MD04G1231700.v1.1

Caleosin related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011702
Physical Location & Seq
Forward (+)
31112645 .. 31114475
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1231700.v1.1.491

Sequence Viewer

Length: 735 bp
ATGGCAGTAGATCAGGGCCAGCAGCAGGCATCCAGTGAGTCCATGGCAACGGTGGCTGAGAAGGCTCCGATCACCGCGGAGAGGAAGGTCCGCAATGATTTGGAGACCAAGCTCCCCAAACCATACATGCCTAGGGCTATGATTGCGCCTGATATGGAGAACATCAATGGCACATCGGGCCATAAGCATTCCAACATGTCTGTGCTTCAACAGCATGCAGCTTTCTTCGACCAAGACAACGATGGCATAATCTACCCTTCTGAAACATTCAGAGGATTTCGCGCCCTCGGGTTTAATCCGGTTGCTTCCTTCCTTTTCATGGTTCTTGTCCATGCAGCTATGAGTTATGCTACTCTTCCTACATGGATACCGTCGCCTTTCTTCGCAATTCACATAAAGAACATACACATGGCAAAGCATGGAAGTGACTCAGGGACGTATGACACAGAGGGACGGTACATTCCTGCAAATCTGGAGAACATGTTCAGCAAGTATGCCCGCACCGTGCCTGATAAGCTTTCATTCAAGGAGCTTTGGCACATGACTCAGGCTAACCGTGACGCCTTTGATTTCTTTGGCTGGATTGCAAGTAAACTGGAATGGGGAGTTCTTTATGTTCTCGCAAAAGATGAAAACGGCTACTTGGCAAAGGAAGCTGTGAGACGCTGTTTCGATGGAAGCTTGTTCGAGTACTGTTCAAAGTTGCAGAAGGGCGCTGTTGGTAAGATGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
Caleosin

Protein Analysis

245

Amino Acids

27.42

Weight (kDa)

7.1

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Caleosin PF05042 65 - 232 7.7e-78 Caleosin related protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 77, 282
AciI CCGC 4 cut(s) 75, 77, 91, 499
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 2 cut(s) 458, 692
AfiI CCNNNNNNNGG 3 cut(s) 25, 81, 319
AflIII ACRYGT 2 cut(s) 195, 480
AgsI TTSAA 3 cut(s) 209, 526, 699
AloI GAACNNNNNNTCC 2 cut(s) 467, 499
AluBI AGCT 7 cut(s) 112, 221, 338, 517, 532, 656, 681
AluI AGCT 7 cut(s) 112, 221, 338, 517, 532, 656, 681
Alw26I GTCTC 2 cut(s) 98, 655
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 2 cut(s) 16, 178
ApeKI GCWGC 3 cut(s) 22, 218, 335
Asp700I GAANNNNTTC 1 cut(s) 482
AspA2I CCTAGG 1 cut(s) 131
AspLEI GCGC 3 cut(s) 148, 284, 716
AspS9I GGNCC 3 cut(s) 16, 88, 178
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 88
AvrII CCTAGG 1 cut(s) 131
BbvI GCAGC 3 cut(s) 34, 230, 347
BccI CCATC 3 cut(s) 236, 668, 721
BceAI ACGGC 1 cut(s) 652
BciVI GTATCC 1 cut(s) 360
BcoDI GTCTC 2 cut(s) 98, 655
BfaI CTAG 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 717
BfuI GTATCC 1 cut(s) 360
BisI GCNGC 3 cut(s) 23, 219, 336
BlnI CCTAGG 1 cut(s) 131
BlsI GCNGC 3 cut(s) 24, 220, 337
BmcAI AGTACT 1 cut(s) 692
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 3 cut(s) 16, 88, 178
BmiI GGNNCC 1 cut(s) 66
BmsI GCATC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 494
BsaHI GRCGYC 1 cut(s) 561
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 4 cut(s) 42, 75, 131, 286
BsaWI WCCGGW 1 cut(s) 298
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 81, 319
Bse1I ACTGG 2 cut(s) 33, 600
Bse3DI GCAATG 1 cut(s) 100
BseDI CCNNGG 4 cut(s) 42, 75, 131, 286
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 3 cut(s) 25, 81, 319
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 3 cut(s) 48, 444, 560
BseNI ACTGG 2 cut(s) 33, 600
BseXI GCAGC 3 cut(s) 34, 230, 347
Bsh1236I CGCG 2 cut(s) 77, 282
BshFI GGCC 2 cut(s) 18, 180
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 2 cut(s) 448, 465
BslI CCNNNNNNNGG 3 cut(s) 25, 81, 319
BsmAI GTCTC 2 cut(s) 98, 655
BsmBI CGTCTC 1 cut(s) 655
BsmFI GGGAC 2 cut(s) 448, 465
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 2 cut(s) 18, 180
Bso31I GGTCTC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 287
Bsp143I GATC 2 cut(s) 10, 69
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 4 cut(s) 75, 77, 91, 499
BspANI GGCC 2 cut(s) 18, 180
BspCNI CTCAG 3 cut(s) 49, 443, 559
BspFNI CGCG 2 cut(s) 77, 282
BspLI GGNNCC 1 cut(s) 66
BspTNI GGTCTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 100
BsrI ACTGG 2 cut(s) 33, 600
BssECI CCNNGG 4 cut(s) 42, 75, 131, 286
BssMI GATC 2 cut(s) 10, 69
BssNI GRCGYC 1 cut(s) 561
BssT1I CCWWGG 2 cut(s) 42, 131
Bst4CI ACNGT 6 cut(s) 52, 372, 456, 505, 557, 695
Bst6I CTCTTC 1 cut(s) 360
BstACI GRCGYC 1 cut(s) 561
BstC8I GCNNGC 4 cut(s) 20, 27, 216, 499
BstDEI CTNAG 3 cut(s) 57, 430, 546
BstDSI CCRYGG 2 cut(s) 42, 75
BstF5I GGATG 1 cut(s) 29
BstFNI CGCG 2 cut(s) 77, 282
BstH2I RGCGCY 1 cut(s) 717
BstHHI GCGC 3 cut(s) 148, 284, 716
BstKTI GATC 2 cut(s) 13, 72
BstMAI GTCTC 2 cut(s) 98, 655
BstMBI GATC 2 cut(s) 10, 69
BstMWI GCNNNNNNNGC 7 cut(s) 53, 62, 143, 177, 211, 514, 653
BstNSI RCATGY 4 cut(s) 130, 199, 218, 484
BstUI CGCG 2 cut(s) 77, 282
BstV1I GCAGC 3 cut(s) 34, 230, 347
BsuI GTATCC 1 cut(s) 360
BsuRI GGCC 2 cut(s) 18, 180
BtgI CCRYGG 2 cut(s) 42, 75
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 4 cut(s) 20, 27, 216, 499
CfoI GCGC 3 cut(s) 148, 284, 716
Cfr13I GGNCC 3 cut(s) 16, 88, 178
Cfr42I CCGCGG 1 cut(s) 78
CseI GACGC 2 cut(s) 569, 672
Csp6I GTAC 2 cut(s) 457, 691
CviQI GTAC 2 cut(s) 457, 691
DdeI CTNAG 3 cut(s) 57, 430, 546
DpnI GATC 2 cut(s) 12, 71
DpnII GATC 2 cut(s) 10, 69
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
Eco130I CCWWGG 2 cut(s) 42, 131
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 88
Eco88I CYCGRG 1 cut(s) 287
EcoT14I CCWWGG 2 cut(s) 42, 131
ErhI CCWWGG 2 cut(s) 42, 131
Esp3I CGTCTC 1 cut(s) 655
FaqI GGGAC 2 cut(s) 448, 465
FauI CCCGC 1 cut(s) 506
Fnu4HI GCNGC 3 cut(s) 23, 219, 336
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 3 cut(s) 23, 219, 336
FspBI CTAG 1 cut(s) 132
GlaI GCGC 3 cut(s) 147, 283, 715
GluI GCNGC 3 cut(s) 23, 219, 336
GsuI CTGGAG 1 cut(s) 494
HaeII RGCGCY 1 cut(s) 717
HaeIII GGCC 2 cut(s) 18, 180
HapII CCGG 1 cut(s) 299
HgaI GACGC 2 cut(s) 569, 672
HhaI GCGC 3 cut(s) 148, 284, 716
Hin1I GRCGYC 1 cut(s) 561
Hin6I GCGC 3 cut(s) 146, 282, 714
HinP1I GCGC 3 cut(s) 146, 282, 714
HindIII AAGCTT 2 cut(s) 515, 679
HinfI GANTC 3 cut(s) 38, 428, 544
HpaII CCGG 1 cut(s) 299
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 1 cut(s) 593
Hpy188I TCNGA 3 cut(s) 69, 262, 272
Hpy188III TCNNGA 1 cut(s) 473
Hpy8I GTNNAC 1 cut(s) 593
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 5 cut(s) 55, 79, 267, 319, 703
HpyCH4III ACNGT 6 cut(s) 52, 372, 456, 505, 557, 695
HpyCH4IV ACGT 1 cut(s) 437
HpyCH4V TGCA 5 cut(s) 218, 335, 467, 587, 706
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 62, 143, 177, 211, 514, 653
HpyF3I CTNAG 3 cut(s) 57, 430, 546
HpySE526I ACGT 1 cut(s) 437
Hsp92I GRCGYC 1 cut(s) 561
HspAI GCGC 3 cut(s) 146, 282, 714
KspI CCGCGG 1 cut(s) 78
Kzo9I GATC 2 cut(s) 10, 69
LmnI GCTCC 3 cut(s) 70, 117, 529
Lsp1109I GCAGC 3 cut(s) 34, 230, 347
LweI GCATC 1 cut(s) 38
MaeI CTAG 1 cut(s) 132
MaeII ACGT 1 cut(s) 437
MaeIII GTNAC 2 cut(s) 425, 557
MalI GATC 2 cut(s) 12, 71
MboI GATC 2 cut(s) 10, 69
MboII GAAGA 3 cut(s) 217, 347, 373
MluCI AATT 1 cut(s) 387
MlyI GAGTC 3 cut(s) 47, 422, 538
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 4 cut(s) 75, 266, 296, 442
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 3 cut(s) 200, 407, 423
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 1 cut(s) 299
Mva1269I GAATGC 1 cut(s) 187
MvnI CGCG 2 cut(s) 77, 282
MwoI GCNNNNNNNGC 7 cut(s) 53, 62, 143, 177, 211, 514, 653
NcoI CCATGG 1 cut(s) 42
NdeII GATC 2 cut(s) 10, 69
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 2 cut(s) 425, 557
NspI RCATGY 4 cut(s) 130, 199, 218, 484
PaeI GCATGC 1 cut(s) 218
PciI ACATGT 2 cut(s) 195, 480
PctI GAATGC 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 482
PkrI GCNGC 3 cut(s) 24, 220, 337
PleI GAGTC 3 cut(s) 46, 422, 538
PpsI GAGTC 3 cut(s) 46, 422, 538
PscI ACATGT 2 cut(s) 195, 480
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 3 cut(s) 16, 88, 178
RsaI GTAC 2 cut(s) 458, 692
RsaNI GTAC 2 cut(s) 457, 691
RseI CAYNNNNRTG 3 cut(s) 200, 407, 423
SacII CCGCGG 1 cut(s) 78
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 3 cut(s) 23, 219, 336
Sau3AI GATC 2 cut(s) 10, 69
Sau96I GGNCC 3 cut(s) 16, 88, 178
ScaI AGTACT 1 cut(s) 692
SchI GAGTC 3 cut(s) 47, 422, 538
SetI ASST 9 cut(s) 90, 114, 223, 340, 440, 519, 534, 658, 683
SfaNI GCATC 1 cut(s) 38
Sfr303I CCGCGG 1 cut(s) 78
SgrBI CCGCGG 1 cut(s) 78
SinI GGWCC 1 cut(s) 88
SmiMI CAYNNNNRTG 3 cut(s) 200, 407, 423
SphI GCATGC 1 cut(s) 218
Sse9I AATT 1 cut(s) 387
SsiI CCGC 4 cut(s) 75, 77, 91, 499
SspMI CTAG 1 cut(s) 132
StyI CCWWGG 2 cut(s) 42, 131
TaaI ACNGT 6 cut(s) 52, 372, 456, 505, 557, 695
TaiI ACGT 1 cut(s) 440
TaqI TCGA 3 cut(s) 228, 672, 687
TasI AATT 1 cut(s) 387
TatI WGTACW 1 cut(s) 690
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 2 cut(s) 425, 557
TseI GCWGC 3 cut(s) 22, 218, 335
Tsp45I GTSAC 2 cut(s) 425, 557
TspDTI ATGAA 3 cut(s) 307, 510, 645
TspRI CASTG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 88
XceI RCATGY 4 cut(s) 130, 199, 218, 484
XcmI CCANNNNNNNNNTGG 3 cut(s) 40, 49, 239
XmaJI CCTAGG 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 1 cut(s) 132
ZrmI AGTACT 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.