393, 1698, 1828
SfcI	CTRYAG	3 cut(s)	820, 1332, 2500
Sfr274I	CTCGAG	3 cut(s)	829, 2384, 2422
SgrAI	CRCCGGYG	1 cut(s)	2603
SinI	GGWCC	5 cut(s)	211, 360, 825, 1929, 2021
SlaI	CTCGAG	3 cut(s)	829, 2384, 2422
SmaI	CCCGGG	1 cut(s)	1395
SmiMI	CAYNNNNRTG	5 cut(s)	881, 933, 1367, 1449, 1944
SmlI	CTYRAG	7 cut(s)	829, 1512, 1842, 2273, 2384, 2422, 2434
SmoI	CTYRAG	7 cut(s)	829, 1512, 1842, 2273, 2384, 2422, 2434
SspI	AATATT	1 cut(s)	649
SspMI	CTAG	7 cut(s)	491, 1103, 1416, 1488, 1923, 2184, 2210
StyD4I	CCNGG	6 cut(s)	76, 207, 248, 327, 1392, 1393
StyI	CCWWGG	2 cut(s)	97, 1989
TaaI	ACNGT	3 cut(s)	798, 1854, 2053
TaiI	ACGT	4 cut(s)	275, 771, 881, 1308
TaqII	GACCGA	1 cut(s)	1712
TatI	WGTACW	4 cut(s)	3, 1611, 1824, 2048
TauI	GCSGC	3 cut(s)	761, 1073, 1806
TfiI	GAWTC	8 cut(s)	173, 387, 485, 1145, 1599, 2339, 2346, 2543
Tru1I	TTAA	3 cut(s)	984, 1086, 1683
Tru9I	TTAA	3 cut(s)	984, 1086, 1683
TscAI	CASTG	3 cut(s)	1456, 1921, 2058
TseFI	GTSAC	8 cut(s)	496, 745, 935, 1042, 1091, 1797, 2426, 2450
Tsp45I	GTSAC	8 cut(s)	496, 745, 935, 1042, 1091, 1797, 2426, 2450
TspDTI	ATGAA	4 cut(s)	165, 257, 898, 1562
TspGWI	ACGGA	2 cut(s)	34, 1449
TspMI	CCCGGG	1 cut(s)	1393
TspRI	CASTG	3 cut(s)	1456, 1921, 2058
VneI	GTGCAC	1 cut(s)	732
VpaK11BI	GGWCC	5 cut(s)	211, 360, 825, 1929, 2021
XagI	CCTNNNNNAGG	3 cut(s)	479, 2150, 2192
XapI	RAATTY	6 cut(s)	622, 905, 1438, 1564, 1688, 2306
XceI	RCATGY	5 cut(s)	310, 565, 1228, 1556, 1798
XhoI	CTCGAG	3 cut(s)	829, 2384, 2422
XmaI	CCCGGG	1 cut(s)	1393
XmiI	GTMKAC	1 cut(s)	2127
XspI	CTAG	7 cut(s)	491, 1103, 1416, 1488, 1923, 2184, 2210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.