Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0004399

Physical Location & Seq

Genomic Coordinates Reverse (-)

2024786 .. 2028050

Gene Structure 3265 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G002300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2631 bp

ATGTCCACCGGAACCAACATTGTTTTCTTTCTCGCTTTTGTGTCTTCCTTCCTAAGTTTTGGCCTGCAAGCGGCGAGCCCGGACTCGTTGATGCTCGGCTGTGGCTCAGAAAATGAAGCCACAGATGCAGACGGGCGAAAATGGTTACCAGACTCGAAGTTTGTTACTGCTTCGAATTCCGTTCAGGCCCAAGCTCAGTACCAAGACCCTGCACTCAGTTCCGAGATCCCATACATGAAGGCCAGGATCTTCACCTCAAATGCCACGTACAAGTTGCCGGCCAAATCTAAGCAAAGGTACTTCCTCAGGCTACATTTCTATCCTGCAAACTATGGCAGCCACAATGCCGAGCAGGCCTTTTTTTCGGTTATGGCAAATGGAGTCACATTGTTGAAGAATTTCAGCGCCTCAATCACTTGCAAGGCGACCACGCAAGCTTATATCGTAAGAGAGTACTCCGTGGCTCCACTCACCAAGGATGATCTGGAACTCACATTTTCGGTCAACACAGGTTTTGCCTTTGTCAATGGCATTGAGTTTGTCCCCGCCCCAGATGACTTGTTCGACCAGGCTACGCTTGTTGGAAATCCGGATCAGTCCTTCGACAGCAAGAGCTCGAATTTGCAGACATTGTATAGGTTGAACGTCGGTGGGAGTTCCATTCCACCAACCAAGGACTCTGGCTCCTTAGGAAGGACTTGGTATGATGACAGTAGTTATATATTTGGTGCTAATACTGGGGTCCCCAATCTGGCTGACAATAAGACGAAAATTGAGTACAAAGATATCCCAGACTATTCTGCTCCTGTTGATATTTACACCACTGCAAGATCAATGGGTCCAAATGGGTCTGTAAATATAAATAGCAACCTCACATGGTTGTTCAACCAGGTTGATACCAAGTACATGTACATTGTGAGGCTACACTTTTGCGACAATAAAAACACCAAAGCTAATCAGGTTGTATTCACCATTTACATTAACAACCAAACCGCGGAGCCTGCAGCCGATGTGATCGGATGGTCAGGAGGGATCGGAAAAGCTGCATACAGAGACTACGCGACAATACTTGATGAAAAGAGTGGTGGCGACCTTTGGCTAACTTTGCATCCTAATCCGGATTCAAAACCACAATACTATGATACACTGCTCAACGGCGTGGAGATATTCAAGCTTGAAACTGGTAAAAGTTTGGCCGGTCCTAATCCCAAGCCTTCGGAAATGTTGGAAAAGAACGAGGAAGAGCAAAAGAAAAACTTCGAAAATCAAAAGGATAAGGAAACGGGTTCAAACAAAGCTCATGTGATTGGTGGAGCCGCAGGAGGAGCTGCAGCTGCTGGGATAGTGGCTGCATTGTGCATTGCTGTGTACCAAAGGAAGAAGCGAGTCCAAGGAACTGAATCACATACTAATAGCTGGCTTCCAATTTATGGCAACTCGCACACTAGTGGCACAAAATCCACAGGTTCGGGCAGGAGTAACGGTAGCAGACACCTATCGTCAGCTGCTCAAGGCCGGTGCAGGCGCTTCTCATTTGTTGAGATGAAACAGGCCACCAAGAATTTTGATGAGACTAATGTGATTGGTGTTGGAGGGTTTGGGAAGGTGTACAAGGGAGTTATTGACAATGGAGCTACCAAAGTGGCCATCAAGAGGTCTAACCCTCAATCCGAACAAGGAGTACATGAATTCCAGACCGAAATTGAGATGCTTTCGGAGTTGAGGCACAAGCATTTGGTTTCTCTTATTGGATTCTGTGAGGAAGACGATGAAATGTGCTTGGTTTATGACTACATGTCACGCGGCACACTTAGAGAGCACATTTACAAGGGCAACAAGACTCATTTGTCATGGAAGCAAAGGCTGGAGATTTGCATTGGAGCAGCTAAAGGACTTCACTACCTTCACACAGGGGCAAGATGGACAATCATTCATAGAGATGTCAAAACAACAAACATTCTCTTGGATGAGAATTGGGAGGCTAAGGTCTCTGATTTCGGGCTCTCGAAAACTGGCCCAGACTTGAACAAGGGTCACGTTAGCACAGTAGTGAAGGGTAGCTTTGGGTATTTGGATCCTGAATACTTCAAAAGACAACAACTGACTGAAAAATCTGATGTCTACTCCTTTGGGGTTGTCCTATTTGAGGTGTTGTGTGCAAGGCCTGCTCTAAATCCAAACCTTCCAAAAGAACAGGTTAGTCTAGCAGATTGGGCACTACATTGCCAAAAGAAAGGGATTCTAGAAGAAATCATTGACCCTCATTTGAAGGGCAAAGTGAACATGGAAAGCATCAATAAATTTGCAGACACAGCGGAGAAGTGCTTGTCTGAGTGCGGAGTTGATCGCCCGAGTTTGAATGACATCTTGTGGAACCTTGAGTTTGCTCTCCAGTTGCAGGAGGGGACAGATAGTGGGTCGACAGGATTGGCGTCACGAACCCGATCCGACTCTGATGATGCTAGTTTGAGAAACCAGCACATGATGGCCATACATTATAGCAACCTCAGTCTTGGAAGTGAGAGTGAAATAGGGGAAGAACAACCACCACCATCATCCAATAACAACGAAGCCACCCCCGATAATGATAGCGCAATCTTCTCGCAGATTGTCAATCCGACGGGTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016324 GO:0030427 GO:0035838 GO:0042995 GO:0044425 GO:0044459 GO:0044463 GO:0044464 GO:0045177 GO:0051286 GO:0071944 GO:0090404 GO:0090406 GO:0098590 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

876

Amino Acids

96.49

Weight (kDa)

6.0

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	32 - 167	2.2e-08	Malectin domain
Malectin_like	PF12819	33 - 391	2.4e-33	Malectin-like domain
Pkinase	PF00069	524 - 790	5.3e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	526 - 739	3.7e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004399)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04690 AT5G28680 AT5G28680
fragaria_vesca	FvH4_4g19690 FvH4_6g02670
malus_domestica	MD04G1226200.v1.1 MD12G1243300.v1.1 MD13G1154700.v1.1
prunus_persica	Prupe.1G117700_v2.0.a1 Prupe.6G345000_v2.0.a1
pyrus_communis	pycom04g19940 pycom13g13150
rosa_chinensis	RchiOBHm_Chr3g0450621 RchiOBHm_Chr4g0425141
rosa_laevigata	RLG00000007395 RLG00000025717
rosa_multiflora	Rmu_sc0000078.1_g000041 Rmu_ssc0000420.1_g000041
rosa_roxburghii	Rroxscaffold_5G00367230 Rroxscaffold_6G00428300
rosa_rugosa	Rorug02G0629100 Rorug04G0201800
rosa_samantha	Rh3AG029800 Rh3BG030200 Rh3CG029100 Rh3DG029900 Rh4AG258400 Rh4BG264200 Rh4CG275300 Rh4DG260300
rosa_wichuraiana	Rw3G002300 Rw4G022370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1846
AccB7I	CCANNNNNTGG	1 cut(s)	1430
AccI	GTMKAC	2 cut(s)	2121, 2420
AccII	CGCG	3 cut(s)	995, 1061, 1803
AccIII	TCCGGA	2 cut(s)	589, 1117
AciI	CCGC	8 cut(s)	71, 546, 993, 995, 1317, 1803, 2315, 2337
AclWI	GGATC	7 cut(s)	220, 254, 600, 1040, 2069, 2082, 2439
AcoI	YGGCCR	4 cut(s)	279, 1194, 1644, 2487
AcsI	RAATTY	6 cut(s)	175, 397, 619, 1561, 1688, 2300
AcyI	GRCGYC	1 cut(s)	2432
AfiI	CCNNNNNNNGG	8 cut(s)	70, 693, 751, 994, 1430, 1653, 2146, 2398
AflIII	ACRYGT	2 cut(s)	906, 1794
AhdI	GACNNNNNGTC	1 cut(s)	1795
AhlI	ACTAGT	1 cut(s)	1445
AjnI	CCWGG	3 cut(s)	242, 567, 888
AjuI	GAANNNNNNNTTGG	2 cut(s)	8, 40
AleI	CACNNNNGTG	2 cut(s)	1446, 2048
Alw21I	GWGCWC	2 cut(s)	617, 1821
Alw26I	GTCTC	3 cut(s)	1047, 1565, 1993
AlwI	GGATC	7 cut(s)	220, 254, 600, 1040, 2069, 2082, 2439
AlwNI	CAGNNNCTG	1 cut(s)	1337
Ama87I	CYCGRG	1 cut(s)	2350
Aor13HI	TCCGGA	2 cut(s)	589, 1117
ApeKI	GCWGC	9 cut(s)	336, 1004, 1043, 1328, 1331, 1334, 1349, 1505, 1883
ApoI	RAATTY	6 cut(s)	175, 397, 619, 1561, 1688, 2300
ArsI	GACNNNNNNTTYG	2 cut(s)	143, 175
Asp700I	GAANNNNTTC	2 cut(s)	398, 1256
AspLEI	GCGC	3 cut(s)	407, 1527, 2594
AspS9I	GGNCC	5 cut(s)	187, 742, 839, 1199, 2015
AsuC2I	CCSGG	1 cut(s)	80
AsuHPI	GGTGA	3 cut(s)	244, 463, 961
AsuII	TTCGAA	2 cut(s)	173, 1260
AvaI	CYCGRG	1 cut(s)	2350
AvaII	GGWCC	3 cut(s)	742, 839, 1199
AxyI	CCTNAGG	2 cut(s)	305, 688
BaeGI	GKGCMC	1 cut(s)	2218
BalI	TGGCCA	2 cut(s)	1646, 2489
BamHI	GGATCC	1 cut(s)	2074
BanII	GRGCYC	3 cut(s)	80, 617, 2004
BbsI	GAAGAC	2 cut(s)	36, 1770
Bbv12I	GWGCWC	2 cut(s)	617, 1821
BbvI	GCAGC	9 cut(s)	348, 1016, 1030, 1315, 1321, 1336, 1343, 1492, 1895
BccI	CCATC	5 cut(s)	1014, 1655, 1914, 2479, 2560
BceAI	ACGGC	1 cut(s)	1171
BcgI	CGANNNNNNTGC	2 cut(s)	2583, 2617
BciT130I	CCWGG	3 cut(s)	244, 569, 890
BcnI	CCSGG	1 cut(s)	80
BcoDI	GTCTC	3 cut(s)	1047, 1565, 1993
BcuI	ACTAGT	1 cut(s)	1445
BfaI	CTAG	4 cut(s)	1446, 2204, 2243, 2463
BfmI	CTRYAG	2 cut(s)	1002, 1329
BfoI	RGCGCY	2 cut(s)	408, 1528
BglI	GCCNNNNNGGC	1 cut(s)	353
BmcAI	AGTACT	1 cut(s)	455
Bme1390I	CCNGG	4 cut(s)	80, 244, 569, 890
Bme18I	GGWCC	3 cut(s)	742, 839, 1199
BmeRI	GACNNNNNGTC	1 cut(s)	1795
BmeT110I	CYCGRG	1 cut(s)	2350
BmgT120I	GGNCC	5 cut(s)	187, 742, 839, 1199, 2015
BmrFI	CCNGG	4 cut(s)	80, 244, 569, 890
BmrI	ACTGGG	1 cut(s)	747
BmsI	GCATC	6 cut(s)	81, 115, 1117, 1698, 2301, 2449
BmuI	ACTGGG	1 cut(s)	747
BpiI	GAAGAC	2 cut(s)	36, 1770
BpmI	CTGGAG	2 cut(s)	1886, 2375
Bpu10I	CCTNAGC	1 cut(s)	1983
Bpu14I	TTCGAA	2 cut(s)	173, 1260
BpuEI	CTTGAG	2 cut(s)	1494, 2399
BpuMI	CCSGG	1 cut(s)	80
BsaAI	YACGTR	1 cut(s)	267
BsaHI	GRCGYC	1 cut(s)	2432
BsaI	GGTCTC	1 cut(s)	1993
BsaJI	CCNNGG	5 cut(s)	459, 474, 672, 993, 1390
BsaWI	WCCGGW	3 cut(s)	8, 589, 1117
BsaXI	ACNNNNNCTCC	4 cut(s)	668, 698, 1468, 1498
Bsc4I	CCNNNNNNNGG	8 cut(s)	70, 693, 751, 994, 1430, 1653, 2146, 2398
Bse118I	RCCGGY	3 cut(s)	277, 1196, 1515
Bse1I	ACTGG	4 cut(s)	742, 1186, 2017, 2392
Bse21I	CCTNAGG	2 cut(s)	305, 688
Bse3DI	GCAATG	2 cut(s)	1359, 2221
BseAI	TCCGGA	2 cut(s)	589, 1117
BseBI	CCWGG	3 cut(s)	244, 569, 890
BseDI	CCNNGG	5 cut(s)	459, 474, 672, 993, 1390
BseGI	GGATG	5 cut(s)	484, 1025, 1108, 1972, 2555
BseLI	CCNNNNNNNGG	8 cut(s)	70, 693, 751, 994, 1430, 1653, 2146, 2398
BseMI	GCAATG	2 cut(s)	1359, 2221
BseMII	CTCAG	6 cut(s)	120, 209, 229, 319, 2322, 2521
BseNI	ACTGG	4 cut(s)	742, 1186, 2017, 2392
BseRI	GAGGAG	1 cut(s)	1338
BseSI	GKGCMC	1 cut(s)	2218
BseXI	GCAGC	9 cut(s)	348, 1016, 1030, 1315, 1321, 1336, 1343, 1492, 1895
BseYI	CCCAGC	1 cut(s)	1337
BsgI	GTGCAG	2 cut(s)	195, 1540
Bsh1236I	CGCG	3 cut(s)	995, 1061, 1803
BsiHKAI	GWGCWC	2 cut(s)	617, 1821
BsiHKCI	CYCGRG	1 cut(s)	2350
BsiSI	CCGG	7 cut(s)	9, 80, 278, 590, 1118, 1197, 1516
BslFI	GGGAC	3 cut(s)	527, 728, 2419
BslI	CCNNNNNNNGG	8 cut(s)	70, 693, 751, 994, 1430, 1653, 2146, 2398
BsmAI	GTCTC	3 cut(s)	1047, 1565, 1993
BsmFI	GGGAC	3 cut(s)	527, 728, 2419
Bso31I	GGTCTC	1 cut(s)	1993
BsoBI	CYCGRG	1 cut(s)	2350
Bsp119I	TTCGAA	2 cut(s)	173, 1260
Bsp1286I	GDGCHC	5 cut(s)	80, 617, 1821, 2004, 2218
Bsp13I	TCCGGA	2 cut(s)	589, 1117
Bsp1407I	TGTACA	2 cut(s)	909, 1608
BspACI	CCGC	8 cut(s)	71, 546, 993, 995, 1317, 1803, 2315, 2337
BspCNI	CTCAG	6 cut(s)	119, 208, 228, 318, 2323, 2520
BspEI	TCCGGA	2 cut(s)	589, 1117
BspFNI	CGCG	3 cut(s)	995, 1061, 1803
BspMAI	CTGCAG	2 cut(s)	1006, 1333
BspPI	GGATC	7 cut(s)	220, 254, 600, 1040, 2069, 2082, 2439
BspQI	GCTCTTC	1 cut(s)	1236
BspT104I	TTCGAA	2 cut(s)	173, 1260
BspTNI	GGTCTC	1 cut(s)	1993
BsrDI	GCAATG	2 cut(s)	1359, 2221
BsrFI	RCCGGY	3 cut(s)	277, 1196, 1515
BsrGI	TGTACA	2 cut(s)	909, 1608
BsrI	ACTGG	4 cut(s)	742, 1186, 2017, 2392
BssAI	RCCGGY	3 cut(s)	277, 1196, 1515
BssECI	CCNNGG	5 cut(s)	459, 474, 672, 993, 1390
BssNI	GRCGYC	1 cut(s)	2432
BssT1I	CCWWGG	3 cut(s)	474, 672, 1390
Bst2UI	CCWGG	3 cut(s)	244, 569, 890
Bst4CI	ACNGT	3 cut(s)	713, 1484, 2047
Bst6I	CTCTTC	1 cut(s)	1236
BstACI	GRCGYC	1 cut(s)	2432
BstAPI	GCANNNNNTGC	1 cut(s)	2165
BstAUI	TGTACA	2 cut(s)	909, 1608
BstBAI	YACGTR	1 cut(s)	267
BstBI	TTCGAA	2 cut(s)	173, 1260
BstDSI	CCRYGG	2 cut(s)	459, 993
BstEII	GGTNACC	1 cut(s)	144
BstENI	CCTNNNNNAGG	2 cut(s)	691, 2144
BstF5I	GGATG	5 cut(s)	484, 1025, 1108, 1972, 2555
BstFNI	CGCG	3 cut(s)	995, 1061, 1803
BstH2I	RGCGCY	2 cut(s)	408, 1528
BstHHI	GCGC	3 cut(s)	407, 1527, 2594
BstMAI	GTCTC	3 cut(s)	1047, 1565, 1993
BstMWI	GCNNNNNNNGC	9 cut(s)	125, 353, 1001, 1105, 1325, 1334, 2165, 2213, 2312
BstNI	CCWGG	3 cut(s)	244, 569, 890
BstNSI	RCATGY	2 cut(s)	910, 1798
BstPI	GGTNACC	1 cut(s)	144
BstSCI	CCNGG	4 cut(s)	78, 242, 567, 888
BstSFI	CTRYAG	2 cut(s)	1002, 1329
BstSLI	GKGCMC	1 cut(s)	2218
BstUI	CGCG	3 cut(s)	995, 1061, 1803
BstV1I	GCAGC	9 cut(s)	348, 1016, 1030, 1315, 1321, 1336, 1343, 1492, 1895
BstV2I	GAAGAC	2 cut(s)	36, 1770
BstX2I	RGATCY	3 cut(s)	225, 246, 2074
BstYI	RGATCY	3 cut(s)	225, 246, 2074
Bsu36I	CCTNAGG	2 cut(s)	305, 688
BtgI	CCRYGG	2 cut(s)	459, 993
BtsCI	GGATG	5 cut(s)	484, 1025, 1108, 1972, 2555
BtsI	GCAGTG	2 cut(s)	822, 1145
BtsIMutI	CAGTG	2 cut(s)	822, 1145
CaiI	CAGNNNCTG	1 cut(s)	1337
CfoI	GCGC	3 cut(s)	407, 1527, 2594
Cfr10I	RCCGGY	3 cut(s)	277, 1196, 1515
Cfr13I	GGNCC	5 cut(s)	187, 742, 839, 1199, 2015
Cfr42I	CCGCGG	1 cut(s)	996
CseI	GACGC	1 cut(s)	2421
CsiI	ACCWGGT	1 cut(s)	888
CviAII	CATG	9 cut(s)	235, 876, 907, 1301, 1685, 1795, 1851, 2284, 2482
DrdI	GACNNNNNNGTC	1 cut(s)	1846
DriI	GACNNNNNGTC	1 cut(s)	1795
DseDI	GACNNNNNNGTC	1 cut(s)	1846
EaeI	YGGCCR	4 cut(s)	279, 1194, 1644, 2487
Eam1104I	CTCTTC	1 cut(s)	1236
Eam1105I	GACNNNNNGTC	1 cut(s)	1795
EarI	CTCTTC	1 cut(s)	1236
Ecl136II	GAGCTC	1 cut(s)	615
Eco130I	CCWWGG	3 cut(s)	474, 672, 1390
Eco147I	AGGCCT	2 cut(s)	356, 2164
Eco24I	GRGCYC	3 cut(s)	80, 617, 2004
Eco31I	GGTCTC	1 cut(s)	1993
Eco32I	GATATC	1 cut(s)	787
Eco47I	GGWCC	3 cut(s)	742, 839, 1199
Eco53kI	GAGCTC	1 cut(s)	615
Eco81I	CCTNAGG	2 cut(s)	305, 688
Eco88I	CYCGRG	1 cut(s)	2350
Eco91I	GGTNACC	1 cut(s)	144
EcoICRI	GAGCTC	1 cut(s)	615
EcoNI	CCTNNNNNAGG	2 cut(s)	691, 2144
EcoO109I	RGGNCCY	1 cut(s)	742
EcoO65I	GGTNACC	1 cut(s)	144
EcoRI	GAATTC	2 cut(s)	175, 1688
EcoRII	CCWGG	3 cut(s)	242, 567, 888
EcoRV	GATATC	1 cut(s)	787
EcoT14I	CCWWGG	3 cut(s)	474, 672, 1390
EcoT38I	GRGCYC	3 cut(s)	80, 617, 2004
ErhI	CCWWGG	3 cut(s)	474, 672, 1390
FaeI	CATG	9 cut(s)	238, 879, 910, 1304, 1688, 1798, 1854, 2287, 2485
FalI	AAGNNNNNCTT	4 cut(s)	1241, 1273, 2045, 2077
FaqI	GGGAC	3 cut(s)	527, 728, 2419
FatI	CATG	9 cut(s)	234, 875, 906, 1300, 1684, 1794, 1850, 2283, 2481
FauI	CCCGC	1 cut(s)	553
FblI	GTMKAC	2 cut(s)	2121, 2420
FokI	GGATG	5 cut(s)	491, 1032, 1095, 1979, 2542
FriOI	GRGCYC	3 cut(s)	80, 617, 2004
FspBI	CTAG	4 cut(s)	1446, 2204, 2243, 2463
GlaI	GCGC	3 cut(s)	406, 1526, 2593
GsaI	CCCAGC	1 cut(s)	1341
GsuI	CTGGAG	2 cut(s)	1886, 2375
HaeII	RGCGCY	2 cut(s)	408, 1528
HapII	CCGG	7 cut(s)	9, 80, 278, 590, 1118, 1197, 1516
HgaI	GACGC	1 cut(s)	2421
HhaI	GCGC	3 cut(s)	407, 1527, 2594
Hin1I	GRCGYC	1 cut(s)	2432
Hin1II	CATG	9 cut(s)	238, 879, 910, 1304, 1688, 1798, 1854, 2287, 2485
Hin6I	GCGC	3 cut(s)	405, 1525, 2592
HinP1I	GCGC	3 cut(s)	405, 1525, 2592
HincII	GTYRAC	2 cut(s)	505, 2421
HindII	GTYRAC	2 cut(s)	505, 2421
HindIII	AAGCTT	2 cut(s)	435, 1172
HpaII	CCGG	7 cut(s)	9, 80, 278, 590, 1118, 1197, 1516
HphI	GGTGA	3 cut(s)	244, 463, 961
Hpy166II	GTNNAC	7 cut(s)	6, 505, 1369, 1609, 2122, 2281, 2421
Hpy8I	GTNNAC	7 cut(s)	6, 505, 1369, 1609, 2122, 2281, 2421
Hpy99I	CGWCG	2 cut(s)	650, 2623
HpyCH4III	ACNGT	3 cut(s)	713, 1484, 2047
HpyCH4IV	ACGT	3 cut(s)	266, 645, 2037
HpyF10VI	GCNNNNNNNGC	9 cut(s)	125, 353, 1001, 1105, 1325, 1334, 2165, 2213, 2312
HpySE526I	ACGT	3 cut(s)	266, 645, 2037
Hsp92I	GRCGYC	1 cut(s)	2432
Hsp92II	CATG	9 cut(s)	238, 879, 910, 1304, 1688, 1798, 1854, 2287, 2485
HspAI	GCGC	3 cut(s)	405, 1525, 2592
KflI	GGGWCCC	1 cut(s)	742
Kpn2I	TCCGGA	2 cut(s)	589, 1117
KroI	GCCGGC	1 cut(s)	277
KroNI	GCCGGC	1 cut(s)	279
KspI	CCGCGG	1 cut(s)	996
LguI	GCTCTTC	1 cut(s)	1236
LmnI	GCTCC	8 cut(s)	469, 689, 808, 997, 1313, 1325, 1631, 1880
Lsp1109I	GCAGC	9 cut(s)	348, 1016, 1030, 1315, 1321, 1336, 1343, 1492, 1895
LweI	GCATC	6 cut(s)	81, 115, 1117, 1698, 2301, 2449
MabI	ACCWGGT	1 cut(s)	888
MaeI	CTAG	4 cut(s)	1446, 2204, 2243, 2463
MaeII	ACGT	3 cut(s)	266, 645, 2037
MaeIII	GTNAC	7 cut(s)	144, 163, 382, 1478, 1797, 2033, 2433
MboII	GAAGA	9 cut(s)	36, 241, 406, 1253, 1390, 1775, 2258, 2549, 2590
MflI	RGATCY	3 cut(s)	225, 246, 2074
MhlI	GDGCHC	5 cut(s)	80, 617, 1821, 2004, 2218
MlsI	TGGCCA	2 cut(s)	1646, 2489
MluNI	TGGCCA	2 cut(s)	1646, 2489
MlyI	GAGTC	7 cut(s)	77, 146, 390, 671, 1395, 1834, 2444
MmeI	TCCRAC	4 cut(s)	562, 1206, 1570, 2472
Mox20I	TGGCCA	2 cut(s)	1646, 2489
MroI	TCCGGA	2 cut(s)	589, 1117
MroNI	GCCGGC	1 cut(s)	277
MroXI	GAANNNNTTC	2 cut(s)	398, 1256
MscI	TGGCCA	2 cut(s)	1646, 2489
MseI	TTAA	1 cut(s)	981
MslI	CAYNNNNRTG	4 cut(s)	1364, 1446, 1938, 2048
Msp20I	TGGCCA	2 cut(s)	1646, 2489
MspA1I	CMGCKG	4 cut(s)	995, 1334, 1505, 2315
MspI	CCGG	7 cut(s)	9, 80, 278, 590, 1118, 1197, 1516
MspR9I	CCNGG	4 cut(s)	80, 244, 569, 890
MvaI	CCWGG	3 cut(s)	244, 569, 890
MvnI	CGCG	3 cut(s)	995, 1061, 1803
MwoI	GCNNNNNNNGC	9 cut(s)	125, 353, 1001, 1105, 1325, 1334, 2165, 2213, 2312
NaeI	GCCGGC	1 cut(s)	279
NciI	CCSGG	1 cut(s)	80
NgoMIV	GCCGGC	1 cut(s)	277
NlaIII	CATG	9 cut(s)	238, 879, 910, 1304, 1688, 1798, 1854, 2287, 2485
NmeAIII	GCCGAG	2 cut(s)	75, 373
NmuCI	GTSAC	4 cut(s)	382, 1797, 2033, 2433
NspI	RCATGY	2 cut(s)	910, 1798
NspV	TTCGAA	2 cut(s)	173, 1260
OliI	CACNNNNGTG	2 cut(s)	1446, 2048
PceI	AGGCCT	2 cut(s)	356, 2164
PciI	ACATGT	2 cut(s)	906, 1794
PciSI	GCTCTTC	1 cut(s)	1236
PdiI	GCCGGC	1 cut(s)	279
PdmI	GAANNNNTTC	2 cut(s)	398, 1256
PfeI	GAWTC	4 cut(s)	1121, 1400, 1752, 2239
PflMI	CCANNNNNTGG	1 cut(s)	1430
PleI	GAGTC	7 cut(s)	77, 146, 389, 671, 1394, 1834, 2444
PpsI	GAGTC	7 cut(s)	77, 146, 389, 671, 1394, 1834, 2444
Ppu21I	YACGTR	1 cut(s)	267
PpuMI	RGGWCCY	1 cut(s)	742
PscI	ACATGT	2 cut(s)	906, 1794
Psp124BI	GAGCTC	1 cut(s)	617
Psp5II	RGGWCCY	1 cut(s)	742
Psp6I	CCWGG	3 cut(s)	242, 567, 888
PspEI	GGTNACC	1 cut(s)	144
PspFI	CCCAGC	1 cut(s)	1337
PspGI	CCWGG	3 cut(s)	242, 567, 888
PspPI	GGNCC	5 cut(s)	187, 742, 839, 1199, 2015
PspPPI	RGGWCCY	1 cut(s)	742
PsrI	GAACNNNNNNTAC	2 cut(s)	1665, 1697
PstI	CTGCAG	2 cut(s)	1006, 1333
PstNI	CAGNNNCTG	1 cut(s)	1337
PsuI	RGATCY	3 cut(s)	225, 246, 2074
PvuII	CAGCTG	2 cut(s)	1334, 1505
RseI	CAYNNNNRTG	4 cut(s)	1364, 1446, 1938, 2048
SacI	GAGCTC	1 cut(s)	617
SacII	CCGCGG	1 cut(s)	996
SalI	GTCGAC	1 cut(s)	2419
SapI	GCTCTTC	1 cut(s)	1236
SaqAI	TTAA	1 cut(s)	981
Sau96I	GGNCC	5 cut(s)	187, 742, 839, 1199, 2015
ScaI	AGTACT	1 cut(s)	455
SchI	GAGTC	7 cut(s)	77, 146, 390, 671, 1395, 1834, 2444
ScrFI	CCNGG	4 cut(s)	80, 244, 569, 890
SduI	GDGCHC	5 cut(s)	80, 617, 1821, 2004, 2218
SexAI	ACCWGGT	1 cut(s)	888
SfaNI	GCATC	6 cut(s)	81, 115, 1117, 1698, 2301, 2449
SfcI	CTRYAG	2 cut(s)	1002, 1329
Sfr303I	CCGCGG	1 cut(s)	996
SfuI	TTCGAA	2 cut(s)	173, 1260
SgrBI	CCGCGG	1 cut(s)	996
SinI	GGWCC	3 cut(s)	742, 839, 1199
SmiMI	CAYNNNNRTG	4 cut(s)	1364, 1446, 1938, 2048
SmlI	CTYRAG	2 cut(s)	1509, 2378
SmoI	CTYRAG	2 cut(s)	1509, 2378
SpeI	ACTAGT	1 cut(s)	1445
SseBI	AGGCCT	2 cut(s)	356, 2164
SsiI	CCGC	8 cut(s)	71, 546, 993, 995, 1317, 1803, 2315, 2337
SspMI	CTAG	4 cut(s)	1446, 2204, 2243, 2463
SstI	GAGCTC	1 cut(s)	617
StuI	AGGCCT	2 cut(s)	356, 2164
StyD4I	CCNGG	4 cut(s)	78, 242, 567, 888
StyI	CCWWGG	3 cut(s)	474, 672, 1390
TaaI	ACNGT	3 cut(s)	713, 1484, 2047
TaiI	ACGT	3 cut(s)	269, 648, 2040
TaqI	TCGA	9 cut(s)	155, 173, 564, 603, 617, 1260, 2006, 2420, 2626
TaqII	GACCGA	2 cut(s)	490, 1712
TatI	WGTACW	6 cut(s)	453, 777, 903, 909, 1608, 1681
TauI	GCSGC	3 cut(s)	74, 1319, 1806
TfiI	GAWTC	4 cut(s)	1121, 1400, 1752, 2239
Tru1I	TTAA	1 cut(s)	981
Tru9I	TTAA	1 cut(s)	981
TscAI	CASTG	2 cut(s)	829, 1152
TseFI	GTSAC	4 cut(s)	382, 1797, 2033, 2433
TseI	GCWGC	9 cut(s)	336, 1004, 1043, 1328, 1331, 1334, 1349, 1505, 1883
Tsp45I	GTSAC	4 cut(s)	382, 1797, 2033, 2433
TspDTI	ATGAA	7 cut(s)	129, 251, 1089, 1559, 1701, 1785, 1922
TspGWI	ACGGA	2 cut(s)	169, 448
TspRI	CASTG	2 cut(s)	829, 1152
Van91I	CCANNNNNTGG	1 cut(s)	1430
VpaK11BI	GGWCC	3 cut(s)	742, 839, 1199
XagI	CCTNNNNNAGG	2 cut(s)	691, 2144
XapI	RAATTY	6 cut(s)	175, 397, 619, 1561, 1688, 2300
XbaI	TCTAGA	1 cut(s)	2242
XceI	RCATGY	2 cut(s)	910, 1798
XcmI	CCANNNNNNNNNTGG	1 cut(s)	481
XmiI	GTMKAC	2 cut(s)	2121, 2420
XmnI	GAANNNNTTC	2 cut(s)	398, 1256
XspI	CTAG	4 cut(s)	1446, 2204, 2243, 2463
ZrmI	AGTACT	1 cut(s)	455

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.