Rh4DG260300

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004399
Physical Location & Seq
Reverse (-)
47770380 .. 47772965
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG260300.1

Sequence Viewer

Length: 1368 bp
ATGATCTCCAACACCCAGATCTTATTTTCTCTGATCTTGTTATCTCTATGCACCATTCAAGTTCTTAGCGCAGACTCAAAGTCTTCCAATCCTTACATACTTAATTGTGGTTCTTCAAACGGTGGCACCGATTCCGATGGCCGGAAATGGTCATCGGATTCCCAGTATATTTCCGGGAATTCCACGACGGCGAGGGCCCAATACCAAGACCCTTCTCTTCCTTCCGAAATCCCATACATGTCTGCAAGAATCTTCAGCTCTTCATCCTCATACAAATTCTCTGTTTCACCAAAGCAACGTCTTTGGCTCAGACTCCATTTCTACCCTTCTTCTTATAACAGCCTTGACAAATCCGACTCGTATTTTGATGTGACCGCAAATGGGTTTACTCTCCTCAACAATTTCAGCGCCTCCATCACAGCCGAAGCTCTCACGCAGGCCTACATTGTCAAAGAATTCTCACTCATTCCTGTCGAGTCCGGGAATCTCAACATCACTTTCACCCCCTCTTCGTCACATAAGAAATCATTTGGTTTTGTGAACGGGATTGAGGTGATACCTATGCCGGATATATTCCATCCAGCCAAATTGGTCGGATTCGATGAACAAACGATCGATGTACAAAGCTCGACTCTCCAGACCATGTTTCGGCTCAATGTGGGTGGGCAGTATGTTGGGCCAGCCAACGACTCCGCCGGGCTTAACCGGACATGGTATGATGATTCGCCTTACTTGTTTGGTGCAGCATTTGGGGTCACATCTGCGGCTCAGAAGAATGTCTCGATTAGATACTCTAATAATGTGTCAGAATCTATTGCACCTGTTGATGTTTACACTTTTGTGAAAGATAAAGCTGGTGATGACGAACTGTGGGTGGCATTGCACCCATCTGTGTCAATGAAGCCTGAGTTCTATGATGCAATACTTAATGGATTGGAGGTCTTTAAGATCAATGATACTAGTGGCAACTTGGCAGGCCCCAACCCTGTGCCGTCTCAGATGCTTCAAGATGCTGAGGCGGCCGCGGCTGCGGCGAATAATCCATCCCCACCGGCAGCGGGATCAAAGAATAAGGGTTTGGTAATTGGTGGAGTCACTGGTGGGGCTGCTGGTGTTGTTGTAGCTGCCGCGGTTGGCTTTGTTGTGTACCAAAGGAAGAAGAGATCACATGAGTCCGGGGTTGGGGCTTGGTTGCCGCTGTATGACGGGGAAAGAGTTGTAGCTGAAGTTAGAGCAAATGATGCTTATGCTATGCACAATGCCACTCTGGCCATTGAAGAAGAGAGCACTACTGCAAGTGTGCCTGTTGATGAGTTGACCAATAGTACTGTTGTTTTTTCACAGTTTGTGAATCAACAAGGAAGATGA

Protein Analysis

455

Amino Acids

48.88

Weight (kDa)

5.06

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 34 - 278 6.6e-17 Malectin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AccB1I GGYRCC 1 cut(s) 125
AccII CGCG 2 cut(s) 1025, 1130
AclWI GGATC 1 cut(s) 1069
AcoI YGGCCR 3 cut(s) 139, 1020, 1269
AcsI RAATTY 3 cut(s) 178, 275, 455
AcuI CTGAAG 2 cut(s) 238, 1245
AfaI GTAC 3 cut(s) 621, 1148, 1327
AfiI CCNNNNNNNGG 5 cut(s) 141, 381, 648, 1058, 1182
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 4 cut(s) 59, 117, 1007, 1277
AhdI GACNNNNNGTC 1 cut(s) 79
AhlI ACTAGT 1 cut(s) 959
AjuI GAANNNNNNNTTGG 2 cut(s) 1061, 1093
AleI CACNNNNGTG 1 cut(s) 839
AluBI AGCT 6 cut(s) 258, 428, 627, 854, 1124, 1223
AluI AGCT 6 cut(s) 258, 428, 627, 854, 1124, 1223
Alw21I GWGCWC 1 cut(s) 1289
Alw26I GTCTC 2 cut(s) 784, 999
AlwI GGATC 1 cut(s) 1069
AoxI GGCC 7 cut(s) 139, 195, 438, 677, 976, 1020, 1269
ApaI GGGCCC 1 cut(s) 199
ApeKI GCWGC 5 cut(s) 743, 1028, 1055, 1106, 1124
ApoI RAATTY 3 cut(s) 178, 275, 455
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspLEI GCGC 2 cut(s) 71, 410
AspS9I GGNCC 4 cut(s) 195, 196, 677, 977
AsuC2I CCSGG 4 cut(s) 175, 481, 697, 1177
AsuHPI GGTGA 4 cut(s) 279, 493, 565, 869
BaeGI GKGCMC 1 cut(s) 199
BalI TGGCCA 1 cut(s) 1271
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 1289
BbvCI CCTCAGC 1 cut(s) 1014
BbvI GCAGC 5 cut(s) 755, 1015, 1067, 1093, 1111
BccI CCATC 5 cut(s) 131, 422, 585, 895, 1051
BceAI ACGGC 2 cut(s) 204, 976
BcnI CCSGG 4 cut(s) 175, 481, 697, 1177
BcoDI GTCTC 2 cut(s) 784, 999
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 1 cut(s) 960
BfoI RGCGCY 1 cut(s) 411
BglI GCCNNNNNGGC 1 cut(s) 1268
BglII AGATCT 1 cut(s) 18
BmcAI AGTACT 1 cut(s) 1327
Bme1390I CCNGG 4 cut(s) 175, 481, 697, 1177
BmeRI GACNNNNNGTC 1 cut(s) 79
BmgT120I GGNCC 4 cut(s) 195, 196, 677, 977
BmiI GGNNCC 3 cut(s) 127, 197, 979
BmrFI CCNGG 4 cut(s) 175, 481, 697, 1177
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 4 cut(s) 907, 990, 1000, 1231
BmuI ACTGGG 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 75
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 1014
BpuMI CCSGG 4 cut(s) 175, 481, 697, 1177
Bsa29I ATCGAT 1 cut(s) 615
BsaJI CCNNGG 3 cut(s) 1023, 1128, 1176
BsaWI WCCGGW 1 cut(s) 705
Bsc4I CCNNNNNNNGG 5 cut(s) 141, 381, 648, 1058, 1182
Bse118I RCCGGY 1 cut(s) 1051
Bse1I ACTGG 2 cut(s) 163, 1102
Bse3DI GCAATG 1 cut(s) 878
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 3 cut(s) 1023, 1128, 1176
BseGI GGATG 3 cut(s) 263, 577, 1043
BseLI CCNNNNNNNGG 5 cut(s) 141, 381, 648, 1058, 1182
BseMI GCAATG 1 cut(s) 878
BseMII CTCAG 5 cut(s) 322, 782, 897, 1005, 1010
BseNI ACTGG 2 cut(s) 163, 1102
BseRI GAGGAG 1 cut(s) 383
BseSI GKGCMC 1 cut(s) 199
BseX3I CGGCCG 1 cut(s) 1020
BseXI GCAGC 5 cut(s) 755, 1015, 1067, 1093, 1111
BsgI GTGCAG 1 cut(s) 762
Bsh1236I CGCG 2 cut(s) 1025, 1130
Bsh1285I CGRYCG 2 cut(s) 615, 1023
BshFI GGCC 7 cut(s) 141, 197, 440, 679, 978, 1022, 1271
BshNI GGYRCC 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 615
BsiEI CGRYCG 2 cut(s) 615, 1023
BsiHKAI GWGCWC 1 cut(s) 1289
BsiSI CCGG 8 cut(s) 142, 174, 480, 566, 696, 706, 1052, 1176
BslI CCNNNNNNNGG 5 cut(s) 141, 381, 648, 1058, 1182
BsmAI GTCTC 2 cut(s) 784, 999
BsmBI CGTCTC 1 cut(s) 999
BsnI GGCC 7 cut(s) 141, 197, 440, 679, 978, 1022, 1271
Bsp120I GGGCCC 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 199, 1289
Bsp1407I TGTACA 1 cut(s) 619
Bsp143I GATC 7 cut(s) 3, 18, 33, 612, 948, 1061, 1163
BspANI GGCC 7 cut(s) 141, 197, 440, 679, 978, 1022, 1271
BspCNI CTCAG 5 cut(s) 321, 781, 898, 1006, 1009
BspDI ATCGAT 1 cut(s) 615
BspFNI CGCG 2 cut(s) 1025, 1130
BspLI GGNNCC 3 cut(s) 127, 197, 979
BspPI GGATC 1 cut(s) 1069
BspQI GCTCTTC 1 cut(s) 265
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 878
BsrFI RCCGGY 1 cut(s) 1051
BsrGI TGTACA 1 cut(s) 619
BsrI ACTGG 2 cut(s) 163, 1102
BssAI RCCGGY 1 cut(s) 1051
BssECI CCNNGG 3 cut(s) 1023, 1128, 1176
BssMI GATC 7 cut(s) 3, 18, 33, 612, 948, 1061, 1163
Bst4CI ACNGT 4 cut(s) 122, 870, 1330, 1344
Bst6I CTCTTC 5 cut(s) 222, 265, 514, 1154, 1275
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstAUI TGTACA 1 cut(s) 619
BstC8I GCNNGC 3 cut(s) 438, 681, 976
BstDEI CTNAG 6 cut(s) 65, 308, 768, 906, 996, 1014
BstDSI CCRYGG 2 cut(s) 1023, 1128
BstF5I GGATG 3 cut(s) 263, 577, 1043
BstFNI CGCG 2 cut(s) 1025, 1130
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 2 cut(s) 71, 410
BstKTI GATC 7 cut(s) 6, 21, 36, 615, 951, 1064, 1166
BstMAI GTCTC 2 cut(s) 784, 999
BstMBI GATC 7 cut(s) 3, 18, 33, 612, 948, 1061, 1163
BstMCI CGRYCG 2 cut(s) 615, 1023
BstMWI GCNNNNNNNGC 6 cut(s) 1019, 1025, 1028, 1031, 1241, 1268
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 4 cut(s) 173, 479, 695, 1175
BstSLI GKGCMC 1 cut(s) 199
BstUI CGCG 2 cut(s) 1025, 1130
BstV1I GCAGC 5 cut(s) 755, 1015, 1067, 1093, 1111
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BstZI CGGCCG 1 cut(s) 1020
Bsu15I ATCGAT 1 cut(s) 615
BsuRI GGCC 7 cut(s) 141, 197, 440, 679, 978, 1022, 1271
BsuTUI ATCGAT 1 cut(s) 615
BtgI CCRYGG 2 cut(s) 1023, 1128
BtsCI GGATG 3 cut(s) 263, 577, 1043
BtsIMutI CAGTG 1 cut(s) 1095
Cac8I GCNNGC 3 cut(s) 438, 681, 976
CciNI GCGGCCGC 1 cut(s) 1020
CfoI GCGC 2 cut(s) 71, 410
Cfr10I RCCGGY 1 cut(s) 1051
Cfr13I GGNCC 4 cut(s) 195, 196, 677, 977
Cfr42I CCGCGG 2 cut(s) 1026, 1131
ClaI ATCGAT 1 cut(s) 615
Csp6I GTAC 3 cut(s) 620, 1147, 1326
CspCI CAANNNNNGTGG 2 cut(s) 643, 678
CviAII CATG 4 cut(s) 238, 643, 711, 1169
CviQI GTAC 3 cut(s) 620, 1147, 1326
DdeI CTNAG 6 cut(s) 65, 308, 768, 906, 996, 1014
DpnI GATC 7 cut(s) 5, 20, 35, 614, 950, 1063, 1165
DpnII GATC 7 cut(s) 3, 18, 33, 612, 948, 1061, 1163
DriI GACNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 3 cut(s) 139, 1020, 1269
EagI CGGCCG 1 cut(s) 1020
Eam1104I CTCTTC 5 cut(s) 222, 265, 514, 1154, 1275
Eam1105I GACNNNNNGTC 1 cut(s) 79
EarI CTCTTC 5 cut(s) 222, 265, 514, 1154, 1275
EciI GGCGGA 1 cut(s) 682
EclXI CGGCCG 1 cut(s) 1020
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 199
Eco52I CGGCCG 1 cut(s) 1020
Eco57I CTGAAG 2 cut(s) 238, 1245
EcoO109I RGGNCCY 2 cut(s) 195, 977
EcoRI GAATTC 2 cut(s) 178, 455
EcoT38I GRGCYC 1 cut(s) 199
Esp3I CGTCTC 1 cut(s) 999
FaeI CATG 4 cut(s) 241, 646, 714, 1172
FatI CATG 4 cut(s) 237, 642, 710, 1168
FauI CCCGC 1 cut(s) 1051
FokI GGATG 3 cut(s) 250, 564, 1030
FriOI GRGCYC 1 cut(s) 199
FspBI CTAG 1 cut(s) 960
GlaI GCGC 2 cut(s) 70, 409
GsuI CTGGAG 1 cut(s) 620
HaeII RGCGCY 1 cut(s) 411
HaeIII GGCC 7 cut(s) 141, 197, 440, 679, 978, 1022, 1271
HapII CCGG 8 cut(s) 142, 174, 480, 566, 696, 706, 1052, 1176
HhaI GCGC 2 cut(s) 71, 410
Hin1II CATG 4 cut(s) 241, 646, 714, 1172
Hin6I GCGC 2 cut(s) 69, 408
HinP1I GCGC 2 cut(s) 69, 408
HincII GTYRAC 1 cut(s) 1317
HindII GTYRAC 1 cut(s) 1317
HpaII CCGG 8 cut(s) 142, 174, 480, 566, 696, 706, 1052, 1176
HphI GGTGA 4 cut(s) 279, 493, 565, 869
Hpy166II GTNNAC 5 cut(s) 387, 541, 832, 1147, 1317
Hpy188III TCNNGA 3 cut(s) 637, 781, 1007
Hpy8I GTNNAC 5 cut(s) 387, 541, 832, 1147, 1317
Hpy99I CGWCG 1 cut(s) 190
HpyAV CCTTC 3 cut(s) 222, 231, 336
HpyCH4III ACNGT 4 cut(s) 122, 870, 1330, 1344
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 8 cut(s) 51, 245, 743, 818, 883, 920, 1255, 1295
HpyF10VI GCNNNNNNNGC 6 cut(s) 1019, 1025, 1028, 1031, 1241, 1268
HpyF3I CTNAG 6 cut(s) 65, 308, 768, 906, 996, 1014
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 4 cut(s) 241, 646, 714, 1172
HspAI GCGC 2 cut(s) 69, 408
KspI CCGCGG 2 cut(s) 1026, 1131
Kzo9I GATC 7 cut(s) 3, 18, 33, 612, 948, 1061, 1163
LguI GCTCTTC 1 cut(s) 265
Lsp1109I GCAGC 5 cut(s) 755, 1015, 1067, 1093, 1111
LweI GCATC 4 cut(s) 907, 990, 1000, 1231
MaeI CTAG 1 cut(s) 960
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 4 cut(s) 370, 513, 754, 1093
MalI GATC 7 cut(s) 5, 20, 35, 614, 950, 1063, 1165
MboI GATC 7 cut(s) 3, 18, 33, 612, 948, 1061, 1163
MflI RGATCY 1 cut(s) 18
MhlI GDGCHC 2 cut(s) 199, 1289
MlsI TGGCCA 1 cut(s) 1271
MluCI AATT 7 cut(s) 103, 178, 275, 400, 455, 587, 1083
MluNI TGGCCA 1 cut(s) 1271
MlyI GAGTC 8 cut(s) 68, 306, 350, 485, 625, 683, 1101, 1181
MmeI TCCRAC 3 cut(s) 33, 378, 574
MnlI CCTC 8 cut(s) 186, 277, 404, 421, 517, 544, 931, 1009
Mox20I TGGCCA 1 cut(s) 1271
MscI TGGCCA 1 cut(s) 1271
MseI TTAA 4 cut(s) 102, 702, 927, 945
MslI CAYNNNNRTG 1 cut(s) 839
Msp20I TGGCCA 1 cut(s) 1271
MspA1I CMGCKG 4 cut(s) 1025, 1058, 1130, 1198
MspI CCGG 8 cut(s) 142, 174, 480, 566, 696, 706, 1052, 1176
MspR9I CCNGG 4 cut(s) 175, 481, 697, 1177
MvnI CGCG 2 cut(s) 1025, 1130
MwoI GCNNNNNNNGC 6 cut(s) 1019, 1025, 1028, 1031, 1241, 1268
NciI CCSGG 4 cut(s) 175, 481, 697, 1177
NdeII GATC 7 cut(s) 3, 18, 33, 612, 948, 1061, 1163
NlaIII CATG 4 cut(s) 241, 646, 714, 1172
NlaIV GGNNCC 3 cut(s) 127, 197, 979
NmuCI GTSAC 4 cut(s) 370, 513, 754, 1093
NotI GCGGCCGC 1 cut(s) 1020
NspI RCATGY 1 cut(s) 241
OliI CACNNNNGTG 1 cut(s) 839
PceI AGGCCT 1 cut(s) 440
PciI ACATGT 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 265
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 8 cut(s) 131, 158, 249, 484, 597, 722, 809, 1351
PfoI TCCNGGA 2 cut(s) 173, 479
Ple19I CGATCG 1 cut(s) 615
PleI GAGTC 8 cut(s) 68, 306, 350, 484, 625, 683, 1100, 1180
PpsI GAGTC 8 cut(s) 68, 306, 350, 484, 625, 683, 1100, 1180
PscI ACATGT 1 cut(s) 237
PsiI TTATAA 1 cut(s) 336
PspN4I GGNNCC 3 cut(s) 127, 197, 979
PspOMI GGGCCC 1 cut(s) 195
PspPI GGNCC 4 cut(s) 195, 196, 677, 977
PsuI RGATCY 1 cut(s) 18
PvuI CGATCG 1 cut(s) 615
RsaI GTAC 3 cut(s) 621, 1148, 1327
RsaNI GTAC 3 cut(s) 620, 1147, 1326
RseI CAYNNNNRTG 1 cut(s) 839
SacII CCGCGG 2 cut(s) 1026, 1131
SapI GCTCTTC 1 cut(s) 265
SaqAI TTAA 4 cut(s) 102, 702, 927, 945
Sau3AI GATC 7 cut(s) 3, 18, 33, 612, 948, 1061, 1163
Sau96I GGNCC 4 cut(s) 195, 196, 677, 977
ScaI AGTACT 1 cut(s) 1327
SchI GAGTC 8 cut(s) 68, 306, 350, 485, 625, 683, 1101, 1181
ScrFI CCNGG 4 cut(s) 175, 481, 697, 1177
SduI GDGCHC 2 cut(s) 199, 1289
SfaNI GCATC 4 cut(s) 907, 990, 1000, 1231
Sfr303I CCGCGG 2 cut(s) 1026, 1131
SgrBI CCGCGG 2 cut(s) 1026, 1131
SmiMI CAYNNNNRTG 1 cut(s) 839
SpeI ACTAGT 1 cut(s) 959
Sse9I AATT 7 cut(s) 103, 178, 275, 400, 455, 587, 1083
SseBI AGGCCT 1 cut(s) 440
SspMI CTAG 1 cut(s) 960
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 4 cut(s) 173, 479, 695, 1175
TaaI ACNGT 4 cut(s) 122, 870, 1330, 1344
TaiI ACGT 1 cut(s) 301
TaqI TCGA 5 cut(s) 474, 600, 615, 629, 782
TasI AATT 7 cut(s) 103, 178, 275, 400, 455, 587, 1083
TatI WGTACW 2 cut(s) 619, 1325
TauI GCSGC 7 cut(s) 767, 1022, 1025, 1028, 1034, 1130, 1198
TfiI GAWTC 8 cut(s) 131, 158, 249, 484, 597, 722, 809, 1351
Tru1I TTAA 4 cut(s) 102, 702, 927, 945
Tru9I TTAA 4 cut(s) 102, 702, 927, 945
TscAI CASTG 1 cut(s) 1102
TseFI GTSAC 4 cut(s) 370, 513, 754, 1093
TseI GCWGC 5 cut(s) 743, 1028, 1055, 1106, 1124
Tsp45I GTSAC 4 cut(s) 370, 513, 754, 1093
TspDTI ATGAA 3 cut(s) 252, 618, 914
TspRI CASTG 1 cut(s) 1102
XapI RAATTY 3 cut(s) 178, 275, 455
XceI RCATGY 1 cut(s) 241
XspI CTAG 1 cut(s) 960
ZrmI AGTACT 1 cut(s) 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.