Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004399

Physical Location & Seq

Genomic Coordinates Forward (+)

31399025 .. 31401664

Gene Structure 2640 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1243300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGTACATCAAAACCCCGATTCTTCTCTTTCTCGCCTTTGCATCCGTGTTCTTGAACGGCCTCAATGCTGAAAACCCGGGCTCAGATCCGCTGATCCTTGGCTGCGGCGCTGAAAATGAGGCCAAAGATGCAGATGGGAGGAAATGGCAACCGGACTCGAAGTACCTTGCCGATTCATCAAAGACAACCTCGGCACAAGCGCAGTACCAGGACCCTGCGCTTCTTTCCGAGGTTCCATACATGAAAGCCAGGATTTTCACCGCGAATGCCACATACAAGTTGCCTGTGAACCCGAAGCAGCGGTACATGCTTAGACTTTATTTCTATCCGGCTGTGTACGGCAGCAACAACGCTGAGGGGTCCTATTTTTCGGTTGTCGCCAATGGAATCACTCTCTTGCAAAACTTTAGCGCTTCAATCACTTGCAAGGCCCTTACACAAGCTTATATCATGAGAGAGTATCTGCTGGCACCCCTCAACAAGGATTCTCTAGAGGTCACCTTTTCGCCTTCCTCGGGGTTTGCATTTGTGAATGGCATTGAGCTGATTGGAACGCCTGACATGTTCGGCGACGCTGCAATAGTCGGGTCTTCAGACCAGACTTTTGACGGCAAGAGGTCAAATTTGCAGACCATGTTTAGAGTGAATATTGGGGGGCAGTTTATTTCCCCAACCAATGACTCCGGCGCCCTAACAAGGACATGGTACGATGACTTTGCTTATGTATATGGTGCGCAACTTGGGGTCACCAATGAGGCCGCCAAGGGTGTAAAAATCGACTACAAAAATATGCCACCATACATTGCTCCCGTTGATATCTACAGGACCTCGAGATCAATGGGTGAGAAGAAGGACATCAACCTCGGTTACAACCTCACATGGGTGTTCAACCAAGTTGATGCCAAATTCATGTACCTTGTGAGGTTACACTTCTGTGACTTTTACCTCACCAAAACCAACCAAATAGTGTTCACAATCTACATTAATAATCAAACCGCCGAGGCTGAAGCAGATGTGATCGGATGGACAGGAGGAAAGGGGGTGACTACATACAGAGATTATGTGGTAGCGGCCGATGAGGATTTTAACGGTGACCTTTGGCTAGCCTTGCATCCTTCAACGAAATCAAGTCCAGAGTTCTATGATTCGCTCCTCAATGGGGTGGAGATATTCAAGCTCGAACAAGCTAAAAACTTGGCAGGCCTTAATCCCAAGATTTCCAACATGTTGGCAAAGGATATTGAGGAAAAGCAGAGAAGCTTTCAAAGTCAGCAGCAACAGGGAGAAAGCGGAACCAACAAAGCTCACGTGATTGGTGGTGCTGCTGGAGGAGCTGCAGCCTTTGGCATAGTTGCTGCATTGTGCATTGCTGTGTACCAACGAAAGAAAAAGGCCCTGGGAAGCGATACACACACCTCTAGCTGGCTACCAATCTACGGCAATTCCCATACCAGTGGCACCAAATCCACAACCTCAGGCAAGAGCAATGGTAGCGCCCACCTGTCATCAGCTGCTCAAGGCCGTTGTAGGCGTTTCACCTTCCCTGAGATGAAACATGCTACTAAGAATTTCGACGAGTCTAATGTCATTGGTGTTGGAGGATTCGGAAAGGTGTACAAGGGTGCCATTGATGGAGGAACAAATGTGGCCATCAAGAGATCCAACCCACAATCAGAGCAAGGAGTTCACGAATTCCAGACCGAAATTGAGATGCTTTCAGAGCTGAGGCATAAACACTTGGTCTCTCTAATTGGCTTCTGTGAAGAAGATAATGAAATGTGCTTGGTTTATGACTACATGTCACGCGGCACTCTACGAGAGCATCTGTACAAGGGCAACAAATCTCAAGGTCAACTGTCATGGAAGAAAAGGCTGGAGATCTGTATTGGAGCAGCAAAGGGACTTCATTACCTTCACACCGGGGCTAGGTGGACCATCATCCACAGGGATGTCAAGACGACAAACATTCTCTTGGACGAGAATTGGGAGGCCAAGGTTTCTGATTTCGGGCTCTCGAAGACTGGTCCCAACATGGATCAAGGCCATGTTAGCACAGTGGTGAAGGGTAGCTTTGGTTACTTGGACCCCGAATACTTCAGGAGGCAGCAACTGACAGAAAAATCCGATGTCTACTCGTTCGGAGTTGTCCTCTTCGAGGCATTGTTCGCAAGGCCAGCTCTCAACCCTAGCCTGTCGAAGGAACAGGTCAGTCTAGCGGATTGGGCTCTTCATTGCCAAAGAAAAGGAATTGTGGAGGAAAACATTGATCCTCACCTCAAGGGGAAAGTCAACATGGAAAGCGTAAAAAAATTCGCAGACACAGCTGAGAAGTGCTTGTCCGACTCAGGACTCGACCGCCCCACTTTGAACGACATCTTGTGGAACCTTGAGTTTGCACTCCAGTTGCAGGACGACACAGATGGCTCGAGTCACTCCTCAAGACGCGAAAGAAGTGACTCTGACGACCCCCATCTGAGAAACCAAAACATGATGGCAGTACACTACAGCAACCTGAGCCTCGGAAGCGATAGTGATTTGGGAGAAGAAACCAACAACAACAACAACACCACCACCACAAATGAGAACACCGACGACACCAGCGCAATCTTCTCACAGATTGTCAATCCAAAAGGTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016324 GO:0030427 GO:0035838 GO:0042995 GO:0044425 GO:0044459 GO:0044463 GO:0044464 GO:0045177 GO:0051286 GO:0071944 GO:0090404 GO:0090406 GO:0098590 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

880

Amino Acids

97.32

Weight (kDa)

6.1

Isoelectric Point (pI)

37.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	34 - 392	6.7e-28	Malectin-like domain
Malectin	PF11721	35 - 169	6.4e-07	Malectin domain
Malectin	PF11721	233 - 354	8.2e-08	Malectin domain
Pkinase	PF00069	526 - 793	3.1e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	528 - 792	6e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004399)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04690 AT5G28680 AT5G28680
fragaria_vesca	FvH4_4g19690 FvH4_6g02670
malus_domestica	MD04G1226200.v1.1 MD12G1243300.v1.1 MD13G1154700.v1.1
prunus_persica	Prupe.1G117700_v2.0.a1 Prupe.6G345000_v2.0.a1
pyrus_communis	pycom04g19940 pycom13g13150
rosa_chinensis	RchiOBHm_Chr3g0450621 RchiOBHm_Chr4g0425141
rosa_laevigata	RLG00000007395 RLG00000025717
rosa_multiflora	Rmu_sc0000078.1_g000041 Rmu_ssc0000420.1_g000041
rosa_roxburghii	Rroxscaffold_5G00367230 Rroxscaffold_6G00428300
rosa_rugosa	Rorug02G0629100 Rorug04G0201800
rosa_samantha	Rh3AG029800 Rh3BG030200 Rh3CG029100 Rh3DG029900 Rh4AG258400 Rh4BG264200 Rh4CG275300 Rh4DG260300
rosa_wichuraiana	Rw3G002300 Rw4G022370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	735
AccB1I	GGYRCC	4 cut(s)	469, 686, 1457, 1622
AccI	GTMKAC	1 cut(s)	2130
AccII	CGCG	3 cut(s)	263, 1806, 2445
AclWI	GGATC	5 cut(s)	80, 88, 1653, 2043, 2261
AcoI	YGGCCR	2 cut(s)	1071, 1647
AcsI	RAATTY	5 cut(s)	622, 905, 1567, 1691, 2309
AcuI	CTGAAG	3 cut(s)	576, 1026, 2080
AcvI	CACGTG	1 cut(s)	1309
AcyI	GRCGYC	1 cut(s)	687
AfeI	AGCGCT	1 cut(s)	412
AflIII	ACRYGT	3 cut(s)	561, 1224, 1797
AgsI	TTSAA	7 cut(s)	55, 417, 889, 1119, 1174, 1265, 2368
AhdI	GACNNNNNGTC	1 cut(s)	1798
AjnI	CCWGG	3 cut(s)	207, 248, 1395
AleI	CACNNNNGTG	3 cut(s)	881, 933, 2057
Alw26I	GTCTC	1 cut(s)	1747
AlwI	GGATC	5 cut(s)	80, 88, 1653, 2043, 2261
AlwNI	CAGNNNCTG	1 cut(s)	2110
Ama87I	CYCGRG	4 cut(s)	76, 514, 829, 2425
Aor51HI	AGCGCT	1 cut(s)	412
ApoI	RAATTY	5 cut(s)	622, 905, 1567, 1691, 2309
ArsI	GACNNNNNNTTYG	2 cut(s)	587, 619
AseI	ATTAAT	1 cut(s)	984
AspLEI	GCGC	8 cut(s)	110, 202, 220, 413, 689, 736, 1496, 2603
AspS9I	GGNCC	8 cut(s)	211, 360, 430, 825, 1393, 1932, 2024, 2083
AsuC2I	CCSGG	3 cut(s)	77, 78, 1921
AsuNHI	GCTAGC	1 cut(s)	1102
AvaI	CYCGRG	4 cut(s)	76, 514, 829, 2425
AvaII	GGWCC	6 cut(s)	211, 360, 825, 1932, 2024, 2083
AxyI	CCTNAGG	1 cut(s)	1474
BalI	TGGCCA	1 cut(s)	1649
BanI	GGYRCC	4 cut(s)	469, 686, 1457, 1622
BanII	GRGCYC	3 cut(s)	83, 2013, 2227
BarI	GAAGNNNNNNTAC	2 cut(s)	287, 319
BbrPI	CACGTG	1 cut(s)	1309
BbsI	GAAGAC	2 cut(s)	582, 2024
BbvCI	CCTCAGC	2 cut(s)	354, 1724
BccI	CCATC	8 cut(s)	128, 1017, 1625, 1658, 1943, 2414, 2478, 2485
BceAI	ACGGC	5 cut(s)	73, 355, 625, 1453, 1506
BcgI	CGANNNNNNTGC	2 cut(s)	698, 732
BciT130I	CCWGG	3 cut(s)	209, 250, 1397
BcnI	CCSGG	3 cut(s)	77, 78, 1921
BcoDI	GTCTC	1 cut(s)	1747
BfaI	CTAG	6 cut(s)	491, 1103, 1419, 1926, 2187, 2213
BfmI	CTRYAG	3 cut(s)	820, 1335, 2503
BfoI	RGCGCY	4 cut(s)	111, 414, 690, 1497
BglII	AGATCT	1 cut(s)	1878
Bme1390I	CCNGG	6 cut(s)	77, 78, 209, 250, 1397, 1921
Bme18I	GGWCC	6 cut(s)	211, 360, 825, 1932, 2024, 2083
BmeRI	GACNNNNNGTC	1 cut(s)	1798
BmeT110I	CYCGRG	4 cut(s)	76, 514, 829, 2425
BmgT120I	GGNCC	8 cut(s)	211, 360, 430, 825, 1393, 1932, 2024, 2083
BmrFI	CCNGG	6 cut(s)	77, 78, 209, 250, 1397, 1921
BmsI	GCATC	6 cut(s)	50, 118, 889, 1120, 1701, 1831
BmtI	GCTAGC	1 cut(s)	1106
BpiI	GAAGAC	2 cut(s)	582, 2024
BplI	GAGNNNNNCTC	2 cut(s)	2133, 2165
BpmI	CTGGAG	3 cut(s)	1347, 1895, 2384
Bpu10I	CCTNAGC	3 cut(s)	354, 1724, 2513
BpuEI	CTTGAG	5 cut(s)	1500, 1830, 2261, 2408, 2422
BpuMI	CCSGG	3 cut(s)	77, 78, 1921
BsaAI	YACGTR	1 cut(s)	1309
BsaHI	GRCGYC	1 cut(s)	687
BsaI	GGTCTC	1 cut(s)	1747
BsaWI	WCCGGW	1 cut(s)	151
Bse1I	ACTGG	3 cut(s)	1452, 2026, 2401
Bse21I	CCTNAGG	1 cut(s)	1474
Bse3DI	GCAATG	4 cut(s)	801, 1365, 1492, 2230
BseBI	CCWGG	3 cut(s)	209, 250, 1397
BseGI	GGATG	5 cut(s)	41, 1028, 1111, 1938, 1954
BseMI	GCAATG	4 cut(s)	801, 1365, 1492, 2230
BseMII	CTCAG	9 cut(s)	96, 345, 1488, 1536, 1715, 2316, 2358, 2465, 2504
BseNI	ACTGG	3 cut(s)	1452, 2026, 2401
BseRI	GAGGAG	3 cut(s)	1142, 1344, 2425
BseX3I	CGGCCG	1 cut(s)	1071
Bsh1236I	CGCG	3 cut(s)	263, 1806, 2445
Bsh1285I	CGRYCG	2 cut(s)	1074, 2356
BshNI	GGYRCC	4 cut(s)	469, 686, 1457, 1622
BsiEI	CGRYCG	2 cut(s)	1074, 2356
BsiHKCI	CYCGRG	4 cut(s)	76, 514, 829, 2425
BsiSI	CCGG	5 cut(s)	77, 152, 329, 684, 1920
BslFI	GGGAC	2 cut(s)	1914, 2010
BsmAI	GTCTC	1 cut(s)	1747
BsmFI	GGGAC	2 cut(s)	1914, 2010
BsmI	GAATGC	1 cut(s)	271
Bso31I	GGTCTC	1 cut(s)	1747
BsoBI	CYCGRG	4 cut(s)	76, 514, 829, 2425
Bsp1286I	GDGCHC	3 cut(s)	83, 2013, 2227
Bsp1407I	TGTACA	3 cut(s)	3, 1614, 1827
Bsp143I	GATC	8 cut(s)	85, 93, 833, 1017, 1658, 1878, 2035, 2266
BspCNI	CTCAG	9 cut(s)	95, 346, 1487, 1537, 1716, 2317, 2357, 2466, 2505
BspFNI	CGCG	3 cut(s)	263, 1806, 2445
BspHI	TCATGA	1 cut(s)	450
BspMAI	CTGCAG	1 cut(s)	1339
BspOI	GCTAGC	1 cut(s)	1106
BspPI	GGATC	5 cut(s)	80, 88, 1653, 2043, 2261
BspQI	GCTCTTC	1 cut(s)	2232
BspT107I	GGYRCC	4 cut(s)	469, 686, 1457, 1622
BspTNI	GGTCTC	1 cut(s)	1747
BsrDI	GCAATG	4 cut(s)	801, 1365, 1492, 2230
BsrGI	TGTACA	3 cut(s)	3, 1614, 1827
BsrI	ACTGG	3 cut(s)	1452, 2026, 2401
BssMI	GATC	8 cut(s)	85, 93, 833, 1017, 1658, 1878, 2035, 2266
BssNI	GRCGYC	1 cut(s)	687
BssT1I	CCWWGG	3 cut(s)	97, 762, 1992
Bst2UI	CCWGG	3 cut(s)	209, 250, 1397
Bst4CI	ACNGT	3 cut(s)	1091, 1857, 2056
Bst6I	CTCTTC	2 cut(s)	2156, 2232
BstACI	GRCGYC	1 cut(s)	687
BstAUI	TGTACA	3 cut(s)	3, 1614, 1827
BstBAI	YACGTR	1 cut(s)	1309
BstC8I	GCNNGC	5 cut(s)	468, 1104, 1201, 1424, 2175
BstEII	GGTNACC	3 cut(s)	496, 745, 1091
BstENI	CCTNNNNNAGG	3 cut(s)	479, 2153, 2195
BstF5I	GGATG	5 cut(s)	41, 1028, 1111, 1938, 1954
BstFNI	CGCG	3 cut(s)	263, 1806, 2445
BstH2I	RGCGCY	4 cut(s)	111, 414, 690, 1497
BstHHI	GCGC	8 cut(s)	110, 202, 220, 413, 689, 736, 1496, 2603
BstKTI	GATC	8 cut(s)	88, 96, 836, 1020, 1661, 1881, 2038, 2269
BstMAI	GTCTC	1 cut(s)	1747
BstMBI	GATC	8 cut(s)	85, 93, 833, 1017, 1658, 1878, 2035, 2266
BstMCI	CGRYCG	2 cut(s)	1074, 2356
BstNI	CCWGG	3 cut(s)	209, 250, 1397
BstNSI	RCATGY	5 cut(s)	310, 565, 1228, 1559, 1801
BstPI	GGTNACC	3 cut(s)	496, 745, 1091
BstSCI	CCNGG	6 cut(s)	75, 76, 207, 248, 1395, 1919
BstSFI	CTRYAG	3 cut(s)	820, 1335, 2503
BstUI	CGCG	3 cut(s)	263, 1806, 2445
BstV2I	GAAGAC	2 cut(s)	582, 2024
BstX2I	RGATCY	3 cut(s)	85, 1658, 1878
BstXI	CCANNNNNNTGG	2 cut(s)	1228, 1454
BstYI	RGATCY	3 cut(s)	85, 1658, 1878
BstZI	CGGCCG	1 cut(s)	1071
Bsu36I	CCTNAGG	1 cut(s)	1474
BtsCI	GGATG	5 cut(s)	41, 1028, 1111, 1938, 1954
BtsIMutI	CAGTG	2 cut(s)	1459, 2061
Cac8I	GCNNGC	5 cut(s)	468, 1104, 1201, 1424, 2175
CaiI	CAGNNNCTG	1 cut(s)	2110
CciI	TCATGA	1 cut(s)	450
CfoI	GCGC	8 cut(s)	110, 202, 220, 413, 689, 736, 1496, 2603
Cfr13I	GGNCC	8 cut(s)	211, 360, 430, 825, 1393, 1932, 2024, 2083
Cfr9I	CCCGGG	1 cut(s)	76
CseI	GACGC	2 cut(s)	581, 2451
DinI	GGCGCC	1 cut(s)	688
DpnI	GATC	8 cut(s)	87, 95, 835, 1019, 1660, 1880, 2037, 2268
DpnII	GATC	8 cut(s)	85, 93, 833, 1017, 1658, 1878, 2035, 2266
DriI	GACNNNNNGTC	1 cut(s)	1798
EaeI	YGGCCR	2 cut(s)	1071, 1647
EagI	CGGCCG	1 cut(s)	1071
Eam1104I	CTCTTC	2 cut(s)	2156, 2232
Eam1105I	GACNNNNNGTC	1 cut(s)	1798
EarI	CTCTTC	2 cut(s)	2156, 2232
EclXI	CGGCCG	1 cut(s)	1071
Eco130I	CCWWGG	3 cut(s)	97, 762, 1992
Eco147I	AGGCCT	1 cut(s)	1203
Eco24I	GRGCYC	3 cut(s)	83, 2013, 2227
Eco31I	GGTCTC	1 cut(s)	1747
Eco32I	GATATC	1 cut(s)	817
Eco47I	GGWCC	6 cut(s)	211, 360, 825, 1932, 2024, 2083
Eco47III	AGCGCT	1 cut(s)	412
Eco52I	CGGCCG	1 cut(s)	1071
Eco57I	CTGAAG	3 cut(s)	576, 1026, 2080
Eco72I	CACGTG	1 cut(s)	1309
Eco81I	CCTNAGG	1 cut(s)	1474
Eco88I	CYCGRG	4 cut(s)	76, 514, 829, 2425
Eco91I	GGTNACC	3 cut(s)	496, 745, 1091
EcoNI	CCTNNNNNAGG	3 cut(s)	479, 2153, 2195
EcoO109I	RGGNCCY	5 cut(s)	211, 360, 430, 825, 1393
EcoO65I	GGTNACC	3 cut(s)	496, 745, 1091
EcoRI	GAATTC	1 cut(s)	1691
EcoRII	CCWGG	3 cut(s)	207, 248, 1395
EcoRV	GATATC	1 cut(s)	817
EcoT14I	CCWWGG	3 cut(s)	97, 762, 1992
EcoT38I	GRGCYC	3 cut(s)	83, 2013, 2227
EgeI	GGCGCC	1 cut(s)	688
EheI	GGCGCC	1 cut(s)	688
ErhI	CCWWGG	3 cut(s)	97, 762, 1992
FalI	AAGNNNNNCTT	2 cut(s)	2054, 2086
FaqI	GGGAC	2 cut(s)	1914, 2010
FblI	GTMKAC	1 cut(s)	2130
FokI	GGATG	5 cut(s)	28, 1035, 1098, 1925, 1961
FriOI	GRGCYC	3 cut(s)	83, 2013, 2227
FspBI	CTAG	6 cut(s)	491, 1103, 1419, 1926, 2187, 2213
FspI	TGCGCA	1 cut(s)	735
GlaI	GCGC	8 cut(s)	109, 201, 219, 412, 688, 735, 1495, 2602
GsuI	CTGGAG	3 cut(s)	1347, 1895, 2384
HaeII	RGCGCY	4 cut(s)	111, 414, 690, 1497
HapII	CCGG	5 cut(s)	77, 152, 329, 684, 1920
HgaI	GACGC	2 cut(s)	581, 2451
HhaI	GCGC	8 cut(s)	110, 202, 220, 413, 689, 736, 1496, 2603
Hin1I	GRCGYC	1 cut(s)	687
Hin6I	GCGC	8 cut(s)	108, 200, 218, 411, 687, 734, 1494, 2601
HinP1I	GCGC	8 cut(s)	108, 200, 218, 411, 687, 734, 1494, 2601
HincII	GTYRAC	2 cut(s)	1853, 2290
HindII	GTYRAC	2 cut(s)	1853, 2290
HindIII	AAGCTT	2 cut(s)	441, 1258
HpaII	CCGG	5 cut(s)	77, 152, 329, 684, 1920
Hpy99I	CGWCG	3 cut(s)	575, 1577, 2594
HpyAV	CCTTC	7 cut(s)	519, 844, 1125, 1549, 1922, 2056, 2191
HpyCH4III	ACNGT	3 cut(s)	1091, 1857, 2056
HpyCH4IV	ACGT	1 cut(s)	1308
HpySE526I	ACGT	1 cut(s)	1308
Hsp92I	GRCGYC	1 cut(s)	687
HspAI	GCGC	8 cut(s)	108, 200, 218, 411, 687, 734, 1494, 2601
KasI	GGCGCC	1 cut(s)	686
Kzo9I	GATC	8 cut(s)	85, 93, 833, 1017, 1658, 1878, 2035, 2266
LguI	GCTCTTC	1 cut(s)	2232
LmnI	GCTCC	4 cut(s)	811, 1155, 1331, 1889
LweI	GCATC	6 cut(s)	50, 118, 889, 1120, 1701, 1831
MaeI	CTAG	6 cut(s)	491, 1103, 1419, 1926, 2187, 2213
MaeII	ACGT	1 cut(s)	1308
MalI	GATC	8 cut(s)	87, 95, 835, 1019, 1660, 1880, 2037, 2268
MboI	GATC	8 cut(s)	85, 93, 833, 1017, 1658, 1878, 2035, 2266
MflI	RGATCY	3 cut(s)	85, 1658, 1878
MhlI	GDGCHC	3 cut(s)	83, 2013, 2227
MlsI	TGGCCA	1 cut(s)	1649
MluNI	TGGCCA	1 cut(s)	1649
Mly113I	GGCGCC	1 cut(s)	687
MlyI	GAGTC	7 cut(s)	149, 674, 1586, 2336, 2343, 2437, 2450
MmeI	TCCRAC	4 cut(s)	1245, 1576, 1686, 2364
Mox20I	TGGCCA	1 cut(s)	1649
MscI	TGGCCA	1 cut(s)	1649
MseI	TTAA	3 cut(s)	984, 1086, 1206
MslI	CAYNNNNRTG	6 cut(s)	881, 933, 1370, 1452, 1947, 2057
Msp20I	TGGCCA	1 cut(s)	1649
MspA1I	CMGCKG	4 cut(s)	91, 301, 1511, 2324
MspI	CCGG	5 cut(s)	77, 152, 329, 684, 1920
MspR9I	CCNGG	6 cut(s)	77, 78, 209, 250, 1397, 1921
Mva1269I	GAATGC	1 cut(s)	271
MvaI	CCWGG	3 cut(s)	209, 250, 1397
MvnI	CGCG	3 cut(s)	263, 1806, 2445
NarI	GGCGCC	1 cut(s)	687
NciI	CCSGG	3 cut(s)	77, 78, 1921
NdeII	GATC	8 cut(s)	85, 93, 833, 1017, 1658, 1878, 2035, 2266
NheI	GCTAGC	1 cut(s)	1102
NmeAIII	GCCGAG	2 cut(s)	170, 1024
NmuCI	GTSAC	8 cut(s)	496, 745, 935, 1042, 1091, 1800, 2429, 2453
NsbI	TGCGCA	1 cut(s)	735
NspI	RCATGY	5 cut(s)	310, 565, 1228, 1559, 1801
OliI	CACNNNNGTG	3 cut(s)	881, 933, 2057
PaeR7I	CTCGAG	2 cut(s)	829, 2425
PagI	TCATGA	1 cut(s)	450
PasI	CCCWGGG	1 cut(s)	1396
PceI	AGGCCT	1 cut(s)	1203
PciI	ACATGT	3 cut(s)	561, 1224, 1797
PciSI	GCTCTTC	1 cut(s)	2232
PctI	GAATGC	1 cut(s)	271
PfeI	GAWTC	6 cut(s)	19, 173, 387, 485, 1145, 1602
PleI	GAGTC	7 cut(s)	149, 674, 1585, 2336, 2343, 2436, 2450
PluTI	GGCGCC	1 cut(s)	690
PmaCI	CACGTG	1 cut(s)	1309
PmlI	CACGTG	1 cut(s)	1309
PpsI	GAGTC	7 cut(s)	149, 674, 1585, 2336, 2343, 2436, 2450
Ppu21I	YACGTR	1 cut(s)	1309
PpuMI	RGGWCCY	3 cut(s)	211, 360, 825
PscI	ACATGT	3 cut(s)	561, 1224, 1797
PshBI	ATTAAT	1 cut(s)	984
Psp5II	RGGWCCY	3 cut(s)	211, 360, 825
Psp6I	CCWGG	3 cut(s)	207, 248, 1395
PspCI	CACGTG	1 cut(s)	1309
PspEI	GGTNACC	3 cut(s)	496, 745, 1091
PspGI	CCWGG	3 cut(s)	207, 248, 1395
PspPI	GGNCC	8 cut(s)	211, 360, 430, 825, 1393, 1932, 2024, 2083
PspPPI	RGGWCCY	3 cut(s)	211, 360, 825
PspXI	VCTCGAGB	1 cut(s)	2425
PstI	CTGCAG	1 cut(s)	1339
PstNI	CAGNNNCTG	1 cut(s)	2110
PsuI	RGATCY	3 cut(s)	85, 1658, 1878
PvuII	CAGCTG	2 cut(s)	1511, 2324
RseI	CAYNNNNRTG	6 cut(s)	881, 933, 1370, 1452, 1947, 2057
SapI	GCTCTTC	1 cut(s)	2232
SaqAI	TTAA	3 cut(s)	984, 1086, 1206
Sau3AI	GATC	8 cut(s)	85, 93, 833, 1017, 1658, 1878, 2035, 2266
Sau96I	GGNCC	8 cut(s)	211, 360, 430, 825, 1393, 1932, 2024, 2083
SchI	GAGTC	7 cut(s)	149, 674, 1586, 2336, 2343, 2437, 2450
ScrFI	CCNGG	6 cut(s)	77, 78, 209, 250, 1397, 1921
SduI	GDGCHC	3 cut(s)	83, 2013, 2227
SfaNI	GCATC	6 cut(s)	50, 118, 889, 1120, 1701, 1831
SfcI	CTRYAG	3 cut(s)	820, 1335, 2503
SfoI	GGCGCC	1 cut(s)	688
Sfr274I	CTCGAG	2 cut(s)	829, 2425
SinI	GGWCC	6 cut(s)	211, 360, 825, 1932, 2024, 2083
SlaI	CTCGAG	2 cut(s)	829, 2425
SmaI	CCCGGG	1 cut(s)	78
SmiMI	CAYNNNNRTG	6 cut(s)	881, 933, 1370, 1452, 1947, 2057
SmlI	CTYRAG	7 cut(s)	829, 1515, 1845, 2276, 2387, 2425, 2437
SmoI	CTYRAG	7 cut(s)	829, 1515, 1845, 2276, 2387, 2425, 2437
SseBI	AGGCCT	1 cut(s)	1203
SspDI	GGCGCC	1 cut(s)	686
SspI	AATATT	1 cut(s)	649
SspMI	CTAG	6 cut(s)	491, 1103, 1419, 1926, 2187, 2213
StuI	AGGCCT	1 cut(s)	1203
StyD4I	CCNGG	6 cut(s)	75, 76, 207, 248, 1395, 1919
StyI	CCWWGG	3 cut(s)	97, 762, 1992
TaaI	ACNGT	3 cut(s)	1091, 1857, 2056
TaiI	ACGT	1 cut(s)	1311
TaqII	GACCGA	1 cut(s)	1715
TatI	WGTACW	4 cut(s)	3, 1614, 1827, 2497
TauI	GCSGC	4 cut(s)	108, 761, 1073, 1809
TfiI	GAWTC	6 cut(s)	19, 173, 387, 485, 1145, 1602
Tru1I	TTAA	3 cut(s)	984, 1086, 1206
Tru9I	TTAA	3 cut(s)	984, 1086, 1206
TscAI	CASTG	2 cut(s)	1459, 2061
TseFI	GTSAC	8 cut(s)	496, 745, 935, 1042, 1091, 1800, 2429, 2453
Tsp45I	GTSAC	8 cut(s)	496, 745, 935, 1042, 1091, 1800, 2429, 2453
TspDTI	ATGAA	7 cut(s)	165, 257, 898, 1565, 1788, 1895, 2219
TspGWI	ACGGA	1 cut(s)	34
TspMI	CCCGGG	1 cut(s)	76
TspRI	CASTG	2 cut(s)	1459, 2061
VpaK11BI	GGWCC	6 cut(s)	211, 360, 825, 1932, 2024, 2083
VspI	ATTAAT	1 cut(s)	984
XagI	CCTNNNNNAGG	3 cut(s)	479, 2153, 2195
XapI	RAATTY	5 cut(s)	622, 905, 1567, 1691, 2309
XbaI	TCTAGA	1 cut(s)	490
XceI	RCATGY	5 cut(s)	310, 565, 1228, 1559, 1801
XhoI	CTCGAG	2 cut(s)	829, 2425
XmaI	CCCGGG	1 cut(s)	76
XmiI	GTMKAC	1 cut(s)	2130
XspI	CTAG	6 cut(s)	491, 1103, 1419, 1926, 2187, 2213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.