MD13G1154700.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004399
Physical Location & Seq
Forward (+)
12062098 .. 12064680
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1154700.v1.1.491

Sequence Viewer

Length: 2583 bp
ATGATCTCCAAAACCCACATTTTACTTTCTCTGATCATATTATCCTTCTCCTTAAACGCCATTCATGTTCTCAGCAAAGACACTGGCTCTGAATCTTACATCATTGCTTGCGGCTCTTCGAGCGGTGGCACCGACTCCGACGGCCGCAGATGGGTACCAGATTCCCGGTTTCTAAAATCCTCGGAGAATTCAAAGACCGCGACGGCGCAATACCAAGATCCTTCTCTGCCTTCCCAAATCCCGTACATGTCTGCAAGAATTTTCAACTCTGCAGCATCCTACAAGTTCTTTGTTTCCCCACGACAGCGCCTACTGGTCAGGCTCCATTTTTATCCGTCTTCCTATGACGGCCTCGAGGCTTCGAACTCGTATTTCGACGTGATCGCAAACGGGTTTTCACTCCTCCGCAATTTCAGCGCCGCCATTACAACCCAAGCTCTCACGCAGGCCTACATCATGAGAGAATTCTCACTCGTTCCCGCCCAATCCGGCAATCTCAACATAACATTCGTACCCTCGTCGCAACATGATAAGTCCTATGCTTTTGTCAATGGCATTGAGGTAATTTCGATGCCGGCCGAGATGTTTCAGCCAGCGAACATGATCGGGTTTCAGGACCAAACCATCGATGTACAAAACTCTTCCCTTCAGACAATGTTTAGGCTCAATGTCGGTGGACAGTTTATTCCGACAAGAAATGATTCGGGGCTGACAAGAACATGGTACGACGATTCGCCGTACTTGTTCGGTGCAGCATTTGGAGTCACATGTCAGGCTGAGAAAAATGTTAGCATAAAATATCCTAGTAATTTGCCGGAGTACATTGCTCCTCCCAACGTCTACGGCACTGCGCGATCGATGGGACCGAATTCGAAAATCAATCAGAATTACAATCTCACATGGGTTTTCACCGTTGATGCAAATTTCACATATGTTGTTAGGTTACATTTCTGTGAGCTTCAACTCACCAGGATCAATCAGAGGGTGTTCGATATTTTTCTCAACAACCAAACAGCGCAGCAGACTGCGGACGTTATTGCTTGGTCAGGGTCCATAGGAGTGCCGGTTTACAAGGATTATGCAACTTTTGTTAATGACAGAGATGGTGACGAAGAAATATGGGTGGCATTGCATCCATCTGTGTCCGAGAAACCAGAGTACTATGATTCGATTCTTAATGGTTTGGAGATCTTTAAGCTCAATGATACGCGTGGGAATTTAGCCGGTCCCAATCCTGTGCCATCTAAGATGCTTCAAGACGCTGAGGCTGCTGCAGCAAGTACTTTTTCTCCCCCAGCAGGGTCCAAGAGCAAGGGTGAAGTAATTGGAATAGCTGGCGGGGCTGCTGGCGGCGCGGCTGTGGTTGCGGCATTGTGCATTGCTGTGTACATAAAGAAGAAGAGAAAGAGTGGAATGGATCCCGGGATGGGCAATTGGTTGCCTCTTTATGGTAACTCTAACACATCAACTATTTCGGGCAAGAGTAATACAGGTAGCAGCCATCTTTCGAGCCTGGCTGCAGGTCTTTGCAGGCATTTCTCATTGCCTGAGATCAAACATGGCACCAAGAATTTCGACGAATCTCAAGTTATTGGGGTTGGAGGATTTGGGAAGGTTTACAAGGGCATTATTGATGGAGGCACCAAAGTGGCTATTAAAAGATCAAATCCATCTTCAGAGCAAGGTGTTCACGAGTTCCAAACGGAAATCGAGATGCTTTCAAAGCTCAGGCACAGGCATTTAGTCTCTTTGATTGGTTTTTGCGAAGAGGACGGCGAGATGATTCTGGTTTATGATTACATGGCTAATGGGACTCTAAGGGAGCATTTGTACAAGAGCAAAAATCCTTCCTTGTCATGGAAGCAAAGGTTGGAAATTTGCATTGGAGCTGCTAGGGGACTGCATTATCTTCACACTGGTGCAAGATACACCATCATCCACAGAGATGTGAAAACCACAAACATATTGTTGGATGAGAATTGGGTAGCTAAAGTCTCTGACTTTGGGCTATCAAAAACAGGTCCTAATCTTCACCAAACCCATGTGAGTACAATGGTGAAGGGCAGCTTTGGGTACTTGGACCCCGAATATTTTCGGAGGCAACAATTGACGGAAAAATCTGATGTTTACTCTTTTGGGGTAGTCTTGTTTGAGGTCTTGTGTGCAAGGCCGGCCCTCAATGCTTCATTGCCTAAGGAGCAAGTTAGTCTTGCAGATTGGGCAATACATTGTCTAAAAAAGGGAATTCTCTTCGAAATTATTGATCCACATCTCAAGTCAGGGCCGCCTATTAATCCTGAGTGCTTGAAAAAGTTTGCAGAGACAGCTGAGAAATGCTTAGCTGATCATGGACTTGAACGCCCTTCAATGGGGGACGTGCTTTGGAACCTTGAGTTTGCTCTTCAACTGCATGAAAACCCTGATGGAGAAGCAGTTGCTGCTCAAGATAAAGCAAATGATGCTTATGCTATGCACAATGCCACACTGACAATTGAGGAAGAGACCGTTTCAAGTGAGGCTACGATCGATGACTTAAACACAAGTGCAGTTTTTTCACAAATAGTGAATCCAAGAGGAAGATGA

Protein Analysis

861

Amino Acids

94.63

Weight (kDa)

6.24

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 35 - 399 1.3e-37 Malectin-like domain
Malectin PF11721 266 - 363 2.8e-10 Malectin domain
Pkinase PF00069 526 - 794 3.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 528 - 793 4.7e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 1511
Acc65I GGTACC 1 cut(s) 154
AccB1I GGYRCC 4 cut(s) 128, 154, 1562, 1640
AccBSI CCGCTC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 840
AccII CGCG 4 cut(s) 200, 853, 1210, 1355
AclWI GGATC 5 cut(s) 212, 980, 1412, 1425, 2258
AcoI YGGCCR 2 cut(s) 142, 576
AcsI RAATTY 9 cut(s) 187, 258, 464, 868, 922, 1216, 1570, 1875, 2244
AcuI CTGAAG 2 cut(s) 632, 1659
AfiI CCNNNNNNNGG 8 cut(s) 151, 1298, 1299, 1427, 1448, 1857, 2368, 2369
AflIII ACRYGT 3 cut(s) 246, 767, 1208
AjiI CACGTC 2 cut(s) 379, 2377
AjnI CCWGG 2 cut(s) 968, 1512
AjuI GAANNNNNNNTTGG 4 cut(s) 1853, 1866, 1885, 1898
AleI CACNNNNGTG 2 cut(s) 1646, 1917
Alw26I GTCTC 4 cut(s) 1750, 1999, 2315, 2495
AlwI GGATC 5 cut(s) 212, 980, 1412, 1425, 2258
AlwNI CAGNNNCTG 1 cut(s) 2438
Ama87I CYCGRG 2 cut(s) 353, 1421
AoxI GGCC 7 cut(s) 142, 349, 447, 576, 2168, 2172, 2282
ApoI RAATTY 9 cut(s) 187, 258, 464, 868, 922, 1216, 1570, 1875, 2244
AseI ATTAAT 1 cut(s) 2292
Asp700I GAANNNNTTC 2 cut(s) 700, 2091
Asp718I GGTACC 1 cut(s) 154
AspLEI GCGC 6 cut(s) 208, 309, 419, 853, 1018, 1355
AspS9I GGNCC 9 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080, 2173, 2282
AsuC2I CCSGG 3 cut(s) 166, 1422, 1423
AsuHPI GGTGA 6 cut(s) 901, 958, 1118, 1328, 2024, 2068
AsuII TTCGAA 3 cut(s) 362, 872, 2253
AvaI CYCGRG 2 cut(s) 353, 1421
AvaII GGWCC 7 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080
AxyI CCTNAGG 1 cut(s) 2193
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BamHI GGATCC 1 cut(s) 1417
BanI GGYRCC 4 cut(s) 128, 154, 1562, 1640
BauI CACGAG 1 cut(s) 1691
BbsI GAAGAC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 1263
BceAI ACGGC 6 cut(s) 157, 219, 364, 721, 859, 1789
BciT130I CCWGG 2 cut(s) 970, 1514
BclI TGATCA 2 cut(s) 33, 2344
BcnI CCSGG 3 cut(s) 166, 1422, 1423
BcoDI GTCTC 4 cut(s) 1750, 1999, 2315, 2495
BfaI CTAG 2 cut(s) 804, 1893
BfmI CTRYAG 3 cut(s) 270, 1272, 1518
BfoI RGCGCY 2 cut(s) 310, 420
BfuAI ACCTGC 1 cut(s) 1511
BglII AGATCT 1 cut(s) 1188
BlpI GCTNAGC 1 cut(s) 2338
BmcAI AGTACT 2 cut(s) 1160, 1282
Bme1390I CCNGG 5 cut(s) 166, 970, 1422, 1423, 1514
Bme18I GGWCC 7 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080
BmeT110I CYCGRG 2 cut(s) 353, 1421
BmgBI CACGTC 2 cut(s) 379, 2377
BmgT120I GGNCC 9 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080, 2173, 2282
BmrFI CCNGG 5 cut(s) 166, 970, 1422, 1423, 1514
BmsI GCATC 7 cut(s) 284, 561, 907, 1141, 1239, 1704, 2449
BpiI GAAGAC 1 cut(s) 330
Bpu10I CCTNAGC 2 cut(s) 1263, 1727
Bpu1102I GCTNAGC 1 cut(s) 2338
Bpu14I TTCGAA 3 cut(s) 362, 872, 2253
BpuEI CTTGAG 4 cut(s) 1569, 2258, 2411, 2427
BpuMI CCSGG 3 cut(s) 166, 1422, 1423
Bsa29I ATCGAT 3 cut(s) 627, 857, 2526
BsaBI GATNNNNATC 1 cut(s) 2268
BsaI GGTCTC 1 cut(s) 2495
BsaJI CCNNGG 2 cut(s) 180, 1421
BsaXI ACNNNNNCTCC 8 cut(s) 1050, 1080, 1273, 1303, 1814, 1844, 2418, 2448
Bsc4I CCNNNNNNNGG 8 cut(s) 151, 1298, 1299, 1427, 1448, 1857, 2368, 2369
Bse118I RCCGGY 4 cut(s) 574, 1063, 1223, 2170
Bse1I ACTGG 3 cut(s) 88, 318, 1921
Bse21I CCTNAGG 1 cut(s) 2193
Bse3DI GCAATG 6 cut(s) 102, 822, 1127, 1377, 1541, 2186
Bse8I GATNNNNATC 1 cut(s) 2268
BseBI CCWGG 2 cut(s) 970, 1514
BseCI ATCGAT 3 cut(s) 627, 857, 2526
BseDI CCNNGG 2 cut(s) 180, 1421
BseGI GGATG 5 cut(s) 275, 1132, 1431, 1935, 1978
BseJI GATNNNNATC 1 cut(s) 2268
BseLI CCNNNNNNNGG 8 cut(s) 151, 1298, 1299, 1427, 1448, 1857, 2368, 2369
BseMI GCAATG 6 cut(s) 102, 822, 1127, 1377, 1541, 2186
BseMII CTCAG 7 cut(s) 85, 768, 1254, 1539, 1741, 2289, 2319
BseNI ACTGG 3 cut(s) 88, 318, 1921
BseRI GAGGAG 2 cut(s) 392, 819
BseX3I CGGCCG 2 cut(s) 142, 576
BseYI CCCAGC 1 cut(s) 1294
BsgI GTGCAG 2 cut(s) 771, 2565
Bsh1236I CGCG 4 cut(s) 200, 853, 1210, 1355
Bsh1285I CGRYCG 4 cut(s) 145, 579, 857, 2526
BshFI GGCC 7 cut(s) 144, 351, 449, 578, 2170, 2174, 2284
BshNI GGYRCC 4 cut(s) 128, 154, 1562, 1640
BshVI ATCGAT 3 cut(s) 627, 857, 2526
BsiEI CGRYCG 4 cut(s) 145, 579, 857, 2526
BsiHKCI CYCGRG 2 cut(s) 353, 1421
BsiSI CCGG 8 cut(s) 166, 489, 575, 815, 1064, 1224, 1422, 2171
BslFI GGGAC 5 cut(s) 876, 1212, 1825, 1911, 2387
BslI CCNNNNNNNGG 8 cut(s) 151, 1298, 1299, 1427, 1448, 1857, 2368, 2369
BsmAI GTCTC 4 cut(s) 1750, 1999, 2315, 2495
BsmFI GGGAC 5 cut(s) 876, 1212, 1825, 1911, 2387
BsnI GGCC 7 cut(s) 144, 351, 449, 578, 2170, 2174, 2284
Bso31I GGTCTC 1 cut(s) 2495
BsoBI CYCGRG 2 cut(s) 353, 1421
Bsp119I TTCGAA 3 cut(s) 362, 872, 2253
Bsp1407I TGTACA 3 cut(s) 631, 1386, 1830
Bsp1720I GCTNAGC 1 cut(s) 2338
BspANI GGCC 7 cut(s) 144, 351, 449, 578, 2170, 2174, 2284
BspCNI CTCAG 7 cut(s) 84, 769, 1255, 1540, 1740, 2290, 2320
BspDI ATCGAT 3 cut(s) 627, 857, 2526
BspFNI CGCG 4 cut(s) 200, 853, 1210, 1355
BspHI TCATGA 1 cut(s) 456
BspMAI CTGCAG 3 cut(s) 274, 1276, 1522
BspMI ACCTGC 1 cut(s) 1511
BspPI GGATC 5 cut(s) 212, 980, 1412, 1425, 2258
BspQI GCTCTTC 2 cut(s) 121, 2406
BspT104I TTCGAA 3 cut(s) 362, 872, 2253
BspT107I GGYRCC 4 cut(s) 128, 154, 1562, 1640
BspTNI GGTCTC 1 cut(s) 2495
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 6 cut(s) 102, 822, 1127, 1377, 1541, 2186
BsrFI RCCGGY 4 cut(s) 574, 1063, 1223, 2170
BsrGI TGTACA 3 cut(s) 631, 1386, 1830
BsrI ACTGG 3 cut(s) 88, 318, 1921
BssAI RCCGGY 4 cut(s) 574, 1063, 1223, 2170
BssECI CCNNGG 2 cut(s) 180, 1421
BssSI CACGAG 1 cut(s) 1691
Bst2BI CACGAG 1 cut(s) 1691
Bst2UI CCWGG 2 cut(s) 970, 1514
Bst4CI ACNGT 3 cut(s) 681, 913, 2506
Bst6I CTCTTC 7 cut(s) 121, 646, 1394, 1761, 2255, 2406, 2493
BstAPI GCANNNNNTGC 2 cut(s) 2438, 2459
BstAUI TGTACA 3 cut(s) 631, 1386, 1830
BstBI TTCGAA 3 cut(s) 362, 872, 2253
BstC8I GCNNGC 8 cut(s) 109, 447, 576, 594, 1336, 1348, 1532, 2172
BstF5I GGATG 5 cut(s) 275, 1132, 1431, 1935, 1978
BstFNI CGCG 4 cut(s) 200, 853, 1210, 1355
BstH2I RGCGCY 2 cut(s) 310, 420
BstHHI GCGC 6 cut(s) 208, 309, 419, 853, 1018, 1355
BstMAI GTCTC 4 cut(s) 1750, 1999, 2315, 2495
BstMCI CGRYCG 4 cut(s) 145, 579, 857, 2526
BstNI CCWGG 2 cut(s) 970, 1514
BstNSI RCATGY 2 cut(s) 250, 771
BstSCI CCNGG 5 cut(s) 164, 968, 1420, 1421, 1512
BstSFI CTRYAG 3 cut(s) 270, 1272, 1518
BstUI CGCG 4 cut(s) 200, 853, 1210, 1355
BstV2I GAAGAC 1 cut(s) 330
BstX2I RGATCY 3 cut(s) 217, 1188, 1417
BstYI RGATCY 3 cut(s) 217, 1188, 1417
BstZI CGGCCG 2 cut(s) 142, 576
Bsu15I ATCGAT 3 cut(s) 627, 857, 2526
Bsu36I CCTNAGG 1 cut(s) 2193
BsuRI GGCC 7 cut(s) 144, 351, 449, 578, 2170, 2174, 2284
BsuTUI ATCGAT 3 cut(s) 627, 857, 2526
BtrI CACGTC 2 cut(s) 379, 2377
BtsCI GGATG 5 cut(s) 275, 1132, 1431, 1935, 1978
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 4 cut(s) 81, 846, 1914, 2483
BveI ACCTGC 1 cut(s) 1511
Cac8I GCNNGC 8 cut(s) 109, 447, 576, 594, 1336, 1348, 1532, 2172
CaiI CAGNNNCTG 1 cut(s) 2438
CciI TCATGA 1 cut(s) 456
CfoI GCGC 6 cut(s) 208, 309, 419, 853, 1018, 1355
Cfr10I RCCGGY 4 cut(s) 574, 1063, 1223, 2170
Cfr13I GGNCC 9 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080, 2173, 2282
Cfr9I CCCGGG 1 cut(s) 1421
ClaI ATCGAT 3 cut(s) 627, 857, 2526
CseI GACGC 1 cut(s) 1268
CspCI CAANNNNNGTGG 2 cut(s) 655, 690
EaeI YGGCCR 2 cut(s) 142, 576
EagI CGGCCG 2 cut(s) 142, 576
Eam1104I CTCTTC 7 cut(s) 121, 646, 1394, 1761, 2255, 2406, 2493
EarI CTCTTC 7 cut(s) 121, 646, 1394, 1761, 2255, 2406, 2493
EclXI CGGCCG 2 cut(s) 142, 576
Eco147I AGGCCT 1 cut(s) 449
Eco31I GGTCTC 1 cut(s) 2495
Eco47I GGWCC 7 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080
Eco52I CGGCCG 2 cut(s) 142, 576
Eco57I CTGAAG 2 cut(s) 632, 1659
Eco81I CCTNAGG 1 cut(s) 2193
Eco88I CYCGRG 2 cut(s) 353, 1421
EcoO109I RGGNCCY 1 cut(s) 2021
EcoRI GAATTC 4 cut(s) 187, 464, 868, 2244
EcoRII CCWGG 2 cut(s) 968, 1512
FalI AAGNNNNNCTT 4 cut(s) 2051, 2083, 2193, 2225
FaqI GGGAC 5 cut(s) 876, 1212, 1825, 1911, 2387
FauI CCCGC 2 cut(s) 487, 1331
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 2 cut(s) 33, 2344
FblI GTMKAC 1 cut(s) 840
FokI GGATG 5 cut(s) 262, 1119, 1438, 1922, 1985
FseI GGCCGGCC 1 cut(s) 2174
FspBI CTAG 2 cut(s) 804, 1893
GlaI GCGC 6 cut(s) 207, 308, 418, 852, 1017, 1354
GsaI CCCAGC 1 cut(s) 1298
HaeII RGCGCY 2 cut(s) 310, 420
HaeIII GGCC 7 cut(s) 144, 351, 449, 578, 2170, 2174, 2284
HapII CCGG 8 cut(s) 166, 489, 575, 815, 1064, 1224, 1422, 2171
HgaI GACGC 1 cut(s) 1268
HhaI GCGC 6 cut(s) 208, 309, 419, 853, 1018, 1355
Hin6I GCGC 6 cut(s) 206, 307, 417, 851, 1016, 1353
HinP1I GCGC 6 cut(s) 206, 307, 417, 851, 1016, 1353
HpaII CCGG 8 cut(s) 166, 489, 575, 815, 1064, 1224, 1422, 2171
HphI GGTGA 6 cut(s) 901, 958, 1118, 1328, 2024, 2068
Hpy166II GTNNAC 7 cut(s) 677, 841, 1069, 1387, 1618, 1690, 2128
Hpy188III TCNNGA 7 cut(s) 457, 614, 1256, 1691, 1711, 2297, 2444
Hpy8I GTNNAC 7 cut(s) 677, 841, 1069, 1387, 1618, 1690, 2128
Hpy99I CGWCG 6 cut(s) 143, 205, 380, 523, 731, 1580
HpyAV CCTTC 8 cut(s) 55, 231, 240, 656, 1606, 1857, 2053, 2373
HpyCH4III ACNGT 3 cut(s) 681, 913, 2506
HpyCH4IV ACGT 4 cut(s) 378, 837, 1032, 2376
HpySE526I ACGT 4 cut(s) 378, 837, 1032, 2376
HspAI GCGC 6 cut(s) 206, 307, 417, 851, 1016, 1353
KpnI GGTACC 1 cut(s) 158
KroI GCCGGC 2 cut(s) 574, 2170
KroNI GCCGGC 2 cut(s) 576, 2172
Ksp22I TGATCA 2 cut(s) 33, 2344
LguI GCTCTTC 2 cut(s) 121, 2406
LmnI GCTCC 5 cut(s) 327, 832, 1822, 1886, 2197
LweI GCATC 7 cut(s) 284, 561, 907, 1141, 1239, 1704, 2449
MaeI CTAG 2 cut(s) 804, 1893
MaeII ACGT 4 cut(s) 378, 837, 1032, 2376
MaeIII GTNAC 4 cut(s) 763, 942, 1106, 1451
MbiI CCGCTC 1 cut(s) 123
MfeI CAATTG 3 cut(s) 1432, 2105, 2490
MflI RGATCY 3 cut(s) 217, 1188, 1417
MluI ACGCGT 1 cut(s) 1208
MlyI GAGTC 3 cut(s) 128, 771, 1807
MmeI TCCRAC 5 cut(s) 162, 713, 1579, 1851, 1950
MroNI GCCGGC 2 cut(s) 574, 2170
MroXI GAANNNNTTC 2 cut(s) 700, 2091
MseI TTAA 7 cut(s) 53, 1092, 1176, 1194, 1656, 2292, 2534
MslI CAYNNNNRTG 6 cut(s) 951, 1058, 1382, 1646, 1917, 1944
MspA1I CMGCKG 1 cut(s) 2327
MspI CCGG 8 cut(s) 166, 489, 575, 815, 1064, 1224, 1422, 2171
MspR9I CCNGG 5 cut(s) 166, 970, 1422, 1423, 1514
MunI CAATTG 3 cut(s) 1432, 2105, 2490
MvaI CCWGG 2 cut(s) 970, 1514
MvnI CGCG 4 cut(s) 200, 853, 1210, 1355
NaeI GCCGGC 2 cut(s) 576, 2172
NciI CCSGG 3 cut(s) 166, 1422, 1423
NdeI CATATG 1 cut(s) 931
NgoMIV GCCGGC 2 cut(s) 574, 2170
NmeAIII GCCGAG 1 cut(s) 604
NmuCI GTSAC 2 cut(s) 763, 1106
NspI RCATGY 2 cut(s) 250, 771
NspV TTCGAA 3 cut(s) 362, 872, 2253
OliI CACNNNNGTG 2 cut(s) 1646, 1917
PaeR7I CTCGAG 1 cut(s) 353
PagI TCATGA 1 cut(s) 456
PceI AGGCCT 1 cut(s) 449
PciI ACATGT 2 cut(s) 246, 767
PciSI GCTCTTC 2 cut(s) 121, 2406
PcsI WCGNNNNNNNCGW 1 cut(s) 863
PdiI GCCGGC 2 cut(s) 576, 2172
PdmI GAANNNNTTC 2 cut(s) 700, 2091
PfeI GAWTC 9 cut(s) 92, 161, 701, 731, 1166, 1171, 1580, 1783, 2566
Ple19I CGATCG 2 cut(s) 857, 2526
PleI GAGTC 3 cut(s) 128, 770, 1807
PpsI GAGTC 3 cut(s) 128, 770, 1807
PpuMI RGGWCCY 1 cut(s) 2021
PscI ACATGT 2 cut(s) 246, 767
PshBI ATTAAT 1 cut(s) 2292
Psp5II RGGWCCY 1 cut(s) 2021
Psp6I CCWGG 2 cut(s) 968, 1512
PspFI CCCAGC 1 cut(s) 1294
PspGI CCWGG 2 cut(s) 968, 1512
PspPI GGNCC 9 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080, 2173, 2282
PspPPI RGGWCCY 1 cut(s) 2021
PspXI VCTCGAGB 1 cut(s) 353
PstI CTGCAG 3 cut(s) 274, 1276, 1522
PstNI CAGNNNCTG 1 cut(s) 2438
PsuI RGATCY 3 cut(s) 217, 1188, 1417
PvuI CGATCG 2 cut(s) 857, 2526
PvuII CAGCTG 1 cut(s) 2327
RigI GGCCGGCC 1 cut(s) 2174
RseI CAYNNNNRTG 6 cut(s) 951, 1058, 1382, 1646, 1917, 1944
SapI GCTCTTC 2 cut(s) 121, 2406
SaqAI TTAA 7 cut(s) 53, 1092, 1176, 1194, 1656, 2292, 2534
Sau96I GGNCC 9 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080, 2173, 2282
ScaI AGTACT 2 cut(s) 1160, 1282
SchI GAGTC 3 cut(s) 128, 771, 1807
ScrFI CCNGG 5 cut(s) 166, 970, 1422, 1423, 1514
SfaNI GCATC 7 cut(s) 284, 561, 907, 1141, 1239, 1704, 2449
SfcI CTRYAG 3 cut(s) 270, 1272, 1518
Sfr274I CTCGAG 1 cut(s) 353
SfuI TTCGAA 3 cut(s) 362, 872, 2253
SinI GGWCC 7 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080
SlaI CTCGAG 1 cut(s) 353
SmaI CCCGGG 1 cut(s) 1423
SmiMI CAYNNNNRTG 6 cut(s) 951, 1058, 1382, 1646, 1917, 1944
SmlI CTYRAG 5 cut(s) 353, 1584, 2273, 2390, 2442
SmoI CTYRAG 5 cut(s) 353, 1584, 2273, 2390, 2442
SseBI AGGCCT 1 cut(s) 449
SspI AATATT 1 cut(s) 2090
SspMI CTAG 2 cut(s) 804, 1893
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 5 cut(s) 164, 968, 1420, 1421, 1512
TaaI ACNGT 3 cut(s) 681, 913, 2506
TaiI ACGT 4 cut(s) 381, 840, 1035, 2379
TaqII GACCGA 1 cut(s) 880
TatI WGTACW 7 cut(s) 631, 819, 1158, 1280, 1386, 1830, 2048
TauI GCSGC 7 cut(s) 114, 147, 422, 1353, 1358, 1370, 2287
TfiI GAWTC 9 cut(s) 92, 161, 701, 731, 1166, 1171, 1580, 1783, 2566
Tru1I TTAA 7 cut(s) 53, 1092, 1176, 1194, 1656, 2292, 2534
Tru9I TTAA 7 cut(s) 53, 1092, 1176, 1194, 1656, 2292, 2534
TscAI CASTG 4 cut(s) 88, 853, 1921, 2490
TseFI GTSAC 2 cut(s) 763, 1106
Tsp45I GTSAC 2 cut(s) 763, 1106
TspDTI ATGAA 3 cut(s) 53, 2175, 2427
TspGWI ACGGA 3 cut(s) 324, 1718, 2126
TspMI CCCGGG 1 cut(s) 1421
TspRI CASTG 4 cut(s) 88, 853, 1921, 2490
VpaK11BI GGWCC 7 cut(s) 616, 863, 1050, 1226, 1302, 2021, 2080
VspI ATTAAT 1 cut(s) 2292
XapI RAATTY 9 cut(s) 187, 258, 464, 868, 922, 1216, 1570, 1875, 2244
XceI RCATGY 2 cut(s) 250, 771
XhoI CTCGAG 1 cut(s) 353
XmaI CCCGGG 1 cut(s) 1421
XmiI GTMKAC 1 cut(s) 840
XmnI GAANNNNTTC 2 cut(s) 700, 2091
XspI CTAG 2 cut(s) 804, 1893
ZrmI AGTACT 2 cut(s) 1160, 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.