Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0004399

Physical Location & Seq

Genomic Coordinates Reverse (-)

47732633 .. 47735495

Gene Structure 2863 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G022370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2409 bp

ATGATCTCCAACACCCAGATCTTATTTTCTCTGATCTTGTTATCTCTATGCACCATTCAAGTTCTTAGCGCAGACTCAAAGTCTTCCAATCCTTACATACTTAATTGTGGTTCTTCAAACGGTGGCACCGATTCCGATGGCCGGAAATGGTCATCGGATTCCCAGTATATTTCCGGGAATTCCACGACGGCGAGGGCCCAATACCAAGACCCTTCTCTTCCTTCCGAAATCCCATACATGTCTGCAAGAATCTTCAGCTCTTCATCCTCATACAAATTCTCTGTTTCACCAAAGCAACGTCTTTGGCTCAGACTCCATTTCTACCCTTCTTCCTATAACAGCCTTGACAAATCCGACTCGTATTTTGATGTGACCGCAAATGGGTTTACTCTCCTCAACAATTTCAGCGCCTCCATCACAGCCGAAGCTCTCACGCAGGCCTACATTGTCAAAGAATTCTCACTCATTCCTGTCGAGTCCGGGAATCTCAACATCACTTTCACCCCCTCTTCGTCACATAAGAAATCATTTGCTTTTGTGAACGGGATTGAGGTGATACCTATGCCGGATATATTCCATCCAGCCAAATTGGTCGGATTCGATGAACAAACGATCGATGTACAAAGCTCGACTCTCCAGACCATGTTTCGGCTCAATGTGGGTGGGCAGTATGTTGGGCCAGCCAACGACTCCGCCGGGCTTAACCGGACATGGTATGATGATTCGCCTTACTTGTTTGGTGCAGCATTTGGGGTCACATCTGAGGCTCAGAAGAATGTCTCGATTAGATACTCTAATAATGTGTCAGAATCTATTGCACCTGTTGATGTTTACAGTACTGCTAGATCAATGGGACCTGACCCGGAGGTCAACTCGAATTACAATCTCACATGGGTTTTTCAGGTGGATGCTAATTTCACTTATGTTGTGAGGTTACATTTCTGTGAGCTTCAGGTCACCAAGGTCAATCAGAGAGTGTTTGATATATATATTGGGGTTCCTCTTTATAAAGATTACGCAGCTTTTGTGAAAGATAAAGCTGGTGATGACGAACTGTGGGTGGCATTGCACCCATCTGTGTCAATGAAGCCTGAGTTCTATGATGCAATACTTAATGGATTGGAGGTCTTTAAGATCAATGATACTAGTGGCAACTTGGCAGGCCCCAACCCTAATAAGGGTTTGGTAATTGGTGGAGTCACTGGTGGGGCTGCTGGTGTTGTTGTAGCTGCCGCGGTTGGCTTTGTTGTGTACCAAAGGAAGAAGAGATCACATGAGTCCGGGGTTGGGGCTTGGTTGCCGCTGTATGGTACGTCTACCACATTGACTTCTCGAAAGAGTGGTACTAATACCGGTAGTACTAGCCATGTTTCGAGTTTGGCTGCTGGTCTATGTAGGCATTTCTCATTATCCGAGATCAAACATGGGTTTGGGAAGGTTTACAAAGGGTTGATTGATGGAGCGTCAACGGAAGTGGCTATAAAGAGATCCAATCCATCTTCCGAACAAGGAGTTAATGAGTTCCAAACTGAAATCGAGATGCTTTCGAGGCTTAGGCACAGGCATTTGGTCTCCTTGATTGGTTTTTGTGAAGAAGAAGGCGAGATGATTTTGGTTTATGATTACATGGCTAATGGGACTCTTAGGGAGCACCTCTACAAGTGCAACAAACCTCCATTGTCATGGAAGCAAAGGTTGGAAATTTGTATAGGAGCTGCGAGGGGACTGCATTATCTTCATACAGGTGCAAGGTACACTATCATCCATAGAGATGTGAAAACAACAAACATTCTCTTGGATGAGAATTGGGTAGCTAAAGTCTCTGATTTCGGGCTATCCAAAACGGGGCCTAATCTTCACCAAACCCATGTTAGTACAATGGTGAAGGGTAGCTTCGGGTACTTGGACCCTGAATACTTCCGCAGGCAACAATTGACTGAAAAATCAGATGTTTACTCATTTGGGGTAGTTCTATTTGAAGTTTTGTGTGCAAGGCCAGCACTCGATGCTACATTGCCTAAGGAGCAAGTTAGTCTTGCAGATTGGGCTATGCACTGTCAAAAACGAGGAACTCTTCACGAAATCATCGATCCACATCTTAAGGGGACATCAATAAATCCCGAATGTCTTAAAAAATTCGCAGAGACAGCTGAGAAGTGTTTATCCGATCATGGACTATATCGTCCTTCAATGGGTGATGTCCTTTGGAACCTTGAGTTTGCTCTTCAATTGCTTGAACATCCAGACGGGGAAAGAGTTGTAGCTGAAGTTAGAGCAAATGATGCTTATGCTATGCACAATGCCACTCTGGCCATTGAAGAAGAGAGCACTACTGCAAGTGTGCCTGTTGATGAGTTGACCAATAGTACTGTTGTTTTTTCACAGTTTGTGAATCAACAAGGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016324 GO:0030427 GO:0035838 GO:0042995 GO:0044425 GO:0044459 GO:0044463 GO:0044464 GO:0045177 GO:0051286 GO:0071944 GO:0090404 GO:0090406 GO:0098590 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

802

Amino Acids

88.56

Weight (kDa)

6.04

Isoelectric Point (pI)

39.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	34 - 378	5.4e-31	Malectin-like domain
Malectin	PF11721	237 - 333	6.4e-09	Malectin domain
Pkinase	PF00069	476 - 731	1.7e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	477 - 731	1.9e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004399)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04690 AT5G28680 AT5G28680
fragaria_vesca	FvH4_4g19690 FvH4_6g02670
malus_domestica	MD04G1226200.v1.1 MD12G1243300.v1.1 MD13G1154700.v1.1
prunus_persica	Prupe.1G117700_v2.0.a1 Prupe.6G345000_v2.0.a1
pyrus_communis	pycom04g19940 pycom13g13150
rosa_chinensis	RchiOBHm_Chr3g0450621 RchiOBHm_Chr4g0425141
rosa_laevigata	RLG00000007395 RLG00000025717
rosa_multiflora	Rmu_sc0000078.1_g000041 Rmu_ssc0000420.1_g000041
rosa_roxburghii	Rroxscaffold_5G00367230 Rroxscaffold_6G00428300
rosa_rugosa	Rorug02G0629100 Rorug04G0201800
rosa_samantha	Rh3AG029800 Rh3BG030200 Rh3CG029100 Rh3DG029900 Rh4AG258400 Rh4BG264200 Rh4CG275300 Rh4DG260300
rosa_wichuraiana	Rw3G002300 Rw4G022370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1008
AasI	GACNNNNNNGTC	2 cut(s)	866, 2181
AccB1I	GGYRCC	1 cut(s)	125
AccI	GTMKAC	1 cut(s)	1316
AccII	CGCG	1 cut(s)	1235
AciI	CCGC	6 cut(s)	375, 693, 1233, 1235, 1301, 1921
AclWI	GGATC	2 cut(s)	1482, 2084
AcoI	YGGCCR	2 cut(s)	139, 2310
AcsI	RAATTY	5 cut(s)	178, 275, 455, 1701, 2135
AcuI	CTGAAG	3 cut(s)	238, 935, 2286
AfiI	CCNNNNNNNGG	9 cut(s)	141, 381, 648, 1177, 1178, 1287, 1307, 1845, 2192
AflII	CTTAAG	1 cut(s)	2099
AflIII	ACRYGT	1 cut(s)	237
AgeI	ACCGGT	1 cut(s)	1352
AgsI	TTSAA	7 cut(s)	59, 117, 1979, 2190, 2228, 2237, 2318
AhdI	GACNNNNNGTC	1 cut(s)	79
AhlI	ACTAGT	1 cut(s)	1145
AjuI	GAANNNNNNNTTGG	2 cut(s)	1679, 1711
Alw21I	GWGCWC	2 cut(s)	1653, 2330
Alw26I	GTCTC	4 cut(s)	784, 1576, 1825, 2138
AlwI	GGATC	2 cut(s)	1482, 2084
AoxI	GGCC	8 cut(s)	139, 195, 438, 677, 1162, 1847, 1994, 2310
ApaI	GGGCCC	1 cut(s)	199
ApeKI	GCWGC	6 cut(s)	743, 1019, 1211, 1229, 1382, 1715
ApoI	RAATTY	5 cut(s)	178, 275, 455, 1701, 2135
ArsI	GACNNNNNNTTYG	2 cut(s)	347, 379
AsiGI	ACCGGT	1 cut(s)	1352
AspLEI	GCGC	2 cut(s)	71, 410
AspS9I	GGNCC	7 cut(s)	195, 196, 677, 854, 1163, 1847, 1906
AsuC2I	CCSGG	5 cut(s)	175, 481, 697, 863, 1282
AsuHPI	GGTGA	8 cut(s)	279, 493, 565, 949, 1055, 1850, 1894, 2207
AvaII	GGWCC	2 cut(s)	854, 1906
AxyI	CCTNAGG	1 cut(s)	2019
BaeGI	GKGCMC	1 cut(s)	199
BalI	TGGCCA	1 cut(s)	2312
BanI	GGYRCC	1 cut(s)	125
BanII	GRGCYC	1 cut(s)	199
BbsI	GAAGAC	1 cut(s)	75
Bbv12I	GWGCWC	2 cut(s)	1653, 2330
BbvI	GCAGC	6 cut(s)	755, 1031, 1198, 1216, 1369, 1702
BccI	CCATC	6 cut(s)	131, 422, 585, 1081, 1451, 1504
BceAI	ACGGC	1 cut(s)	204
BcgI	CGANNNNNNTGC	2 cut(s)	1708, 1742
BcnI	CCSGG	5 cut(s)	175, 481, 697, 863, 1282
BcoDI	GTCTC	4 cut(s)	784, 1576, 1825, 2138
BcuI	ACTAGT	1 cut(s)	1145
BfaI	CTAG	3 cut(s)	843, 1146, 1362
BfoI	RGCGCY	1 cut(s)	411
BfrI	CTTAAG	1 cut(s)	2099
BglI	GCCNNNNNGGC	1 cut(s)	2309
BglII	AGATCT	1 cut(s)	18
BisI	GCNGC	8 cut(s)	744, 1020, 1212, 1230, 1233, 1301, 1383, 1716
BlsI	GCNGC	8 cut(s)	745, 1021, 1213, 1231, 1234, 1302, 1384, 1717
BmcAI	AGTACT	3 cut(s)	838, 1360, 2368
Bme1390I	CCNGG	5 cut(s)	175, 481, 697, 863, 1282
Bme18I	GGWCC	2 cut(s)	854, 1906
BmeRI	GACNNNNNGTC	1 cut(s)	79
BmgT120I	GGNCC	7 cut(s)	195, 196, 677, 854, 1163, 1847, 1906
BmiI	GGNNCC	8 cut(s)	127, 197, 855, 999, 1165, 1848, 1908, 2210
BmrFI	CCNGG	5 cut(s)	175, 481, 697, 863, 1282
BmrI	ACTGGG	1 cut(s)	157
BmsI	GCATC	5 cut(s)	898, 1093, 1530, 1996, 2272
BmuI	ACTGGG	1 cut(s)	157
BpiI	GAAGAC	1 cut(s)	75
BplI	GAGNNNNNCTC	2 cut(s)	857, 889
BpmI	CTGGAG	1 cut(s)	620
Bpu10I	CCTNAGC	1 cut(s)	1553
BpuEI	CTTGAG	1 cut(s)	2234
BpuMI	CCSGG	5 cut(s)	175, 481, 697, 863, 1282
Bsa29I	ATCGAT	2 cut(s)	615, 2088
BsaBI	GATNNNNATC	1 cut(s)	2094
BsaI	GGTCTC	1 cut(s)	1576
BsaJI	CCNNGG	3 cut(s)	960, 1233, 1281
BsaWI	WCCGGW	2 cut(s)	705, 1352
Bsc4I	CCNNNNNNNGG	9 cut(s)	141, 381, 648, 1177, 1178, 1287, 1307, 1845, 2192
Bse118I	RCCGGY	1 cut(s)	1352
Bse1I	ACTGG	2 cut(s)	163, 1207
Bse21I	CCTNAGG	1 cut(s)	2019
Bse3DI	GCAATG	2 cut(s)	1064, 2012
Bse8I	GATNNNNATC	1 cut(s)	2094
BseCI	ATCGAT	2 cut(s)	615, 2088
BseDI	CCNNGG	3 cut(s)	960, 1233, 1281
BseGI	GGATG	6 cut(s)	263, 577, 913, 1761, 1804, 2239
BseJI	GATNNNNATC	1 cut(s)	2094
BseLI	CCNNNNNNNGG	9 cut(s)	141, 381, 648, 1177, 1178, 1287, 1307, 1845, 2192
BseMI	GCAATG	2 cut(s)	1064, 2012
BseMII	CTCAG	5 cut(s)	322, 753, 782, 1083, 2142
BseNI	ACTGG	2 cut(s)	163, 1207
BseRI	GAGGAG	1 cut(s)	383
BseSI	GKGCMC	1 cut(s)	199
BseXI	GCAGC	6 cut(s)	755, 1031, 1198, 1216, 1369, 1702
BsgI	GTGCAG	1 cut(s)	762
Bsh1236I	CGCG	1 cut(s)	1235
Bsh1285I	CGRYCG	1 cut(s)	615
BshFI	GGCC	8 cut(s)	141, 197, 440, 679, 1164, 1849, 1996, 2312
BshNI	GGYRCC	1 cut(s)	125
BshTI	ACCGGT	1 cut(s)	1352
BshVI	ATCGAT	2 cut(s)	615, 2088
BsiEI	CGRYCG	1 cut(s)	615
BsiHKAI	GWGCWC	2 cut(s)	1653, 2330
BsiSI	CCGG	9 cut(s)	142, 174, 480, 566, 696, 706, 863, 1281, 1353
BslFI	GGGAC	4 cut(s)	867, 1651, 1737, 2119
BslI	CCNNNNNNNGG	9 cut(s)	141, 381, 648, 1177, 1178, 1287, 1307, 1845, 2192
BsmAI	GTCTC	4 cut(s)	784, 1576, 1825, 2138
BsmFI	GGGAC	4 cut(s)	867, 1651, 1737, 2119
BsnI	GGCC	8 cut(s)	141, 197, 440, 679, 1164, 1849, 1996, 2312
Bso31I	GGTCTC	1 cut(s)	1576
Bsp120I	GGGCCC	1 cut(s)	195
Bsp1286I	GDGCHC	3 cut(s)	199, 1653, 2330
Bsp1407I	TGTACA	1 cut(s)	619
BspACI	CCGC	6 cut(s)	375, 693, 1233, 1235, 1301, 1921
BspANI	GGCC	8 cut(s)	141, 197, 440, 679, 1164, 1849, 1996, 2312
BspCNI	CTCAG	5 cut(s)	321, 754, 781, 1084, 2143
BspDI	ATCGAT	2 cut(s)	615, 2088
BspFNI	CGCG	1 cut(s)	1235
BspLI	GGNNCC	8 cut(s)	127, 197, 855, 999, 1165, 1848, 1908, 2210
BspPI	GGATC	2 cut(s)	1482, 2084
BspQI	GCTCTTC	2 cut(s)	265, 2229
BspT107I	GGYRCC	1 cut(s)	125
BspTI	CTTAAG	1 cut(s)	2099
BspTNI	GGTCTC	1 cut(s)	1576
BsrDI	GCAATG	2 cut(s)	1064, 2012
BsrFI	RCCGGY	1 cut(s)	1352
BsrGI	TGTACA	1 cut(s)	619
BsrI	ACTGG	2 cut(s)	163, 1207
BssAI	RCCGGY	1 cut(s)	1352
BssECI	CCNNGG	3 cut(s)	960, 1233, 1281
BssT1I	CCWWGG	1 cut(s)	960
Bst4CI	ACNGT	6 cut(s)	122, 836, 1056, 2057, 2371, 2385
Bst6I	CTCTTC	7 cut(s)	222, 265, 514, 1259, 2079, 2229, 2316
BstAFI	CTTAAG	1 cut(s)	2099
BstAPI	GCANNNNNTGC	2 cut(s)	2006, 2282
BstAUI	TGTACA	1 cut(s)	619
BstC8I	GCNNGC	5 cut(s)	438, 681, 1162, 1925, 1998
BstDEI	CTNAG	9 cut(s)	65, 308, 762, 768, 1092, 1553, 1643, 2019, 2151
BstDSI	CCRYGG	1 cut(s)	1233
BstEII	GGTNACC	1 cut(s)	955
BstF5I	GGATG	6 cut(s)	263, 577, 913, 1761, 1804, 2239
BstFNI	CGCG	1 cut(s)	1235
BstH2I	RGCGCY	1 cut(s)	411
BstHHI	GCGC	2 cut(s)	71, 410
BstMAI	GTCTC	4 cut(s)	784, 1576, 1825, 2138
BstMCI	CGRYCG	1 cut(s)	615
BstMWI	GCNNNNNNNGC	8 cut(s)	1549, 1997, 2006, 2023, 2045, 2147, 2282, 2309
BstNSI	RCATGY	1 cut(s)	241
BstPI	GGTNACC	1 cut(s)	955
BstSCI	CCNGG	5 cut(s)	173, 479, 695, 861, 1280
BstSLI	GKGCMC	1 cut(s)	199
BstUI	CGCG	1 cut(s)	1235
BstV1I	GCAGC	6 cut(s)	755, 1031, 1198, 1216, 1369, 1702
BstV2I	GAAGAC	1 cut(s)	75
BstX2I	RGATCY	2 cut(s)	18, 1487
BstXI	CCANNNNNNTGG	1 cut(s)	1683
BstYI	RGATCY	2 cut(s)	18, 1487
Bsu15I	ATCGAT	2 cut(s)	615, 2088
Bsu36I	CCTNAGG	1 cut(s)	2019
BsuRI	GGCC	8 cut(s)	141, 197, 440, 679, 1164, 1849, 1996, 2312
BsuTUI	ATCGAT	2 cut(s)	615, 2088
BtgI	CCRYGG	1 cut(s)	1233
BtsCI	GGATG	6 cut(s)	263, 577, 913, 1761, 1804, 2239
BtsIMutI	CAGTG	2 cut(s)	1200, 2053
Cac8I	GCNNGC	5 cut(s)	438, 681, 1162, 1925, 1998
CfoI	GCGC	2 cut(s)	71, 410
Cfr10I	RCCGGY	1 cut(s)	1352
Cfr13I	GGNCC	7 cut(s)	195, 196, 677, 854, 1163, 1847, 1906
Cfr42I	CCGCGG	1 cut(s)	1236
ClaI	ATCGAT	2 cut(s)	615, 2088
CseI	GACGC	1 cut(s)	1452
CspAI	ACCGGT	1 cut(s)	1352
CspCI	CAANNNNNGTGG	4 cut(s)	643, 678, 1455, 1490
DdeI	CTNAG	9 cut(s)	65, 308, 762, 768, 1092, 1553, 1643, 2019, 2151
DrdI	GACNNNNNNGTC	2 cut(s)	866, 2181
DriI	GACNNNNNGTC	1 cut(s)	79
DseDI	GACNNNNNNGTC	2 cut(s)	866, 2181
EaeI	YGGCCR	2 cut(s)	139, 2310
Eam1104I	CTCTTC	7 cut(s)	222, 265, 514, 1259, 2079, 2229, 2316
Eam1105I	GACNNNNNGTC	1 cut(s)	79
EarI	CTCTTC	7 cut(s)	222, 265, 514, 1259, 2079, 2229, 2316
EciI	GGCGGA	1 cut(s)	682
Eco130I	CCWWGG	1 cut(s)	960
Eco147I	AGGCCT	1 cut(s)	440
Eco24I	GRGCYC	1 cut(s)	199
Eco31I	GGTCTC	1 cut(s)	1576
Eco47I	GGWCC	2 cut(s)	854, 1906
Eco57I	CTGAAG	3 cut(s)	238, 935, 2286
Eco81I	CCTNAGG	1 cut(s)	2019
Eco91I	GGTNACC	1 cut(s)	955
EcoO109I	RGGNCCY	4 cut(s)	195, 854, 1163, 1847
EcoO65I	GGTNACC	1 cut(s)	955
EcoRI	GAATTC	2 cut(s)	178, 455
EcoT14I	CCWWGG	1 cut(s)	960
EcoT38I	GRGCYC	1 cut(s)	199
ErhI	CCWWGG	1 cut(s)	960
FalI	AAGNNNNNCTT	4 cut(s)	1877, 1909, 2019, 2051
FaqI	GGGAC	4 cut(s)	867, 1651, 1737, 2119
FblI	GTMKAC	1 cut(s)	1316
Fnu4HI	GCNGC	8 cut(s)	744, 1020, 1212, 1230, 1233, 1301, 1383, 1716
FokI	GGATG	6 cut(s)	250, 564, 920, 1748, 1811, 2226
FriOI	GRGCYC	1 cut(s)	199
Fsp4HI	GCNGC	8 cut(s)	744, 1020, 1212, 1230, 1233, 1301, 1383, 1716
FspBI	CTAG	3 cut(s)	843, 1146, 1362
GlaI	GCGC	2 cut(s)	70, 409
GluI	GCNGC	8 cut(s)	744, 1020, 1212, 1230, 1233, 1301, 1383, 1716
GsuI	CTGGAG	1 cut(s)	620
HaeII	RGCGCY	1 cut(s)	411
HaeIII	GGCC	8 cut(s)	141, 197, 440, 679, 1164, 1849, 1996, 2312
HapII	CCGG	9 cut(s)	142, 174, 480, 566, 696, 706, 863, 1281, 1353
HgaI	GACGC	1 cut(s)	1452
HhaI	GCGC	2 cut(s)	71, 410
Hin6I	GCGC	2 cut(s)	69, 408
HinP1I	GCGC	2 cut(s)	69, 408
HincII	GTYRAC	3 cut(s)	871, 1467, 2358
HindII	GTYRAC	3 cut(s)	871, 1467, 2358
HpaII	CCGG	9 cut(s)	142, 174, 480, 566, 696, 706, 863, 1281, 1353
HphI	GGTGA	8 cut(s)	279, 493, 565, 949, 1055, 1850, 1894, 2207
Hpy188III	TCNNGA	7 cut(s)	637, 781, 1332, 1537, 2078, 2120, 2243
Hpy99I	CGWCG	1 cut(s)	190
HpyAV	CCTTC	7 cut(s)	222, 231, 336, 1429, 1592, 1879, 2196
HpyCH4III	ACNGT	6 cut(s)	122, 836, 1056, 2057, 2371, 2385
HpyCH4IV	ACGT	2 cut(s)	298, 1313
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1549, 1997, 2006, 2023, 2045, 2147, 2282, 2309
HpyF3I	CTNAG	9 cut(s)	65, 308, 762, 768, 1092, 1553, 1643, 2019, 2151
HpySE526I	ACGT	2 cut(s)	298, 1313
HspAI	GCGC	2 cut(s)	69, 408
KspI	CCGCGG	1 cut(s)	1236
LguI	GCTCTTC	2 cut(s)	265, 2229
LmnI	GCTCC	4 cut(s)	1460, 1648, 1712, 2023
Lsp1109I	GCAGC	6 cut(s)	755, 1031, 1198, 1216, 1369, 1702
LweI	GCATC	5 cut(s)	898, 1093, 1530, 1996, 2272
MaeI	CTAG	3 cut(s)	843, 1146, 1362
MaeII	ACGT	2 cut(s)	298, 1313
MaeIII	GTNAC	6 cut(s)	370, 513, 754, 933, 955, 1198
MfeI	CAATTG	2 cut(s)	1931, 2228
MflI	RGATCY	2 cut(s)	18, 1487
MhlI	GDGCHC	3 cut(s)	199, 1653, 2330
MlsI	TGGCCA	1 cut(s)	2312
MluNI	TGGCCA	1 cut(s)	2312
MlyI	GAGTC	9 cut(s)	68, 306, 350, 485, 625, 683, 1206, 1286, 1633
MmeI	TCCRAC	4 cut(s)	33, 378, 574, 1677
Mox20I	TGGCCA	1 cut(s)	2312
MscI	TGGCCA	1 cut(s)	2312
MseI	TTAA	7 cut(s)	102, 702, 1113, 1131, 1515, 2100, 2130
MslI	CAYNNNNRTG	4 cut(s)	942, 1681, 1743, 1770
Msp20I	TGGCCA	1 cut(s)	2312
MspA1I	CMGCKG	3 cut(s)	1235, 1303, 2150
MspCI	CTTAAG	1 cut(s)	2099
MspI	CCGG	9 cut(s)	142, 174, 480, 566, 696, 706, 863, 1281, 1353
MspR9I	CCNGG	5 cut(s)	175, 481, 697, 863, 1282
MunI	CAATTG	2 cut(s)	1931, 2228
MvnI	CGCG	1 cut(s)	1235
MwoI	GCNNNNNNNGC	8 cut(s)	1549, 1997, 2006, 2023, 2045, 2147, 2282, 2309
NciI	CCSGG	5 cut(s)	175, 481, 697, 863, 1282
NlaIV	GGNNCC	8 cut(s)	127, 197, 855, 999, 1165, 1848, 1908, 2210
NmuCI	GTSAC	5 cut(s)	370, 513, 754, 955, 1198
NspI	RCATGY	1 cut(s)	241
PceI	AGGCCT	1 cut(s)	440
PciI	ACATGT	1 cut(s)	237
PciSI	GCTCTTC	2 cut(s)	265, 2229
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 2085
PfeI	GAWTC	8 cut(s)	131, 158, 249, 484, 597, 722, 809, 2392
PfoI	TCCNGGA	2 cut(s)	173, 479
PinAI	ACCGGT	1 cut(s)	1352
PkrI	GCNGC	8 cut(s)	745, 1021, 1213, 1231, 1234, 1302, 1384, 1717
Ple19I	CGATCG	1 cut(s)	615
PleI	GAGTC	9 cut(s)	68, 306, 350, 484, 625, 683, 1205, 1285, 1633
PpsI	GAGTC	9 cut(s)	68, 306, 350, 484, 625, 683, 1205, 1285, 1633
PpuMI	RGGWCCY	1 cut(s)	854
PscI	ACATGT	1 cut(s)	237
PsiI	TTATAA	1 cut(s)	1008
Psp5II	RGGWCCY	1 cut(s)	854
PspEI	GGTNACC	1 cut(s)	955
PspN4I	GGNNCC	8 cut(s)	127, 197, 855, 999, 1165, 1848, 1908, 2210
PspOMI	GGGCCC	1 cut(s)	195
PspPI	GGNCC	7 cut(s)	195, 196, 677, 854, 1163, 1847, 1906
PspPPI	RGGWCCY	1 cut(s)	854
PsuI	RGATCY	2 cut(s)	18, 1487
PvuI	CGATCG	1 cut(s)	615
PvuII	CAGCTG	1 cut(s)	2150
RseI	CAYNNNNRTG	4 cut(s)	942, 1681, 1743, 1770
SacII	CCGCGG	1 cut(s)	1236
SapI	GCTCTTC	2 cut(s)	265, 2229
SaqAI	TTAA	7 cut(s)	102, 702, 1113, 1131, 1515, 2100, 2130
SatI	GCNGC	8 cut(s)	744, 1020, 1212, 1230, 1233, 1301, 1383, 1716
Sau96I	GGNCC	7 cut(s)	195, 196, 677, 854, 1163, 1847, 1906
ScaI	AGTACT	3 cut(s)	838, 1360, 2368
SchI	GAGTC	9 cut(s)	68, 306, 350, 485, 625, 683, 1206, 1286, 1633
ScrFI	CCNGG	5 cut(s)	175, 481, 697, 863, 1282
SduI	GDGCHC	3 cut(s)	199, 1653, 2330
SfaNI	GCATC	5 cut(s)	898, 1093, 1530, 1996, 2272
Sfr303I	CCGCGG	1 cut(s)	1236
SgrBI	CCGCGG	1 cut(s)	1236
SinI	GGWCC	2 cut(s)	854, 1906
SmiMI	CAYNNNNRTG	4 cut(s)	942, 1681, 1743, 1770
SmlI	CTYRAG	2 cut(s)	2099, 2213
SmoI	CTYRAG	2 cut(s)	2099, 2213
SpeI	ACTAGT	1 cut(s)	1145
SseBI	AGGCCT	1 cut(s)	440
SsiI	CCGC	6 cut(s)	375, 693, 1233, 1235, 1301, 1921
SspMI	CTAG	3 cut(s)	843, 1146, 1362
StuI	AGGCCT	1 cut(s)	440
StyD4I	CCNGG	5 cut(s)	173, 479, 695, 861, 1280
StyI	CCWWGG	1 cut(s)	960
TaaI	ACNGT	6 cut(s)	122, 836, 1056, 2057, 2371, 2385
TaiI	ACGT	2 cut(s)	301, 1316
TatI	WGTACW	5 cut(s)	619, 836, 1358, 1874, 2366
TauI	GCSGC	2 cut(s)	1235, 1303
TfiI	GAWTC	8 cut(s)	131, 158, 249, 484, 597, 722, 809, 2392
Tru1I	TTAA	7 cut(s)	102, 702, 1113, 1131, 1515, 2100, 2130
Tru9I	TTAA	7 cut(s)	102, 702, 1113, 1131, 1515, 2100, 2130
TscAI	CASTG	2 cut(s)	1207, 2060
TseFI	GTSAC	5 cut(s)	370, 513, 754, 955, 1198
TseI	GCWGC	6 cut(s)	743, 1019, 1211, 1229, 1382, 1715
Tsp45I	GTSAC	5 cut(s)	370, 513, 754, 955, 1198
TspDTI	ATGAA	4 cut(s)	252, 618, 1100, 1727
TspGWI	ACGGA	1 cut(s)	1484
TspRI	CASTG	2 cut(s)	1207, 2060
Vha464I	CTTAAG	1 cut(s)	2099
VpaK11BI	GGWCC	2 cut(s)	854, 1906
XapI	RAATTY	5 cut(s)	178, 275, 455, 1701, 2135
XceI	RCATGY	1 cut(s)	241
XmiI	GTMKAC	1 cut(s)	1316
XspI	CTAG	3 cut(s)	843, 1146, 1362
ZrmI	AGTACT	3 cut(s)	838, 1360, 2368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.